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Relevant bibliographies by topics / Biochemistry – Research

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Academic literature on the topic 'Biochemistry – Research'

Author: Grafiati

Published: 4 June 2021

Last updated: 30 January 2023

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Journal articles on the topic "Biochemistry – Research"

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Fartushok, Tetiana V., Nadiia V. Fartushok, Yu M. Fedevych, and Vladyslav V. Pyndus. "HISTORY OF BIOCHEMISTRY IN LVIV." Wiadomości Lekarskie 75, no. 4 (2022): 881–90. http://dx.doi.org/10.36740/wlek202204124.

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The aim: The purpose of this literature review is to shed light on the development of biochemical knowledge in the Lviv region and on prominent figures in the development of biochemistry during the Second World War. Materials and methods: Review of literature published before 2020. We searched the literature using the search terms ‘biochemists’, ‘ Lviv National Medical University’, ‘second World War’. Conclusions: The development of biological research in Lviv can be divided into two historical stages: 1) from the beginning of the founding of Lviv University in 1661 to the First World War; 2) between the First and Second World Wars and after the Second World War. Biochemical research was initiated at the Medical Faculty of Lviv University. In 1939, the Lviv State Medical Institute was established on the basis of the Medical Faculty of the University, where a powerful department of biochemistry functioned, which was headed by a worldclass biochemist – Jakub Parnas.

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Majkic-Singh, Nada. "Society of medical biochemists of Serbia and Montenegro: 50 years anniversary." Jugoslovenska medicinska biohemija 24, no. 3 (2005): 157–70. http://dx.doi.org/10.2298/jmh0503157m.

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Medical biochemistry (synonyms: clinical chemistry or clinical biochemistry) in the terms of professional and scientific discipline, stems from and/or has developed along with the natural sciences and its influences (mathematics, physics, chemistry and biochemistry) and medical sciences as well (physiology, genetics, cell biology). As a scientific discipline, medical biochemistry studies metabolic processes of physiological and pathological changes with humans and animals. Applying analytical chemistry's and biochemistry's techniques enables medical biochemists to gain plenty of information related to diagnosis and prognosis which serve physicians to asses the gravity of illness and prescribe healing therapy. Therefore medical biochemistry is an integral part of modern medicine. This discipline was dubbed various, often confusing names such as pathology, physiology, clinical biology, clinical pathology, chemical pathology, clinical biochemistry, medical biochemistry, clinical chemistry and laboratory medicine, all depending on place of origin. The official, internationally accepted name - clinical chemistry, was mentioned for the first time in 1912 by Johan Scherer, who described his laboratory as Clinical Chemistry Laboratory (Klinisch Chemische Laboratorium) in the hospital Julius in Wurzburg in Germany. After creating national societies of clinical chemists, Professor Earl J. King of Royal Postgraduate Medical School from London incited an initiative to unite national societies into the organization with worldwide character - it was the International Association of Clinical Biochemists, monitored by the International Union for Pure and Applied Chemistry (IUPAC). On 24 July 1952 in Paris, a Second International Congress of Biochemistry was held. A year later, in Stockholm, the name of a newly formed association was altered into International Federation of Clinical Chemistry, which was officially accepted in 1955 in Brussels. Today this federation-s name is International Federation for Clinical Chemistry and Laboratory Medicine (IFCC). Right after the World War II our medical biochemists began to gather within their expert societies. Even before 1950 Pharmaceutical Society of Serbia hosted laboratory experts among whom the most active were Prof. Dr. Aleksandar Damanski for bromatology, Prof. Dr. Momcilo Mokranjac for toxicology and Docent Dr. Pavle Trpinac for biochemistry. When the Managing Board of the Pharmaceutical Society of National Republic of Serbia held its session on 22 December 1950, an issue was raised with reference to creation of a Section that would gather together the laboratory experts. Section for Sanitary Chemistry, combining all three profiles of laboratory staff, i.e. medical biochemists, sanitary chemists and toxicologists, was founded on 1st of January 1951. On 15 May 1955, during the sixth plenum of the Society of Pharmaceutical Societies of Yugoslavia (SFRY) held in Split, the decision was passed to set up a Section for Medical Biochemistry in SFDJ. The Section for Medical Biochemistry in SFDJ was renamed into Society for Medical Biochemistry of SFDJ based on the decision passed during the 16th plenum of SFDJ, held on 15 May 1965 in Banja Luka. Pursuant to the decision passed by SMBY on 6 April 1995 and based on the historic data, 15 May was declared as being the official Day of the Society of Medical Biochemists of Yugoslavia. The purpose of YuSMB (currently SMBSCG) is to gather medical biochemists who would develop and enhance all the branches of medical biochemistry in health industry. Its tasks are as following: to standardize operations in clinical-biochemical laboratories, education of young biochemists on all levels, encouraging scientific research, setting up of working norms and implementation, execution and abiding by the ethics codices with health workers. SMBSCG is to promote the systemized standards in the field of medical biochemistry with the relevant federal and republican institutions. SMBSCG is to enable exchange of experiences of its members with the members of affiliate associations in the country and abroad. .

