Academic literature on the topic 'Biochemical research methods'

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Journal articles on the topic "Biochemical research methods"

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Edelev, N. S., V. G. Vorob'ev, and I. S. Edelev. "Application of biochemical research methods for solving questions of forensic medical practice." Sudebno-meditsinskaya ekspertiza 62, no. 4 (2019): 63. http://dx.doi.org/10.17116/sudmed20196204163.

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Gureeva, N. V. "River water quality research within the city limits using some biochemical methods." Contemporary Problems of Ecology 4, no. 1 (2011): 84–90. http://dx.doi.org/10.1134/s1995425511010140.

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K, Hema Shree. "Saliva Collection Methods: A Comprehensive Guide for Scientific Research." International Journal of Orofacial Biology 7, no. 2 (2023): 21–25. http://dx.doi.org/10.56501/intjorofacbiol.v7i2.1058.

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Saliva has recently emerged as a valuable biological fluid in scientific research due to its rich biochemical composition and non-invasive accessibility. This article aims to provide a comprehensive guide to saliva collection methods in scientific research, covering procedures, materials, contamination prevention, storage protocols, collection methods, clinical trial considerations, and sample stabilization. By adhering to meticulous protocols, researchers can ensure the accuracy and reliability of their findings, thus maximizing the potential of saliva as a biospecimen for various research ap
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Aguilar, Adriana, Jacques Planas, Enrique Trilla, and Juan Morote. "Methods for Evaluating the Efficacy of Medical Castration: A Systematic Review." Cancers 15, no. 13 (2023): 3479. http://dx.doi.org/10.3390/cancers15133479.

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Measuring serum testosterone determination during medical castration is recommended by prostate cancer (PCa) guidelines to assess its efficacy and define castration resistance. It has been suggested that other biochemical compounds, such as free testosterone or luteinising hormone (LH), could also assess castration efficacy. We aimed to analyse the current evidence for serum biochemical compounds that could be appropriate candidates for evaluating medical castration efficacy. A systematic review was conducted after two investigators independently searched the literature in the PubMed, Cochrane
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Murskiy, S. I. "On possibility of using routine biochemical techniques for research of alternative biological human liquids." Medical alphabet 4, no. 35 (2020): 54–57. http://dx.doi.org/10.33667/2078-5631-2019-4-35(410)-54-57.

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Biochemical studies of alternative human biological fluids are not common in laboratory practice due to the lack of validation methods. The aim of the study was to validatе on semen plasma routine biochemical research methods using an automatic biochemical analyzer. The studies were carried out on a Cobas Integra 400 plus automatic biochemical analyzer (Roche, Switzerland) using original Roche reagents (Switzerland). Materials for the study were 30 samples of semen plasma obtained from clinically healthy men of reproductive age. Planning and organization of validation activities were carried o
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Lane, Dan. "Expect the unexpected: presenting research leads to unforeseen gains." Biochemist 41, no. 4 (2019): 48–49. http://dx.doi.org/10.1042/bio04104048.

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Dan Lane is a first year PhD student at the University of Leicester, developing biochemical methods in non-adherence testing alongside Leicester Diabetes Centre. A pilot study he helped to conduct prior to the start of his postgraduate degree was accepted by Diabetes UK and so, with the help of a travel grant from the Biochemical Society, he travelled to Liverpool to present his work. The Biochemical Society provides General Travel Grants to support attendance at scientific meetings, training events or any other event relevant to your career. Postgraduate, Early Career, Full and Emeritus Membe
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Feng, Hao. "Research Progress in Pharmaceutical Wastewater Treatment Technology." E3S Web of Conferences 118 (2019): 04019. http://dx.doi.org/10.1051/e3sconf/201911804019.

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With the development of the medical industry, according to the characteristics of pharmaceutical wastewater, the corresponding pharmaceutical wastewater treatment technology is used to optimize the technology in the production process, which is a measure for the welfare of human health. The wastewater discharged during the production process, especially the pharmaceutical wastewater, is very polluting to the human environment. According to the physicochemical and biochemical and combined process technologies used in pharmaceutical wastewater at home and abroad, new methods are used to improve
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Vranová, Valerie, Theodore Danso Marfo, and Klement Rejšek. "Soil Scientific Research Methods Used in Archaeology – Promising Soil Biochemistry: a Mini-review." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 63, no. 4 (2015): 1417–26. http://dx.doi.org/10.11118/actaun201563041417.

