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Journal articles on the topic 'Biodentine™'

Author: Grafiati
Published: 4 June 2021
Last updated: 25 April 2022

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1

Abuarqoub, Duaa, Nazneen Aslam, Hanan Jafar, Zakariya Abu Harfil, and Abdalla Awidi. "Biocompatibility of Biodentine™ ® with Periodontal Ligament Stem Cells: In Vitro Study." Dentistry Journal 8, no. 1 (February 8, 2020): 17. http://dx.doi.org/10.3390/dj8010017.

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Biodentine™ is a tricalcium silicate-based cement material that has a great impact on different biological processes of dental stem cells, compared to other biomaterials. Therefore, we aimed to investigate the optimum biocompatible concentration of Biodentine™ with stem cells derived from periodontal ligament (hPDLSCs) by determining cell proliferation, cytotoxicity, migration, adhesion and mineralization potential. hPDLSCs were treated with Biodentine™ extract at different concentrations; 20, 2, 0.2 and 0.02 mg/mL. Cells cultured without Biodentine™ were used as a blank control. The proliferation potential of hPDLSCs was evaluated by MTT viability analysis for 6 days. Cytotoxicity assay was performed after 3 days by using AnnexinV/7AAD. Migration potential was investigated by wound healing and transwell migration assays at both cellular and molecular levels. The expression levels of chemokines CXCR4, MCP-1 and adhesion molecules FGF-2, FN, VCAM and ICAM-1 were measured by qPCR. The communication potentials of these cells were determined by adhesion assay. In addition, mineralization potential was evaluated by measuring the expression levels of osteogenic markers; ALP, OCN, OPN and Collagen type1 by qPCR. Our results showed significant increase in the proliferation of hPDLSCs at low concentrations of Biodentine™ (2, 0.2 and 0.02 mg/mL) while higher concentration (20 mg/mL) exhibited cytotoxic effect on the cells. Moreover, 2 mg/mL Biodentine™ showed a significant increase in the migration, adhesion and mineralization potentials of the derived cells among all concentrations and when compared to the blank control. Our findings suggest that 2 mg/mL of Biodentine™ is the most biocompatible concentration with hPDLSCs, showing a high stimulatory effect on the biological processes.
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2

Aljdaimi, Abtesam, Hugh Devlin, and Mark Dickinson. "Effect of the Er: YAG laser on the shear bond strength of conventional glass ionomer and BiodentineTM to dentine." European Journal of Dentistry 12, no. 03 (July 2018): 380–85. http://dx.doi.org/10.4103/ejd.ejd_410_17.

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ABSTRACTObjectives: The purpose of this study was to determine if Er: YAG laser etching improves the shear bond strength (SBS) of Biodentin™ and GC Fuji IX® to dentine. Materials and Methods: Forty human dentine specimens were standardized and embedded in stone. The specimens were randomized into four groups (n = 10). Twenty samples were treated with the Er: YAG laser radiation and 10 of these restored with GC Fuji IX® and 10 with Biodentine™. The remaining 20 specimens acted as controls (no laser treatment); 10 were restored with GC Fuji IX® and 10 with Biodentin™. All samples were then stored in an incubator at 37.5°C and 100% humidity for 7 days. The SBS was determined using a Zwick universal testing machine. A two-way analysis of variance test was used to evaluate the statistical difference in SBS between the groups. An independent sample t-test was used to determine the statistical significance of differences between control and lased groups within the same material. Results: A highly statistically significant difference in SBS was found with the laser treatment (P = 0.0001) and material (i.e., Biodentin™ or Fuji IX® (P = 0.0001). The GC Fuji IX® group recorded the highest mean SBS required to dislodge the material from the laser-treated dentine surface (1.77 ± 0.22 Mega-Pascal [MPa]). The mean SBS of Biodentin™ to dentine following the laser radiation (1.12 ± 0.16 MPa) was significantly greater compared to the nonlased dentine (0.53 ± 0.09). Pearson Chi-square test indicated a nonsignificant relation between shear strength and mode of failure (P = 0.467). Conclusion: Laser etching of the dentine surfaces yielded a significant increase in the bond strength for both GC Fuji IX® and Biodentin™. The SBS of Biodentin™ to dentine is greater than with conventional glass ionomer (Fuji IX®).
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Paula, Anabela, Mafalda Laranjo, Carlos Miguel Marto, Ana Margarida Abrantes, João Casalta-Lopes, Ana Cristina Gonçalves, Ana Bela Sarmento-Ribeiro, Manuel M. Ferreira, Maria Filomena Botelho, and Eunice Carrilho. "Biodentine™ Boosts, WhiteProRoot®MTA Increases and Life® Suppresses Odontoblast Activity." Materials 12, no. 7 (April 11, 2019): 1184. http://dx.doi.org/10.3390/ma12071184.

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(1) Background: When pulp exposure occurs, reparative dentinogenesis can be induced by direct pulp capping to maintain the vitality and function of the tissue. The aim of this work was to assess the cytotoxicity and bioactivity of three different direct pulp capping materials, calcium hydroxide (Life®), mineral trioxide aggregate (WhiteProRoot®MTA) and calcium silicate (Biodentine™), in an odontoblast-like mouse cell line (MDPC-23). (2) Methods: Metabolic activity was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test (MTT)assay, viability by the sulforhodamine B (SRB) assay, and the type of death and cell cycle analysis by flow cytometry. Alkaline phosphatase was evaluated by polymerase chain reaction (PCR), and dentin sialoprotein expression was assessed by immunocytochemistry. Mineralization was determined by the Alizarin Red S colorimetric assay and quantified by spectrophotometry. (3) Results: Life® induced a decrease in metabolic activity and viability, which is associated with an increase cell death. WhiteProRoot®MTA and Biodentine™ induced similar effects in cytotoxicity assays, with an increase in the expression of dentin sialoprotein (DSP) and formation of mineralized deposits, especially with Biodentine™. (4) Conclusions: The results of WhiteProRoot®MTA confirm its indication for these therapies, justifying its recognition as the “gold standard”. Biodentine™ may be an alternative, since they promote the same cellular response that mineral trioxide aggregate (MTA) does.
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4

Poplai, Gaurav, and Sameer Jadhav. "Effect of Acidic Environment on the Push-out Bond Strength of Biodentine™." World Journal of Dentistry 3, no. 4 (2012): 313–15. http://dx.doi.org/10.5005/jp-journals-10015-1180.

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ABSTRACT Introduction An apical sealing material may be exposed to an inflamed environment with low pH levels. The change in physical and chemical properties of mineral trioxide aggregate has been well-documented in the presence of low pH. However, on literature search there is no documentation of the effect of low pH on Biodentine™. Aim To compare the effects of various levels of acidic pH on the push-out bond strength of Biodentine™. Materials and methods Forty root dentin slices from single rooted human teeth were sectioned horizontally. The canal lumens were instrumented to obtain a standardized diameter of 1.3 mm and filled with Biodentine™. The specimens were then randomly divided into four groups (n = 10) and wrapped in pieces of gauze soaked in phosphate buffer saline solution (pH = 7.4) and butyric acid buffered at pH values of 4.4, 5.4 and 6.4 respectively. They were incubated at 37°C for 4 days. Push-out test were then carried out using universal testing machine. The data was analyzed using one-way analysis of variance (ANOVA) technique with Tukey's correction for multiple group comparisons. Results Data was subjected to one-way ANOVA using Tukey's post hoc test. Group I (control, pH = 7.4) showed greatest bond strength of 19.5 ± 0.9 MPa. The least push-out bond strength of 11.7 ± 0.5 MPa was observed in Group IV where the specimens were soaked at pH 4.4. A p-value less than 0.05 were considered to be statistically significant. Conclusion The force needed to displace Biodentine was significantly lower in samples stored at lower pH values. How to cite this article Poplai G, Jadhav S, Hegde V. Effect of Acidic Environment on the Push-out Bond Strength of Biodentine™. World J Dent 2012;3(4):313-315.
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5

Suárez-Franco, J. L., B. I. Cerda-Cristerna, A. Suárez-Porras, R. E. Flores-Ventura, M. Trujillo-Hernández, and R. Trueba-García. "Physicochemical study of Biodentine™-dentine tissue interphase." Dental Materials 35 (2019): e39. http://dx.doi.org/10.1016/j.dental.2019.08.079.

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Paula, Anabela, Eunice Carrilho, Mafalda Laranjo, Ana M. Abrantes, João Casalta-Lopes, Maria Filomena Botelho, Carlos Miguel Marto, and Manuel M. Ferreira. "Direct Pulp Capping: Which is the Most Effective Biomaterial? A Retrospective Clinical Study." Materials 12, no. 20 (October 16, 2019): 3382. http://dx.doi.org/10.3390/ma12203382.

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(1) Background: Recently, tricalcium silicate cements, such as Biodentine™, have emerged. This biomaterial has a calcium hydroxide base and characteristics like mineral aggregate trioxide cements, but has tightening times that are substantially more suitable for their application and other clinical advantages. (2) Methods: A retrospective clinical study was conducted with 20 patients, which included a clinical evaluation of the presence or absence of pulp inflammation compatible symptoms, radiographic evaluation of the periapical tissues, and structural alterations of the coronary restoration that supports pulp capping therapies with Biodentine™ and WhiteProRoot®MTA. (3) Results: This clinical study revealed similar success rates between mineral trioxide cement and tricalcium silicates cements at 6 months, with 100% and 95% success rates, respectively. There were no statistically significant differences between both biomaterials and between these and the various clinical circumstances, namely the absolute isolation of the operating field, exposure size, the aetiology of exposure, and even the type of restorative material used. (4) Conclusions: Biodentine™ demonstrated a therapeutic effect on the formation of a dentin bridge accompanied by slight inflammatory signs, with a high clinical success rate, indicating the possibility of its effective and safe use in dental pulp direct capping in humans, similar to the gold standard material.
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Chinadet, W., T. Sutharaphan, and P. Chompu-inwai. "Biodentine™ Partial Pulpotomy of a Young Permanent Molar with Signs and Symptoms Indicative of Irreversible Pulpitis and Periapical Lesion: A Case Report of a Five-Year Follow-Up." Case Reports in Dentistry 2019 (September 12, 2019): 1–5. http://dx.doi.org/10.1155/2019/8153250.

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The purpose of this paper was to report the five-year success of Biodentine™ partial pulpotomy in a young permanent molar, with signs and symptoms indicative of irreversible pulpitis and periapical lesion, in a nine-year-old girl. Preoperative clinical examination revealed a large carious lesion of the left mandibular permanent first molar. The patient reported pain on percussion. The tooth responded positively to the electric pulp test and had lingering pain after cold testing. A periapical radiograph showed a deep carious lesion and periapical lesion. Based on the clinical and radiographical examination, the tooth had signs and symptoms indicative of irreversible pulpitis and periapical lesion. During caries removal, pulp exposure occurred, and 2-3 mm in depth of pulp tissue at the exposure site was removed. Haemorrhage was controlled within four minutes with 2.5% sodium hypochlorite-moistened cotton pellets. Biodentine™ was then applied as both a pulp dressing and a temporary restoration. At the following visit, composite resin was placed over the Biodentine™ as a final restoration. During a five-year follow-up, the tooth was asymptomatic, had positive responses to sensibility tests, and had no discolouration. Follow-up radiographs showed a dentine bridge and periapical healing.
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Brenes-Valverde DDS, Karen, Elian Conejo-Rodríguez PhD, José Roberto Vega-Baudrit PhD, Mauricio Montero-Aguilar MSc, and Daniel Chavarría-Bolaños MSc, PhD. "Evaluation of Microleakage by Gas Permeability and Marginal Adaptation of MTA and Biodentine™ Apical Plugs: In Vitro Study." Odovtos - International Journal of Dental Sciences 20, no. 1 (November 16, 2017): 57–67. http://dx.doi.org/10.15517/ijds.v0i0.28952.

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The endodontic treatment of teeth with incomplete development is always a complex task. Nowadays, biomaterials such as bioceramics offers promising clinical evidence that supports its use. However, the standardization of its use for apexification purpose still needs a deeper understanding of the materials’ behavior. The aim of this investigation was to evaluate the marginal adaptability and microleakage by gas permeability of MTA and Biodentine™ apical plugs in an in vitro model. Materials and methods: Twenty-four single rooted human teeth were selected according to previously stablished inclusion criteria. All samples were prepared obtaining standard cylindrical internal canals with a diameter of 1.3 mm. Root canals were gently rinsed using 5.25% sodium hypochlorite and EDTA 17%. The apical 3mm and remaining coronal dental structure were sectioned to obtain 10mm roots. Roots were randomly assigned to 3 different groups as follows: GROUP A: MTA (n=10), GROUP B: Biodentine™ (n=10) and Group C: Control (positive n=1, negative n=3). MTA and Biodentine™ were prepared according to manufacturer’s indications, and apical plugs of 4mm were passively placed in the correspondent teeth. All samples were stored in saline solution for 7 days at 37°C before evaluation. Samples were mounted in cylindrical sample-holders using epoxy resin. Microleakage was evaluated with an automatic permeability detector that calculates nitrogen diffusion between the material-root interphase. After microleakage evaluation, the samples were recovered and analyzed by scanning electron microscopy (SEM). Microleakage results were analyzed using Chi-square and adaptation was evaluated with a descriptive analysis. Results: None of the evaluated materials completely avoided the nitrogen microleakage (positive leakage of 10% and 20% of samples for MTA and Biodentine™ respectively); with no statistical significant difference between groups (p=0.527). All apical plugs showed good adaptation under SEM, at 30x, 200x, 1000x and 2500x; with microscopical structures similar to previous reports. Conclusions: Both bioceramics behave similar when used as apical barriers to avoid permeability, with acceptable marginal adaptation. Further in vivo studies are needed to validate these results.
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Brenes-Valverde DDS, Karen, Elian Conejo-Rodríguez PhD, José Roberto Vega-Baudrit PhD, Mauricio Montero-Aguilar MSc, and Daniel Chavarría-Bolaños MSc, PhD. "Evaluation of Microleakage by Gas Permeability and Marginal Adaptation of MTA and Biodentine™ Apical Plugs: In Vitro Study." Odovtos - International Journal of Dental Sciences 20, no. 1 (November 16, 2017): 57–67. http://dx.doi.org/10.15517/ijds.v20i1.28952.

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The endodontic treatment of teeth with incomplete development is always a complex task. Nowadays, biomaterials such as bioceramics offers promising clinical evidence that supports its use. However, the standardization of its use for apexification purpose still needs a deeper understanding of the materials’ behavior. The aim of this investigation was to evaluate the marginal adaptability and microleakage by gas permeability of MTA and Biodentine™ apical plugs in an in vitro model. Materials and methods: Twenty-four single rooted human teeth were selected according to previously stablished inclusion criteria. All samples were prepared obtaining standard cylindrical internal canals with a diameter of 1.3 mm. Root canals were gently rinsed using 5.25% sodium hypochlorite and EDTA 17%. The apical 3mm and remaining coronal dental structure were sectioned to obtain 10mm roots. Roots were randomly assigned to 3 different groups as follows: GROUP A: MTA (n=10), GROUP B: Biodentine™ (n=10) and Group C: Control (positive n=1, negative n=3). MTA and Biodentine™ were prepared according to manufacturer’s indications, and apical plugs of 4mm were passively placed in the correspondent teeth. All samples were stored in saline solution for 7 days at 37°C before evaluation. Samples were mounted in cylindrical sample-holders using epoxy resin. Microleakage was evaluated with an automatic permeability detector that calculates nitrogen diffusion between the material-root interphase. After microleakage evaluation, the samples were recovered and analyzed by scanning electron microscopy (SEM). Microleakage results were analyzed using Chi-square and adaptation was evaluated with a descriptive analysis. Results: None of the evaluated materials completely avoided the nitrogen microleakage (positive leakage of 10% and 20% of samples for MTA and Biodentine™ respectively); with no statistical significant difference between groups (p=0.527). All apical plugs showed good adaptation under SEM, at 30x, 200x, 1000x and 2500x; with microscopical structures similar to previous reports. Conclusions: Both bioceramics behave similar when used as apical barriers to avoid permeability, with acceptable marginal adaptation. Further in vivo studies are needed to validate these results.
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Alotaibi, J., S. Saji, and M. V. Swain. "FTIR characterization of the setting reaction of biodentine™." Dental Materials 34, no. 11 (November 2018): 1645–51. http://dx.doi.org/10.1016/j.dental.2018.08.294.

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11

Mutluay, Merve, and Abidin Mutluay. "Sealing efficiency of MTA, accelerated MTA, Biodentine and RMGIC as retrograde filling materials." Balkan Journal of Dental Medicine 25, no. 3 (2021): 159–65. http://dx.doi.org/10.2478/bjdm-2021-0025.

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Background/Aim: This in-vitro study evaluated the apical-sealing ability of MTA, MTA+10% CaCl2, Biodentine™ and RMGIC when used as retrograde material. Material and Methods: A total of 80 single-rooted bovine incisors were decoronated, instrumented, and divided into 4 groups according to retrograde material, as follows: Group 1: MTA (ProRoot MTA, Dentsply); Group 2: MTA (ProRoot MTA, Dentsply) + 10% CaCl2; Group 3: Biodentine (Biodentine®, Septodont); Group 4: RMGIC (Nova Glass - LC, Imicryl). Root surfaces were isolated with nail Polish, and teeth were immersed in 2% methylene blue dye at 37°C for 48 h. The extent of dye penetration (mm) was measured under a stereomicroscope. Results: RMGIC had the highest mean-rank dye penetration score (MP=49.05), followed by MTA (MP=43.65), Biodentine (35.95) and MTA+CaCl2 (MP=33.35). The results of paired comparison tests found the mean microleakage value of MTA+10% CaCl2 and Biodentine (Group 3) to be significantly lower than that of RMGIC (respectively; p=0,020, p=0,019). No significant difference was found in the other group comparisons (p> 0.05). Conclusions: These results suggest that the addition of an accelerator to MTA may reduce microleakage following endodontic surgery. Biodentine can be used as an alternative to MTA on retrograde obturation.
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Trujillo-Hernández, Margarita, Rebeca E. Flores-Ventura, Angélica Suárez-Porras MSc, Leandro García-González PhD, Julián Hernández-Torres PhD, Luis Zamora-Peredo PhD, and José Luis Suárez-Franco DDS, MSc, PhD. "Comparative Study of the Bioactivity of Two Bioceramic Materials." Odovtos - International Journal of Dental Sciences 21, no. 2 (August 31, 2018): 73–81. http://dx.doi.org/10.15517/ijds.v21i2.37061.

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Calcium silicate-based materials have been shown to be bioactive due to their ability to produce biologically compatible carbonated apatite. The objective of this study was to analyze the bioactivity of Biodentine ™ and MTA Repair HP® in contact with human dentine discs, which were sealed and divided randomly to form four groups: group 1 Biodentine™, group 2 MTA Repair HP®, positive control group MTA Angelus® and negative control group IRM®, which were incubated in PBS solution for 10 days, for a subsequent analysis by means of MEB-EDS and Raman spectroscopy. The three calcium-based materials analyzed in this study proved to be bioactive because upon contact with a phosphate-based solution they were triggered at the onset of amorphous calcium phosphate, as the precursor during the formation of carbonated apatite.
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Alhowaish, Latifa, Fouad Salama, Mohammed Al-Harbi, and Mohamad Abumoatti. "Shear Bond Strength of a Resin Composite to Six Pulp Capping Materials Used in Primary Teeth." Journal of Clinical Pediatric Dentistry 44, no. 4 (August 1, 2020): 234–39. http://dx.doi.org/10.17796/1053-4625-44.4.4.

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Aim: The purpose of this in vitro study was to assess the shear bond strength (SBS) and bond failure types of a resin-composite to six pulp-capping materials used in primary teeth. Study design: Eight-disc specimens from each pulp-capping material (6 groups) to bond to Filtek™ Z350 XT Flowable using a standard PVC tube (2×2mm). All groups were prepared according to the instruction of the manufacturer. The SBS was measured with a crosshead speed of 0.5 mm/min using a universal testing machine. Failure mode evaluation was completed using Digital Microscope by two independent examiners. Results: Urbical LC® showed the highest SBS (Mean±SD) followed by ProRoot® MTA and TheraCal LC® (35.422±2.910, 22.114±2.515, and 21.175±1.983) respectively. ANOVA showed significant differences between all groups (P=0.0001). Urbical LC® and Photac™ Fil QuickAplicap™ were statistically significant different from all other pulp-capping materials groups. ProRoot® MTA was statistically significant different than Biodentine® (P=0.0001) and Photac™ Fil (P=0.0001). The total number of bond failure was recorded for cohesive B failure/cohesive in the pulp-capping material (14) and adhesive failure (14). Conclusion: Most of the tested pulp-capping materials bonded to Filtek™ Z350 XT demonstrated clinically acceptable and high SBS. Urbical LC showed the highest SBS while Biodentine® showed the lowest SBS.
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Berkathullah, Marwah, Mohideen S. Farook, and Okba Mahmoud. "The Effectiveness of Remineralizing Agents on Dentinal Permeability." BioMed Research International 2018 (September 12, 2018): 1–12. http://dx.doi.org/10.1155/2018/4072815.

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The effectiveness of remineralizing agents in reducing dentine permeability by tubule occlusion using fluid filtration device functioning at 100 cmH2O (1.4 psi) pressure and SEM/EDX analysis were evaluated and compared. Seventy (n = 70) dentine discs of 1±0.2 mm width were prepared from sound permanent human molars. Fifty (n = 50) dentine discs were randomly divided into 5 groups (n = 10): Group 1: GC Tooth Mousse Plus (Recaldent GC Corporation Tokyo, Japan), Group 2: Clinpro™ White Varnish (3M ESPE, USA), Group 3: Duraphat® Varnish (Pharbil Waltrop GmbH, Germany), Group 4: Colgate Sensitive Pro-Relief™ dentifrice (Colgate Palmolive, Thailand), and Group 5: Biodentine™ (Septodont/UK). Dentine permeability was measured after treatment application at 10 minutes, artificial saliva immersion at 7 days, and citric acid challenge for 3 minutes. Data were analyzed by two-way repeated measures ANOVA. Dentine specimens (n = 20) were used for SEM/EDX analyses to obtain qualitative results on dentine morphology and surface deposits. Each treatment agent significantly reduced dentine permeability immediately after treatment application and created precipitates on treated dentine surfaces. All agents increased permeability values after 7 days of artificial saliva immersion except Clinpro White Varnish and Biodentine. Clinpro White Varnish exhibited significant resistance to acid challenge compared to others. Colgate Sensitive Pro-Relief dentifrice has a dual mechanism of action in reducing the dentine sensitivity.
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Talabani, Ranjdar Mahmood, Balkees Taha Garib, and Reza Masaeli. "Bioactivity and Physicochemical Properties of Three Calcium Silicate-Based Cements: An In Vitro Study." BioMed Research International 2020 (May 27, 2020): 1–10. http://dx.doi.org/10.1155/2020/9576930.

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Objective. This study evaluated the bioactivity and physicochemical properties of three commercial calcium silicate-based endodontic materials (MTA, EndoSequence Root Repair Material putty, and Biodentine™). Material and Methods. Horizontal sections of 3 mm thickness from 18 root canals of human teeth were subjected to biomechanical preparation with WaveOne Gold large rotary instruments. The twelve specimens were filled with three tested materials (MM-MTA, EndoSequence Root Repair Material putty, and Biodentine™) and immersed in phosphate-buffered saline for 7 and 30 days. After this period of time, each specimen of each material was processed for morphological observation, surface precipitates, and interfacial dentin using SEM. In addition, the surface morphology of the set materials, without soaking in phosphate-buffered solution after one day and after 28 days stored in phosphate-buffered saline, was evaluated using SEM; also, the pH of the soaking water and the amount of calcium ions released from the test materials were measured by using an inductively coupled plasma-optical emission spectroscopy test. Data obtained were analyzed using one-way analysis of variance and Tukey’s honest significant difference test with a significance level of 5%. Result. The formation of precipitates was observed on the surfaces of all materials at 1 week and increased substantially over time. Interfacial layers in some areas of the dentin-cement interface were found from one week of immersion. All the analyzed materials showed alkaline pH and capacity to release calcium ions; however, the concentrations of released calcium ions were significantly more in Biodentine and ESRRM putty than MM-MTA (P<0.05). ESRRM putty maintained a pH of around 11 after 28 days. Conclusion. Compared with MM-MTA, Biodentine and ESRRM putty showed significantly more calcium ion release. However, exposure of three tested cements to phosphate-buffered solution resulted in precipitation of apatite crystalline structures over both cement and dentin that increased over time. This suggests that the tested materials are bioactive.
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Dhanyakumar, NM, and Soumya R. Devasia. "Management of Palatogingival Groove using the Innovative Tricalcium Silicate Material Biodentine™." CODS Journal of Dentistry 8, no. 1 (2016): 54–58. http://dx.doi.org/10.5005/jp-journals-10063-0013.

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ABSTRACT Palatogingival grooves (PGG) are developmental malformations that cause endodontic–periodontal lesions. Owing to their inconspicuous occurrence, funnel-shaped morphology, and variable extent on tooth root, they promote adherence of plaque and bacteria to levels significant for the development of pathology. Several treatment approaches have been recognized in the literature for the management of this anomaly. Here, in this report, a 32-year-old female patient reported with the complaint of pain and swelling in maxillary left lateral incisor. Clinical examination confirmed an endodontic–periodontal lesion in relation to PGG. Endodontic treatment was instituted followed by odontoplasty of the groove and restoration with newer calcium silicate cement, Biodentine. Combined endodontic–periodontal approach was successful in resolving the pathology with complete healing seen both clinically and radiographically. Timely diagnosis, prevention, and management are highly recommended to prevent tooth loss due to complications arising secondary to their presence. How to cite this article Dhanyakumar NM, Devasia SR. Management of Palatogingival Groove using the Innovative Tricalcium Silicate Material Biodentine™. CODS J Dent 2016;8(1):54-58.
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Jung, Yoonsun, Ji-Young Yoon, Kapil Dev Patel, Lan Ma, Hae-Hyoung Lee, Jongbin Kim, Jung-Hwan Lee, and Jisun Shin. "Biological Effects of Tricalcium Silicate Nanoparticle-Containing Cement on Stem Cells from Human Exfoliated Deciduous Teeth." Nanomaterials 10, no. 7 (July 14, 2020): 1373. http://dx.doi.org/10.3390/nano10071373.

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Nanomaterials can enhance interactions with stem cells for tissue regeneration. This study aimed to investigate the biological effects of tricalcium silicate nanoparticle-containing cement (Biodentine™) during or after setting on stem cells from human exfoliated deciduous teeth (SHED) to mimic clinically relevant situations in which materials are adapted. Specimens were divided into four groups depending on the start of extraction time (during (3, 6 and 12 min) or after setting (24 h)) and extracted in culture medium for 24 h for further physicochemical and biological analysis. After cell viability in serially diluted extracts was evaluated, odontogenic differentiation on SHED was evaluated by ARS staining using nontoxic conditions. A physicochemical analysis of extracts or specimens indicated different Ca ion content, pH, and surface chemistry among groups, supporting the possibility of different biological functionalities depending on the extraction starting conditions. Compared to the ‘after setting’ group, all ‘during setting’ groups showed cytotoxicity on SHED. The during setting groups induced more odontogenic differentiation at the nontoxic concentrations compared to the control. Thus, under clinically simulated extract conditions at nontoxic concentrations, Biodentine™ seemed to be a promising odontoblast differentiating biomaterial that is helpful for dental tissue regeneration. In addition, to simulate clinical situations when nanoparticle-containing cement is adjusted, biological effects during setting need to be considered.
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Rajasekharan, S., L. C. Martens, R. G. E. C. Cauwels, and R. M. H. Verbeeck. "Biodentine™ material characteristics and clinical applications: a review of the literature." European Archives of Paediatric Dentistry 15, no. 3 (March 11, 2014): 147–58. http://dx.doi.org/10.1007/s40368-014-0114-3.

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Savitri, D., E. Suprastiwi, and A. Margono. "Applying glass ionomer cement to MTA flow™ and biodentine™ and its effects on the interface layer." Journal of Physics: Conference Series 884 (August 2017): 012109. http://dx.doi.org/10.1088/1742-6596/884/1/012109.

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Pasqualini, Damiano, Allegra Comba, Laura Annaratone, Virginia Mola, Mario Alovisi, Lorenzo Breschi, Annalisa Mazzoni, Nicola Scotti, Paola Cassoni, and Elio Berutti. "Osteogenic Potential of Fast Set Bioceramic Cements: Molecular and In Vitro Study." Applied Sciences 10, no. 19 (September 25, 2020): 6713. http://dx.doi.org/10.3390/app10196713.

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Recently, pre-mixed bioceramics in fast set formulations have been increasingly utilized in clinical practice as an alternative to mineral trioxide aggregate (MTA) for their shorter setting time and better handling properties. However, the impact on their osteogenic potential, due to modifications in chemical composition to promote a fast setting, is still unclear. This molecular and in vitro study compared the osteogenic potential of root repairing material putty fast set (FSP) with root-repairing material putty (RRMPU), root-repairing material paste (RRMPA), Biodentine™ and MTA. The null hypothesis tested was that there are no differences among the tricalcium silicate materials in terms of osteogenic potential. Standardized discs were cultured with MG-63 human osteoblastic-like cells to assess biocompatibility, the activity of alkaline phosphatase (ALP) and osteogenic potential. Biocompatibility was evaluated at baseline and after 24 and 48 h. Osteogenic differentiation was assessed after 15 days. Data were analyzed with one-way ANOVAs and Tukey’s post-hoc test (p < 0.05). All materials showed biocompatibility and bioactivity. ALP activity, which induces mineral nodule deposition, increased in all the cements tested, with a significant increase in RRMPU (p < 0.001) and FSP (p < 0.001) samples versus MTA. In vitro mineralization was significantly increased for RRMPU (p < 0.0001), FSP (p = 0.00012) and Biodentine™ (p < 0.0001) versus MTA. The bioceramics tested showed higher levels of biocompatibility and bioactivity than MTA; a higher capacity for mineralization was observed with RRMPU and FSP versus MTA.
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Tran, Xuan, Hamideh Salehi, Minh Truong, Minic Sandra, Jeremy Sadoine, Bruno Jacquot, Frédéric Cuisinier, Catherine Chaussain, and Tchilalo Boukpessi. "Reparative Mineralized Tissue Characterization after Direct Pulp Capping with Calcium-Silicate-Based Cements." Materials 12, no. 13 (June 29, 2019): 2102. http://dx.doi.org/10.3390/ma12132102.

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Nowadays, the preservation of dental pulp vitality is an integral part of our daily therapies. The success of these treatments depends on the clinical situation as well as the biomaterials used. Mineral Trioxide aggregate and BiodentineTM are commonly used as pulp capping materials. One objective of vital pulp therapy is the repair/regeneration of the pulp. In addition to the initial inflammatory status of the pulp, the nature and quality of the new mineralized tissue obtained after pulp capping directly influence the success of the treatment. In order to characterize the reparative dentin, in the current study, the chemical composition and microstructure of the dentin bridge after direct pulp capping using Biodentine™ and mineral trioxide aggregate (MTA) was studied by using Raman microspectroscopy and scanning electron microscopy, respectively. The results showed that the reparative dentin bridge observed in both groups presented dentin tubules and chemical composition similar to primary dentin. With the limitations of this study, the calcium-silicate-based cements used as pulp capping materials provide an optimal environment for pulp healing, resulting in a reparative dentin resembling on certain points of the primary dentin and the regeneration of the pulp.
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Ayoub, Fouad, Hitaf Nasrallah, Balsam El Noueiri, and Charles Pilipili. "Clinical and Radiographic Evaluations of Biodentine™ Pulpotomies in Mature Primary Molars (Stage 2)." International Journal of Clinical Pediatric Dentistry 11, no. 6 (2018): 496–504. http://dx.doi.org/10.5005/jp-journals-10005-1564.

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Batista, Melissa, Jaime Portugal, Mário Polido, Mário Rito Pereira, and Ana Cristina Azul. "# 44. Resistência adesiva ao ProRoot® MTA e BIODENTINE™ com diferentes estratégias adesivas." Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial 56 (December 2015): 20. http://dx.doi.org/10.1016/j.rpemd.2015.10.045.

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Rajasekharan, S., L. C. Martens, R. G. E. C. Cauwels, and R. P. Anthonappa. "Biodentine™ material characteristics and clinical applications: a 3 year literature review and update." European Archives of Paediatric Dentistry 19, no. 1 (January 25, 2018): 1–22. http://dx.doi.org/10.1007/s40368-018-0328-x.

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Suprastiwi, Endang, Aditya Wisnu Putranto, and Ike Dwi Maharti. "The Ability of Biodentine™ of Guided Tissue Remineralization (GTR): Analysis Using SEM, EDX and TEM." Pesquisa Brasileira em Odontopediatria e Clínica Integrada 19, no. 1 (2019): 1–8. http://dx.doi.org/10.4034/pboci.2019.191.25.

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Luo, Zhirong, Dongmei Li, Meetu R. Kohli, Qing Yu, Syngcuk Kim, and Wen-xi He. "Effect of Biodentine™ on the proliferation, migration and adhesion of human dental pulp stem cells." Journal of Dentistry 42, no. 4 (April 2014): 490–97. http://dx.doi.org/10.1016/j.jdent.2013.12.011.

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Rajasekharan, S., L. C. Martens, R. G. E. C. Cauwels, R. P. Anthonappa, and R. M. H. Verbeeck. "Correction to: Biodentine™ material characteristics and clinical applications: a 3 year literature review and update." European Archives of Paediatric Dentistry 19, no. 2 (March 15, 2018): 129. http://dx.doi.org/10.1007/s40368-018-0335-y.

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Katge, FarhinA, PoojaRavindra Shivasharan, and Devendra Patil. "Sealing ability of mineral trioxide aggregate Plus™ and Biodentine™ for repair of furcal perforation in primary molars: An in vitro study." Contemporary Clinical Dentistry 7, no. 4 (2016): 487. http://dx.doi.org/10.4103/0976-237x.194100.

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de Sousa Reis, Magda, Roberta Kochenborger Scarparo, Liviu Steier, and José Antônio Poli de Figueiredo. "Periradicular inflammatory response, bone resorption, and cementum repair after sealing of furcation perforation with mineral trioxide aggregate (MTA Angelus™) or Biodentine™." Clinical Oral Investigations 23, no. 11 (March 12, 2019): 4019–27. http://dx.doi.org/10.1007/s00784-019-02833-z.

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Attik, G. N., C. Villat, F. Hallay, N. Pradelle-Plasse, H. Bonnet, K. Moreau, P. Colon, and B. Grosgogeat. "In vitrobiocompatibility of a dentine substitute cement on human MG63 osteoblasts cells: Biodentine™ versus MTA®." International Endodontic Journal 47, no. 12 (March 20, 2014): 1133–41. http://dx.doi.org/10.1111/iej.12261.

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Escobar-García, Diana María, Eva Aguirre-López, Verónica Méndez-González, and Amaury Pozos-Guillén. "Cytotoxicity and Initial Biocompatibility of Endodontic Biomaterials (MTA and Biodentine™) Used as Root-End Filling Materials." BioMed Research International 2016 (2016): 1–7. http://dx.doi.org/10.1155/2016/7926961.

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Objective. The aim of this study was to evaluate the cytotoxicity and cellular adhesion of Mineral Trioxide Aggregate (MTA) and Biodentine (BD) on periodontal ligament fibroblasts (PDL).Methods. PDL cells were obtained from nonerupted third molars and cultured; MTS cellular profusion test was carried out in two groups: MTA and BD, with respective controls at different time periods. Also, the LIVE/DEAD assay was performed at 24 h. For evaluation of cellular adhesion, immunocytochemistry was conducted to discern the expression of Integrinβ1 and Vinculin at 12 h and 24 h. Statistical analysis was performed by the Kruskal-Wallis and Mann-WhitneyUtests.Results. MTA and BD exhibited living cells up to 7 days. More expressions of Integrinβ1 and Vinculin were demonstrated in the control group, followed by BD and MTA, which also showed cellular loss and morphological changes. There was a significant difference in the experimental groups cultured for 5 and 7 days compared with the control, but there was no significant statistical difference between both cements.Conclusions. Neither material was cytotoxic during the time evaluated. There was an increase of cell adhesion through the expression of focal contacts observed in the case of BD, followed by MTA, but not significantly.
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Li, Qiu, Andrew P. Hurt, and Nichola J. Coleman. "The Application of 29Si NMR Spectroscopy to the Analysis of Calcium Silicate-Based Cement using Biodentine™ as an Example." Journal of Functional Biomaterials 10, no. 2 (May 30, 2019): 25. http://dx.doi.org/10.3390/jfb10020025.

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Biodentine is one of the most successful and widely studied among the second generation of calcium silicate-based endodontic cements. Despite its popularity, the setting reactions of this cement system are not currently well understood. In particular, very little is known about the formation and structure of the major calcium silicate hydrate (C-S-H) gel phase, as it is difficult to obtain information on this poorly crystalline material by the traditional techniques of powder X-ray diffraction analysis (XRD) and Fourier transform infrared spectroscopy (FTIR). In this study, the hydration reactions of Biodentine are monitored by XRD, FTIR, isothermal conduction calorimetry and, for the first time, 29Si magic angle spinning nuclear magnetic resonance spectroscopy (29Si MAS NMR) is used to investigate the structures of the anhydrous calcium silicate phases and the early C-S-H gel product. XRD analysis indicated that the anhydrous powder comprises 73.8 wt% triclinic tricalcium silicate, 4.45 wt% monoclinic β-dicalcium silicate, 16.6 wt% calcite and 5.15 wt% zirconium oxide. Calorimetry confirmed that the induction period for hydration is short, and that the setting reactions are rapid with a maximum heat evolution of 28.4 mW g−1 at 42 min. A progressive shift in the FTIR peak maximum from 905 to 995 cm−1 for the O-Si-O stretching vibrations accompanies the formation of the C-S-H gel during 1 week. The extent of hydration was determined by 29Si MAS NMR to be 87.0%, 88.8% and 93.7% at 6 h, 1 day and 1 week, respectively, which is significantly higher than that of MTA. The mean silicate chain length (MCL) of the C-S-H gel was also estimated by this technique to be 3.7 at 6 h and 1 day, and to have increased to 4.1 after 1 week. The rapid hydration kinetics of Biodentine, arising from the predominance of the tricalcium silicate phase, small particle size, and ‘filler effect’ of calcite and zirconium oxide, is a favorable characteristic of an endodontic cement, and the high values of MCL are thought to promote the durability of the cement matrix.
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Athanasiadou, Eirini, Maria Paschalidou, Anna Theocharidou, Nikolaos Kontoudakis, Konstantinos Arapostathis, and Athina Bakopoulou. "Biological interactions of a calcium silicate based cement (Biodentine™) with Stem Cells from Human Exfoliated Deciduous teeth." Dental Materials 34, no. 12 (December 2018): 1797–813. http://dx.doi.org/10.1016/j.dental.2018.09.014.

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Gad El-Rab, Sanaa M. F., Amal A. Ashour, Sakeenabi Basha, Amal Ahmed Alyamani, Nayef H. Felemban, and Enas Tawfik Enan. "Well-Orientation Strategy Biosynthesis of Cefuroxime-Silver Nanoantibiotic for Reinforced Biodentine™ and Its Dental Application against Streptococcus mutans." Molecules 26, no. 22 (November 12, 2021): 6832. http://dx.doi.org/10.3390/molecules26226832.

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Dental caries results from the bacterial pathogen Streptococcus mutans (S. mutans) and is the maximum critical reason for caries formation. Consequently, the present study aims to evaluate the antibacterial activity of a newly synthesized nanoantibiotic–Biodentine formulation. The silver nanoparticles (ROE-AgNPs) were biosynthesized from the usage of Rosmarinus officinalis L. extract (ROE) and conjugated with cefuroxime to form Cefuroxime-ROE-AgNPs. Using Biodentine™ (BIOD), five groups of dental materials were prepared, in which Group A included conventional BIOD, Group B included BIOD with ROE-AgNPs, Groups C and D included BIOD with Cefuroxime-ROE-AgNPs at concentrations of 0.5% and 1.5% cefuroxime, respectively, and Group E included BIOD with 1.5% cefuroxime. The synthesized ROE-AgNPs or Cefuroxime-ROE-AgNPs were characterized for conjugating efficiency, morphology, particle size, and in vitro release. Minimum inhibitory concentration (MIC) of the cefuroxime, ROE-AgNPs, and Cefuroxime-ROE-AgNPs were additionally evaluated against cefuroxime resistant S. mutans, which furthered antibacterial efficacy of the five groups of dental materials. The UV-Visible spectrum showed the ROE-AgNPs or Cefuroxime-ROE-AgNPs peaks and their formation displayed through transmission electron microscopy (TEM), X-ray diffraction (XRD) pattern, and Fourier transforms infrared (FTIR) analysis. The end result of Cefuroxime-ROE-AgNPs showed conjugating efficiency up to 79%. Cefuroxime-ROE-AgNPs displayed the highest antibacterial efficacy against S. mutans as compared to cefuroxime or ROE-AgNPs alone. Moreover, the MIC of ROE-AgNPs and Cefuroxime-ROE-AgNPs was detected against S. mutans to be 25 and 8.5 μg/mL, respectively. Consequently, Cefuroxime-ROE-AgNPs displayed that a decrease in the MIC reached to more than three-fold less than MIC of ROE-AgNPs on the tested strain. Moreover, Cefuroxime-ROE-AgNPs/BIOD was employed as a novel dental material that showed maximum antimicrobial activity. Groups C and D of novel materials showed inhibitory zones of 19 and 26 mm, respectively, against S. mutans and showed high antimicrobial rates of 85.78% and 91.17%, respectively. These data reinforce the utility of conjugating cefuroxime with ROE-AgNPs to retrieve its efficiency against resistant S. mutant. Moreover, the nanoantibiotic delivered an advantageous antibacterial effect to BIOD, and this may open the door for future conjugation therapy of dental materials against bacteria that cause dental caries.
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Suresh, Nandini, Buvaneshwari Arul, Dinesh Kowsky, and Velmurugan Natanasabapathy. "Successful Regenerative Endodontic Procedure of a Nonvital Immature Permanent Central Incisor Using Amniotic Membrane as a Novel Scaffold." Dentistry Journal 6, no. 3 (August 2, 2018): 36. http://dx.doi.org/10.3390/dj6030036.

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Successful regenerative endodontic procedure was performed in nonvital immature permanent central incisor (Stage-4 root development) using human amniotic membrane (HAM) as a novel scaffold. The treatment was performed according to the American Association of Endodontics guidelines with minimal canal instrumentation, 1% Sodium hypochlorite as irrigant and calcium hydroxide as intracanal medicament. During the second appointment, HAM was placed as a scaffold and Biodentine™ was layered over the HAM with glass ionomer cement and resin composite as coronal seal. Preoperative and post-operative cone beam computed tomography (at three years) was taken to assess the treatment outcome. The resolution of disease process and increase in canal width, as well as positive response to pulp sensitivity tests, were observed by the end of three years. There was approximately 78–86% reduction in the volume of periapical lesion size. This case report confirms that HAM can be used as a scaffold material for successful regenerative endodontic procedure (REP).
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Dawood, Alaa E., David J. Manton, Peter Parashos, and Rebecca H. K. Wong. "The effect of working time on the displacement of Biodentine™ beneath prefabricated stainless steel crown: a laboratory study." Journal of Investigative and Clinical Dentistry 7, no. 4 (May 11, 2015): 391–95. http://dx.doi.org/10.1111/jicd.12162.

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Munoz-Sanchez, Marie-Laure, Natacha Linas, Nicolas Decerle, Emmanuel Nicolas, Martine Hennequin, and Pierre-Yves Cousson. "A Combination of Full Pulpotomy and Chairside CAD/CAM Endocrown to Treat Teeth with Deep Carious Lesions and Pulpitis in a Single Session: A Preliminary Study." International Journal of Environmental Research and Public Health 17, no. 17 (August 31, 2020): 6340. http://dx.doi.org/10.3390/ijerph17176340.

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A higher chance of carrying out a successful full pulpotomy may depend on whether the coronal restoration can be completed within a single appointment. The development of chairside CAD/CAM (Computer Aided Design and Manufacturing) technology has made it possible to carry out indirect restoration of endodontically treated teeth in a single session. This study aimed to evaluate the long-term outcome of a full pulpotomy with Biodentine™ immediately covered with a chairside CAD/CAM endocrown on teeth affected by pulpitis and deep carious lesions. The investigation involved a cohort of 30 molars that were treated by pulpotomy and CAD/CAM endocrown. Clinical and radiological examinations were scheduled at 1, 6, and 12 months postoperatively. Overall, all treatments were effective at any time during the follow-up. The results of this study need to be confirmed with a longer-term follow-up to allow for comparison with the literature. This original combination of endodontic and restorative treatments provides an Endo-prosthetic continuum in a single session, with the objective of long-term success in terms of tooth health.
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Tiwari, Nitika, AnamikaC Borkar, Anita Tandale, Nikhil Nighot, Sailee Ghare, and Sayali Maral. "Comparative evaluation of the effect of various endodontic irrigants on the push-out bond strength of endosequence, Biodentine™, and MTA Plus™ root repair materials: An in vitro study." Journal of the International Clinical Dental Research Organization 11, no. 1 (2019): 9. http://dx.doi.org/10.4103/jicdro.jicdro_3_19.

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Jeon, Jiyoon, Namki Choi, and Seonmi Kim. "Color Change in Tooth Induced by Various Calcium Silicate-Based Pulp-Capping Materials." JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY 48, no. 3 (August 31, 2021): 280–90. http://dx.doi.org/10.5933/jkapd.2021.48.3.280.

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Color stability of pulp-capping material is considered vital to the final aesthetic result since the material is placed in the coronal area. The purpose of this study was to compare the color stability of various pulp-capping materials by analyzing color change of tooth over time. A cavity was formed in the crown of the extracted premolar, and 4 types of pulp-capping materials were filled. Color assessment was performed with a spectrophotometer at different intervals: before placement; immediately after material placement; 1 day, 1 week, 2 weeks, 4 weeks, 8 weeks, 12 weeks, and 16 weeks after placement. Proroot white MTA® and TheraCal LC® showed a significant decrease in the L* value and an increase in the ΔE* value over time. In contrast, Biodentine® and Well-Root™ PT showed no significant change in the L* value and maintained a steady ΔE* value. The application of pulp-capping materials containing bismuth oxide as a radiopacifier may result in a color change of teeth. Long-term color stability of pulp-capping materials should be considered when treating teeth with thin enamel thickness or in aesthetically important area.
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Habashneh, Rola Al, Malik Qabaha, Yousef Khader, Iya Ghassib, and Amre Atmeh. "Gingival Health Around Cervical Carious Lesions Restored with Calcium Silicate-based Cement (Biodentine™) Compared with Glass-ionomer Cement: A Randomized Clinical Trial." Journal of Contemporary Dental Practice 20, no. 6 (2019): 702–6. http://dx.doi.org/10.5005/jp-journals-10024-2583.

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Pawar, AjinkyaMansing, SurajM Pawar, MansingG Pawar, and SharadR Kokate. "Retreatment of endodontically failed tooth with wide-open apex using platelet rich fibrin membrane as matrix and an apical plug of Biodentine™." European Journal of General Dentistry 4, no. 3 (2015): 150. http://dx.doi.org/10.4103/2278-9626.163341.

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42

Gjorgievska, Elizabeta S., John W. Nicholson, Sonja M. Apostolska, Nichola J. Coleman, Samantha E. Booth, Ian J. Slipper, and Mitko I. Mladenov. "Interfacial Properties of Three Different Bioactive Dentine Substitutes." Microscopy and Microanalysis 19, no. 6 (October 22, 2013): 1450–57. http://dx.doi.org/10.1017/s1431927613013573.

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AbstractThree different bioactive materials suitable as dentine substitutes in tooth repair have been studied: glass-ionomer cement, particulate bioglass, and calcium-silicate cement. On 15 permanent human molars, Class V cavities were prepared and the bottom of each cavity was de-mineralized by an artificial caries gel. After the de-mineralization, the teeth were restored with: (1) Bioglass®45S5 and ChemFil® Superior; (2) Biodentine™ and ChemFil® Superior; and (3) ChemFil® Superior for a complete repair. The teeth were stored for 6 weeks in artificial saliva, then cut in half along the longitudinal axis: the first half was imaged in a scanning electron microscope (SEM) and the other half was embedded in resin and analyzed by SEM using energy-dispersive X-ray analysis. The glass-ionomer and the bioglass underwent ion exchange with the surrounding tooth tissue, confirming their bioactivity. However, the particle size of the bioglass meant that cavity adaptation was poor. It is concluded that smaller particle size bioglasses may give more acceptable results. In contrast, both the glass-ionomer and the calcium-silicate cements performed well as dentine substitutes. The glass-ionomer showed ion exchange properties, whereas the calcium silicate gave an excellent seal resulting from its micromechanical attachment.
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Asokan, Sharath, Allwyn Samuel, PR Geetha Priya, and Seby Thomas. "Evaluation of sealing ability of Biodentine™ and mineral trioxide aggregate in primary molars using scanning electron microscope: A randomized controlled in vitro trial." Contemporary Clinical Dentistry 7, no. 3 (2016): 322. http://dx.doi.org/10.4103/0976-237x.188547.

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Martens, L., S. Rajasekharan, and R. Cauwels. "Pulp management after traumatic injuries with a tricalcium silicate-based cement (Biodentine™): a report of two cases, up to 48 months follow-up." European Archives of Paediatric Dentistry 16, no. 6 (May 31, 2015): 491–96. http://dx.doi.org/10.1007/s40368-015-0191-y.

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Sinha, Nidhi, Bijay Singh, and Santosh Patil. "Cone beam-computed topographic evaluation of a central incisor with an open apex and a failed root canal treatment using one-step apexification with Biodentine™: A case report." Journal of Conservative Dentistry 17, no. 3 (2014): 285. http://dx.doi.org/10.4103/0972-0707.131805.

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Bhagwat, SumitaA, Sameeksha Hegde, and LalitagauriP Mandke. "An investigation into the effectiveness of periapical surgery with Biodentine™ used as a root-end filling alone or in combination with demineralized freeze-dried bone allograft and plasma rich fibrin: A 6 months follow-up of 17 cases." Endodontology 28, no. 1 (2016): 11. http://dx.doi.org/10.4103/0970-7212.184324.

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Barczak, Katarzyna, Mirona Palczewska-Komsa, Maciej Sikora, and Jadwiga Buczkowska-Radlińska. "Biodentine™ – use in dentistry. Literature review." Pomeranian Journal of Life Sciences 66, no. 2 (June 17, 2020). http://dx.doi.org/10.21164/pomjlifesci.666.

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Introduction: The concept of “regenerative endodontics”, adopted by the American Association of Endodontics in 2007 was, to an appreciable extent, connected with the introduction of bioactive materials such as Biodentine™ to dental treatment. In the field of biomedical therapeutic materials, the concept of tissue engineering is distinguishable with respect to establishing procedures and materials such as Biodentine™ aimed at replacing injured tissues with those newly developed. The use of Biodentine™ has quickly become a widespread practice in dentistry, commonly used in the biological treatment of pulp and root canal treatment.The aim of this article is a literature review on the use of Biodentine™ in dental practice and its specific characteristics, based on the latest scientific knowledge.Materials and methods: Articles published since the launch of the material into the market were sourced and classifiedaccording to the topic they focussed on. A total of 52 papers were chosen, encompassing those directly focusing on Biodentine™, as well as other relevant papers not mentioning Biodentine™, but pertaining to dental materials in general.Conclusion: On the grounds of this analysis of literature on the subject, it can be concluded that bioactive materials such as Biodentine™ have a wide range of application in therapeutic dental procedures. There are numerous studies presenting the advantages of this group of materials. However, the use of Biodentine™ still requires a comprehensive and long-term analysis to unequivocally confirm its therapeutic success following use of this material.Keywords: direct pulp capping; pulpotomy; root perforation; invasive cervical root resorption; bioceramics; apexification; regenerative endodontic procedures.
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Manaspon, Chawan, Chavin Jongwannasiri, Sujin Chumprasert, Noppadol Sa-Ard-Iam, Rangsini Mahanonda, Prasit Pavasant, Thantrira Porntaveetus, and Thanaphum Osathanon. "Human dental pulp stem cell responses to different dental pulp capping materials." BMC Oral Health 21, no. 1 (April 26, 2021). http://dx.doi.org/10.1186/s12903-021-01544-w.

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Abstract Background Direct pulp capping is a vital pulp therapy for a pin-point dental pulp exposure. Applying a pulp capping material leads to the formation of a dentin bridge and protects pulp vitality. The aim of this study was to compare the effects of four dental materials, DyCal®, ProRoot® MTA, Biodentine™, and TheraCal™ LC in vitro. Methods Human dental pulp stem cells (hDPs) were isolated and characterized. Extraction medium was prepared from the different pulp capping materials. The hDP cytotoxicity, proliferation, and migration were examined. The odonto/osteogenic differentiation was determined by alkaline phosphatase, Von Kossa, and alizarin red s staining. Osteogenic marker gene expression was evaluated using real-time polymerase chain reaction. Results ProRoot® MTA and Biodentine™ generated less cytotoxicity than DyCal® and TheraCal™ LC, which were highly toxic. The hDPs proliferated when cultured with the ProRoot® MTA and Biodentine™ extraction media. The ProRoot® MTA and Biodentine™ extraction medium induced greater cell attachment and spreading. Moreover, the hDPs cultured in the ProRoot® MTA or Biodentine™ extraction medium migrated in a similar manner to those in serum-free medium, while a marked reduction in cell migration was observed in the cells cultured in DyCal® and TheraCal™ LC extraction media. Improved mineralization was detected in hDPs maintained in ProRoot® MTA or Biodentine™ extraction medium compared with those in serum-free medium. Conclusion This study demonstrates the favorable in vitro biocompatibility and bioactive properties of ProRoot® MTA and Biodentine™ on hDPs, suggesting their superior regenerative potential compared with DyCal® and TheraCal™.
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Garrido, Mauricio, Diego Morales, María Paz Saldías, Christian Fernández, Veronica Villalobos, Oscar Cerda, and Mónica Cáceres. "Cellular response of human apical papilla cells to calcium hydroxide and tricalcium silicate-based cements." BMC Oral Health 21, no. 1 (March 9, 2021). http://dx.doi.org/10.1186/s12903-021-01467-6.

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Abstract Background This study aimed to evaluate the biological response of human apical papilla cells to different calcium hydroxide formulations and three tricalcium silicate-based materials. Methods Primary cells were obtained from explants of young immature premolars. 20,000 cells adhered for 24 h over discs of Biodentine™, ProRoot®MTA, BioRoot®RCS and calcium hydroxide mixed either with sodium chloride 0.9%w/v or polyethylene glycol and UltraCal® were used to evaluate cell adhesion by scanning electron microscopy and cell viability by MTT assay. Results Cells adhered to ProRoot®MTA showed an increase of F-actin like protrusions, suggesting bioactivity. Cells adhered to UltraCal® show protrusion such as filopodia. On the contrary, cells adhered to BioRoot®RCS showed no signs of any cellular protrusion. Regarding viability between the materials, we found a higher percentage of viability in cells cultured over discs of Biodentine™ and ProRoot®MTA. Conclusion ProRoot®MTA and Biodentine™ exhibit a better cellular response of human apical papilla cells in vitro conditions compared to BioRoot® and calcium hydroxide diluted in sodium chloride.
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"SPE#16 – Aplicações Clínicas de Biodentine™: série de casos." Revista Portuguesa de Estomatologia, Medicina Dentária e Cirurgia Maxilofacial 60, s1 (December 18, 2019). http://dx.doi.org/10.24873/j.rpemd.2019.12.657.

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