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Dissertations / Theses on the topic 'Biochemistry and Cell Biology'

Author: Grafiati

Published: 4 June 2021

Last updated: 20 February 2023

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1

Camacho, Diogo Mayo. "In silico cell biology and biochemistry: a systems biology approach." Diss., Virginia Tech, 2007. http://hdl.handle.net/10919/27960.

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In the post-"omic" era the analysis of high-throughput data is regarded as one of the major challenges faced by researchers. One focus of this data analysis is uncovering biological network topologies and dynamics. It is believed that this kind of research will allow the development of new mathematical models of biological systems as well as aid in the improvement of already existing ones. The work that is presented in this dissertation addresses the problem of the analysis of highly complex data sets with the aim of developing a methodology that will enable the reconstruction of a biological

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2

Agüera-González, Sonia. "Cell biology on NKG2D ligands and NK cell recognition." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609348.

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3

Chambers, Jeremy W. "Studies in the biochemistry and cell biology of Trypanosoma brucei hexokinases." Connect to this title online, 2007. http://etd.lib.clemson.edu/documents/1202500780/.

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4

Trotman, Jackson B. "New Insights into the Biochemistry and Cell Biology of RNA Recapping." The Ohio State University, 2018. http://rave.ohiolink.edu/etdc/view?acc_num=osu1523896565730483.

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5

Delorme, Marilyne. "Downregulation of ATRX disrupts cell proliferation and cell cycle progression." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/27627.

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ATRX is a chromatin remodelling protein of the SNF2 family of chromatin remodelling proteins. Mutations in the ATRX gene have been shown to cause the ATR-X syndrome, an X-linked mental retardation disorder. ATRX is part of a chromatin-remodelling complex with Daxx that localizes to PML nuclear bodies or pericentromeric heterochromatin and is thought to regulate gene expression. In mice, Atrx inactivation results in embryonic lethality whereas conditional forebrain specific Atrx ablation showed impaired development and disorganization of the cortex. Furthermore, ATRX phosphorylation was shown t

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6

Hussey, P. J. "Studies on the molecular and cell biology of plant tubulin." Thesis, University of Kent, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376791.

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7

Ye, Qing. "LIPASE-KINASE ASSOCIATIONS INVOLVING PLD2, JAK3 AND FES THAT UNDERLIE CANCER CELL PROLIFERATION AND INVASION." Wright State University / OhioLINK, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=wright1421939242.

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8

Chu, Wei. "Mouse Mast Cell Proteases: Induction, Molecular Cloning, and Characterization." Digital Commons @ East Tennessee State University, 1991. https://dc.etsu.edu/etd/2656.

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Tryptase, a mast cell-specific serine protease with trypsin-like specificity, has been identified in a mouse mast cell line (ABFTL-6) based on it's enzymatic activity, inhibition properties, and cross-reactivity to a human mast cell tryptase antibody. The effects of fibroblast-conditioned medium and sodium butyrate on ABFTL-6 mast cell differentiation and tryptase expression have been examined. ABFTL-6 mouse mast cells undergo phenotypic changes upon culturing in media supplemented with fibroblast-conditioned media at 50% or 1 mM sodium butyrate. The induced cells increased in

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9

Petosa, Adamo. "Isolation of human scFv expressing cells from a yeast library using magnetic and fluorescence activated cell sorting." Thesis, McGill University, 2006. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=101733.

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The effective and efficient generation of both antibodies and antibody fragments to proteins of interest is vital, as antibodies and antibody fragments are required for an ever-increasing variety of therapeutic, diagnostic and analytical applications. The single chain variable fragment (scFv) is an antibody fragment consisting of a heavy chain variable region (VH) and a light chain variable region (VL) joined together by a flexible polypeptide linker. In 2003, Feldhaus et al. developed a nonimmune human scFv surface display library, in Saccharomyces cerevisiae , containing 109 different scFvs.

