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Journal articles on the topic 'Biomolecules Separation/Purification'

Author: Grafiati

Published: 10 September 2021

Last updated: 29 July 2025

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1

Shil, Sumit, Mitsuki Tsuruta, Keiko Kawauchi, and Daisuke Miyoshi. "Biomolecular Liquid–Liquid Phase Separation for Biotechnology." BioTech 12, no. 2 (2023): 26. http://dx.doi.org/10.3390/biotech12020026.

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The liquid–liquid phase separation (LLPS) of biomolecules induces condensed assemblies called liquid droplets or membrane-less organelles. In contrast to organelles with lipid membrane barriers, the liquid droplets induced by LLPS do not have distinct barriers (lipid bilayer). Biomolecular LLPS in cells has attracted considerable attention in broad research fields from cellular biology to soft matter physics. The physical and chemical properties of LLPS exert a variety of functions in living cells: activating and deactivating biomolecules involving enzymes; controlling the localization, conden

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Gjulten, NEDJIP, and KARAOĞUL Eyyüp. "ADSORPTIVE BUBBLE SEPARATION METHODS (ABSM): FOAM FRACTION." International Journal of Current Naturalscience and Advance Phytochemistry, IJCNAP 1, no. 1/4 (2021): 1–17. https://doi.org/10.5281/zenodo.5810871.

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Foam fractionation is an ecological and economical method belonging to the adsorptive bubble separation techniques.  It represents an alternative to traditional methods used for the enrichment and isolation of biomolecules. Separation occurs due to the chemical or physical adsorption of surfactant molecules on the surface of the sprayed air bubble or the carrier gas rising through the liquid sample. This unit operation has the potential to use it as a low-cost industrial method for the enrichment and isolation of pharmaceutical biomolecules, proteins, enzymes, polymers, and secondary meta

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Corrêa, Priscila S., Wilson G. Morais Júnior, António A. Martins, Nídia S. Caetano, and Teresa M. Mata. "Microalgae Biomolecules: Extraction, Separation and Purification Methods." Processes 9, no. 1 (2020): 10. http://dx.doi.org/10.3390/pr9010010.

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Several microalgae species have been exploited due to their great biotechnological potential for the production of a range of biomolecules that can be applied in a large variety of industrial sectors. However, the major challenge of biotechnological processes is to make them economically viable, through the production of commercially valuable compounds. Most of these compounds are accumulated inside the cells, requiring efficient technologies for their extraction, recovery and purification. Recent improvements approaching physicochemical treatments (e.g., supercritical fluid extraction, ultras

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Mansurov, Z. A., A. R. Kerimkulova, S. A. Ibragimova, and E. Y. Gukenheimer. "Carbon Nanosorbent for Purification Different Biomolecules." Eurasian Chemico-Technological Journal 14, no. 1 (2011): 41. http://dx.doi.org/10.18321/ectj98.

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The article presents the results of physico-chemical studies on the development of nanostructured carbon materials from domestic raw materials. Were obtained and tested micro-mesoporous carbon sorbents for molecular-sieve chromatography of markers and investigated the applicability of carbon sorbents for the separation of protein-lipid complex, and plant bio-stimulator. Carbon sorbents have well-developed porous structure but their disadvantage is the weak mechanical<br />strength. Recently it was shown that some carbon nanostructures have enormous strength. Thus arose the need to give t

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Manali C. Asare, Jitendra A. Kubde, Ravindra L. Bakal, Pooja R. Hatwar, Vaishnavi S. Kalamb, and Pratik K. Tambakhe. "Ion exchange chromatography: A comprehensive review." GSC Biological and Pharmaceutical Sciences 31, no. 1 (2025): 026–37. https://doi.org/10.30574/gscbps.2025.31.1.0127.

