Academic literature on the topic 'Biocontrol; Fungi inhibiting growth; Tree pathogen'

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Journal articles on the topic "Biocontrol; Fungi inhibiting growth; Tree pathogen"

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Nisa, Miratun, Fitratul Aini, and Hasna Ul Maritsa. "AKTIVITAS ANTAGONISTIK BAKTERI SELULOLITIK ASAL RHIZOSFER KELAPA SAWIT (Elaeis guineensis Jacq.) TERHADAP Ganoderma boninense Pat." Al-Kauniyah: Jurnal Biologi 13, no. 1 (April 30, 2020): 9–19. http://dx.doi.org/10.15408/kauniyah.v13i1.11704.

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AbstrakPenyakit busuk pangkal batang pada kelapa sawit (Elaeis guineensis Jacq.) disebabkan oleh Ganoderma boninense Pat. Bakteri selulolitik dari rizosfer kelapa sawit dapat dijadikan agen hayati dalam menghambat pertumbuhan G. boninense. Penelitian ini bertujuan untuk mendapatkan bakteri selulolitik dari rizosfer kelapa sawit dan menentukan efektivitasnya dalam menghambat pertumbuhan G. boninense. Tahap penelitian meliputi pengambilan sampel dari rizosfer kelapa sawit, isolasi, pemurnian, uji aktivitas selulolitik, dan uji daya hambat terhadap G. boninense serta identifikasi isolat bakteri yang potensial. Indeks selulolitik bakteri ditentukan dengan pewarnaan congo red 0,1%. Aktivitas penghambatan dilakukan dengan menentukan persentase daya hambat bakteri dalam menghambat G. boninense. Uji efektivitas dengan membandingkan hasil uji T pada taraf 5%. Hasil menunjukkan bahwa dari rizosfer kelapa sawit diperoleh 19 isolat bakteri selulolitik dengan indeks selulolitik tertinggi 4,38 pada isolat LBS1. Berdasarkan uji T dari efektivitas bakteri selulolitik terhadap G. boninense menunjukkan 6 isolat bakteri (LBS3, LBS4, DBS1, DBS7, SBS2 dan SBS6) memiliki nilai efektif atau berpotensi sebagai antagonis dengan persentase daya hambat tertinggi sebesar 40,17% isolat DBS1 yang merupakan genus Flavobacterium. Harapan kedepannya, bakteri selulolitik yang diperoleh dapat dijadikan agen biokontrol terhadap G. boninense.Abstract Basal stem root disease in oil palm tree (Elaeis guineensis Jacq.) is caused by Ganoderma boninense Pat. infection. Cellulolytic bacteria from rhizosphere can be used as agents to inhibit G. boninense growth as pathogenic fungi. Purpose of the research is to obtain cellulolytic bacteria from oil palm tree rhizosphere and to determine their effectiveness in inhibiting G. boninense growth. The research stages included sampling from the oil palm tree rhizosphere, isolation, purification, cellulolytic activity test and dual culture test against G. boninense and identification of effective isolates. The cellulolytic index of bacteria was determined by congo red 0.1%. Antagonistic test was conducted to determine persentation of isolate bacteria in inhibit G. boninense and effectiveness test by comparing result of T test at the level 5%. The results showed that there are 19 isolates of cellulolytic bacteria from oil palm tree rhizosphere with highest cellulolytic index 4.38 (isolates LBS1). Based on T test of the effectiveness cellulolytic bacteria against G. boninense showed 6 isolates (LBS3, LBS4, DBS1, DBS7, SBS2, and SBS6) had effective values or potential antagonists with highest percentage inhibition 40.17% isolate DBS1 genus of Flavobacterium. Hope that in the future, cellulolytic bacteria obtained can be used as biocontrol agents for G. boninense.
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Costa, Daniela, Rui M. Tavares, Paula Baptista, and Teresa Lino-Neto. "Cork Oak Endophytic Fungi as Potential Biocontrol Agents against Biscogniauxia mediterranea and Diplodia corticola." Journal of Fungi 6, no. 4 (November 14, 2020): 287. http://dx.doi.org/10.3390/jof6040287.

