Relevant bibliographies by topics / Biochemistry Laboratory

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Academic literature on the topic 'Biochemistry Laboratory'

Author: Grafiati
Published: 4 June 2021
Last updated: 6 February 2022

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Journal articles on the topic "Biochemistry Laboratory"

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Jovičić, Snežana, and Nada Majkić-Singh. "Medical Biochemistry as Subdiscipline of Laboratory Medicine in Serbia." Journal of Medical Biochemistry 36, no. 2 (April 1, 2017): 177–86. http://dx.doi.org/10.1515/jomb-2017-0010.

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SummaryMedical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia, and medical biochemist is the official name for the clinical chemist (or clinical biochemist). This is the largest sub-discipline of the laboratory medicine in Serbia. It includes all aspects of clinical chemistry, and also laboratory hematology with coagulation, immunology, etc. Medical biochemistry laboratories in Serbia and medical biochemists as a profession are part of Health Care System and their activities are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists (Clinical Chemists) in Serbia dates from 1945, when the Department of Medical Biochemistry was established at the Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate study program was established, educating Medical Biochemists under a special program. Since the academic year 2006/2007 the new five year undergraduate (according to Bologna Declaration) and four-year postgraduate program according to EC4 European Syllabus for Postgraduate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredited these programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Study of Medical Biochemistry), successful completion of the professional exam at the Ministry of Health after completion of one additional year of obligatory practical training in the medical biochemistry laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by the Serbian Chamber of Medical Biochemists. In order to present laboratory medical biochemistry practice in Serbia this paper will be focused on the following: Serbian national legislation, healthcare services organization, sub-disciplines of laboratory medicine and medical biochemistry as the most significant, education in medical biochemistry, conditions for professional practice in medical biochemistry, continuous quality improvement, and accreditation. Serbian healthcare is based on fundamental principles of universal health coverage and solidarity between all citizens.
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Majkić-Singh, Nada. "Education and Recognition of Professional Qualifications in the Field of Medical Biochemistry in Serbia." Journal of Medical Biochemistry 30, no. 4 (October 1, 2011): 279–86. http://dx.doi.org/10.2478/v10011-011-0013-7.

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Education and Recognition of Professional Qualifications in the Field of Medical Biochemistry in Serbia Medical biochemistry is the usual name for clinical biochemistry or clinical chemistry in Serbia, and medical biochemist is the official name for the clinical chemist (or clinical biochemist). This is the largest sub-discipline of the laboratory medicine in Serbia. It includes all aspects of clinical chemistry, and also laboratory hematology with coagulation, immunology, etc. Medical biochemistry laboratories in Serbia and medical biochemists as a profession are part of Health Care System and their activities are regulated through: the Health Care Law and rules issued by the Chamber of Medical Biochemists of Serbia. The first continuous and organized education for Medical Biochemists (Clinical Chemists) in Serbia dates from 1945, when the Department of Medical Biochemistry was established at the Pharmaceutical Faculty in Belgrade. In 1987 at the same Faculty a five years undergraduate branch was established, educating Medical Biochemists under a special program. Since school-year 2006/2007 the new five year undergraduate (according to Bologna Declaration) and postgraduate program of four-year specialization according to EC4 European Syllabus for Post-Gradate Training in Clinical Chemistry and Laboratory Medicine has been established. The Ministry of Education and Ministry of Public Health accredits the programs. There are four requirements for practicing medical biochemistry in the Health Care System: University Diploma of the Faculty of Pharmacy (Study of Medical Biochemistry), successful completion of the profession exam at the Ministry of Health after completion of one additional year of obligatory practical training in the medical biochemistry laboratories, membership in the Serbian Chamber of Medical Biochemists and licence for skilled work issued by the Serbian Chamber of Medical Biochemists. The process of recognition of a foreign higher education document for field of medical biochemistry is initiated on request by Candidate. The process of recognition of foreign higher education documents is performed by the University. In the process of recognition in Serbia national legislations are applied as well as international legal documents of varying legal importance.
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Majkic-Singh, Nada. "Society of medical biochemists of Serbia and Montenegro: 50 years anniversary." Jugoslovenska medicinska biohemija 24, no. 3 (2005): 157–70. http://dx.doi.org/10.2298/jmh0503157m.