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Saderholm, Matthew, and Anthony Reynolds. "Jmol-Enhanced Biochemistry Research Projects." Journal of Chemical Education 88, no. 8 (August 2011): 1074–78. http://dx.doi.org/10.1021/ed101022g.

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Slater, E. C. "Training for research in biochemistry." Biochemical Education 16, no. 3 (July 1988): 133–35. http://dx.doi.org/10.1016/0307-4412(88)90181-1.

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Mocz, Gabor. "A Changing Research and Publication Landscape for Biochemistry." Biochemistry Insights 1 (January 2008): 117862640800100. http://dx.doi.org/10.1177/117862640800100001.

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This introductory editorial hopes to convey three points to its audience. First, it provides an overview of the new, peer-reviewed, open access journal Biochemistry Insights published by Libertas Academica. Second, it summarizes the benefits of open access publishing concepts to the biochemistry community. And third, it takes a brief look at the near future of biochemistry as a fundamental molecular science whose continued advances and latest developments will be the focus of the new journal. Biochemistry Insights looks forward to receiving research articles, review papers, commentaries and letters from all disciplines and specialties of the field.

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Lang, Franziska K., and George M. Bodner. "A Review of Biochemistry Education Research." Journal of Chemical Education 97, no. 8 (June 29, 2020): 2091–103. http://dx.doi.org/10.1021/acs.jchemed.9b01175.

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Kanoksilapatham, Budsaba. "Rhetorical structure of biochemistry research articles." English for Specific Purposes 24, no. 3 (January 2005): 269–92. http://dx.doi.org/10.1016/j.esp.2004.08.003.

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Blundell, Tom. "Introduction." Biochemist 33, no. 5 (October 1, 2011): 4–5. http://dx.doi.org/10.1042/bio03305004.

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This issue of The Biochemist is focused on biochemistry in China. It is timely because it reflects the history of biochemical research collaboration between Chinese and UK scientists, not only by looking back over the last century, but also by reviewing some of the strengths of biochemical research in China in 2011.

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Milosevic Georgiev, Andrijana, Dušanka Krajnović, Jelena Manojlović, Svetlana Ignatović, and Nada Majkić Singh. "Seventy Years of Biochemical Subjects’ Development in Pharmacy Curricula: Experience from Serbia/ Sedamdeset godina razvoja biohemijskih predmeta u kurikulumu farmacije: iskustvo iz srbije." Journal of Medical Biochemistry 35, no. 1 (January 1, 2016): 69–79. http://dx.doi.org/10.1515/jomb-2015-0018.

