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Larsson, Andréas. "Biochip design based on tailored ethylene glycols /." Linköping : Division of Molecular Physics, Department of Physics, Chemistry and Biology, Linköping University, 2007. http://www.bibl.liu.se/liupubl/disp/disp2007/tek1111s.pdf.
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Shim, Ji Wook. "Single-molecule investigation and nanopore-integrated biochip." Diss., Columbia, Mo. : University of Missouri-Columbia, 2008. http://hdl.handle.net/10355/6673.
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Thesis (Ph. D.)--University of Missouri-Columbia, 2008.<br>The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from title screen of research.pdf file (viewed on June 19, 2009) Includes bibliographical references.
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Larsson, (Kaiser) Andréas. "Biochip design based on tailored ethylene glycols." Doctoral thesis, Linköpings universitet, Sensorvetenskap och Molekylfysik, 2007. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-9578.
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Studies of biomolecular interactions are of interest for several reasons. Beside basic research, the knowledge gained from such studies is also very valuable in for example drug target identification. Medical care is another area where biomolecules may be used as biomarkers to aid physicians in making correct diagnosis. In addition, the highly specific interactions between antibodies and almost any substance opens up the possibilities to design systems for detection of trace amounts of both biological and non-biological substances within environmental restoration, law enforcement, correctional care, customs service and national security. A biochip, which contains a biologically active material, offers a means of monitoring the molecular interactions in the above applications in a sensitive and specific manner. The biochip is a key component of a biosensor, which also includes components for transforming the interaction events into a human-readable signal. This thesis describes the use of poly(ethylene glycol) (PEG) in biochip design. Two different approaches are presented, the first based on ethylene glycol (EG)-containing alkyl thiol self-assembled monolayers (SAMs) on flat gold and the second on photo-induced graft copolymerisation of PEG-containing methacrylate monomers onto various substrates. The former is a two dimensional system where EG-terminated thiols are mixed with similar thiols presenting tail groups that mimic the explosive substance 2,4,6-trinitrotoluene (TNT). In an immunoassay, the detection limit for TNT was determined to fall in the range 1-10 µg/L. In the second approach, a branched three dimensional biosensor matrix (hydrogel) is proposed. The carboxymethylated (CM) dextran matrix, which is commonly used within the biosensing community, is not always ideal for studies of biointeractions, due to the non-specific binding frequently encountered in work with complex biological solutions and various proteins. To employ PEG, which displays a low non-specific binding of such species, is therefore an interesting option worth investigating. The use of a branched graft polymerised PEG matrix in biosensor applications is novel as compared to previous reports which have focused on linear PEG chains. The latter approach provides, at maximum, one functional group, per surface anchoring point, for immobilisation of sensor elements. Thus, it has the inherited disadvantage that it limits the number of available immobilisation sites. The present PEG matrix contains a large number of functional groups, for immobilisation of sensor elements, per grafting site and offers the potential of improved response upon binding to the analyte as demonstrated in a series of successful sensor experiments. Furthermore, the nature of the process enables easy preparation of matrix patterns and gradients. In a PEG matrix gradient, protein permeability is studied and the capabilities of immobilising proteins are demonstrated. By combining the patterning technique with different monomers in a two-step process, an inert platform, lacking chemical attachment sites, is provided with arrays of spots (with immobilisation capabilities), which are conveniently addressed via microdispensing and used for biosensor purposes. The EG-terminated thiols present another means of generating such inert platforms, a route which is also investigated. To further explore the sensor quality of these spots, the concepts of patterning and gradient formation are combined and studied.<br>Det är intressant att studera biomolekylära interaktioner av många anledningar. För att kunna bedriva framgångsrik läkemedelsutveckling är det oerhört viktigt att känna till hur olika molekyler samverkar i människokroppen. Inom sjukvården kan biomolekyler användas som biomarkörer, då närvaro av dem eller förändringar av deras koncentrationer är kopplade till sjukdomstillstånd, och därmed hjälper läkaren att ställa rätt diagnos. Dessutom kan de mycket specifika interaktionerna mellan antikroppar och (i princip) valfri substans användas för detektion av spårämnen vid miljösaneringsarbete, gränskontroller, polisarbete, fängelser och arbete med nationell säkerhet. Den här avhandlingen beskriver hur polymeren polyetylenglykol (PEG) kan användas vid design av biochip. Ett biochip är en liten anordning, som kan användas för att detektera specifika molekyler med hjälp av en biologisk interaktion. Traditionellt har PEG använts inom biomaterialsektorn, men återfinns även i hygienartiklar som tvål och tandkräm. Ett annat användningsområde är konservering av bärgade träskepp och i en del litiumjonbatterier ingår PEG som en komponent. Dessutom pågår utveckling av PEG-innehållande skyddsvästar. I det här arbetet används PEG framför allt på grund av sin förmåga att minimera ospecifik inbindning av proteiner, som utgör en stor del av gruppen biomolekyler, till ytor på biochip. Två olika typer av ytbeläggningar, som innehåller den här polymeren, har använts. Den första typen ger mycket tunna (~0.000003 mm), tvådimensionella filmer medan den andra ger en något tjockare (~0.00005 mm), tredimensionell struktur (matris). De tvådimensionella filmerna har använts för att utveckla en sprängämnesdetektor med mycket hög känslighet (detektionsgräns mellan 1-10 ppb). En viktig beståndsdel i detta system är antikroppar riktade mot sprängämnet trinitrotoluen (TNT). Den tredimensionella matrisen är mer generell och kan användas för att studera många olika molekylära interaktioner. Tillverkningsmetoden av matrisen är baserad på belysning med ultraviolett ljus och är därmed lämpad för att skapa mönstrade ytor. Genom att blockera delar av ljusflödet begränsas tillväxten av matrisen till de belysta delarna. På så sätt har bland annat så kallade mikro-arrayer, bestående av mikrometerstora (tusendels millimeter) strukturer i ett regelbundet mönster, tillverkats. Tekniken tillåter även tillverkning av gradienter, där matrisens tjocklek varierar längs med provet, genom att belysa olika delar av provytan olika länge. Genom att undersöka dessa gradienter har information om matrisens genomsläpplighet för proteiner kunnat extraheras. Gradientkonceptet har även kombinerats med mikro-arraytillverkningen och gett möjlighet att studera interaktioner mellan flera olika modellproteiner och deras motsvarande antikroppar i olika tjocka matriser på en och samma yta. Det finns ett stort antal sätt att utnyttja interaktionerna mellan olika molekyler på ett biochip. Ett tilltalande tillvägagångssätt är exempelvis att i en mikro-array binda in olika molekyler som kan fånga kliniskt intressanta biomolekyler, i syfte att skapa en hälsoprofil. Ett sådant biochip skulle ge möjlighet att parallellt detektera eller bestämma koncentrationen av ett stort antal biomolekyler i till exempel en droppe blod. På så sätt kan en diagnos snabbt ställas, kanske till och med utan att patienten behöver uppsöka sjukvården. Den utvecklade PEG-matrisen har god potential att fungera i en sådan applikation.
