Academic literature on the topic 'Biomolecules Separation/Purification'

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Journal articles on the topic "Biomolecules Separation/Purification"

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Shil, Sumit, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi. "Biomolecular Liquid–Liquid Phase Separation for Biotechnology." BioTech 12, no. 2 (2023): 26. http://dx.doi.org/10.3390/biotech12020026.

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The liquid–liquid phase separation (LLPS) of biomolecules induces condensed assemblies called liquid droplets or membrane-less organelles. In contrast to organelles with lipid membrane barriers, the liquid droplets induced by LLPS do not have distinct barriers (lipid bilayer). Biomolecular LLPS in cells has attracted considerable attention in broad research fields from cellular biology to soft matter physics. The physical and chemical properties of LLPS exert a variety of functions in living cells: activating and deactivating biomolecules involving enzymes; controlling the localization, conden
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Gjulten, NEDJIP, and KARAOĞUL Eyyüp. "ADSORPTIVE BUBBLE SEPARATION METHODS (ABSM): FOAM FRACTION." International Journal of Current Naturalscience and Advance Phytochemistry, IJCNAP 1, no. 1/4 (2021): 1–17. https://doi.org/10.5281/zenodo.5810871.

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Foam fractionation is an ecological and economical method belonging to the adsorptive bubble separation techniques.  It represents an alternative to traditional methods used for the enrichment and isolation of biomolecules. Separation occurs due to the chemical or physical adsorption of surfactant molecules on the surface of the sprayed air bubble or the carrier gas rising through the liquid sample. This unit operation has the potential to use it as a low-cost industrial method for the enrichment and isolation of pharmaceutical biomolecules, proteins, enzymes, polymers, and secondary meta
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Corrêa, Priscila S., Wilson G. Morais Júnior, António A. Martins, Nídia S. Caetano, and Teresa M. Mata. "Microalgae Biomolecules: Extraction, Separation and Purification Methods." Processes 9, no. 1 (2020): 10. http://dx.doi.org/10.3390/pr9010010.

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Several microalgae species have been exploited due to their great biotechnological potential for the production of a range of biomolecules that can be applied in a large variety of industrial sectors. However, the major challenge of biotechnological processes is to make them economically viable, through the production of commercially valuable compounds. Most of these compounds are accumulated inside the cells, requiring efficient technologies for their extraction, recovery and purification. Recent improvements approaching physicochemical treatments (e.g., supercritical fluid extraction, ultras
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Mansurov, Z. A., A. R. Kerimkulova, S. A. Ibragimova, and E. Y. Gukenheimer. "Carbon Nanosorbent for Purification Different Biomolecules." Eurasian Chemico-Technological Journal 14, no. 1 (2011): 41. http://dx.doi.org/10.18321/ectj98.

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The article presents the results of physico-chemical studies on the development of nanostructured carbon materials from domestic raw materials. Were obtained and tested micro-mesoporous carbon sorbents for molecular-sieve chromatography of markers and investigated the applicability of carbon sorbents for the separation of protein-lipid complex, and plant bio-stimulator. Carbon sorbents have well-developed porous structure but their disadvantage is the weak mechanical<br />strength. Recently it was shown that some carbon nanostructures have enormous strength. Thus arose the need to give t
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Manali C. Asare, Jitendra A. Kubde, Ravindra L. Bakal, Pooja R. Hatwar, Vaishnavi S. Kalamb, and Pratik K. Tambakhe. "Ion exchange chromatography: A comprehensive review." GSC Biological and Pharmaceutical Sciences 31, no. 1 (2025): 026–37. https://doi.org/10.30574/gscbps.2025.31.1.0127.

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Chromatography is a crucial technique in various industries, particularly in pharmaceuticals, where it is used to separate, purify, and analyze compounds. Ion exchange chromatography (IEC) is a type of chromatography that separates ions based on their electrostatic interactions with a stationary phase. The technique is widely used in biotechnology, pharmaceutical processing, and water treatment. IEC is essential for the purification of charged proteins, nucleic acids, and other biomolecules. The separation process involves the interaction between ions and a charged stationary phase, allowing f
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Roberts, Michael W. H., Clarence M. Ongkudon, Gareth M. Forde, and Michael K. Danquah. "Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules." Journal of Separation Science 32, no. 15-16 (2009): 2485–94. http://dx.doi.org/10.1002/jssc.200900309.

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Labisch, Jennifer J., G. Philip Wiese, and Karl Pflanz. "Steric Exclusion Chromatography for Purification of Biomolecules—A Review." Separations 10, no. 3 (2023): 183. http://dx.doi.org/10.3390/separations10030183.

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Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without direct binding, such as covalent, electrostatic, and hydrophilic/hydrophobic interactions. The gentle nature of the method has led to an increased focus on sensitive targets such as enveloped viruses with potential for other sensitive entities, e.g., extracellular vesicles and virus-like particles. SX
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Ren, Yan, Yueping Bai, Zhidan Zhang, Wenlong Cai, and Antonio Del Rio Flores. "The Preparation and Structure Analysis Methods of Natural Polysaccharides of Plants and Fungi: A Review of Recent Development." Molecules 24, no. 17 (2019): 3122. http://dx.doi.org/10.3390/molecules24173122.