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Medical biochemistry (synonyms: clinical chemistry or clinical biochemistry) in the terms of professional and scientific discipline, stems from and/or has developed along with the natural sciences and its influences (mathematics, physics, chemistry and biochemistry) and medical sciences as well (physiology, genetics, cell biology). As a scientific discipline, medical biochemistry studies metabolic processes of physiological and pathological changes with humans and animals. Applying analytical chemistry's and biochemistry's techniques enables medical biochemists to gain plenty of information related to diagnosis and prognosis which serve physicians to asses the gravity of illness and prescribe healing therapy. Therefore medical biochemistry is an integral part of modern medicine. This discipline was dubbed various, often confusing names such as pathology, physiology, clinical biology, clinical pathology, chemical pathology, clinical biochemistry, medical biochemistry, clinical chemistry and laboratory medicine, all depending on place of origin. The official, internationally accepted name - clinical chemistry, was mentioned for the first time in 1912 by Johan Scherer, who described his laboratory as Clinical Chemistry Laboratory (Klinisch Chemische Laboratorium) in the hospital Julius in Wurzburg in Germany. After creating national societies of clinical chemists, Professor Earl J. King of Royal Postgraduate Medical School from London incited an initiative to unite national societies into the organization with worldwide character - it was the International Association of Clinical Biochemists, monitored by the International Union for Pure and Applied Chemistry (IUPAC). On 24 July 1952 in Paris, a Second International Congress of Biochemistry was held. A year later, in Stockholm, the name of a newly formed association was altered into International Federation of Clinical Chemistry, which was officially accepted in 1955 in Brussels. Today this federation-s name is International Federation for Clinical Chemistry and Laboratory Medicine (IFCC). Right after the World War II our medical biochemists began to gather within their expert societies. Even before 1950 Pharmaceutical Society of Serbia hosted laboratory experts among whom the most active were Prof. Dr. Aleksandar Damanski for bromatology, Prof. Dr. Momcilo Mokranjac for toxicology and Docent Dr. Pavle Trpinac for biochemistry. When the Managing Board of the Pharmaceutical Society of National Republic of Serbia held its session on 22 December 1950, an issue was raised with reference to creation of a Section that would gather together the laboratory experts. Section for Sanitary Chemistry, combining all three profiles of laboratory staff, i.e. medical biochemists, sanitary chemists and toxicologists, was founded on 1st of January 1951. On 15 May 1955, during the sixth plenum of the Society of Pharmaceutical Societies of Yugoslavia (SFRY) held in Split, the decision was passed to set up a Section for Medical Biochemistry in SFDJ. The Section for Medical Biochemistry in SFDJ was renamed into Society for Medical Biochemistry of SFDJ based on the decision passed during the 16th plenum of SFDJ, held on 15 May 1965 in Banja Luka. Pursuant to the decision passed by SMBY on 6 April 1995 and based on the historic data, 15 May was declared as being the official Day of the Society of Medical Biochemists of Yugoslavia. The purpose of YuSMB (currently SMBSCG) is to gather medical biochemists who would develop and enhance all the branches of medical biochemistry in health industry. Its tasks are as following: to standardize operations in clinical-biochemical laboratories, education of young biochemists on all levels, encouraging scientific research, setting up of working norms and implementation, execution and abiding by the ethics codices with health workers. SMBSCG is to promote the systemized standards in the field of medical biochemistry with the relevant federal and republican institutions. SMBSCG is to enable exchange of experiences of its members with the members of affiliate associations in the country and abroad. .
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Freitas-Rego, F. R., M. G. Pereira, S. O. Loureiro, M. T. de Santana, R. G. Garrido, and F. de S. R. G. Garrido. "Biochemistry: from supermarket to laboratory." Revista de Ensino de Bioquímica 5, no. 2 (May 25, 2007): 21. http://dx.doi.org/10.16923/reb.v5i2.109.

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OOIZUMI, TOORU. "3. Laboratory course of biochemistry." NIPPON SUISAN GAKKAISHI 83, no. 5 (2017): 846. http://dx.doi.org/10.2331/suisan.wa2435-4.

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Saunders, K. B. "Laboratory Instrumentation in Clinical Biochemistry." Journal of the Royal Society of Medicine 91, no. 1 (January 1998): 58–59. http://dx.doi.org/10.1177/014107689809100131.

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Adiga, Usha, and A. Preethika. "Errors in Clinical Biochemistry Laboratory." British Journal of Medicine and Medical Research 14, no. 8 (January 10, 2016): 1–6. http://dx.doi.org/10.9734/bjmmr/2016/25012.

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Boyer, Rodney F. "A proposal for biochemistry laboratory." Biochemical Education 14, no. 1 (January 1986): 12–14. http://dx.doi.org/10.1016/0307-4412(86)90006-3.

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Aksoy, Hilal, Abdullah Ozturk, Dilek Tarhan, Ibrahim Dolukup, and Duygu Ayhan Baser. "Biochemistry laboratory errors and patient safety: Turkey data." Turkish Journal of Biochemistry 46, no. 4 (August 1, 2021): 377–85. http://dx.doi.org/10.1515/tjb-2020-0193.

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Abstract Objectives Our aim in this study is to provide information about the rate of errors in the process of the biochemistry laboratories in the hospitals in Turkey with the “Indicators”. Methods The hospitals calculate their own data according to the indicator cards defined by the Ministry of Health of Turkey and enter into the system once in a year. In this study we examined the quality indicators related to the disruptions in the biochemistry laboratory of hospitals for the year of 2018. Results All indicators except “Non-timely reported result rate in biochemistry laboratory” are found to be significantly higher in university hospitals. This indicator is found to be significantly higher in private hospitals(p:0.030) “Lost sample rate in biochemistry laboratory” is found to be significantly higher in Eastern Anatolia Region (p:0.000) and “Non-timely reported result rate in biochemistry laboratory” is found to be significantly higher in Aegean Region (p:0.008). Conclusions The ratio of non-timely reported result rate is the most seen disruption in biochemistry laboratories. It may be due to lots of reasons; lack of biochemistry equipment, lack of staff, problems in transportation, etc. The management of hospitals and the staff should take measures and regulations about problems.
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Creanga, Iulian, Alexandra Totan, Olivera Lupescu, Iulia-Ioana Stanescu, Costin Dumitru, and Maria Greabu. "Aldolase - From Biochemistry to Laboratory Medicine." Revista de Chimie 70, no. 2 (March 15, 2019): 578–80. http://dx.doi.org/10.37358/rc.19.2.6959.

