Dissertations / Theses: 'Biochemistry of proteins'

1

Bateman, Libei. "Studies of heme proteins using protein film voltammetry." Thesis, University of Oxford, 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.289588.

Full text

APA, Harvard, Vancouver, ISO, and other styles

2

Skerjanc, Ilona S. "Import of proteins into Mitochondria : properties of precursor proteins." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74254.

Full text

Abstract:

The mechanism of protein translocation into mitochondria has been investigated by studying properties of precursor proteins destined for the mitochondrial matrix. Characterization of the amphiphilic properties of the signal sequence for pre-ornithine carbamyltransferase has led to the conclusion that precursors can not translocate across the inner membrane via a lipid route alone (i.e. in the absence of proteins). A correlation was established between the rate of precursor import and the degree of hydrophobicity of a short region in the presequence, suggesting that precursor binding to the two-dimensional phospholipid surface of the outer membrane may enhance the rate of diffusion to the translocation apparatus. The conformations of the mature portions of two hybrid proteins, pOCAT and pODHFR, were examined at various steps on the import pathway. The bulk population of these precursors remained in a near-native conformation prior to precursor engagement of the import apparatus. Unfolded polypeptide translocation intermediates, the formation of which requires ATP, an intact signal sequence, and a protease-sensitive component of the outer mitochondrial membrane, have been detected in association with submitochondrial fractions containing sites of contact between the inner and outer mitochondrial membranes.

APA, Harvard, Vancouver, ISO, and other styles

3

McLoughlin, D. M. "Identification of proteins interacting with the Alzheimer's disease amyloid precursor protein." Thesis, King's College London (University of London), 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.343724.

Full text

APA, Harvard, Vancouver, ISO, and other styles

4

Mace, Peter, and n/a. "Biochemistry of ovine bone and morphogenetic proteins and receptors." University of Otago. Department of Biochemistry, 2006. http://adt.otago.ac.nz./public/adt-NZDU20070508.133410.

Full text

Abstract:

The transforming growth factor (TGF)-β superfamily mediates a wide range of differentiation and developmental processes across many genera. GDF9 and BMP15 are expressed exclusively in the mammalian ovary and are the only TGF-β ligands that lack the conserved cysteine residue used for dimerisation. As a platform for studying the interactions between GDF9 and BMP15 and their receptors, BMPRII and BMPRIb, a variety of strategies were attempted to produce soluble and active proteins from recombinant systems. Both ligands and receptors showed a tendency to form insoluble aggregates when expressed in prokaryotic systems; however after extensive screening, quantities of biologically active GDF9 were produced using in vitro refolding. When expressed alone, either containing a histidine tag or as an untagged protein, the BMPRII ectodomain was deposited as insoluble inclusion bodies. This protein, subjected to in vitro refolding procedures, exhibited multiple species following anion exchange chromatography and size exclusion chromatography, as visualised on native PAGE. Separation of these species could be achieved using a MonoP matrix. One of these separated fractions, representing about 5% of the starting material, was amenable to crystallisation, and furthermore exhibited activity in a rat granulosa cell thymidine incorporation assay. Two different crystals forms of the extracellular domain of BMPRII were grown from the same protein batch under similar crystallisation conditions. Notably, the tetragonal form that grew more slowly possessed several disordered finger regions, while electron density for the entire molecule was clear in the orthorhombic form. The hydrophobic core of the ligand binding surface of BMPRII , as seen in both structures, resembles that of ActRII bound to BMP2. The A-loop of BMPRII, which is involved in ligand binding, lies in two different conformations in the two structures of BMPRII, mediated by a rearrangement in disulfide Cys94-Cys117. It is proposed here that the tetragonal form represents the ligand-bound receptor structure. Although the majority of the hydrophobic binding surface is shared with ActRII(b), it is likely that His87 and Tyr40 are unique residues that confer specificity in BMPRII ligand binding.

APA, Harvard, Vancouver, ISO, and other styles

5

Reid, Elizabeth A. "The chemistry and biochemistry of lysine residues in proteins." Thesis, University of Canterbury. Chemistry, 2004. http://hdl.handle.net/10092/6641.