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Summary Introduction: The pharmacists played an important role in the development of biochemistry as applied chemistry in Serbia. What is more, the first seven state chemists in Ser bia were pharmacists. State chemists performed the chemicaltoxicological analysis as well as some medical and biochemical ones. When it comes to the education of medical biochemists as health workers, the period after the beginning of the second half of the twentieth century should be taken into account because that is when the training of pharmaceutical staff of the Faculty of Pharmacy, University of Belgrade, begins on the territory of Serbia. This paper presents the development of medical biochemistry through the development of curriculum, personnel and literature since the foundation of the Faculty of Pharmacy in Serbia until today. Objective: The aim of this paper is to present the historical development of biochemistry at the Faculty of Pharmacy, University of Belgrade, through analysis of three indicators: undergraduate and postgraduate education of medical biochemists, teaching literature and professional associations and trade associations. Method: The method of direct data was applied in this paper. Also, desktop analysis was used for analyzing of secondary data, regulations, curricula, documents and bibliographic material. Desktop research was conducted and based on the following sources: Archives of the University of Belgrade- Faculty of Pharmacy, Museum of the History of Pharmacy at the University of Belgrade-Faculty of Pharmacy, the Society of Medical Biochemists of Serbia and the Serbian Chamber of Biochemists. Results and conclusion: The curricula, the Bologna process of improving education, the expansion of the range of subjects, the number of students, professional literature for teaching biochemistry, as well as professional associations and trade associations are presented through the results.

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Winzor, Donald J. "Six decades of research in physical biochemistry." Biophysical Reviews 8, no. 4 (October 28, 2016): 279–81. http://dx.doi.org/10.1007/s12551-016-0222-x.

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Dissertations / Theses on the topic "Biochemistry – Research"

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Pan, Xinlei. "Structure and mechanism of the exonuclease enzymes of viral SynExo recombination systems." The Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1429795341.

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Trivedi, Archit. "Bromodomain Containing Proteins in Melanocyte Differentiation and Melanoma." University of Toledo Health Science Campus / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=mco1438963150.

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ARAS, SHWETA. "Role of SWI/SNF Chromatin Remodeling Enzymes in Melanocyte Differentiation and DNA Damage Response to Ultraviolet Radiation." University of Toledo Health Science Campus / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=mco1438970443.

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Rodgers, Jennifer Leigh. "The Role of Hyaluronan Size in Murine Asthma." Cleveland State University / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=csu1464261240.

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Gupta, Yashi. "Understanding the Role of Macrophage Migration Inhibitory Factor (MIF) and its Homologue D-Dopachrome Tautomerase (DDT) in Cancer." Case Western Reserve University School of Graduate Studies / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=case1497113473427922.

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Othman, Rashidi. "Biochemistry and genetics of carotenoid composition in potato tubers." Diss., Lincoln University, 2009. http://hdl.handle.net/10182/1336.