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Malachowski, Karl. "Mikrotechnische Realisierung und Charakterisierung einer Cuffelektrode mit hoher Ladungsübertragung für die Neurostimulation /." Templin : Detert, 2006. http://deposit.ddb.de/cgi-bin/dokserv?id=2766671&prov=M&dok_var=1&dok_ext=htm.
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Åsberg, Peter. "Hydrogels of conjugated polyelectrolytes for biosensor and biochip applications /." Linköping : Linköpings universitet, 2005. http://www.bibl.liu.se/liupubl/disp/disp2005/tek982s.pdf.
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O'Hagan, Paul. "Development of aptamer-based biochip assays for breast cancer." Thesis, Queen's University Belfast, 2006. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.437551.
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Candito, Antonio. "Modelling, simulation and characterization of epithelial cell culture biochip." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2015. http://amslaurea.unibo.it/8483/.
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A microfluidic Organ-on-Chip has been developed for monitoring the epithelial cells monolayer.
Equivalent circuit Model was used to determine the electrical properties from the impedance spectra of the epithelial cells monolayer.
Black platinum on platinum electrodes was electrochemically deposited onto the surface of electrodes to reduce the influence of the electrical double layer on the impedance measurements.
Measurements of impedance with an Impedance Analyzer were done to validate the equivalent circuit model and the decrease of the double layer effect.
A Lock-in Amplifier was designed to measure the impedance.
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Choi, Jeong-Woo. "Management strategy for open innovation in Korean biochip industry." Thesis, Durham University, 2017. http://etheses.dur.ac.uk/12145/.
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Due to the need to access external knowledge for new product development (NPD), open innovation has been widely used in the biochip industry. Since current resources owned by single firm do not have all the capabilities, NPD in biochip requires strong interdisciplinarity, wide diversity of technological knowledge, and integration capabilities. In the present study, management strategy for open innovation is investigated for NPD in the Korean biochip industry. Open innovation is classified in three steps: (1) switching phase about starting open innovation in the NPD initial stage; (2) implementation phase about open innovation management in the NPD middle stage; and (3) transition phase about change to close innovation in the NPD final stage. Three models for three phases are developed and then tested by carrying out surveys in the Korean biochip firms. In addition, the transition phase model is evaluated in the Korean bio-pharmaceutical firms. The switching phase model suggests that research capability and external trust are the main variables that affect switching cost, which relates to the perception of advantage of open innovation. The implementation phase model suggests that technological novelty affects degree of openness, which, in turn, relates to technological capability and firm performance. Furthermore, institutional-, environmental-, and firm characteristics affect the depth- and breadth of open innovation activity. The transition phase model suggests that knowledge connected with product innovation has an impact on the open-close transition tendency, which relates to perception of advantage of close innovation. Based on the results for three phases of open innovation, we propose the management strategy for open innovation during NPD. Therefore, based on the results of analyses of the proposed model, we can evaluate the factors that affect open innovation activity and develop an appropriate management strategy of open innovation for NPD of biochip.
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LAKI, ANDRAS JOZSEF. "Biochip-Integrable Microfluidic Particle Separation Techniques for Biomedical Use." Doctoral thesis, Politecnico di Torino, 2015. http://hdl.handle.net/11583/2585574.
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Biochip-integrable sorting and separation of micron-sized particles have an increasing importance in biomedical diagnostics, biochemical analyses, food and chemical processing, and environmental assessment. By employing the unique characteristics of microscale flow phenomena, various techniques have been established for fast and accurate separation, and to sort cells or particles in a continuous manner. As in classical separation procedures, the biochip-integrable size-fractionation of particles or cells could be realized by passive or active way. Passive procedures, which do not require external force-field, utilize the interaction between particles-particle, flow-particle, and the channel structure-particle to separate different-sized particles. Meanwhile, the active separation techniques make use of external force-field in various forms.
This doctoral thesis provides a novel biochip-integrable pathogen detection device (Flow Through Nematode Filter, FTNF), and a novel application of an asymmetric column structure, which called deterministic lateral displacement (DLD) device. The working principles are explained in detail, and performances of the devices are discussed with the results of the measurements.
The main target of this represented work is applications in medicine and biomedical research but we are also open for other application areas. The use of these simple microfluidic devices will make it possible to extend the use of cell-sorting to the point of care, closer to the patient at the clinic or in the field.
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Ljungblad, Jonas. "Antibody-conjugated Gold Nanoparticles integrated in a fluorescence based Biochip." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-50619.
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<p>Gold nanoparticles exhibit remarkable optical properties and could prove useful in sensitive biosensing applications. Upon illumination gold nanoparticles produce localized surface plasmons, which influence nearby fluorophores and an enhancement in their fluorescence intensity can be observed. This property makes gold nanoparticles attractive for enhancing optical signals.</p><p>In this project gold nanoparticles were functionalized with an antibody and immobilized to the surface of an existing biochip platform based on fluorescence. The aim was to investigate the possibility of obtaining an increased fluorescence signal from the gold nanoparticles. Two different conjugation procedures were investigated, direct physisorption and covalent attachment of the antibodies to the particles. Activity of bound antibodies was confirmed in both cases.</p><p>The on-chip fluorescence intensity produced by the different conjugates was monitored by use a specialized fluorescence reader designed for point-of-care use. AFM and SEM were used to determine the surface concentration of particles. A correlation between the produced fluorescence intensity and the surface concentration could be seen.</p>
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Gupta, Madhuri N. "Multi-Board Digital Microfluidic Biochip Synthesis with Droplet Crossover Optimization." University of Cincinnati / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1393237106.
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Ericsson, Emma. "Biosensor surface chemistry for oriented protein immobilization and biochip patterning." Licentiate thesis, Linköpings universitet, Sensorvetenskap och Molekylfysik, 2013. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-88102.
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This licentiate thesis is focused on two methods for protein immobilization to biosensor surfaces for future applications in protein microarray formats. The common denominator is a surface chemistry based on a gold substrate with a self-assembled monolayer (SAM) of functionalized alkanethiolates. Both methods involve photochemistry, in the first case for direct immobilization of proteins to the surface, in the other for grafting a hydrogel, which is then used for protein immobilization. Paper I describes the development and characterization of Chelation Assisted Photoimmobilization (CAP), a three-component surface chemistry that allows for covalent attachment and controlled orientation of the immobilized recognition molecule (ligand) and thereby provides a robust sensor surface for detection of analyte in solution. The concept was demonstrated using His-tagged IgG-Fc as the ligand and protein A as the analyte. Surprisingly, as concluded from IR spectroscopy and surface plasmon resonance (SPR) analysis, the binding ability of this bivalent ligand was found to be more than two times higher with random orientation obtained by amine coupling than with homogeneous orientation obtained by CAP. It is suggested that a multivalent ligand is less sensitive to orientation effects than a monovalent ligand and that island formation of the alkanethiolates used for CAP results in a locally high ligand density and steric hindrance. Paper II describes the development of nanoscale hydrogel structures. These were photografted on a SAM pattern obtained by dip-pen nanolithography (DPN) and subsequent backfilling. The hydrogel grew fast on the hydrophilic patterns and slower on the hydrophobic background, which contained a buried oligo(ethylene glycol) (OEG) chain. Using IR spectroscopy, it was found that the OEG part was degraded during UV light irradiation and acted as a sacrificial layer. In this process other OEG residues were exposed and acted as new starting points for the self-initiated photografting and photopolymerization (SIPGP). A biotin derivative was immobilized to the hydrogel density pattern and interaction with streptavidin was demonstrated by epifluorescence microscopy.