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This work seeks to review soil scientific methods that have been used and are still being used in archaeology. This review paper aims at emphasising the importance of soil science practice to archaeology thus adding a scientific analytical nature to the cultural nature of archaeology. Common methods (physical, chemical and biochemical) used to analyse archaeological soils and artefacts is touched on and their strengths and shortcomings duly noted to become the base for future research. Furthermore, the authors made emphasis on distinctive excavating/sampling methods, biochemical analyses focus
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Zhu, Ziyue. "Current common prodrug delivery methods and biochemical property." Theoretical and Natural Science 32, no. 1 (2024): 67–71. http://dx.doi.org/10.54254/2753-8818/32/20240798.

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Prodrugs are pivotal in overcoming drug delivery and absorption challenges. These bioreversible derivatives are strategically designed to address medicines poor bioavailability and administration constraints. Prodrugs offer versatile solutions for various delivery methods, including oral, dermal, nasal, and central nervous system (CNS) routes and tumor targeting. This literature review aims to summarize several prodrugs delivery methods. The report also focuses on the common concepts of prodrug design. The utilization of prodrugs also includes innovative strategies, such as receptor-mediated i
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Maharani, Ni Putu Radela, Nur Ashfiya Fadlyah, Dyah Ayu Woro Setyaningrum, and Nany Hairunisa. "Effects of Euthanasia on Animal Research." Veterinary Biomedical and Clinical Journal 6, no. 1 (2024): 41–46. http://dx.doi.org/10.21776/ub.vetbioclinj.2024.006.01.6.

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The use of experimental animals is widely conducted in the medical field, both for research on the processes of disease occurrence (pathogenesis), the causes of disease (etiology), and research on therapy. Therefore, it is important to understand how euthanasia in experimental animals affects the integrity of the sample and its influence on research results. This article was compiled from several literature sources searched from Google Schoolar and PubMed published within the last 10 years, with keywords such as "code of ethics in experimental animals", "methods of euthanasia in experimental a
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Dissertations / Theses on the topic "Biochemical research methods"

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Brown, John Ellsworth III. "Kernel Methods in Biochemical Informatics and Applications to DNA Repair Research." 京都大学 (Kyoto University), 2010. http://hdl.handle.net/2433/120374.

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Barve, Aabha. "Development of an Optical Method for the Detection of Homocysteine as a Disease Biomarker Using Fluorescein-Aldehydes." PDXScholar, 2015. https://pdxscholar.library.pdx.edu/open_access_etds/2221.

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Homocysteine is a natural occurring aminothiol. It is an intermediate product in the metabolism of methionine. Methionine is an essential amino acid required for protein synthesis. Metabolic irregularities disrupt homocysteine levels in plasma. Elevated homocysteine levels are directly linked to folate and cobalamin (vitamin B12) deficiencies, and are an independent risk factor for cardiovascular diseases. High homocysteine levels have also been associated with Alzheimer's, osteoporosis, renal failure, cancer, birth defects and pregnancy complications. The association of elevated homocysteine
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"Quantitative peptidomic profiling with the use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry: method development and application in cancer detection." 2004. http://library.cuhk.edu.hk/record=b5896178.

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Kong Kam-chuen, Ebenezer.<br>Thesis submitted in: July 2003.<br>Thesis (M.Phil.)--Chinese University of Hong Kong, 2004.<br>Includes bibliographical references (leaves 131-149).<br>Abstracts in English and Chinese.<br>Abstract in English --- p.i<br>Abstract in Chinese --- p.ii<br>Table of Content --- p.iii -vii<br>Acknowledgement --- p.viii<br>List of Abbreviations --- p.ix -x<br>List of Tables --- p.xi<br>List of Figures --- p.xii -xiii<br>List of Appendices --- p.xiv -xv<br>Chapter CHAPTER 1 --- Review of Literature<br>Chapter 1.1 --- Peptidomic Research<br>Chapter 1.1.1 --- Proteomi
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Reger, Michael Kent. "Dietary intake and urinary excretion of phytoestrogens in relation to cancer and cardiovascular disease." Thesis, 2014. http://hdl.handle.net/1805/6053.