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Potato cultivars exhibit a wide variation in skin and flesh colour due to the presence of pigments. This study established that potato cultivars differ greatly with respect to types and concentrations of carotenoids in tubers. A total of 46 cultivars were evaluated for quantitative and qualitative carotenoid composition in different growing seasons, locations, storage conditions and disease symptoms. Factors controlling carotenoid accumulation were also tested by developing an in vitro minituber system as a new high-throughput model system for carotenogenesis in potato tubers. Tuber flesh colour was found to correlate with total carotenoid content in potato cultivars grown in both New Zealand and Netherlands. The main carotenoids identified in 32 potato cultivars in New Zealand were lutein, neoxanthin, violaxanthin and β-carotene. The ratio of these carotenoids varies between cultivars. Neoxanthin was detected in only 13 cultivars (10.59 to 69.21µg/g DW); violaxanthin was found only in 1 cultivar (32.76 µg/g DW). Whereas lutein and β-carotene were found in most of the cultivars but the concentration varied from (0.00 to 160.63 µg/g DW) and (0.00 to 13.62 µg/g DW) respectively. The main carotenoids identified in 12 cultivars grown in the Netherlands were neoxanthin, violaxanthin and lutein, whereas zeaxanthin was not found in any of the cultivars analysed. Marked differences were observed between the same potato cultivars grown in New Zealand and the Netherlands. Therefore cultivars were analysed over a second growing season to assess stability in carotenoids composition. The carotenoid profiles of the potato tubers grown for two different seasons showed highly significant differences between the cultivars, the seasons, the carotenoid pigments, and all combinations of interactions, indicating the complex nature of factors influencing carotenoid composition. Reflectance colorimeter measurement of yellow hue component in this study confirmed that the higher the total carotenoid content, the greater the yellow intensity colour. Eight cultivars were grown at three locations in New Zealand and Agria and Desiree were grown at eight locations in the Netherlands to further investigate the stability of carotenoid composition. Highly significant differences were observed between the cultivars, the locations, the carotenoid pigments, and all combinations of interactions, which emphasises that changes in carotenoid composition are complex and the responses are not consistent across cultivars. Reflectance colorimeter measurement of yellow hue component confirmed the relationship between the yellow colour intensity of tuber flesh, as well as confirming the interaction between colour and locations. Disease and post harvest storage conditions markedly influenced the levels of total carotenoid, neoxanthin, violaxanthin, zeaxanthin, lutein and β-carotene in potatoes. The magnitude of these effects depends on the cultivar, time of storage, and the intensity of powdery scab symptoms. Results showed that long term storage resulted in the accumulation of neoxanthin, violaxanthin and zeaxanthin with a concomitant decreased of lutein, β-carotene and total carotenoid content. Genotypes infected with disease (lower and higher scab score) resulted in accumulation of violaxanthin, β-carotene and total carotenoid with a concomitant decreased in neoxanthin and lutein. A high-throughput model system for investigating carotenoid biogenesis in potato tubers was developed. This involved in vitro potato minitubers and was validated by assessing the effects of environmental variables, such as drought stress, light intensity and nutrient availability on carotenoid accumulation. Light influenced the presence of zeaxanthin, whereas water stress and nutrient strength influenced the accumulation of neoxanthin and violaxanthin. Although these factors had an effect on the carotenoid content and profile, the most influential factor appeared to be cultivar selection.

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REN, PEI. "Glutathione Reductase, Redox Homeostasis, and Mitochondrial Dysfunction." Kent State University / OhioLINK, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=kent1366643205.

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Kendig, Eric Lee. "Oxidative stress and inflammation as therapeutic targets of high-fat diet-induced metabolic diseases." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1242844513.

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Wang, Lin. "Synthesis and Characterization of Functionalized Recombinant Thrombomodulin for Antithrombotic Drug Development." Cleveland State University / OhioLINK, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=csu1441946591.

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Mbah, Nneka Elizabeth. "Defining the Mechanism of Methuosis, a Non-apoptotic Cell Death Pathway, Induced by Indolyl Chalcone Compounds in Glioblastoma Cells." University of Toledo Health Science Campus / OhioLINK, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=mco1481303173869378.

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Books on the topic "Biochemistry – Research"

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Walker, James T., and Catherine M. Jones. Biochemistry researcher biographical sketches and research summaries. Hauppauge, N.Y: Nova Science Publishers, 2011.

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Baginski, Simon J. Biochemistry research updates. Hauppauge, N.Y: Nova Science Publishers, 2011.

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G, Hughes Jason, and Robinson Alton J, eds. Inorganic biochemistry: Research progress. New York: Nova Science, 2008.

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1960-, Fuchs Stefan, and Auer Max 1962-, eds. Biochemistry and histocytochemistry research developments. Hauppauge, N.Y: Nova Science, 2009.

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Efremovich, Zaikov Gennadiĭ, and Lobo Victor M. M, eds. Biochemistry and chemistry: Research and development. New York: Nova Science Publishers, 2003.

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Varfolomeev, Sergeĭ Dmitrievich. Biochemistry and biotechnology: Research and development. Hauppauge, N.Y: Nova Science Publishers, 2011.

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name, No. International perspectives on chemistry and biochemistry research. Hauppauge, NY: Nova Science Publishers, 2003.

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Efremovich, Zaikov Gennadiĭ, Lobo Victor M. M, and Guarrotxena N, eds. International perspectives on chemistry and biochemistry research. New York: Nova Science, 2003.