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Joseph, Rissen Alfonso. "A General Purpose Field-Programmable Digital Microfluidic Biochip with Scannable Electrofluidic Control." University of Cincinnati / OhioLINK, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1394725573.
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Kemmler, Manuel [Verfasser], and Albrecht [Akademischer Betreuer] Brandenburg. "Entwicklung eines präzisen, schnellen und automatischen Biochip-Systems für patientennahe Sepsis-Diagnostik." Freiburg : Universität, 2011. http://d-nb.info/1123460477/34.
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Chen, Chi-Tien, and 陳頎典. "Biochip Industry Analysis." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/42352722746032787127.
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碩士<br>臺灣大學<br>國際企業學研究所<br>98<br>People desire to find out the secret of the gene for hundreds of years. Until these decades, scientists finally can make their dream come true to unlock the code of gene and know the meanings of the arrangement. Therefore, the gene engineering has become a popular subject and developed fast and prosperously. During these decades, there is a new product catching many producers’ attention – Biochip.
This research analyzes the global biochip industry including the description of the biochip market structure and the business landscape, and predicts the expectation of the market in the future. This research concludes that both “costs” and “technology” are critical to the development of the biochip market. On the other hand, how to maintain the leading of the costs and technology is the key successful factor in this market. In addition, government is a very important role in this Industry. Without the approval of using biochip of government, the development of biochip in Taiwan will be hard to progress. Many important patents have been controlled by U.S and European international companies, and it limits the development of Taiwan firms. In this research, I try to find some directions of development out to give Taiwan firms some advices. The research tries to use the S-C-P theory and strategic group to improve the questions that Taiwan firms face.
Finally, this research suggests that (1) Taiwan firms should focus on the domain such as China, because Taiwan firms know more what customers need in Asia than foreign companies. (2) Many international companies put their resource on developing medical testing or research testing products.
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Su, chih-wen, and 蘇志溫. "Photoelectric response Bacteriorhodopsin biochip." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/21614661700251363176.
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Yang, Sheng-Chi, and 楊勝琦. "Biochip-microarray image analysis." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/89489994844625771946.
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碩士<br>國立中央大學<br>資訊管理研究所<br>92<br>Biochip – microarray is a new tool used to examine expression levels for thousands of genes simultaneously. In the process of the microarray experiment analysis, image acquisition and analysis is the first step and it is the important process. To analyze a high density microarray image with thousands of gene spots, how to segment the gene spot accurately from irregular shapes and crooked position directly affects the final result of bio-experiment. In this paper, spots the microarray image is focused on how to use an ideal edge detection algorithm – Canny edge detection algorithm - to segment accurately. By testing the microarray images obtained from the Standford Microarray Database. With the system(gridding、spot edge detection、dynamic gridding) built by in paper. The system is verified and proved to be able to provide a good method for each spot segmentation from an in input microarray accurately to raise the data precision.
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CHEN, SHIH-CHEN, and 陳仕振. "Patent and Infringement of Biochip." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/42964246514500000207.
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Chen, Yi-Lin, and 陳怡伶. "Analysis of the Biochip Industry." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/xedd88.
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碩士<br>國立交通大學<br>管理學院碩士在職專班科技管理組<br>98<br>In this study, interpretation according to different dimensions of the three biochip U.S. companies, analyze their key success factors, in order to provide domestic manufacturers in the future development trend, the emergence of new strategic planning direction.
The biochip market has excellent potential for new products, such as glycan chip, miRNA array, aCGH array, etc.. The global biochip industry, estimated future sales is still the highest in microarray, but the highest growth rate is Lab-on-a-chip.
The top three were biochip manufacturing company Affymetrix, Agilent and Illumina, and introduced the two domestic manufacturers of biological chips: PhalanxBiotechGroup, and Dr. Chip Biotech. According to the technology, financial reporting and analysis of the patents, found that Illumina is the next most potential companies.Biochip industry is located in the current market growth curve in the initial stage, through the five forces analysis, that "the bargaining power of suppliers is much larger than the chip maker", "buyer for the sellers have more control power", "potential entrants not easy to enter", " biochip is not yet the emergence of alternatives", and" there is special competing and cooperative relationship among firms". The biochip by analyzing the three major U.S. manufacturers, summary of the key success factors: "the leading technology", "flexible business strategy", "clear market positioning", "the expansion of marketing channels", and "strategic alliance". SWOT analysis of biochip industry in Taiwan can be seen nowadays, although there are some threats and weaknesses, but there are many strengths and opportunities.
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PAN, CHIH-LI, and 潘志理. "Silicon Biochip For Micro Electrophoresis." Thesis, 2001. http://ndltd.ncl.edu.tw/handle/60956008228407434636.
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碩士<br>國立臺灣大學<br>電機工程學研究所<br>89<br>The object of current study is to make use of the process of silicon semiconductor and the technique of MEMS to realize the DNA chip for micro electrophoresis. By the microchannel on silicon, we just need a small amount of reagent to be measured. We refer electrochemical methods and join our ideas to bring up some mathematic models to explain the behavior of these reagents in different conditions. With these results, we can distinguish some charged ions with different molecular weight. Therefore, it’s able to apply the method on DNA, and to distinguish different DNA by the chip.
This study has developed some simple different Si etchant for deep etching. And successfully, the Si etchant can make the microchannel be done. Furthermore, the current and voltage of these reagents can be measured precisely by high resolution measurement system. Combining with the electrical circuit method and the concept of physics and chemistry can build up mathematic model. Then,we simulate the model by computer programs.
After simulation by computer programs and the repeated experiments, it is feasible to distinguish different DNA molecule by the electrophoresis chip. It should be beneficial for the advance of biomedical diagnostic technology.
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Chen, Gu Ren, and 陳顧仁. "Probe Placement Problems on Biochip." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/31562608949604921321.
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碩士<br>中華大學<br>資訊工程學系碩士班<br>95<br>There are two kinds of spotted and photolithographic methods for biochip
manufacture process; the mask sequence of photolithographic includes three types:
non-periodic, periodically synchronous, and periodically asynchronous cases. The
thesis mainly focuses on probe placement problems with periodically synchronous
mask sequence of photolithographic.
Until now, related researches have proposed TSP(Traveling Salesmen Problem)
and row-epitaxial placement algorithms. In the thesis, we first make use of genetic
algorithms, and then derive random row-exchange algorithms, finally propose genetic
random row-exchange algorithms.
According to experimental results, genetic random row-exchange algorithms is
better than the past researches of measuring BMP(Border Minimization Problem).
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Liu, Chun liang, and 劉俊良. "Development of pneumatic pumping biochip." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/4wyw85.
