Academic literature on the topic 'Biolabel'

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Journal articles on the topic "Biolabel"

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Qin, Xiaoli, Aigui Xu, Ling Liu, Wenfang Deng, Chao Chen, Yueming Tan, Yingchun Fu, Qingji Xie, and Shouzhuo Yao. "Ultrasensitive electrochemical immunoassay of proteins based on in situ duple amplification of gold nanoparticle biolabel signals." Chemical Communications 51, no. 40 (2015): 8540–43. http://dx.doi.org/10.1039/c5cc01439e.

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An electrochemical sandwich immunoassay method that can be sensitive to a few protein molecules (human immunoglobulin G or human prostate-specific antigen) is reported based on in situ duple amplification of gold nanoparticle biolabel signals.
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Oviedo, M. J., O. E. Contreras, Y. Rosenstein, R. Vazquez-Duhalt, Z. S. Macedo, G. G. Carbajal-Arizaga, and G. A. Hirata. "New Bismuth Germanate Oxide Nanoparticle Material for Biolabel Applications in Medicine." Journal of Nanomaterials 2016 (2016): 1–10. http://dx.doi.org/10.1155/2016/9782625.

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Bismuth germanate (Bi4Ge3O12, BGO) has been the focus of several studies due to its scintillation properties. It has been employed as detector in scientific research and medicine, and herein we studied its possible biomedical applications. The photoluminescence properties of the uncoated and protein-coated nanoparticles were analyzed in different body fluids, at different pH. The nanoparticles yielded blueish-white luminescence with a maximum emission peak at 485 nm corresponding to the3P1→1S0electron transition of Bi3+. They showed luminescence properties at different pH values and in human fluids, such as urine and blood serum. Finally, the BGO nanoparticles were functionalized with the anti-HLA I W6/32 monoclonal antibody and the capacity of the antibody-loaded nanoparticles to recognize the cognate antigen (HLA I) of the W6/32 mAb was assessed on the human promyelocytic leukemia cell line THP-1. The possibility of functionalizing BGO nanoparticles with W6/32 antibodies and their specificity to identify THP-1 cells make them promising candidates for biomedical applications as biolabels.
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Bi, Sai, Hong Zhou, and Shusheng Zhang. "Multilayers enzyme-coated carbon nanotubes as biolabel for ultrasensitive chemiluminescence immunoassay of cancer biomarker." Biosensors and Bioelectronics 24, no. 10 (June 2009): 2961–66. http://dx.doi.org/10.1016/j.bios.2009.03.002.

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Sun, Ai-Li, Feng-Chun Jia, Yan-Fang Zhang, and Xuan-Nian Wang. "Gold nanocluster-encapsulated glucoamylase as a biolabel for sensitive detection of thrombin with glucometer readout." Microchimica Acta 182, no. 5-6 (December 12, 2014): 1169–75. http://dx.doi.org/10.1007/s00604-014-1440-1.

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Zhang, Hui, Lu Tian, Ruoyu Zhang, Zhiqiang Ye, and Jingli Yuan. "Preparation of visible-light-excited luminescence enhancement solutions for time-resolved luminescence detection of europium biolabel." Analyst 137, no. 19 (2012): 4502. http://dx.doi.org/10.1039/c2an35719d.

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Gouvêa, A., A. T. Pereira, A. C. Pimentel, D. M. F. Prazeres, V. Chu, and J. P. Conde. "Colorimetric detection of molecular recognition reactions with an enzyme biolabel using a thin-film amorphous silicon photodiode on a glass substrate." Sensors and Actuators B: Chemical 135, no. 1 (December 2008): 102–7. http://dx.doi.org/10.1016/j.snb.2008.07.030.

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Li, Xu-Zhao, Hua-Jin Huang, Shuai-Nan Zhang, Qi Liu, and Yu-Mei Wang. "Label-free quantitative proteomics positions the effects and mechanisms of Herba Lysimachiae on synovial diseases based on biolabel-led research pattern." Journal of Chromatography B 1138 (February 2020): 121969. http://dx.doi.org/10.1016/j.jchromb.2020.121969.

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Qin, Xiaoli, Aigui Xu, Ling Liu, Yuyun Sui, Yunlong Li, Yueming Tan, Chao Chen, and Qingji Xie. "Selective staining of CdS on ZnO biolabel for ultrasensitive sandwich-type amperometric immunoassay of human heart-type fatty-acid-binding protein and immunoglobulin G." Biosensors and Bioelectronics 91 (May 2017): 321–27. http://dx.doi.org/10.1016/j.bios.2016.12.051.

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LI, Xu-zhao, Hong-mei LI, Shuai-nan ZHANG, Qi LIU, and Yu-mei WANG. "A biolabel research based on metabonomics reveals the therapeutic potentials of Herba Lysimachiae in synovial diseases: The dual effects on synovial platelet aggregation by prostaglandin E1/E2." Journal of Chromatography B 1174 (June 2021): 122726. http://dx.doi.org/10.1016/j.jchromb.2021.122726.

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Eriksson, E., J. Lysell, H. Larsson, K. Y. Cheung, D. Filippini, and W. C. Mak. "Geometric Flow Control Lateral Flow Immunoassay Devices (GFC-LFIDs): A New Dimension to Enhance Analytical Performance." Research 2019 (June 17, 2019): 1–8. http://dx.doi.org/10.34133/2019/8079561.