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F, Lata Gene, ed. Experimental biochemistry. New York: Oxford University Press, 1989.

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Kornberg, Arthur. Biochemistry at Stanford, Biotechnology at DNAX. Berkeley, Calif: Regional Oral History Office, Bancroft Library, University of California, 1998.

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Book chapters on the topic "Biochemistry – Research"

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Ikeda, Tomoya, Naoto Tani, Tomomi Michiue, and Takaki Ishikawa. "Forensic Biochemistry." In Forensic Medicine and Human Cell Research, 13–27. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2297-6_2.

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Ginsburg, Hagai. "The biochemistry ofPlasmodium falciparum." In Advances in Malaria Research, 219–90. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2016. http://dx.doi.org/10.1002/9781118493816.ch9.

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Razin, Shmuel. "Mycoplasma Membranes as Models in Membrane Research." In Subcellular Biochemistry, 1–28. Boston, MA: Springer US, 1993. http://dx.doi.org/10.1007/978-1-4615-2924-8_1.

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Matthieu, J. M. "The Biochemistry of Myelin." In Multiple Sclerosis Research in Europe, 209–15. Dordrecht: Springer Netherlands, 1986. http://dx.doi.org/10.1007/978-94-009-4143-4_29.

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Evans, H. J., P. J. Bottomley, and W. E. Newton. "Biochemistry and Physiology of Mutants." In Nitrogen fixation research progress, 531–37. Dordrecht: Springer Netherlands, 1985. http://dx.doi.org/10.1007/978-94-009-5175-4_73.

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Naresh Kumar, S., K. B. Hebbar, K. V. Kasturi Bai, and V. Rajagopal. "Physiology and Biochemistry." In The Coconut Palm (Cocos nucifera L.) - Research and Development Perspectives, 443–88. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2754-4_9.

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Palma, Carlos, Jessica Jellins, Andrew Lai, Alexis Salas, America Campos, Shayna Sharma, Gregory Duncombe, Jon Hyett, and Carlos Salomon. "Extracellular Vesicles and Preeclampsia: Current Knowledge and Future Research Directions." In Subcellular Biochemistry, 455–82. Cham: Springer International Publishing, 2021. http://dx.doi.org/10.1007/978-3-030-67171-6_18.

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Conmy, Sarah, and Heinz-Peter Nasheuer. "The Use of Transgenic Mice in Cancer and Genome Stability Research." In Subcellular Biochemistry, 325–36. Dordrecht: Springer Netherlands, 2009. http://dx.doi.org/10.1007/978-90-481-3471-7_17.

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Barrett, John. "Physiology and Biochemistry of Echinostomes." In Echinostomes as Experimental Models for Biological Research, 199–212. Dordrecht: Springer Netherlands, 2000. http://dx.doi.org/10.1007/978-94-015-9606-0_10.

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Dey, Aparajita. "Cytochrome P450 2E1: Its Clinical Aspects and a Brief Perspective on the Current Research Scenario." In Subcellular Biochemistry, 1–104. Dordrecht: Springer Netherlands, 2013. http://dx.doi.org/10.1007/978-94-007-5881-0_1.

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Conference papers on the topic "Biochemistry – Research"

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"Standardizing Biochemistry Dataset for Medical Research." In International Conference on Health Informatics. SCITEPRESS - Science and and Technology Publications, 2014. http://dx.doi.org/10.5220/0004745802050210.

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Moraes Ventura, Ana Paula, Stephanie Araújo de Andrade, Cesar Emanoel Barbosa de Lima, José Moreira da Silva Neto, Josilene Aires Moreira, Patrícia Vasconcelos Leitão Moreira, Raissa Dália Paulino, and Ricardo Moreira da Silva. "GAMIFICATION IN FOOD BIOCHEMISTRY TEACHING AT UFPB BRAZIL IN PANDEMIC." In 14th annual International Conference of Education, Research and Innovation. IATED, 2021. http://dx.doi.org/10.21125/iceri.2021.0946.