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碩士<br>長庚大學<br>醫療機電工程研究所<br>104<br>Biochip is defined as a miniaturization of conventional equipment fabricated by using micro-electro-mechanical systems (MEMS) technology. The advantages include reduce of reagent consumption, decrease of the diagnostic duration, and simplification of operation procedures. By the mature development of biomedical MEMS technology, a number of complicated biochips have been fabricated. However, only a few clinical applications were demonstrated using biochips. In this work, micro-pneumatic pump actuated by membrane was developed and integrated into a biochip. Based on this development, 2 clinical applications were demonstrated: (1) Automated immunoassay biochip for the detection of influenza viruses; (2) Study of gene expression of periosteal cells under mechanical stimulation. Conventional immunoassay involves complicated processes. In order to develop portable miniaturized diagnostic devices, four themo-pneumatic actuators and a reactor chamber were integrated into a biochip. All reagents were pre-installed in the chamber above the actuators. User only applied 20 μl virus sample to the reactor chamber. Reagents could be manipulated sequentially and reacted with the sample. Immunoassay results represented by colorimetric signal were shown to the detection of H1N1 and H3N2 viruses.
Alternatively, in order to study the correlation between mechanical stimulation and gene expression of periosteal cells, a biochip providing 2-dimensional tensile stress was developed based on the deformation of membrane. Results revealed higher gene expression was found in isotopic stress than in anisotopic stress. The device was shown to be feasible for the investigation of regenerative medicine.
Keywords: Membrane, Microfluidics, Influenza, Tensile stress.
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Yu, An-dih, and 余安棣. "Preparation of Unidirectional Bacteriorhodopsin Photoelectric Biochip." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/94958569081953363984.
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碩士<br>國立臺灣科技大學<br>化學工程系<br>97<br>The cytoplasmic (CP) and extracellular (EC) sides of purple membrane (PM) from Halobacterium salinarium were each selectively conjugated with biotin for directional immobilization on ITO glass via biotin-streptavidin affinity interactions. The photocurrents of the PM-coated ITO chips, which were produced due to a proton gradient across PM generated by illuminated bacteriorhodopsin, a light-driven proton pump, were studied to investigate the optimal fabrication processes, evaluate the performances of those two differently oriented PM chips, and develop novel photocell systems. The results showed that the best photocurrent responses were achieved when 1.5 mg/mL and 3 mg/mL biotin-conjugated PM were used to coat streptavidin-coated ITO glass for 10 h to prepare the chips with the EC-side and CP-side facing ITO, respectively. In addition, reversion of the photocurrent polarity was observed at pH 5.75 using the chips with the PM EC-side facing ITO. Multilayer fabrication of unidirectionally oriented PM chips was successfully achieved using the developed side-selective biotin conjugation of PM according to the linear frequency decrease responses of layer-by-layer coated QCM chips. Finally, the fabrication of PM on ITO was confirmed by examining the morphology changes of each differently fabricated ITO glass. This study will benefit the future development of PM-film based photosensors, photoswitches, or photodiodes.
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Cheng, Shu-Ying, and 鄭淑櫻. "Innovation Device Research for Biochip Application." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/16907172063982634933.
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博士<br>國立中興大學<br>電機工程學系所<br>103<br>Innovation device research for biochip application is our focus in this study. It concludes two parts: First, for a germanium on insulator (GOI) structure to detect charged proteins and simulation research. Second, study the impact of silicon-germanium (SiGe) material on hole mobility. With a GOI structure, we study the sensitivity to specific charge distributions in the electrolyte solution that arise from protein binding to the semiconductor surface. The quantum mechanical charge density in the semiconductor has been fully taken to account. The potential change at the binding sites as a function of the protein charge and ionic strength is determined. Varies amount of aspartic acids, charged proteins are used in this study.
In the research of uniaxial stress effect and hole mobility of strained SiGe PMOSFETs, the hole mobility in a high Ge content (110) SiGe inversion layer is measured and simulated by a split capacitance–voltage method and a quantized method, respectively. We also explore the impact of external mechanical uniaxial stress on the SiGe (110) p-channel metal oxide semiconductor field effect transistor (PMOSFET). We obtained the corresponding piezoresistance coefficients of the SiGe (110) PMOSFET with external mechanical uniaxial stress parallel and perpendicular to the channel direction. Our study shows the effectiveness in combining external mechanical uniaxial stress and intrinsic biaxial compressive strain for the SiGe (110) PMOSFET. In the study of impact of strain on hole mobility in the inversion layer of PMOSFET with SiGe alloy thin film, advanced SiGe MOSFET devices are simple and low cost to manufacture. This work focuses on hole mobility in the inversion layer of PMOSFETs using alloy channel materials. The primary topic of this work is the theoretical calculation of effective mass and hole mobility in the SiGe PMOSFET inversion layer. The strain conditions considered in the calculations are intrinsic strain resulting from growing the SiGe alloy thin films on the three orientation Si substrates. The hole mobility of SiGe alloy inversion layer for PMOSFET under substrate strain and various germanium mole fractions are all investigated. The impact of wafer orientation and channel direction on the hole mobility is analyzed using the Kubo–Green-wood carrier mobility formalism.
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Hsiao, Yi-Hsing, and 蕭怡馨. "Development of automated microfluidic biochip platforms." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/um69mz.
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Jheng-JhangLi and 李政璋. "Implementation of PDMS Biochip by MEMS Technology." Thesis, 2010. http://ndltd.ncl.edu.tw/handle/64517756155604511895.
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碩士<br>國立成功大學<br>奈米科技暨微系統工程研究所<br>98<br>In recent years, many scholars in electrophysiological field have devoted to investigate the relationship among genes, proteins and diseases by patch-clamp technology. The investigation of ion channels in the level of gene expression and protein structure can illuminate pathogenesis of many diseases, and as such can discover potential therapeutic targets and contribute to new drug invention.
However, the drawbacks of conventional patch-clamp method are time-consuming and low through-put. On the other hand, it needs highly-skilled operation to clamp the cell membrane of single cell for achieving the giga-ohm seal resistance. For these reasons, the biochip platform with a simple procedure will not only replace the traditional patch clamp technology but also provide high through-put function. For the above reasons and the need of future electrophysiological studies, how to design a practical biochip platform is a crucial issue.
Therefore, we try to develop a low-cost and high-throughput biochip platform by polymer (PDMS and polystyrene foam) in this study. First of all, we employed two methods to fabricate the biochip platform. One is the traditional MEMS technology. By pattering and etching processes, we created the two and three levels pillar-shaped silicon masters, respectively. The other is Probe insertion. In this novel approach, the 1μm probe was inserted into polymer substrate to create the 1μm opening for clamping the cell membrane. Thus, we can fabricate the several apertures in the same time and achieve the massive produce easily.
Although the MEMS fabrication processes for manufacturing biochip platforms is time-consuming. it can control the pore size more precisely than Probe insertion. Nevertheless, the Probe insertion has advantages of low-cost, disposable, simple fabrication processes, and rapid massive product. Finally, the open resistance of PDMS chip and a Polystyrene foam manufactured by Probe insertion are 1MΩ and 4.7MΩ, respectively. On the other hand, the PDMS and Polystyrene foam have a lower dielectric constant than silicon-based substrate and have no parasitic capacitance on impedance measurement. Thus, the Polymer-based patch clamp chip manufactured by Probe insertion will be promising.
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TSAI, YIN-JEAN, and 蔡茵媜. "Development of Simple and Rapid Enrichment Biochip." Thesis, 2019. http://ndltd.ncl.edu.tw/handle/5ap2f4.