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Polysaccharides are ubiquitous biomolecules found in nature that contain various biological and pharmacological activities that are employed in functional foods and therapeutic agents. Natural polysaccharides are obtained mainly by extraction and purification, which may serve as reliable procedures to enhance the quality and the yield of polysaccharide products. Moreover, structural analysis of polysaccharides proves to be promising and crucial for elucidating structure–activity relationships. Therefore, this report summarizes the recent developments and applications in extraction, separation,
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Khoo, Kuan Shiong, Hui Yi Leong, Kit Wayne Chew, et al. "Liquid Biphasic System: A Recent Bioseparation Technology." Processes 8, no. 2 (2020): 149. http://dx.doi.org/10.3390/pr8020149.

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A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed.
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Najafi, Mesbah, and Margaret W. Frey. "Electrospun Nanofibers for Chemical Separation." Nanomaterials 10, no. 5 (2020): 982. http://dx.doi.org/10.3390/nano10050982.

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The separation and purification of specific chemicals from a mixture have become necessities for many environments, including agriculture, food science, and pharmaceutical and biomedical industries. Electrospun nanofiber membranes are promising materials for the separation of various species such as particles, biomolecules, dyes, and metals from liquids because of the combined properties of a large specific surface, light weight, high porosity, good connectivity, and tunable wettability. This paper reviews the recent progress in the design and fabrication of electrospun nanofibers for chemical
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Dissertations / Theses on the topic "Biomolecules Separation/Purification"

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Chibério, Abimaelle Silva. "Single-Column Chromatography with Recycle Lag Analog to Simulated Moving Bed Processes." Doctoral thesis, 2019. http://hdl.handle.net/10362/90890.

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The chromatography steps dominate the Downstream Processing (DSP) costs of a new biopharmaceutical, which has propelled the biopharmaceutical industry to invest a lot of effort on their improvement in order to reduce the costs of the biopharmaceutical production. Liquid chromatography (LC) is currently the core technique within the DSP strategy for the purification of biopharmaceuticals. Although single-column batch chromatography is simpler to operate than continuous (multicolumn) chromatography, the latter has many advantages over the former, including improved purity, yield, and prod
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Books on the topic "Biomolecules Separation/Purification"

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1949-, Asenjo Juan A., Hong Juan, and American Chemical Society Meeting, eds. Separation, recovery, and purification in biotechnology: Recent advances and mathematical modeling. American Chemical Society, 1986.

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1937-, Verrall M. S., and Hudson M. J. 1940-, eds. Separations for biotechnology. Published for the Society of Chemical Industry, London, by Ellis Harwood, 1987.

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K, Wang William, ed. Membrane separations in biotechnology. 2nd ed. M. Dekker, 2001.

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Affinity and Immunoaffinity Purification Techniques. EATON PUBLISHING, 2000.

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Separation and purification techniques in biotechnology. Noyes Publications, 1989.

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Desai, Mohamed A. Downstream Processing of Proteins: Methods and Protocols. Humana Press, 2010.

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Wang, William K. Membrane Separations in Biotechnology. Taylor & Francis Group, 2001.

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Wang, William K. Membrane Separations in Biotechnology, Second Edition, (Biotechnology and Bioprocessing Series). 2nd ed. CRC, 2001.

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Wang, William K. Membrane Separations in Biotechnology. Taylor & Francis Group, 2001.

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Wang, William K. Membrane Separations in Biotechnology. Taylor & Francis Group, 2019.

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Book chapters on the topic "Biomolecules Separation/Purification"

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Tjerneld, Folke, Patricia A. Alred, Richard F. Modlin, Antoni Kozlowski, and J. Milton Harris. "Purification of Biomolecules Using Temperature-Induced Phase Separation." In Aqueous Biphasic Separations. Springer US, 1995. http://dx.doi.org/10.1007/978-1-4615-1953-9_10.

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Hayes, Finbarr, and Daniela Barillà. "Overproduction, Separation and Purification of Affinity-Tagged Proteins from Escherichia coli." In Biomolecular and Bioanalytical Techniques. John Wiley & Sons, Ltd, 2019. http://dx.doi.org/10.1002/9781119483977.ch6.

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Prajapati, Vipul D. "Mesoporous Materials for Separation of Biomolecules." In Materials Research Foundations. Materials Research Forum LLC, 2025. https://doi.org/10.21741/9781644903452-14.