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Jiang, Rui, Ziyan Zhang, Han Wu, and Chao Zhang. "Research Progress of Bone Tumor Clinical Diagnosis." In 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/bbbs-18.2018.21.

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Qi�e, Zhu. "Research on Strategy of Improving substantial Results of Sports Biochemistry Course." In 2014 Conference on Informatisation in Education, Management and Business (IEMB-14). Paris, France: Atlantis Press, 2014. http://dx.doi.org/10.2991/iemb-14.2014.78.

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Chavarriaga, Cristina, Ian McClure, Pablo Castello, Maria Procopio, Robert J. Usselman, and Carlos F. Martino. "The role of spin biochemistry in bioenergetics and reactive oxygen species product channeling." In 2016 Progress in Electromagnetic Research Symposium (PIERS). IEEE, 2016. http://dx.doi.org/10.1109/piers.2016.7735826.

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Hou, Yani, Min Shi, Hui Wang, Jia Liu, and Rongli Liu. "Application Research of Small Molecule Peptide Screening Based on Cancer Cells." In 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/bbbs-18.2018.15.

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Fei, Dan, Yang Li, and Guoqing Sui. "Research study on target treatment of ultrasound microbubbles with docetaxel on osteosarcoma." In 2018 International Workshop on Bioinformatics, Biochemistry, Biomedical Sciences (BBBS 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/bbbs-18.2018.5.

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Anwar, Yunita Arian Sani, Senam, and Endang W. Laksono. "Identification of the students’ critical thinking skills through biochemistry laboratory work report." In THE 4TH INTERNATIONAL CONFERENCE ON RESEARCH, IMPLEMENTATION, AND EDUCATION OF MATHEMATICS AND SCIENCE (4TH ICRIEMS): Research and Education for Developing Scientific Attitude in Sciences And Mathematics. Author(s), 2017. http://dx.doi.org/10.1063/1.4995112.

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Wang, Jianfei, Lian Jin, Weiqi Wang, and Xiaomei Wu. "A Mono-bidomain Electrophysiological Simulation Method for Electrical Defibrillation Research." In ICBBB '20: 2020 10th International Conference on Bioscience, Biochemistry and Bioinformatics. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3386052.3386074.

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Li, Ling, Yani Chang, Juanjuan Bai, and Tieling Liu. "The Thinking about the Biochemistry Teaching in Food Quality and Safety Specialty." In 2018 2nd International Conference on Education, Economics and Management Research (ICEEMR 2018). Paris, France: Atlantis Press, 2018. http://dx.doi.org/10.2991/iceemr-18.2018.136.

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Reports on the topic "Biochemistry – Research"

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Vakharia, Vikram, Shoshana Arad, Yonathan Zohar, Yacob Weinstein, Shamila Yusuff, and Arun Ammayappan. Development of Fish Edible Vaccines on the Yeast and Redmicroalgae Platforms. United States Department of Agriculture, February 2013. http://dx.doi.org/10.32747/2013.7699839.bard.

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Betanodaviruses are causative agents of viral nervous necrosis (VNN), a devastating disease of cultured marine fish worldwide. Betanodavirus (BTN) genome is composed of two single-stranded, positive-sense RNA molecules. The larger genomic segment, RNA1 (3.1 kb), encodes the RNA-dependent RNA polymerase, while the smaller genomic segment, RNA 2 (1.4kb), encodes the coat protein. This structural protein is the host-protective antigen of VNN which assembles to form virus-like particles (VLPs). BTNs are classified into four genotypes, designated red-spotted grouper nervous necrosis virus (RGNNV), barfin flounder nervous necrosis virus (BFNNV), tiger puffer nervous necrosis virus (TPNNV), and striped jack nervous necrosis virus (SJNNV), based on phylogenetic analysis of the coat protein sequences. RGNNV type is quite important as it has a broad host-range, infecting warm-water fish species. At present, there is no commercial vaccine available to prevent VNN in fish. The general goal of this research was to develop oral fish vaccines in yeast and red microalgae (Porphyridium sp.) against the RGNNV genotype. To achieve this, we planned to clone and sequence the coat protein gene of RGNNV, express the coat protein gene of RGNNV in yeast and red microalgae and evaluate the immune response in fish fed with recombinantVLPs antigens produced in yeast and algae. The collaboration between the Israeli group and the US group, having wide experience in red microalgae biochemistry, molecular genetics and large-scale cultivation, and the development of viral vaccines and eukaryotic protein expression systems, respectively, was synergistic to produce a vaccine for fish that would be cost-effective and efficacious against the betanodavirus infection.