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碩士<br>中臺科技大學<br>生物科技暨醫學工程研究所<br>107<br>The biochip developments provide a variety of possibilities for biomedical investigations. In recent years, research on microfluidic biochips has focused on the separation of rare cells in circulating blood. Herein, the rare cells are important for providing targeted drugs for clinical disease detection or personalized treatment, which include the circulating tumor cells and nuclear red blood cells (NRBCs). They are usable to the targeted therapy and non-invasive detections for reducing the risk of invasive treatments. The conventional manufacturing method using MEMs has been a popular technique. However, it may cause a technique threshold for the non-engineering field researchers due to complicated procedures. This study proposes a new approach for implementing the point-of-care therapy with the easy-manufacture microfluidic enrichment biochip using the comment materials and instruments. The advantages of our microfluidic chip include easy and fast manufacturing, simple operation, and fast to capture the rare cells.
The microfluidic chip is composed of double-sided tapes and glass slides which are easily accessible materials. The double-sided tape is used to stick two glass slides to form a microchannel with cross section of 18×1×0.5 mm3. The capillary action is applied generate the flow power in the microchannel. The micro-pillars arrays were fabricated in the microchannel as cell captures by an advanced DLP 3D printer. The cells capture rate was demonstrated with particles (10 um) and rooster blood cells. In result, the capture rate of the capture array is estimated to 96 %. This study is expected to provide a possibility of simple, fast operation and manufacturing technique for fabricating enrichment biochip.
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Cheng, Yi-Szu, and 鄭伊斯. "Development of Image Analysis System for Biochip." Thesis, 2012. http://ndltd.ncl.edu.tw/handle/86946977182680729259.
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碩士<br>中原大學<br>生物醫學工程研究所<br>100<br>The purpose of this study is to develop an image analysis system for 5×5 and/or 6×6 microarray biochip which is can reduce time cost of manual interpretation image and improve the efficiency of analysis.
Several image processing methods were used to develop system. First, this system changed biochip image from color image into grayscale image. The multi well biochip image was segmented to a single well by Radon transform. Then, the Sobel masker was used to enhance the image edge and then the image was bi-leveled. In addition, using circle equation, reaction point was gotten from bi-leveled image. Final, reaction point which captured from system was compared with pre-set characteristic pattern and analyzed its results. Moreover, in this system, feature type of chip could change by each experimental setting feature spot, and establish the sample database to compare with captured biochip image. The phantom images were constructed for testing the limitation of this system. And 50 pieces of 5×5 and 30 pieces of 6×6 microarray image were used for our system verification.
Results show that system minimum detection range for the gray value difference between the reaction point and background is 25, and the size of pixels is diameter greater than 9. In real data, the system accuracy was 90%, in which 5×5 microarray images were 92% and 6×6 microarray images were 87%. Furthermore, the sensitivity of reaction point in microarray images was 98.36%, specificity was 100%, accuracy was 99.61% and Kappa value was 98.92.
In order to improve this system, two analysis stages is proposed in the future. First stage, images will divide into clear and unclear type. Through the automatic interpretation, the clear images will improve accuracy up to 100%. The second stage, the other image processing methods will be used for unclear images or image information will be provided for user as a base of manual interpretation.
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Tsai, Han-Sheng, and 蔡瀚陞. "Cell Separation Biochip via Dielectrophoresis and Microfluidic Focusing." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/44598221713580645469.
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碩士<br>國立清華大學<br>奈米工程與微系統研究所<br>96<br>Recently, researches about manipulation and analysis of bio-particles by MEMS technology are growing because of the fabrication development, and it is an important field ob bio-technology. The main purpose is to integrate the functions we need into a Lab-On-a-Chip system, and the chips have the function of bio-detection and analysis. Taking the micro-device of cell sorting for example, there are many technology have been presented, such as microgripper. But each of them has some restrications. Therefore, we want to design a bio-chip which could separate cells easily and efficiently.
In this thesis, we use the characteristic of microfluid and design the bio-chip to combine with DEP (dielectrophoresis) and microchannel to sort cells. By collecting the two fluids with different flow rates, all cells will close the wall in the microchannel. At the same time, the cells will separate preliminary because of the sizes of the cells and the drag force. By applying input AC voltage within specific frequency range on the micro electrodes, cells could be separate and collect with negative DEP force. We through numerical simulation software, CFDRC, and the design concepts are realized by MEME fabrication process. At last, we use polystyrene beads in place of cells to demonstrate the functions of our chip.
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Chen, Ying Tng, and 陳盈廷. "Development and Detection of Micro Electrophoresis PDMS Biochip." Thesis, 2004. http://ndltd.ncl.edu.tw/handle/33646400467394693269.
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Liu, Nan-Ching, and 劉南青. "The Application of Dielectrophoresis Biochip on Cell Separation." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/97235825906560449075.
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碩士<br>大同大學<br>機械工程學系(所)<br>94<br>The most importance of separating small cells is the accuracy and efficiency, the more important consideration is that cells won’t suffer destroy from the separating process. Thus, we designed the dielectrophoresis separation chip according to the consideration. The designed electrode will cause non-uniform electric field, polarized cells would move to the place where the electric field magnitude is bigger or smaller, by the characteristic cells can separated. The dielectric properties of suspending medium and cells are relating to conductivity, dielectric constant and applied frequency. In experiment we changed medium conductivity and applied frequency to observe the dielectrophoretic phenomenon of live and dead yeast cells. We used PDMS as channel, it is easily to observe dielectrophoretic effect and bond with electrode chip.
We mainly use CFDRC software to find out the place of bigger or smaller electric field magnitude, let us know the place where positive and negative dielectrophoretic force occurred.
We separate live and dead cells successfully with two ways mention in the thesis. We discuss the dielectrophoretic force distribution and separation effect with different electrode shape.
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Yu, Wen-cheng, and 余文程. "The Application of Dielectrophoresis Biochip on Cell Lysis." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/46552261047177433610.
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碩士<br>大同大學<br>機械工程學系(所)<br>94<br>This thesis studies cell lysis and dielectrophoresis cell manipulation with biochip. Compared to conventional technology, the MEMS based biochip technique has the advantages like the better efficiency, lower assay consumption, less power and easy manipulation. For cell lysis, the biochip was fabrication by using Corning 1737 as the substrate and the electrode was deposited by evaporation and patterned by lithography. The microchannels were made on PDMS by soft lithography. Two cells (yeast cell and human white cell) were used to for cell lysis. The minimum cell lysis operation condition is investigated by numerical analysis with commercial software Femlab. The simulation results agree with the experimental results very well. Different operation parameters like electrode size, applied voltage were applied to study the efficiency. The results show that when the size of the electrode increases, the efficiency of cell lysis increases. On the other hand, the cell lysis efficiency is very sensitive to the magnitude of the applied voltage and the length of the impulse. For lower applied voltage, the longer impulse will cause cell lysis while higher applied voltage, the impulse duration can be shorter. For electrodeless dielectrophoresis biochip, the yeast cell moves along the direction of the electric field. The speed of the cell movement increases with increasing applied voltage. The nature of the cell movement needs further investigation.