Full text

Abstract:

This thesis investigated the nutritional consequences of processing chicken feed, in particular the loss of available amino acids as a result of the Maillard reaction, both in model protein systems and during poultry feed processing. Model systems containing RNase A and pure or feed-type carbohydrates were incubated at 37°C, 50°C or 70°C for up to 8 days. From lysine analysis, reduction in reactive amino groups in RNase A was shown to occur rapidly at room temperature, with no incubation period necessary. However, protein fragmentation that occurred during incubation, especially at higher temperatures, interfered with the quantification of lysine. SDS-PAGE showed crosslinking reactions of RNase A with the tested carbohydrates to be slow below 70°C. Incubation of RNase A with cyclotene or xylose produced the greatest rate of crosslinking, while starch and sucrose produced the least. A modified OPA method was developed such that the level of reactive lysine could be quantitated in barley flour proteins, in a manner that was technically straightforward, inexpensive and allowed good through-put of samples. This method was shown to have good agreement with the published ninhydrin method in the measurement of Maillard reacted lysine in barley flours. Up to 25% loss of reactive lysine was observed in barley flour that had not undergone processing beyond milling. Samples were taken before, during and after the pelleting of chicken feed. Lysine analysis of these samples showed loss in amino group content of at least 18% could occur during processing, although this was dependent on variations between pelleting runs. Protein fragmentation during processing potentially masked further losses. A growth trial assessed a novel method of lysine addition to the feed, via spraying on free lysine solution post-pelleting. Over the 2 week trial period, 10% of the lysine applied by this method remained in the uneaten fines. Lysine eaten by chickens aged day 8-14, as calculated from measurements taken using the OPA method, correlated well with bird growth. Increased feed intake was also seen as the lysine content of the diet increased. In chickens aged day 15-21, lysine addition above the first addition level produced no further significant gain in performance. Therefore, lysine was not growth limiting above this level. Results indicated that over the 2 week period of the trial, equivalent performance for birds on the standard feed could have been achieved with a 50% reduction in free lysine added to the feed formulation. No lysine loss occurred in the standard sample as a result of pelleting. Therefore, while the potential exists for lysine loss to occur during chicken feed pelleting, this thesis has shown that this is not a significant problem. As up to 25% lysine blockage in unprocessed barley flour was observed, obtaining feed ingredients with consistently low levels of Maillard reaction damage may be as important as maintaining ideal processing conditions.

APA, Harvard, Vancouver, ISO, and other styles

6

Yoshimune, Kazuaki. "Studies on biochemistry and application of Hsp70 family proteins." Kyoto University, 2005. http://hdl.handle.net/2433/144991.

Full text

Abstract:

Kyoto University (京都大学) 0048 新制・論文博士 博士(農学) 乙第11672号 論農博第2555号 新制||農||912(附属図書館) 学位論文||H17||N4055(農学部図書室) 23485 UT51-2005-D590 京都大学大学院農学研究科応用生命科学専攻 (主査)教授江﨑信芳, 教授喜多恵子, 教授植田充美 学位規則第4条第2項該当

APA, Harvard, Vancouver, ISO, and other styles

7

Fernig, D. "Intracellular degradation of nuclear proteins : Studies on transplanted B82 karyoplast proteins." Thesis, University of Nottingham, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.355289.

Full text

APA, Harvard, Vancouver, ISO, and other styles

8

Ellis, Matthew James. "Electron crystallography of soluble proteins /." Stockholm, 1999. http://diss.kib.ki.se/1999/91-628-3549-1/.

Full text

APA, Harvard, Vancouver, ISO, and other styles

9

Handoll, H. H. G. "Crystallographic studies of proteins." Thesis, University of Oxford, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.370263.

Full text

APA, Harvard, Vancouver, ISO, and other styles

10

Chappell, David Clive. "Fluorescent labelling of proteins." Thesis, City University London, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292598.

Full text

APA, Harvard, Vancouver, ISO, and other styles

11

Nadassy, Katalin. "Molecular recognition by proteins : structural features of zinc and protein-nucleic acid binding sites." Thesis, University of Stirling, 2000. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.341227.