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Indiana University-Purdue University Indianapolis (IUPUI)<br>Phytoestrogens that abound in soy products, legumes, and chickpeas can induce biologic responses in animals and humans due to structural similarity to 17β-estradiol. Although experimental studies suggest that phytoestrogen intake may alter the risk of cancer and cardiovascular disease, few epidemiologic studies have investigated this research question. This dissertation investigated the associations of intake of total and individual phytoestrogens and their urinary biomarkers with these chronic conditions using data previously collec
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Books on the topic "Biochemical research methods"

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1946-, Snell Keith, and Mullock B, eds. Biochemical toxicology: A practical approach. IRL Press, 1987.

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Z, Litten Raye, Allen John P, and National Institute on Alcohol Abuse and Alcoholism (U.S.), eds. Measuring alcohol consumption: Psychosocial and biochemical methods. Humana Press, 1992.

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Fernandez-Botran, Rafael. Methods in cellular immunology. 2nd ed. CRC Press, 2001.

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Workshop on Computation of Biochemical Pathways and Genetic Networks (3rd 2003 Heidelberg, Germany). 3rd Workshop on Computation of Biochemical Pathways and Genetic Networks : EML Research, Villa Bosch, Heidelberg, October 6-7, 2003. Edited by Gauges R. Logos, 2003.

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Vengrova, Sonya, and Jacob Z. Dalgaard. DNA replication: Methods and protocols. Humana Press, 2015.

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Dennis, Cosmatos, and Chow Shein-Chung 1955-, eds. Translational medicine: Strategies and statistical methods. Chapman & Hall/CRC, 2009.

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S, Bartlett John M., ed. Ovarian cancer: Methods and protocols. Humana Press, 2001.

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1951-, Rossbach M., Institut "Jožef Stefan", and Kernforschungsanlage Jülich, eds. Reference materials and methods in environmental and biochemical research: Report on a bilateral cooperation between the "Jozef Stefan" Institute, Ljubljana and the KFA Jülich. Forschungszentrum Jülich, 1992.

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Workshop on Computation of Biochemical Pathways and Genetic Networks (4th 2005 Heidelberg, Germany). 4th Workshop on Computation of Biochemical Pathways and Genetic Networks: A BioSim event : EML research, Villa Bosch, Heidelberg, September 12-13, 2005. Edited by Kummer U. (Ursula) and EML Research (Firm). Logos, 2005.

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Feng, Wang, ed. Biomarker methods in drug discovery and development. Humana Press, 2008.

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Book chapters on the topic "Biochemical research methods"

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Herrmann, Markus. "Methods in Bone Biology in Animals: Biochemical Markers." In Osteoporosis Research. Springer London, 2011. http://dx.doi.org/10.1007/978-0-85729-293-3_6.

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Fedoroff, Nina. "Biochemical and Molecular Techniques in Maize Research." In Genetic Engineering: Principles and Methods. Springer US, 1985. http://dx.doi.org/10.1007/978-1-4684-4973-0_5.

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Wu, Yaping, and Kunshan Gao. "Biochemical Inhibitors for Algae." In Research Methods of Environmental Physiology in Aquatic Sciences. Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-5354-7_29.

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Das, Bidisha, Joy Chakraborty, and Krishnananda Chattopadhyay. "Emerging Techniques in Cellular and Biomolecular Research." In Biochemical and Biophysical Methods in Molecular and Cellular Biology. Springer Nature Singapore, 2025. https://doi.org/10.1007/978-981-96-2088-3_1.

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Crépin, Laurent, Fabrice Harrouet, Sébastien Kerdélo, Jacques Tisseau, and Pascal Redou. "Computational Methods for the Parallel 3D Simulation of Biochemical Kinetics at the Microscopic Scale." In Bioinformatics Research and Applications. Springer Berlin Heidelberg, 2013. http://dx.doi.org/10.1007/978-3-642-38036-5_7.

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Turk, Cebrahil. "Use of Some Bee Products in the Aquaculture Industry." In Methods of Biochemical Analysis of Bee Products. Nobel Tip Kitabevleri, 2024. http://dx.doi.org/10.69860/nobel.9786053359326.9.