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Tsai, Edward Yee-Hua, and 蔡宜樺. "Single Nucleotide Polymorphism Detection with Nano-Electorde Biochip." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/30829240244447065298.
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碩士<br>國立臺灣大學<br>機械工程學研究所<br>91<br>In this study, an electrical DNA detection method is reported by the peculiar conductivity and bio-affinity of gold nanoparticles. The nucleic acid was used as a interconnect to immobilize the gold nanoparticles onto the nano-electrode gap and that leaded to the conductivity changes. The gap of nano-electrode has been manufactured less than 200 nm by E-beam lithograghy technology in the early study. The self-assembly of Au nanoparticles layers linked onto the oligonucleotide- functionalized substrate were formed via the complementary DNA hybridization, which resulted in an increased surface densities of gold nanoparticles. The electrical method can detect the target DNA concentration at lowest 10 pM without a silver enhance step, which is required in the other methodologies.
For the application of this mechanism to SNP analyst, the device has been stringency washed with 10 mM NaCl solution. A substantial difference in current was measured from the device with complementary and single-base mismatched DNA samples.
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Hsieh, Hsin-Hsien, and 謝信賢. "Synthesis of modified anchoring molecules for biochip substrate." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/02316128159689827403.
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碩士<br>淡江大學<br>化學學系碩士班<br>103<br>Our laboratory has developed and reported a series of thiophene-based compounds which were synthesized and employed as biolinkers of biochip. Due to π-electron conjugation property of mutiple thiophene rings, bis-thiophene-based compounds display planar structure and conjugation which possess some great electron conductivity. The introduction of thiol functionality on thiophene can provide the covalent bond on gold metal surface to generate Self-Assembled Monolayer (SAM) by a spontaneously chemical reaction. This SAM can improve the detection sensitivity when it was introduced on the biochip application.
Therefore, we synthesized two kinds of mercapto-bis-thiophene-based molecules, and also modified carboxylic acid group on another terminal site of thiophene ring.
These new organic compounds may be able to apply on the biochip research and act as the linkers between substrate and detecting probe .
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Chen, Hei-Wen, and 陳海雯. "Development of biochip for rapid detection of Leptospira." Thesis, 2015. http://ndltd.ncl.edu.tw/handle/62003709508449254714.
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碩士<br>中臺科技大學<br>醫學工程暨材料研究所<br>103<br>Leptospirosis is a worldwide zoonotic disease caused by pathogenic spirochetes of the genus Leptospira. This disease is infected by animal excrement, water, soil containing Leptospira coming into contact with breaks in the skin, eyes, mouth, or nose. Clinical symptoms range from a self-resolving acute undifferentiated febrile illness to severe, sometimes fatal disease with renal failure, jaundice, hemorrhage (particularly affecting the lungs), and vascular collapse. WHO believes the leptospirosis death rate may be between 5% to 25% of infected patients. The gold standard method of Leptospirosis diagnosis is the micro-agglutination test (MAT) that is time consuming. New molecular diagnostic methods, including polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) have high sensitivity and provide fast diagnosis. However, they require high-tech equipments and experts. Except the molecular diagnostic methods, one possible method is to directly capture Leptospira from samples with a slide which is coated specific antibodies. However, it is difficult to implement because the Leptospire is very active. The goal of this study is to develop a rapid and simple detection method for capturing Leptospira using a special designed chamber assembled with a funnel-shaped chamber made with Polydimethylsiloxane (PDMS) and a cover slide. The positive serum was coated on the bottom of the chamber. Using a horizontal centrifugal, the bacteria capture can be reached in a short time. The operation of the antibody coating is time consuming, and it needs a considerable quantity of materials. This study is expected to contribute to the diagnosis and research of the Leptospirosis.
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Chiang, Cheng-Lung, and 江政隆. "Defect Detection of Biochip with Low Contrast Image." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/56965183694383453040.
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碩士<br>國立臺灣大學<br>機械工程學研究所<br>104<br>In recent years, biotech industry, regarded as one of the key industries, has been developed rapidly. According to the survey made by TrendForce, biotech industry will account for 14% of GDP in Taiwan, and the market of biochip will be about 26 billion USD in 2020. Biochip technology is an important technology of biotech industry. Although many companies pursue high quality biochips, some of these companies still depend on human eyes to inspect defects, which causes lots of personnel cost and testing personnel making mistakes due to fatigue. If Automatic Optical inspection could be apply to quality control of biochips, it will not only reduce the cost but also improve the effectiveness and the accuracy of inspection.
In this research, the edges of different features are not obvious because of the contrast is very low, making it hard to set threshold to segment different features. This thesis proposes a novel algorithm for biochips with low contrast image, using several image processing techniques based on thresholding、edge detection、hough transform for image segmentation, and local thresholding for defect detection. The algorithm improves the success rate and reduces the time cost of defect detection.
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Lu, Yan-chih, and 盧彥池. "Using Surface Micromachining Implement Single Cell Biochip Platform." Thesis, 2007. http://ndltd.ncl.edu.tw/handle/88571566571813323185.
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碩士<br>國立成功大學<br>奈米科技暨微系統工程研究所<br>95<br>Patch clamp is a well-developed electrophysiological recording technique used to study ion channel function and regulation. The conventional method of performing patch clamp technique employs a glass micropipette onto the cell by manual manipulation. Despite this technique is extremely sensitive and information-rich, but requires a highly-skilled operator and is limited in throughput. Voltage clamp that determine the behavior of the ion channel conductance responsible for the generation of the action potential is the another method to record the flow of ionic current across the cell membrane. The method is held a constant membrane potential while the ionic current flowing through the membrane is measured.
This research continue the achievement of our laboratory. We not only replace traditional patch clamp of glass electrode by patch clamp chip, but also improve its efficacy, field and cost down. In this research we reduce the patch hole below 1μm using the method of changing structure material, process flow, and an additional process of BOE dipping. Then the accurate rate could be arised. By this we also reduce the whole structure thickness of the chip identically which can make local series resistance lower and stablely. Besides in the process we cancel the BOSCH ICP that has expensive patent cost, and replace bulk micromachining process by surface micromachining like process to raise filed, hence this chip would have much more competition potential.
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Yi-Te, Lin, and 林奕德. "Study of Design of Enzyme-Linked -Immunosorbent-Assay Biochip." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/87331062443787556755.
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Huang, Hsin-Ming, and 黃信銘. "A Study on Biochip Technology for Medical Treatment Application." Thesis, 2003. http://ndltd.ncl.edu.tw/handle/58627184792204665633.
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碩士<br>國立臺北科技大學<br>冷凍與低溫科技研究所<br>91<br>Biotechnology is known as a major science and technology by human in the 21st century. The development will significantly affect the living structure of human and also becomes a current competition at the high technology of developed countries in the world. One of an important development of technology is to integrate the microelectronics, biochemistry and electro-optical of biochip technology. The principle of molecular biology, gene information and analytical chemistry, etc. are applied to proceed the design. Using glass, silicon chip and macromolecule filter membrane as the based material can comply with the micro electro-mechanical system, automation or other precise manufacture technologies to make the high technology devices. Therefore, the major purpose of the thesis is to understand the various characteristic technology of biochip and design a biochip system to use the property of the body organ function concentrating in the chip. To understand the medicines use in body of various conditions and then help the medicines of research and development for medical treatment application. With the mature biochip technology, the ink-jet printing technology can quickly produce the various biochips. Applying this thesis of the designed biochip system can rapidly manufacture the products and reduce the equipment cost.