Full text

APA, Harvard, Vancouver, ISO, and other styles

12

Doak, David G. "Peptide models of transmembrane proteins." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.359445.

Full text

APA, Harvard, Vancouver, ISO, and other styles

13

Teuten, Andrew J. "NMR studies of fibrinolytic proteins." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.303639.

Full text

APA, Harvard, Vancouver, ISO, and other styles

14

Smith, Linda J. "Structural studies of adsorbed proteins." Thesis, University of East Anglia, 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.320864.

Full text

APA, Harvard, Vancouver, ISO, and other styles

15

Sousa, Isabel Maria Nunes de. "Functional properties of lupin proteins." Thesis, University of Nottingham, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.385965.

Full text

APA, Harvard, Vancouver, ISO, and other styles

16

Goulding, Paul Nicholas. "Interactions between proteins and polyphenols." Thesis, University of Sheffield, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.388059.

Full text

APA, Harvard, Vancouver, ISO, and other styles

17

Ariss, A. J. "Immunocytochemical studies of wheat proteins." Thesis, University of Hertfordshire, 1986. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.371392.

Full text

APA, Harvard, Vancouver, ISO, and other styles

18

Hutnik, Cindy Mary-Lynn. "The conformational heterogeneity of proteins." Thesis, University of Ottawa (Canada), 1990. http://hdl.handle.net/10393/5844.

Full text

Abstract:

Time-correlated single photon counting and steady-state fluorescence spectroscopy were used to investigate the conformational properties of select proteins. Specifically, the role of naturally associated metal ions in modulating the conformation of members from two families of metalloproteins was examined. Two homologous bacterial copper-containing azurins were purified from Pseudomonas fluorescens (ATCC 13525) and Pseudomonas aeruginosa (ATCC 10145). The intrinsic fluorescence of the native Cu(II) single tryptophan-containing protein, as well as experimentally prepared Cu(I), Ni(II), Co(II) and metal-free derivatives demonstrated that the metal centre of these redox proteins played an important role in the conformational heterogeneity of the protein. Such an effect was strongly dependent on the nature of the metal ion. From the calcium-binding superfamily, the single-tryptophan containing isotype III component of parvalbumin was purified from codfish. The previous notion that this protein was a Ca(II)/Mg(II)-specific protein was shown to be due to an experimental artefact arising from the use of the soluble chelator EGTA. The Ca(II)-specific conformational changes of cod III parvalbumin were compared with those of the highly homologous Ca(II)-binding tumour protein oncomodulin. Since native tumour oncomodulin was devoid of tryptophan, a site-specific mutant of oncomodulin with tryptophan in the identical position of the parvalbumin tryptophan was examined. The results showed that the ability of oncomodulin to function as a modulator (unlike parvalbumin) may be due to relatively subtle Ca(II)-specific conformational changes. Details of these changes were obtained by an examination of a number of oncomodulin mutant proteins in which the non-fluorescent phenylalanine, and the highly fluorescent tryptophan had been substituted into various positions of the two Ca(II)-binding loops. The results demonstrated that the binding of the first equivalent of Ca(II) induced greater than 90% of the conformational changes experienced by various probed positions, but that the second equivalent of Ca(II) played an important role in orienting the two Ca(II)-binding loops relative to each other.

APA, Harvard, Vancouver, ISO, and other styles

19

Liu, Xingquan 1959. "Import of proteins into mitochondria : biogenesis of the uncoupling protein and identification of a mitochondrial signal peptide binding protein." Thesis, McGill University, 1989. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=74310.

Full text

Abstract:

The inner membrane uncoupling protein (UCP) of rat brown fat mitochondria has been imported into rat heart mitochondria in vitro. Two import signals have been detected in UCP. The intrinsic membrane insertion information of UCP has been abrogated by a signal sequence fused in front of UCP, resulting in the rerouting of UCP into the matrix. Following removal of the signal sequence from the hybrid protein, the UCP moiety remained in the soluble matrix space indicating an incompatibility of UCP insertion into the inner membrane from the matrix side. An integral mitochondrial membrane protein (p30) that binds a mitochondrial signal peptide in intact mitochondria in vitro has been purified by an affinity approach. The protein has been identified as a member of the ADP/ATP carrier (AAC) family based on both immunoblotting and peptide mapping. The irreversible association of the signal peptide with AAC in intact mitochondria has been correlated with inhibition of protein import into the organelle.