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10

Bainor, Anthony J. "Elucidating the Role of SIN3B as a Regulator of Cell Cycle Exit." Thesis, New York University, 2017. http://pqdtopen.proquest.com/#viewpdf?dispub=10604607.

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<p> Progression through the mammalian cell cycle is a tightly regulated process that allows cells to replicate their genomes and divide properly. In growth factor-deprived conditions or in response to stress, the cell will exit the cell cycle either reversibly through quiescence, or permanently via senescence. Studies have shown that the SIN3 family of proteins plays a crucial role in these cell cycle exit processes. SIN3 proteins are highly conserved, and exist in mammals as two family members: SIN3A and SIN3B, which function as flexible scaffolding proteins to assemble co-repressor complexes

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11

Upcher, William R. "Biochemical studies of the cohesin complex." Thesis, University of Oxford, 2012. http://ora.ox.ac.uk/objects/uuid:e9a77a9c-40a5-466d-a894-c7fefeddef52.

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The accurate inheritance of genetic material depends upon the establishment and maintenance of sister chromatid cohesion. Replicated chromosomes are topologically encircled by the large, tripartite protein complex cohesin, allowing bi-orientation in mitosis. To entrap and reversibly dissociate from DNA, the annular complex structure must be disrupted at either the hinge domain between Smc1 and Smc3, or the interfaces created by the kleisin subunit Scc1 bridging the two ABC-like ATPase domains. The aim of this work was to characterise the cohesin complex loading and releasing mechanisms by exam

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12

Fernández, Lola. "Studies on the biochemistry and cell biology of the glycosyl-phosphatidylinositol (GPI)-anchored NKG2D-ligands." Thesis, University of Cambridge, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.609534.

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13

Sipe, Conor W. "Cloning and Functional Characterization of Hypoxia-Inducible Factor 1alpha Upstream Regions in Xenopus laevis." W&M ScholarWorks, 2003. https://scholarworks.wm.edu/etd/1539626407.

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14

Reimer, Michael. "Characterization of IQGAP1 Protein in Areas of Cell Retraction." Thesis, Southern Illinois University at Edwardsville, 2015. http://pqdtopen.proquest.com/#viewpdf?dispub=1582876.

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<p> IQGAP1 interacts with numerous binding partners through a calponin homology domain (CHD), a WW motif, IQ repeats, a Ras GAP related domain (GRD), and a conserved C-terminal (CT) domain. Among various biological and cellular functions, IQGAP1 plays a role in cell-matrix interactions and actin cytoskeleton dynamics in membrane ruffling and lamellipodium protrusion. Phosphorylation in the CT domain regulates intramolecular interaction and IQGAP1 cellular activity. In a recent study, we discovered that IQGAP1 surprisingly localizes to actively retracting edges, instead of protruding areas, in

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15

Poole, Charla N. "Cardiovascular therapeutics derived from the paracrine biology of adult human progenitor cells." Thesis, Tulane University, 2014. http://pqdtopen.proquest.com/#viewpdf?dispub=3628932.

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<p> Adult multipotent stromal cells (MSCs) may repair tissue through the action of secreted factors on endogenous stem/progenitor cells. We determined the effects of MSC-secreted factors on adult cardiac progenitor cells (CPCs). Serum-free conditioned medium (CdM) was collected from MSCs isolated by plastic adherence (MSCs) and by magnetic sorting against the p75 nerve-growth factor receptor (p75MSCs). Compared to serum-free medium (α-MEM), CdM significantly increased adult rat CPC proliferation in a concentration-dependent manner, led to phosphorylation (Tyr<sup>705</sup>) and nuclea

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16

Ng, Shyh Chang. "Regulation of Stem Cell Metabolism by the Lin28/let-7 Axis." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:11217.