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Aldolase (ALD) (fructose-1,6-bisphosphate aldolase ) is a 160 kDa, enzyme which catalyzes the conversion of fructose 1-6-biphosphate in glyceraldehyde 3-phosphate and dihydroxyacetone phosphate in the glycolytic metabolic pathway. There are also experimental data suggesting that nuclear ALD isoenzyme A might play an important role in cell proliferation. At the present time, the most useful serum markers of muscle injury following intense, prolonged exercise are: creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase myoglobin and troponin. Although serum ALD is not usually measured yet, it may be used together with CK to evaluate the status of muscle adaptation to training. Recent studies offered ALD a new perspective, as a future valuable biomarker in monitoring the evolution of muscle crush injuries, in order to prevent silent, but progressiv muscle fibers necrosis after injury. It has also been shown that ALD was an independent clinical prognostic marker in many other human cancers, being involved in some well-known signaling pathways.
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Dissertations / Theses on the topic "Biochemistry Laboratory"

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Bradley, Victoria. "Determining sub-arachnoid haemorrhage in the clinical biochemistry laboratory utilising cerebrospinal fluid samples." Thesis, University of Portsmouth, 2013. https://researchportal.port.ac.uk/portal/en/theses/determining-subarachnoid-haemorrhage-in-the-clinical-biochemistry-laboratory-utilising-cerebrospinal-fluid-samples(b68c29d7-afbe-4e20-9c26-a293df652963).html.

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Introduction: Sub-arachnoid haemorrhage (SAH) occurs when cerebral artery ruptures and blood leaks out into the sub-arachnoid space. This is often a catastrophic event for the individual and morbidity and mortality rates are significantly influenced by early intervention. This makes the role of the clinical biochemistry laboratory in early diagnosis vitally important, as delays in diagnosis can have a major clinical impact. The cerebrospinal fluid (CSF) of healthy individuals is optically clear. It has, however, been recognised for over a century that it can become coloured (xanthochromia) following a cerebrovascular incident such as a SAH. This has made the main role of the clinical biochemistry laboratory in SAH diagnosis that of detecting xanthochromia in the CSF. The majority of laboratories which offer a xanthochromia screening service use the national guidelines that are based upon ultra-violet scanning spectrophotometry (350 nm to 600 nm). This analytical technique is not without its problems: it is subjective, has a possibility of inter-operator variability and due to the specialised nature of the test can take many hours or even days for a result to be issued. This project aimed to improve the current laboratory service by investigating: turnaround times, users opinions of the current service and potential alternative analytical methods. Methods: An audit of the current analytical provision was used to assess its effectiveness and in order to elucidate the service users’ perception. This was effected by a questionnaire that was distributed to service users across three different NHS Trusts in England and Wales. In an attempt to improve the laboratory service, alternatives to scanning spectrophotometry were investigated. These were selected through consideration of the nature of SAH i.e. blood is released into the subarachnoid space and the brain is damaged. Laboratory analysis therefore needed to focus on detecting the presence of blood and/or its breakdown products, any change in CSF constituents that arise as a direct consequence of blood being introduced in to the subarachnoid space or a specific analyte which would only be present if brain damage occurred. Investigation of current research into subarachnoid haemorrhage identified the following analytes as potential alternatives: CSF diazo bilirubin, CSF Ferritin, CSF protein S100 and serum protein S100. Results: The audit revealed the average turnaround time for reporting xanthochromia results to be 26 hours, with almost 20% of samples being reported as equivocal. The service user’s questionnaire revealed a general lack of awareness of current United Kingdom National External Quality Assurance Scheme (UKNEQAS) guidelines for the ‘Analysis of cerebrospinal fluid for bilirubin in suspected Subarachnoid haemorrhage’ and a lack of understanding regarding the timing of lumbar punctures. Additionally, one third of users felt that the turnaround time for results was inadequate. CSF protein S100 was found to be unsuitable due to the difficulty in achieving a suitable balance between sensitivity and specificity; at a cut-off of 0.40 μg/l sensitivity is 80% and specificity is 4%, at a cut-off of 1.60 μg/l sensitivity is 40% and specificity is 94%. Serum protein S100 was found to be unsuitable due to the difficulty in achieving a suitable balance between sensitivity and specificity at appropriate cut-offs (66 % and 73%, respectively, at a cut-off of 0.09 μg/l). When the CSF diazo bilirubin and CSF ferritin were compared to current laboratory practises using pre-defined criteria then CSF diazo bilirubin was found to be the analyte of choice to base new guidelines upon. CSF diazo bilirubin was then used as an initial ‘rule-out’ step in a new set of guidelines for the determination of SAH utilising CSF analysis. Conclusion: The new guidelines employ CSF diazo bilirubin analysis as a ‘rule-out’ step with all samples that are above the cut-off (300 nmol/l) being processed through the UKNEQAS guidelines. In order for the guidelines to be introduced and accepted, local training and education programmes for laboratory and clinical staff will need to be developed and implemented and they will need to be disseminated through publication of articles in journals relevant to both the clinical biochemistry community and requesting clinicians.
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De, Wet Tinus Andre. "Laboratory optimization of a protease extraction and purification process from bovine pancreas in preparation for industrial scale up." Thesis, Stellenbosch : Stellenbosch University, 2012. http://hdl.handle.net/10019.1/71790.