APA, Harvard, Vancouver, ISO, and other styles

20

Bulheller, Benjamin M. "Circular and linear dichroism spectroscopy of proteins." Thesis, University of Nottingham, 2009. http://eprints.nottingham.ac.uk/10866/.

Full text

Abstract:

Circular dichroism (CD) is an important technique in the structural characterization of proteins, and especially for secondary structure determination. The CD of proteins can be calculated from first principles using the matrix method, with an accuracy that is almost quantitative for helical proteins. Thus, for proteins of unknown structure, CD calculations and experimental data can be used in conjunction to aid structure analysis. The vacuum-UV region (below 190 nm), where charge-transfer transitions have an influence on the CD spectra, can be accessed using synchrotron radiation circular dichroism (SRCD) spectroscopy. Calculations of the vacuum-UV CD spectra have been performed for 71 proteins, for which experimental SRCD spectra and X-ray crystal structures are available. The theoretical spectra are calculated considering charge-transfer and side chain transitions, which significantly improves the agreement with experiment, raising the Spearman correlation coefficient between the calculated and experimental intensity at 175 nm from 0.12 to 0.79. The influence of the different conformations used for the calculation of charge-transfer transitions is discussed in detail, focussing on the effect in the vacuum-UV. Linear dichroism (LD) provides information on the orientation of molecules but is more challenging to analyze than CD. To aid the interpretation of LD spectra, the calculation of protein LD using the matrix method is established and the results compared to experimental data. The orientations of five prototypical proteins are correctly reproduced by the calculations. Using a simplified approach, matrix method parameter sets for the nucleic bases and naphthalenediimide (NDI) have been created and are used to determine DNA/RNA conformations and to study NDI nanotubes. Finally, to make CD and LD calculations available for the scientific community in an easy-to-use fashion, the web interface DichroCalc is introduced.

APA, Harvard, Vancouver, ISO, and other styles

21

Bryan, Steven. "Rho/Rac GTP-binding proteins and their GTPase activating proteins in humans and in Dictyostelium discoideum." Thesis, University College London (University of London), 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.266164.

Full text

APA, Harvard, Vancouver, ISO, and other styles

22

Zhang, Yun-Heng. "Biochemistry and molecular biology of binding proteins for plant growth regulators." Thesis, De Montfort University, 2000. http://hdl.handle.net/2086/13254.

Full text

Abstract:

Plant growth regulators have a vital role in plant growth and development. The cellular response to these regulators depends on the presence and the action of specific receptors. The plant growth regulators and their receptors act together in complexes which determine the final effects of the plant growth regulators. In the research reported here, emphasis has been given to the regulation of the activity of the receptors themselves. The regulation of the N-l-naphthylphthalamic acid (N~A) receptor through phosphorylation and dephosphorylation and the regulation of the auxin binding protein (ABP) through gene manipulation have been investigated. NPA, an auxin transport inhibitor, was found to bind specifically to a crude membrane preparation from sugar beet seedling leaf cell suspension cultures. The in vitro binding was optimal at pH 4.5 and 4?C. Binding parameters for NP A binding were determined by Scatchard analysis. The dissociation constant (Kd) and binding protein concentration were found to be 1.71 x 10-7 mol dm-3 and 220 pmoles g-I membrane protein respectively. It was found that the amount of specific 3H-NPA binding was significantly increased by adding Mg2+ A TP to the binding assay solution; treatment of membrane preparations with acid phosphatase, prior to the NP A binding assay, resulted in lower specific binding. A TP activation and phosphatase inactivation were culture stage dependent. Although a considerable effect could be detected when using cells from day 8 (representing the linear phase), the same treatment did not alter the binding if cells from day I (representing lag phase) or day 14 (representing the stationary phase) were used. These observations have strongly highlighted the possible involvement of a phosphorylation and dephosphorylation mechanism in vivo in the regulation of the activity of the NP A receptor. High phosphatase activity was found in the supernatant, but not in the membrane pellet, after 50,000 g centrifugation. The presence of a membrane-bound auxin receptor, ABP, was demonstrated by Scatchard analysis in sugar beet seedlings. The Kd value and the receptor concentration were found to be 2.15 x 10-6 mol dm-3 and 68 pmoles g-I membrane protein. The protein could be solubilised either with the detergent Triton X-I 00 or by acetone-washing, with a recovery of about 40%. An acetone-solubilised ABP preparation could be partially purified by DEAE-Sephacel ion exchange chromatography, NAA-linked AH-Sepharose 4B affmity chromatography or Sephadex G-200 gel filtration. The recovery after any of these chromatographic treatments was very low so that successive chromatography for further purification was unsuccessful. The low level of detectable binding after purification resulted mainly from the low abundance of ABP in the plant material. Non-radioactive labelling and detection techniques were used to show that an ABP-probe hybridized to sugar beet genomic DNA during dot blotting. The present study has indicated that receptor activity could be regulated by a phosphorylation and dephosphorylation mechanism in plants. The investigation has also suggested that the effect of plant growth regulators on plant development could be regulated through the manipulation of the expression of their receptor genes.