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My PhD thesis is focused on two fundamental aspects of stem cell metabolism: (1) the role of Lin28 in programming stem cell metabolism, and (2) how metabolism in turn fuels and governs pluripotency. Our studies led us to discover that the stem cell factor Lin28a promotes gigantism by enhancing glucose metabolism in mice, coinciding with discoveries that LIN28B polymorphisms influence height variation in human GWAS. Subsequently, we discovered that the Lin28/let-7 pathway controls glucose metabolism by orchestrating the upregulation of multiple insulin-PI3K-mTOR components, particularly in skel

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17

Sakanovic, Alenko. "EphrinB2 reverse signaling in endothelial cell migration and actin remodeling." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/27030.

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Angiogenesis, the formation of new blood vessels from preexisting vasculature, is exaggerated in cancer. A ligand that may be involved in angiogenesis is ephrinB2, which upon binding to its cognate receptor on an adjacent cell, becomes phosphorylated on tyrosine residues and transduces an as yet uncharacterized signal inside the cell. To examine reverse signaling pathways mediated by ephrinB2, ephrinB2 mutants were generated in which five tyrosine residues in the cytoplasmic tail were mutated to phenylalanine by site directed mutagenesis to effectively block the putative signal transduction pa

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18

Ibarra-Sánchez, Maria. "Biochemical and cellular function of t-cell protein tyrosine phosphatase." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82895.

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Tyrosine phosphorylation is one of the most important post-translational modifications used by cells to respond to external stimuli. Two different sets of enzymes control the tyrosine phosphorylation levels of proteins: the protein tyrosine kinases and the protein tyrosine phosphatases. The identification of the cellular function of these enzymes is a key step in the understanding of many biological processes, such as proliferation, differentiation and apoptosis. We used a mouse model deficient in the protein tyrosine phosphatase TC-PTP to identify its biological and biochemical functio

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19

Buchanan, Fritz G. "Endogenous Alkylglycerol Functions As a Mediator of Protein Kinase C Activity and Cell Proliferation." Digital Commons @ East Tennessee State University, 1997. https://dc.etsu.edu/etd/2885.

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To explore the possibility that 1-O-alkyl-sn-glycerol (alkylglycerol) may serve a regulatory role in the control of cell proliferation or PKC activity, we examined the ability of alkylglycerol to influence PKC activity and subcellular distribution as well as the ability of alkylglycerol to effect cell proliferation. MDCK cells grown to confluence show a loss of PKC activity associated with the membrane, as reported in fibroblasts. Preconfluent cultures of MDCK cells have a high level of PKC activity associated with the membrane. However, treatment of preconfluent cultures with

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20

Arojo, Omotooke Ajoke. "The Role of Sin 1-mTORC2 in the Regulation of T Cell Homeostasis." Thesis, Yale University, 2018. http://pqdtopen.proquest.com/#viewpdf?dispub=10783434.

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<p> In healthy individuals, T cells generally exist in a state of equilibrium or homeostasis. This homeostasis is achieved by a tight and dynamic regulation of signaling cascades that control T cell numbers, localization, metabolism, and differentiation at any given time. Dysregulation of T cell homeostasis underlies several immunopathologic conditions including autoimmune diseases and cancer, hence the need to better understand molecular mechanisms that control T cell homeostasis. The mechanistic target of rapamycin (mTOR) is a critical hub within several important signaling cascades that are

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21

Miller, Christina Roshek 1969. "Photosensitive liposome-cell interaction in vitro." Diss., The University of Arizona, 1998. http://hdl.handle.net/10150/288913.

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Bennett and O'Brien [Biochemistry 1995 34, 3102] showed that the ultraviolet light exposure of two-component large unilamellar liposomes (LUV) composed of a 3:1 molar mixture of dioleoylphosphatidylethanolamine (DOPE) and 1,2-bis[10-(2'-hexadienoyloxy)-decanoyl]-sn-glycero-3-phosphatidylcholine (bis-SorbPC) facilitated liposome fusion. The rate and extent of fusion was dependent on the extent of photopolymerization, the temperature, and the pH. Here, the effect of the molar lipid ratio of DOPE/bis-SorbPC liposomes on the temperature for the onset of fusion, was characterized by changing the r

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22

Rodrigues, Sonia Patricia. "The function of the Crk adapter proteins in cell migration and invasion /." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=80866.