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Chen, Yung-Chang, and 陳永昌. "A study of Biochip with Multi-Channel Fluorescent Detection." Thesis, 2008. http://ndltd.ncl.edu.tw/handle/03239225186886497674.
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碩士<br>國立臺灣海洋大學<br>電機工程學系<br>96<br>In this article, MEMS technology processes are used to fabricate microfluidic chip. Lithography technology is used to make master mold for microfluidic base silicon substructure, and microfluidic chip is made through mold transfer technology. In the experiment, we used SU-8 thick photoresist to mold-transfer technology can achieve the minimum line width of 100um and thickness of 120μm.The microfluidic chip is used to the front of optical fiber coupling area and in order to enhance the fluorescence signal value, the curvature of lens in microfluidic chip is designed to 135 degrees .
Use Edwardsiella and Aeromonas as the standard sample and probe with label different fluorescence dye to DNA hybridization. In the fluorescence detection aspect, we have coupled the laser light source to the 1 to 4 tube optical fiber through objective lens, picks the side illumination to exciting the microfluidic chip, by way of experimental group AT89C2051 switch circuit, and control group PLC selection multi-channel switch signal fluorescence sample, we use buffer solution and connect micropump to wash biological sample.
Finally, we use multi-channel fluorescence switch signal system can measure several biological samples in the same time indeed through integrate system. When the flow rate is 15 ul/min, the variation of fluorescence voltage is small, to know whether DNA hybridization succeed by using DNA hybridization probe in real-time.
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Kuo, Shin-Hung, and 郭信宏. "Development of Transparent Biochip Platform for Patch Clamp Technology." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/15388654219936000560.
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碩士<br>國立成功大學<br>電機工程學系碩博士班<br>97<br>The single cell study is crucial for tissue engineering, drug screening, and DNA expressions. However, it is so difficult to get the ion channel activity by whole cell clamp. Therefore, how to release the difficulty in patching clamp for the world wide use in any lab becomes a hot issue now.
Biochip fabricated by N/MEMS techniques is one alternative to make it come true. The transparent single cell biochip platform is manufactured in poly-dimethylsioxane (PDMS) by soft lithography technology from a microfabricated silicon master to achieve high throughput on patch clamping. The silicon masters with three levels pillar shape structure array are fabricated via three times photolithography and inductive coupled plasma-reactive ion etching (ICP-RIE) processes. A high yield nested metal masks approach is used in order to transfer the desirable 1 μm patterns precisely from photo mask to the silicon substrate. Surface roughness is reduced below 1 μm by optimizing the ICP-RIE process parameters. The silicon master is suitable to be reused many times with using PDMS nondestructive releasing process. The fabricated PDMS micro platforms contain precise apertures of 1-2 μm diameters. The fabrication is sufficiently simple and economical by using soft lithography technologies.
In the impedance measurement with patch-clamp equipment, the open resistance and capacitance of PDMS chip with 1.5 μm aperture are 1.81 MΩ and 1.6 pF, respectively. Based on the performance, the high-throughput characteristic and possibility of automation on patch clamping, these PDMS chips have a large potential to replace the traditional glass pipettes on multiple patch clamp recordings. The breakthrough is coming soon to push DNA expressions theory forward with a great step.
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Hsiao, Hung-Yu, and 蕭宏宇. "Fabrication and Testing of Micro Gear and Polymeric Biochip." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/42796908539683730444.
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碩士<br>大葉大學<br>機械工程研究所碩士班<br>94<br>The greatest advantage of micro injection molding is that it can mass produce items rapidly and it is cheap. In this experiment, we manufacture items with micro-features and micro-channels by IM and hot embossing molding.LIGA techniques use high- strength X-ray to etch, and this technique went against us to research, because they need a lot of cost. As LIGA techniques are getting well, gradually. The study use LIGA-Like techniques, Micro Electro Mechanical System and micromachining technique put together to manufacture micro fluid biochip and micro gear and to experiment by injection molding machine. The study use exchangeable insets which micro structures size are ten to hundred micrometers. The structures size of insets and product measure by CCD and α-step. The study to expect to manufacture micro gear and biochip of micro fluid-channels by injection molding machine and hot-embossing machine are good and testing result well. Finally, hope to establish reference background in micro gear and biochip of micro fluid-channels by injection molding and hot-embossing area.
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Hsiao-Ting, Wu, and 吳曉婷. "Application of Synthetic Peptide in Biochip for Gas Sensing." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/50464293541511278235.
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碩士<br>輔仁大學<br>化學系<br>94<br>The mammalian olfactory organs can distinguish different kind of odorant molecules. When olfactory receptor in the nasal epithelium is activated by odorant molecules can stimulate the formation of cAMP, then open ion channels, and transmit olfactory signals to the brain. The olfactory receptor gene has already been cloned, and the amino acid sequence of the olfactory receptor has been recognized. The literature suggested that the odorant binding domain in olfactory receptors might be related to the specific amino acid sequence of the extracellular loop and transmembrane domain.
In this study, a series of synthetic peptides with specific amino acid sequence of olfactory receptor are prepared with the solid phase peptide synthesis method. The synthetic peptides are coated onto the surface of a piezoelectric electrode (PE). The PE biochip serves as a signal transducer to determine the binding affinity of synthetic peptides and odorants.
The result of these studies can determine the binding specificity and selectivity of synthetic peptides with odorants. The result can use for further development of biochips for odorant sensing tools in medicine, food, environmental, pesticide and cosmetic.
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Lee, Ching-Fang, and 李靜芳. "Application of synthetic peptide in biochip for odorant detection." Thesis, 2006. http://ndltd.ncl.edu.tw/handle/16252835711822585837.
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碩士<br>輔仁大學<br>化學系<br>94<br>The mammalian olfactory system can recognize and distinguish large number of different odorant molecules. The odor discrimination occurs during the association of odorous ligands with specific receptors
olfactory sensory neurons. The receptors protein plays an important role in cell signaling. Each olfactory receptor neuron expresses only one olfactory receptor protein. Each odor activates a unique combination of olfactory neurons allowing the brain to distinguish smell. The gene family of olfactory receptors have been cloned and characterized. The literature reports suggested that the extracellular 1oop and transmembrane domain might be the major part of the odorant-binding domain in olfactory receptors.
To mimic the olfactory sensing system,a series of synthetic peptides are prepared with the solid-phase peptide synthesis protocols mimicking the extracellular loop binding site of olfactory receptor protein to odorant. The synthetic peptide are coated onto the surface of a piezoelectric (PZ) electrode for target odorant binding assays. The PZ crystal are serve as a singal transducer to determine the binding affinity of synthetic peptides and odorants.
The result of these studies would be used for further development of biochips for odorant sensing tools in environmental,food,and medical sample analysis.
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Chang, Ko-Hsin, and 張可欣. "Exploring biochip as a tool for blood glucose determination." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/20027371146421553502.