APA, Harvard, Vancouver, ISO, and other styles

23

Dennis, Caitriona Anne. "Structural studies on colicin E immunity proteins and di-sulphide binding protein A from Vibrio cholerae." Thesis, University of East Anglia, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.267320.

Full text

APA, Harvard, Vancouver, ISO, and other styles

24

Nguyen, Mai. "Topogenic sequences of mitochondrial precursor proteins." Thesis, McGill University, 1986. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=75420.

Full text

Abstract:

A cDNA encoding the precursor form of rat liver mitochondrial matrix enzyme, ornithine carbamyl transferase (pOCT) was cloned and its complete nucleotide sequence was determined. Full-length pOCT cDNA was cloned in pSP64 and expressed under the control of the SP6 promoter. The SP6-produced precursor protein was imported by rat heart mitochondria in vitro and processed to mature protein. Fusions of pOCT presequence to bacterial asparagine synthetase (AS) facilitated delivery of the hybrid protein to the mitochondrial matrix, indicating that the signal sequence of precursor ornithine carbamyl transferase contains sufficient information to target and translocate attached proteins into mitochondria. Deletion mutation within the pOCT signal sequence (removal of residues 22-30) resulted in a mutant precursor which was imported into the mitochondrial matrix, but remained largely unprocessed; furthermore, the low level of processing that was observed appears to be incorrect. Deletion and substitution mutation introduced to the downstream region of the normal pOCT processing site affected neither import nor correct processing. Fusion of pOCT amino-terminal region to the vesicular stomatitis G protein (VSV G), an integral protein localized to the plasma membrane of infected cells, allows post-translational insertion as well as proteolytic processing of the hybrid protein. The protein, however, remained anchored at the mitochondrial inner membrane, apparently via the hydrophobic VSV G stop-transfer domain. Taken together, the results help to elucidate the nature of signal domains within mitochondrial precursor proteins that specify targeting, membrane-translocation, translocation arrest, intra-mitochondrial sorting, and processing-site recognition.

APA, Harvard, Vancouver, ISO, and other styles

25

Morrison, Megan. "Studies on adenovirus E4orF6 binding proteins." Thesis, McGill University, 2000. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=30824.

Full text

Abstract:

The adenovirus type 5 E4orf6 protein plays many critical roles in the viral lifecycle. This thesis attempts to identify three binding partners of E4orf6: p14, p19, and pp84. Mapping studies revealed residues 1--43 of E4orf6 were unnecessary for p14 and p19 binding. Mdm-2 and p14 ARF were selected as potential candidates for pp84 and p19 respectively. No interaction was observed between E4orf6 and Mdm-2. Binding of E4orf6 and p14ARF was observed, however attempts to clarify the interaction were inconclusive. After purification, microsequencing analysis identified p14 as Elongin C, p19 as Elongin B, and pp84 as VACM-1/Cul5. These proteins are known to exist in a complex containing E3-ligase activity, necessary for ubiquitin-proteasome mediated protein degradation. As E4orf6 acts with E1B-55kDa to induce p53 degradation, it may do so by recruiting this complex. Future studies will elucidate more about the role of E4orf6 in p53 degradation, and may reveal new functions of this viral protein.