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Epithelial morphogenesis is an important signaling program in normal embryonic development involving cell proliferation, migration, invasion and cell matrix turnover and also during tumourigenesis. The Crk adaptor family of proteins has been implicated as promoters and mediators of such biological responses downstream of various extracellular signals. Our laboratory has defined the involvement of Crk adaptor proteins in the signaling of Met-dependent anchorage-independent growth, cell dispersal, cell invasion and epithelial morphogenesis. In an MDCK epithelial cell model CrkII overexpre

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23

Li, Ge. "Cell physiology, biochemistry, and molecular biology of 5-aminolevulinic acid-induced protoporphyrin IX in normal, immortalized, transfected, and malignant cells." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/tape17/PQDD_0005/NQ27837.pdf.

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24

Niedowicz, Dana M. "The mechanisms of hyperglycemia-induced oxidative stress in human erythrocytes." [Bloomington, Ind.] : Indiana University, 2006. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pqdiss&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&rft_dat=xri:pqdiss:3204307.

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Thesis (Ph. D.)--Indiana University, Dept. of Biology, 2006.<br>Source: Dissertation Abstracts International, Volume: 67-01, Section: B, page: 0043. Adviser: David L. Daleke. "Title from dissertation home page (viewed Feb. 21, 2007)."

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25

Abrol, Meena. "The role of Sir2alpha in myogenesis." Thesis, University of Ottawa (Canada), 2003. http://hdl.handle.net/10393/26434.

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Protein acetylation is becoming recognized as an important modification in the regulation of many cellular pathways. The silent information regulator 2 (Sir2) proteins are a family of NAD-dependent protein deacetylases. Sir2 protein function in yeast has extensively been characterized as an essential mediator of gene silencing that occurs at the mating type loci, the telomeres and the rDNA regions. There are Sir2 homologues from yeast to mammals, but little is known about the role of Sir2 in the mammalian system. There is evidence that myogenesis is regulated by the acetylation state of the hi

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26

Gangaraju, Sandhya. "Role of mitofusin2 in the regulation of mitochondrial dynamics." Thesis, University of Ottawa (Canada), 2003. http://hdl.handle.net/10393/26483.

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Mitochondria in all cell types undergo frequent fission and fusion events, and these dynamics determine the overall morphology of the organelle in cells. Two important GTPases have been recently identified that regulate mitochondrial membrane activity, a dynamin related protein (DRP1) required for fission, and the novel fusion GTPase, Mitofusin2. Mitofusin2 is an outer mitochondrial membrane protein and, like other GTPases involved in membrane fusion events, the N-terminal GTPase domain is exposed to the cytosol, such that it could interact with and recruit potential cytosolic proteins. The wo

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27

Taylor, Rebecca Ann C. "VSV infection of resting and activated T lymphocytes." Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/26782.

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Resting T lymphocytes are uniquely resistant to VSV even at high multiplicities of infection but they can be rendered fully permissive for VSV replication following in-vitro activation with monoclonal anti-CD3 and PMA with ionomycin. The block to VSV replication is at the level of viral RNA production and is independent of transcription following infection. T lymphocytes must be activated before infection for at least 24 hours to be rendered susceptible to VSV and transcription is an absolute requirement during this process. Fusion of resting and activated T cells results in a resistant cell i

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28

Dunkley, Rosamund. "Ubiquitous over-expression of human X-linked inhibitor apoptosis (XIAP) in a transgenic mouse and implications for tumorigenesis." Thesis, University of Ottawa (Canada), 2005. http://hdl.handle.net/10393/26895.