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Thesis (MSc)--Stellenbosch University, 2012.
ENGLISH ABSTRACT: This study describes: a) Characterization of traditional methodologies and testing methods used to purify and quantify trypsin and α-chymotrypsin b) Re-engineering / development of a new method for purifying trypsin and α-chymotrypsin that delivered higher product yields and improved control exercised over the process by investigating: i. Extraction methods ii. Centrifugation iii. Ultrafiltration iv. Chymotrypsinogen and trypsin crystallization v. Column chromatography vi. Investigation into different raw material sources for pancreatic enzyme production c) Development of kinetic and ELISA testing methodologies for in-process QC analysis.
AFRIKAANSE OPSOMMING: Hierdie Studie beskryf: a) Karakterisering van die ou prosessering metodes en toets metodes wat gebruik word om Tripsien en Alpha-chimotripsien te suiwer en te kwantifiseer. b) Herontwerp / ontwikkeling van 'n nuwe metode vir die suiwering Tripsien en Chimotripsien wat „n hoër opbrengs lewer en meer kontrole oor die proses uit oefen deur ondersoek in te stel na: i. Ekstraksie- metodes ii. Sentrifugering iii. Ultrafiltrasie iv. Chymotripsienogeen - en tripsien kristallisasie v. Kolom chromatografie vi. Ondersoek na verskillende rou materiaal bronne vir die produksie van pankreas ensieme. c) Die ontwikkeling van kinetiese- en ELISA toets metodes vir die in-proses kwaliteitkontrole.
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Johansson, Isabelle. "The Effect of Contrast Media on Several Common Laboratory Assays." Thesis, Uppsala universitet, Institutionen för kvinnors och barns hälsa, 2018. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-356300.

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Contrast media are commonly used as an enhancement in several diagnostic imaging methods, which in today’s healthcare often are combined with blood works in diagnostics and surgical preparations, as well as to follow up on the patient’s recovery. To save time and money for both the hospital and the patients themselves, the ability to carry out both the radiological examination and the blood works within the same hospital visit would be preferred. However, there have been indications of a potential interference from the contrast media used, and therefore a waiting period is in place. The aim of this study was therefore to see if that waiting period was warranted by testing if contrast media does cause a significant interference in the most common analyses. This was investigated by infusing pooled samples with either iohexol or gadoteric acid, the active components of the most common contrast agents, at either a full dosage or a half dosage. These samples were then run by standard protocol and the results compared to control samples. The results showed that while some analyses proved affected, others proved unaffected or only insignificantly so. Some of the affected analyses were sodium, activated partial thrombin time and hemoglobin. While some analyses such as prostate specific antigen and prothrombin time were unaffected. Analysis of more samples is necessary to confirm the results, but the overall consensus is that while most analyses are unaffected the effects are too large and uncertain to comfortably disregard the waiting time.
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Bagge, Joakim. "Chromatography of Therapeutic Peptides - Contrasting SFC and HPLC." Thesis, Uppsala universitet, Farmakognosi, 2019. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-390890.

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This work is a comparison of a well-established and a novel, "green" and efficient technique to separate peptides of pharmaceutical interest. An attempt is made to derive the chromatographic retention behaviour from these techniques to a number of property descriptors derived from the linear sequence of amino acids. A set of therapeutic peptides were carefully chosen to be experimentally evaluated using in silico-based descriptor calculations. A principle component analysis was performed to assess the distribution of calculated descriptors for including peptides with variable properties. A diluent optimization study was also included to find the optimal diluent for peptides with minimal diluent effects and peak splitting phenomena. The results showed that the solvents tert-butanol and methanol performed best between 20-30 and 50 volumetric percent water as additive in SFC and HPLC, respectively. These diluents were then used for the peptides within the set to evaluate the retention and selectivity in HPLC and SFC. SFC performed well in terms of resolving power. Inparticular, SFC was able to separate Leuprolide and Triptorelin while HPLC was not. A comparison was also made in between the two stationary phases CN and XT, where a global selectivity was shown to be higher for CN. This work does also assess a novel method for determining solubility of analytes in supercritical fluid. The method was evaluated using the pharmaceutical compounds caffeine and aspirin and then used to determine solubility of Leu-Enkephalin in 20% (v/v%) methanol. The solubility of caffeine was determined to be 0.45 mg ml-1 in pure SF-CO2 under 140 bar pressure and 3.9 mg ml-1 for aspirin in 2.4% methanol. Both values correlated well with measurements from four acknowledged papers within this field. Leu-Enkephalin was found to have a solubility of 1.90 mg ml-1 using a solvent corresponding to the initial phase condition of the gradient used for peptide analysis in SFC. Further experimental work is required before the method can be implemented as a useful tool in preparative chromatography, however the results presented here show the compatibility of assessing biomolecules in both pure SF-CO2 and mixed with modifier. The possibility to determine solubility with additional modifier infers an important step of including and evaluating these compounds creating a solid support to subsequent large scale separation.
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Linenberger, Kimberly J. "Biochemistry Students' Understandings of Enzyme-Substrate Interactions as Investigated through Multiple Representations and the Enzyme-Substrate Interactions Concept Inventory." Miami University / OhioLINK, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=miami1321309534.

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Mbajiorgu, Ejikeme Felix. "Interactions of ethanol and chloroquine in the protein-mulnourished male sprague dawley rats : haemotological, biochemical and testicular effects." Thesis, University of Limpopo (Turfloop Campus), 2010. http://hdl.handle.net/10386/751.

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Alvarez, Marina André de. "Modelo de análise do papel das aulas práticas no ensino de bioquímica." Universidade de São Paulo, 2002. http://www.teses.usp.br/teses/disponiveis/46/46131/tde-18062018-093716/.