APA, Harvard, Vancouver, ISO, and other styles

26

Goyer, Charles. "Characterization of yeast cap binding proteins." Thesis, McGill University, 1993. http://digitool.Library.McGill.CA:80/R/?func=dbin-jump-full&object_id=41144.

Full text

Abstract:

The prominent role played by the cap structure in ribosome binding is mediated by the cap binding protein complex (eIF-4F). The importance of eIF-4F in the regulation of gene expression has been demonstrated in both mammalian and yeast cells. Nevertheless, the function of the high molecular weight subunit of eIF-4F is unknown. Here we describe the isolation and characterization of yeast eIF-4F (24- and 150-kD) as well as a novel CBP of 96-kD. The yeast gene TIF4631 encoding p150 and a closely related gene, TIF4632 were isolated. TIF4631 and TIF4632 are 53% identical, carry out an essential function, display sequences closely resembling the RNA recognition motif (RRM) and are homologous to the high molecular weight subunit of human eIF-4F (p220). The presence of an RRM-like sequence in TIF4631 is consistent with its RNA binding properties and promises to challenge the current views on how cap-dependent and cap-independent ribosome binding operate in eukaryotes.

APA, Harvard, Vancouver, ISO, and other styles

27

Hill, Andrew Francis. "Molecular studies of human prion proteins." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299942.

Full text

APA, Harvard, Vancouver, ISO, and other styles

28

Rothwell, Dominic G. "Characterisation of human DNA repair proteins." Thesis, University of Oxford, 1997. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.364145.

Full text

APA, Harvard, Vancouver, ISO, and other styles

29

Downing, Anna Kristina. "NMR structural studies and modelling proteins." Thesis, University of Oxford, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.334225.

Full text

APA, Harvard, Vancouver, ISO, and other styles

30

Lester, Jill. "Biochemical and crystallographic studies of proteins." Thesis, Liverpool John Moores University, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.292313.

Full text

APA, Harvard, Vancouver, ISO, and other styles

31

Gibb, G. M. "Characterization and biosynthesis of mitochondrial proteins." Thesis, University of Glasgow, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.374517.

Full text

APA, Harvard, Vancouver, ISO, and other styles

32

Betts, Tyrone. "Molecular modelling of calcium modulated proteins." Thesis, University of Newcastle Upon Tyne, 1993. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.308880.

Full text

APA, Harvard, Vancouver, ISO, and other styles

33

Massiah, Andrea Juliet. "Studies on wheat ribosome-inactivating proteins." Thesis, University of Warwick, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.283492.

Full text

APA, Harvard, Vancouver, ISO, and other styles

34

Knott, Tracy Gail. "Targeting of thylakoidal proteins into chloroplasts." Thesis, University of Warwick, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.307338.

Full text

APA, Harvard, Vancouver, ISO, and other styles

35

Newman, Christopher M. H. "Posttranslational processing of GTP-binding proteins." Thesis, University College London (University of London), 1992. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314811.

Full text

APA, Harvard, Vancouver, ISO, and other styles

36

Williams, Sande G. "Probing Protein-protein Interactions Among Proteins of a Nonaggregated Fatty Acid Synthetase From Euglena Gracilis Variety Bacillaris." Digital Commons @ East Tennessee State University, 1993. https://dc.etsu.edu/etd/2830.