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The suppression of programmed cell death is an essential alteration in the transformation from normal to neoplastic growth. The X-linked inhibitor of apoptosis (XIAP) is a potent suppressor of apoptosis. It is a member of the Inhibitor of Apoptosis (IAP) family and functions by inhibiting caspases 3, 7 and 9 critical proteases in the process of programmed cell death. XIAP expression levels are frequently elevated in cancer cell lines and tumors, yet the link between XIAP expression and tumorigenesis has not been demonstrated experimentally. The objective of this project is to determine whether

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29

Czechura, Pawel. "Saturated neoglycopolymers for tissue engineering." Thesis, University of Ottawa (Canada), 2006. http://hdl.handle.net/10393/27121.

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Norbornene monomers bearing carbohydrate groups of relevance for tissue engineering were synthesized via the norbornene acid chlorides, transformed into their ROMP polymers, and reduced to yield saturated neoglycopolymers. These materials bear either O-glycoside groups designed to cross-link collagen via reaction of the ring-open sugar with free NH2 groups of lysine, or C-glycoside groups. The latter are intended for use as the central block in triblock copolymers terminated with blocks capable of crosslinking: they serve only as potentially biocompatible "spacers" to help span the interlamell

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30

Dykstra, Natalie. "The investigation of microbial-intestinal epithelial cell interactions in the gut and their effects on inhibitor of apoptotic proteins (IAPs)." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/27978.

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Probiotic bacteria such as Lactobacillus plantarum strain 299v (Lp299v) have been shown to upregulate facets of innate immunity. This study assessed whether Lp299v prevents apoptosis in intestinal epithelial cells (IECs) when faced with cytokine challenge. Both in vitro and in vivo systems were exposed for a pre-determined time to Lp299v or strain Lpadh---a non-adherent derivative of Lp299v. HT-29 cells were then challenged with cytokine mixture (TNF-alpha, IFN-gamma, and IL-1a) to imitate conditions of inflammation. To assess for apoptosis, we evaluated both TUNEL analysis and caspase activit

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31

Ewing, Robyn. "Analysis of the subcellular trafficking of the glucocorticoid receptor and properties of the ligand binding domain." Thesis, University of Ottawa (Canada), 2008. http://hdl.handle.net/10393/27979.

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The glucocorticoid receptor (GR) is a ligand dependent transcription factor and member of the nuclear receptor superfamily. Nuclear import and export of transcription factors is accomplished through nuclear localization signals (NLS) and nuclear export signals (NES), respectively. We have determined that L687 and L690 of rat GR are necessary for the characteristically slow nuclear export of GR and may be included in the signal sequence responsible for directing post-agonist withdrawn GR from the nucleus to the cytoplasm. We also suggest that L687 and L689 of rat GR are required for efficient N

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32

Guzzo, Rosa M. "Sarcolemmal membrane associated proteins: Structure-function analyses and localization studies." Thesis, University of Ottawa (Canada), 2003. http://hdl.handle.net/10393/29048.

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Previous work from our lab identified a novel cDNA encoding a family of tail-anchored coiled-coil integral membrane proteins termed SLMAPs (sarcolemmal membrane associated proteins) (Wigle et al., 1997). Subsequent studies determined that SLMAPs are encoded by a single gene, and alternative splicing yields three SLMAP isoforms, including two muscle-specific variants (SLMAP1, SLMAP2) and a ubiquitously expressed isoform (SLMAP3). Here, I report a series of studies designed to examine putative novel and isoform specific functions of SLMAPs in striated muscle and fibroblast cells. The tissue dist

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33

Bou, Khalil Maroun. "Biophysical and biochemical properties of the mammalian male germ cell specific sulfogalactosylglycerolipid (SGG): Contribution to the structure and zona pellucida affinity of pig sperm raft membranes." Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/29079.