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In addition to their traditional medicinal uses, bee products have also had an important place in the aquaculture industry in recent years. Bee products such as honey, propolis, royal jelly and bee venom offer potential benefits for a variety of applications in aquaculture. These products are used to improve fish health, combat diseases, increase growth rate and support environmental sustainability. For example, the antimicrobial properties of propolis can be effective in treating and preventing fish diseases, while the nutritional components of honey can promote the development of aquaculture
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Rinne-Garmston, Katja T., Gerhard Helle, Marco M. Lehmann, Elina Sahlstedt, Jürgen Schleucher, and John S. Waterhouse. "Newer Developments in Tree-Ring Stable Isotope Methods." In Stable Isotopes in Tree Rings. Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-92698-4_7.

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AbstractThe tree-ring stable C, O and H isotope compositions have proven valuable for examining past changes in the environment and predicting forest responses to environmental change. However, we have not yet recovered the full potential of this archive, partly due to a lack understanding of fractionation processes resulting from methodological constraints. With better understanding of the biochemical and tree physiological processes that lead to differences between the isotopic compositions of primary photosynthates and the isotopic compositions of substrates deposited in stem xylem, more re
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Stöger, Benjamin, and Claudia Nerdel. "Using External Representations to Support Mathematical Modelling Competence in Biology Education." In Shaping the Future of Biological Education Research. Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-44792-1_3.

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AbstractThe best explanatory approaches in the natural sciences are based on mathematical models. The COVID-19 pandemic or climate change illustrate the importance of mathematical modelling. This study focusses on the influence of external representations, texts, diagrams, and images, as well as mathematical expertise, on modelling competence for biochemical concepts. Especially the cross-curricular aspect of these tasks shows the influence of different science disciplines on concrete skills and abilities in biology. Consequently, the participants were asked to complete an enzyme kinetics task
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Viganò, Edoardo Luca, Erika Colombo, Davide Ballabio, and Alessandra Roncaglioni. "Artificial Intelligence Methods for Evaluating Mitochondrial Dysfunction: Exploring Various Chemical Notations Suitable for Neural Language Processing Models." In Lecture Notes in Computer Science. Springer Nature Switzerland, 2024. http://dx.doi.org/10.1007/978-3-031-72381-0_10.

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AbstractIn recent years, the integration of Artificial Intelligence and Machine Learning methods, such as Neural Language Processing (NLP), with biochemical and biomedical research has revolutionized the field of toxicology defining a profound advancement in our understanding of the toxicological effects of diverse chemical compounds on biological systems.Among various toxic effects, mitochondrial dysfunction has emerged as a crucial endpoint due to its role in various diseases related to the liver, heart brain, and more in general related to different physiological processes. Indeed, mitochon
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Rodrigo-Comino, Jesús, Artemi Cerdà, Stefan Doerr, et al. "Introduction: FIRElinks, a Community for Society and Science." In Fire Hazards: Socio-economic and Regional Issues. Springer International Publishing, 2024. http://dx.doi.org/10.1007/978-3-031-50446-4_1.

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AbstractFIRElinks (CA18135) originated from many efforts by a group of researchers after submitting a proposal for a COST Action. During four years, the main aim has been to develop an EU-spanning network of scientists and practitioners involved in forest fire research and land management with backgrounds such as fire dynamics, fire risk management, fire effects on vegetation, fauna, soil and water, and socioeconomic, historical, geographical, political perception, and land management approaches. Communities from different scientific and geographic backgrounds allowing the discussion of differ
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Conference papers on the topic "Biochemical research methods"

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Jhobalia, Chintan M., An Hu, Tingyue Gu, and Srdjan Nesic. "Biochemical Engineering Approaches to MIC." In CORROSION 2005. NACE International, 2005. https://doi.org/10.5006/c2005-05500.

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Abstract Microbiologically influenced corrosion (MIC) is a major problem in the oil and gas industry. A group of bacteria known as sulfate reducing bacteria (SRB) are most frequently the culprits. Although some new mitigation methods have started to emerge, such as adding nitrate to promote the growth of nitrate reducers that outcompete with SRB for nutrients, current mitigation methods still rely on biocides and biostats that either kill planktonic bacteria or inhibit their growth at low concentrations. A much higher concentration is needed to control established biofilms. Microorganisms are
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Madhuranthakam, Indu, and Martin Job. "Comparative Analysis of the Effects of Actual Versus Assumed Opioid Experience on the Regulation of Ventral Striatal Opioid Receptor Gene Expression." In 27th Annual Rowan-Virtua Research Day. Rowan University Libraries, 2023. https://doi.org/10.31986/issn.2689-0690_rdw.stratford_research_day.112_2023.