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Aulas práticas, nesta pesquisa, são aquelas que ocorrem no recinto do laboratório, utilizando vidraria, reagentes, equipamentos e aparelhos especializados. O problema foi como analisá-las de forma a entender suas funções, isto é, o quê elas ensinam, e descobrir seu papel no ensino da disciplina, quer dizer, para que elas ensinam. O objetivo geral deste trabalho foi propor um modelo de análise do papel de aulas práticas no ensino de Bioquímica que desse subsídios ao professor para estabelecer critérios de inclusão e utilização dessas aulas em sua disciplina, considerando-se o contexto em que se inserem. A metodologia teve como base a pesquisa qualitativa, complementada por pesquisa quantitativa, com a visão das Ciências Humanas. A abordagem holística adotada expressou-se na tese defendida no trabalho, que incluiu a idéia de que o contexto das aulas práticas é constituído pela instituição, pelo curso, pela disciplina, pelas condições materiais e de funcionamento do laboratório, pelos professores e pelos alunos, e que os instrumentos de coleta de dados a serem utilizados para a análise de aulas práticas precisam ser abrangentes, precisos, profundos, válidos e confiáveis. Foi produzido um conjunto de documentos intitulado Instrumentos de Coleta de Dados , úteis para levantar informações e fazer observações, tanto para caracterizar o contexto, como para analisar as aulas práticas em si. O uso de parte dos referidos instrumentos também se mostrou adequado para que o corpo técnico das instituições de ensino superior tivesse base para implantar, implementar, manter e gerenciar laboratório de aulas práticas, avaliar planejamentos e trabalho docente. Para complementar, ajustar e julgar a validade dos instrumentos de coleta de dados construídos, eles foram aplicados em seis contextos diferentes, a saber: (1) disciplina de Bioquímica do curso de Ciências Biológicas das Faculdades Integradas Hebraico-Brasileiras Renascença; (2) disciplina de Bioquímica Aplicada do curso de Engenharia Química das Faculdades Oswaldo Cruz; (3) disciplina de Bioquímica do curso de Nutrição do Centro Universitário São Camilo; (4) disciplina de Bioquímica do curso de Nutrição da Faculdade de Saúde Pública; (5) disciplina de Bioquímica Experimental do curso de Farmácia e Bioquímica da Faculdade de Ciências Farmacêuticas; (6) disciplina de Bioquímica do curso de Ciências Biológicas do Instituto de Biociências, os três últimos cursos pertencentes à Universidade de São Paulo. Os seis contextos analisados diferiram pelo tipo de entidade mantenedora e pela classificação das Instituições de Ensino Superior, características previstas pela Lei de Diretrizes e Bases da Educação Nacional em vigor, e por tudo que decorre dessas características. Outras diferenças foram: o enfoque do currículo dos cursos e a posição da disciplina com conteúdo de Bioquímica na grade curricular. Discutiu-se uma forma de estudar aulas práticas, denominada Modelo de Análise do Papel das Aulas Práticas no Ensino de Bioquímica, que permite descobrir quais as funções e definir os papéis destas aulas dentro do contexto em que ocorrem. Servindo-se desse conhecimento, o professor pode estabelecer seus critérios de adoção e utilização do trabalho laboratorial, fundamentar seu planejamento e avaliar o desempenho do ensino prático. De acordo com o modelo de análise proposto, foi realizada uma análise das aulas práticas observadas no seis contextos acima citados, após o levantamento de informações por meio dos instrumentos de coleta de dados, com a forma originalmente concebida, antes das modificações necessárias. Esse estudo serviu de exemplo de como usar o modelo para fazer comparações quanto às características gerais das instituições, com relação a cursos semelhantes, ministrados em instituições pública e privada, e no que se refere aos laboratórios didáticos e ainda para entender o significado das opiniões e do comportamento dos atores do processo ensino-aprendizagem. A análise feita também exemplificou como a definição de funções e de papéis de aulas práticas permite avaliar a adequação das programações das disciplinas com conteúdo de Bioquímica aos cursos em que estão inseridas. As principais conclusões do trabalho foram: • Os Instrumentos de Coleta de Dados criados têm as necessárias abrangência, precisão, profundidade, validez e confiabilidade. • Os referidos instrumentos e o Modelo de Análise do Papel das Aulas Práticas no Ensino de Bioquímica podem ser usados por professores de Bioquímica, por aqueles de outras disciplinas que têm aulas práticas em laboratório e pelo corpo técnico de instituições superiores de ensino, com diferentes fins.
Laboratory classes are those that occur in the laboratory, using specialised glassworks, reagents, equipment and apparatus. The problem is how to analyse those classes in order to understand their functions, that is, what they are teaching, and find their role in the discipline, i. e., for what they are being taught. The general objective of the present work was to propose a model of analysis of laboratory classes role in biochemical education that could offer teachers the basis to establish rules to include and use those classes in their discipline, considering the context where they happen. The methodology applied was founded on qualitative research, complemented with quantitative research, as used in Human Sciences. The thesis defended by the present work had, as a basis, a holistic approach. It was expressed by two assertions: (1) the laboratory classes context is composed of institution, course and discipline characteristics, material conditions and operation of didactic laboratory, teachers and students as persons; (2) the data gathering check lists and questionaires, specially designed to be used for laboratory classes analysis, had to be inclusive, precise, profound, valid and reliable. A set of data gathering documents was specially created to get information and to make observations that are useful to characterise the context and to study the practical work itself. The university institution staffs can apply part of those documents to establish, implement, support and control the didactic laboratory and evaluate teaching planning and work. To complement, adapt and judge the validity of the developed data gathering documents, they were applied at six different contexts, namely: (1) Chemistry of Biomolecules discipline - Biological Sciences course - \"Faculdades Integradas Hebraico-Brasileiras Renascença\"; (2) Biochemistry discipline - Nutrition course - \"Centro Universitário São Camilo\"; (3) Applied Biochemistry discipline - Chemical Engineering course - \"Faculdades Oswaldo Cruz\"; (4) Biochemistry discipline - Nutrition Course - \"Faculdade de Saúde Púclica - USP\"; (5) Experimental Biochemistry discipline - Pharmaceutical Sciences course - \"Faculdade de Ciências Farmacêuticas - USP\"; (6) Biochemistry discipline - Biological Sciences course - \"Instituto de Biociências - USP\". The six contexts analysed differed with regard to maintainer corporation type and position in the university institution classification, in accordance with the \"Lei de Diretrizes e Bases da Educação Nacional\" ( National Education Guidelines and Basis Law) in force. Other differences were: curriculum focus and the order of discipline with biochemistry contents in discipline roll of the course. A laboratory class kind of study was discussed. It was called \"A model of analysis of laboratory classes role in biochemical education\". This model allows to disclose the functions and to define the roles of laboratory classes in the context where they happen. Using it, the teachers can establish their criteria of adoption and utilisation of laboratory work, they can base their planning and evaluate their performance in practical teaching. In accordance with the proposed model, an analysis of the observed laboratory classes in the six contexts cited before was carried out. It was applied to obtain information, the original data gathering documents, as they were prior conceived. The developed study helped to exemplify how to use this model to compare general characteristics of university institutions, analogous courses of public and private colleges, material and operation conditions of didactic laboratories. It also helped to understand the opinions and behaviours of the instruction-apprenticeship process actors, i. e., teachers and pupils. This analysis can also be used as an example of how the definition of laboratory classes functions and roles permits to evaluate the adequacy of Biochemistry discipline programs to the courses in where they are inserted. The main conclusions of this work were: • The elaborated data gathering documents have the necessary richness, profoundness, validity and trustworthiness. • The documents and the model of analysis of laboratory classes role in biochemical education can be used by Biochemistry teachers, by teachers of other disciplines that have laboratory classes and by other members of university institution staff, to several purposes.
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Zhang, Wei. "Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis." Doctoral thesis, Uppsala universitet, Biokemi, 2011. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-158400.