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Raft membranes are implicated in cell adhesion. Here, we demonstrated that rafts isolated from hypermotile capacitated pig sperm as low-density Triton X-100 insoluble membranes had the ability to bind specifically to homologous zonae pellucidae (ZP). This binding was dependent on pig ZP3alpha sulfoglycoprotein, a major player in intact sperm binding. The male germ cell specific sulfogalactosylglycerolipid (SGG) was a sperm raft component and participated in sperm raft-ZP binding, since rafts pretreated with anti-SGG IgG/Fab had decreased ability to bind to the ZP dose dependently. SGG may also

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34

Stewart, Nicolas Andre Stirling. "In vacuo chemical modifications of proteins and peptides." Thesis, University of Ottawa (Canada), 2004. http://hdl.handle.net/10393/29170.

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A novel approach for the chemical modification of proteins and peptides was developed, namely the chemical modification under vacuum (in vacuo) of lyophilized proteins and peptides. This method was used with iodomethane to produce peptides with a permanent positive charge by converting their amino groups to trimethylammonium derivatives for analysis by matrix assisted laser desorption ionization mass spectrometry (MALDI MS). Peptides with such a permanent charge have a higher ionization yield under MALDI MS resulting in a dramatic increase in detection. The signal intensity of the trimethylamm

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35

Korley, Robert E. C. "Characterization of a major protein of the mouse perinuclear theca." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1999. http://www.collectionscanada.ca/obj/s4/f2/dsk1/tape2/PQDD_0035/MQ64383.pdf.

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36

Harrouk, Wafa. "Mitogen-activated protein kinase during oocyte growth in the mouse." Thesis, McGill University, 1994. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=55499.

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In this study, the role of MAP kinase in the acquisition of meiotic competence in growing oocytes was investigated. The results presented in this thesis show that two species of MAP kinase, p42 and p44, are present in their unphosphorylated forms in oocytes as early as 5 days of age. At this age, oocytes are small and have not acquired the capacity to resume meiosis. They are referred to as meiotically incompetent. MAP kinase continues to be present throughout the growth phase and up to the acquisition of meiotic competence.<br>In growing mouse oocytes, a group of partially competent oocytes a

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37

Schroeder, Hans R. "S-acylation and intracellular targeting of lipid-modified proteins and model lipopeptides." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36812.

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Three related studies were performed to better characterize the intracellular process of protein S-acylation in mammalian cells. The first study focused on the use of cysteinyl-containing fluorescent lipopeptides which mimic the N-terminal of various S-acylable intracellular regulatory proteins. These lipopeptides diffuse rapidly between membranes and are efficiently S-acylated by a variety of mammalian cells. S-acylation appears to be enzymatic by various criteria and is highly selective for cysteinyl as opposed to serinyl residues. Fluorescence microscopy revealed that the plasma membrane is

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Lee, Menq-Jer. "Biochemical characterization of statin, a protein marker specific fofor nonproliferating cells, and identification of its associated proteins in cultured human diploid fibroblasts." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41296.

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Statin, a 57 kilodalton (kDa) protein expressed specifically in nuclei of non-proliferative cells (Wang, 1985a, c), is a phosphoprotein, phosphorylated at serine residues. By comparing immunoprecipitation and immunoblotting analyses, I find that p57$ sp{ rm statin}$ in vivo associates with other cellular proteins with molecular masses of 45 kDa (p45) and 110 kDa (p110).<br>The p45 statin-associated protein is a serine/threonine kinase. The specificity of the association of statin and p45 kinase is strongly supported by the fact that the kinase activity of p45 correlates both qualitatively and

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Brignole, Claudine. "The adenovirus E4orf4 protein induces G2 / M arrest and cell death by inhibiting PP2A phosphatase activity regulated by the B Alpha subunit /." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81604.

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The human adenovirus E4orf4 protein is toxic in human tumor cells and yeast. In both cases, interaction of E4orf4 with the Balpha/Cdc55 regulatory subunit of protein phosphatase 2A (PP2A) appears to be critical for cell killing; however, the effect of E4orf4 binding on PP2A is not known. Balpha is one of several B subunits that form holoenzymes with A and C subunits and regulate PP2A substrate specificity. In the present studies the effects of E4orf4 on PP2A activity was examined, as well as the role of PP2A in E4orf4-mediated cell death. Previously it was shown that binding of E4orf4 h

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Roudaia, Liya. "Characterization of RasGAP-SH3-binding protein's functions." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=82418.

