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Journal articles on the topic 'Biochemical identification of salmonella paratyphi'

Author: Grafiati

Published: 3 June 2025

Last updated: 31 July 2025

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1

Kim, Gyu Ri, Si Hyun Kim, Eun-Young Kim, et al. "Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification of Salmonella Species." Microorganisms 10, no. 10 (2022): 1974. http://dx.doi.org/10.3390/microorganisms10101974.

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Salmonella is a major pathogen causing foodborne infections in humans. Salmonella isolates are identified using biochemical and serological tests, including automated systems such as the VITEK2 system. However, there are few reports on Salmonella identification using VITEK MS. Therefore, we aimed to evaluate the usefulness of MALDI-TOF VITEK MS for Salmonella identification. A total of 1389 Salmonella isolates were identified using VITEK MS ver3.0 or ver3.2. All Salmonella isolates were confirmed by serotyping using the Kauffmann-White scheme, and the results were compared with the VITEK MS re

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Cordovana, Miriam, Norman Mauder, Markus Kostrzewa, et al. "Classification of Salmonella enterica of the (Para-)Typhoid Fever Group by Fourier-Transform Infrared (FTIR) Spectroscopy." Microorganisms 9, no. 4 (2021): 853. http://dx.doi.org/10.3390/microorganisms9040853.

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Typhoidal and para-typhoidal Salmonella are major causes of bacteraemia in resource-limited countries. Diagnostic alternatives to laborious and resource-demanding serotyping are essential. Fourier transform infrared spectroscopy (FTIRS) is a rapidly developing and simple bacterial typing technology. In this study, we assessed the discriminatory power of the FTIRS-based IR Biotyper (Bruker Daltonik GmbH, Bremen, Germany), for the rapid and reliable identification of biochemically confirmed typhoid and paratyphoid fever-associated Salmonella isolates. In total, 359 isolates, comprising 30 S. Typ

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Suman, Maiz-ul Ahad, Md Abdullah Siddique, S. M. Shamsuzzaman, et al. "Detection Of Mutated gyrA Gene From Nalidixic Acid Resistant Salmonella Typhi And Paratyphi A Isolated From Enteric Fever Patients In A Tertiary Care Hospital Of Bangladesh." Bangladesh Journal of Medical Microbiology 10, no. 1 (2017): 3–7. http://dx.doi.org/10.3329/bjmm.v10i1.31431.

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A cross sectional study for the detection of empirically used antibiotic resistant Salmonella was carried out in the department of microbiology of Rajshahi Medical College (RMC) and PCR and DNA sequencing were done in the department of microbiology of Dhaka Medical College during the period from August 2014 to July 2015.Total 323 blood samples were collected from suspected enteric fever patients from medicine and paediatric units of RMC hospital and cultured on brain heart infusion broth for isolation of Salmonella. Identification of Salmonella was done by biochemical tests and final identific

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Bakhrouf, Amina, Moncef Jeddi, and Michel J. Gauthier. "Modification des caractères culturaux et biochimiques du Salmonella paratyphi B après incubation dans l'eau de mer." Canadian Journal of Microbiology 38, no. 8 (1992): 871–74. http://dx.doi.org/10.1139/m92-142.

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After incubation in seawater Salmonella paratyphi B cells rapidly became unable to grow on bacteriological media. Previous adaptation to high osmolarity conditions greatly slowed down this process. Strains isolated from seawater microcosms after varying incubation periods were qualitatively different and showed changes in some of their growth (colony shape and size) and biochemical properties (acidification of some sugars, gelatinase activity, acetoin production, nitrate reduction). Because of these modifications, the bacteria showed atypical profiles and could not be identified as members of

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Chmel, Martin, Oldřich Bartoš, Ondřej Beran, et al. "Salmonella Paratyphi Infection: Use of Nanopore Sequencing as a Vivid Alternative for the Identification of Invading Bacteria." Prague Medical Report 122, no. 2 (2021): 96–105. http://dx.doi.org/10.14712/23362936.2021.10.

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In our study we present an overview of the use of Oxford Nanopore Technologies (ONT) sequencing technology on the background of Enteric fever. Unlike traditional methods (e.g., qPCR, serological tests), the nanopore sequencing technology enables virtually real-time data generation and highly accurate pathogen identification and characterization. Blood cultures were obtained from a 48-year-old female patient suffering from a high fever, headache and diarrhea. Nevertheless, both the initial serological tests and stool culture appeared to be negative. Therefore, the bacterial isolate from blood c

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Ijaz, Samia, Rifat Bibi, Nazia, et al. "Biochemical Characterization and Antibiotic Profile against Salmonella Species Isolated from Milk and Milk Products." Open Access Public Health and Health Administration Review 3, no. 2 (2025): 156–64. https://doi.org/10.59644/oaphhar.3(2).176.

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Salmonella species are among the most common foodborne pathogens associated with milk and dairy products. This study investigated the microbiological characteristics and antibiotic resistance patterns of Salmonella spp. in milk, ice cream, and cheese samples collected from August to December 2024 in Layyah District, Punjab, Pakistan. A total of 150 randomly selected samples (50 each of milk, ice cream, and cheese) were analyzed. Isolation and identification were performed using standard biochemical tests and further confirmed using the API 10S identification kit. Salmonella contamination was d

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Agrawal, P., R. Tuladhar, and N. Dahal. "Nalidixic Acid Susceptibility Test for Screening Salmonella Isolates of Reduced Susceptibility/Higher Minimum Inhibitory Concentration to Ciprofloxacin." Nepal Journal of Science and Technology 15, no. 2 (2015): 97–104. http://dx.doi.org/10.3126/njst.v15i2.12122.

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Enteric fever is the major diagnosis among febrile patients in Nepal with yearly increase in nalidixic acid resistance and reduced ciprofloxacin susceptibility among Salmonella isolates. This study was carried out to evaluate the validity of nalidixic acid resistance as an indicator of reduced susceptibility of Salmonella isolates to ciprofloxacin. In this study, 999 blood specimens collected from suspected enteric fever patients visiting B&B Hospital were processed by standard microbiological techniques. Isolates were identified by biochemical tests and serotyping. Antibiotic susceptibili

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Niroula, Dhirendra, Jyotsna Shrestha, Supriya Sharma, and Anjana Singh. "Antibiotic Susceptibility Pattern of Salmonella Enterica serovars Typhi and Paratyphi A Isolated From Patients Suspected of Enteric Fever." Tribhuvan University Journal of Microbiology 7 (December 26, 2020): 31–36. http://dx.doi.org/10.3126/tujm.v7i0.33791.

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Objectives: The study aimed to assess the antibiotic susceptibility profile of Salmonella spp isolated from patients suspected of enteric fever. Methods: This cross-sectional prospective study was carried out from April to June, 2014among 484 patients clinically suspected of enteric fever visiting Bir Hospital, Kathmandu, Nepal. Blood sample collected from each patient was processed for culture in bile broth. Identification of Salmonella spp was done by conventional microbiological techniques including colony characteristics, Gram's staining and biochemical tests. Antibiotic susceptibili

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Olorunsola, R. A., D. Eruvbetine, O. M. Idowu, O. Oyekunle, and I. M. Ogunade. "Salmonella organism transmission in hatching broiler egggs." Nigerian Journal of Animal Production 41, no. 1 (2021): 230–34. http://dx.doi.org/10.51791/njap.v41i1.2729.

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The vertical transmission of Salmonella organism in hatching broiler eggs were investigated in selected states in South-Western Nigeria. Hatching eggs were obtained from fifteen major hatcheries. five (hatchery A, B, C, D and E from each State [Lagos (LA), Oyo (OY) and Ogun (OG)]. A total of 300 hatchable eggs of 20 eggs from each hatchery were collected. Individual egg was broken and separated into Shell, albumin and yolk. The samples were incubated into buffer peptone water and incubated at 37°C for 24 hours. Typical colonies of salmonella grown on Xylose lysine deoxycholate agar were seen a

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Fahima Akter and Md Shamimuzzaman. "DETECTION OF INVA GENE BY PCR AND IDENTIFICATION WITH SUSCEPTIBILITY PATTERN EVALUATION BY VITEK 2 OF SALMONELLA ISOLATES." Journal of Applied Biological Sciences 18, no. 3 (2024): 235–52. https://doi.org/10.71336/jabs.1313.

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This study aims to identify and antimicrobial susceptibility of hospital wastewater in Dhaka city. Total 134 Salmonella isolates were identified using cultural characteristics, automated biochemical test, and PCR for invA gene detection. Biochemical identification and Antimicrobial Susceptibility Test (AST) were performed through VITEK 2. Essential Agreement (EA) and Categorical Agreement (CA) were worked out according to the CLSI breakpoint. We also measured Very Major Error (VME), Major Error (ME), and Minor Error (mE). Seven Salmonella serotypes were identified through VITEK 2 ID-GN card an

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Akter, Fahima, and Md. Shamimuzzaman. "DETECTION OF INVA GENE BY PCR AND IDENTIFICATION WITH SUSCEPTIBILITY PATTERN EVALUATION BY VITEK 2 OF SALMONELLA ISOLATES." Journal of Applied Biological Sciences 18, no. 3 (2024): 235–52. https://doi.org/10.5281/zenodo.13857861.

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This study aims to identify and antimicrobial susceptibility of hospital wastewater in Dhaka city. Total 134 <em>Salmonella</em> isolates were identified using cultural characteristics, automated biochemical test, and PCR for <em>invA </em>gene detection. Biochemical identification and Antimicrobial Susceptibility Test (AST) were performed through VITEK 2. Essential Agreement (EA) and Categorical Agreement (CA) were worked out according to the CLSI breakpoint. We also measured Very Major Error (VME), Major Error (ME), and Minor Error (mE). Seven <em>Salmonella </em>serotypes were identified th

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Hasan, Thamer O., Inam J Lafta, Emad A Ahmed, and Samah A Jassam. "Application of RAPD-PCR and Phylogenetic Analysis for Accurate ‎Characterization of ‎Salmonella‎‎‎ spp. Isolated from Chicken and Their Feed ‎and Drinking Water in Comparison ‎with API 20E, Vitek 2, and Serotyping." Iraqi Journal of Veterinary Medicine 47, no. 1 (2023): 11–20. http://dx.doi.org/10.30539/ijvm.v47i1.1493.

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    The aim of this study was ‎the‎ discrimination of Salmonella‎‎ isolated from chicken and their feed ‎and drinking water for the epidemiological control of salmonellosis. Totally, 289 samples, ‎including 217 chicken cloaca swabs, 46 water, and 26 feed samples were collected from five ‎different farms in Karbala governorate, Iraq. Conventional bacteriology tests, API 20E, Vitek 2, ‎and serology were used for bacterial identification. Random amplified polymorphic ‎DNA (RAPD)-polymerase chain reaction (PCR) was applied to analyze the genetic relationships ‎among Salmone

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Dauphin Dighitoghi Moro, Igbagbo Ehinmore, Oluchi Mariam Ukhureigbe, Jyostna Chandwani, and Kabiru Olusegun Akinyemi. "Serotyping and antibiotic susceptibility pattern of Salmonella serovars from children diagnosed of typhoid fever in Lagos, Nigeria." Open Access Research Journal of Science and Technology 4, no. 2 (2022): 058–65. http://dx.doi.org/10.53022/oarjst.2022.4.2.0034.

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Typhoid fever is a major health problem in developing countries especially in Nigeria where this infectious disease is endemic, with more concern in children. This prospective study was conducted in 3 Primary Health Centres on children diagnosed clinically of typhoid fever between January, 2017 and December, 2019. The aim of this study was to determine the incidence of Salmonella serovars and antibiotic susceptibility pattern from faecal specimens of children diagnosed clinically based on symptoms of typhoid fever, while demographic information was recovered from their case notes and their mot

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Baibhav, R. Barbaruah R. Roy U. Chetri and SR Joshi. "Currency notes in circulation can be potential vectors for transmissible diseases." NEHU International Journal l Vol. XIX, No. 2, (July –December), 2021 (2022): 82–96. https://doi.org/10.5281/zenodo.7098305.

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In the modern era, currency is the primary medium for exchange of goods and services. The material of Indian currency notes makes it a hub for microorganism to thrive. Taxi drivers exchange currency with customers for different regions and backgrounds and meat sellers are known to use bare hands for butchering of the meat and exchanging currency with customers without washing or sanitizing. This paper highlights the presence of multitude of bacteria which have the potential to cause diseases as well as being antibiotic resistant. 20 different strains were isolated, 10 each from currency notes

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Tinrat, Sirikhwan, and Saranya Sedtananun. "NOVEL RUMMELIIBACILLUS SP. ISOLATED FROM FERMENTED VEGETABLE PRODUCTS AS THE POTENTIAL PROBIOTICS." Journal of microbiology, biotechnology and food sciences 11, no. 5 (2022): e4194. http://dx.doi.org/10.55251/jmbfs.4194.

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The novel lactic acid bacteria has potential probiotics which were selectively screened from different fermented vegetable products by antagonistic activity against Escherichia coli ATCC 25922 as a food-borne pathogenic bacteria. From 40 isolates on MRS agar, some isolates had anti-E. coli activity. Surprisingly, isolated STR 0103f and STR 0404f strains demonstrated bile salt-tolerance at 0.3% bile salt for 24 hours with survival rate of 67.77±0.38 and 68.72±0.45%, respectively. Both strains also survived under acidic conditions (pH 3-5) with 60.53±0.08-76.87±0.09% of survival rate. STR 0103f

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Alex Morel Longue Essoua, Fabrice Fabien Dongho Dongmo, Martin Luther Mogtomo Koanga, et al. "Study of coinfection of Salmonella typhi, Salmonella paratyphi with Candida spp., in four hospitals of Douala- Cameroon." International Journal of Science and Research Archive 2, no. 2 (2021): 110–19. http://dx.doi.org/10.30574/ijsra.2021.2.2.0041.

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Candida spp., are saprophytic yeasts capable of creating a favorable environment for bacterial infection, amplifying or inhibiting their activity. On the other hand, bacteria produce molecules that reduce the proliferation of Candida spp or promote their adherence to surfaces. The resurgence of typhoid and paratyphoid fevers in endemic individuals and the relative effectiveness of contemporary conventional therapies arouse curiosity to explore other factors that may influence, the re-emergence of infection in patients. We carried out a cross-sectional study of 2500 patients in four hospitals i

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Shetty, Anup Kumar, Ichlampady Nagaraj Shetty, Zevita Venisha Furtado, Beena Antony, and Rekha Boloor. "Antibiogram of Salmonella Isolates from Blood with an Emphasis on Nalidixic Acid and Chloramphenicol Susceptibility in a Tertiary Care Hospital in Coastal Karnataka: A Prospective Study." Journal of Laboratory Physicians 4, no. 02 (2012): 074–77. http://dx.doi.org/10.4103/0974-2727.105585.

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ABSTRACT Background: Enteric fever is caused by the serotypes Salmonella Typhi, Salmonella Paratyphi A, Salmonella Paratyphi B and Salmonella Paratyphi C. After emergence of multidrug resistant Salmonellae Ciprofloxacin, a fluorquinolone antibiotic was the first-line therapy. Treatment failure was observed with Ciprofloxacin soon and such strains showed in-vitro resistance to Nalidixic acid. Recent reports suggest re-emergence of Chloramphenicol sensitive strains and increasing Nalidixic acid resistance. This study is aimed at detecting the current trend in the antibiogram of Salmonella isolat

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Rahman, Farha, Sanya Tahmina Jhora, Shikha Paul, Dipika Sarkar, and Israt Sadia. "Antimicrobial Susceptibility Pattern of Typhoidal Salmonella Species in Tertiary Hospitals of Dhaka City." International Journal of Research and Innovation in Social Science VIII, no. II (2024): 2552–63. http://dx.doi.org/10.47772/ijriss.2024.802183.

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Enteric fever caused by Salmonella enterica remains an unresolved public health problem and due to the prevalence of multidrug resistant (MDR) strains for the last two decades, the first line antimicrobials were discontinued and currently the second line antimicrobials are used. A total of 325 blood samples from clinically suspected enteric fever patients were collected during the study period of July, 2014 to June, 2015. After identification of organisms, antimicrobial susceptibility tests were done by disc diffusion method and MIC by broth microdilution method. Rate of isolation of organisms

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Admassu, Dawit, Gudina Egata, and Zelalem Teklemariam. "Prevalence and antimicrobial susceptibility pattern of Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi among febrile patients at Karamara Hospital, Jigjiga, eastern Ethiopia." SAGE Open Medicine 7 (January 2019): 205031211983785. http://dx.doi.org/10.1177/2050312119837854.

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Objective: The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi among febrile patients at Karamara Hospital, Jigjiga, eastern Ethiopia. Methods: A cross-sectional study was conducted among 203 febrile patients presumptive of enteric fever ( Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi) at Karamara Hospital from 15 February to 20 March 2016. Venous blood was collected, cultured, and biochemical tests were performed. Antimicrobial susceptibility

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Biswas, Malabika, Silpak Biswas, Bishal Gupta, Maria Teresa Mascellino, Anindita Rakshit, and Banya Chakraborty. "Changing Paradigms in Antibiotic Resistance in Salmonella Species with Focus on Fluoroquinolone Resistance: A 5-Year Retrospective Study of Enteric Fever in a Tertiary Care Hospital in Kolkata, India." Antibiotics 11, no. 10 (2022): 1308. http://dx.doi.org/10.3390/antibiotics11101308.

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Enteric fever, a potentially fatal multisystem disease that is caused by Salmonella enterica serovar Typhi and Paratyphi, poses a significant risk in low- and middle-income countries. A retrospective study to understand the prevalence and evolving patterns of antibiotic resistance in Salmonella Typhi and Paratyphi was undertaken from June 2017 to June 2022. A total of 4051 blood samples were collected from patients attending inpatient and outpatient departments of the School of Tropical Medicine (Kolkata, India) hospital. Blood samples were cultured, and culture positive samples were further p

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Faik, Ashna J., Ali Hussain Al-wardy, and Raghad Abdul Elah Mohammad. "Multiplex PCR for Identification of Salmonella enterica serovars Typhi and Paratyphi A by Selective Amplification of tyv, prt, viaB, fliC-d and fliC-a Genes." Journal of Biotechnology Research Center 8, no. 2 (2014): 60–65. http://dx.doi.org/10.24126/jobrc.2014.8.2.333.

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Salmonellosis is responsible for large number of infections in both human and animals. Salmonella enterica serovar Typhi is a causative agent of typhoid fever and Salmonella enterica serovar Paratyphi A is a causative agent of paratyphoid fever. Conventional methods of isolation of Salmonella strains take 4-6 days to complete and are therefore laborious and require substiantial manpower. Therefore development of a PCR assay that can target multiple genes for rapid detection of S. Typhi and S.Paratyphi A. Methods: Synthetic primers for O, H, and Vi antigen genes, tyv , prt , fliC-d, fliC-a, and

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Napoleoni, Maira, Stefano Ceschia, Elisa Mitri, et al. "Identification of Salmonella Serogroups and Distinction Between Typhoidal and Non-Typhoidal Salmonella Based on ATR-FTIR Spectroscopy." Microorganisms 12, no. 11 (2024): 2318. http://dx.doi.org/10.3390/microorganisms12112318.

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Salmonellosis is the second-most commonly reported foodborne gastrointestinal infection in the European Union and a major contributor to foodborne outbreaks globally. Salmonella serotyping differentiates typhoidal strains requiring antibiotic therapy (e.g., serovars Typhi, Paratyphi A, Paratyphi B-d-tartrate negative, Paratyphi C) from typically self-limiting non-typhoidal Salmonella (NTS) strains, making precise identification essential for appropriate treatment and epidemiological tracking. At the same time, the ability to identify the serogroup of Salmonella, regardless of which of the abov

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Zahra, Fatima Tuz, Sidrah Saleem, Muhammad Imran, Nida Javed, Ayesha ,. Ghazal, and Saadia Ch. "Association of Drinking Water and Enteric Fever: A Disguised Source of Infection." Pakistan Journal of Medical and Health Sciences 16, no. 5 (2022): 41–44. http://dx.doi.org/10.53350/pjmhs2216541.

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Background: Enteric fever is an illness caused by Salmonella enterica serovars Typhi and Paratyphi A and B. The mode of transmission is the ingestion of contaminated food and water. Aim: To check that whether drinking water is the source of infection or not. Methodology: 202 water samples were collected from the various areas of Lahore. 120 samples were taken from houses and the neighbourhood of confirmed typhoid patients and 82 samples were randomly collected. The samples were centrifuged, enriched and then subcultured on XLD media. The isolated colonies were identified by biochemical reactio

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Kunwar, Dhirendra, Sabita Bhatta, Raina Chaudhary, and Komal Raj Rijal. "Antibiotic Susceptibility Pattern of Nalidixic Acid Resistant Salmonella Isolates in Shree Birendra Hospital Chhauni." Tribhuvan University Journal of Microbiology 4 (November 16, 2018): 11–14. http://dx.doi.org/10.3126/tujm.v4i0.21669.

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Objectives: This study was aimed to know the prevalence of Nalidixic acid resistant Salmonella isolates and their antibiotic susceptibility pattern.Methods: A total of 4619 febrile patients suspecting the cases of typhoid fever by clinician, attending at Shree Birendra hospital during May- November 2013 were subjected to culture. Blood sample (5ml) was collected from the suspected cases and inoculated immediately into 45ml of Brain heart infusion broth (BHI) and further processed for the identification of Salmonella Typhi and S. Paratyphi. Antimicrobial susceptibility pattern of S. Typhi and S

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Saad, Maliha, Humaira Khan, Sabahat Rehman, Humera Javed, Nazish Babar, and Sara Najeeb. "Susceptibility to Fluoroquinolones among Salmonella Enterica Serovars in Blood Culture." Pakistan Journal of Medical and Health Sciences 16, no. 9 (2022): 847–49. http://dx.doi.org/10.53350/pjmhs22169847.

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Background and Aim: Enteric fever is a severe bloodstream infection contributing to higher rate of morbidity and mortality around the globe caused by S. Typhi and Paratyphi A. Fecal-oral route is the prevalent transmission route of enteric fever that manifests with various clinical outcomes such as nausea, fever, transient rash, chills, hepatosplenomegaly, abdominal discomfort, and malaise. Assessment of susceptibility to fluoroquinolones in Salmonella enterica serovars in blood cultures was the prime objective of the current study. Methodology: This cross-sectional study was carried out on 32

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Rödel, Jürgen, Birgit Edel, Sascha D. Braun, et al. "Simple differentiation of Salmonella Typhi, Paratyphi and Choleraesuis from Salmonella species using the eazyplex TyphiTyper LAMP assay." Journal of Medical Microbiology 69, no. 6 (2020): 817–23. http://dx.doi.org/10.1099/jmm.0.001201.

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Introduction. Identification of typhoidal Salmonella (TS) serovars and their discrimination from non-typhoidal Salmonella (NTS) is conventionally performed by seroagglutination. This method is labour-intensive, requires technical experience and can be inconclusive in some cases. Molecular assays may be reliable alternative diagnostic tools. Aim. This study was designed to evaluate the eazyplex TyphiTyper based on loop-mediated isothermal amplification (LAMP) for fast identification of TS and S. Choleraesuis in culture. Methodology. A total of 121 Salmonella strains and 33 isolates of other Ent

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H. Shaikh, Parvez, Mishra J. K., Jayshree A.Pohekar, Ganesh Maher, Manjushree V. Mulay, and Wyawahare A.S. "PREVALENCE AND ANTIBIOGRAM OF SALMONELLA SPECIES ISOLATED FROM BLOOD SPECIMEN AT A TERTIARY CARE HOSPITAL." International Journal of Advanced Research 13, no. 03 (2025): 450–56. https://doi.org/10.21474/ijar01/20587.

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Background: This retrospective study aimed to evaluate the prevalence and antimicrobial susceptibility patterns of Salmonella species isolated from blood specimens at a tertiary care hospital between September 2022 and September 2024. Methods: Blood culture was done by automated system (BacT/ALERT, Biomerieux). Identification, antibiotic susceptibility testing and MIC value were done with the help of Vitek-2 (Biomerieux System). AST is also done by Conventional method (Kirby-Bauers Disk diffusion) for some Antibiotics like Ampicillin, Azithromycin (only for S. Typhi) and Chloramphenicol. To co

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Ferreira, Cristina Motta, Ana Galdina dos Reis Mendes, Guilherme Motta Antunes Ferreira, et al. "Whole genomic sequence of Salmonella Paratyphi B isolated from a hematology patient in the northern region of Brazil." Peer Review 6, no. 8 (2024): 155–69. http://dx.doi.org/10.53660/prw-2084-3820.

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Salmonella Typhi is a zoonotic pathogen responsible for a life-threatening bloodstream infection that is common in places with limited sanitation and hygiene. This study aimed to characterize the resistance genes and sequence type (ST) of Salmonella Paratyphi isolated from a patient with acute myeloid leukemia (AML) undergoing treatment at Fundação HEMOAM, in the north of Brazil. Phenotypic identification and antimicrobial susceptibility testing were performed using the VITEK-2 system. The genomic DNA was sequenced in Illumina® equipment. Resistance genes were identified through the Genome Ann

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Uddin, Muhammad Nazir, Alveena Mukhtiar, Muddasir Khan, Ghadir Ali, Wajid Khan, and Taj Uddin. "Antibiogram Analysis of Salmonella paratyphi A Isolated from Gall Bladder Patients in District Peshawar, Pakistan." BioScientific Review 4, no. 4 (2022): 57–72. http://dx.doi.org/10.32350/bsr.44.03.

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Salmonella paratyphi A harbors gall bladder in the human body. It serves as a site of persistence for Salmonella paratyphi A. It is an enteric pathogen which has become resistant to many drugs. Therefore, the current study was designed for the identification and antibiogram analysis of S. paratyphi A, isolated from the gall bladder patients undergone cholecystectomy. It included 250 samples of bile, stone, and tissue of patients. The samples were cultured on blood, macConkey, and Salmonella Shigella media. Further identification was carried out by morphological oxidase test and Analytical Prof

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Killmann, Helmut, Christina Herrmann, Helga Wolff, and Volkmar Braun. "Identification of a New Site for Ferrichrome Transport by Comparison of the FhuA Proteins of Escherichia coli,Salmonella paratyphi B, Salmonella typhimurium, and Pantoea agglomerans." Journal of Bacteriology 180, no. 15 (1998): 3845–52. http://dx.doi.org/10.1128/jb.180.15.3845-3852.1998.

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ABSTRACT The fhuA genes of Salmonella paratyphi B,Salmonella typhimurium, and Pantoea agglomeranswere sequenced and compared with the known fhuA sequence ofEscherichia coli. The highly similar FhuA proteins displayed the largest difference in the predicted gating loop, which inE. coli controls the permeability of the FhuA channel and serves as the principal binding site for the phages T1, T5, and φ80. All the FhuA proteins contained the region in the gating loops required in E. coli for ferrichrome and albomycin transport. The three subdomains required for phage binding were contained in the g

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Omotola, Justina. "Antibiotic Resistance of Typhoidal Salmonella Isolated from Humans in Kaduna and its Associated Factors." Open Access Journal of Microbiology & Biotechnology 6, no. 1 (2021): 1–7. http://dx.doi.org/10.23880/oajmb-16000181.

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Background: Pathogenic Salmonella species cause various types of infections ranging from mild gastroenteritis to life- threatening typhoid fever caused by Salmonella typhi , or paratyphoid fever caused by S. paratyphi A, B or C; of which S . paratyphi A is prevalent. Currently, treatment of typhoid infection is being hampered by the emergence of multidrug-resistant strains, while vaccines provide only temporal or partial protection. Objective: The aim of the study was to determine antibiotic resistance of typhoid causing Salmonella isolated from human stool. Methodology: A cross-sectional desc

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Sudhaharan, Sukanya, Kanne Padmaja, Rachana Solanki, Vemu Lakshmi, Pamidi Umabala, and Bhaskara Aparna. "Extra-intestinal salmonellosis in a tertiary care center in South India." Journal of Infection in Developing Countries 8, no. 07 (2014): 831–37. http://dx.doi.org/10.3855/jidc.3731.

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Introduction: The present study is a retrospective analysis of a total of 36 cases of bacteriologically proven extra-intestinal salmonellosis, managed at Nizam’s Institute of Medical Sciences, between 1987 and 2012 (25 years). The extra-intestinal sites involved were the skin, cerebrum, spleen, ovary, synovium, and the skeletal muscle. Methodology: The extra-intestinal specimens were first processed using standard methods. Colonies suspected as Salmonella were identified by standard laboratory methods, initially by manual biochemical reactions and later by the API system (bioMerieux, Marcy l’E

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Akhtar, Rokshana, Saifa Kismat, Fatima Afroz, Sanjida Khondakar Setu, Shaheda Anwar, and Sharmeen Ahmed. "Antimicrobial susceptibility pattern of multidrug resistant typhoidal Salmonella isolates at Bangabandhu Sheikh Mujib Medical University." Bangladesh Journal of Medical Microbiology 15, no. 2 (2021): 19–22. http://dx.doi.org/10.3329/bjmm.v15i2.57816.

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Typhoid fever is a major public health problem in the developing world caused by Salmonella Typhi and Salmonella Paratyphi A. Multidrug resistant (MDR) Salmonella has emerged as a cause of concern. This study was done to evaluate status in antimicrobial susceptibility pattern of Salmonella Typhi and Salmonella Paratyphi A from blood culture isolates in Bangabandhu Sheikh Mujib Medical University during the period of September 2018 to August 2019. Suspected Salmonella spp. was collected from blood culture samples and identification was done by conventional microbiological procedure. Antimicrobi

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Alam, Mohammad Murshid, Lillian L. Tsai, Sean M. Rollins, et al. "Identification ofIn Vivo-Induced Bacterial Proteins during Human Infection with Salmonella enterica Serotype Paratyphi A." Clinical and Vaccine Immunology 20, no. 5 (2013): 712–19. http://dx.doi.org/10.1128/cvi.00054-13.

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ABSTRACTSalmonella entericaserotype Paratyphi A is a human-restricted pathogen and the cause of paratyphoid A fever. Using a high-throughput immunoscreening technique,in vivo-induced antigen technology (IVIAT), we identified 20 immunogenic bacterial proteins expressed in humans who were bacteremic withS. Paratyphi A but not those expressed inS. Paratyphi A grown under standard laboratory conditions. The majority of these proteins have known or potential roles in the pathogenesis ofS. enterica. These include proteins implicated in cell adhesion, fimbrial structure, adaptation to atypical condit

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MOHANTY, S., K. RENUKA, S. SOOD, B. K. DAS, and A. KAPIL. "Antibiogram pattern and seasonality of Salmonella serotypes in a North Indian tertiary care hospital." Epidemiology and Infection 134, no. 5 (2006): 961–66. http://dx.doi.org/10.1017/s0950268805005844.

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The antibiogram pattern and seasonal distribution of Salmonella serotypes were analysed retrospectively over a 6-year period from January 1999 to December 2004. Blood cultures received in the Bacteriology Laboratory were processed by standard procedures and the Salmonella spp. isolates were identified with specific antisera and standard biochemical tests. Antimicrobial susceptibility testing was carried out by a standard disc diffusion method and the minimum inhibitory concentration (MIC) of ciprofloxacin for 332 representative Salmonella isolates was determined by E test. Salmonella Typhi (75

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Rodríguez-Hernández, Roy, Johan F. Bernal, Jimmy F. Cifuentes, et al. "Prevalence and Molecular Characterization of Salmonella Isolated from Broiler Farms at the Tolima Region—Colombia." Animals 11, no. 4 (2021): 970. http://dx.doi.org/10.3390/ani11040970.

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Salmonella is an important animal and human pathogen responsible for Salmonellosis, and it is frequently associated with the consumption of contaminated poultry products. The aim of this study was to estimate the prevalence of Salmonella in the poultry farms and to determine the genetic relationship. A total of 135 samples collected from fifteen broiler farms, including cloacal, feed, water, environmental and farm operator faeces samples were subjected to microbiological isolation. Molecular confirmation of Salmonella isolates was carried out by amplification of the invA gene, discrimination o

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Huda, Noor ul, Muhammad Sohail, Zulfiqar Ali Mirani, Qurat ul Ain Hyder, and Saeed Khan. "Source tracking of extensively drug resistant Salmonella Typhi in food and raw vegetables using molecular approaches." Journal of Infection in Developing Countries 18, no. 02 (2024): 227–34. http://dx.doi.org/10.3855/jidc.18289.

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Introduction: Extensively drug resistant (XDR) strains of the Salmonella lineages have been reported to spread from Africa to South Asia. XDR strains are resistant to fluoroquinolones, chloramphenicol, co-trimoxazole, and ampicillin, resulting in treatment failure. The objectives of this study included the investigation of transmission of S. Typhi lineages and the identification of the potentially contaminated sources of the XDR typhoid outbreak from different urban areas by using molecular techniques. Methodology: Environmental samples, including food samples, were collected from different to

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Sajib, Mohammad S. I., Arif M. Tanmoy, Yogesh Hooda, et al. "Trends in antimicrobial resistance amongst Salmonella Paratyphi A isolates in Bangladesh: 1999–2021." PLOS Neglected Tropical Diseases 17, no. 11 (2023): e0011723. http://dx.doi.org/10.1371/journal.pntd.0011723.