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Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. Is it a one-way track that protein practitioners can only learn from nature to enable more efficient protein engineering? A structure-and-mechanism-based approach, supplemented with the use of reduced amino acid alphabets, was proposed as a general means for semi-rational enzyme engineering. Using human GST A2-2*E, the most active human enzyme in the bioactivation of azathioprine, as a parental enzyme to test this approach, a L107G/L108D/F222H triple-point mutant of GST A2-2*E (thereafter designated as GDH) was discovered with 70-fold increased activity, approaching the upper limit of specific activity of the GST scaffold. The approach was further experimentally verified to be more successful than intuitively choosing active-site residues in proximity to the bound substrate for the improvement of enzyme performance. By constructing all intermediates along all putative mutational paths leading from GST A2-2*E to mutant GDH and assaying them with nine alternative substrates, the fitness landscapes were found to be “rugged” in differential fashions in substrate-activity space. The multidimensional fitness landscapes stemming from functional promiscuity can lead to alternative outcomes with enzymes optimized for other features than the selectable markers that were relevant at the origin of the evolutionary process. The results in this thesis suggest that in this manner an evolutionary response to changing environmental conditions can readily be mounted. In summary, the thesis demonstrates the attractive features of the structure-and-mechanism-based semi-rational directed evolution approach for optimizing enzyme performance. Moreover, the results gained from the studies show that laboratory evolution may refine our understanding of evolutionary process in nature.
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Asuru, Awuri P. "Applications of X-ray Hydroxyl Radical Protein Footprinting." Case Western Reserve University School of Graduate Studies / OhioLINK, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=case1575877091577049.

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Serrano, Moises A. "Novel Roles of Replication Protein A Phosphorylation in Cellular Response to DNA Damage." Digital Commons @ East Tennessee State University, 2013. https://dc.etsu.edu/etd/1206.

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Human replication protein A (RPA) is an eukaryotic single-stranded DNA binding protein directly involved in a variety of DNA metabolic pathways including replication, recombination, DNA damage checkpoints and signaling, as well as all DNA repair pathways. This project presents 2 novel roles of RPA in the cellular response to DNA damage. The first elucidates the regulation of RPA and p53 interaction by DNA-dependent protein kinase (DNA-PK), ataxia telangiectasia mutated (ATM) and ATM- and Rad3-related (ATR) in homologous recombination (HR). HR and nonhomologous end joining (NHEJ) are 2 distinct DNA double-stranded break (DSB) repair pathways. Here, we report that DNA-PK, the core component of NHEJ, partners with DNA-damage checkpoint kinases ATM, and ATR to synergistically regulate HR repair of DSBs. The regulation was accomplished through modulation of the p53-RPA interaction. We show that upon DNA damage p53 and RPA are freed from the p53–RPA complex. This is done through simultaneous phosphorylation of RPA by DNA-PK, and p53 by ATR and ATM. Neither the phosphorylation of RPA nor that of p53 alone could dissociate the p53-RPA complex; furthermore, disruption of the release significantly compromised HR repair of DSBs. Our results reveal a mechanism for the crosstalk between HR and NHEJ repair through the coregulation of p53–RPA interaction by DNA-PK, ATM and ATR. The second part of this project reveals a novel role of RPA32 phosphorylation in suppressing the signaling of programmed cell death, also known as apoptosis. Our results show that deficiency in RPA32 phosphorylation leads to increased apoptosis after genotoxic stress. Specifically, PARP-1 cleavage, Caspase-3 activation, sub-G1 cell population, annexin V staining and the loss of mitochondrial membrane potential were significantly increased in the phospho-deficient RPA32 cells (PD-RPA32). The lack of RPA phosphorylation also promoted activation of initiator Caspase-9 and effector Caspase-3 and -7. This regulation is dependent on the kinase activity of DNA-PK and is mediated by PUMA through the ATM-p53 pathway. Our results suggest a novel role of RPA phosphorylation in apoptosis that illuminates a new target that lies on the crossroads of DNA repair and cell death, a pivotal point that could be of importance for sensitizing cancer cells to chemotherapy.
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Books on the topic "Biochemistry Laboratory"

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Laboratory developments in paediatric biochemistry, proceedings of a symposium (26th April 1989 Liverpool). Laboratory developments inpaediatric biochemistry. Liverpool: Institute of medical laboratory sciences, 1989.