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The nitrocellulose (NC) membrane based lateral flow immunoassay device (LFID) is one of the most important and widely used biosensor platforms for point-of-care (PoC) diagnostics. However, the analytical performance of LFID has limitations and its optimization is restricted to the bioassay chemistry, the membrane porosity, and the choice of biolabel system. These bottom neck technical issues resulted from the fact that the conventional LFID design principle has not evolved for many years, which limited the LFID for advanced biosensor applications. Here we introduce a new dimension for LFID design and optimization based on geometric flow control (GFC) of NC membranes, leading to highly sensitive GFC-LFID. This novel approach enables comprehensive flow control via different membrane geometric features such as the width (w) and the length (l) of a constriction, as well as its input angle (θ1) and output angle (θ2). The GFC-LFID (w=0.5 mm, l=7 mm, θ1= 60°, θ2= 45°) attained a 10-fold increase in sensitivity for detection of interleukin-6 (IL-6), compared with conventional LFID, whereas reducing by 10-fold the antibody consumption. The GFC-LFID detects IL-6 over a linear range of 0.1–10 ng/mL with a limit of detection (LoD) of 29 pg/mL, which even outperforms some commercial IL-6 LFIDs. Such significant improvement is attained by pure geometric control of the NC membrane, without additives, that only relaying on a simple high throughput laser ablation procedure suitable for integration on regular large-scale manufacturing of GFC-LFIDs. Our new development on GFC-LFID with the combination of facile scalable fabrication process, tailored flow control, improved analytical performance, and reduced antibodies consumption is likely to have a significant impact on new design concept for the LFID industry.
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Dissertations / Theses on the topic "Biolabel"

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Janczak, Colleen. "Hybrid Nanoparticles for Enhanced Sensitivity in Biological Labeling and Biomolecular Sensing." Diss., The University of Arizona, 2011. http://hdl.handle.net/10150/202514.

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Nanoparticles (nPs) demonstrate significant advantages over other sensor and marker technologies. The most useful optical nanosensor and label platform for biological samples would be non-toxic, hydrophilic, resistant to non-specific protein interactions and degradation over time or under harsh conditions, highly retentive of entrapped components, and easily functionalized for target specificity. The work described here is part of an investigation into the fabrication and application of polyacrylamide, polyacrylamide/silica hybrid, and polystyrene-core silica-shell nPs. Polyacrylamide (PA) nP nitric oxide (NO) sensors were made by co-entrapping 4, 5-diaminofluorescein (DAF-2) and Texas Red dextran in 60 nm PAnPs. Sensors were used to measure NO produced by a diazeniumdiolate NO donor in solution, and have a response time of 30 seconds or less. Entrapped DAF-2 was protected from non-specific interactions with bovine serum albumin (BSA). Sensor response to NO in FBS solutions was reduced compared to buffer, although improvement over free dyes was observed. The sensors were applied to J477A.1 macrophages as well as a HT1080 cell line (HTRiNOS) in preliminary studies for measuring intracellular NO production. Polyacrylamide/silica hybrid nPs were fabricated and nP architecture was evaluated by transmission electron microscopy. Isopycnic centrifugation of nP samples indicates that the hybrid nPs have a density between 1.70 and 1.76 g/cm³. Silica in the hybrid nPs was covalently labeled with Texas Red, suggesting that the hybrid nPs may be used as ratiometric or possibly multiplexed sensors. Hybrid nPs coated with 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) exhibit reduced adsorption of TRITC-BSA compared to uncoated hybrid nPs. Hybrid nP pH sensors were prepared and responded reproducibly and reversibly to changes in pH, nominally from pH 6.0 to 8.0. Core-shell nPs for scintillation proximity assay (SPA) were fabricated by entrapping the scintillants p-terphenyl and 4-bis(4-methyl-5-phenyl-2oxyzolyl)benzene in polystyrene, onto which silica shells were subsequently added. Core-shell nPs were found to have a scintillation response similar to that of shell-less polystyrene cores, indicating that the presence of the silica shells does not reduce scintillation efficiency. Preliminary studies using core-shell nPS for biotin-streptavidin binding SPA do not indicate an enhancement in scintillation efficiency, although this may be due to high nP:radiolabeled analyte ratios.
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Feng, Ke. "Biolayer modeling and optimization for the SPARROW biosensor." Morgantown, W. Va. : [West Virginia University Libraries], 2007. https://eidr.wvu.edu/etd/documentdata.eTD?documentid=5235.

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Thesis (Ph. D.)--West Virginia University, 2007.
Title from document title page. Document formatted into pages; contains v, 137 p. : ill. (some col.). Includes abstract. Includes bibliographical references (p. 126-137).
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Henderson, Andrew P. "Impedance analysis and mathematical modelling of immunosensor biolayer." Thesis, Teesside University, 2011. http://hdl.handle.net/10149/333365.