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Rationale: We conducted experiments to assess the effect of prior opioid experience on gene expression changes. We compared the current experimenter-imposed short versus extended-access conditions of opioid self-administration and developed a new quantitative method to determine their effectiveness in identifying the role of opioid experience in regulating opioid receptor expression levels in the ventral striatum (VS) using an oxycodone self-administration/abstinence model. Methods: In this study, male Sprague-Dawley rats (n=36) were trained for 20 days to self-administer oxycodone at 0.1 mg/k
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Paraschiv, Alina-Nicoleta, Milica Dima, Aurelia Diaconu, Elena Ciuciuc, and Mihaela Croitoru. "RESEARCH ON THE BEHAVIOR OF AN ASSORTMENT OF BELL PEPPER ON THE SANDY SOILS IN SOUTH-WEST OLTENIA ACCORDING TO THE CULTIVATION METHOD USED." In GEOLINKS Conference Proceedings. Saima Consult Ltd, 2021. http://dx.doi.org/10.32008/geolinks2021/b1/v3/40.

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The pedo-climatic conditions specific to sandy soils in southwest Oltenia determine differences in performing different phenophases of growth and development of plants, both between varieties and in the physiological behavior of the same variety under different yield conditions. Therefore, at the Dăbuleni Research and Development Station for Plant Culture on Sands, research was carried out on some physiological, biochemical and production processes at five varieties of bell peppers cultivated in the open fields and solar. Determinations of photosynthesis, foliar transpiration, the biochemical
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Kovalev, I., and N. Kovaleva. "BIOMARKERS AS INDICATORS OF PALEOENVIRONMENT." In Man and Nature: Priorities of Modern Research in the Area of Interaction of Nature and Society. LCC MAKS Press, 2021. http://dx.doi.org/10.29003/m2604.s-n_history_2021_44/189-197.

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Biomarkers, due to their specific structure and biochemical functions, under certain environmental conditions are resistant to degradation and mineralization in soils and therefore serve as molecular traces of paleobiota and terrestrial vegetation, as well as the intensity of biochemical processes occurring in the biosphere not only in the present, but also in the past. The group composition of humus, optical densities in the visible and ultraviolet parts of the spectrum, chlorophyll, lipids, fatty acid spectrum, lignin phenols in combination age of soils are recommended as promising methods f
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Erst, A. S., E. Yu Mitrenina, W. Wang, M. V. Skaptsov, and V. A. Kostikova. "How many species of Eranthis (Ranunculaceae) are there in Siberia and Far East part of Russia? An integrative taxonomic approach." In Problems of studying the vegetation cover of Siberia. TSU Press, 2020. http://dx.doi.org/10.17223/978-5-94621-927-3-2020-51.

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For Siberian and Far East Eranthis systematics and taxonomy problems solving the integrative approach which includes morphometrical, cytological, molecular-philogenetical and biochemical methods has been applied. Complex obtained data has showed the genus population heterogeneity within the territory under study. Based on integrative taxonomy the new species – Eranthis tanhoensis was described. Our further research will allow study hybrids and evolution of Siberian and Far East taxa.
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Lupascu, Galina, Svetlana Gavzer, Nicolae Cristea, Angela Rudacova, and Ala Cherdivara. "Studies of variability and inheritability of productivity and quality characters in common wheat." In Conferința științifică națională cu participare internațională "Integrare prin cercetare și inovare", dedicată Zilei Internaționale a Științei pentru Pace și Dezvoltare. Moldova State University, 2025. https://doi.org/10.59295/spd2024n.16.

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The article presents the results of the genetic-environmental control study of the productivity elements of the common autumn wheat spike (7 genotypes during the years 2020-2022). The objects of the work: i) recording the elements of productivity; ii) establishing the weight (%) of the year, genotype, year x genotype interaction factors of in the source of variability character; iii) calculation of genetic parameters of variability and heritability, heritability coefficient; iv) determination of the biochemical quality of wheat grains. Research methods: biometric, biochemical, mathematical, st
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Bulatova, M. V., S. V. Usova, E. S. Bashkina, N. S. Kutserubova, and M. P. Bogryantseva. "COMPARATIVE EVALUATION OF METHODS FOR THE CONTROL OF BACTERIAL ENDOTOXINS IN IMMUNOBIOLOGICAL MEDICINES." In XI МЕЖДУНАРОДНАЯ КОНФЕРЕНЦИЯ МОЛОДЫХ УЧЕНЫХ: БИОИНФОРМАТИКОВ, БИОТЕХНОЛОГОВ, БИОФИЗИКОВ, ВИРУСОЛОГОВ, МОЛЕКУЛЯРНЫХ БИОЛОГОВ И СПЕЦИАЛИСТОВ ФУНДАМЕНТАЛЬНОЙ МЕДИЦИНЫ. IPC NSU, 2024. https://doi.org/10.25205/978-5-4437-1691-6-53.