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Stroev, E. A. Laboratory manual in biochemistry. Moscow: Mir, 1989.

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An introduction to practical biochemistry. 3rd ed. London: McGraw-Hill, 1987.

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F, Lata Gene, ed. Experimental biochemistry. New York: Oxford University Press, 1989.

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Biochemistry laboratory: Modern theory and techniques. 2nd ed. Upper Saddle River, N.J: Pearson Prentice Hall, 2012.

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Boyer, Rodney F. Biochemistry laboratory: Modern theories and techniques. San Francisco: Benjamin Cummings, 2006.

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Bettelheim, Frederick A. Laboratory experiments for general, organic & biochemistry. 3rd ed. Fort Worth [Tex.]: Saunders College, 1995.

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Bettelheim, Frederick A. Laboratory experiments for General, organic & biochemistry. Fort Worth: Saunders College Pub., 1995.

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Bettelheim, Frederick A. Laboratory experiments for general, organic & biochemistry. 3rd ed. Forth Worth: Saunder College Pub., 1997.

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Bettelheim, Frederick A. Laboratory experiments for general, organic & biochemistry. 2nd ed. Fort Worth [Tex.]: Saunders College, 1995.

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Book chapters on the topic "Biochemistry Laboratory"

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von Beust, Barbara R., and Gregory S. Travlos. "Biochemistry of Immunoglobulins." In The Clinical Chemistry of Laboratory Animals, 551–86. Third edition. | Boca Raton : Taylor & Francis, 2017.: CRC Press, 2017. http://dx.doi.org/10.1201/9781315155807-16.

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Ramakrishnan, S., and KN Sulochana. "Biochemistry." In Manual of Medical Laboratory Techniques, 1. Jaypee Brothers Medical Publishers (P) Ltd., 2012. http://dx.doi.org/10.5005/jp/books/11559_1.

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McGhee, Michael. "Biochemistry." In A Guide to Laboratory Investigations, 1–2. CRC Press, 2019. http://dx.doi.org/10.1201/9781846199400-6.

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Gupte, Satish. "Biochemistry." In The Short Textbook of Medical Laboratory for Technicians, 207. Jaypee Brothers Medical Publishers (P) Ltd., 2014. http://dx.doi.org/10.5005/jp/books/12106_8.

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Chhabra, Namrata, and Sahil Chhabra. "Laboratory Glassware." In Handbook of Biochemistry Spotting, 1. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12856_2.

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Chhabra, Namrata, and Sahil Chhabra. "Laboratory Equipment." In Handbook of Biochemistry Spotting, 11. Jaypee Brothers Medical Publishers (P) Ltd., 2016. http://dx.doi.org/10.5005/jp/books/12856_3.

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Ayling, Ruth M. "The clinical biochemistry laboratory." In Rennie & Roberton's Textbook of Neonatology, 1239–42. Elsevier, 2012. http://dx.doi.org/10.1016/b978-0-7020-3479-4.00043-x.

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"Common Laboratory Values." In Rapid Review Biochemistry, 161–63. Elsevier, 2011. http://dx.doi.org/10.1016/b978-0-323-06887-1.00014-8.

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Naik, Pankaja. "Laboratory Investigation Techniques." In Essentials of Biochemistry, 462. Jaypee Brothers Medical Publishers (P) Ltd., 2017. http://dx.doi.org/10.5005/jp/books/12934_36.

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MK, Ganesh, Shivaraja YM, and Shivashankara AR. "Laboratory Equipments, Glassware, Laboratory Hazards and General Laboratory Rules." In Laboratory Manual for Practical Biochemistry, 1. Jaypee Brothers Medical Publishers (P) Ltd., 2013. http://dx.doi.org/10.5005/jp/books/12009_1.

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Conference papers on the topic "Biochemistry Laboratory"

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Navarro Llorens, Juana Maria, Ana Saborido Modia, Miguel Arroyo Sánchez, Mar Lorente Pérez, Regina Ranz Valdecasa, Teresa López Conejo, Jose Luis Nieto Bueno, et al. "NEW CHALLENGES IN THE BIOCHEMISTRY LABORATORY: CONNECTING SECOND-YEAR STUDENTS OF BIOCHEMISTRY DEGREE TO LABORATORY PRACTICE." In 12th International Technology, Education and Development Conference. IATED, 2018. http://dx.doi.org/10.21125/inted.2018.2192.

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Pérez-Mercader, Juan. "De novo laboratory synthesis of life mimics without biochemistry." In The 2020 Conference on Artificial Life. Cambridge, MA: MIT Press, 2020. http://dx.doi.org/10.1162/isal_a_00282.

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Craig, Norman. "RAMAN INVESTIGATION OF TEMPERATURE PROFILES OF PHOSPHOLIPID DISPERSIONS IN THE BIOCHEMISTRY LABORATORY." In 70th International Symposium on Molecular Spectroscopy. Urbana, Illinois: University of Illinois at Urbana-Champaign, 2015. http://dx.doi.org/10.15278/isms.2015.tc09.