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碩士<br>國立交通大學<br>應用化學系<br>91<br>Exploring biochip as a tool for blood glucose determination
Student: Ko-Hsin Chang Advisor : Dr. Yaw-Kuen Li
Department of Applied Chemistry
National Chiao Tung University
Abstrate
To build an accurate, fast and user-friendly system for diabetes is our main purpose of this study. A novel blood sugar detection system has been developed by integrating a CMOS chip with biochemical catalysis. The precise glucose concentration is calculated based on the standard calibration curve and the initial rate of the coupling enzymatic reaction catalyzed by glucose oxidase and peroxidase. For preliminary detection, the CMOS chip was integrated with a commercial A/D converter and the output signal was further collected and processed by PC. With this system, the glucose concentration can be accurately detected in the range of 0.1 mM to 25 mM. A second generation of chip was further designed by adding the signal amplification feature to enhance the output signal. A stand-alone system is further built and tested on its function. Although the proto-type of this system needs to be improved, it is the first CMOS-base glucose detection system. In addition, the catalytic function of glucose dehydrogenease is also under development for glucose detection.
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Chiu, Hsin-Jou, and 邱欣柔. "A Study of Developing Directions of Taiwan Biochip Industry." Thesis, 2002. http://ndltd.ncl.edu.tw/handle/44784108602865208022.
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碩士<br>國立交通大學<br>科技管理所<br>90<br>Biochips manufacturing requires knowledge from cross-disciplinary fields including biology, chemistry, electronics, micro mechanics, and material science etc. It has the potential to analyze a large number of genome quickly and inexpensively.
This research investigated the manufacturing technologies, the applications, and the current status of domestic and foreign biochip companies. The pioneering biochip company, Affymetrix, was analyzed as a case study. It’s development history, product lines, M&A strategies, and most importantly, the patent protections and dispute litigations were thoroughly studied. It could serve as a valuable guiding post for every biochip company to benchmark against.
The leading biochip companies in the world are building SNP databases, which would be essential to the development of new drugs as well as customized drugs. Developing biochips requires a large amount of capital and a relatively long period. Therefore, this research recommends domestic biochip companies establish strategic alliances with foreign biochip companies with patent rights. Moreover, Taiwan government sponsored biochip R&D organizations and private companies should join efforts to make biochip industry a success in Taiwan.
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Chen, Cheng-Hasio, and 陳宸効. "Optics Simulation and Performance Analysis on Dielectrophoretic Biochip Instrument." Thesis, 2009. http://ndltd.ncl.edu.tw/handle/28064371881321842406.
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碩士<br>國立屏東科技大學<br>生物機電工程系所<br>97<br>Dielectrophoretic biochip can manipulate biological cell and bacteria. Be used in dairy products rapid detection of bacteria. This thesis is intended to match dielectrophoretic biochip , developing a platform for rapid detection apply in ranch. On research use a microscope for the observation biochip and positioning system(stepper motor). Instrument the development of design and test. Microscope test: objective lens selected , objective lens work distance , objective lens resolution. Employ Zemax simulation of optical software. Simulation number aperture of objective and relationship between light intensity. Employ TracePro simulation of optical software, simulation sample is dielectrophoretic biochip, the impact of light intensity and the ray-tracing. Stepper motor positioning system for performance analysis: resolution , max speed , positioning accuracy , and location reproducibility.
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Liu, Yuan-Hsi, and 劉元璽. "DESIGN AND FABRICATION BIOCHIP TO SEPARATE ERYTHROCYTE AND LEUKCYTE." Thesis, 2016. http://ndltd.ncl.edu.tw/handle/63057445586701326664.
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碩士<br>大同大學<br>機械工程學系(所)<br>104<br>Along with the improvement in the quality of life in recent years, the number of obese patients has been increasing rapidly, and with respect to health care, real-time monitoring has become an indispensable technique. This study therefore aimed to investigate the operation of front-end separation in the detection, with the function of separation chips focusing on “fast”, “simple” and “convenient.” This thesis proposed the DLD erythrocyte and leukocyte separation chip, and through making use of lithography, it can easily and conveniently remove leukocytes in the blood.
This study proposed the DLD erythrocyte and leukocyte separation chip. PDMS was selected as its material, since PDMS, being biocompatible, will not cause the death of or changes in cells. In its production, SU-8 photoresist was used in making the mold, and PDMS in replica modeling. Oxygen plasma technology was applied in chip bonding to obtain the surface modification of PDMS.
Three flow rates, 5 μl/min、30 μl/min、60 μl/min, were tested to verify the efficiency of separating erythrocyte and leukocyte, with the results indicating the flow rate of 30 μl/min having the best leukocyte removal rate.
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Serrão, Ricardo Filipe Soares. "Monitoring cell cultures in real time in a biochip." Master's thesis, 2019. http://hdl.handle.net/10362/95872.
Full textAbstract:
Drug screening is a very important procedure in the approval of drugs for cancer treatment. This process is generally carried out using in vitro or in vivo models that aren’t very efficient due to the non-reproducibility of the cellular and/or tissue microenvironment and ethical issues due to the use of animal models. Additionally, drug approval is a process that could last 10 to 15 years, too much time when therapy is required with urgency.
Microfluidic structures can address such issues, decreasing the time per assay, as well as decreasing the quantity of reagents used and the volume of waste generated, thus decreasing the costs. Also, due to the generation of concentration gradients inside a microfluidic device, it mimics the microenvironment characteristic of conventional cell culture.
In this work, a reproducible cell culture of HCT-116, a human colon cancer cell line, is successfully grown inside a microfluidic device for a posterior exposure to anti-cancer drugs. The cell viability, detected through staining the DNA with fluorophores, is on average 90%.
To monitor the cell death via exposure to drugs, a specific cell death biomarker, adenylate kinase (AK), is detected inside a microfluidic device using a photomultiplier and a fluorescence microscope in a chip-based immunoassay. AK concentrations near the concentrations of the enzyme released by dead cells were detected with the immunoassay by concentrating the AK in packed agarose beads inside de microfluidic structure.
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lin, ji-fu, and 林紀甫. "Biochip fabrication of action potential measurement for single cells." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/49810486079887131772.
Full textAbstract:
碩士<br>國立成功大學<br>微機電系統工程研究所<br>93<br>Patch clamp is a well-developed lelectrophysiological recording technique used to study ion channel function and regulation. The conventional method of performing patch clamp technique employs a glass micropipette onto the cell by manual manipulation. Despite this technique is extremely sensitive and information-rich, but requires a highly-skilled operator and is limited in throughput. Voltage clamp that determine the behavior of the ion channel conductances responsible for the generation of the action potential is the another method to record the flow of ionic current across the cell membrance. The method is held a constant membrane potential while the ionic current flowing through the membrane is measured.
The thesis presents cell-platforms and etcing pores on silicon by using soft lithography and etching technique insteading of conventional glass micropipette recording method. we successfully demonstrated practicability of the process by the cells adhered on the platform and the etching pores on the silicon chip by using ICP. The less laborious manipulation、time saving and high sampling throughput will be expected .In the future, we can automatically real time controlled and changed in response to the cell’s physiologic characteristics measured by ionic channel activities.