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Chromatography is a crucial technique in various industries, particularly in pharmaceuticals, where it is used to separate, purify, and analyze compounds. Ion exchange chromatography (IEC) is a type of chromatography that separates ions based on their electrostatic interactions with a stationary phase. The technique is widely used in biotechnology, pharmaceutical processing, and water treatment. IEC is essential for the purification of charged proteins, nucleic acids, and other biomolecules. The separation process involves the interaction between ions and a charged stationary phase, allowing f

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Roberts, Michael W. H., Clarence M. Ongkudon, Gareth M. Forde, and Michael K. Danquah. "Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules." Journal of Separation Science 32, no. 15-16 (2009): 2485–94. http://dx.doi.org/10.1002/jssc.200900309.

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Labisch, Jennifer J., G. Philip Wiese, and Karl Pflanz. "Steric Exclusion Chromatography for Purification of Biomolecules—A Review." Separations 10, no. 3 (2023): 183. http://dx.doi.org/10.3390/separations10030183.

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Steric exclusion chromatography (SXC) is a purification method that is based on steric exclusion effects from the surface of the target and a hydrophilic stationary phase after the addition of polyethylene glycol (PEG), which leads to an association of the target with the stationary phase without direct binding, such as covalent, electrostatic, and hydrophilic/hydrophobic interactions. The gentle nature of the method has led to an increased focus on sensitive targets such as enveloped viruses with potential for other sensitive entities, e.g., extracellular vesicles and virus-like particles. SX

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Ren, Yan, Yueping Bai, Zhidan Zhang, Wenlong Cai, and Antonio Del Rio Flores. "The Preparation and Structure Analysis Methods of Natural Polysaccharides of Plants and Fungi: A Review of Recent Development." Molecules 24, no. 17 (2019): 3122. http://dx.doi.org/10.3390/molecules24173122.

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Polysaccharides are ubiquitous biomolecules found in nature that contain various biological and pharmacological activities that are employed in functional foods and therapeutic agents. Natural polysaccharides are obtained mainly by extraction and purification, which may serve as reliable procedures to enhance the quality and the yield of polysaccharide products. Moreover, structural analysis of polysaccharides proves to be promising and crucial for elucidating structure–activity relationships. Therefore, this report summarizes the recent developments and applications in extraction, separation,

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Khoo, Kuan Shiong, Hui Yi Leong, Kit Wayne Chew, et al. "Liquid Biphasic System: A Recent Bioseparation Technology." Processes 8, no. 2 (2020): 149. http://dx.doi.org/10.3390/pr8020149.

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A well-known bioseparation technique namely liquid biphasic system (LBS) has attracted many researchers’ interest for being an alternative bioseparation technology for various kinds of biomolecules. The present review begins with an in-depth discussion on the fundamental principle of LBS and this is followed by the discussion on further development of various phase-forming components in LBS. Additionally, the implementation of various advance technologies to the LBS that is beneficial towards the efficiency of LBS for the extraction, separation, and purification of biomolecules was discussed.

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Najafi, Mesbah, and Margaret W. Frey. "Electrospun Nanofibers for Chemical Separation." Nanomaterials 10, no. 5 (2020): 982. http://dx.doi.org/10.3390/nano10050982.

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The separation and purification of specific chemicals from a mixture have become necessities for many environments, including agriculture, food science, and pharmaceutical and biomedical industries. Electrospun nanofiber membranes are promising materials for the separation of various species such as particles, biomolecules, dyes, and metals from liquids because of the combined properties of a large specific surface, light weight, high porosity, good connectivity, and tunable wettability. This paper reviews the recent progress in the design and fabrication of electrospun nanofibers for chemical

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Wijethunga, Anushi Madushani, and Chijioke Emenike. "Separation methods for food protein purification and analysis." Exploration of Foods and Foodomics 2, no. 4 (2024): 391–407. http://dx.doi.org/10.37349/eff.2024.00043.