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Despite the fact that the list of pyrogens is quite extensive, in the field of quality control of injectable medicines, pyrogens produced by gram–negative bacteria — bacterial endotoxins are mainly of practical importance [1]. Their significant presence in ready-made injectable dosage forms and active pharmaceutical substances can have a significant effect on the human body, causing allergic and inflammatory reactions, tissue damage and can even lead to serious violations of biochemical processes. According to the requirements of the international and domestic standard, which contains a set of
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Dutta, Debashis, and Naoki Yanagisawa. "Microfluidic Devices for Enhancing the Sensitivity of ELISA Methods." In ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2011. http://dx.doi.org/10.1115/icnmm2011-58284.

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Enzyme-linked immunosorbent assays (ELISA) are critically important tools in biological research, allowing the presence and concentrations of a wide variety of key biochemical intermediates to be determined. While the signal amplification that is the core advantage of ELISA methods is impressive, it is nevertheless the case that it is insufficient for some particularly demanding challenges in terms of sensitivity, assay time, or sample size. In this paper, we discuss three different approaches developed in our laboratory that can improve the sensitivity of ELISA methods by 2–3 orders of magnit
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Abd, Huda, and Abdul Majeed Ahmed. "Determination of Trace Elements (Copper, Zinc and Iron) and some Biochemical Variables in Patients with Kidney Failure Using a Spectrophotometer." In 5th International Conference on Biomedical and Health Sciences. Cihan University-Erbil, 2024. http://dx.doi.org/10.24086/biohs2024/paper.1248.

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Abstract—Background: A disorder known as chronic Kidney Disease (CKD) causes a significant loss of many kidney function units, nephrons, which negatively affects kidney functions and leads to their decline. It also results in a decrease in kidney mass, which in turn leads to hypertrophy of the remaining nephrons. Aims: The study's objective was to ascertain the kidney failure patients' trace element and biochemical parameter concentrations. Methods and Material: The study included drawing blood samples from 40 healthy individuals who did not suffer from any chronic diseases, aged between 20-40
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Ibrahim, NoorAlhuda, Wasnaa Mohammad, Sanan Abdalwahab, and Zahid Mohammed. "Serum Level of osteopontin Associated with Heart Disease in Iraqi Male." In 5th International Conference on Biomedical and Health Sciences. Cihan University-Erbil, 2024. http://dx.doi.org/10.24086/biohs2024/paper.1363.

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Background: Heart disease is a serious health-care issue in males and females, with high morbidity, death, and costs .Men had a higher chance of developing Heart disease, whereas women were leaser than . Aim: Osteopontin (OPN) activity was measured by comparing heart disease patients and healthy control subjects in this research. It is critical to use biochemical markers as reliable predictors. Osteopontin biochemical factors in patients with heart disease was assessed and their relationships studied as a result. Methods: Blood samples were collected from 90 male persons, age range (40-62) yea
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Reports on the topic "Biochemical research methods"

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Jander, Georg, Gad Galili, and Yair Shachar-Hill. Genetic, Genomic and Biochemical Analysis of Arabidopsis Threonine Aldolase and Associated Molecular and Metabolic Networks. United States Department of Agriculture, 2010. http://dx.doi.org/10.32747/2010.7696546.bard.

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Since the amino acids threonine and isoleucine can be limiting in mammalian diet and there is interest in increasing their abundance in certain crop plants. To meet this need, a BARD proposal was written with two main research objectives: (i) investigate new avenues for manipulating threonine and isoleucine content in plants and (ii) study the role of threonine aldolase in plant metabolism. Research conducted to meet these goals included analysis of the sub-cellular localization of threonine aldolase in the plant, analysis of metabolic flux in developing embryos, over- and under-expression of
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Crisosto, Carlos, Susan Lurie, Haya Friedman, Ebenezer Ogundiwin, Cameron Peace, and George Manganaris. Biological Systems Approach to Developing Mealiness-free Peach and Nectarine Fruit. United States Department of Agriculture, 2007. http://dx.doi.org/10.32747/2007.7592650.bard.

