Dissertations / Theses: 'Biocybernetic diagnostics and therapy'

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Smit, Jan Gerhard. "Grundlagen der wissenschaftlichen Biokybernetischen Diagnostik und Therapie für funktionell chronisch Kranke." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2010. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-62518.

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Das Buch enthält eine Reihe grundlegender Artikel zur Biokybernetischen Diagnostik und Therapie aus Vorträgen, Seminaren usw. Das Verfahren der ganzheitlichen „Biokybernetischen Diagnostik und Therapie“ geht weit über die Akupunktur hinaus. Sie ist keine Alternativmedizin, sondern für austherapierte funktionelle Kranke.

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Smit, Jan Gerhard. "Grundlagen der Kybernetischen Medizin (Reflexmedizin) mit Mikropressur und dem Reflexotron." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2010. http://nbn-resolving.de/urn:nbn:de:swb:14-opus-23441.

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Ehtaiba, Mabrouka Haiba. "Use of microwave techniques in medical diagnostics and therapy." Thesis, Cardiff University, 2017. http://orca.cf.ac.uk/103482/.

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Brennecke, Johannes. "Molecular diagnostics of the bacterial response to antibiotic therapy." Thesis, University of Edinburgh, 2017. http://hdl.handle.net/1842/28843.

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Bacterial bloodstream infections (BSIs) are a major healthcare problem causing high mortality and economic cost. BSIs require an immediate initiation of antibiotic therapy as any delay is associated with a mortality increase. With the emergence of antimicrobial resistance, the choice of the appropriate antibiotic becomes increasingly difficult, thus creating an urgent need for new diagnostics, ideally to be done at the point of care. The current gold standard is blood culture with subsequent susceptibility testing although several molecular methods have recently entered the market. However, in many instances there is a discrepancy between the in-vitro data provided by the test and the outcome of antimicrobial therapy in-vivo because current diagnostics fail to take into account the impact of the environment in the patient such as the immune system, pharmacokinetics and pharmacodynamics or bacterial fitness. In this thesis, it was hypothesised that the measurement of the bacterial gene expression after the beginning of antibiotic therapy might be a more accurate indicator of the therapy outcome because it reflects the bacterial response under in-vivo conditions. In the first part of the thesis the expression of a set of pre-defined mRNA markers was investigated under various conditions. Experiments conducted with clinical E. coli isolates incubated in human whole blood revealed an excellent correlation between the gene expression, the treatment outcome, the antibiotic susceptibility and the genetic background for three different classes of antimicrobial drugs. The second part of the thesis describes the extraction of bacterial RNA from human whole blood specimen. The effect of different agents for the lysis of human blood cells and the impact of co-purified human RNA were analysed and a method for high yield extraction of undegraded bacterial RNA was established. The third part of the thesis investigates two methods for the sensitive measurement of the bacterial gene expression. This is relevant because the bacterial loads in BSI patients are extremely low. For genes with high gene expression levels both methods yielded reliable results but were unable to quantify the expression of the previously investigated mRNA markers due to their low copy numbers. Other approaches, especially those based on single cell measurements, might be able to overcome the problem in the future and should be explored in greater detail. Overall, the foundations for a future diagnostic test based on the measurement of the bacterial gene expression have been laid in this work. Future work should address the mRNA quantification and further evaluate the connection between gene expression and therapy outcome, e.g. in animal models. A future diagnostic test should also fulfil point-of-care requirements. This will include integrated sample preparation and quantification as well as a time-to-result in the range of a few minutes.

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Conde, João Diogo Osório de Castro. "Cancer theranostics: multifunctional gold nanoparticles for diagnostics and therapy." Doctoral thesis, Faculdade de Ciências e Tecnologia, 2013. http://hdl.handle.net/10362/10927.

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Doctorate in Biology, Specialty in Biotechnology
The use of gold nanoparticles (AuNPs) has been gaining momentum in molecular diagnostics due to their unique physico-chemical properties these systems present huge advantages, such as increased sensitivity, reduced cost and potential for single-molecule characterisation. Because of their versatility and easy of functionalisation, multifunctional AuNPs have also been proposed as optimal delivery systems for therapy (nanovectors). Being able to produce such systems would mean the dawn of a new age in theranostics (diagnostics and therapy)driven by nanotechnology vehicles. Nanotechnology can be exploit for cancer theranostics via the development of diagnostics systems such as colorimetric and imunoassays, and in therapy approaches through gene therapy, drug delivery and tumour targeting systems. The unique characteristics of nanoparticles in the nanometre range, such as high surface-tovolume ratio or shape/size-dependent optical properties, are drastically different from those of their bulk materials and hold pledge in the clinical field for disease therapeutics This PhD project intends to optimise a gold-nanoparticle based technique for the detection of oncogenes’ transcripts (c-Myc and BCR-ABL) that can be used for the evaluation of the expression profile in cancer cells, while simultaneously developing an innovative platform of multifunctional gold nanoparticles (tumour markers, cell penetrating peptides, fluorescent dyes) loaded with siRNA capable of silencing the selected proto-oncogenes, which can be used to evaluate the level of expression and determine the efficiency of silencing. This work is a part of an ongoing collaboration between Research Centre for Human Molecular Genetics, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal and Biofunctional Nanoparticles and Surfaces Group, Instituto de Nanociencia de Aragón, Spain within a European project [NanoScieE+ - NANOTRUCK]. In order to achieve this goal we developed effective conjugation strategies to combine, in a highly controlled way, biomolecules to the surface of AuNPs with specific functions such as: ssDNA oligos to detect specific sequences and for mRNA quantification; Biofunctional spacers: Poly(ethylene glycol) (PEG) spacers used to increase solubility and biocompatibility and confer chemical functionality; Cell penetrating peptides: to overcome the lipophilic barrier of the cellular membranes and deliver molecules into cells using TAT peptide to achieve cytoplasm and nucleus; Quaternary ammonium: to introduce stable positively charged in gold nanoparticles surface; and RNA interference: siRNA complementary to a master regulator gene, the proto-oncogene c-Myc, that is implicated in cell growth, proliferation, loss of differentiation, and cell death. In order to establish that they are viable alternatives to the available methods, these innovative nanoparticles were extensively characterized on their chemical functionalization, ease of uptake, cellular toxicity and inflammation, and knockdown of MYC protein expression in several cancer cell lines and in in vivo models.
Fundação para a Ciência e Tecnologia - (SFRH/BD/62957/2009); PTDC/BIO/66514/2006; NANOLIGHT-PTDC/QUI-QUI/112597/2009; Silencing the silencers via multifunctional gold nanoconjugates towards cancer therapy - PTDC/BBB-NAN/1812/2012

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Hobson, N. J. "Nanoparticle theranostics for applications in cancer diagnostics and cancer therapy." Thesis, University College London (University of London), 2017. http://discovery.ucl.ac.uk/1546610/.

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Traditionally, medicine has been conducted using a diagnostic procedure followed by an appropriate therapy and monitored were possible. On the whole, these steps have happened independently of each other. In recent years however many have started to question this independent approach and have asked whether technologies that seek to combine diagnostics and therapies would be more beneficial at treating diseases. This new medical discipline has been termed theranostics. The aim of this project was to design and synthesise a novel theranostic nanoparticle, using a micelle forming amphiphilic carbohydrate, with the overall hypothesis of determining whether using a nanomedicine that can simultaneously image and treat would improve the effectiveness of a cancer treatment. Super paramagnetic iron oxide nanoparticles (IONPs) have gained considerable attention as an MRI contrast agent due to their unique magnetic properties and relatively inoffensive toxicity profile. Before IONPs may be used in a biological environment they must overcome several challenges, including being stable to aggregation and organ targeting. In this project a modified chitosan amphiphilic polymer was used to successfully formulate IONPs into colloidal stable aqueous dispersions using two different methods which produced blackberry nanoparticles and raspberry nanoparticles. The raspberry nanoparticles were extensively characterised in vitro and in vivo and were found to be highly effective as an MRI imaging probe for the liver and spleen. Following this, they were tested for their cancer imaging properties in an in vivo mouse tumour model. The drug loading capacity of the raspberry nanoparticles was investigated using lomustine, paclitaxel and methotrexate, however no effective drug encapsulation was determined in this project. Overall, a highly effective MRI probe was engineered and characterised, although its future success will be determined by its activity towards a disease target.

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Mohammad, Zadeh Maryam. "Towards high throughput mechanical testing of cells for diagnostics and therapy." Thesis, Heriot-Watt University, 2017. http://hdl.handle.net/10399/3345.

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This thesis will focus on the modelling and quantification of the mechanical properties of biological samples with the aid of Atomic Force Microscopy (AFM). The importance of studying the mechanical properties of biological samples lies in its potential use for both therapeutic and diagnostic research as well as in bioengineering fields such as designing/selecting appropriate cell sorting and separation techniques. First part of this thesis is focused on developing a mathematical model by considering the physical properties (size and membrane thickness) of cells, to measure the size changes and Young’s elastic modulus of mammalian cells. Developed model showed promising results in measuring the changes in the radius of the cells after deformation. Also, it has been showed that there is a direct relation between the shape of the indenter and obtained elastic modulus. Moreover, the Young’s elastic modulus derived from fitting the developed model into force-indentation curves was in the range of MPa. This is due to measuring the elastic properties of cells on the surface elements (lipid bilayer). Producing manufactured red blood cells (RBCs) is increasing due to the high demand for blood transfusions and lack of eligible donors. Manufactured RBCs require continuous sorting and separation process during their production, as well as at the end of the manufacturing process, where reticulocytes are formed. Selecting appropriate sorting and separation methods, which are highly efficient and have the minimum drawback, is essential. Techniques which are carry out the cell separation processes based on the mechanical and physical properties of cells shows promising results. Therefore, the second part of this thesis was designed to measure the mechanical and physical properties of CD34+ stem cells during their expansion and differentiation processes (Days 11, 14, 18 and 21) for cell purification and separation purposes. Obtained results showed that size-based techniques can be applied to sort cells at day 11 of differentiation process from the day 14, while for sorting day 14 cells from day 18 and day 18 cells from day 21, any elasticity or sized based techniques cannot provide a highly purified/sorted culture and significant contaminations would be remained. In addition, the mechanical properties of the extruded nucleus from the CD34+ cells was also quantified for studying their effects on the elastic properties of the CD34+ cells. Derived results showed that to eliminate the free-floating nucleus form the culture media, elasticity and size-based techniques can be used and provide a high efficient sorted culture (100% separation).

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Sandberg, Carin. "Aspects of fluorescence diagnostics and photodynamic therapy in non-melanoma skin cancer /." Göteborg : Department of Dermatology and Venereology, Sahlgrenska University Hospital, Institute of Clinical Sciences, Sahlgrenska Academy University, University of Gothenburg, 2009. http://hdl.handle.net/2077/21192.

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Persson, Mikael. "Antibody Mediated Radionuclide Targeting of HER-2 for Cancer Diagnostics and Therapy : Preclinical Studies." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ.-bibl. [distributör], 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-6798.

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Nestor, Marika. "Antibody-Based Radionuclide Targeting for Diagnostics and Therapy : Preclinical Studies on Head and Neck Cancer." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis, 2006. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-7341.

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Huang, Xiaohua. "Gold Nanoparticles Used in Cancer Cell Diagnostics, Selective Photothermal Therapy and Catalysis of NADH Oxidation Reaction." Diss., Georgia Institute of Technology, 2006. http://hdl.handle.net/1853/10565.

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Gold nanoparticles strongly absorb and scatter visible and near infrared light because of the strongly enhanced electric fields at the surface. This provides the potential of designing novel optically active reagents for simultaneous molecular imaging and photothermal cancer therapy. In this thesis, gold nanospheres and nanorods conjugated with anti-epidermal growth factor receptor (anti-EGFR) antibodies that specifically target EGFR on the cell surface are shown to be used for dual diagnostics and therapy. Using micro-absorption spectroscopy and light scattering imaging, cancerous (HOC 313 and HSC 3) and noncancerous cells (HaCat) can be differentiated due to the overexpression of EGFR on the surface of cancer cells. By irradiating the cells with a CW laser, selective photothermal cancer therapy is realized in visible region by using gold nanospheres and in near infrared region by using gold nanorods. The use of nanorods allow for in vivo therapy due to the fact that their absorption is in the near infrared region at which the laser light meets less interference from the tissue absorption. In addition, the catalytic effect of gold nanoparticles on the oxidization of NADH to NAD+ is investigated. The addition of gold nanoparticles is found to quench the NADH fluorescence intensities but has no effect on the fluorescence lifetime. This suggests that the fluorescence quenching is not due to coupling with the excited state, but due to changing the ground state of NADH. The intensity of the 340 nm absorption band of NADH is found to decrease while that of the 260 nm band of NAD+ is found to increase as the concentration of gold nanoparticles increase. This conversion reaction is further supported by nuclear magnetic resonance and mass spectroscopy. The linear relationship between the initial reaction rate of NADH and the concentration of gold nanoparticles strongly supports that NADH is surface catalyzed by the gold nanoparticles. The catalytic property of this important reaction might have important future applications in biological and medical fields.

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Schranz, Katrin [Verfasser], and Heinrich [Akademischer Betreuer] Leonhardt. "Investigation of AML-specific FLT3 mutations – from next generation diagnostics to combined targeted therapy / Katrin Schranz ; Betreuer: Heinrich Leonhardt." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1184202648/34.

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Whitney, Jon R. "Single Walled Carbon Nanohorns as Photothermal Absorbers, and Incorporation of Spatial Digital Image Analysis into Cancer Diagnostics and Therapy." Diss., Virginia Tech, 2013. http://hdl.handle.net/10919/50616.

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ï¿½Background: Photothermal therapy is an actively researched cancer treatment alternative to chemotherapy and resection due to its potential as a minimally invasive treatment with fewer health complications than high energy radiation therapies. ï¿½The effectiveness of photothermal therapy may be enhanced with the use of photoabsorbtive nanoparticles by increasing heat generation and improving spatial selectivity. ï¿½While photothermal therapy is a spatially distributed treatment, traditional experimental analysis methods used to assess photothermal therapy have either lacked spatial assessment such as is the case with standard viability assays of cell monolayers, or they only provide macroscopic treatment information, such as the measurement of the diameters of implanted mice flank tumors post-treatment. ï¿½
Goals: ï¿½This work aims to accomplish two major goals. ï¿½The first is to determine the therapeutic impact of combining Single Walled Carbon Nanohorns (SWNHs) with photothermal therapy. ï¿½The second is to advance the measurement tools used to assess photothermal therapy by developing viability measurement methods which incorporate detailed quantitative spatial information
Methods: Photothermal therapy was tested with and without SWNHs in in vitro cell monolayers, in vitro tissue phantoms, and ex-vivo tissue. ï¿½Digital image analysis methods were developed which allowed for the use of viability assays and histological information to be identified and organized spatially. ï¿½These methods were then used to compare the impact of cellular microenvironment and heating method on Arrhenius parameters.
Results: The inclusion of SWNHs dramatically increased the temperatures reached in each experiment. ï¿½Digital image analysis methods were shown to quantify spatial viability with a high degree of accuracy and precision in 2D and 3D. ï¿½Experimental data indicated that there were areas of collateral damage (partially treated tissue) surrounding areas of completely treated tissue ranging which were between 46% and 78% of the completely treated volume. ï¿½In each case the heat transfer properties of the experimental system had a large impact on the area of treatment. ï¿½Variation in the temperature and viability response of photothermal therapy for specific laser and nanoparticle treatment parameters was quantified. ï¿½
ï¿½ï¿½ï¿½ï¿½Conclusions: This research has brought an experimental cancer treatment procedure from experiments in cell monolayers to tests in ex-vivo tissue to analyze viability response. ï¿½The strengths of photothermal therapy such as its minimally invasive nature, and effectiveness at killing cells were experimentally demonstrated. ï¿½ ï¿½This research has also developed the tools necessary to assess the spatial impact in vitro and lay the foundations for assessing spatial impact in vivo. ï¿½These tools may be used to assess other treatments beyond photothermal therapy, and serve as a basis for improving the analysis of biological systems both in vitro and in vivo.

Ph. D.

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Bagley, Alexander Francis. "Optically-Active Nanomaterials for Diagnostic and Therapeutic Applications in Ovarian Cancer." Thesis, Harvard University, 2014. http://dissertations.umi.com/gsas.harvard:11517.

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The clinical management of cancer has principally relied upon surgery, radiation therapy, and chemotherapy for many decades. Despite recent advances in molecularly-targeted diagnostic and therapeutic agents, the long-term survival rates in patients with solid malignancies including ovarian cancer have improved only incrementally. Nanotechnologies designed to locally interrogate and modulate the tumor microenvironment offer a promising opportunity to enhance existing treatment modalities and establish new therapeutic paradigms. By virtue of their elemental composition, geometry, and surface chemistry, nanomaterials can be engineered with optical and pharmacokinetic properties which permit these agents to localize, fluoresce, and deposit energy within tumors. Nanomaterials therefore provide a clear route towards future approaches for sensitive diagnosis and imaging of tumors and targeted therapeutic delivery.

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Kranz, Jennifer, Stefanie Schmidt, Cordula Lebert, Laila Schneidewind, Falitsa Mandraka, Mirjam Kunze, Sina Helbig, et al. "The 2017 Update of the German Clinical Guideline on Epidemiology, Diagnostics, Therapy, Prevention, and Management of Uncomplicated Urinary Tract Infections in Adult Patients. Part II: Therapy and Prevention." Karger, 2018. https://tud.qucosa.de/id/qucosa%3A70643.

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Background: We aimed to update the 2010 evidence- and consensus-based national clinical guideline on the diagnosis and management of uncomplicated urinary tract infections (UTIs) in adult patients. Results are published in 2 parts. Part 1 covers methods, the definition of patient groups, and diagnostics. This second publication focuses on treatment of acute episodes of cystitis and pyelonephritis as well as on prophylaxis of recurrent UTIs. Materials and Methods: An interdisciplinary group consisting of 17 representatives of 12 medical societies and a patient representative was formed. Systematic literature searches were conducted in MEDLINE, EMBASE, and the Cochrane Library to identify literature published in 2010–2015. Results: For the treatment of acute uncomplicated cystitis (AUC), fosfomycin-trometamol, nitrofurantoin, nitroxoline, pivmecillinam, and trimethoprim (depending on the local rate of resistance) are all equally recommended. Cotrimoxazole, fluoroquinolones, and cephalosporins are not recommended as antibiotics of first choice, for concern of an unfavorable impact on the microbiome. Mild to moderate uncomplicated pyelonephritis should be treated with oral cefpodoxime, ceftibuten, ciprofloxacin, or levofloxacin. For AUC with mild to moderate symptoms, instead of antibiotics symptomatic treatment alone may be considered depending on patient preference after discussing adverse events and outcomes. Primarily non-antibiotic options are recommended for prophylaxis of recurrent urinary tract infection. Conclusion: In accordance with the global antibiotic stewardship initiative and considering new insights in scientific research, we updated our German clinical UTI guideline to promote a responsible antibiotic use and to give clear hands-on recommendations for the diagnosis and management of UTIs in adults in Germany for healthcare providers and patients.

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Spies, Jan-Peter [Verfasser], Alexander L. [Gutachter] Gerlach, and Charlotte [Gutachter] Hanisch. "PTSD in German Armed Forces service members – from diagnostics to therapy / Jan-Peter Spies ; Gutachter: Alexander L. Gerlach, Charlotte Hanisch." Köln : Universitäts- und Stadtbibliothek Köln, 2021. http://d-nb.info/1233426486/34.

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Kulyk, Olena. "Light-tissue interactions for developing portable and wearable optoelectronic devices for sensing of tissue condition, diagnostics and treatment in photodynamic therapy (PDT)." Thesis, University of St Andrews, 2016. http://hdl.handle.net/10023/13199.

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This thesis presents the development and in-vivo applications of wearable and portable devices for the investigation of light interaction with tissue involved in Photodynamic therapy (PDT) and during contraction of muscles. A hand-held device and a clinical method were developed for time course in-vivo imaging of the fluorescence of the photosensitizer Protoporphyrin IX (PpIX) in healthy and diseased skin with the aim to guide improvement of PDT protocols. The device was used in a small clinical study on 11 healthy volunteers and 13 patients diagnosed with non-melanoma skin cancer (NMSC). Two types of PpIX precursors were administered: Ameluz gel and Metvix® cream. The fluorescence was imaged with a 10 minute time step over three hours which was the recommended metabolism time before commencing PDT treatment at Ninewells Hospital, Dundee. The fluorescence time course was calculated by integrating the areas with the highest intensity. The fluorescence continued to grow in all subjects during the three hours. The time course varied between individuals. There was no statistical significance between either healthy volunteers or patients in Ameluz vs Metvix® groups; nor was there statistical difference between the three lesions groups (Actinic keratosis (AK) Ameluz vs AK Metvix® vs Basal cell carcinoma (BCC) Metvix®). The p-value was larger than 0.05 in a two sample t-test with unequal variances for all the groups. However, there was strong body site dependence between the head & neck compared to the lower leg & feet, or the trunk & hands body site groups (p-value < 0.01). One of the possible explanations for this was temperature and vasculature variation in skin at different body sites: the temperature is higher and the vasculature structure is denser at the head and the neck compared to the lower leg or the trunk. The temperature was not measured during the study. So in order to support this hypothesis, typical skin temperatures at the lesion sites were taken from the IR thermal images of healthy skin available in literature. PpIX fluorescence had a positive correlation to temperature. If this hypothesis is true, it will be highly important to PDT treatment. Increasing the temperature could speed up the metabolism and reduce the waiting time before starting the treatment; ambient temperature should be taken into account for daylight PDT; cooling air as pain management should be administered with caution. Potential improvements for wearable PDT light sources were investigated by modelling light transport in skin for the current LED-based Ambulight PDT device, a commercial OLED for future devices and a directional OLED developed in the group. The optical models were implemented in commercial optical software (with intrinsic Monte Carlo ray tracing and Henyey-Greenstein scattering approximation) which was validated on diffuse reflectance and transmittance measurements using in-house made tissue phantoms. The modelling was applied to investigate the benefits from diffusive and forward scattering properties of skin on light transmission in treatment light sources. 1 mm thick skin can only compensate approximately 10% of non-uniform irradiance. It means that uniform illumination is crucial for the treatment light sources. Forward scattering in skin showed a 10% improved light transmission from a collimated emission compared to a wide angle Lambertian emission. However, depth-dependent transmission measurements of directional vs Lambertian emission from organic light emitting films (a nano-imprinted grating was fabricated to provide directional emission in one of the films), collimated vs diffused HeNe laser light through fresh porcine skin did not show the expected improvement. This could be explained by skin roughness which was previously found to change the optical properties and may also affect light coupling. The modelling was applied to guide an optical design of another wearable device – a muscle contraction sensor. Muscle is fibrous and because of that scatters light differently in different directions. The sensor detects the change in backscattered light in parallel and perpendicular directions with respect to muscle fibres. The sensor was implemented on a wearable bandage on fully flexible substrate with flexible OLED and organic photodiodes. The major advantages of organic optoelectronic sensing compared to conventional electromyography (EMG) sensors are the ability to distinguish two types of contractions (isotonic and isometric), insensitivity to electromagnetic interference and the absence of an immune response due to non-invasive electrode-free sensing. Optical modelling was performed to understand the operation of the sensor. A 3D anisotropic optical model of scattering in muscle was created by geometrical manipulations with the standard Henyey-Greenstein scattering volumes. The penetration depth from the Super Yellow OLED was found to be 20-25 mm; the optimal separation between the source and the detector was found to be 20 mm. This distance provided a still detectable signal along with the best discrimination between the two backscatterings. When a 2 mm thick layer of skin and a 2 mm thick layer of adipose tissue were added to the model, the signal was hugely diffused. The discrimination between the two backscatterings decreased by three orders of magnitude, the penetration depth in muscle was reduced, and the intensity of the signal dropped down but was still detectable. With 5 mm thick adipose tissue and 2 mm thick skin the signal was too diffused and interacted with very shallow layers of muscle which approached the limits of the optical sensing of muscle activity.

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Radziej, Katharina [Verfasser], Jürgen [Akademischer Betreuer] Beckmann, Anna [Gutachter] Buchheim, Jürgen [Gutachter] Beckmann, and Claas [Gutachter] Lahmann. "Psychopathological precursors and sequelae of functional vertigo and dizziness disorders : From diagnostics to therapy / Katharina Radziej ; Gutachter: Anna Buchheim, Jürgen Beckmann, Claas Lahmann ; Betreuer: Jürgen Beckmann." München : Universitätsbibliothek der TU München, 2019. http://d-nb.info/1187443883/34.

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Sundberg, Åsa Liljegren. "Tumour Targeting Using Radiolabelled EGF Conjugates : Preclinical Studies." Doctoral thesis, Uppsala University, Biomedical Radiation Sciences, 2004. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-4201.

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Tumour targeted radiotherapy is an appealing approach for treatment of disseminated tumour cells. A targeting agent that specifically binds to a structure on tumour cells is then used to transport therapeutically relevant radionuclides. The epidermal growth factor receptor, EGFR, is overexpressed on tumour cells in several malignancies, e.g. highly malignant gliomas. In this thesis, three types of radiolabelled EGF-conjugates, aimed for targeting to EGFR-expressing tumour cells, were developed and studied: EGF-dextran labelled with ¹²⁵I, EGF labelled with ²¹¹At, and two EGF-chelates, DTPA-EGF and Bz-DTPA-EGF, labelled with the radioactive metals ¹¹¹In and ¹⁷⁷Lu.

The targeting properties of radioiodinated EGF-dextran were first studied in cultured glioma cells. Radioiodine coupled to the dextran part of EGF-dextran was retained in cells appreciably longer than radioiodine coupled to EGF. This can give about 100 times increased radiation dose to tumour cells.

Targeting with ²¹¹At-EGF was investigated in combination with the tyrosine kinase inhibitor gefitinib (Iressa™, ZD1839). The uptake of ²¹¹At-EGF in EGFR-expressing tumour cells increased with increasing gefitinib concentrations. This was the case for both gefitinib-resistant and gefitinib-sensitive cell lines. The effect of the combined treatment on cell survival, however, differed between the cell lines in an unexpected way. In gefitinib resistant cells, combined treatment decreased cell survival approximately 3.5 times relative to ²¹¹At-EGF treatment alone. In gefitinib sensitive cells, however, combined treatment increased the cell survival (i.e. a protective effect).

The EGF-chelates studied ([¹¹¹In]DTPA-EGF, [¹¹¹In]Bz-DTPA-EGF and [¹⁷⁷Lu]Bz-DTPA-EGF) all bound specifically with high affinity (K_d≈2 nM) to EGFR on cultured glioma cells. They were internalised after binding, and the cellular retention of radionuclides was high (60% remained after 45 h). A biodistribution study in mice showed that liver and kidneys accumulated a majority of the radioactivity. The EGF-chelates bound EGFR specifically also in vivo. A tumour-to-blood ratio of 25 was achieved in a preliminary study.

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Alves, Renata Ramos. "Diadococinesia oral em crianças com e sem transtorno fonológico." Universidade de São Paulo, 2011. http://www.teses.usp.br/teses/disponiveis/8/8139/tde-07072011-130100/.

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A diadococinesia (DDK) oral é uma medida auxiliar para a produção dos sons da fala que avalia as habilidades motoras orais, fornecendo meios de avaliar a evolução da maturação e da integração neuromotora do indivíduo. Essa pesquisa teve como objetivo descrever o desempenho das crianças com e sem transtorno fonológico na prova de DDK, verificando a relação dessa prova com os processos fonológicos e o índice de gravidade PCC-R. Participaram do estudo 124 sujeitos, com idade entre 5 e 10:8 anos, sendo divididos em subgrupos em função do diagnóstico de TF e da idade: GC 5 a 7:11 anos; GC 8 a 10:08 anos; GP 5 a 7:11 anos e GP 8 a 10:08 anos. A DDK foi avaliada por meio da repetição das sequências Pa, Ta, Ka, Pataka, Aí, Aú e Iú, sendo calculados os índices sequência por segundo e porcentagem de erros. Independentemente da sequência testada não ocorreram diferenças significantes entre o gênero dos sujeitos. As crianças do GP apresentaram maior porcentagem média de erro na produção das sequências, no entanto tal resultado apresentou diferença estatisticamente significante apenas na sequência Pataka. Nas sequências Ta, Ka e Aú, independente de grupo ou gênero, crianças de 5 a 7:11 apresentaram média de repetição de sequência por segundo menor do que as crianças de 8 a 10:08. Na sequência Pataka essa progressão foi encontrada apenas no GC. As crianças do GP, quando comparadas com o GC, produziram menos sequências por segundo na sequência Pataka na faixa etária de 8 a 10:08 anos, sendo possível estabelecer um valor de corte para essa faixa etária: 1,4 sequência por segundo. Identificou-se, então, que os sujeitos abaixo do valor de corte apresentam menor PCC-R e produzem mais processos fonológicos de Ensurdecimento de Fricativa e Simplificação de Líquidas na prova de Imitação e Ensurdecimento de Plosiva na prova de Nomeação. Foi possível concluir que crianças com Transtorno Fonológico apresentam desempenho diferente em relação à produção de movimentos repetidos e alternados, sendo a sequência Pataka a que melhor identificou essa diferença. Assim esta prova pode ser um importante instrumento de auxílio no diagnóstico do TF.
The oral diadochokinesia (DDK) is a helpful measure for the production of speech sounds that evaluates the oral motor skills, providing a mean of assessing the development of neuromotor maturation and integration of the individual. This study aimed to describe the performance of children with and without phonological disorders in DDK tasks, and also verifying the relationship between phonological processes and the index PCC-R. The study included 124 subjects, aged 5 years and 10:8, divided into subgroups according to age and diagnosis: children without phonological disorders-Control Group (CG) 5 to 7:11 years, CG 8 to 10:08 years; children with phonological disorders-Research Group (RG) 5 to 7:11, RG 8 to 10:08. The DDK was assessed by repeating sequences of Pa, Ta, Ka, Pataka, Aí, Aú, Iú, and the rates were calculated in sequence per second and percentage of errors. The results pointed that, regardless of the sequence tested, no significant differences between the genders of the subjects were found. The children from the RG had higher average percentage of error in the sequences production, however, statistic difference was noted only in the sequence Pataka. In sequences Ta, Ka and Aú, regardless of group or gender, children of 5 to 7:11 years had a mean repetition of sequences per second lower than children of 8 to 10:08 years. In Pataka this progression was found only in CG. The children from the RG 8 to 10:08 years, compared with GC 8 to 10:08 years, produced less sequence per second in the sequence Pataka, so was possible to establish a cutoff value for this age group: 1,4 sequences per second. This value indicated that subjects below the cutoff have lower PCC-R and produce more phonological processes of Fricative Devoicing and Liquid Simplification in the Imitation test and Plosive Devoicing in the Naming test. It was concluded that children with phonological disorder exhibit different performance in relation to the production of repeated and alternated movements. The sequence that best identified this difference was Pataka, showing that this test may be an important tool for the diagnosis of TF.

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Short, Robert Franklin. "Novel approaches in imaging and image-guided therapy microfabrication, quantitative diagnostic methods, and a model of lymphangiogenesis /." Connect to resource, 2005. http://rave.ohiolink.edu/etdc/view?acc%5Fnum=osu1117553725.

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Thesis (Ph. D.)--Ohio State University, 2005.
Title from first page of PDF file. Document formatted into pages; contains xvi, 218 p.; also includes graphics (some col.). Includes bibliographical references (p. 200-218). Available online via OhioLINK's ETD Center

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22

Westphal, Maximillian. "Investigation of low energy, alternative X-ray sources and their interactions with multi-Z materials for theranostics." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1571133365330023.

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23

Kessler, Mariella Lena [Verfasser], Georg [Akademischer Betreuer] Berding, Peter [Akademischer Betreuer] Behrens, and Kirsten [Akademischer Betreuer] Müller-Vahl. "Improved understanding of the pathophysiology and therapy of hearing impairment based on functional and molecular diagnostics using emission tomography / Mariella Lena Kessler ; Akademische Betreuer: Georg Berding, Peter Behrens, Kirsten Müller-Vahl ; Hannover Biomedical Research School, Klinik für Nuklearmedizin." Hannover : Bibliothek der Medizinischen Hochschule Hannover, 2019. http://d-nb.info/1201612586/34.

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Kessler, Mariella [Verfasser], Georg [Akademischer Betreuer] Berding, Peter [Akademischer Betreuer] Behrens, and Kirsten [Akademischer Betreuer] Müller-Vahl. "Improved understanding of the pathophysiology and therapy of hearing impairment based on functional and molecular diagnostics using emission tomography / Mariella Lena Kessler ; Akademische Betreuer: Georg Berding, Peter Behrens, Kirsten Müller-Vahl ; Hannover Biomedical Research School, Klinik für Nuklearmedizin." Hannover : Bibliothek der Medizinischen Hochschule Hannover, 2019. http://d-nb.info/1201612586/34.

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25

Wittchen, Hans Ulrich. "Klinische Psychologie und Verhaltenstherapie - zwischen Aufstieg und Erosion." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2013. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-91232.

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Der Beitrag diskutiert Probleme der raschen Weiterentwicklung von Klinischer Psychologie und der Verhaltenstherapie im besonderen. Dabei werden drei Perspektiven angesprochen: (a) Binnenbeziehungen innerhalb des Fachs Klinische Psychologie sowie zu Nachbardisziplinen, (b) Transferprobleme wissenschaftlicher Erkenntnisse von der Forschung zur Praxis und (c) Probleme der Fort-und Weiterbildung sowie der Qualitätssicherung in der Verhaltenstherapie. Als Beispiele von Fortschritt und Erosion werden diskutiert: (a) die Verhaltensmedizin, als Muster für gut abgestimmte und in die Klinische Psychologie als Fach integrierte Entwicklung, (b) die Gesundheitspsychologie für eine schlechte Interaktionskultur mit mangelhaftem gegenseitigem Informationstransfer und (c) die Psychotherapieszene als Beispiel für Erosionsprozesse in Forschung, Praxis sowie vor allem rort-und Weiterbildung. Der Beitrag fordert eine wesentliche Stärkung des Fachs Klinische Psychologie als fachliche und organisatorische Klammer zwischen den auseinanderdriftenden Entwicklungen. Eine erfolgreiche Übernahme dieser universitär verankerten Koordinations-und Integrations aufgabe erfordert allerdings gleichzeitig auch eine erhebliche Ausweitung personeller Ressourcen und fachlicher Kompetenzen. Eine zentrale neue Herausforderung für klinisch-psychologische Universitätsinstitute besteht auch in der Entwicklung von Qualitätssicherungsmaßnahmen. Der Beitrag empfiehlt in diesem Zusammenhang, vor allem in der Fort-und Weiterbildung den verstärkten Einsatz von Therapiemanualen sowie die lnstitutionalisierung von regelmäßigen Konsensuskonferenzen mit Empfehlungen zur Therapiedurchführung
This paper discusses progress and erosion aspects of c1inical psychology and behavior therapy in Germany from three interrelated perspectives: (a) the relationship of behavior therapy and c1inical psychology to other basic and applied psychological disciplines as weIl as neighboring disciplines, (b) the transfer problems from the scientific fields to practice, and (c) the problem of quality assurance in practice and postgraduate education. Specific emphasis is laid on a discussion of the field of behavioral medicine, as an example for well-integrated and coordinated research and practice activities; health psychology as an example for deficient communication patterns with clinical psychology and behavior therapy, and psychotherapy as an example for erosion in research, education and practice. The paper strongly recommends a more dominant steering role of clinical psychology as the most comprehensive scientific discipline. This steering role, however, would also require a considerably expanded infrastructure of clinical psychology departments in universities together with several mechanisms (competence enhancement, consensus conferences, development of postgraduate education guidelines, quality assurance activities, coordination) to be able to fulfill this mission. The paper also suggests the more frequent use of standardized treatment manuals in postgraduate courses

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Kunert, Christoph. "Entwicklung eines Multi-Leaf Faraday Cups zur Strahldiagnose in der Augentumortherapie." Doctoral thesis, Humboldt-Universität zu Berlin, Mathematisch-Naturwissenschaftliche Fakultät, 2015. http://dx.doi.org/10.18452/17153.

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Die Protonentherapie von Aderhautmelanomen wird vor allem für die Behandlung von Tumoren nahe kritischer Strukturen (Sehnerv) und bei großen Tumoren angewandt. Dabei ist die begrenzte Reichweite der Protonen vorteilhaft, die scharf begrenzte Dosisfelder im Auge ermöglicht, und das an den Tumor grenzende gesunde Gewebe bestmöglich schont. Daher erfolgt die Positionierung der Patienten und der Strahlenfelder in der Augentumortherapie, wie auch die regelmäßigen Konstanzprüfungen, mit einer Reichweitengenauigkeit in Wasser von 0,1 mm. Mit einem Multi-Leaf Faraday Cup (MLFC) kann die Reichweite der Protonen in kurzer Zeit sehr genau gemessen werden. Dabei misst der MLFC die differentielle Fluenz der Protonenstrahlen, also das Reichweitenprofil. Er besteht aus einem Stapel Folien, abwechselnd leitend und isolierend. Eindringende Protonen deponieren eine zusätzliche Ladung in der Folie in der sie stoppen. Durch eine gleichzeitige Strommessung an allen Folien misst der MLFC relativ schnell die Reichweite der Protonen. Aufgabe dieser Arbeit ist es, einen MLFC entsprechend den Anforderungen der Augentumortherapie zu entwickeln, aufzubauen und mögliche Anwendungspotentiale zu untersuchen. Dafür wurden Monte-Carlo-Rechnungen mit MCNPX 2.6 und SRIM durchgeführt, verschiedene Folienstapel an Luft und im Vakuum untersucht, verschiedene Messelektroniken zur gleichzeitigen Messung von Strömen im pA-Bereich in vielen Kanälen getestet, ein Absorbersystem für einen variablen Messbereich von 30 MeV bis 70 MeV aufgebaut und die entsprechende Mess- und Steuersoftware in LabVIEW 2011 entwickelt. Es wurde die Genauigkeit der Reichweitenmessungen untersucht und gezeigt, dass der MLFC durch seine Mobilität eine schnelle Energiebestimmung an unterschiedlichen Experimentierplätzen erlaubt. In der Therapie ist neben der einfachen Bestimmung der maximalen Reichweite der Protonen auch die regelmäßige Kontrolle der Modulation der ausgedehnten Bragg-Kurven möglich.
Proton therapy of uveal melanomas is primarily used for the treatment of tumors near critical structures (optic nerve) and in large tumors. The great advantage of protons is their sharply limited range in tissue, which leads to sharp defined dose fields in the eye and the dose absorbed by the healthy tissue around the tumor can be reduced. Therefore, the positioning of the patient and the radiation fields, as well as the regular control measurements in the eye tumor therapy requires an accuracy of 0.1 mm in water. A Multi-Leaf Faraday Cup (MLFC) gives the opportunity to measure the proton range relatively fast and accurate. The MLFC measures the differential fluence, which means the range profile of the proton beam. It consists of a stack of sheets, alternating conductive and insulating, and the penetrating protons bring their additional charge into the sheet in which they stop. By measuring the corresponding current in each conducting sheet at the same time, the MLFC can quickly measure the range of the protons. The task of this work is to develop a MLFC with respect to the requirements of the eye tumor therapy and to explore possible application potentials. Therefore, Monte Carlo calculations with MCNPX 2.6 and SRIM were conducted, various foil stacks were studied in air and in vacuum, different measurement electronics for measuring currents in the pA range in many channels simultaneously were tested, a system of degraders for a variable measuring range from 30 MeV to 70 MeV was developed and the corresponding measurement and control software was written in LabVIEW 2011. The accuracy of the range measurements was examined and it was shown that a quick energy measurement at different target stations can be made by the MLFC due to its mobility. In therapy, in addition to the determination of the maximum range of the proton beam, the regular monitoring of the modulation of the extended Bragg-curves is in principle possible.

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Wittchen, Hans Ulrich. "Klinische Psychologie und Verhaltenstherapie - zwischen Aufstieg und Erosion." Karger, 1996. https://tud.qucosa.de/id/qucosa%3A26090.

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Der Beitrag diskutiert Probleme der raschen Weiterentwicklung von Klinischer Psychologie und der Verhaltenstherapie im besonderen. Dabei werden drei Perspektiven angesprochen: (a) Binnenbeziehungen innerhalb des Fachs Klinische Psychologie sowie zu Nachbardisziplinen, (b) Transferprobleme wissenschaftlicher Erkenntnisse von der Forschung zur Praxis und (c) Probleme der Fort-und Weiterbildung sowie der Qualitätssicherung in der Verhaltenstherapie. Als Beispiele von Fortschritt und Erosion werden diskutiert: (a) die Verhaltensmedizin, als Muster für gut abgestimmte und in die Klinische Psychologie als Fach integrierte Entwicklung, (b) die Gesundheitspsychologie für eine schlechte Interaktionskultur mit mangelhaftem gegenseitigem Informationstransfer und (c) die Psychotherapieszene als Beispiel für Erosionsprozesse in Forschung, Praxis sowie vor allem rort-und Weiterbildung. Der Beitrag fordert eine wesentliche Stärkung des Fachs Klinische Psychologie als fachliche und organisatorische Klammer zwischen den auseinanderdriftenden Entwicklungen. Eine erfolgreiche Übernahme dieser universitär verankerten Koordinations-und Integrations aufgabe erfordert allerdings gleichzeitig auch eine erhebliche Ausweitung personeller Ressourcen und fachlicher Kompetenzen. Eine zentrale neue Herausforderung für klinisch-psychologische Universitätsinstitute besteht auch in der Entwicklung von Qualitätssicherungsmaßnahmen. Der Beitrag empfiehlt in diesem Zusammenhang, vor allem in der Fort-und Weiterbildung den verstärkten Einsatz von Therapiemanualen sowie die lnstitutionalisierung von regelmäßigen Konsensuskonferenzen mit Empfehlungen zur Therapiedurchführung.
This paper discusses progress and erosion aspects of c1inical psychology and behavior therapy in Germany from three interrelated perspectives: (a) the relationship of behavior therapy and c1inical psychology to other basic and applied psychological disciplines as weIl as neighboring disciplines, (b) the transfer problems from the scientific fields to practice, and (c) the problem of quality assurance in practice and postgraduate education. Specific emphasis is laid on a discussion of the field of behavioral medicine, as an example for well-integrated and coordinated research and practice activities; health psychology as an example for deficient communication patterns with clinical psychology and behavior therapy, and psychotherapy as an example for erosion in research, education and practice. The paper strongly recommends a more dominant steering role of clinical psychology as the most comprehensive scientific discipline. This steering role, however, would also require a considerably expanded infrastructure of clinical psychology departments in universities together with several mechanisms (competence enhancement, consensus conferences, development of postgraduate education guidelines, quality assurance activities, coordination) to be able to fulfill this mission. The paper also suggests the more frequent use of standardized treatment manuals in postgraduate courses.

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28

Wittchen, Hans-Ulrich. "Klinische Psychologie und Verhaltenstherapie - zwischen Aufstieg und Erosion." Saechsische Landesbibliothek- Staats- und Universitaetsbibliothek Dresden, 2012. http://nbn-resolving.de/urn:nbn:de:bsz:14-qucosa-99894.

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Der Beitrag diskutiert Probleme der raschen Weiterentwicklung von Klinischer Psychologie und der Verhaltenstherapie im besonderen. Dabei werden drei Perspektiven angesprochen: (a) Binnenbeziehungen innerhalb des Fachs Klinische Psychologie sowie zu Nachbardisziplinen, (b) Transferprobleme wissen-schaftlicher Erkenntnisse von der Forschung zur Praxis und (c) Probleme der Fort– und Weiterbildung sowie der Qualitätssicherung in der Verhaltenstherapie. Als Beispiele von Fortschritt und Erosion werden diskutiert: (a) die Verhaltensmedizin, als Muster für gut abgestimmte und in die Klinische Psychologie als Fach integrierte Entwicklung, (b) die Gesundheitspsychologie für eine schlechte Interaktionskultur mit mangelhaftem gegen-seitigem Informatiûnstransfer und (c) die Psychotherapieszene als Beispiel für Erosionsprozesse in Forschung, Praxis sowie vor allem Fort– und Weiterbildung. Der Beitrag fordert eine wesentliche Stärkung des Fachs Klinische Psychologie als fachliche und organisatorische Klammer zwischen den auseinanderdriftenden Entwicklungen. Eine erfolgreiche Übernahme dieser universitär verankerten Koordinations- und Integrationsaufgabe erfordert allerdings gleichzeitig auch eine erhebliche Ausweitung personeller Ressourcen und fachlicher Kompetenzen. Eine zentrale neue Herausforderung für klinisch-psychologische Universitätsinstitute besteht auch in der Entwicklung von Qualitätssicherungsmaβnahmen. Der Beitrag empfiehlt in diesem Zusammenhang, vor allem in der Fort– und Weiterbildung den verstärkten Einsatz von Therapiemanualen sowie die Institutionalisierung von regelmäβigen Konsensuskonferenzen mit Empfehlungen zur Therapiedurchführung
This paper discusses progress and erosion aspects of c1inical psychology and behavior therapy in Germany from three interrelated perspectives: (a) the relationship of behavior therapy and c1inical psychology to other basic and applied psychological disciplines as weIl as neighboring disciplines, (b) the transfer problems from the scientific fields to practice, and (c) the problem of quality assurance in practice and postgraduate education. Specific emphasis is laid on a discussion of the field of behavioral medicine, as an example for well-integrated and coordinated research and practice activities; health psychology as an example for deficient communication patterns with clinical psychology and behavior therapy, and psychotherapy as an example for erosion in research, education and practice. The paper strongly recommends a more dominant steering role of clinical psychology as the most comprehensive scientific discipline. This steering role, however, would also require a considerably expanded infrastructure of clinical psychology departments in universities together with several mechanisms (competence enhancement, consensus conferences, development of postgraduate education guidelines, quality assurance activities, coordination) to be able to fulfill this mission. The paper also suggests the more frequent use of standardized treatment manuals in postgraduate courses

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29

Wittchen, Hans-Ulrich. "Klinische Psychologie und Verhaltenstherapie - zwischen Aufstieg und Erosion." Karger, 1996. https://tud.qucosa.de/id/qucosa%3A26265.

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Der Beitrag diskutiert Probleme der raschen Weiterentwicklung von Klinischer Psychologie und der Verhaltenstherapie im besonderen. Dabei werden drei Perspektiven angesprochen: (a) Binnenbeziehungen innerhalb des Fachs Klinische Psychologie sowie zu Nachbardisziplinen, (b) Transferprobleme wissen-schaftlicher Erkenntnisse von der Forschung zur Praxis und (c) Probleme der Fort– und Weiterbildung sowie der Qualitätssicherung in der Verhaltenstherapie. Als Beispiele von Fortschritt und Erosion werden diskutiert: (a) die Verhaltensmedizin, als Muster für gut abgestimmte und in die Klinische Psychologie als Fach integrierte Entwicklung, (b) die Gesundheitspsychologie für eine schlechte Interaktionskultur mit mangelhaftem gegen-seitigem Informatiûnstransfer und (c) die Psychotherapieszene als Beispiel für Erosionsprozesse in Forschung, Praxis sowie vor allem Fort– und Weiterbildung. Der Beitrag fordert eine wesentliche Stärkung des Fachs Klinische Psychologie als fachliche und organisatorische Klammer zwischen den auseinanderdriftenden Entwicklungen. Eine erfolgreiche Übernahme dieser universitär verankerten Koordinations- und Integrationsaufgabe erfordert allerdings gleichzeitig auch eine erhebliche Ausweitung personeller Ressourcen und fachlicher Kompetenzen. Eine zentrale neue Herausforderung für klinisch-psychologische Universitätsinstitute besteht auch in der Entwicklung von Qualitätssicherungsmaβnahmen. Der Beitrag empfiehlt in diesem Zusammenhang, vor allem in der Fort– und Weiterbildung den verstärkten Einsatz von Therapiemanualen sowie die Institutionalisierung von regelmäβigen Konsensuskonferenzen mit Empfehlungen zur Therapiedurchführung.
This paper discusses progress and erosion aspects of c1inical psychology and behavior therapy in Germany from three interrelated perspectives: (a) the relationship of behavior therapy and c1inical psychology to other basic and applied psychological disciplines as weIl as neighboring disciplines, (b) the transfer problems from the scientific fields to practice, and (c) the problem of quality assurance in practice and postgraduate education. Specific emphasis is laid on a discussion of the field of behavioral medicine, as an example for well-integrated and coordinated research and practice activities; health psychology as an example for deficient communication patterns with clinical psychology and behavior therapy, and psychotherapy as an example for erosion in research, education and practice. The paper strongly recommends a more dominant steering role of clinical psychology as the most comprehensive scientific discipline. This steering role, however, would also require a considerably expanded infrastructure of clinical psychology departments in universities together with several mechanisms (competence enhancement, consensus conferences, development of postgraduate education guidelines, quality assurance activities, coordination) to be able to fulfill this mission. The paper also suggests the more frequent use of standardized treatment manuals in postgraduate courses.

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30

Chin, Lee. "Optical diagnostics toolbox for laser interstitial thermal therapy." 2007. http://link.library.utoronto.ca/eir/EIRdetail.cfm?Resources__ID=742431&T=F.

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Fales, Andrew. "Development of Plasmonic Nanoplatforms for Diagnostics, Therapy, and Sensing." Diss., 2016. http://hdl.handle.net/10161/12871.

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Recent advances in nanotechnology have led to the application of nanoparticles in a wide variety of fields. In the field of nanomedicine, there is great emphasis on combining diagnostic and therapeutic modalities into a single nanoparticle construct (theranostics). In particular, anisotropic nanoparticles have shown great potential for surface-enhanced Raman scattering (SERS) detection due to their unique optical properties. Gold nanostars are a type of anisotropic nanoparticle with one of the highest SERS enhancement factors in a non-aggregated state. By utilizing the distinct characteristics of gold nanostars, new plasmonic materials for diagnostics, therapy, and sensing can be synthesized. The work described herein is divided into two main themes. The first half presents a novel, theranostic nanoplatform that can be used for both SERS detection and photodynamic therapy (PDT). The second half involves the rational design of silver-coated gold nanostars for increasing SERS signal intensity and improving reproducibility and quantification in SERS measurements.

The theranostic nanoplatforms consist of Raman-labeled gold nanostars coated with a silica shell. Photosensitizer molecules for PDT can be loaded into the silica matrix, while retaining the SERS signal of the gold nanostar core. SERS detection and PDT are performed at different wavelengths, so there is no interference between the diagnostic and therapeutic modalities. Singlet oxygen generation (a measure of PDT effectiveness) was demonstrated from the drug-loaded nanocomposites. In vitro testing with breast cancer cells showed that the nanoplatform could be successfully used for PDT. When further conjugating the nanoplatform with a cell-penetrating peptide (CPP), efficacy of both SERS detection and PDT is enhanced.

The rational design of plasmonic nanoparticles for SERS sensing involved the synthesis of silver-coated gold nanostars. Investigation of the silver coating process revealed that preservation of the gold nanostar tips was necessary to achieve the increased SERS intensity. At the optimal amount of silver coating, the SERS intensity is increased by over an order of magnitude. It was determined that a majority of the increased SERS signal can be attributed to reducing the inner filter effect, as the silver coating process moves the extinction of the particles far away from the laser excitation line. To improve reproducibility and quantitative SERS detection, an internal standard was incorporated into the particles. By embedding a small-molecule dye between the gold and silver surfaces, SERS signal was obtained both from the internal dye and external analyte on the particle surface. By normalizing the external analyte signal to the internal reference signal, reproducibility and quantitative analysis are improved in a variety of experimental conditions.

Dissertation

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32

Brückmann, Nadine E. [Verfasser]. "Polymer-metallodrug conjugates for cancer diagnostics and therapy / submitted by Nadine E. Brückmann." 2010. http://d-nb.info/1012883612/34.

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Amendoeira, Ana Filipa Patrício. "Steroid-BODIPY theranostics: Receptor-mediated cell uptake and applications in cancer Diagnostics and therapy." Master's thesis, 2020. http://hdl.handle.net/10362/103277.

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Cancer is still one of the leading cause of death worldwide despite the intensive investigation of the disease mechanisms and the development of new therapeutic approaches. Therefore, it is urgent to develop novel diagnostic and treatment platforms more selective and sparing healthy cells and overcoming resistance of cancer cells. Here, we report the study of estradiol-, testosterone- and nortestosterone derivates conjugated to BODIPY dye as a potential multi-modality theranostic agents (positron emission tomography (PET)/ fluorescence and photodynamic therapy (PDT)). Cellular uptake assays were performed in relevant breast and prostate cancer cells, as well as in normal fibroblasts, using fluorescence microscopy, in order to evaluate the trafficking pathways. Results showed a non-specific internalization of conjugates in cancer and normal cells, suggesting an energy-dependent cellular uptake through caveolae-mediated endocytosis. 2D co-cultures demonstrated that the conjugates are more specific for cancer cells. Cell viability assays showed that BODIPY conjugates are non-toxic for cancer and normal cells. In contrast, upon visible light irradiation a severe cell death effect was observed. Results demonstrated that EE2-C8 and HA-4198 steroid-BODIPY conjugates are potential photosensitizers for PDT against breast and prostate cancer cells. Future work are needed to gain more clues into the mechanism of action induced by these platforms after PDT.

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Maňáková, Kateřina. "Návrh terapeutických materiálů pro klienty s diagnózou afázie." Master's thesis, 2015. http://www.nusl.cz/ntk/nusl-335381.

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The thesis with the topic of proposing new therapeutic materials for clients diagnosed with aphasia brings more profound understanding of separate field of speech therapy, which is aphasiology. The first part of the thesis introduces the theoretical basis to the issues of aphasia, concentrates on fundamental definition of aphasia, etiology and symptomatology. Moreover, the thesis describes the possibilities of aphasia therapy and lastly, it covers the differential diagnostics of aphasia. The second part of the thesis covers in detail the Boston Classification System of Aphasia, introduces to the reader the foundations that were the basis for the propositions of the therapeutic materials. The second part describes in detail the very process of designing therapeutic materials in general and it also provides concrete propositions of such materials with possible applications. The appendix includes examples of proposed materials, both theoretically explained and physically created for the reader to examine.

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"Leveraging Artificial Intelligence to Find Previously Undiscovered Patterns in Tumor Molecular Data to Aid in Diagnosis and Therapy Selection." Doctoral diss., 2020. http://hdl.handle.net/2286/R.I.62903.

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abstract: Cancer researchers have traditionally used a handful of markers to understand the origin of tumors and to predict therapeutic response. Additionally, performing machine learning activities on disparate data sources of varying quality is fraught with inherent bias. The Caris Life Sciences Molecular Database (CMD) is an immense resource for discovery as it contains over 215,000 molecular profiles of tumors with consistently gathered clinical grade molecular data along with immense amounts of clinical outcomes data. This resource was leveraged to generate two artificial intelligence algorithms aiding in diagnosis and one for therapy selection. The Molecular Disease Classifier (MDC) was trained on 34,352 cases and tested on 15,473 unambiguously diagnosed cases. The MDC predicted the correct tumor type out of thirteen possibilities in the labeled data set with sensitivity, specificity, PPV, and NPV of 90.5%, 99.2%, 90.5% and 99.2% respectively when considering up to 5 predictions for a case. The availability of whole transcriptome data in the CMD prompted its inclusion into a new platform called MI GPSai (MI Genomic Prevalence Score). The algorithm trained on genomic data from 34,352 cases and genomic and transcriptomic data from 23,137 cases and was validated on 19,555 cases. MI GPSai can predict the correct tumor type out of 21 possibilities on 93% of cases with 94% accuracy. When considering the top two predictions for a case, the accuracy increases to 97%. Finally, a 67 gene molecular signature predictive of efficacy of oxaliplatin-based chemotherapy in patients with metastatic colorectal cancer was developed - FOLFOXai. The signature was predictive of survival in an independent real-world evidence (RWE) dataset of 412 patients who had received FOLFOX/BV in 1st line and inversely predictive of survival in RWE data from 55 patients who had received 1st line FOLFIRI. Blinded analysis of TRIBE2 samples confirmed that FOLFOXai was predictive of OS in both oxaliplatin-containing arms (FOLFOX HR=0.629, p=0.04 and FOLFOXIRI HR=0.483, p=0.02).
Dissertation/Thesis
Doctoral Dissertation Molecular and Cellular Biology 2020

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Leite, João Maria Silva Pinto. "Multifunctional cellulose nanoparticles for potential application on the diagnosis and treatment of cancer." Master's thesis, 2021. http://hdl.handle.net/10773/30892.

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Biopolymeric nanobeads stand out as being biocompatible, biodegradable, and chemically versatile nanomaterials for several biomedical applications. In this context, cellulose has shown an increasing potential in this field due to its abundance of hydroxyl groups and consequent ability to be functionalized. Therefore, the objective of the present dissertation consists in the preparation and characterization of multifunctional cellulose-based nanobeads for potential application in cancer diagnosis and treatment. Spherical cellulose-based nanobeads were produced by nanoprecipitation and functionalized with gold nanoparticles (AuNPs) that will play the dual role of cell imaging and therapeutic agent. The cellulose nanobeads were prepared using cellulose acetate (CA) as the starting raw material instead of directly using cellulose because of its high insolubility in most common solvents. So, CA nanobeads were obtained by nanoprecipitation through CA dissolution and regeneration, followed by alkaline hydrolysis to obtain the cellulose nanobeads. Subsequently, cellulose nanobeads/AuNPs hybrid systems were developed by two distinct methodologies, namely by CA regeneration in the presence of AuNPs and subsequent alkaline hydrolysis, or by the in situ reduction of the gold salt in the presence of the cellulose nanobeads. The cellulose nanobeads/AuNPs hybrids were then characterized by scanning electron microscopy (SEM), scanning transmission electron microscopy (STEM), attenuated total reflection-Fourier transform infrared (ATR-FTIR) spectroscopy, and ultraviolet-visible spectroscopy (UV-vis). Overall, the production of the cellulose nanobeads was achieved by dissolving CA in acetone and subsequent regeneration using water as the non-solvent, followed by alkaline hydrolysis. The success of the hydrolysis was confirmed by ATR-FTIR spectroscopy and SEM, while the synthesis of AuNPs was corroborated by UV-vis spectroscopy and STEM. The morphology and size of the hybrid systems were evaluated by SEM and STEM, which confirmed the production of the cellulose nanobeads/AuNPs hybrids with an average size of 415±187 nm for the cellulose nanobeads and 15±3 nm for the AuNPs. Moreover, the in vitro cytotoxicity of the cellulose nanobeads/AuNPs hybrids towards the pigmented human melanoma (MNT-1) cell line was evaluated for 24 h. The resultant data showed that the hybrid system exhibits a dose dependent cellular toxicity, reaching 81.6±4.5% of cell viability for 39.0 μg mL–1 of hybrid system. Thus, a higher dose will most definitely translate into a higher cytotoxic effect towards the tumor cells. All the obtained results revealed that the cellulose nanobeads/AuNPs hybrids have potential for application in the diagnosis and therapy of cancer.
Nanobeads biopoliméricas destacam-se como sendo nanomateriais biocompatíveis, biodegradáveis e quimicamente versáteis para diversas aplicações biomédicas. Neste âmbito, a celulose tem demonstrado um potencial crescente para estas aplicações devido à sua abundância de grupos hidroxilo e consequente aptidão para ser funcionalizada. Neste contexto, o objetivo da presente dissertação consiste na preparação e caracterização de nanobeads multifuncionais à base de celulose para potencial aplicação no diagnóstico e tratamento do cancro. Nanobeads esféricas à base de celulose foram produzidas por nanoprecipitação e funcionalizadas com nanopartículas de ouro (AuNPs) que podem desempenhar um papel duplo como agente de contraste para imagiologia e agente terapêutico. As nanobeads de celulose foram preparadas usando acetato de celulose (CA) como matéria-prima inicial em vez de usar diretamente a celulose devido à sua insolubilidade na maioria dos solventes convencionais. Deste modo, as nanobeads de CA foram obtidas por nanoprecipitação através da dissolução e regeneração do CA, seguida de uma hidrólise alcalina para a obtenção das nanobeads de celulose. Posteriormente, desenvolveram-se sistemas híbridos de nanobeads de celulose/AuNPs seguindo duas metodologias distintas, nomeadamente, por regeneração de CA na presença de AuNPs e subsequente hidrólise alcalina, ou pela redução in situ do sal de ouro na presença das nanobeads de celulose. Os sistemas híbridos foram posteriormente caracterizados por microscopia eletrónica de varrimento (SEM), microscopia eletrónica de varrimento em modo de transmissão (STEM), espectroscopia de infravermelho com reflexão total atenuada e transformada de Fourier (ATR-FTIR), e espectroscopia de ultravioleta-visível (UV-vis). Em suma, a produção de nanobeads de celulose foi conseguida dissolvendo o CA em acetona e subsequente regeneração usando água como o anti solvente, seguida de hidrólise alcalina. O sucesso da hidrólise foi comprovado por espectroscopia de ATR-FTIR e SEM, enquanto que a síntese das AuNPs foi confirmada por espectroscopia de UV-vis e STEM. A morfologia e o tamanho dos sistemas híbridos foram avaliados por SEM e STEM, os quais confirmaram a produção de sistemas híbridos de nanobeads de celulose/AuNPs com tamanho médio de 415±187 nm para as nanobeads de celulose e 15±3 nm para as AuNPs. Adicionalmente, a citotoxicidade in vitro dos sistemas híbridos foi avaliada em células humanas pigmentadas de melanoma (MNT-1) ao fim de 24 h. Os resultados mostraram que o sistema híbrido possui uma toxicidade celular dependente da concentração, alcançando 81.6±4.5% de viabilidade celular para 39.0 μg mL–1 do híbrido. Deste modo, uma concentração mais elevada promoverá uma maior resposta citotóxica nas células cancerígenas. Todos os resultados obtidos evidenciam o potencial dos sistemas híbridos de nanobeads de celulose/AuNPs para aplicação no diagnóstico e terapia do cancro.
Mestrado em Materiais e Dispositivos Biomédicos

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Malabi, Rudzani. "Laser-based technologies for targeted drug delivery and label-free diagnostics in HIV-1." Thesis, 2019. http://hdl.handle.net/10500/27259.

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Human immunodeficiency virus type 1 (HIV-1) still causes a chronic infection that affects millions of individuals worldwide. The infection remains incurable and presents a huge challenge for treatment, as it tends to disable a patient’s immune system. Although the current HIV-1 treatment regime possesses the ability to reduce the viral load to undetectable limits, complete eradication of the virus cannot be achieved while latent HIV-1 reservoirs go unchallenged. These viral reservoirs are established early on during HIV-1 infection and are a major hurdle since they remain unaffected by antiretroviral drugs and have the ability to replenish systemic infections once treatment is interrupted. Further ailments with the highly active antiretroviral therapy (HAART) include issues such as the cumbersome lifelong treatment, development of drug resistant strains of HIV-1 and adverse side effects. Contrarily, early diagnosis of the HIV-1 infection and HIV-1 treatment is a major challenge in resource-limited countries. The current available diagnostic tools for HIV-1 infection have shown to be highly accurate in monitoring CD4+ T lymphocyte count and viral load measurements. However, these tests such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR) which are highly efficient, are usually very expensive with complex operation, time consuming, require skilled personnel and training that makes them incompatible for the application in resource-limited areas. Therefore, this raises the urgent need for developing an HIV point of care (POC) diagnostic tool that is label-free, highly specific and sensitive as well as therapeutic modalities, which can be used to address the previously mentioned challenges. Much research has been conducted to resolve these problems but to date, there has not been application of laser and/or photonics in HIV research. Therefore, in this thesis a femtosecond laser was used in HIV infected cells for targeted antiretroviral drug delivery while preserving their viability. For the first time according to our knowledge, antiretrovirals (ARVs) that target all the life stages of the HIV-1 life cycle were utilized and they proved to be significant in reducing HIV-1 infection. Furthermore, through the employment of a continuous wave laser at 640 nm, for the first time, surface plasmon resonance was conducted to facilitate label-free detection of HIV-1. Success of these laser based technologies will open doors for incorporation in POC HIV diagnostic tools for the detection and treatment monitoring of HIV in resource-limited settings.
College of Engineering, Science and Technology
Ph. D. (Physics)

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Mahomed, Nasreen. "Diagnostic utility of chest X-rays in childhood community acquired pneumonia in the era of bacterial conjugate vaccines, antiretroviral therapy, molecular diagnostics and computer aided diagnosis." Thesis, 2017. http://hdl.handle.net/10539/23155.

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A thesis submitted to the Faculty of Health Sciences, University of Witwatersrand, Johannesburg, in fulfilment of the requirements for the Degree of Doctor of Philosophy in the branch of Medicine: Diagnostic Radiology Johannesburg, 2017.
Introduction: Pneumonia is among the leading infectious causes of morbidity and mortality in children under 5 years globally. The chest X-ray remains the most readily available and most common imaging modality for the assessment of childhood pneumonia. Chest X-ray quality assurance is important to maintain high image quality, allowing for more accurate diagnosis. Chest X-ray patterns in a high HIV prevalence setting in children hospitalized with pneumonia in the era of bacterial conjugate vaccines has not been described. Furthermore the association between chest X-ray findings and microbiological etiology using novel models is important. Standardization of chest X-ray interpretation is important to allow comparison of research findings between studies and has been proposed by the World Health Organisation (WHO) as a measure of bacterial vaccine efficacy (VE). The role of Computer Aided Diagnosis (CAD) for chest X-ray interpretation is important, especially in countries with limited radiologists. Objectives: In this thesis we investigated the quality of chest X-rays and the impact of quality assurance activities in the Pneumonia Etiology Research for Child Health (PERCH) study, from 9 sites in 7 countries (Bangladesh, the Gambia, Kenya, Mali, South Africa, Thailand and Zambia), by quantifying radiographic errors through use of a customized quality assessment form. Within the context of the PERCH South African site, we compared chest X-ray patterns in HIV-unexposed, HIV- exposed-uninfected (HEU) and HIV-infected children hospitalized with WHO-defined severe or very severe community acquired pneumonia. Further, we evaluated the association of chest X-ray patterns with clinical parameters and its association with the PERCH Quantitative Analysis (PQA) model predicted probability of infection by a class or specific pathogens. Lastly, we evaluated the utility of CAD for identifying chest X-ray primary end-point pneumonia (CXR-PEP) versus non CXR-PEP compared to a consensus human interpretation as a reference standard. Results: A total of 747 chest X-rays in 9 PERCH sites had chest X-ray quality assessment. Collimation performed the worse of the 6 parameters, with 5 out of the 9 sites being graded sub-optimally for this. Three of the 9 sites showed a significant improvement in chest X-ray quality using a test for trend analysis. At the South African PERCH site, 920 cases were enrolled over two years, including 858 children with interpretable chest X-rays. The commonest finding was CXR-PEP, prevalent among 38% HIV-unexposed children, 33% HEU children and 60% of HIV-infected children, which was consistent between different age categories. CXR-PEP was twice as common in HIV-infected (OR 2.5; 95% CI 1.6-3.8) compared with these HIV-unexposed children. Clinical and laboratory features independently associated with CXR-PEP included the presence of severe malnutrition, fever and CRP > 40mg/dL. CXR-PEP was associated with the composite outcome of mechanical ventilation or death. However there was no single clinical or laboratory parameter that had both high sensitivity and specificity to discriminate between CXR-PEP from non CXR-PEP cases. HIV-unexposed children with a PQA model probability index > 0.5 for bacterial etiology had 2-fold greater odds (95% CI 1.1-4.1) for CXR-PEP compared to non CXR-PEP, and similarly HIV-unexposed children with microbiologically confirmed pneumococcal pneumonia (MCPP) had 3-fold greater odds (95% CI 1.3-6.7) of CXR-PEP. HIV-infected children with a PQA model probability index > 0.5 for respiratory viral etiology and PQA model probability index > 0.5 for Pneumocystis jiroveci etiology trended to have a greater odds for CXR-PEP vs non CXR-PEP. Using CAD4WHOKids for the 858 interpretable chest X-rays, for CXR-PEP versus non CXR-PEP, CAD4WHOKids had a sensitivity of 76%, specificity of 80% and area under the ROC curve of 0.850 (95% CI 0.823-0.876) compared to the radiologist consensus reading. Conclusion: Chest X-ray quality, in particular collimation was suboptimal in 7 resource limited countries, in children hospitalized for WHO defined severe or very severe pneumonia. CXR-PEP remains the most common chest X-ray abnormality in HIV-unexposed, HEU and HIV-infected children under 5 years of age hospitalized for WHO-defined severe or very severe pneumonia, even in the era of routine HiB and PCV immunization. Our findings support the literature that CXR-PEP is of limited use in HIV-infected children as an outcome measure in bacterial VE studies, due to opportunistic infections like Pneumocystis jiroveci pneumonia and respiratory viral infections contributing to CXR-PEP. CAD is promising as a diagnostic tool for identifying WHO defined CXR-PEP in bacterial VE trials and pneumonia epidemiological studies.
MT2017

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Neugebauerová, Štěpánka. "Návrhy materiálů pro klienty klubu Afázie." Master's thesis, 2020. http://www.nusl.cz/ntk/nusl-412091.

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The diploma thesis focuses on the elaboration of proposals for materials for clients of the Aphasia Club, which were used in group therapy. The diploma thesis is divided into theoretical and practical part. The theoretical part contains three main chapters, in which the concept of aphasia is defined, the symptomatology and etiology of this imaired communication ability are described. Then are listed possible classifications of aphasia, available tests for diagnosis and directions in the therapy of aphasia. The practical parts sets the main goal of the thesis, together with three research questions. As research methods were chosen case studies, which were prepared on the basis of medical reports of individual clients of the Aphasia Club. With information of medical reports were created materials for group therapies of Aphasia Club. The materials were used in group therapies. Using method of observation, research questions focused on the use of materials, their modification and practical signifikance in the development of impaired communication functions were subsequently answered.

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Berenguer, Albiñana Carlos. "Příprava inhibitorů Neuraminidasy vhodných pro teranostiku." Doctoral thesis, 2018. http://www.nusl.cz/ntk/nusl-451117.

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Influenza viruses cause respiratory illnesses which can vary in severity depending on the strain of the virus, as well as the age and health condition of the host. Influenza remains a major threat to public health due to its nature prone to suffer mutations. As a result, vaccines have to be reformulated annually and new strains may cause sporadic global pandemics. Furthermore, the recent emergence of resistant strains of the virus against the current standard of care (oseltamivir and zanamivir) underlines the need of novel anti-influenza therapeutics. The aim of this dissertation work is to contribute to the discovery of new anti-influenza inhibitors either by rational drug-design and optimization of oseltamivir structure, or by developing screening assays suitable for the discovery of novel inhibitors of the enzymes neuraminidase or RNA-polymerase. Scheme 1. Overview of the strategy used for the development of new anti-influenza therapeutics. The dashed arrows indicate the inhibitors that were converted into probes and their corresponding target enzymes Two main modification points were explored for the improvement of oseltamivir properties (Scheme 1); modifications at carbon C-3 aimed to overcome oseltamivir resistance caused by common mutations like H274Y, meanwhile modifications at carbon C-5...

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Pluhařová, Apolena. "Laboratorní funkční diagnostika malabsorpčních syndromů se zaměřením na laktózovou intoleranci." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-447321.

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This diploma thesis deals with laboratory functional diagnostics of malabsorption syndromes. Especially the functional diagnosis of lactose intolerance. The golden standard for the functional diagnosis of lactose intolerance is the breath test. The aim of the study was to determine the prevalence of lactose intolerance (LI) using a lactose breath test when consuming a dose of 20 g of lactose in patients with gastrointestinal symptoms and in volunteers who do not show gastrointestinal problems. Part of the research was also to examine the symptoms that led the individual to see a doctor and undergo a lactose breath test. The method consisted in the evaluation of the results of breath tests, which were performed on patients and volunteers in the gastroenterological laboratory of the 1st Medical Faculty of Charles University and the General Hospital. A questionnaire was given to everyone to evaluate the symptoms of LI and the phenomena leading to malabsorption. Lactose intolerance was confirmed in 92 % of patients and 79 % of volunteers. The most reported symptoms in the patient sample were typical gastrointestinal LI-related problems: gastric and intestinal problems after milk consumption (85 % of patients), gastric and intestinal problems after consumption of dairy products (85 % of patients),...

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Krajíček, Tomáš. "Patelofemorální bolestivý syndrom a jeho ovlivnění patelárním tapingem." Master's thesis, 2008. http://www.nusl.cz/ntk/nusl-291353.

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Diploma thesis "Patellofemoral pain syndrome and its affection by patellar taping" consists of two parts. The first general part summarises all present knowledge in the field of classification, diagnostics and therapy of this syndrome. The experimental part investigates a partial area of conservative therapy and a patellar taping technique according to McConnell. With the use of EMG records of a group of patients with patellofemoral pain syndrome it assesses the immediate effect of patellar taping on the pain intesity and timing of m. vastus medialis obliquus to m. vastus lateralis throughout three different movements. Powered by TCPDF (www.tcpdf.org)

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Maturová, Barbora. "Diagnostické a terapeutické postupy v intervenci u osob s afázií." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-445865.

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The submitted diploma thesis deals with the topic of diagnostic and therapeutic procedures in speech therapy intervention for people with aphasia. The work is divided into four chapters, while the first three chapters are theoretically oriented the fourth chapter represents the empirical part of the work. In the first chapters, the diploma thesis deals with the theoretical definition of individual areas which are needed to understand the researched issues, specifically focuses on the concept of communication, explains the connection between brain and speech and, last but not least, defines the period of adulthood and old age. Concurrently this chapter presents the principles of speech therapy intervention with a focus on providing speech therapy intervention in the Czech Republic. The focus of the theoretical part of the thesis is the second chapter, which provides a direct (detailed) insight into the issue of aphasia as a neurogenic disorder of speech communication. The chapter deals with aphasia in terms of definition, etiology, symptomatology and classification approaches. Withal it represents a speech therapy intervention for people with a neurogenic speech disorder, aimed at diagnosis, therapy and prevention. The third theoretical chapter introduces the psychological and social aspects related...

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Špinková, Tereza. "Syndrom CSA u dětí." Master's thesis, 2011. http://www.nusl.cz/ntk/nusl-295975.

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Thesis CSA syndrome in children, examines current issues of sexual abuse and its consequences. The theoretical section deals with the history of sexual abuse, contemporary view on this issue and the legal treatment of offenders and victims. Furthermore, thesis acquaints readers with various types of sexual abuse and marginally with commercial abuse. The aim of the research in the practical section of the thesis is to ascertain the level of awarness of students in this issues, together with their own personal experience concerning sexual abuse and whether they have any sexually abused person in their vicinity. The quantitative research was conducted at the elementary school Drtinova.

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Lemb, Johanna Berta. "Therapie und Nachsorge des differenzierten Schilddrüsenkarzinoms: Eine Risikofaktoren-basierte Analyse der Göttinger Patienten seit 1990." Doctoral thesis, 2010. http://hdl.handle.net/11858/00-1735-0000-0006-AF87-1.

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Voleský, Kryštof. "Analýza rizikových faktorů pro vznik tendinopatií u běžců - literární rešerše." Master's thesis, 2021. http://www.nusl.cz/ntk/nusl-447443.

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Title: The analysis of risk factors for tendinopathy in runners Objectives: The aim of this thesis is to find out the most important risk factor for development of Achilles tendinopathy, detect the most relevant diagnostic approach with preventive monitoring for Achilles tendinopathy and evaluate the best treatment for Achilles tendinopathy in runners. Methods: The diploma thesis is in form of literary review. The Scopus, PubMed and Web of science databases were used to search for studie using a combination of keywords. A total of 1084 titles were identified. Due to duplicates 402 titles were excluded. On the basis of the name of the article 51 titles were included. On the basis of the availability 50 titles were included. On the basis of the abstract 46 titles were included. Finally 29 titles were included in this review. Results: The most important risk factor for development of Achilles tendinopathy was training intensity for distances 1500-3000 m, week running volume >65 km or being new to running. The most relevant diagnostic approach for Achilles tendinopaty was clinical examination assessing pain (Achilles tendon pain, positive palpation test) and function (pain during physical aktivity) of Achilles tendon. This approach serves as indication for sonographic assessment of Achilles tendon...

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Rosová, Anna. "Roztroušená skleróza: kortikoidní a biologická léčba, význam pedagogiky v rehabilitaci." Master's thesis, 2016. http://www.nusl.cz/ntk/nusl-343261.

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The submitted thesis deals with the theme of a neurodegenerative autoimmune disease, the sclerosis multiplex. Although this serious disease has become an object of intensive research in recent decades and there is an inexhaustible quantity of literature available, we haven't yet fully identified the causes of this disease as well as we haven't found such kind of effective treatment that would lead to the permanent recovery of patients. I focused my interest on two main spheres: first, the description and comparison of practices in terms of pharmacotherapy giving priority to its benefits to patients, second, the view of another important but not always appreciated part of therapy, physiotherapy or rehabilitation; the main point is studying of processes and actions from the pedagogical point of view.

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Bejšáková, Kristina. "Využití pomůcek systému pedagogiky Marie Montessori u osob s Downovým syndromem." Master's thesis, 2018. http://www.nusl.cz/ntk/nusl-372656.

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This thesis examines how can Montessori teaching aids be used for educating persons with Down syndrome. It describes Down syndrome and mental disability that is associated with it, listing also the options for educating persons with Down syndrome by development stage. It provides an overview of Montessori pedagogics, its history, concepts and principles, and its status in the Czech Republic. It classifies Montessori pedagogics in terms of general didactics theory, and introduces Montessori therapy. The author connects Montessori to other human- centric didactic and pedagogical systems, such as structured teaching, global reading approach, Reuven Feuerstein's Instrumental Enrichment, sensory activation, basal stimulation, and psychobiography. She explains the role of teaching aids in the Montessori system and describes the materials she is using in her lessons for persons with Down syndrome. The aim and structure of such lessons are described, together with how to work with clients and their families. The author emphasizes the importance of entry diagnostics, and presents three case studies of her clients. Teaching Aids Book is attached to the thesis, presenting 92 aids. For each teaching aid, it includes a description, modifications aimed for the use of clients with Down syndrome, and diagnostic...

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Šobáňová, Jindřiška. "Vliv fyziotepie na funkci posturální motoriky u výkonnostních sportovců (tenistů)." Master's thesis, 2008. http://www.nusl.cz/ntk/nusl-295205.

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This thesis, entitled "The effect of physiotherapy on the function of postural motorics in competitive sportsmen (tennis players)", deals with how the activation of the deep stabilizing muscles affects postural functions in adolescent tennis players. A group of 13 competitive tennis players, aged between 10 and 17 years, were used as our probands. These subjects were examined with a bespoke set of tests of functions and then they were individually educated to train with a set of stabilization functions. After the therapy, they were re-examined with this set of tests again and the results of both sets of tests were compared and evaluated. In particular, we evaluated the changes in reactions of individual players, the changes in separate tests and in groups of tests, and the position of players in match rankings. Powered by TCPDF (www.tcpdf.org)

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Dissertations / Theses on the topic 'Biocybernetic diagnostics and therapy'

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