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The extraction, separation, and purification of dietary proteins from a variety of food sources are crucial for their targeted use in food applications. To achieve this, proteins should be effectively separated from non-protein components such as cell wall structures, polysaccharides, and lipids. Traditional protein purification methods can be time-consuming, highlighting the need for automated, cost-effective, and sustainable alternatives. This comprehensive review critically assesses various protein purification instruments from an analytical perspective, weighing their advantages and disadv

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Chen, Wei, Zihui Meng, Min Xue, and Kenneth J. Shea. "Molecular imprinted photonic crystal for sensing of biomolecules." Molecular Imprinting 4, no. 1 (2016): 1–12. http://dx.doi.org/10.1515/molim-2016-0001.

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AbstractMolecularly imprinted polymers (MIPs) are highly cross-linked polymers with high binding capacity and selectivity to the target molecules. MIPs become increasingly important because of the potential applications in drug delivery, purification and separation. In spite of the tremendous progress that has been made in the molecular imprinting field, many challenges remain to be addressed, especially in transforming the binding event into a detectable optical signal. The combination of photonic crystal and molecular imprinting technique is becoming a popular research idea. Compared to the

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ADACHI, Tadashi, Noriyuki YASUDA, and Shouhei OHARA. "Development of Ion Exchange Separation Media for Purification of Biomolecules at High Throughput." Journal of Ion Exchange 31, no. 2 (2020): 22–27. http://dx.doi.org/10.5182/jaie.31.22.

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Kim, Nayeong, Jiho Lee, Jemin Jeon, Johannes Elbert, and Xiao Su. "Electrochemical Recovery of Biomolecules in Redox-Mediated Electrodialysis Platform." ECS Meeting Abstracts MA2024-01, no. 55 (2024): 2911. http://dx.doi.org/10.1149/ma2024-01552911mtgabs.

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In the chemical and biochemical manufacturing industry, the recovery and purification of organic species are amongst the most energy-demanding steps, due to the complexity of product streams, involving unreacted reactants, byproducts, inorganic salts along with the final products.1, 2 Membrane-integrated electrochemical methods have garnered interest as promising candidates for selective recovery of organic molecules, such as whey protein from cheese whey waste.3 Nonetheless, they still encounter a bottleneck in discriminating among charged species, primarily due to the lack of intrinsic ion s

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Rocha, L. A., A. I. C. Gonçalves, D. Bicho, R. Martins, and F. Silva. "Screening of gellan gum as an ionic and hydrophobic chromatographic matrix for biomolecules purification." Separation and Purification Technology 132 (August 2014): 452–60. http://dx.doi.org/10.1016/j.seppur.2014.06.004.

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De Luca, Chiara, Giulio Lievore, Desiree Bozza, et al. "Downstream Processing of Therapeutic Peptides by Means of Preparative Liquid Chromatography." Molecules 26, no. 15 (2021): 4688. http://dx.doi.org/10.3390/molecules26154688.

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The market of biomolecules with therapeutic scopes, including peptides, is continuously expanding. The interest towards this class of pharmaceuticals is stimulated by the broad range of bioactivities that peptides can trigger in the human body. The main production methods to obtain peptides are enzymatic hydrolysis, microbial fermentation, recombinant approach and, especially, chemical synthesis. None of these methods, however, produce exclusively the target product. Other species represent impurities that, for safety and pharmaceutical quality reasons, must be removed. The remarkable producti

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Xu, Wenzhuo, Xinpei Gao, Liqiang Zheng, and Fei Lu. "Ionic-Liquid-Based Aqueous Two-Phase Systems Induced by Intra- and Intermolecular Hydrogen Bonds." Molecules 27, no. 16 (2022): 5307. http://dx.doi.org/10.3390/molecules27165307.