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An increase in cork oak diseases caused by Biscogniauxia mediterranea and Diplodia corticola has been reported in the last decade. Due to the high socio-economic and ecologic importance of this plant species in the Mediterranean Basin, the search for preventive or treatment measures to control these diseases is an urgent need. Fungal endophytes were recovered from cork oak trees with different disease severity levels, using culture-dependent methods. The results showed a higher number of potential pathogens than beneficial fungi such as cork oak endophytes, even in healthy plants. The antagonist potential of a selection of eight cork oak fungal endophytes was tested against B. mediterranea and D. corticola by dual-plate assays. The tested endophytes were more efficient in inhibiting D. corticola than B. mediterranea growth, but Simplicillium aogashimaense, Fimetariella rabenhorstii, Chaetomium sp. and Alternaria alternata revealed a high potential to inhibit the growth of both. Simplicillium aogashimaense caused macroscopic and microscopic mycelial/hyphal deformations and presented promising results in controlling both phytopathogens’ growth in vitro. The evaluation of the antagonistic potential of non-volatile and volatile compounds also revealed that A. alternata compounds could be further explored for inhibiting both pathogens. These findings provide valuable knowledge that can be further explored in in vivo assays to find a suitable biocontrol agent for these cork oak diseases.
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Karličić, Vera, Jelena Jovičić-Petrović, Veljko Marojević, Milica Zlatković, Saša Orlović, and Vera Raičević. "Potential of Trichoderma spp. and Pinus sylvestris Bark Extracts as Biocontrol Agents against Fungal Pathogens Residing in the Botryosphaeriales." Environmental Sciences Proceedings 3, no. 1 (November 12, 2020): 99. http://dx.doi.org/10.3390/iecf2020-07960.

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Botryosphaeriales represent a diverse order of fungal pathogens of various woody plant species. In Serbia, these fungi are important pathogens of forest, ornamental, and fruit trees causing die-back, cankers, leaf blights, fruit, and root rot. The aim of this study was to evaluate the antifungal activity of Pinus sylvestris bark extracts and Trichoderma spp. against Botryosphaeria dothidea, Dothiorella sarmentorum, and Neofusicoccum parvum (Ascomycota, Botryosphaeriales) isolated from Picea abies, Thuja occidentalis, and Prunus laurocerasus trees planted in urban areas in Serbia. Bark extracts were prepared in water solution at two temperatures (80 and 120 °C). The extracts were tested using two concentrations (20% and 30%). Moreover, two Trichoderma isolates obtained from P. sylvestris bark were tested against Botryosphaeriales and their antagonistic potential was estimated in vitro using a confrontation test. Mycelial growth of B. dothidea and D. sarmentorum was significantly inhibited in the presence of bark extracts, while N. parvum showed no growth inhibition. Botryosphaeria dothidea growth was inhibited by 35 to 39% in the case of 20% extracts and by 39 to 44% in the case of 30% extracts. The growth inhibition of D. sarmentorum was between 48% and 56% in the case of 20% extracts and between 53% and 60% in the case of 30% extracts. The two Trichoderma isolates showed antifungal activity against the selected pathogens. An isolate BKG 4 showed the highest inhibition level, and it inhibited the growth of B. dothidea, D. sarmentorum, and N. parvum by 85%, 75%, and 62%, respectively. Preliminary results suggest that both P. sylvestris bark extracts and Trichoderma spp. could be used as biocontrol agents against B. dothidea, D. sarmentorum, and N. parvum, and this should be a further studied.
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Bizos, Georgios, Efimia M. Papatheodorou, Theocharis Chatzistathis, Nikoletta Ntalli, Vassilis G. Aschonitis, and Nikolaos Monokrousos. "The Role of Microbial Inoculants on Plant Protection, Growth Stimulation, and Crop Productivity of the Olive Tree (Olea europea L.)." Plants 9, no. 6 (June 12, 2020): 743. http://dx.doi.org/10.3390/plants9060743.