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Ye, Liang, Nai Sum Wong, and Joanna Wen Ying Ho. "Development of a Prototype Biochemistry Virtual Laboratory: Reflections on the Instructional Design." In Annual International Conference on Education & e-Learning. Global Science & Technology Forum (GSTF), 2015. http://dx.doi.org/10.5176/2251-1814_eel15.30.

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Anwar, Yunita Arian Sani, Senam, and Endang W. Laksono. "Identification of the students’ critical thinking skills through biochemistry laboratory work report." In THE 4TH INTERNATIONAL CONFERENCE ON RESEARCH, IMPLEMENTATION, AND EDUCATION OF MATHEMATICS AND SCIENCE (4TH ICRIEMS): Research and Education for Developing Scientific Attitude in Sciences And Mathematics. Author(s), 2017. http://dx.doi.org/10.1063/1.4995112.

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Saborido, Ana, M. Isabel de la Mata Riesco, Bárbara Olmeda Lozano, Mar Lorente Pérez, Miguel Arroyo Sánchez, Maria José Feito Castellano, Antonio Sánchez Torralba, and Juana María Navarro Llorens. "STRATEGIES TO INVOLVE THE STUDENTS IN THEIR LEARNING IN A BIOCHEMISTRY LABORATORY." In 15th International Technology, Education and Development Conference. IATED, 2021. http://dx.doi.org/10.21125/inted.2021.0397.

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Ferreira, Rui Manuel Alves, Isabel Maria Simão Alves-Pereira, Joana Manuela Capela-Pires, and Marta Sofia Garcia Candeias. "Functional and conservation value of fruits - a lab approach." In Sixth International Conference on Higher Education Advances. Valencia: Universitat Politècnica de València, 2020. http://dx.doi.org/10.4995/head20.2020.11082.

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Fruits are a relevant source of phenols and ascorbate, biomolecules which scavenge reactive oxygen species. For this reason, they are considered as healthy for the human being. Fruits quality depends on their levels of antioxidants and enzyme activities that ensure their conservation. The aim of this work was to plan and execute a laboratory class of Enzymology, a discipline of Biochemistry degree of University of Évora, Portugal, for determining the functional and conservation value of three different fruits types, sold in the market of Évora, Portugal. The development of this activity allowed that students of a pilot class participate in a laboratory activity which intended to compare the content of phenols, ascorbate, and polyphenol oxidase enzyme activity present in apple, peach and blueberries pulp. At Lab activity, the students successfully determined markers of functional and conservation value of selected fruits. The skills acquired by the students, in terms of obtaining fruit pulp and their composition in antioxidants, stimulated their commitment degree on the application of biochemistry in the everyday, acquiring thereby significant learning, with a high degree of satisfaction.
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Hosseini, Saeedeh, Jacqueline Vartanoosian, and Fatemeh Hosseini. "COMPARING OSCE/OSPE SCORES OF BIOCHEMISTRY LABORATORY IN MALE AND FEMALE NURSING STUDENTS AT SHAHID BEHESHTI UNIVERSITY OF MEDICAL SCIENCES FROM 2011 TO 2015." In International Conference on Education and New Learning Technologies. IATED, 2017. http://dx.doi.org/10.21125/edulearn.2017.0108.

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Ugolini, Daniele, Francesco Rossi, and Francesco Basile. "Decommissioning of the Radio Chemical Hot Laboratory of the European Commission Joint Research Centre of Ispra." In ASME 2011 14th International Conference on Environmental Remediation and Radioactive Waste Management. ASMEDC, 2011. http://dx.doi.org/10.1115/icem2011-59207.

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The construction of the Radio Chemical Hot Laboratory (RCHL) of the Joint Research Centre (JRC) of Ispra began in the early 1960s while the laboratory activities started in 1964. In 1976 an annex to the main building was built. At this time the RCHL main research activities were in environment and biochemistry by means of radioactive tracers; neutron activation analyses; extraction of actinides from radioactive liquid waste coming from the nuclear fuel reprocessing plants; and analyses of U, Pu, and Th in samples from the nuclear fuel cycle in order to determine the isotopic ratio and the burn-up. In 1978, a new area of laboratories named “Stabularium” was built to study the metabolism of heavy metal on laboratory animals. Complementary to the laboratory three pneumatic transfer systems for irradiated sources connected the RCHL to two research reactors. The decommissioning activities of the 2650 m2 facility started in January 2008 and they were completed at the end of 2010 with the release for unrestricted use of all the buildings of the facility. They consisted in five main tasks; pre-decommissioning, licensing, dismantling, waste management, and final survey. The main pre-decommissioning activities were the physical and radiological characterization of the facility. The principal licensing activity was the preparation of the delicensing documentation to obtain the license termination from the safety authorities. Dismantling consisted in the removal of all the equipments and ancillary systems, of the pneumatic transfer system, and in the decontamination of the structures of the controlled zone. The waste management was limited to the transfer of the waste and of the clearable material to the centralized waste management facility. The final survey consisted in the final radiological characterization to quantify the concentration of any residual radioactivity remained after the completion of the dismantling activities for the release of the RCHL without any radiological constraints. The safety and radioprotection prescriptions adopted were the minimization of the conventional and nuclear risk for the workers (reducing the dose rate), the minimization of the environmental risks (reducing the external liquid, solid and gaseous releases), and the confinement of the contamination where it was generated. This paper describes the pre-decommissioning, dismantling, and final survey activities undertaken to perform the decommissioning of the RCHL.
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