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In recent years, aqueous two-phase systems (ATPSs) have been widely used in different fields and have become an increasingly attractive subject due to their application in the separation and purification of biomolecules. In this work, the aqueous phase behavior of ionic liquids (ILs) was modulated by changing the cis-trans structure of the anion in ILs. With the same tetra-butyl-phosphine as the cation, the cis-anion exhibited upper critical solution temperature (UCST) phenomena. In contrast, the trans-anion exhibited lower critical solution temperature (LCST) phenomena. The proposed mechanism

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Kim, S. H., M. J. Kim, and Y. H. Choa. "Fabrication and estimation of Au-coated Fe3O4 nanocomposite powders for the separation and purification of biomolecules." Materials Science and Engineering: A 449-451 (March 2007): 386–88. http://dx.doi.org/10.1016/j.msea.2006.02.438.

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Lievore, Giulio, Desiree Bozza, Martina Catani, et al. "Benefits of a Mixed-Mode Stationary Phase to Address the Challenging Purification of an Industrially Relevant Peptide: A Proof-of-Concept Study." Separations 9, no. 5 (2022): 125. http://dx.doi.org/10.3390/separations9050125.

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Peptides are a class of biomolecules with a great potential from the therapeutic point of view, because of their unique biological properties. Industrially, the production stategies adopted produce both the target peptide and a series of impurities that must be removed. Preparative chromatography is the technique of choice for the large-scale purification of biomolecules, generally performed in reversed-phase mode, using hydrophobic adsorbents (e.g., C8 stationary phases). A promising and innovative alternative is represented by mixed-mode columns, which bear two different ligands on the parti

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Silva, Josiane F. da, Clara M. G. Lima, Débora L. da Silva, et al. "Lectin Purification through Affinity Chromatography Exploiting Macroporous Monolithic Adsorbents." Separations 10, no. 1 (2023): 36. http://dx.doi.org/10.3390/separations10010036.

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Growing medical, engineering, biochemical, and biological interest has led to a steady pace of research and development into polymeric monolithic structures with densely interconnected pores for purifying bio compounds. Cryogels, which are generated by freezing a reactive polymerization mixture, are highlighted due to their versatility and low relative cost as macroporous, polymeric, monolithic adsorbents. The conversion of cryogels into affinity adsorbents is one possible alternative to their optimal application. Some of the most often utilized supports for immobilizing particular ligands are

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Pereira, Jorge F. B., Sónia P. M. Ventura, Francisca A. e Silva, Shahla Shahriari, Mara G. Freire, and João A. P. Coutinho. "Aqueous biphasic systems composed of ionic liquids and polymers: A platform for the purification of biomolecules." Separation and Purification Technology 113 (July 2013): 83–89. http://dx.doi.org/10.1016/j.seppur.2013.04.004.

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22

Hassannejad, Sahar. "Liquid Biphasic System." Pharmacy and Applied Health Sciences 1, no. 1 (2022): 29–42. http://dx.doi.org/10.59480/phahs.v1i1.10.

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Many researchers have been interested in a substitute bioseparation technology for different biomolecules, namely the liquid biphasic system (L.B.S.). The analysis starts with a detailed discussion of the basic concept of L.B.S. and follows by a debate on the further creation of the different components for phase-forming of L.B.S. Also, various advance L.B.S. technologies will be introduced. It was addressed that is beneficial to L.B.S.'s e-function for bimolecular extraction, separation, and purification. The L.B.S. key parameters for link were obtainable and assessed. Furthermore, a guidelin

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Barut, Miloš, Aleš Podgornik, Peter Brne, and Aleš Štrancar. "Convective Interaction Media short monolithic columns: Enabling chromatographic supports for the separation and purification of large biomolecules." Journal of Separation Science 28, no. 15 (2005): 1876–92. http://dx.doi.org/10.1002/jssc.200500246.

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Martín-Banderas, Lucía, Román González-Prieto, Alfonso Rodríguez-Gil, et al. "Application of Flow Focusing to the Break-Up of a Magnetite Suspension Jet for the Production of Paramagnetic Microparticles." Journal of Nanomaterials 2011 (2011): 1–10. http://dx.doi.org/10.1155/2011/527437.