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RasGAP-SH3 binding protein (G3BP) is a player in the ras signal transduction pathway and is involved in mRNA turnover. G3BP is a multifunctional protein affecting cell growth, cell stress responses, and cell transformation. The goal of this study was to define ex vivo function(s) of G3BP in several systems. We developed two RNA interference methods to knockdown G3BP in murine cell lines. We observed that the partial plasmid-mediated RNAi knockdown of MmuG3BP1 does not affect muscle cell differentiation. To improve efficiency, we successfully developed lentiviral-mediated RNAi against Mm

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Cardillo, Marricco Nadia. "Characterization of casein kinase I-c2, as a serinethreonine protein kinase associated with the adaptor protein, Nck." Thesis, McGill University, 1998. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=21603.

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CKI-gamma2 is a serine/threonine protein kinase of unknown biological function. This study is the first to detect CKI-gamma2, as mRNA and protein, widely expressed in mouse tissues and mammalian cells. In addition, CKI-gamma2 as a protein of 75kDa was immunologically detected coprecipitating with Nck, an adaptor protein involved in receptor tyrosine kinase (RTK) signaling. CKI-gamma2 coprecipitated with Nck presents similar enzymatic properties as the recombinant and endogenous CKI-gamma2, demonstrating that the immunoreactive p75 CKI-gamma2 associated with Nck is indeed CKI-gamma2. These resu

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42

Park, Minwoo. "2',3'-Cyclic nucleotide 3'-phosphodiesterase : investigation of interaction with Fyn tyrosine kinase during the development of nervous system, and mitochondrial import of CNP2 isoform." Thesis, McGill University, 2004. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81423.

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2',3'-Cyclic nucleotide 3'-phosphodiesterase is a protein highly expressed in oligodendrocytes in the central nervous system, and it is believed to be an important regulator of myelination. In this report, two aspects of CNP are investigated, each introduced in their own chapters.<br>First, a possible interaction of CNP with Fyn tyrosine kinase during myelination is investigated. Fyn is an important factor known to be active during the process of myelination, and CNP contains a number of possible tyrosine phosphorylation sites. Furthermore, their presence in an isolated domain of cell m

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Minuk, Jeffrey. "Studies on the intracellular sorting of the two myelin-associated glycoprotein isoforms." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=23414.

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Myelin-associated glycoprotein (MAG) is amongst the earliest expressed myelin specific proteins. Its precise function remains unknown, though evidence supports a role for cell-cell adhesion. MAG may also act as a spacer molecule and could be involved in signal transduction. MAG is expressed as two isoforms, designated as L-MAG and S-MAG. Both share identical extracellular and transmembrane domains but differ in their cytoplasmic domains. L-MAG is expressed earlier during myelination than S-MAG. These features, as well as others, suggest that the isoforms have different functions. To confirm th

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Côté, Richard Gaston. "Characterization of the retinoblastoma binding protein 2 (RBP2)." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=33388.

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The retinoblastoma (RB) family of proteins plays a pivotal role in cell cycle regulation. Its members, p105/Rb, p107 and p130, interact with the E2F and DP family of transcription factors to regulate transcription of essential cell cycle and DNA synthesis genes. Several reports have mapped the regulation of E217 by RB family members to the "pocket" domain of these proteins. We demonstrate here that RBP2, a pocket-binding protein that encodes multiple DNA-binding and protein-protein interaction domains, is a transcriptional repressor. Overexpression of RBP2 inhibits E2F-dependent transcription

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Tremblay, Gilles B. "Methylenetetrahydrofolate dehydrogenases in eucaryotic cells." Thesis, McGill University, 1995. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=40012.