Full text

Abstract:

Enoyl-acyl carrier protein (ACP) reductase from chloroplast nonaggregated fatty acid synthetase (FAS) of Euglena gracilis variety bacillaris was purified to a single band on a denaturing polyacrylamide gel. The enzyme was partially characterized with respect to substrate specificity, reduced nucleotide requirement, and the effect of ACP and Ca$\sp{++}$ on enzyme activity. Antibodies against the purified protein were raised in hens and isolated from eggs. ACP was purified from Euglena in yields of about 1mg/100g (wet weight) of cells. Antibodies were raised against the purified protein. ACP antibodies inhibited the Euglena chloroplast FAS using Euglena or E. coli ACP as a substrate. Comparisons with other ACPs included the following items: biological activity, pI, behavior in size exclusion media, and amino acid sequence of the N-terminal portion of the molecule. ACPs from E. coli and Euglena have been shown to interact with melittin, a cationic peptide from bee venom. E. coli ACP is a small (Mr, 8847), acidic, Ca$\sp{++}$-binding protein which possesses some characteristics resembling those of regulatory Ca$\sp{++}$-binding proteins including interaction with melittin. Melittin inhibited activity of the nonaggregated FAS from Euglena using either E. coli or Euglena ACP as a substrate. The peptide also inhibited activity of the aggregated FAS from Euglena. Antibodies against melittin were raised. Anti-melittin inhibited activity of both the nonaggregated and aggregated FAS enzyme systems from Euglena relative to nonimmune antibody. Investigation of inhibition of the nonaggregated FAS enzyme system demonstrated that acetyl-CoA-ACP transacylase, malonyl-CoA-ACP transacylase, and keto-acyl-ACP synthetase activities were inhibited to different degrees by anti-melittin antibodies, while keto-acyl-ACP reductase and enoyl-ACP reductase enzyme activities were not inhibited.

APA, Harvard, Vancouver, ISO, and other styles

37

Nilsson, Mikael. "Protein-DNA recognition : in vitro evolution and characterization of DNA-binding proteins /." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4269.

Full text

APA, Harvard, Vancouver, ISO, and other styles

38

Garner, Thomas Peter. "Structural and biophysical investigations into ubiquitin binding proteins." Thesis, University of Nottingham, 2011. http://eprints.nottingham.ac.uk/12100/.

Full text

Abstract:

The complicated task of interpreting the many ubiquitin signals is mediated by specific ubiquitin binding domains. The investigations discussed in this thesis focus on two very different ubiquitin binding domains. Chapters 3 to 5 detail the structural characterisation of the ubiquitin binding protein ZNF216. The structure of the ubiquitin binding Znf_A20 domain has been determined using multidimensional NMR techniques. A thermodynamic and structural characterisation of the interaction between the Znf_A20 and ubiquitin has been performed utilising chemical shift mapping, PRE based approaches, ESI-MS and ITC. The Znf_A20 domain forms a high affinity complex with Ub utilising a non-canonical binding site on ubiquitin centred at Asp58. The investigation was extended to the function of the Znf_A20 domain in the context of the full length protein. ZNF216, like many other ubiquitin receptors, has a ‘hook and line’ domain architecture with two independent domain separated by a long disordered linker. Chapters 6, 7 and 8 focus on the UBA domain of p62. The p62-UBA domain has been identified as a ‘hot spot’ for mutations linked to Paget’s disease of bone, a bone disorder which affects >3% of the over 55s. The dimerisation of the p62-UBA domain has been shown here to be a novel regulatory mechanism for the ubiquitin binding properties of p62. Modulation of both dimerisation and ubiquitin recognition are potential mechanisms by which mutations may disrupt p62 function and this prospect has been investigated here. The final chapter of this thesis examines the possibility of cooperation between different ubiquitin binding domains by simultaneous interaction with Ub to form ternary complexes. Using the Znf_A20 and the p62-UBA as an example the formation of a ternary complex has been demonstrated. By examining the available ubiquitin complexes it has been suggested that the formation of Ub mediated ternary complexes is limited to only a few UBD pairings. The cormation of Ub mediated ternary complexes may have interesting implications for the formation of larger multi-protein complexes utilising ubiquitin as an interaction hub on the recognition of poly-Ub with chain linkage specificity; and for Ub mediated signalling in general.

APA, Harvard, Vancouver, ISO, and other styles

39

McGee, John Hanney. "Evolving a Direct Inhibitor of the Ras Proteins." Thesis, Harvard University, 2013. http://dissertations.umi.com/gsas.harvard:10915.