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Paramagnetic particles offer an extensive improvement in the magnetic separation or purification of a wide variety of protein molecules. Most commercial paramagnetic particles are synthesized by laborious and costly procedures. A straightforward production of paramagnetic microparticles with homogeneous and selectable sizes using flow focusing (FF) technology is described in this work. The development of an initial formulation of a stable iron oxide suspension compatible with the FF requirements is also reported. The obtained particles, below 10 microns in diameter and presenting smooth and re

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Hatami Giklou Jajan, Leila, Seyed Nezamedin Hosseini, Mohsen Abolhassani, and Masoud Ghorbani. "Progress in affinity ligand-functionalized bacterial magnetosome nanoparticles for bio-immunomagnetic separation of HBsAg protein." PLOS ONE 17, no. 7 (2022): e0267206. http://dx.doi.org/10.1371/journal.pone.0267206.

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Efficient Bio-immunomagnetic separation (BIMS) of recombinant hepatitis B surface antigen (rHBsAg) with high binding capacity was studied using affinity ligand immobilized bacterial magnetosome nanoparticles (Magnetospirillum gryphiswaldense strain MSR-1 bacteria) as an immunomagnetic sorbent. Our results showed immunomagnetic adsorption, acted by affinity interactions with the immobilized monoclonal antibody, offered higher antigen adsorption and desorption capacities as compared with the commercially available immunoaffinity sorbents. Four different ligand densities of the Hep-1 monoclonal a

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González Fernández, Cristina, Jenifer Gómez Pastora, Arantza Basauri, et al. "Continuous-Flow Separation of Magnetic Particles from Biofluids: How Does the Microdevice Geometry Determine the Separation Performance?" Sensors 20, no. 11 (2020): 3030. http://dx.doi.org/10.3390/s20113030.

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The use of functionalized magnetic particles for the detection or separation of multiple chemicals and biomolecules from biofluids continues to attract significant attention. After their incubation with the targeted substances, the beads can be magnetically recovered to perform analysis or diagnostic tests. Particle recovery with permanent magnets in continuous-flow microdevices has gathered great attention in the last decade due to the multiple advantages of microfluidics. As such, great efforts have been made to determine the magnetic and fluidic conditions for achieving complete particle ca

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Blanco, María, Ana C. Sánchez, Begoña Correa, et al. "Production and Partial Characterization of Bioactive Compounds from Underutilized Marine Bioresources for a Cosmetic Formulation: Cytotoxicity and Bioactivity Evaluation." International Journal of Molecular Sciences 24, no. 20 (2023): 15380. http://dx.doi.org/10.3390/ijms242015380.

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Hydrolyzed collagen, glycogen, and hyaluronic acid, obtained through the biotechnological valorization of underutilized marine bioresources, fulfill cosmetic industry requirements for sustainable products produced under circular economy principles. Hydrolyzed collagen was obtained by hydrolyzing blue shark collagen with papain and ultrafiltration. Glycogen was isolated from industrial mussel cooking wastewaters through ultrafiltration, precipitation, and selective polysaccharide separation. Hyaluronic acid was produced by fermentation, purification, and depolymerization. The main objective was

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Hussein, Abdulhakeem D., Manal Q. Mohammed, Hajer Hussein, and Hajer A. Mejbil. "Isolation and Purification of Amylase from Serum of Patients with Pancreatitis and Comparing the Biochemical Properties with Amylase Purified from Healthy People." Journal of Biotechnology Research Center 16, no. 2 (2022): 14–21. http://dx.doi.org/10.24126/jobrc.2022.16.2.639.