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The olive tree (Olea europaea L.) is an emblematic, long-living fruit tree species of profound economic and environmental importance. This study is a literature review of articles published during the last 10 years about the role of beneficial microbes [Arbuscular Mycorrhizal Fungi (AMF), Plant Growth Promoting Rhizobacteria (PGPR), Plant Growth Promoting Fungi (PGPF), and Endophytes] on olive tree plant growth and productivity, pathogen control, and alleviation from abiotic stress. The majority of the studies examined the AMF effect using mostly Rhizophagus irregularis and Glomus mosseae species. These AMF species stimulate the root growth improving the resistance of olive plants to environmental and transplantation stresses. Among the PGPR, the nitrogen-fixing bacteria Azospirillum sp. and potassium- and phosphorous-solubilizing Bacillus sp. species were studied extensively. These PGPR species were combined with proper cultural practices and improved considerably olive plant’s growth. The endophytic bacterial species Pseudomonas fluorescens and Bacillus sp., as well as the fungal species Trichoderma sp. were identified as the most effective biocontrol agents against olive tree diseases (e.g., Verticillium wilt, root rot, and anthracnose).
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Senouci, Hanane, Nassira G. Benyelles, Mohammed EA Dib, Jean Costa, and Alain Muselli. "Ammoides verticillata Essential Oil as Biocontrol Agent of Selected Fungi and Pest of Olive Tree." Recent Patents on Food, Nutrition & Agriculture 11, no. 2 (September 18, 2020): 182–88. http://dx.doi.org/10.2174/2212798410666190619110251.

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Background: Ammoides verticillata Briq is an aromatic and medicinal herb that has been widely used in folk medicine for treatment of several types of pathologies such as respiratory problems, colds, fever, headache, gastric disorders and renal infections. Objective: Essential oils have been subjected of numerous works for their antimicrobial and insecticidal properties which have been valued by hundreds of publications and patents. The main objective of this work was to evaluate the chemical composition, and the in vitro and in vivo antifungal, and insecticidal properties of essential oil of Ammoides verticillata. Methods: In this work, the air-dried aerial parts of Ammoides verticillata were hydrodistilled in a Clevenger-type apparatus. The essential oil isolated was analyzed using gas chromatography (GC) and mass spectrometry (GC/MS). The in vitro antifungal activity of the essential oil was investigated against four plant fungi using radial growth technique. The effect of the essential oil on disease development of olive caused by Penicillium crustosum and Aspergillus niger in the in vivo conditions. The fumigation test of the essential oil was evaluated against L3 stages, pupas and adults of Bactrocera oleae known as the olive fly. Results: The essential oil of A. verticillata was characterized mainly by carvacrol (44.3%), limonene (19.3%) and p-cymene (19.2%). The result of in vitro antifungal activity of essential oil showed an interesting antifungal inhibition against Alternaria alternata and Fusarium solani strains with percentage inhibition of 89%. Furthermore, oil of A. verticillata has demonstrated promising in vivo antifungal activity to control infections of olives caused by Aspergillus niger and Penicillium crustosum. In addition, the essential oil exhibited fumigation toxicity against Bactrocera oleae with mortality percentage of 100% at 2 μL/L air. Conclusion: The results showed that essential oil of A. verticillata has interesting biological activities, which suggests that oil have the potential to be used as biocide to protect olives of pathogenic fungi and pests.
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Yu, Menghao, Guiying Zhang, Jiasong Jiang, Liangcheng Du, and Youfu Zhao. "Lysobacter enzymogenes Employs Diverse Genes for Inhibiting Hypha Growth and Spore Germination of Soybean Fungal Pathogens." Phytopathology® 110, no. 3 (March 2020): 593–602. http://dx.doi.org/10.1094/phyto-09-19-0356-r.

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Lysobacter enzymogenes strain C3 (LeC3) is a potential biocontrol agent for plant diseases caused by fungi and oomycetes. Understanding the interaction between LeC3 and soybean pathogens at the molecular level could help improve its biocontrol efficacy. In this study, we obtained mutants with decreased abilities in inhibiting hypha growth of the white mold pathogen Sclerotinia sclerotiorum. Insertion sites for 50 mutants, which no longer inhibited S. sclerotiorum hypha growth in dual cultural assay, were determined and seven mutants were selected for further characterization. These seven mutants also completely lost their abilities in suppressing spore germination of Fusarium virguliforme, the causal agent of soybean sudden death syndrome. Furthermore, mutation of the seven genes, which encode diguanylate cyclase, transcriptional regulators from the TetR family, hemolysin III family channel protein, type IV secretion system VirB10 protein, phenol hydroxylase, and phosphoadenosine phosphosulfate reductase, respectively, led to reduced production or secretion of four extracellular enzymes and heat-stable antifungal factor (HSAF). These results suggest that these seven genes play important roles in L. enzymogenes in suppressing hypha growth and spore germination of fungal pathogens, probably by influencing production or secretion of extracellular enzymes and HSAF.
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Hardiyanti, Siti, Bonny Poernomo Wahyu Soekarno, and Titiek Siti Yuliani. "Kemampuan Mikrob Endofit dan Rizosfer Tanaman Karet dalam Mengendalikan Rigidoporus lignosus." Jurnal Fitopatologi Indonesia 13, no. 5 (June 26, 2018): 153. http://dx.doi.org/10.14692/jfi.13.5.153.

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Rigidoporus lignosus is the most important pathogen of rubber tree which causes white root rot disease. The use of antagonistic microbe is recommended to control this pathogen. This research was conducted to isolate endophytic and rhizospheric microbes, and to study their ability to inhibit growth of R. lignosus. Research consisted of isolation of endophytic and rhizospheric microbes, pathogenicity test, in vitro and in vivo assays, growth promotion assays, and identification. There were 99 isolates of bacteria and 18 isolates of fungi isolated from the root and rhizosphere of rubber trees. In vitro and in vivo assay showed that 2 bacterial isolates, i.e. endophytic bacteria ME8, and rhizospheric bacteria MR3; and 3 fungal isolates, i.e. endophytic fungi CB8, CB6, and CL3 were able to inhibit the growth of R. lignosus. Endophytic bacteria ME8 showed the ability of solibilizing phosphate and fixing nitrogen. Rhizospheric bacteria MR3 showed the ability of solubilizing phosphate. The isolates CB6 and CL3 were very similar with Chaetomium sp. and Penicillium sp., respectively based on morphological characters; while CB8 was identified as mycelial sterile. Based on 16S rRNA sequences, endophytic bacterium ME8 and rhizospheric bacteria ME3 were identified as Bacillus siamensis B268 and B. amylolyquefaciens BCRh10, respectively. Endophytic and rhizospheric microbes isolated from rubber trees has the potency as biocontrol agents of R. lignosus.
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Velázquez-Robledo, R., H. A. Contreras-Cornejo, L. Macías-Rodríguez, A. Hernández-Morales, J. Aguirre, S. Casas-Flores, J. López-Bucio, and A. Herrera-Estrella. "Role of the 4-Phosphopantetheinyl Transferase of Trichoderma virens in Secondary Metabolism and Induction of Plant Defense Responses." Molecular Plant-Microbe Interactions® 24, no. 12 (December 2011): 1459–71. http://dx.doi.org/10.1094/mpmi-02-11-0045.

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Trichoderma virens is a ubiquitous soil fungus successfully used in biological control due to its efficient colonization of plant roots. In fungi, 4-phosphopantetheinyl transferases (PPTases) activate enzymes involved in primary and secondary metabolism. Therefore, we cloned the PPTase gene ppt1 from T. virens and generated PPTase-deficient (Δppt1) and overexpressing strains to investigate the role of this enzyme in biocontrol and induction of plant defense responses. The Δppt1 mutants were auxotrophic for lysine, produced nonpigmented conidia, and were unable to synthesize nonribosomal peptides. Although spore germination was severely compromised under both low and high iron availability, mycelial growth occurred faster than the wild type, and the mutants were able to efficiently colonize plant roots. The Δppt1 mutants were unable of inhibiting growth of phytopathogenic fungi in vitro. Arabidopsis thaliana seedlings co-cultivated with wild-type T. virens showed increased expression of pPr1a:uidA and pLox2:uidA markers, which correlated with enhanced accumulation of salicylic acid (SA), jasmonic acid, camalexin, and resistance to Botrytis cinerea. Co-cultivation of A. thaliana seedlings with Δppt1 mutants compromised the SA and camalexin responses, resulting in decreased protection against the pathogen. Our data reveal an important role of T. virens PPT1 in antibiosis and induction of SA and camalexin-dependent plant defense responses.
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Zhang, Xifen, Yaochen Wang, Xiaobin Han, Jianyu Gou, Wei Li, and Chengsheng Zhang. "A Novel Bio-Fertilizer Produced by Prickly Ash Seeds with Biochar Addition Induces Soil Suppressiveness against Black Shank Disease on Tobacco." Applied Sciences 11, no. 16 (August 6, 2021): 7261. http://dx.doi.org/10.3390/app11167261.

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A novel bio-fertilizer, produced from prickly ash seeds (PAS), Bacillus subtilis and biochar, was evaluated for its disease-preventing potential on tobacco black shank caused by Phytophthora nicotianae. The results showed that biochar promoted the growth of Tpb55 in PAS and increased the pH of the organic fertilizer. The final concentration of B. subtilis could reach 1.7 × 1010 cfu g−1 in the biological organic fertilizer (PBB) under the optimal medium under conditions of solid-state fermentation. PBB exhibited a strong fumigation effect on P. nicotianae, including inhibiting mycelium growth, reducing the disease severity and decreasing the pathogen population in rhizospheric soil. PBB treatment also could significantly increase the pH of acidified soil and improve soil nutrition content such as available K, alkali hydrolysable N and organic carbon. High-throughput pyrosequencing of 16S and 18S rRNA genes revealed that 4% PBB addition in soil had significant effects on the diversity and richness of fungi but not on that of bacteria. The microbial community structure was also shifted after PBB treatment. Some potentially beneficial microbes such as Bacillus, Mucor, Cunninghamella, Chitinophaga and Phenylobacterium were enriched, while potential pathogen Fusarium was significantly decreased. In conclusion, the agricultural waste PAS combined with biochar can replace soybean as a source for the production of biocontrol B. subtilis Tpb55, and the novel bio-fertilizer could effectively control tobacco black shank by pathogen inhibition, soil nutrient improvement and shifting the rhizomicrobial community.
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Vandana, Udaya Kumar, Jina Rajkumari, L. Paikhomba Singha, Lakkakula Satish, Hemasundar Alavilli, Pamidimarri D. V. N. Sudheer, Sushma Chauhan, et al. "The Endophytic Microbiome as a Hotspot of Synergistic Interactions, with Prospects of Plant Growth Promotion." Biology 10, no. 2 (February 1, 2021): 101. http://dx.doi.org/10.3390/biology10020101.

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The plant root is the primary site of interaction between plants and associated microorganisms and constitutes the main components of plant microbiomes that impact crop production. The endophytic bacteria in the root zone have an important role in plant growth promotion. Diverse microbial communities inhabit plant root tissues, and they directly or indirectly promote plant growth by inhibiting the growth of plant pathogens, producing various secondary metabolites. Mechanisms of plant growth promotion and response of root endophytic microorganisms for their survival and colonization in the host plants are the result of complex plant-microbe interactions. Endophytic microorganisms also assist the host to sustain different biotic and abiotic stresses. Better insights are emerging for the endophyte, such as host plant interactions due to advancements in ‘omic’ technologies, which facilitate the exploration of genes that are responsible for plant tissue colonization. Consequently, this is informative to envisage putative functions and metabolic processes crucial for endophytic adaptations. Detection of cell signaling molecules between host plants and identification of compounds synthesized by root endophytes are effective means for their utilization in the agriculture sector as biofertilizers. In addition, it is interesting that the endophytic microorganism colonization impacts the relative abundance of indigenous microbial communities and suppresses the deleterious microorganisms in plant tissues. Natural products released by endophytes act as biocontrol agents and inhibit pathogen growth. The symbiosis of endophytic bacteria and arbuscular mycorrhizal fungi (AMF) affects plant symbiotic signaling pathways and root colonization patterns and phytohormone synthesis. In this review, the potential of the root endophytic community, colonization, and role in the improvement of plant growth has been explained in the light of intricate plant-microbe interactions.
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Dissertations / Theses on the topic "Biocontrol; Fungi inhibiting growth; Tree pathogen"

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Rind, Bashiran. "Studies relating to biological control of Armillaria mellea." Thesis, University of Sheffield, 2001. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.369944.

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