Academic literature on the topic 'Biological assay/methods'

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Journal articles on the topic "Biological assay/methods"

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BARNARD, DONALD R. "BIOLOGICAL ASSAY METHODS FOR MOSQUITO REPELLENTS." Journal of the American Mosquito Control Association 21, sp1 (2005): 12–16. http://dx.doi.org/10.2987/8756-971x(2005)21[12:bamfmr]2.0.co;2.

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Davie, Sarah J., Kerry L. Whiting, and Barry J. Gould. "Biological Variation in Glycated Proteins." Annals of Clinical Biochemistry: International Journal of Laboratory Medicine 30, no. 3 (1993): 260–64. http://dx.doi.org/10.1177/000456329303000306.

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The high degree of individuality in the fructosamine assay has been ascribed to non-specific interferences in the assay. To investigate this, we measured the biological variability of 10 non-diabetic subjects using the fructosamine assay, the new fructosamine plus assay, glycated albumin and glycated total plasma proteins by affinity chromatography. The total variation of the two fructosamine assays was half that of the affinity chromatography assays. This was mainly due to the greater analytical imprecision of the affinity chromatography assays. The resulting high index of heterogeneity for b
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Krogh, Marie. "The Assay of Digitalis Substances by Biological Methods." Acta Medica Scandinavica 69, S26 (2009): 512–15. http://dx.doi.org/10.1111/j.0954-6820.1928.tb03938.x.

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Ubuka, Toshihiko. "Assay methods and biological roles of labile sulfur in animal tissues." Journal of Chromatography B 781, no. 1-2 (2002): 227–49. http://dx.doi.org/10.1016/s1570-0232(02)00623-2.

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SU, YI-CHENG, and AMY C. LEE WONG. "Current Perspectives on Detection of Staphylococcal Enterotoxins." Journal of Food Protection 60, no. 2 (1997): 195–202. http://dx.doi.org/10.4315/0362-028x-60.2.195.

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Staphylococcal food poisoning is one of the most economically important food-borne diseases in the United States, costing approximately $1.5 billion each year in medical expenses and loss of productivity. The amount of staphylococcal enterotoxin required to cause illness in humans depends on the susceptibility of the individuals. As little as 0.5 to 0.75 ng/ml of enterotoxin A in chocolate milk was shown to be able to cause illness in school children. Many methods have been developed for the detection of enterotoxins: immunological and biological assays. Immunological assays are more sensitive
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Yin, Zhimin. "Methods to Study the PVY Population in the Potato." Plant Breeding and Seed Science 75, no. 1 (2017): 71–76. http://dx.doi.org/10.1515/plass-2017-0010.

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Abstract The PVY population in the potato has been studied continuously using tobacco bait plants in potato fields at Młochów since 1980 at two-year intervals and in potato tuber samples collected from different regions of Poland since 2001 yearly. The paper presents the combined biological, serological and molecular assays for PVY identification and strain classification. Biologically, PVY strains are defined with respect to their ability to elicit hypersensitive resistance (HR) mediated by N genes in differential potato cultivars (King Edward, Desiree and Pentland Ivory) and to symptoms in t
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Oike, Takahiro, Yuka Hirota, Narisa Dewi Maulany Darwis, Atsushi Shibata, and Tatsuya Ohno. "Comparison of Clonogenic Survival Data Obtained by Pre- and Post-Irradiation Methods." Journal of Personalized Medicine 10, no. 4 (2020): 171. http://dx.doi.org/10.3390/jpm10040171.