Full text

Abstract:

In recent years, great advances have been made in understanding the molecular causes of human disease, but our ability to exploit these discoveries for therapeutic benefit is frequently limited by the inability to make drugs that target the processes responsible. Many diseases can be linked to the aberrant activity of proteins, and while the development of inhibitors for enzymes and extracellular targets is often feasible, these proteins account for only a small fraction of all the proteins in cells. The remaining proteins are, in most cases, considered therapeutically intractable and are sometimes referred to as "undruggable." Many proteins, particularly in higher organisms, carry out their activity in part through interactions with other proteins and biomolecules. The ability to specifically disrupt these interactions could have great therapeutic benefit, as it may provide a means of targeting otherwise intractable processes. The focus of this dissertation is on the development and characterization of molecules that inhibit the interactions of an “undruggable” protein target, Ras, which is linked to both the initiation and progression of a wide array of human cancers. Our approach has been to use high-throughput screening, coupled with directed evolution, to identify and improve small proteins (peptides) that bind Ras and block its ability to engage the effector proteins necessary for its oncogenic activity. We report these efforts, along with a series of biochemical experiments aimed at characterizing the properties and binding mechanism of the peptides discovered in the screen. These peptides bind the three human Ras proteins with mid-to-low nanomolar affinity, and with high specificity for Ras proteins over their close family members. The peptides directly engage the Ras effector domain, and can block Ras from binding a canonical effector protein in the context of cancer cell lysates. Based on a series of observations, we hypothesize that the peptides bind Ras as head-to-tail homodimers, and report preliminary attempts to exploit this observation and identify peptides with improved affinity to Ras. Finally, we discuss the preliminary results from a conceptually related effort to identify peptide inhibitors of the Myc transcription factor, which is another protein heavily implicated in human cancer.

APA, Harvard, Vancouver, ISO, and other styles

40

Dizin, Eric Michel. "Insights On Iron-Sulfur Cluster Assembly Donor Proteins." The Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1208532379.

Full text

APA, Harvard, Vancouver, ISO, and other styles

41

Yu, Lu. "Structural Studies of Human Prion Proteins and Peptides." Case Western Reserve University School of Graduate Studies / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=case1447338491.

Full text

APA, Harvard, Vancouver, ISO, and other styles

42

Bonham, Victoria Anne. "Secondary cell wall specific proteins in plants." Thesis, Royal Holloway, University of London, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.312839.

Full text

APA, Harvard, Vancouver, ISO, and other styles

43

Wainwright, David Michael. "Growth factor presentation by CD44 variant proteins." Thesis, Imperial College London, 1998. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.299241.

Full text

APA, Harvard, Vancouver, ISO, and other styles

44

Berks, Benjamin Charles. "Molecular characterisation of bacterial electron transport proteins." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.302884.

Full text

APA, Harvard, Vancouver, ISO, and other styles

45

Ponting, Christopher Paul. "Structural studies of plasminogen and related proteins." Thesis, University of Oxford, 1991. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.386861.

Full text

APA, Harvard, Vancouver, ISO, and other styles

46

Whiteway, Clare Ann. "Structural and thermodynamic studies on retinal proteins." Thesis, University of Oxford, 1994. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.240487.

Full text

APA, Harvard, Vancouver, ISO, and other styles

47

Carey, Siobhan M. "Amyloid and associated proteins in Alzheimer's disease." Thesis, Queen's University Belfast, 1996. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.337647.

Full text

APA, Harvard, Vancouver, ISO, and other styles

48

Carter, J. M. "Monoclonal antibody probes of legume storage proteins." Thesis, University of East Anglia, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.384593.

Full text

APA, Harvard, Vancouver, ISO, and other styles

49

Davies, R. J. "Monolayer studies on intrinsic erythrocyte membrane proteins." Thesis, University of Manchester, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356110.

Full text

APA, Harvard, Vancouver, ISO, and other styles

50

Prigmore, Elena. "Rho family binding proteins in human neutrophils." Thesis, University College London (University of London), 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.314081.

Full text

APA, Harvard, Vancouver, ISO, and other styles

Dissertations / Theses on the topic 'Biochemistry of proteins'

Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles