Journal articles: 'Biocontrollers'

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Friedlander, Michael, Nora Weintraub, Arik Freedman, Joni Sheer, Zvi Snovsky, Jim Shapiro, and George Wm Kissil. "Fish as potential biocontrollers of Gracilaria (Rhodophyta) culture." Aquaculture 145, no. 1-4 (October 1996): 113–18. http://dx.doi.org/10.1016/s0044-8486(96)01350-6.

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Lemes, Camila G. C., Isabella F. Cordeiro, Camila H. de Paula, Ana K. Silva, Flávio F. do Carmo, Luciana H. Y. Kamino, Flávia M. S. Carvalho, Juan C. Caicedo, Jesus A. Ferro, and Leandro M. Moreira. "Potential Bioinoculants for Sustainable Agriculture Prospected from Ferruginous Caves of the Iron Quadrangle/Brazil." Sustainability 13, no. 16 (August 20, 2021): 9354. http://dx.doi.org/10.3390/su13169354.

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Biocontrol and plant growth-promoting bacteria (PGPB) are important agricultural bioinoculants. This study aimed to prospect new potential bioinoculants for a more sustainable agriculture from ferruginous caves of the Brazilian Iron Quadrangle. Culturable bacteria, from seven caves and one canga soil sample, were evaluated for biocontroller activity of the phytopathogens Xanthomonas citri subsp. Citri—Xcc306 (citrus canker), Fusarium oxysporum—Fo (fusariosis), and Colletotrichum lindemuthianum—Cl89 (bean anthracnose). The ability of the superior candidates to solubilize inorganic phosphate, fix nitrogen, and produce hydrolytic enzymes and siderophores was then analyzed. Out of 563 isolates, 47 inhibited the growth of Xcc306 in vitro, of which 9 reduced citrus canker up to 68% when co-inoculated with the pathogen on host plants. Twenty of the 47 inhibited Fo growth directly by 51–73%, and 15 indirectly by 75–81%. These 15 inhibited Cl89 growth in vitro (up to 93% directly and 100% indirectly), fixed nitrogen, produced proteases and siderophores, showed motility ability, produced biofilm, and all but one solubilized inorganic phosphate. Therefore, 15 (2.66%) bacterial isolates, from the genera Serratia, Nissabacter, and Dickeya, act simultaneously as biocontrollers and PGPBs, and could be important candidates for future investigations in planta as an alternative to minimize the use of pesticides and chemical fertilizers through sustainable agricultural management practices.

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Ramirez, Cecilia, Alverlan da Silva Araújo, Gilson Moura Filho, Fernando da Silva Rocha, Marylia Gabriella Silva Costa, and Maria de Fatima Silva Muniz. "Biocontrollers in the management of yam dry rot nematodes." Diversitas Journal 6, no. 1 (January 24, 2021): 24–35. http://dx.doi.org/10.17648/diversitas-journal-v6i1-1572.

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ABSTRACT: One of the main diseases affecting yam crops (Dioscorea spp.) in Brazil is the dry rot caused by Scutellonema bradys, Pratylenchus brachyurus and P. coffeae nematodes. The use of biological control agents is an auspicious procedure which has been tested in order to reduce losses by pathogens. The objective of this work was to evaluate the nematicidal activity in vitro and in vivo of commercial biological products on yam dry rot nematodes. Products based on Trichoderma harzianum (2.0 x 109 conidia mL-1) at dosages of 1.5 and 2.0 L 200 L-1 of water; Bacillus subtilis 20% - 1.0 x 1011 cfu g-1 + B. licheniformis 20% - 1.0 x 1011 cfu g-1 at 100 and 150 g 100 L-1; B. subtilis 200 g kg-1 + B. licheniformis 200 g kg-1 at 130 and 200 g 100 L-1; combination of rhizobacteria including Bacillus spp. and organic carbon at 5L and 7L 100 L-1; and the control (distilled water), were tested in in vitro assays on S. bradys or Pratylenchus sp. In experiments performed under greenhouse conditions, healthy seed tubers were planted in sterilized soil and thirty days later the soil was infested with a suspension of 1,000 specimens of a mixed population of S. bradys and P. coffeae. Then, after 30 days products based on B. subtilis 20% + B. licheniformis 20% - 150 g 100 L-1; T. harzianum 2 L 200 L-1 and rhizobacteria + organic carbon 7 L 100 L-1, at 100 mL per pot, were applied to the soil. Three months after planting, the percentage of sprouting of the seed tubers was evaluated and in the fifth month, the nematode population densities were determined. The sprouting of seed tubers was of 100% in all treatments. Bacillus subtilis 20% + B. licheniformis 20% and T. harzianum caused 89% and 61% mortality in S. bradys respectively, at the highest concentrations. In specimens of Pratylenchus sp., rhizobacteria + organic carbon exhibited 51% and 45% mortality at higher and lower concentrations, respectively. Under greenhouse conditions, B. subtilis 20% + B. licheniformis 20% and rhizobacteria + organic carbon were more effective in reducing nematode population densities, compared to the control. KEYWORDS: Dioscorea spp.; Scutellonema bradys; Pratylenchus sp.

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Quispe-Quispe, Edwin, Anthony A. Moreira-Morrillo, and Felipe Rafael Garcés Fiallos. "A review about biocontrollers of Phytophthora capsici and its impact on Capsicum plants: A perspective from outside to inside the plant." Scientia Agropecuaria 13, no. 3 (September 21, 2022): 275–89. http://dx.doi.org/10.17268/sci.agropecu.2022.025.

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Phytophthora capsici is an oomycete that causes various symptoms, such as root, neck, stem, fruit rot, and leaf blight, in different plant species, including the genus Capsicum. One of the tools to counteract this biotic problem, which may be more profitable and respectful to the environment in the long term, is using biocontrollers such as Bacillus, Pseudomonas, Streptomyces (bacteria), and Trichoderma (fungus). It seems that each of these microorganisms has different mechanisms that allow them to inhibit and reduce the growth of P. capsici, negatively affecting the development of sporangia, germination and motility of zoospores, and expansion of germ tube. Although this direct biocontrol action on the phytopathogen correlates with the reduction of symptoms in Capsicum plants or other plant species, this would also involve the activation of defense responses in plants against P. capsici induced by microorganisms. The activity of several enzymes related to the isoflavonoid pathways and reactive oxygen species, as well as the expression of different genes that encode proteins related to pathogenesis and other proteins that can activate the jasmonic acid, salicylic acid, or ethylene signaling pathways. Despite the few existing works related to the biochemical and molecular interaction of Capsicum–P. capsici–biocontroller, in this review, we outlined and elucidated the possible effects and metabolic pathways related to this tripartite pathosystem.

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Pereira, Guillermo, Nicole Roa, Diyanira Castillo-Novales, César Arriagada, Héctor Herrera, Marco Molina-Montenegro, and Cristian Atala. "Mycorrhizal fungi isolated from Chilean orchids as biocontrollers of the pathogen Rhizoctonia solani." Gayana. Botánica 78, no. 2 (December 2021): 113–20. http://dx.doi.org/10.4067/s0717-66432021000200113.

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Vejarano, Ricardo, and Angie Gil-Calderón. "Commercially Available Non-Saccharomyces Yeasts for Winemaking: Current Market, Advantages over Saccharomyces, Biocompatibility, and Safety." Fermentation 7, no. 3 (August 28, 2021): 171. http://dx.doi.org/10.3390/fermentation7030171.

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About 42 commercial products based on non-Saccharomyces yeasts are estimated as available on the market, being mostly pure cultures (79%), with a predominance of Torulaspora delbrueckii, Lachancea thermotolerans, and Metschnikowia pulcherrima. The others are multi-starter consortia that include non-Saccharomyces/Saccharomyces mixtures or only non-Saccharomyces species. Several commercial yeasts have shown adequate biocompatibility with S. cerevisiae in mixed fermentations, allowing an increased contribution of metabolites of oenological interest, such as glycerol, esters, higher alcohols, acids, thiols, and terpenes, among others, in addition to a lower production of acetic acid, volatile phenols, biogenic amines, or urea. Multi-starter inoculations are also reviewed here, which show adequate biocompatibility and synergy between species. In certain cases, the aromatic profile of wines based on grape varieties considered neutral is improved. In addition, several yeasts show the capacity as biocontrollers against contaminating microorganisms. The studies conducted to date demonstrate the potential of these yeasts to improve the properties of wine as an alternative and complement to the traditional S. cerevisiae.

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Melo R., Aslenis, Pedro Ariza, Sofía Lissbrant, and Adriana Tofiño. "Evaluation of agrochemicals and bioinputs for sustainable bean management on the Caribbean coast of Colombia." Agronomía Colombiana 33, no. 2 (May 1, 2015): 203–11. http://dx.doi.org/10.15446/agron.colomb.v33n2.49858.

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The sustainable expansion of bean cultivations requires technologies that do not limit their phyto-recovering properties. Therefore, the objective of this study was to propose agronomic management of conservation for bean cultivation considering the microbiological characteristics of two mega-environments of the Colombian Caribbean coast and the compatibility between agrochemicals and bioinputs. The methodology included rhizospheric microbe population counts, identification of phytopathogenic fungi in plant tissues and soils, compatibility studies of pesticides with biocontrollers, and determination of residual contents of pesticides in bean seeds. The microbial populations corresponded to those previously registered for the lower tropics, but with quantitative differences in the genera. Phytophthora, Colletotrichum and Fusarium were registered in the humid Caribbean, while Colletotrichum and Curvularia affected crops in the dry Caribbean. The Beauveria bioinput was not compatible with the evaluated agrochemicals, while Trichoderma was compatible with chlorpyrifos, thiabendazole and oxycarboxin. Metarhizium was compatible with glyphosate and oxycarboxin at 10% of the recommended dose. Lindane residues were found in the beans harvested at three of the studied locations. The combined use of agrochemicals and bioinputs on bean crops is feasible as long as the time of application of the latter is made according to the half-life of the chemical and the organic matter content of the soil is increased.

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Hong-Thao, Phan Thi, Nguyen Vu Mai-Linh, Nguyen Thi Hong-Lien, and Nguyen Van Hieu. "Biological Characteristics and Antimicrobial Activity of EndophyticStreptomycessp. TQR12-4 Isolated from EliteCitrus nobilisCultivar Ham Yen of Vietnam." International Journal of Microbiology 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/7207818.

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Ham Yen orange (Citrus nobilisLour) is the highly valuable commercial fruit of Vietnam. With the blooming of fruit production and farming area, this specialty crop is facing threats from several serious diseases; therefore the search for new effective biocontrollers is required to prevent the existing excessive use of fertilizers and plant protection chemicals. Endophytic actinomycetes are of great scientific interest due to their high potential of application in agriculture and pharmaceutical research. In this work, endophytic actinomycetes were isolated from a native orange species of Northeast mountainous province Tuyen Quang. Among 49 isolates obtained, the isolate TQR12-4 strongly inhibited test pathogensColletotrichum truncatum,Geotrichum candidum,Fusarium oxysporum, andF. udum. This isolate gave comparatively high biomass yields on different substrates, for example, carboxy methyl cellulose, starch, protein, and chitin, within a wide range of temperature from 15 to 45°C and pH from 4 to 10. Sequence analysis of 16S rDNA gene showed that TQR12-4 shared 99% similarity toStreptomyces prasinopilosus; however, it slightly differed from the latter in spore morphology and hence was named asStreptomycessp. TQR12-4. A thermostable antifungal substance of nonpeptide nature produced byStreptomycessp. TQR12-4 had MIC againstFusarium udumof 100 μg/mL and 400 μg/mL respective to extract fractionsX4andX5.

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Consolo, Verónica Fabiana, Cecilia Inés Mónaco, Cristina Alicia Cordo, and Graciela Lidia Salerno. "Characterization of novel Trichoderma spp. isolates as a search for effective biocontrollers of fungal diseases of economically important crops in Argentina." World Journal of Microbiology and Biotechnology 28, no. 4 (November 17, 2011): 1389–98. http://dx.doi.org/10.1007/s11274-011-0938-5.

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Rodhe, Henning. "Biocontrolled thermostasis involving the sulfur cycle." Climatic Change 8, no. 1 (February 1986): 91–92. http://dx.doi.org/10.1007/bf00158971.

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Ijntema, K., W. J. M. Heuvelsland, C. A. M. C. Dirix, and A. P. Sam. "Hydroxyapatite microcarriers for biocontrolled release of protein drugs." International Journal of Pharmaceutics 112, no. 3 (December 1994): 215–24. http://dx.doi.org/10.1016/0378-5173(94)90357-3.

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Cardona, Nadya L., Nicolás D. Franco-Sierra, and Javier Correa Alvarez. "Complete mitogenome of the biocontroller fungus Purpureocillium sp. (Ascomycota, Ophiocordycipitaceae, Hypocreales)." Mitochondrial DNA Part B 3, no. 2 (July 3, 2018): 1158–60. http://dx.doi.org/10.1080/23802359.2018.1522982.

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Schöbitz, Renate, Carla González, Katherine Villarreal, Mariela Horzella, Yanina Nahuelquín, and Ricardo Fuentes. "A biocontroller to eliminate Listeria monocytogenes from the food processing environment." Food Control 36, no. 1 (February 2014): 217–23. http://dx.doi.org/10.1016/j.foodcont.2013.08.017.

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Oporto, Christian, Carlos Villarroel, Sebastián Tapia, Verónica García, and Francisco Cubillos. "Distinct Transcriptional Changes in Response to Patulin Underlie Toxin Biosorption Differences in Saccharomyces cerevisiae." Toxins 11, no. 7 (July 10, 2019): 400. http://dx.doi.org/10.3390/toxins11070400.

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Patulin (4-hydroxy-4H-furo[3,2c]pyran-2[6H]-one) is a mycotoxin produced by a suite of fungi species. Patulin is toxic to humans and is a sporadic contaminant in products that were made from fungi-infected fruits. The baker yeast Saccharomyces cerevisiae (S. cerevisiae) has been shown to decrease patulin levels likely by converting it to the less harmful E-ascladiol, yet this capacity is dependent on the strain utilized. In this study we show that four representative strains of different S. cerevisiae lineages differ in their ability to tolerate and decrease patulin levels in solution, demonstrating that some strains are better suitable for patulin biocontrol. Indeed, we tested the biocontrol capacities of the best patulin-reducer strain (WE) in contaminated apple juice and demonstrated their potential role as an efficient natural biocontrol solution. To investigate the mechanisms behind the differences between strains, we explored transcriptomic changes of the top (WE strain) and worst (WA strain) patulin-biocontroller strains after being exposed to this toxin. Large and significant gene expression differences were found between these two strains, the majority of which represented genes associated with protein biosynthesis, cell wall composition and redox homeostasis. Interestingly, the WE isolate exhibited an overrepresentation of up-regulated genes involved in membrane components, suggesting an active role of the membrane towards patulin detoxification. In contrast, WA upregulated genes were associated with RNA metabolism and ribosome biogenesis, suggesting a patulin impact upon transcription and translation activity. These results suggest that different genotypes of S. cerevisiae encounter different stresses from patulin toxicity and that different rates of detoxification of this toxin might be related with the plasma membrane composition. Altogether, our data demonstrates the different molecular mechanisms in S. cerevisiae strains withstanding patulin exposure and opens new avenues for the selection of new patulin biocontroller strains.

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Prieto, L. M., G. R. Ochoa, E. Garcés de Granada, and F. Valcarcel. "SUSCEPTIBILITY OF FIVE DENDRANTHEMA GRANDIFLORA CULTIVARS TO PHOMA SP. AND EVALUATION OF A BIOCONTROLLER." Acta Horticulturae, no. 482 (March 1999): 219–22. http://dx.doi.org/10.17660/actahortic.1999.482.32.

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Zanatta, Zarela G. C. N., Andréa B. Moura, Luciano C. Maia, and André S. dos Santos. "Bioassay for selection of biocontroller bacteria against bean common blight (Xanthomonas axonopodis pv. phaseoli)." Brazilian Journal of Microbiology 38, no. 3 (September 2007): 511–15. http://dx.doi.org/10.1590/s1517-83822007000300024.

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Pons, Sophie, Saskia Bindschedler, David Sebag, Pilar Junier, Eric Verrecchia, and Guillaume Cailleau. "Biocontrolled soil nutrient distribution under the influence of an oxalogenic-oxalotrophic ecosystem." Plant and Soil 425, no. 1-2 (January 30, 2018): 145–60. http://dx.doi.org/10.1007/s11104-018-3573-1.

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Gajera, H. P., Darshna G. Hirpara, Disha D. Savaliya, and B. A. Golakiya. "Extracellular metabolomics of Trichoderma biocontroller for antifungal action to restrain Rhizoctonia solani Kuhn in cotton." Physiological and Molecular Plant Pathology 112 (December 2020): 101547. http://dx.doi.org/10.1016/j.pmpp.2020.101547.

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Frederic, Jean B., Cândido A. da Costa, Fernando da S. Rocha, and Maria de Fátima G. Fernandes. "Application forms and mode of action of biocontroller in the management of Meloidogyne incognita in tomato." Horticultura Brasileira 38, no. 3 (September 2020): 254–60. http://dx.doi.org/10.1590/s0102-053620200303.

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ABSTRACT Mixture of antagonist agents and form of application may influence in the control of root-knot nematodes. Thus, the aim of this work was to study the action of a biological product based on enzyme mixtures, Bacillus sp. and Trichoderma sp., at different concentrations on hatching, motility, mortality and reproduction of Meloidogyne incognita in tomato, considering two ways of application of the product. Eggs and second-stage juveniles (J2) of M. incognita were placed in biological product solutions at concentrations: 0, 1.25, 2.5, 5, 10, 20 and 40 g L-1. The same concentrations were applied to planting pits or to the soil surface. Afterwards, 3,692 eggs of M. incognita were inoculated in tomato crop. A significant reduction in J2 hatching of M. incognita J2 was observed in the highest concentrations and higher mortality of J2 from concentration of 5 g L-1. The interaction between concentrations and form of application of the product significantly influenced the infectivity and reproduction of M. incognita. Greater root system mass was obtained by applying the biological product to the surface, regardless of concentration.

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Mencarelli, Mariangela, Cesare Accinelli, and Alberto Vicari. "Implications of European corn borer, Ostrinia nubilalis , infestation in an Aspergillus flavus -biocontrolled corn agroecosystem." Pest Management Science 69, no. 9 (February 21, 2013): 1085–91. http://dx.doi.org/10.1002/ps.3472.

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Busto, Eduardo, Nina Richter, Barbara Grischek, and Wolfgang Kroutil. "Biocontrolled Formal Inversion or Retention ofL-α-Amino Acids to Enantiopure (R)- or (S)-Hydroxyacids." Chemistry - A European Journal 20, no. 35 (July 22, 2014): 11225–28. http://dx.doi.org/10.1002/chem.201403195.

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Kuchen, Benjamín, Yolanda Paola Maturano, María Victoria Mestre, Mariana Combina, María Eugenia Toro, and Fabio Vazquez. "Selection of Native Non-Saccharomyces Yeasts with Biocontrol Activity against Spoilage Yeasts in Order to Produce Healthy Regional Wines." Fermentation 5, no. 3 (July 8, 2019): 60. http://dx.doi.org/10.3390/fermentation5030060.

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Two major spoilage yeasts in the wine industry, Brettanomyces bruxellensis and Zygosaccharomyces rouxii, produce off-flavors and gas, causing considerable economic losses. Traditionally, SO2 has been used in winemaking to prevent spoilage, but strict regulations are in place regarding its use due to its toxic and allergenic effects. To reduce its usage researchers have been searching for alternative techniques. One alternative is biocontrol, which can be used either independently or in a complementary way to chemical control (SO2). The present study analyzed 122 native non-Saccharomyces yeasts for their biocontrol activity and their ability to be employed under fermentation conditions, as well as certain enological traits. After the native non-Saccharomyces yeasts were assayed for their biocontrol activity, 10 biocontroller yeasts were selected and assayed for their ability to prevail in the fermentation medium, as well as with respect to their corresponding positive/negative contribution to the wine. Two yeasts that satisfy these characteristics were Wickerhamomyces anomalus BWa156 and Metschnikowia pulcherrima BMp29, which were selected for further research in application to mixed fermentations.

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Sirini, Noelí, Paula Loyeau, María Ruiz, María Stegmayer, Lorena Soto, María Werning, Laureano Frizzo, Vanesa Ordoñez, Juana Fernández-López, and Marcelo Rosmini. "Development of Probiotic Fermented Sausages and Viability Monitoring of Supplemented Lactiplantibacillus plantarum BFL Strain." Fermentation 8, no. 10 (October 9, 2022): 526. http://dx.doi.org/10.3390/fermentation8100526.

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The reformulation of meat products is a pending task for the scientific-technological sector. Fermented meat products can carry probiotics, and studying their effect during the product shelf life currently represents a large area of vacancy. The objective of this work was to study the viability of microencapsulated (E) and unencapsulated (P) Lactiplantibacillus plantarum BFL as well as their effects on the microbiological and physicochemical parameters of fermented sausages preserved at 20 °C and 5 °C during 60 days of storage. The inoculated sausages (P and E) had significantly reduced pH values and potential pathogenic microorganism counts. The viability of encapsulated L. plantarum BFL (E) did not decline during storage as it did in its unencapsulated state (P). In addition, L. plantarum BFL could present an antioxidant effect at 20 °C towards the end of storage. The probiotic L. plantarum BFL generally tolerated the meat matrix conditions; it could be used as a biocontroller since its high viability rates would allow it to be projected as an adjunct culture for the meat industry. However, spray-drying microencapsulation of the probiotic L. plantarum BFL is not recommended as a viability-enhancing strategy in the Salamines Criollos studied in this work.

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Lorenzi, Adriana Sturion, Maria Letícia Bonatelli, Mathias Ahii Chia, Leonardo Peressim, and Maria Carolina Quecine. "Opposite Sides of Pantoea agglomerans and Its Associated Commercial Outlook." Microorganisms 10, no. 10 (October 20, 2022): 2072. http://dx.doi.org/10.3390/microorganisms10102072.

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Multifaceted microorganisms such as the bacterium Pantoea colonize a wide range of habitats and can exhibit both beneficial and harmful behaviors, which provide new insights into microbial ecology. In the agricultural context, several strains of Pantoea spp. can promote plant growth through direct or indirect mechanisms. Members of this genus contribute to plant growth mainly by increasing the supply of nitrogen, solubilizing ammonia and inorganic phosphate, and producing phytohormones (e.g., auxins). Several other studies have shown the potential of strains of Pantoea spp. to induce systemic resistance and protection against pests and pathogenic microorganisms in cultivated plants. Strains of the species Pantoea agglomerans deserve attention as a pest and phytopathogen control agent. Several of them also possess a biotechnological potential for therapeutic purposes (e.g., immunomodulators) and are implicated in human infections. Thus, the differentiation between the harmful and beneficial strains of P. agglomerans is mandatory to apply this bacterium safely as a biofertilizer or biocontroller. This review specifically evaluates the potential of the strain-associated features of P. agglomerans for bioprospecting and agricultural applications through its biological versatility as well as clarifying its potential animal and human health risks from a genomic point of view.

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Molina-Mercader, Gloria, Andrés O. Angulo, Eugenio Sanfuentes, Rodrigo Hasbún, Tania Olivares, Miguel Castillo-Salazar, and Claudio Goycoolea. "Detection and distribution of Ophelimus migdanorum and its possible biocontroller Closterocerus chamaeleon in productive areas of Eucalyptus globulus in Chile." Chilean journal of agricultural research 79, no. 3 (September 2019): 337–46. http://dx.doi.org/10.4067/s0718-58392019000300337.

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Peña, Rubén, Jeniffer Vílches, Camila G.-Poblete, and María Angélica Ganga. "Effect of Candida intermedia LAMAP1790 Antimicrobial Peptides against Wine-Spoilage Yeasts Brettanomyces bruxellensis and Pichia guilliermondii." Fermentation 6, no. 3 (July 1, 2020): 65. http://dx.doi.org/10.3390/fermentation6030065.

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Wine spoilage yeasts are one of the main issues in the winemaking industry, and the control of the Brettanomyces and Pichia genus is an important goal to reduce economic loses from undesired aromatic profiles. Previous studies have demonstrated that Candida intermedia LAMAP1790 produces antimicrobial peptides of molecular mass under 10 kDa with fungicide activity against Brettanomyces bruxellensis, without affecting the yeast Saccharomyces cerevisiae. So far, it has not been determined whether these peptides show biocontroller effect in this yeast or other spoilage yeasts, such as Pichia guilliermondii. In this work, we determined that the exposure of B. bruxellensis to the low-mass peptides contained in the culture supernatant of C. intermedia LAMAP1790 produces a continuous rise of reactive oxygen species (ROS) in this yeast, without presenting a significant effect on membrane damage. These observations can give an approach to the antifungal mechanism. In addition, we described a fungicide activity of these peptides fraction against two strains of P. guilliermondii in a laboratory medium. However, carrying out assays on synthetic must, peptides must show an effect on the growth of B. bruxellensis. Moreover, these results can be considered as a start to develop new strategies for the biocontrol of spoilage yeast.

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Harba, M., M. Jawhar, and M. I. E. Arabi. "In Vitro Antagonistic Activity of Diverse Bacillus Species Against Fusarium culmorum and F. solani Pathogens." Open Agriculture Journal 14, no. 1 (September 22, 2020): 157–63. http://dx.doi.org/10.2174/1874331502014010157.

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Background: Fusarium culmorum and Fusarium solani are economically important fungal pathogens of many plant species causing significant yield losses worldwide. Frequent uses of fungicides are hazardous to humans and the environment. Therefore, in vitro antagonistic activity of diverse Bacillus species isolates with biological potential activity to control these both pathogens should be investigated. Objective: The objectives were to isolate and identify the Bacillus spp., which are potential controls of F. culmorum and F. solani, and to characterize molecularly, at the species level, those isolates that have potential as biocontroller of the pathogens. Methods: The in vitro antagonistic potential of 40 Bacillus isolates against F. culmorum and F. solani was evaluated on the basis of fungal growth inhibition on nutrient broth culture. The colony morphology and the 16S rRNA gene sequencing of Bacillus spp. were used to identify the isolates. Results: Bacillus sp. isolates were identified as B. atrophaeus, B. subtilis, Paenibacillus polymxa, B. amyloliquefaciens, B. simplex and B. tequilensis. They had significant (P<0.05) antagonistic activities against F. culmorum and F. solani isolates as compared to the untreated control. The antagonistic effects varied depending on the Fusarium sp. The bacterial B. subtilis isolates SY116C and SY SY118C provided the most noteworthy result as both strongly inhibited mycelial growth of F. solani by 97.2%, while the B. tequilensis isolate SY145D was the most effective in the formation of inhibition zones against F. culmorum by 75%. Conclusion: It is apparent that Bacillus sp. isolates play an important role in the inhibition of growth of F. culmorum and F. solani, and that the B. subtilis isolates SY116C and SY118C had the highest biological potential activity against these fungi. These antagonistic effects may be important contributors as a biocontrol approach that could be employed as a part of integrated soil pathogen management system.

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Rini, Adityawati Fajar, Munti Yuhana, and Aris Tri Wahyudi. "Potency of sponge-associated bacteria producing bioactive compounds as biological control of vibriosis on shrimp." Jurnal Akuakultur Indonesia 16, no. 1 (July 5, 2017): 41. http://dx.doi.org/10.19027/jai.16.1.41-50.

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ABSTRACT Vibrio sp. is a pathogen that causes a decrease in white shrimp production. Sponge-associated bacteria are known as bioactive compounds producer in marine habitat, such as antibacteria, which can be an alternative solution in preventing vibriosis. Thus, this study was aimed to obtain a sponge-associated bacteria biocontroller to inhibit vibriosis in vitro, in vivo and also in 16S-rRNA, Non-ribosomal peptide synthase (NRPS) and polyketide synthase (PKS) genes to demonstrate its ability of synthesizing (bioactive). Aaptos sp. and Hyrtios sp. sponges were collected from Pramuka Island, Jakarta. The isolation using sea water complete (SWC) and zobel marine agar (ZMA) resulted in 174 isolates, and a total of 69 isolates were successfully screened based on their antibacterial compound activity, while a total of 47 isolates were observed to have negative hemolysis (in hemolytic test). The pathogenicity test used twelve selected isolates, that have broad spectrums of antibacteria activity and hemolysis negative, showed that the selected isolates were not pathogenic to post-larva shrimp, indicating that shrimp survival rate (pathogenicity test) did not significantly differ (P>0,05) compared to the negative control. Genetic analysis based on 16S-rRNA revealed three genera groups that belonged to Pseudomonas, Staphylococcus, and Alcaligenes. NRPS and PKS genes detection using PCR engendered four potential bacteria isolates, which have the NRPS gene only, and one isolate having the PKS gene only and one isolate having both NRPS and PKS genes, proving that the bacteria produce bioactive compounds. Keywords: NRPS, PKS, anti-vibriosis, white shrimp ABSTRAK Vibrio sp. merupakan salah satu bakteri patogen yang menyebabkan penurunan produksi udang. Bakteri yang berasosiasi dengan spons diketahui merupakan produsen senyawa bioaktif perairan salah satunya sebagai antibakteri. Hal ini dapat menjadi alternatif solusi dalam penanggulangan vibriosis. Penelitian ini bertujuan untuk memperoleh isolat bakteri asosiasi spons yang mempunyai kemampuan dalam menghambat vibriosis secara in vitro, in vivo dan mendeteksi gen 16S-rRNA, nonribosomal peptide synthase (NRPS) serta polyketide synthase (PKS) untuk memastikan kemampuan mensintesis senyawa bioaktif. Spons Aaptos sp. dan Hyrtios sp. berhasil dikoleksi dari perairan Pulau Pramuka, Kep. Seribu Jakarta. Isolasi bakteri dengan menggunakan media sea water complete (SWC) dan zobel marine agar (ZMA) diperoleh 174 isolat. Sebanyak 69 isolat terdeteksi memiliki aktivitas antibakteri. Uji hemolisis menunjukkan 47 isolat adalah hemolisis negatif. Uji patogenisitas menggunakan 12 isolat terpilih yang memiliki spektrum luas dan hemolisis negatif. Hasil uji patogenisitas tehadap 12 isolat menunjukkan bahwa semua isolat tidak bersifat patogen terhadap pascalarva udang vaname. Hal ini dibuktikan dengan sintasan pascalarva udang vaname yang tidak berbeda nyata (P>0,05) dengan kontrol negatif. Hasil uji tantang terhadap Vibrio harveyi diketahui sintasan pascalarva udang vaname (70±5,0–90±0,0%) memiliki perbedaan yang signifikan jika dibandingkan dengan kontrol positif (38,3±2,9%). Berdasarkan analisis sekuen gen 16S-rRNA, menunjukkan bahwa isolat-isolat tersebut memiliki kemiripan dengan genus Pseudomonas, Staphylococcus dan Alcaligenes. Deteksi gen NRPS dan PKS menggunakan PCR diperoleh empat isolat bakteri memiliki hanya gen NRPS, satu isolat memiliki hanya gen PKS, dan satu isolat memiliki kedua gen NRPS-PKS. Hal ini membuktikan bahwa keenam isolat memiliki potensi sebagai penghasil senyawa bioaktif. Kata kunci: NRPS, PKS, antivibriosis, udang vaname

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Gortari, Fermin, Maximo Ivan Petruk Nowosad, Margarita Esther Laczeski, Andrea Onetto, Iliana Julieta Cortese, Maria Lorena Castrillo, Gustavo Angel Bich, et al. "BIOFERTILIZERS AND BIOCONTROLLERS AS AN ALTERNATIVE TO THE USE OF CHEMICAL FERTILIZERS AND FUNGICIDES IN THE PROPAGATION OF YERBA MATE BY MINI-CUTTINGS." Revista Árvore 43, no. 4 (2019). http://dx.doi.org/10.1590/1806-90882019000400012.

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ABSTRACT The production of yerba mate seedlings through seeds has several limitations, which can be overcome by ex vitro vegetative propagation techniques such as the mini-cuttings, in which it is usually necessary to use synthetic chemical fertilizers and fungicides. However, there is a tendency towards sustainable agriculture, using biofertilizers (growth-promoting bacteria) and biocontrollers (Trichoderma sp.). Therefore, the objectives of this work were to evaluate the effect of biofertilizers on the production of mini-cuttings from yerba mate mini-stumps; as well as the effect, of biocontrollers on survival and rooting capacity of mini-cuttings. Strains of Bacillus sp. and Trichoderma asperelloides of yerba mate were used under two radiation conditions. There was a positive relationship between the availability of radiation and the production of mini-cuttings and the rooting capacity. All the mini-stumps sprouted regardless of treatments. The largest production of viable mini-cuttings occurred in a situation of high radiation and fertilization; while the treatments with growth-promoting bacteria and high radiation had intermediate values. The mini-cuttings inoculated with Trichoderma asperelloides had higher rooting percentage, greater number and length of roots than the mini-cuttings treated with fungicide. Therefore, we demonstrated that the use of chemical products can be replaced by biological ones and achieves acceptable yields.

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Godínez-Paoli, Rafael, Santos Gerardo Leyva-Mir, María Del Rosario García-Mateos, Jesús Magdaleno-Villar, Oscar Cruz-Álvarez, and María Teresa Martínez-Damián. "Fungicides, biocontrollers and resistance inducers in commercial bell pepper hybrids inoculated with Phytophthora capsici." Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology 38, no. 2 (April 9, 2020). http://dx.doi.org/10.18781/r.mex.fit.2002-1.

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El objetivo de esta investigación fue evaluar el nivel de resistencia de diferentes híbridos comerciales de pimiento morrón (Caoba rojo, Dicaprio amarillo, Pimiento Olvera, Sympathy, California wonder y PS16364212), a P. capsici mediante el uso de fungicidas, biocontroladores e inductores de resistencia. El ensayo se realizó bajo condiciones de invernadero en bolsas de polietileno negro con arena de tezontle como sustrato. Los productos se aplicaron de acuerdo con la dosis recomendada por el fabricante, aplicándose a los 10 y 20 días después del trasplante. Posteriormente, las plantas se inocularon con una solución de zoosporas móviles a una concentración de 1x10-6. Se realizaron tres evaluaciones visuales para el registro de incidencia, severidad y ABCPE. Se encontró que PS16364212 presentó mejor resistencia al ataque de P. capsici con 14.9% de incidencia y 15.7% dia-1 (ABCPE) respecto a California wonder (44.2% de incidencia). La aplicación de Metalaxil-M y Acibenzolar-S-metil mostraron la menor ABCPE (0.8 y 6.8% dia-1, respectivamente), por lo que ambos productos pueden ser considerados como una alternativa para su empleo dentro del manejo integrado de la marchitez del chile.

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Silva, Izael Oliveira, Edna Peixoto da Rocha Amorim, Nelson Augusto Nascimento Junior, Georgia Souza Peixinho, Juliana Paiva Carnaúba, and Vicente Ferreira de Araujo Neto. "Trichoderma spp antagonism to phytopathogens causing cassava root rot in alagoas." Revista Científica Multidisciplinar Núcleo do Conhecimento, July 7, 2020, 149–77. http://dx.doi.org/10.32749/nucleodoconhecimento.com.br/agronomy-en/trichoderma-antagonism.

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Several are phytopathogens that limit the production of cassava (Manihot esculata Crants) being rot of the roots to which they marry the greatest loss in the crop reaching 70%. This culture is of high interest to the Northeast region of Brazil, providing great losses. The difficulties in management have directed the search for alternatives, among which, biocontrollers have shown significant results. This literature review aimed to gather information related to the rot of cassava root cv Rosinha, the main phytopathogens and antagonistic activities of Trichoderma in the control of diseases in the state of Alagoas. A bibliographic survey was carried out in the databases Scielo, Google Acadêmico, Capes portal (theses and dissertations), journals and books. To obtain information on: root rot of table cassava, Trichoderma spp., as a biocontrol agent of phytopathogens.

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Ocegueda-Reyes, Martha Delia, Josefina Casas-Solís, Diego R. González-Eguiarte, and Gil Virgen-Calleros. "Rhizobacteria antagonistic to the fungus Sclerotium cepivorum (Berk.t) in onion plants (Allium cepa L.)." Journal of Enviromental Sciences and Natural Resources, December 31, 2019, 5–16. http://dx.doi.org/10.35429/jesn.2019.16.5.5.16.

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Rhizobacteria are microorganisms attached to the roots of plants that colonize the rhizosphere, there is a great diversity with beneficial effects on crop development and they used in biological control. In this study, the antagonistic effect of rhizobacteria as biocontrollers of Sclerotium cepivorum Berk fungus, which cause white onion rot in large economic losses, was determined. Soil samples collected from onion plants on the farm located in Santa Anita, municipality of Tlaquepaque, Jalisco; for isolation of rhizobacteria and S. cepivorum from infected white rot bulbs. Rhizobacteria that showed antagonism against the fungus, were selected 14 treatments formed with onion plants in conditions of humidity, temperature and photoperiod constant in growth chamber for 36 weeks. Growth promotion variables were evaluated and the data analyzed by ANOVA. Rhizobacteria showed an antagonistic effect against the fungus and stimulated fresh weight, root length, stem and bulb in onion plants. Therefore, this PGPR is an alternative as a biofertilizer potential, thus avoiding degradation and contamination of the soil and yield of product quality and profitability for the producer.

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Sootla, Aivar, Nicolas Delalez, Emmanouil Alexis, Arthur Norman, Harrison Steel, George H. Wadhams, and Antonis Papachristodoulou. "Dichotomous feedback: a signal sequestration-based feedback mechanism for biocontroller design." Journal of The Royal Society Interface 19, no. 189 (April 2022). http://dx.doi.org/10.1098/rsif.2021.0737.

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We introduce a new design framework for implementing negative feedback regulation in synthetic biology, which we term ‘dichotomous feedback’. Our approach is different from current methods, in that it sequesters existing fluxes in the process to be controlled, and in this way takes advantage of the process’s architecture to design the control law. This signal sequestration mechanism appears in many natural biological systems and can potentially be easier to realize than ‘molecular sequestration’ and other comparison motifs that are nowadays common in biomolecular feedback control design. The loop is closed by linking the strength of signal sequestration to the process output. Our feedback regulation mechanism is motivated by two-component signalling systems, where a second response regulator could be competing with the natural response regulator thus sequestering kinase activity. Here, dichotomous feedback is established by increasing the concentration of the second response regulator as the level of the output of the natural process increases. Extensive analysis demonstrates how this type of feedback shapes the signal response, attenuates intrinsic noise while increasing robustness and reducing crosstalk.

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Boada, Yadira, Fernando N. Santos-Navarro, Jesús Picó, and Alejandro Vignoni. "Modeling and Optimization of a Molecular Biocontroller for the Regulation of Complex Metabolic Pathways." Frontiers in Molecular Biosciences 9 (March 29, 2022). http://dx.doi.org/10.3389/fmolb.2022.801032.

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Achieving optimal production in microbial cell factories, robustness against changing intracellular and environmental perturbations requires the dynamic feedback regulation of the pathway of interest. Here, we consider a merging metabolic pathway motif, which appears in a wide range of metabolic engineering applications, including the production of phenylpropanoids among others. We present an approach to use a realistic model that accounts for in vivo implementation and then propose a methodology based on multiobjective optimization for the optimal tuning of the gene circuit parts composing the biomolecular controller and biosensor devices for a dynamic regulation strategy. We show how this approach can deal with the trade-offs between the performance of the regulated pathway, robustness to perturbations, and stability of the feedback loop. Using realistic models, our results suggest that the strategies for fine-tuning the trade-offs among performance, robustness, and stability in dynamic pathway regulation are complex. It is not always possible to infer them by simple inspection. This renders the use of the multiobjective optimization methodology valuable and necessary.

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Silva, Izael Oliveira, Edna Peixoto da Rocha Amorim, Nelson Augusto Nascimento Junior, Georgia Souza Peixinho, Juliana Paiva Carnaúba, and Vicente Ferreira de Araujo Neto. "Trichoderma spp Antagonismus zu Phytopathogenen verursacht Maniokwurzelfäule in Alagoas." Revista Científica Multidisciplinar Núcleo do Conhecimento, July 7, 2020, 149–77. http://dx.doi.org/10.32749/nucleodoconhecimento.com.br/agronomie-de/trichoderma-antagonismus.

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Einige sind Phytopathogene, die die Produktion von Maniava (Manihot esculata Crants) begrenzen, die Fäulnis der Wurzeln sind, mit denen sie den größten Verlust in der Ernte heiraten und 70% erreichen. Diese Kultur ist für die Region Nordostbrasilien von großem Interesse und verursacht große Verluste. Die Schwierigkeiten im Management haben die Suche nach Alternativen geleitet, unter denen Biocontroller signifikante Ergebnisse gezeigt haben. Diese Literaturrezension zielte darauf ab, Informationen über die Fäulnis der Maniokwurzel cv Rosinha, die wichtigsten Phytopathogene und antagonistische Aktivitäten der Trichodermie bei der Bekämpfung von Krankheiten im Bundesstaat Alagoas zu sammeln. Eine bibliographische Umfrage wurde in den Datenbanken Scielo, Google Acad’mico, Capes Portal (Thesen und Dissertationen), Zeitschriften und Büchern durchgeführt. Um Informationen über: Wurzelfäule von Tischmaniok, Trichoderma spp., als Biokontrollmittel von Phytopathogenen zu erhalten.

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Wang, Beibei, Hu Liu, Hailin Ma, Chengqiang Wang, Kai Liu, Yuhuan Li, Qihui Hou, et al. "Complete Genome Sequence of Biocontroller Bacillus velezensis Strain JTYP2, Isolated from Leaves of Echeveria laui." Genome Announcements 5, no. 24 (June 15, 2017). http://dx.doi.org/10.1128/genomea.00505-17.

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ABSTRACT Bacillus velezensis JTYP2 was isolated from the leaves of Echeveria laui in Qingzhou, China, and may control some of the fungal pathogens of the plant. Here, we present the complete genome sequence of B. velezensis JTYP2. Several gene clusters related to its biosynthesis of antimicrobial compounds were predicted.

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Silva, Izael Oliveira, Edna Peixoto da Rocha Amorim, Nelson Augusto Nascimento Junior, Georgia Souza Peixinho, Juliana Paiva Carnaúba, and Vicente Ferreira de Araujo Neto. "Antagonismus von Trichoderma spp. ai fitopatogeni che causano marciume della radice di manioca in Alagoas." Revista Científica Multidisciplinar Núcleo do Conhecimento, July 7, 2020, 149–77. http://dx.doi.org/10.32749/nucleodoconhecimento.com.br/agronomia-it/antagonismo-di-trichoderma.

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Molti sono fitopatogeni che limitano la produzione di manioca (Manihot esculata Crants) rotei delle radici a cui sposano la più grande perdita nel raccolto raggiungendo il 70%. Questa cultura è di grande interesse per la regione nord-orientale del Brasile, fornendo grandi perdite. Le difficoltà di gestione hanno diretto la ricerca di alternative, tra le quali i biocontrollori hanno mostrato risultati significativi. Questa revisione della letteratura mirava a raccogliere informazioni relative al marciume della radice di manioca cv Rosinha, i principali fitopatogeni e attività antagoniste del trichoderma nel controllo delle malattie nello stato di Alagoas. Un’indagine bibliografica è stata condotta nelle banche dati Scielo, Google Acad-mico, portale Capes (tesi e tesi), diari e libri. Per ottenere informazioni su: marciume radicale della cassavia tavolo, Trichoderma spp., come agente di biocontrollo dei fitopatogeni.

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Gavrilov, Sergey N., Daria G. Zavarzina, Ivan M. Elizarov, Tamara V. Tikhonova, Natalia I. Dergousova, Vladimir O. Popov, Jonathan R. Lloyd, et al. "Novel Extracellular Electron Transfer Channels in a Gram-Positive Thermophilic Bacterium." Frontiers in Microbiology 11 (January 11, 2021). http://dx.doi.org/10.3389/fmicb.2020.597818.

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Biogenic transformation of Fe minerals, associated with extracellular electron transfer (EET), allows microorganisms to exploit high-potential refractory electron acceptors for energy generation. EET-capable thermophiles are dominated by hyperthermophilic archaea and Gram-positive bacteria. Information on their EET pathways is sparse. Here, we describe EET channels in the thermophilic Gram-positive bacterium Carboxydothermus ferrireducens that drive exoelectrogenesis and rapid conversion of amorphous mineral ferrihydrite to large magnetite crystals. Microscopic studies indicated biocontrolled formation of unusual formicary-like ultrastructure of the magnetite crystals and revealed active colonization of anodes in bioelectrochemical systems (BESs) by C. ferrireducens. The internal structure of micron-scale biogenic magnetite crystals is reported for the first time. Genome analysis and expression profiling revealed three constitutive c-type multiheme cytochromes involved in electron exchange with ferrihydrite or an anode, sharing insignificant homology with previously described EET-related cytochromes thus representing novel determinants of EET. Our studies identify these cytochromes as extracellular and reveal potentially novel mechanisms of cell-to-mineral interactions in thermal environments.

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Castillo-Sanmiguel, Paula Andrea, Laura Rocío Cortés-Sánchez, and Jovanna Acero-Godoy. "Molecular aspects of tomato (Solanum lycopersicum) vascular wilt by Fusarium oxysporum f. sp. lycopersici and antagonism by Trichoderma spp." Revista Mexicana de Fitopatología, Mexican Journal of Phytopathology 40, no. 1 (December 15, 2021). http://dx.doi.org/10.18781/r.mex.fit.2106-1.

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Tomato plants (Solanum lycopersicum) are susceptible to the infection by diverse pathogens that cause devastating diseases such as vascular wilt, which causes great losses at the production level. The fungus Fusarium oxysporum f. sp. lycopersici (Fol) is one of the etiologic agents of this disease and its control lies in the use of synthetic chemicals which generate a negative impact in both health and the environment; thus, it is necessary to implement biological control as a healthier and more efficient alternative. The fungus Trichoderma spp. is a favorable option to be employed as a biocontroller against this pathogen thanks to its antagonist mechanisms, determined by metabolic and genetic characteristics. On the one hand, for Fol it is indispensable the activation of signaling routes such as MAPK Fmk1, MAPK Mpk1 y HOG, while Trichoderma spp. uses effectors involved in the interaction with the plant such as proteins, enzymes and secondary metabolites that also strengthen its immune response against infection, determined by both Pathogen Associated Molecular Patterns (PAMP) and effectors. Therefore, this article makes a review about the mentioned characteristics and suggests a greater application of tools and molecular markers for the management of this disease.

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Journal articles on the topic 'Biocontrollers'

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