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Weinberg, Zwi G., Richard E. Muck, Nathan Gollop, Gilad Ashbell, Paul J. Weimer, and Limin Kung, Jr. effect of lactic acid bacteria silage inoculants on the ruminal ecosystem, fiber digestibility and animal performance. United States Department of Agriculture, September 2003. http://dx.doi.org/10.32747/2003.7587222.bard.

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The overall objective of the whole research was to elucidate the mechanisms by which LAB silage inoculants enhance ruminant performance. The results generated will permit the development of better silage inoculants that maximize both silage preservation and animal performance. For this one-year BARD feasibility study, the objectives were to: 1. determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF) 2.select the inoculants that survived best, and 3. test whether LAB silage inoculants produce bacteriocins-like substances. The most promising strains will be used in the next steps of the research. Silage inoculants containing LAB are used in order to improve forage preservation efficiency. In addition, silage inoculants enhance animal performance in many cases. This includes improvements in feed intake, liveweight gain and milk production in 25-40% of studies reviewed. The cause for the improvement in animal performance is not clear but appears to be other than direct effect of LAB inoculants on silage fermentation. Results from various studies suggest a possible probiotic effect. Our hypothesis is that specific LAB strains interact with rumen microorganisms which results in enhanced rumen functionality and animal performance. The first step of the research is to determine whether LAB of silage inoculants survive in RF. Silage inoculants (12 in the U.S. and 10 in Israel) were added to clarified and strained RF. Inoculation rate was 10 ⁶ (clarified RF), 10⁷ (strained RF) (in the U.S.) and 10⁷, 10⁸ CFU ml⁻¹ in Israel (strained RF). The inoculated RF was incubated for 72 and 96 h at 39°C, with and without 5 g 1⁻¹ glucose. Changes in pH, LAB numbers and fermentation products were monitored throughout the incubation period. The results indicated that LAB silage inoculants can survive in RF. The inoculants with the highest counts after 72 h incubation in rumen fluid were Lactobacillus plantarum MTD1 and a L. plantarum/P. cerevisiae mixture (USA) and Enterococcus faecium strains and Lactobacillus buchneri (Israel). Incubation of rumen fluid with silage LAB inoculants resulted in higher pH values in most cases as compared with that of un-inoculated controls. The magnitude of the effect varied among inoculants and typically was enhanced with the inoculants that survived best. This might suggest the mode of action of LAB silage inoculants in the rumen as higher pH enhances fibrolytic microorganisms in the rumen. Volatile fatty acid (VFA) concentrations in the inoculated RF tended to be lower than in the control RF after incubation. However, L. plalltarull1 MTDI resulted in the highest concentrations of VFA in the RF relative to other inoculants. The implication of this result is not as yet clear. In previous research by others, feeding silages which were inoculated with this strain consistently enhanced animal performance. These finding were recently published in Weinberg et.al.. (2003), J. of Applied Microbiology 94:1066-1071 and in Weinberg et al.. (2003), Applied Biochemistry and Biotechnology (accepted). In addition, some strains in our studies have shown bacteriocins like activity. These included Pediococcus pentosaceus, Enterococcus faecium and Lactobacillus plantarum Mill 1. These results will enable us to continue the research with the LAB strains that survived best in the rumen fluid and have the highest potential to affect the rumen environment.

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