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Methylenetetrahydrofolate dehydrogenases are expressed in mammalian cells and are required for the interconversian of one-carbon substituted folates. The trifunctional NADP-dependent dehydrogenase-cyclohydrolase-synthetase is localized in the cytoplasm whereas the bifunctional NAD-dependent dehydrogenase-cyclohydrolase is expressed in mitochondria suggesting they play different metabolic roles. Insect tissues also express both proteins but when immortalized into cell lines, the trifunctional enzyme is not detectable and the bifunctional enzyme is localized predominantly in the cytoplasm. The c

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Ajdukovic, Boris. "Dystrophin expression during skeletal myogenesis." Thesis, McGill University, 1992. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=56988.

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The factors that regulate dystrophin accumulation, and its association with other proteins in culture, are largely unknown. In this study I have examined the effects of chemical agents on dystrophin accumulation in culture, as well as determined whether dystrophin in culture has associations with glycoproteins in a similar fashion to that found in normal muscle tissue. My results show that the onset of detectable dystrophin accumulation occurs shortly after myoblast fusion has taken place, and increases rapidly thereafter as a percentage of total cell protein. The effects of depolarizing conce

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Taheri, Maryam. "Characterization of the structural determinants of two functions of human carcinoembryonic antigen (CEA) : intercellular adhesion and differentiation inhibition." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=36841.

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Human carcinoembryonic antigen (CEA) is a heavily glycosylated cell surface protein that belongs to the immunoglobulin superfamily (IgSF). It is overexpressed in a wide variety of human cancers. CEA functions in vitro, at least, as an intercellular adhesion molecule. It has also been shown that ectopic expression of CEA can block myogenic differentiation of rat L6 and mouse C2C12 myoblasts. Both the intercellular adhesion function of CEA as well as the inhibition of myogenic differentiation are dependent on homophilic binding of its extracellular domains. Previous studies have demonstrated tha

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Oliva, Rossella Norma. "Characterization of the isoform gamma 2 ([gamma] 2) of the serinethreonine protein kinase Casein Kinase I (CKI-[gamma] 2)." Thesis, McGill University, 2003. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=81369.

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Casein Kinase I (CKI) is one of the first serine/threonine protein kinase activities to be isolated and characterized. In vertebrates, CKI represent a multigene family with seven isoforms, alpha, beta, delta, ε, gamma1-3 for which, very little is known with respect to their biological functions. This study reports that stable overexpression of CKI-gamma2 into fibroblasts impairs cell morphology and the organization of the actin cytoskeleton in a kinase-dependent mechanism and cell proliferation through a kinase-independent mechanism. In addition, by immunofluorescence we determined the

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Terrone, Donato Gerardo. "Ras protein targeting in mammalian cells." Thesis, McGill University, 2005. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=98506.

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The Ras proteins (H-Ras, N-Ras and K-Ras4B) are monomeric GTP-binding proteins that play key roles in cell regulation. It has been proposed that correct plasma membrane localization of the Ras proteins requires 'two signals', the processed farnesylation sequence and an adjacent palmitoylation site(s) or polybasic sequence. First, to investigate potential proteinaceous binding partners for the K-Ras4B targeting sequence at the plasma membrane, an in vivo crosslinking analysis was carried out. Consistent with current suggestions that K-Ras4B interacts via electrostatic interactions with plasma m

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Li, Jing 1957. "Studies on iron-dependent regulation of transferrin receptor gene expression in chicken HD3 cells." Thesis, McGill University, 1996. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=27368.

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Expression of the transferrin receptor (TfR) gene is developmentally regulated in mammalian cells (1), an iron-dependent post-transcription regulatory mechanism has been demonstrated in non-erythroid cell (2,3). In order to understand the molecular mechanism underlying the iron-dependent regulation of the TfR gene expression in the differentiating erythroid cell, a virus-transformed chicken erythroblast cell line, HD3 was employed in this study. We have investigated the possible effects of iron on the TfR gene expression that might involve regulatory mechanisms at the transcriptional and/or th

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