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Background Typhoid and paratyphoid remain common bloodstream infections in areas with suboptimal water and sanitation infrastructure. Paratyphoid, caused by Salmonella Paratyphi A, is less prevalent than typhoid and its antimicrobial resistance (AMR) trends are less documented. Empirical treatment for paratyphoid is commonly based on the knowledge of susceptibility of Salmonella Typhi, which causes typhoid. Hence, with rising drug resistance in Salmonella Typhi, last-line antibiotics like ceftriaxone and azithromycin are prescribed for both typhoid and paratyphoid. However, unlike for typhoid,

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Taneja, Juhi. "Antimicrobial Resistance in Typhoidal Salmonellain a Tertiary Care Teaching Centre in North India." Journal of Communicable Diseases 53, no. 1 (2021): 1–4. http://dx.doi.org/10.24321/0019.5138.202101.

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Introduction: Enteric fever continues to carry a high burden of morbidity and mortality in India. There have been reports of emergence of ceftriaxone resistant typhoidal Salmonella from Asia. Monitoring of antimicrobial resistance trends in typhoidal Salmonella is crucial to support in clinical decision making. Aim: To study the current susceptibility pattern of typhoidal salmonella isolates in our setup. Methods: This retrospective study was conducted on 144 non-repeat blood-culture isolates of S. Typhi, and S. Paratyphi A obtained from 3926 blood cultures received at 510-bedded tertiary-care

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Amare, Azanaw, Fekadu Asnakew, Yonas Asressie, et al. "Prevalence of multidrug resistance Salmonella species isolated from clinical specimens at University of Gondar comprehensive specialized hospital Northwest Ethiopia: A retrospective study." PLOS ONE 19, no. 5 (2024): e0301697. http://dx.doi.org/10.1371/journal.pone.0301697.

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Background Multidrug resistance Salmonellosis remains an important public health problem globally. The disease is among the leading causes of morbidity and mortality in developing countries, but there have been limited recent studies about the prevalence, antimicrobial resistance, and multidrug resistance patterns of Salmonella isolates from various clinical specimens. Objective Aimed to assess the prevalence, antimicrobial resistance, and multidrug resistance patterns of Salmonella isolates from clinical specimens at the University of Gondar Comprehensive Specialised Hospital, northwestern Et

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Baker, Stephen, Kathryn E. Holt, Archie C. A. Clements, et al. "Combined high-resolution genotyping and geospatial analysis reveals modes of endemic urban typhoid fever transmission." Open Biology 1, no. 2 (2011): 110008. http://dx.doi.org/10.1098/rsob.110008.

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Typhoid is a systemic infection caused by Salmonella Typhi and Salmonella Paratyphi A, human-restricted bacteria that are transmitted faeco-orally. Salmonella Typhi and S . Paratyphi A are clonal, and their limited genetic diversity has precluded the identification of long-term transmission networks in areas with a high disease burden. To improve our understanding of typhoid transmission we have taken a novel approach, performing a longitudinal spatial case–control study for typhoid in Nepal, combining single-nucleotide polymorphism genotyping and case localization via global positioning. We s

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Cheah, Yoke−Kqueen, Learn−Han Lee, Cheng−Yun Catherine Chieng, and Vui−Ling Clemente Michael Wong. "Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites." Polish Polar Research 36, no. 1 (2015): 67–78. http://dx.doi.org/10.1515/popore-2015-0001.

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Abstract This project aimed to isolate and characterize volcanic soil Actinobacteria from Deception Island, Antarctic. A total of twenty−four Actinobacteria strains were isolated using four different isolation media (Starch casein agar, R2 agar, Actinomycete isolation agar, Streptomyces agar) and characterized basing on 16S rRNA gene sequences. Tests for second− arymetabolites were performed using well diffusion method to detect antimicrobial activities against eight different pathogens, namely Staphyloccocus aureus ATCC 33591, Bacillus megaterium, Enterobacter cloacae, Klebsiella oxytoca, S.

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LAN, TRAN T. Q., MARIE-LOU GAUCHER, NGUYEN T. M. NHAN, ANN LETELLIER, and SYLVAIN QUESSY. "Distribution of Virulence Genes among Salmonella Serotypes Isolated from Pigs in Southern Vietnam." Journal of Food Protection 81, no. 9 (2018): 1459–66. http://dx.doi.org/10.4315/0362-028x.jfp-17-408.

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ABSTRACT This study was carried out to evaluate the prevalence of Salmonella serogroups and serotypes and their virulence gene carriage in pig fecal samples from farms and slaughterhouses in some southern provinces of Vietnam. The presence of Salmonella was assessed based on culture enrichment of the collected samples and biochemical and serological analyses; 27.7% (51) of 184 samples were posititve for Salmonella. Based on the availability of antisera, serogroups were determined for 61% (31) of 51 isolates. Twenty isolates belonging to Salmonella serotypes Typhimurium (10 isolates), Anatum (8

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Nov, Vandarith, Darapheak Chau, and Chhorvann Chhea. "1439. High Rates of Drug Resistance in Escherichia coli from a Pilot Antimicrobial Resistance Surveillance System in Cambodia." Open Forum Infectious Diseases 7, Supplement_1 (2020): S723—S724. http://dx.doi.org/10.1093/ofid/ofaa439.1620.