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Clonogenic assays are the gold standard to measure in vitro radiosensitivity, which use two cell plating methods, before or after irradiation (IR). However, the effect of the plating method on the experimental outcome remains unelucidated. By using common cancer cell lines, here we demonstrate that pre-IR and post-IR plating methods have a negligible effect on the clonogenic assay-derived photon sensitivity as assessed by SF2, SF4, SF6, SF8, D10, or D50 (N.B. SFx indicates the survival at X Gy; Dx indicates the dose providing X% survival). These data provide important biological insight that s
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Ciapetti, G., P. Roda, L. Landi, A. Facchini, and A. Pizzoferrato. "In Vitro Methods to Evaluate Metal-Cell Interactions." International Journal of Artificial Organs 15, no. 1 (1992): 62–66. http://dx.doi.org/10.1177/039139889201500111.

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The aim of this study was to test different metals, widely employed in constructing prosthetic devices, by in vitro methods. Biological effects of such materials were analysed through four different assays on human lymphocytes and granulocytes. The lymphocyte proliferation assay gave quantitative results, while the viability test showed the morphological appearance of the cells correlated well with previous results. NK cytotoxicity and granulocyte chemokinesis tests provided interesting data on leucocyte performance when challenged with metals. Therefore the present study adds new basic inform
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Yu, Binbing, and Harry Yang. "Evaluation of Different Estimation Methods for Accuracy and Precision in Biological Assay Validation." PDA Journal of Pharmaceutical Science and Technology 71, no. 4 (2017): 297–305. http://dx.doi.org/10.5731/pdajpst.2016.007088.

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Kümmel, Anne, Hanspeter Gubler, Patricia Gehin, Martin Beibel, Daniela Gabriel, and Christian N. Parker. "Integration of Multiple Readouts into the Z' Factor for Assay Quality Assessment." Journal of Biomolecular Screening 15, no. 1 (2009): 95–101. http://dx.doi.org/10.1177/1087057109351311.

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Methods that monitor the quality of a biological assay (i.e., its ability to discriminate between positive and negative controls) are essential for the development of robust assays. In screening, the most commonly used parameter for monitoring assay quality is the Z' factor, which is based on 1 selected readout. However, biological assays are able to monitor multiple readouts. For example, novel multiparametric screening technologies such as high-content screening provide information-rich data sets with multiple readouts on a compound’s effect. Still, assay quality is commonly assessed by the
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Dissertations / Theses on the topic "Biological assay/methods"

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Shah, Ronak. "Autoxidation and its Inhibition in Both Industrial and Biological Contexts: New Molecules, Methods & Mechanisms." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/39838.

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Autoxidation, a radical chain reaction, is largely responsible for the degradation of most man-made and biological materials. These include chemical products such as lubricants, plastics and rubber; as well as biological molecules and membranes within our bodies. The development of means to hinder this process has been a major focus of the petroleum, chemical, pharmaceutical and biotechnology industry over the past century. The two most common strategies to emerge from these efforts have been the use of compounds that either prevent the initiation of autoxidation or trap the propagating radica
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Glezer, Andrea. ""Estudo da atividade biológica da macroprolactina humana em células Nb2 e em células Ba/F-03 transfectadas com o receptor de prolactina humano forma longa"." Universidade de São Paulo, 2006. http://www.teses.usp.br/teses/disponiveis/5/5135/tde-12042006-085305/.

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A macroprolactinemia é condição freqüente na hiperprolactinemia e em geral, sem impacto clínico. Os dados sobre a atividade biológica da macroprolactina (bbPRL) são controversos e baseados em bioensaio heterólogo com células de rato Nb2. A atividade biológica da bbPRL é observada in vitro e não in vivo, provavelmente porque seu alto peso molecular evita sua passagem pelos capilares. A bioatividade da bbPRL talvez varie de acordo com a especificidade do receptor de prolactina (PRLR). Avaliamos a bioatividade da bbPRL de indivíduos macroprolactinêmicos (Grupo I, n = 18) e da PRL monomérica (mPRL
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Santos, Iara Terezinha Queiroz Pereira dos. ""Avaliação da atividade clastogênica do resíduo catalítico industrial, por meio do bioensaio de micronúcleos com Tradescantia pallida cv. Purpurea"." Universidade de São Paulo, 2004. http://www.teses.usp.br/teses/disponiveis/5/5160/tde-04082005-131203/.

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O objetivo deste estudo foi aumentar o banco de dados em relação a resíduos (cake) e efluentes (licor) industriais e o seu nível de clastogenicidade. Este estudo contribuiu para mostrar: a) que o bioensaio com Tradescantia pallida foi sensível para a avaliação da clastogenicidade em mistura complexa de resíduos catalíticos industriais, nunca testados anteriormente. b) a tendência de uma dose resposta para ambos os resíduos catalíticos c)a pasta (cake) apresenta maior clastogenicidade que o licor nas concentrações estudadas. Provavelmente isto se deve a menor concentração de Ti e Al no licor do
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CHILAKALA, SUJATHA. "DEVELOPMENT OF LIQUID CHROMATOGRAPHY-MASS SPECTROMETRIC ASSAYS AND SAMPLE PREPARATION METHODS FOR THE BIOLOGICAL SAMPLE ANALYSIS." Cleveland State University / OhioLINK, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=csu1512927043412916.

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Arap, Marco Antonio. "Estudo da proteína de choque térmico GRP78 para o desenvolvimento de um sistema de receptor-ligante para o câncer de próstata." Universidade de São Paulo, 2003. http://www.teses.usp.br/teses/disponiveis/5/5153/tde-31052007-122749/.

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Introdução: Apesar dos avanços nas técnicas de diagnóstico e tratamento, o câncer de próstata avançado ainda é uma condição letal. Terapêuticas mais eficazes são necessárias para reduzir as taxas de morbi-mortalidade associadas à doença. A Proteína-78 regulada pela glicose (GRP78), uma proteína de choque térmico envolvida na apresentação de antígenos, foi recentemente descrita como sendo um possível marcador molecular para o câncer de próstata. Ainda mais, a resposta imune a essa proteína mostrou correlação com o desenvolvimento de doença hormônio-independente e com pior sobrevida para a doenç
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Kamoi, T. "Developing an optimal method for producing a tearless onion." Diss., Lincoln University, 2008. http://hdl.handle.net/10182/1008.

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People experience the irritating tearing and burning sensation of lachrymatory factor (LF, propanthial S-oxide) when cutting or chopping onion bulbs. LF is produced by lachrymatory factor synthase (LFS) specifically from 1-propenyl sulfenic acid, a breakdown product of trans-1-propenyl-L-cysteine sulfoxide (1-PRENCSO) by alliinase. This thesis describes strategies to produce a tearless onion by using RNA interference (RNAi) silencing. To determine whether a gene silencing cassette can silence lfs gene transcripts from onion (Allium cepa L.), a crop recalcitrant to genetic transformation, a gen
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Signorini, Allibe Nathalie. "Mesure de produits de photo-oxydation des bases de l'ADN isolé et cellulaire." Grenoble 1, 1998. http://www.theses.fr/1998GRE10056.

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Les radiations ionisantes, la composante ultraviolette du rayonnement solaire, ou des agents chimiques, peuvent provoquer des dommages de l'adn, comme des cassures de chaine, des modifications des sucres et des bases nucleiques. Compte-tenu du role capital que joue ce biopolymere dans la cellule, l'identification et la mesure de ces dommages sont fondamentaux pour la comprehension des phenomenes de degradation causes par ces differents facteurs. Une partie de ce manuscrit est consacree a l'utilisation de methodes immunologiques pour la detection et le dosage de defauts de l'adn. L'obtention d'
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Christ, Ana Paula. "DESENVOLVIMENTO E VALIDAÇÃO DE MÉTODOS ANALÍTICOS PARA O DOSEAMENTO DE DAPTOMICINA INJETÁVEL." Universidade Federal de Santa Maria, 2013. http://repositorio.ufsm.br/handle/1/5988.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior<br>This work presents the development and validation of analytical methods to assay daptomycin injection. Daptomycin is a drug of a new class of antibiotics, known as cyclic lipopeptides. It was approved in USA in 2003 and in Brazil in 2009. Up to now, there are no reports about methods to assay the drug in pharmaceutical products, both in scientific literature or in official compendia. Methods to identify daptomycin in raw material were realized as solubility, melting point, infrared spectrophotometry (IR), ultraviolet spectrophotome
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Montillet, Jean-Luc. "Dosage radioimmunologique du zinniol : application a l'etude de cette toxine dans l'alternariose de la carotte." Toulouse 3, 1986. http://www.theses.fr/1986TOU30208.

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Un dosage radioimmunologique specifique du zinniol a ete au point; les etapes de ce travail ont ete les suivantes: - synthese d'une molecule immunogene (conjugue zinniol-proteine porteuse). - obtention d'anticorps specifique (4 ci/mmole). - mise au point du dosage radioimmunologique valide du point de vue de sa specificite, de sa reproductibilite et de sa sensibilite (limite de detection 0,14 nn/essai). Cet outil a permis de realiser des dosages sur des plantes infectees. Ces resultats inedits montrent que la molecule est emise tres rapidement dans les tissus infectes (12 h apres inoculation).
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"Novel development of cell-based bioassays for biomedical applications." 2012. http://library.cuhk.edu.hk/record=b5549158.

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細胞為本生物檢測法(CBB)是指任何一種以細胞系統及其身上發生的生物反應為基礎原之直接檢測方法。在這篇文中,有關CBB 的工作大致分為個主要章節,描述如下:<br>第一章節探討以可調控式納米孔為基礎的電阻脈衝感應檢測法(RPS)進人血紅細胞的毒學研究。我們成功檢測到血紅細胞在低滲環境下的體型大小變化,並以式細胞儀及激光共焦掃描顯微鏡驗證上述實驗結果。此外,我們亦使用重皂甙(PD)及四溴雙酚A(TBBPA)種化合物以進毒試驗。在此章節,我們展示以RPS 在人體細胞系統的首次應用。這種RPS 無疑成為一種創新及低成本的技術,有助快速研究與血液有關的疾病。<br>第二章節探討以細胞為本的納米毒學研究。由於納米子已被廣泛應用於生物醫學上,它的應用同時帶出有關其毒性及其帶出的健康問題。在這章節,我們主要研究種同塗層的納米條(Au-NRs),其塗層分別為十烷基三甲基溴化氨(CTAB)及聚乙二醇(PEG)。透過一在人嗜鹼性細胞KU812 的毒實驗,我們展示Au-NRs 的生物相容性取決於它的塗層。CTAB 塗層的Au-NRs 會引發KU812 細胞死亡及其過敏性反應,而PEG 塗層的Au-NRs 則會引發以上反應。<br>Cell-based Bioassay (CBB) refers to any kind of assay which detection principle is based
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Books on the topic "Biological assay/methods"

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Cytokine bioassays: Methods and protocols. Humana Press, 2014.

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Statistical techniques in bioassay. Karger, 1988.

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Hubert, J. J. Bioassay. 3rd ed. Kendall/Hunt Pub. Co., 1992.

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Corey, Michael J. Coupled bioluminescent assays: Methods, evaluations, and applications. Wiley, 2009.

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Fujioka, Jeffrey T. Log-likelihood ratio tests for comparing dose-response data to the logistic function. National Oceanic and Atmospheric Administration, National Marine Fisheries Service, Northwest and Alaska Fisheries Center, Auke Bay Laboratory, 1986.

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Fujioka, Jeffrey T. Log-likelihood ratio tests for comparing dose-response data to the logistic function. National Oceanic and Atmospheric Administration, National Marine Fisheries Service, Northwest and Alaska Fisheries Center, Auke Bay Laboratory, 1986.

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Hormone assays in biological fluids. Humana Press, 2013.

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Vasodilator substances of the tissues. Cambridge University Press, 1986.

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Cell migration: Developmental methods and protocols. 2nd ed. Humana Press, 2011.

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William, Hewitt. Microbiological assay for pharmaceutical analysis: A rational approach. Interpharm/CRC, 2004.

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Book chapters on the topic "Biological assay/methods"

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Sen, Pranab K. "Biological Assay, Overview." In Methods and Applications of Statistics in Clinical Trials. John Wiley & Sons, Inc., 2014. http://dx.doi.org/10.1002/9781118596005.ch10.

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Lee, Scott J., Thomas A. Warnick, Susan B. Leschine, and Samuel P. Hazen. "A High-Throughput Biological Conversion Assay for Determining Lignocellulosic Quality." In Methods in Molecular Biology. Humana Press, 2012. http://dx.doi.org/10.1007/978-1-61779-995-2_18.

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Testa, Antonella, Valentina Palma, and Clarice Patrono. "Dicentric Chromosome Assay (DCA) and Cytokinesis-Block Micronucleus (CBMN) Assay in the Field of Biological Dosimetry." In Methods in Molecular Biology. Springer New York, 2019. http://dx.doi.org/10.1007/978-1-4939-9646-9_5.

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Gessner, Peter K., and Teresa Gessner. "Assay methods for disulfiram and metabolites in biological materials." In Disulfiram and its Metabolite, Diethyldithiocarbamate. Springer Netherlands, 1992. http://dx.doi.org/10.1007/978-94-011-2328-0_4.

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Khullar, Poonam, Lavanya Tandon, Rajpreet Kaur, and Divya Mandial. "Hemolytic Assay of Biocompatible Nanomaterials in Drug Delivery Systems." In Research Methods and Applications in Chemical and Biological Engineering. Apple Academic Press, 2019. http://dx.doi.org/10.1201/9780429424137-8.

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Conrad, Catharina, Miles A. Miller, Jörg W. Bartsch, Uwe Schlomann, and Douglas A. Lauffenburger. "Simultaneous Detection of Metalloprotease Activities in Complex Biological Samples Using the PrAMA (Proteolytic Activity Matrix Assay) Method." In Methods in Molecular Biology. Springer New York, 2017. http://dx.doi.org/10.1007/978-1-4939-6850-3_18.

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Grailer, Jamison, Richard A. Moravec, Zhijie Jey Cheng, et al. "Considerations in Developing Reporter Gene Bioassays for Biologics." In Methods in Pharmacology and Toxicology. Springer US, 2020. http://dx.doi.org/10.1007/978-1-0716-0171-6_9.

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Abstract The establishment of a robust and reproducible functional bioassay that reliably measures drug potency while ascertaining its mode of action is essential in biologic drug development. Here we describe a simple bioluminescent reporter gene bioassay for assessing biologics targeting immune checkpoints without the complexity and variability of more traditional assay systems. This chapter provides an overview of key considerations in reporter gene bioassay design and optimization, as well as development of thaw-and-use cells as an assay reagent for biologic QC lot release.
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Morovat, Alireza. "Methods for the Investigation of Thyroid Function." In Hormone Assays in Biological Fluids. Humana Press, 2013. http://dx.doi.org/10.1007/978-1-62703-616-0_5.

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Squire, Christine R. "Methods for the Investigation of Thyroid Function." In Hormone Assays in Biological Fluids. Humana Press, 2006. http://dx.doi.org/10.1385/1-59259-986-9:91.

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Sahagian, Khoren, and Mikki Larner. "Gas Plasma Surface Chemistry for Biological Assays." In Methods in Molecular Biology. Springer New York, 2015. http://dx.doi.org/10.1007/978-1-4939-2742-5_19.

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Conference papers on the topic "Biological assay/methods"

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Dutta, Debashis, and Naoki Yanagisawa. "Microfluidic Devices for Enhancing the Sensitivity of ELISA Methods." In ASME 2011 9th International Conference on Nanochannels, Microchannels, and Minichannels. ASMEDC, 2011. http://dx.doi.org/10.1115/icnmm2011-58284.

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Enzyme-linked immunosorbent assays (ELISA) are critically important tools in biological research, allowing the presence and concentrations of a wide variety of key biochemical intermediates to be determined. While the signal amplification that is the core advantage of ELISA methods is impressive, it is nevertheless the case that it is insufficient for some particularly demanding challenges in terms of sensitivity, assay time, or sample size. In this paper, we discuss three different approaches developed in our laboratory that can improve the sensitivity of ELISA methods by 2–3 orders of magnit
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Picard, P., J. Y. Borg, M. Vasse, et al. "BIOLOGICAL PRETHROMBOTIC MARKERS AND COAGULATION INHIBITORS IN NEPHROTIC SYNDROME." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643051.

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Nephrotic syndrome (NS) has long been recognized as a clinical prethrombotic state, because of severe thrombo-embolic complications. But underlying mechanisms are still poorly defined. In 56 untreated nephrotic adult patients (most of them without renal failure), we investigate in vivo coagulation activation by measuring 0 plasmatic specific fibrin degradation products (FbDP) (immuno-enzymological assay using a monoclonal anti D-neo antibody) and (2) the ratio of factor VII coagulant activity (V11c) to factor VII:Anti gen (VII;Ag) tested by an ELISA method. We examined coagulation inhibitors i
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Electricwala, A. "A SPECTROPHOTOMETRIC METHOD FOR FIBRIN CLOT LYSIS ASSAY." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1644839.

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Clot lysis assay is one of the many methods being used to quantitate tissue plasminogen activator in biological fluids and other media. It has been suggested that this assay may be less reproducible and subject to error due to variation between laboratories in determining the end point of clot lysis, especially at lower concentrations of the activator. It has been found that this assay method can be semi-automated by monitoring the absorbance of the fibrin clot, and determining the end point, in a spectrophotometer. Standard clots containing varying concentrations of tPA are prepared in semi-m
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Yum, Seungshic, Bong Gil Hyun, Kitae Rhie, and Kyoungsoon Shin. "ATP assay for rapid onboard testing to detect living microorganisms in Ballast Water." In IMarEST Ballast Water Technology Conference. IMarEST, 2017. http://dx.doi.org/10.24868/bwtc6.2017.012.

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Rapid and simple analytical methods for viable microorganism detection in ballast water are required to evaluate the efficiency of ballast water treatment system. During the course of systematic investigation of the cytotoxicity and apoptosis assays, it was found that the adenosine triphosphate (ATP) and luminescence based cell viability assay, in other word, an ATP assay was the most sensitive and applicable to ballast water management (BWM). The assay was applied to cultured microalgae samples, and it could detect the existence of 5 viable cells in 100 μl. Comparably low luminescent values w
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Madadi, Hojjat, and Jasmina Casals-Terré. "Study the Effects of Different Surfactants on Hydrophilicity of Polydimethylsiloxane (PDMS)." In ASME 2012 11th Biennial Conference on Engineering Systems Design and Analysis. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/esda2012-82399.

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The outstanding characteristics of polydimethylsiloxane (PDMS) caused its extensive use as base material to manufacture microfluidic devices. PDMS has numerous advantages coming from instinct properties such as its low cost, simple fabrication procedure, and robust nature that make it a compatible material in many applications such as biological and biomedical engineering. In spite of favorable physical and chemical properties, hydrophobic surface of PDMS is sometimes debatable. Because of PDMS is highly hydrophobic, pumping aqueous solution through microchannels using only capillary forces mi
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Ringwelski, Beth, Vidura Jayasooriya, and Dharmakeerthi Nawarathna. "Label Free Cell Purification Following Electroporation." In 2020 Design of Medical Devices Conference. American Society of Mechanical Engineers, 2020. http://dx.doi.org/10.1115/dmd2020-9037.

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Abstract Cell transfection by electroporation is a biological assay that has been utilized to inject exogenous molecules (e.g.: RNA, DNA and protein) into live cells. Recently, electroporation has been utilized in developing cell therapy for cancer (e.g., CAR T-cell). One of the major drawbacks in current electroporation methods is the cell death during the process. These dead cells can be detrimental, if injected back to the patients. Current cell filtering methods are unable purify T-cells following electroporation, this is due to the lack of unique biomarkers that target the apoptosis and n
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Boneu, B., G. Houin, M. Rostin, J. L. Montastructure, P. d’Azemar, and B. Bayrou. "INTER-INDIVIDUAL PHARMACOKINETIC VARIATIONS AFTER INTRAVENOUS (IV) AND SUBCUTANEOUS (SC) INJECTION OF CY 216 IN HEALTHY SUBJECTS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643235.

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We investigated the pharmacokinetic parameters and their inter individual variations of a low molecular weight heparin (LMWH) derivative (CY 216, Fraxiparine R, Choay). In a cross-over study, 100 anti Xa IC u/kg were injected in 12 healthy volunteers, either by IV or SC route, at one week interval. The pharmacological effects were followed on 12 serial citrated samples for 24h: - anti factor Xa (AXa) activity (chromogenic assay calibrated against CY 216); - APTT and thrombin clotting time prolongation. The main pharmacokinetic parameters (elimination half-life (T|); clearance (cl); distributio
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Dickerson, Samuel J., Steven P. Levitan, and Donald M. Chiarulli. "Nondestructive optical assay method for nanoscale biological particles in solution." In 2011 IEEE Winter Topicals (WTM). IEEE, 2011. http://dx.doi.org/10.1109/photwtm.2011.5730049.

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Casu, B., L. Marchese, A. Naggi, et al. "INFLUENCE OF THE SULFATION PATTERN ON CERTAIN BIOLOGICAL PROPERTIES OF GALACTOSAMINOGLYCANS." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643251.

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In order to investigate the influence of charge distribution and chain length on the biological properties of sulfated polysaccharides, additional sulfate groups were introduced into the galactosaminoglycans, chondriotin sulfate and dermatan sulfate. Using a flexible method (with sulfuric acid and chlorosulfonic acid) for concurrent sulfation and controlled depolymerization, numerous products were obtained and characterized by chemical, enzymatic and nuclear magnetic resonance spectroscopic methods. The biologic actions of these products were profiled in both in vitro and in vivo assays for an
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DELAHOUSSE, B., Y. GRUEL, P. MOALIC, L. QUILLIET, F. TOULEMONDE, and J. LEROY. "MODIFICATIONS OF BIOLOGICAL PARAMETERS DURING TREATMENT OF PULMONARY EMBOLISM BY A VERY LOW MOLECULAR HEPARIN FRAGMENT (CY 222)." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643230.

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45 patients with pulmonary embolism (PE) were treated by a very low molecular weight heparin fragment (CY 222, Institut CHOAY - France) in an open range dose study. Patients were included into three groups (I, II and III) and received respectively 500, 750 or 1000 IC (Institut Choay) antiXa units/ kg/day by continuous intravenous infusion for ten days. The laboratory screen carried out at Day 0 and at 2 - 8 - 12 - 24 - 36, 48 hours and then every day until Day 10, included : Platelet count, Thromboelastography (TEG) on platelet rich plasma (PRP), Amidolytic assays for anti Xa (CBS 3139 STAGO)
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Reports on the topic "Biological assay/methods"

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Lin, Emil T., Leslie Z. Benet, Robert A. Upton, and Winnie L. Gee. Analysis of Investigational Drugs in Biological Fluids - Method Development and Routine Assay. Defense Technical Information Center, 1991. http://dx.doi.org/10.21236/ada238981.

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