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Mesoporous materials have gained significant attention as advanced tools for the separation and purification of biomolecules due to their distinctive structural features, including higher surface areas, tunable pore sizes, and customizable surface functionalities. This chapter explores the synthesis, modification, and application of mesoporous materials in the separation of biomolecules such as proteins, enzymes, and nucleic acids. It provides a detailed overview of how pore size, surface chemistry, and material morphology influence the selectivity and efficiency of separation processes. The d
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Saha, S. "Applications of Ion Exchange Resins in Vitamins Separation and Purification." In Ion Exchange Resins. Materials Research Forum LLC, 2023. http://dx.doi.org/10.21741/9781644902219-2.

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Vitamins are vital compounds for the improved development and growth of the human body system. Together chemically and analytically, vitamins are divergent assembly of composites as they comprise of biomolecules. The source of vitamins is the necessary dietary food stuff. Small amounts of vitamins are needed to sustain a healthy life. Vitamins are generally classified into fat-soluble and water-soluble vitamins. The major portions of vitamins are vitamin A, vitamin B, vitamin C, vitamin E and vitamin K. This chapter discussed diverse procedures for the partition and purgation of vitamins B, C
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Vairaganthan, Pradheena, Gokilapriyavarthini Karthikeyan, and Darshini Subramanian. "BIO SEPARATION-A REMARKABLE PROGRESS FROM PREMATURE TO ADVANCED TECHNIQUE, AN UNPRECEDENTED ADVANCEMENT IN THE FIELD OF BIOTECHNOLOGY." In Futuristic Trends in Biotechnology Volume 3 Book 18. Iterative International Publishers, Selfypage Developers Pvt Ltd, 2024. http://dx.doi.org/10.58532/v3bkbt18p2ch2.

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Bioseparation is the large-scale purification of biological products utilising fundamental engineering and biological ideas. This abstract presents an overview of the remarkable expands and exceptional advancement in bioseparation strategies, which play a crucial role in medicine, biotechnology, and in food industry. The bioseparation uses has spread across numerous sectors, notably in biotechnology. Bioseparation applications were formerly simple, time-consuming, and poor yielding. However, tremendous progress has been made in recent years via the integration of analytical tools, computerizat
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Oke, Emmanuel A., and Sushma P. Ijardar. "Recapitulation on the separation and purification of biomolecules using ionic liquid-based aqueous biphasic systems." In Advanced Applications of Ionic Liquids. Elsevier, 2023. http://dx.doi.org/10.1016/b978-0-323-99921-2.00007-0.

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Kalyan, I., A. K. Nayak, and M. U. Khobragade. "Addressing the global water crisis: a comprehensive review of nanobiohybrid applications for water purification." In Nanobiohybrids for Advanced Wastewater Treatment and Energy Recovery. IWA Publishing, 2023. http://dx.doi.org/10.2166/9781789063592_0009.

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Abstract Systematic and organized handling of pre-existing and ever-emerging pollutants, which are continuously contaminating the freshwater resources and causing severe problems in aquatic bodies, aerial environments, and earthbound flora and fauna, is an absolute must. These pollutants contribute to one of the major global problems of scarcity of pristine water for daily use. In the last decades, different types of water cleaning and purification techniques have been embraced to address the issue. Traditional and standard water purification techniques have several shortcomings, like high tim
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Harrison, Roger G., Paul W. Todd, Scott R. Rudge, and Demetri P. Petrides. "Extraction." In Bioseparations Science and Engineering. Oxford University Press, 2015. http://dx.doi.org/10.1093/oso/9780195391817.003.0009.

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Extraction is a process in which two phases come into contact with the objective of transferring a solute or particle from one phase to the other. For the separation and purification of biological products, the phases are most commonly immiscible liquids, and the solute is in soluble form. In certain instances, however, one phase is a liquid and the other phase is a solid; the extraction of caffeine from coffee beans is one example. Although most extractions in biotechnology involve the transfer of soluble bioproducts, organelles and cells have at times been transferred between phases. An orga
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Singhal, Ramp, and Chenna R. Chakka. "Affinity separations of nucleic acids." In Affinity Separations. Oxford University PressOxford, 1997. http://dx.doi.org/10.1093/oso/9780199635504.003.0004.

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Abstract Recent advances in affinity chromatography have created a wide variety of important applications for the purification of diverse biomolecules, such as nucleic acids and their components, and antibodies, antigens, and enzymes. The affinity ligand is generally immobilized on an insoluble support and a mixture of compounds containing the molecule of interest is passed over the affinity support, usually in a chromatographic column. Ideally, the molecule of interest, having complementarity to the affinity support, binds to it while other molecules, exhibiting little or no binding affinity,
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Baines, Dev. "Analysis of purity." In Protein Purification Techniques. Oxford University Press, 2001. http://dx.doi.org/10.1093/oso/9780199636747.003.0007.

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In approaches to protein purification, the concepts of yield and purity are routinely used, but are often difficult to define in absolute terms. To some extent the purity of a protein sample will be defined by the final designated use of the product. In most cases, analyses will involve measurement of the mass of the protein in the sample and quantitation of specific property of the target molecule (e.g. activity) to provide values for the yield. Thus, the calculation of specific activity of given fractions through the purification process provides a valuable indication of the level of the pur
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