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Abstract Background Antimicrobial resistance (AMR) is a major and growing global public health problem. The Cambodia Ministry of Health established a pilot laboratory-based AMR surveillance system for blood specimens in 2017. The objective of this study is to characterize AMR among pathogenic isolates from blood samples. Methods A retrospective analysis was performed using one year of data from a pilot AMR Surveillance system in Cambodia. Four blood culture isolate pathogens were included: Escherichia coli, Klebsiella pneumoniae, Salmonella Typhi /Salmonella Paratyphi A and Staphylococcus aure

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Makkar, Anuradha, Shilpi Gupta, Inam Danish Khan, et al. "Epidemiological Profile and Antimicrobial Resistance Pattern of Enteric Fever in a Tertiary Care Hospital of North India – a Seven Year Ambispective Study." Acta Medica (Hradec Kralove, Czech Republic) 61, no. 4 (2018): 125–30. http://dx.doi.org/10.14712/18059694.2018.130.

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Introduction: Enteric-fever is a major public-health problem in developing countries emerging as multidrug-resistant, Nalidixic-acid resistant and extremely drug-resistant Salmonella (Pakistan, 2016), has intensified the use of WHO watch/reserve group antimicrobials such as azithromycin and meropenem. Methods: This ambispective-study was conducted on 782 non-repeat blood-culture isolates of S. Typhi, S. Paratyphi A and S. Paratyphi B obtained from 29,184 blood cultures received at a 1000-bedded tertiary-care hospital of North-India from 2011–2017. Identification and antibiograms were obtained

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Al-Muhannak, Fadhil H. N., Hashim Ali Abdualmeer Al-sherees, Ibrahim Abed Ali Abdul Sada, Thikra Abdullah Mahmood, and Rasha Fadhel Obaid. "Detecting Phenotypic and Genotypic of the Antibiotic Resistant Salmonella enterica Serotype Paratyphi Isolated from Blood Samples in Najaf Province /Iraq." Kufa Medical Journal 18, no. 2 (2021): 98–106. http://dx.doi.org/10.36330/kmj.v18i2.10292.

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Background & Objectives: Salmonella Paratyphi is a leading cause of human paratyphoid fever in developing countries, causing deaths in humans worldwide. There are several paths for catching paratyphoid fever, but the close contact between patients and healthy humans is so far the most frequent cause of human infections. No study is found about the antibiotic resistance (phenotypic and genetic) of S. Paratyphi isolated from patients in Al-Najaf Province/Iraq, to the researcher’s best knowledge. Hence, this study aimed to determine the prevalence of S. Paratyphi isolates from blood specimens

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Hossain, Md Mobarok, Tasnim Jabin, Md Ifrat Hossain, et al. "Antibiotic resistance profiling of clinical isolates of Salmonella enterica Serovar Paratyphi A in Dhaka, Bangladesh." Stamford Journal of Microbiology 11, no. 1 (2021): 14–16. http://dx.doi.org/10.3329/sjm.v11i1.57146.

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The study was conducted to detect the antibiotic resistance profile of the clinical isolates of Salmonella enterica Serovar Paratyphi A from 100 blood samples of patients from different age groups suspected to be suffering from enteric fever. The pure cultures of the bacterial isolates were collected from some renowned diagnostic centers of Dhaka and they were further characterized through the conventional culture, microscopy and biochemical examinations. These isolates were cross checked for the antibiogram profile by the Kirby-Bauer disc diffusion method against ten different types of antibi

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Chand, Hari Jung, Komal Raj Rijal, Biswas Neupane, Vijay Kumar Sharma, and Bharat Jha. "Re-emergence of susceptibility to conventional first line drugs in Salmonella isolates from enteric fever patients in Nepal." Journal of Infection in Developing Countries 8, no. 11 (2014): 1483–87. http://dx.doi.org/10.3855/jidc.4228.

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Introduction: Enteric fever is endemic in Nepal and poses a significant public health burden. The first-line drugs ampicillin, chloramphenicol, and cotrimoxazole have not been part of empirical therapy for two decades due to the development of multidrug-resistant Salmonella strains. The objective of this study was to determine the antibiogram pattern of Salmonella serovars isolated from the blood of clinically suspected enteric fever patients. Methodology: A cross sectional study was carried out in a tertiary care hospital in Lalitpur, Nepal, between July 2011 and February 2012. Standard micro

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Soltan Dallal, Mohammad Mehdi, Milad Abdi, Mahya Khalilian, et al. "Isolation, Identification, and Antibiotic Susceptibility Testing of Salmonella Isolated from Foodborne Outbreaks." International Journal of Enteric Pathogens 8, no. 3 (2020): 80–83. http://dx.doi.org/10.34172/ijep.2020.18.

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Background: Foodborne diseases are a major problem worldwide. The epidemiological investigations in many parts of the world have shown an increase in infections caused by Salmonella serovars. Furthermore, the emergence of drug resistance among them has become a major global concern and awareness of the resistance patterns of Salmonella could be very useful in treatment of diseases. Objective: This study aimed to investigate Salmonella serotypes in foodborne outbreaks by sequencing of ITS region of 16S-23SrRNA gene and to determine their antimicrobial susceptibility pattern. Materials and Metho

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Raharjo, D., R. Yulistiani, W. Setyarini, R. Y. Arizandy, W. Prayoga, and T. Shirakawa. "Rapid identification of Salmonella enterica serovars Typhi and Salmonella enterica serovars Paratyphi A from chicken meat." IOP Conference Series: Materials Science and Engineering 633 (October 8, 2019): 012003. http://dx.doi.org/10.1088/1757-899x/633/1/012003.

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