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A study to optimise an IgG based immunosensor is presented, that has been carried out by absorbing monolayers to a gold transducer surface at varying immersion times and temperatures. The theory and kinetics of monolayer adsorption are analysed and discussed. Existing mathematical models are reviewed and experimentally researched, to highlight gaps in knowledge that would facilitate high quality, cost effective immunosensor production. The creation of two mathematical models to predict monolayer adsorption kinetics and optimal immersion times are discussed. Details are provided of how the new mathematical models may be advanced, and how the production of immunosensors may be further improved. The first novel mathematical model (PTCS) has been created to model the presence of two sequentially forming structures on the surface of a substrate. It gives an insight into the percentages of each structure on the surface, along with the actual adsorption process. This model provides a good fit to all applicable experimental data and has allowed the deduction of optimum immersion times. The second novel model (PIF) provides a greater insight than existing models into the individual contributions to surface coverage by both random and island growth. This allows an insight into how the monolayer surface is covered, which is critical to determine the optimum conditions for adsorption. This model also provides a good fit to the isotherm data it has been applied to. To provide a thorough understanding of the bulk properties of monolayer formation over the gold transducer, and how these properties vary with immersion time and temperature, various measurement techniques have been employed. Electrochemical Impedance Spectroscopy (EIS) has been the principle measurement technique used to measure the bulk properties, but confirmation studies have also been carried out including, Contact angle measurements, FTIR microscopy with BSA molecular labels, Fluorescence microscopy for small adsorbed molecules and AFM for layers assembled from macromolecules. The data generated from the different techniques show consistency with the arguments discussed in each instance. Two different IgG adsorption processes have been compared. These include direct IgG addition and a multilayered streptavidin-based process. The results indicate that IgG molecules adsorbed via the streptavidin based multilayer process are more vertically orientated and have a higher packing density of IgG molecules. Keywords: Self Assembled Monolayer, impedance-based immunoassay, Streptavidin, biotinylated IgG, mathematical adsorption modelling.
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Hummerhielm, Linda. "Biolayer development in a slow sand filter in Ghana : Designing a filter that is benefiting the biolayer development under local conditions." Thesis, Karlstads universitet, Fakulteten för hälsa, natur- och teknikvetenskap (from 2013), 2017. http://urn.kb.se/resolve?urn=urn:nbn:se:kau:diva-62601.

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In 2015, the United nations presented the 17 Global Goals that would put an end to extreme poverty, inequality and climate change by 2030. One of these goals was clean water and sanitation. In 2015 1.8 billion people did not have access to clean water. Because of the contaminated water, one million people die every year worldwide. Africa, and especially Ghana, has had a high development in the recent years. The population has grown and more resources are needed. Clean water in Ghana is not a given matter, three million people live without access to clean water. To work towards the Global Goal water can be clean locally. A simple and cheap way is to build slow sand filters, which also are the purpose of this project. These filters purify the water mechanically, chemically and biologically. The biologically purification takes place in the biolayer that grows on the sand inside the filter and it consumes contaminants in the water. It takes about a month for the biolayer to be fully developed and clean the water to its full potential. The positive aspects with sand filters are that people get healthier and can save money that can be invested in education or business. It can also reduce the need for water in plastic bags or bottles and would reduce littering. The companies that produce this water could end their business and air pollutions would be reduced as well.   During this project, slow sand filters have been tested and evaluated in Sweden and Ghana with the purpose to develop a theoretical filter that benefits the biolayer under local conditions in Ghana, this was of the one aims. Experiments in Sweden showed that the flow decreased with increased sand height and decreased hydraulic head. In Ghana three filters were built with the sand heights 30, 50 and 80 cm to clean 7 litres of drinking water for a family of four. None of these produced drinkable water by WHO’s and EU’s standards.   The next aim was to understand which chemical and physical factors that effected the development of the biolayer. The detected relations were absolute conductivity, total alkalinity, coliform bacteria and oxidantial reduction potential which were between the biolayer in the 30 and 50 filters.   The flow rate in Ghana was too high and to lower it, a new diffuser with smaller holes would be built to get the recommended flow of 0,4 m3/m2/h. A too high flow broke the bound between the biolayer and made an uncomfortable environment. A sedimentation should be installed before the sand filter to reduce the variations of the incoming water such as turbidity, suspended solids etc., so the biolayer would flourish. It was not enough dissolved oxygen in the water so the pause period would be decreased to 12 hours to get more oxygen in the filter each day. For a sand filter to work as planned a lot of attention should be given to the filter. It is a system that should be used all the time for the best purification. To build a filter takes a lot of time and it also takes time for the biolayer to develop. If it is not going to be used much, another treatment method should be used.   The last aim was to evaluate the cost of the materials that could be bought locally to the filter. One filter cost about 130 GHS.
2015 tog Förenta nationerna fram de 17 globala målen för att få ett slut på extrem fattigdom, ojämlikhet och klimatförändringen till år 2030. Ett av dessa mål handlar om rent vatten och sanitet. 2015 var det 1,8 miljarder människor som inte hade tillgång till rent vatten. På grund av det förorenade vattnet dör en miljon människor i hela världen varje år. Afrika, och speciellt Ghana, har haft en snabb utveckling de senaste åren. Folkmängden har ökat och mer naturresurser behövs. Rent vatten i Ghana är inte en självklarhet, tre miljoner människor lever idag utan tillgång till rent vatten i Ghana. Ett sätt för att jobba mot det globala målet är rening av vatten lokalt. Ett enkelt och billigt sätt är att bygga långsamsandfilter, vilket även var syftet med denna studien. Dessa filter renar vattnet mekaniskt, kemiskt och biologiskt. Den biologiska reningen sker av en biofilm som växer på sanden inuti filtret som konsumerar föroreningar i vattnet. Det tar ungefär en månad för biofilmen att bli färdigutvecklad och rena vattnet till sin fulla potential. Det positiva med sandfilter är att människorna skulle bli friskare och spara pengar som kan investeras på utbildning eller företag. Ur miljöpunkt skulle reduktionen av köpt vatten i plastpåsar och flaskor minska nedskräpningen och företagen som producerar dessa kan avsluta produktionen och därmed minska luftföroreningar.    Under detta projekt har långsamsandfilter utvärderats både i Sverige och Ghana för att utveckla ett nytt teoretiskt filter som gynnar tillväxten av biofilm under lokala förhållanden i Ghana, vilket var ett mål. Experimenten i Sverige visade att flödet sjönk med ökad sandhöjd, men även med minskat hydrauliskt tryck. I Ghana byggdes tre filter med sand höjderna 30, 50 och 80 cm för att rena 7 liter dricksvatten till en familj på fyra. Ingen av dessa lyckades producera drickbart vatten enligt WHO:s och EU:s standarder.   Nästa mål var att förstå vilka av de kemiska och fysiska faktorer som påverkade biofilmstillväxten. Det förhållanden som upptäcktes var absolut konduktivitet, total alkalinitet, coliform bacteria och oxidential reduction potential vilket fanns i 30 och 50 filtret.   Flödet i Ghana var för högt, så för att minska det skulle en diffusör med mindre hål byggas för att få det rekommenderade flödet 0,4 m3/m2/h. Ett för högt flöde gjorde sönder bindingen mellan biofilmen och skapade en otrivsam miljö. En sedimentation skulle installeras innan sandfiltret för att minska variationer på ingående vatten i filtret för att få biofilmen att trivas bättre. Det fanns för lite löst syre i vattnet och om pausperioden minskas till 12 timmar skulle mer syre i filtret varje dag. För att ett sandfilter ska fungera som planerat måste mycket tid läggas på filtret. Sandfilter är ett system som bör används ofta för bästa rening. Att bygga ett filter kräver mycket tid, samt att det tar tid innan biofilmen har utvecklats. Om sandfiltret inte kommer används mycket föreslås att en annan metod används istället.   Det sista målet var att utvärdera kostnaden av materialen som kunde köpas lokalt till filtret. Ett filter kostade runt 130 GHS.
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Höfelschweiger, Bianca K. "The pyrylium dyes a new class of biolabels ; synthesis, spectroscopy, and application as labels and in general protein assay /." [S.l. : s.n.], 2005. http://deposit.ddb.de/cgi-bin/dokserv?idn=975903071.

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Biscans, Annabelle. "Synthèse et évaluation d’oligoribonucléotides 2’-O-modifiés par des groupements biolabiles acétalesters ou alkyldithiométhyles dans une approche de prodrogues d’ARN interférents." Thesis, Montpellier, 2015. http://www.theses.fr/2015MONTS020.

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Les ARN interférents sont de puissants outils thérapeutiques et biologiques pour la mise en silence de l'expression des gènes. Afin d'améliorer leur stabilité enzymatique, leur biodistribution et leur pénétration cellulaire, nous proposons de développer une approche prodrogue d'ARN interférent. Ce manuscrit rapporte la synthèse et l'évaluation de pro-ARN masqués temporairement par des groupements biolabiles susceptibles d'être hydrolysés dans les cellules afin de libérer l'ARN naturel actif. Deux types de modifications sont présentés : des groupes acétalesters enlevés par des carboxyestérases et des groupes alkyldithiométhyles sensibles à un environnement réducteur. Dans une première partie, une nouvelle méthode de synthèse de pro-ARN partiellement modifiés en position 2' par des groupements acétalesters est décrite. Plusieurs groupements variant par leur caractère lipophile ou cationique sont évalués. Des résultats prometteurs d'études physico-chimiques, de stabilité enzymatique, de pénétration cellulaire et d'inhibition de gènes mettent en valeur l'intérêt d'utiliser certains pro-ARN modifiés en tant qu'outils thérapeutiques. Une deuxième partie présente une voie de synthèse originale de pro-ARN modifiés en position 2' par des groupements alkyldithiométyles. Les propriétés physico-chimiques, la stabilité enzymatique et le démasquage de ces pro-ARN sont décrits. Parallèlement, l'étude d'une réaction d'échange thiol-disulfure permettant l'incorporation de liens disulfures intrabrin au sein de duplex d'ARN et de constructions tige-boucles est détaillée dans ce manuscrit
SiRNA are powerful therapeutic and biological tools for gene silencing. In the aim of improving their stability, their biodistribution and their cellular delivery, we propose to develop a siRNA prodrug-like approach.This manuscript reports the synthesis and the study of pro-RNA temporarily masked by biolabile groups which could be hydrolyzed inside cells in order to release the active unmodified RNA. Two types of modifications are presented: acetalester groups removed by carboxyesterases and alkyldithiomethyl groups cleaved in a reducing environment within cells.In a first part, a new synthesis strategy of partially modified 2'-O-acetalester pro-RNA is described. Several acetalester groups varying in their lipophilicity and their charge are evaluated. Promising results obtained in physical-chemical studies, enzymatic stability and gene inhibition highlight the use of these modified pro-RNA as therapeutic drugs. A second part introduces an original approach for the synthesis of 2'-O-alkyldithiomethyl pro-RNA. The physical-chemical properties, the enzymatic stability and the unmasking of this pro-RNA are described.Moreover, the study of a thiol-disulfide exchange reaction allowing the incorporation of intrastrand disulfide bond into secondary structure duplex and hairpin is reported in this manuscript
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Horn, Astrid Verfasser], and Karl-Heinrich [Akademischer Betreuer] [Grote. "Vorbereitungen für das Biolab Experiment TRIPLE LUX A : Hardwareentwicklung, Kalibrierung und biologische Bodenkontrollen / Astrid Horn. Betreuer: Karl-Heinrich Grote." Magdeburg : Universitätsbibliothek, 2011. http://d-nb.info/1047561913/34.

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Spadanuda, Antonio. "Evolution of the structural and fracture design of the ISS payloads due to the new launchers, specifically applied to Biolab IEC-2 projects." Master's thesis, Alma Mater Studiorum - Università di Bologna, 2014. http://amslaurea.unibo.it/6810/.

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The relatively young discipline of astronautics represents one of the scientifically most fascinating and technologically advanced achievements of our time. The human exploration in space does not offer only extraordinary research possibilities but also demands high requirements from man and technology. The space environment provides a lot of attractive experimental tools towards the understanding of fundamental mechanism in natural sciences. It has been shown that especially reduced gravity and elevated radiation, two distinctive factors in space, influence the behavior of biological systems significantly. For this reason one of the key objectives on board of an earth orbiting laboratory is the research in the field of life sciences, covering the broad range from botany, human physiology and crew health up to biotechnology. The Columbus Module is the only European low gravity platform that allows researchers to perform ambitious experiments in a continuous time frame up to several months. Biolab is part of the initial outfitting of the Columbus Laboratory; it is a multi-user facility supporting research in the field of biology, e.g. effect of microgravity and space radiation on cell cultures, micro-organisms, small plants and small invertebrates. The Biolab IEC are projects designed to work in the automatic part of Biolab. In this moment in the TO-53 department of Airbus Defence & Space (formerly Astrium) there are two experiments that are in phase C/D of the development and they are the subject of this thesis: CELLRAD and CYTOSKELETON. They will be launched in soft configuration, that means packed inside a block of foam that has the task to reduce the launch loads on the payload. Until 10 years ago the payloads which were launched in soft configuration were supposed to be structural safe by themselves and a specific structural analysis could be waived on them; with the opening of the launchers market to private companies (that are not under the direct control of the international space agencies), the requirements on the verifications of payloads are changed and they have become much more conservative. In 2012 a new random environment has been introduced due to the new Space-X launch specification that results to be particularly challenging for the soft launched payloads. The last ESA specification requires to perform structural analysis on the payload for combined loads (random vibration, quasi-steady acceleration and pressure). The aim of this thesis is to create FEM models able to reproduce the launch configuration and to verify that all the margins of safety are positive and to show how they change because of the new Space-X random environment. In case the results are negative, improved design solution are implemented. Based on the FEM result a study of the joins has been carried out and, when needed, a crack growth analysis has been performed.
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Frausto, Harissa Silvério El Ghoz. "Avaliação da eficiência dos métodos VIDAS® Biolab-Mérieux e Bax® (Dupont) na detecção de Salmonella spp. em carne suína, bovina e de frango." Universidade Estadual de Londrina. Centro de Ciências Agrárias. Programa de Pós-Graduação em Ciência de Alimentos, 2011. http://www.bibliotecadigital.uel.br/document/?code=vtls000183649.

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A presença de Salmonella ssp torna os alimentos impróprios para consumo humano, sendo necessário métodos de detecção confiáveis para a salmonelose, nos laboratórios de controle de qualidade e de diagnóstico. A detecção de Salmonella spp. em alimentos pelo método tradicional é demorada, o que explica a abundância de sistemas automatizados e kits disponíveis comercialmente para detecção rápida deste patógeno. Muitos desses métodos alternativos são validados por organizações internacionalmente reconhecidas, mas podem apresentar resultados diferentes quando utilizados com alimentos naturalmente contaminados. Principalmente pelo fato de que a maioria das validações é realizada com amostras contaminadas artificialmente, não representando a realidade da microbiota normal das amostras. O objetivo deste trabalho é a cavaliação dos métodos rápidos alternativos VIDAS® Salmonella (SLM), BAX® System e método tradicional (IN 62, MAPA) na detecção de Salmonella spp. em alimentos. Os métodos foram testados com amostras naturalmente contaminadas de carne de frango, suína e bovina. O método tradicional detectou 20 amostras (2,1 %) positivas para Salmonella spp. enquanto o método VIDAS® encontrou 87 amostras positivas (9,2 %), demonstrando uma especificidade de 93,0 %. No método BAX® foram 741 (12,7 %) resultados positivos para Salmonella spp. sendo que a metodologia tradicional detectou 230 (4,0 %) amostras positivas indicando 90,5 % de especificidade. Do total de 221 amostras de Salmonella spp. sorotipadas, o sorovar mais freqüente foi S. Minnesota (n=28; 15,7 %), seguida de S. Mbandaka (n=17; 9,5 %), S. Schwarzengrund (n=15; 8,4 %), S. Saintpaul (n=14; 7,8 %) e S. Enteritidis (n=13; 7,3 %). A diminuição no isolamento de S. Enteritidis (SE) pode ser uma conseqüência do Programa Nacional de Redução de Patógenos implantado pelo Ministério da Agricultura, Pecuária e Abastecimento, em 2003 e também pela vacinação para SE das matrizes de corte. Não foi possível saber com segurança se essa diminuição no isolamento de SE levou a um impacto positivo na saúde pública. É muito importante o contínuo monitoramento e a melhoria do diagnóstico em casos esporádicos e de surtos de infecção humana. Embora a freqüência do isolamento de S. Minnesota tenha aumentado, esse sorovar não foi associado a casos esporádicos e surtos de salmonelose ocorridos em 2009 e 2010 no Paraná. No entanto, é essencial que as autoridades sanitárias avaliem a repercussão na saúde Pública do aumento do isolamento deste sorovar em carne de frango.
The presence of Salmonella ssp makes food unfit for human consumption, requiring reliable detection methods for Salmonella in laboratories for quality control and diagnostics. The detection of Salmonella spp. in food by the traditional method is time consuming, which explains the abundance of automated systems and commercially available kits for rapid detection of this pathogen. Many of these alternative methods are validated by internationally recognized organizations, but may have different results when used with naturally contaminated food. Especially the fact that most of the validations is performed with artificially contaminated samples do not represent the reality of the normal microbiota of the samples. The objective of this work is the quick alternative methods cavaliação VIDAS ® Salmonella (SLM), BAX ® System and the traditional method (62 IN, MAP) in the detection of Salmonella spp. in foods. The methods were tested with naturally contaminated samples of chicken, pork and beef. The traditional method detected 20 samples (2.1%) tested positive for Salmonella spp. VIDAS ® while the method found 87 positive samples (9.2%), demonstrating a specificity of 93.0%. In the BAX ® method were 741 (12.7%) tested positive for Salmonella spp. being that the traditional method detected 230 (4.0%) positive samples indicating 90.5% specificity. Of the total 221 samples of Salmonella spp. serotyped, the most frequent serovar was S.Minnesota (n = 28, 15.7%), followed by S. Mbandaka (n = 17, 9.5%), S. Schwarzengrund (n = 15, 8.4%), S. Saintpaul (n = 14, 7.8%) and S. Enteritidis (n = 13, 7.3%). The decrease in the isolation of S. Enteritidis (SE) may be a consequence of the National Pathogen Reduction implemented by the Ministry of Agriculture, Livestock and Supply, in 2003 and also for SE by vaccination of cutting dies. It was not possible to know with certainty whether this decrease in the isolation of SE led to a positive impact on public health. It is very important to the continuous monitoring and improvement of diagnosis in sporadic cases and outbreaks of human infection. Although the frequency of isolation of S. Minnesota has increased, this serovar was not associated with sporadic cases and outbreaks of salmonellosis occurred in 2009 and 2010 in Paraná. However, it is essential that health officials to assess the public health impact of increased isolation of this serovar in poultry meat.
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Septiadi, Dedy. "Optical imaging and drug delivery using soft- and hard- nanomaterials." Thesis, Strasbourg, 2015. http://www.theses.fr/2015STRAF036/document.

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Le travail décrit dans cette thèse se concentre sur le développement de matériaux « durs et mous » ainsi que leur interaction avec les cellules biologiques pour une application finale dans le domaine de la théranostique couvrant l'imagerie, la détection, la thérapie génique et la thérapie du cancer. Dans ce contexte, nous avons tout d'abord étudié l'utilisation de complexes (II) de platine phosphorescents auto-assemblés comme sonde cellulaire. Nous avons étendu l'idée de bio-imagerie en introduisant un concept d’imagerie basée sur l’émission stimulée où nous étions en mesure de générer un laser provenant d'une cellule biologique unique sans utiliser de cavité optique conventionnelle. En outre, des nano-transporteurs multifonctionnels à base de matières poreuses dures à savoir des zéolithes L et des nanoparticules de silice mésoporeuse pour de la « drug delivery » (relargage de médicaments et d’oligonucléotides) in vitro ide ont été développés avec succès et testés pour le traitement du glioblastome. Un autre nano-vecteur, qui est construit à partir de silice biodégradable, a également été synthétisé et sa capacité d'encapsuler des protéines et de les libérer dans les cellules vivantes lors de la dégradation de la structure dans un environnement réducteur a été démontrée. Enfin, l'utilisation de nouveaux matériaux plasmonique sur la base de nanoparticules d'argent enrobées de silice cassable pour la détection d'agents réducteurs a été mise en valeur
The work described in this thesis focuses on the development of soft- and hard-materials as well as their interaction with biological cells for applications in the field of theranostics covering imaging, sensing, and gene, and cancer therapy. In this context, we first investigated the use of phosphorescent self-assembled platinum(II) complexes as cellular probes. We extended the concept stimulated emission-based bioimaging by generating a laser-like emission coming from a single biological cell without using any conventional optical cavity. In addition, we successfully developed multifunctional nanocarriers based on porous hard materials, namely zeolites-L and mesoporous silica nanoparticles for drug and oligonucleotide delivery in vitro and they were tested to treat glioblastoma. Another nanovector, which is constructed from biodegradable silica, was also synthesized and its ability to encapsulate proteins and release them in living cells upon degradation of the structure in reductive environment was demonstrated. Finally, the use of novel plasmonic structures based on breakable silica-coated silver nanoparticles for detection of reducing agents was successfully investigated
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Books on the topic "Biolabel"

1

Pentz, Lundy Hurd. The biolab book. 2nd ed. Baltimore: Johns Hopkins University Press, 1989.

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New England Biolabs Workshop on Biological DNA Modification (1988 Gloucester, Mass.). Papers presented at the New England Biolabs Workshop on Biological DNA Modification, Twin Light Manor, Gloucester, MA (U.S.A.), 20-23 May 1988. Amsterdam: Elsevier Science Publishers, 1988.

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The Biolab Book: Laboratory Studies in Life. 2nd ed. Biomateria Publishing Company, Incorporated, 1985.

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Zachry, Joel G. Biolab: A Laboratory Notebook for General Biology. Kendall/Hunt Publishing Company, 1992.

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Wilson, Stephen. Biolab: A Laboratory Manual for Biology and Ecology. Kendall/Hunt Publishing Company, 1991.

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Biolab and Minilab Worksheets (Biology The Dynamics of Life). Glencoe MacMillan McGraw Hill, 1995.

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Ltd, ICON Group. BIOLASE TECHNOLOGY, INC.: Labor Productivity Benchmarks and International Gap Analysis (Labor Productivity Series). 2nd ed. Icon Group International, 2000.

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Ltd, ICON Group. BIOLASE TECHNOLOGY, INC.: International Competitive Benchmarks and Financial Gap Analysis (Financial Performance Series). 2nd ed. Icon Group International, 2000.

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Book chapters on the topic "Biolabel"

1

Tanev, Georgi, Winnie Svendsen, and Jan Madsen. "Biolabs as Computing Components." In Embedded, Cyber-Physical, and IoT Systems, 263–82. Cham: Springer International Publishing, 2019. http://dx.doi.org/10.1007/978-3-030-16949-7_12.

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Martin, Stephen R., Andres Ramos, and Laura Masino. "Biolayer Interferometry: Protein–RNA Interactions." In Protein-Ligand Interactions, 351–68. New York, NY: Springer US, 2021. http://dx.doi.org/10.1007/978-1-0716-1197-5_16.

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Farias, Patricia M. A., Beate S. Santos, and Adriana Fontes. "Semiconductor Fluorescent Quantum Dots: Efficient Biolabels in Cancer Diagnostics." In Micro and Nano Technologies in Bioanalysis, 407–19. Totowa, NJ: Humana Press, 2009. http://dx.doi.org/10.1007/978-1-59745-483-4_27.

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Kumaraswamy, Sriram, and Renee Tobias. "Label-Free Kinetic Analysis of an Antibody–Antigen Interaction Using Biolayer Interferometry." In Methods in Molecular Biology, 165–82. New York, NY: Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2425-7_10.

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Ciesielski, Grzegorz L., Vesa P. Hytönen, and Laurie S. Kaguni. "Biolayer Interferometry: A Novel Method to Elucidate Protein–Protein and Protein–DNA Interactions in the Mitochondrial DNA Replisome." In Methods in Molecular Biology, 223–31. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-3040-1_17.

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Clarke, Edmund M., James R. Faeder, Christopher J. Langmead, Leonard A. Harris, Sumit Kumar Jha, and Axel Legay. "Statistical Model Checking in BioLab: Applications to the Automated Analysis of T-Cell Receptor Signaling Pathway." In Computational Methods in Systems Biology, 231–50. Berlin, Heidelberg: Springer Berlin Heidelberg, 2008. http://dx.doi.org/10.1007/978-3-540-88562-7_18.

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Ji, Ye, and Robert J. Woods. "Quantifying Weak Glycan-Protein Interactions Using a Biolayer Interferometry Competition Assay: Applications to ECL Lectin and X-31 Influenza Hemagglutinin." In Glycobiophysics, 259–73. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-2158-0_13.

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Bronswijk-Deddens, Lisette. "Epitope Binning of Human Monoclonal Antibodies in Classical Sandwich and In-Tandem Orientation Using the Octet System Based on Biolayer Interferometry." In Epitope Mapping Protocols, 207–20. New York, NY: Springer New York, 2018. http://dx.doi.org/10.1007/978-1-4939-7841-0_13.

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Pouliakis, Abraham, Aris Spathis, Christine Kottaridi, Antonia Mourtzikou, Marilena Stamouli, Stavros Archondakis, Efrossyni Karakitsou, and Petros Karakitsos. "Cloud Computing for BioLabs." In Cloud Technology, 1272–93. IGI Global, 2015. http://dx.doi.org/10.4018/978-1-4666-6539-2.ch058.

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Cloud computing has quickly emerged as an exciting new paradigm providing models of computing and services. Via cloud computing technology, bioinformatics tools can be made available as services to anyone, anywhere, and via any device. Large bio-datasets, highly complex algorithms, computing power demanding analysis methods, and the sudden need for hardware and computational resources provide an ideal environment for large-scale bio-data analysis for cloud computing. Cloud computing is already applied in the fields of biology and biochemistry, via numerous paradigms providing novel ideas stimulating future research. The concept of BioCloud has rapidly emerged with applications related to genomics, drug design, biology tools on the cloud, bio-databases, cloud bio-computing, and numerous applications related to biology and biochemistry. In this chapter, the authors present research results related to biology-related laboratories (BioLabs) as well as potential applications for the everyday clinical routine.
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Pouliakis, Abraham, Aris Spathis, Christine Kottaridi, Antonia Mourtzikou, Marilena Stamouli, Stavros Archondakis, Efrossyni Karakitsou, and Petros Karakitsos. "Cloud Computing for BioLabs." In Cloud Computing Applications for Quality Health Care Delivery, 228–49. IGI Global, 2014. http://dx.doi.org/10.4018/978-1-4666-6118-9.ch012.

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Cloud computing has quickly emerged as an exciting new paradigm providing models of computing and services. Via cloud computing technology, bioinformatics tools can be made available as services to anyone, anywhere, and via any device. Large bio-datasets, highly complex algorithms, computing power demanding analysis methods, and the sudden need for hardware and computational resources provide an ideal environment for large-scale bio-data analysis for cloud computing. Cloud computing is already applied in the fields of biology and biochemistry, via numerous paradigms providing novel ideas stimulating future research. The concept of BioCloud has rapidly emerged with applications related to genomics, drug design, biology tools on the cloud, bio-databases, cloud bio-computing, and numerous applications related to biology and biochemistry. In this chapter, the authors present research results related to biology-related laboratories (BioLabs) as well as potential applications for the everyday clinical routine.
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Conference papers on the topic "Biolabel"

1

Zhou, Wen, Joseph Um, Yali Zhang, Alexander Nelson, Bethanie Stadler, and Rhonda Franklin. "Ferromagnetic Resonance Characterization of Magnetic Nanowires for Biolabel Applications." In 2018 IEEE International Microwave Biomedical Conference (IMBioC). IEEE, 2018. http://dx.doi.org/10.1109/imbioc.2018.8428931.

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"Colorimetric Detection of Salmonella Typhimurium Using Enzyme Immobilization on Carbon Nanotubes as Biolabel." In International Conference on Chemical, Biological, and Environmental Sciences. International Academy Of Arts, Science & Technology, 2014. http://dx.doi.org/10.17758/iaast.a0514029.

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Novikova, A. S., and I. Yu Goryacheva. "Cd-free quantum dots for application as biolabels." In 2018 International Conference Laser Optics (ICLO). IEEE, 2018. http://dx.doi.org/10.1109/lo.2018.8435701.

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Gome, Gilad, Yuval Fein, Julian Waksberg, Yuval Maayan, Andrey Grishko, Iddo Yehoshua Wald, and Oren Zuckerman. "My First Biolab." In CHI '19: CHI Conference on Human Factors in Computing Systems. New York, NY, USA: ACM, 2019. http://dx.doi.org/10.1145/3290607.3313081.

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Fein, Yuval, Gilad Gome, Oren Zuckerman, and Hadas Erel. "My first biolab." In IDC '20: Interaction Design and Children. New York, NY, USA: ACM, 2020. http://dx.doi.org/10.1145/3397617.3402040.

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Moreira, Wendel L., Adriana Fontes, Andre Thomaz, Antonio A. Neves, Luiz C. Barbosa, Frederico D. de Menezes, Patricia M. A. de Farias, Beate S. Santos, and Carlos L. Cesar. "Synthesis and characterization of CdTe nanocrystals for applications as biolabels." In Biomedical Optics 2005, edited by Darryl J. Bornhop, Samuel I. Achilefu, Ramesh Raghavachari, and Alexander P. Savitsky. SPIE, 2005. http://dx.doi.org/10.1117/12.590841.

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Hornemann, Andrea, Diane Eichert, Sabine Flemig, Arne Hoehl, Gerhard Ulm, and Burkhard Beckhoff. "Probing biolabels for high throughput biosensing via synchrotron radiation SEIRA technique." In PROCEEDINGS OF THE 12TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION – SRI2015. Author(s), 2016. http://dx.doi.org/10.1063/1.4952927.

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Santos, Beate S., Patrícia M. A. de Farias, Frederico D. de Menezes, Erick L. Mariano, Ricardo de C. Ferreira, Selma Giorgio, Maira C. Bosetto, et al. "Molecular differentiation of leishmania protozoarium using CdS quantum dots as biolabels." In Biomedical Optics 2006, edited by Samuel Achilefu, Darryl J. Bornhop, and Ramesh Raghavachari. SPIE, 2006. http://dx.doi.org/10.1117/12.646912.

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Cotirlan, C., Marina Mustata, Sorin Miclos, Dan Savastru, Esofina Ristici, Doina Dimulescu, and M. Garais. "Nd:YAG laser for a Biolaser-1 ophthalmic system." In SPIE Proceedings, edited by Valentin I. Vlad. SPIE, 2004. http://dx.doi.org/10.1117/12.598042.

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Gong, Chaoyang, Yuan Gong, Maung Kyaw Khaing Oo, Yu Wu, Yunjiang Rao, and Xudong Fan. "Enzyme catalyzed optofluidic biolaser for sensitive ion concentration detection." In SPIE BioPhotonics Australasia, edited by Mark R. Hutchinson and Ewa M. Goldys. SPIE, 2016. http://dx.doi.org/10.1117/12.2244668.

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Reports on the topic "Biolabel"

1

Tallant, David Robert, Michael C. Wilson, Erik W. Leve, Hongyou Fan, C. Jeffrey Brinker, John Gabaldon, and Chessa Scullin. New self-assembled nanocrystal micelles for biolabels and biosensors. Office of Scientific and Technical Information (OSTI), December 2005. http://dx.doi.org/10.2172/877147.

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