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Peach and nectarine production worldwide is increasing; however consumption is flat or declining because of the inconsistent eating quality experienced by consumers. The main factor for this inconsistent quality is mealiness or woolliness, a form of chilling injury that develops following shipping periods in the global fruit market today. Our research groups have devised various postharvest methods to prolong storage life, including controlled atmosphere and delayed storage; however, these treatments only delay mealiness. Mealiness texture results from disruption of the normal ripening process
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Jordan, Ramon L., Abed Gera, Hei-Ti Hsu, Andre Franck, and Gad Loebenstein. Detection and Diagnosis of Virus Diseases of Pelargonium. United States Department of Agriculture, 1994. http://dx.doi.org/10.32747/1994.7568793.bard.

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Pelargonium (Geranium) is the number one pot plant in many areas of the United States and Europe. Israel and the U.S. send to Europe rooted cuttings, foundation stocks and finished plants to supply a certain share of the market. Geraniums are propagated mainly vegetatively from cuttings. Consequently, viral diseases have been and remain a major threat to the production and quality of the crop. Among the viruses isolated from naturally infected geraniums, 11 are not specific to Pelargonium and occur in other crops while 6 other viruses seem to be limited to geranium. However, several of these v
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Sessa, Guido, та Gregory Martin. MAP kinase cascades activated by SlMAPKKKε and their involvement in tomato resistance to bacterial pathogens. United States Department of Agriculture, 2012. http://dx.doi.org/10.32747/2012.7699834.bard.

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The research problem: Pseudomonas syringae pv. tomato (Pst) and Xanthomonas campestrispv. vesicatoria (Xcv) are the causal agents of tomato bacterial speck and spot diseases, respectively. These pathogens colonize the aerial parts of the plant and cause economically important losses to tomato yield worldwide. Control of speck and spot diseases by cultural practices or chemicals is not effective and genetic sources of resistance are very limited. In previous research supported by BARD, by gene expression profiling we identified signaling components involved in resistance to Xcvstrains. Follow u
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McClure, Michael A., Yitzhak Spiegel, David M. Bird, R. Salomon, and R. H. C. Curtis. Functional Analysis of Root-Knot Nematode Surface Coat Proteins to Develop Rational Targets for Plantibodies. United States Department of Agriculture, 2001. http://dx.doi.org/10.32747/2001.7575284.bard.

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The goal of this research was to provide a better understanding of the interface between root-knot nematodes, Meloidogyne spp., and their host in order to develop rational targets for plantibodies and other novel methods of nematode control directed against the nematode surface coat (SC). Specific objectives were: 1. To produce additional monoclonal SC antibodies for use in Objectives 2, 3, and 4 and as candidates for development of plantibodies. 2. To determine the production and distribution of SC proteins during the infection process. 3. To use biochemical and immunological methods to pertu
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Hodges, Thomas K., and David Gidoni. Regulated Expression of Yeast FLP Recombinase in Plant Cells. United States Department of Agriculture, 2000. http://dx.doi.org/10.32747/2000.7574341.bard.

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Research activities in both our laboratories were directed toward development of control of the FLP/frt recombination system for plants. As described in the text of the research proposal, the US lab has been engaged in developing regulatory strategies such as tissue-specific promoters and the steroid-inducible activation of the FLP enzyme while the main research activities in Israel have been directed toward the development and testing of a copper-regulated expression of flp recombinase in tobacco (this is an example of a promoter activation by metal ions). The Israeli lab hat additionally com
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Uni, Zehava, and Peter Ferket. Enhancement of development of broilers and poults by in ovo feeding. United States Department of Agriculture, 2006. http://dx.doi.org/10.32747/2006.7695878.bard.

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The specific objectives of this research were the study of the physical and nutritional properties of the In Ovo Feeding (IOF) solution (i.e. theosmostic properties and the carbohydrate: protein ratio composition). Then, using the optimal solution for determining its effect on hatchability, early nutritional status and intestinal development of broilers and turkey during the last quarter of incubation through to 7 days post-hatch (i.e. pre-post hatch period) by using molecular, biochemical and histological tools. The objective for the last research phase was the determination of the effect of
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