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Back Ground: Amylases are a group of enzymes that hydrolyze starch into simple sugars. Amylase is secreted in the human body from saliva and the pancreas. Abnormal levels of the enzyme amylase indicate pancreatitis. Enzyme purification eliminates various proteins and other forms of biomolecules while restoring the majority of enzyme activity. Objectives: Isolation and purification of the alpha-amylase enzyme from the serum of a patient with pancreatitis and a healthy human, and estimation of the values of Michalis constant Km as well as the maximum velocity Vmax to determine the affini

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Bakhshpour, Monireh, Neslihan Idil, Işık Perçin, and Adil Denizli. "Biomedical Applications of Polymeric Cryogels." Applied Sciences 9, no. 3 (2019): 553. http://dx.doi.org/10.3390/app9030553.

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The application of interconnected supermacroporous cryogels as support matrices for the purification, separation and immobilization of whole cells and different biological macromolecules has been well reported in literature. Cryogels have advantages over traditional gel carriers in the field of biochromatography and related biomedical applications. These matrices nearly mimic the three-dimensional structure of native tissue extracellular matrix. In addition, mechanical, osmotic and chemical stability of cryogels make them attractive polymeric materials for the construction of scaffolds in tiss

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Szucsova, Jaroslava, Adriana Zelenakova, Lubos Nagy, et al. "Influence of Ligands on Physicochemical Characteristics of Magnetic Nanoparticles." Journal of Nano Research 78 (April 17, 2023): 91–96. http://dx.doi.org/10.4028/p-2x4090.

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Magnetic-bead separation or purification serves as a technique for effective isolation of biomolecules. In presented work we prepared and characterized core-shell magnetic nanoparticle samples consisted of Fe3O4 core coated with SiO2 shell. Samples were subsequently coated with ligands MPTMS (3-(mercaptopropyl)trimethoxysilane), CPTMS (3-(chloropropyl)trimethoxysilane) and MMSP (3-(trimethoxysilyl)propyl methacrylate) with aim to increase the number of active centers for specific binding with RNA. Such samples were further investigated for their magnetic properties, size, and morphology. Magne

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Lemma, Solomon Mengistu, Cristiana Boi, and Ruben G. Carbonell. "Nonwoven Ion-Exchange Membranes with High Protein Binding Capacity for Bioseparations." Membranes 11, no. 3 (2021): 181. http://dx.doi.org/10.3390/membranes11030181.

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This study presents the preparation and characterization of UV-grafted polybutylene terepthalate (PBT) ion exchange nonwoven membranes for chromatographic purification of biomolecules. The PBT nonwoven was functionalized with sulfonate and secondary amine for cation and anion exchange (CEX and AEX), respectively. The anion exchange membrane showed an equilibrium static binding capacity of 1300 mg BSA/g of membrane, while the cationic membranes achieved a maximum equilibrium binding capacity of over 700 mg hIgG/g of membrane. The CEX and AEX membranes resulted in dynamic binding capacities unde

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Alves, Mariana B. N., André M. Lopes, Nathalia A. Santos, Valéria C. Santos-Ebinuma, Filipa A. Vicente, and Jorge F. B. Pereira. "Influence of Choline Chloride on the Phase Equilibria and Partition Performance of Polymer/Polymer Aqueous Biphasic Systems." Separations 10, no. 10 (2023): 528. http://dx.doi.org/10.3390/separations10100528.

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A series of polymeric aqueous biphasic systems (ABS) were determined using polyethylene glycol (PEG) and sodium polyacrylate (NaPA) with choline chloride ([Ch]Cl) as an adjuvant. The effect of (i) PEG and NaPA molecular weights, (ii) PEG functionalization, (iii) [Ch]Cl addition (at different concentrations), and (iv) temperature (25, 37 and 50 °C) was evaluated through their ability to promote the two-phase separation. The results showed that the polymerization degree and functionalization of PEG polymers exhibit a large influence on the ABS formation, with high molecular weight PEG inducing a

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Kim, S. H., Jae Kyo Yang, K. J. Lee та Yong Ho Choa. "Fabrication and Estimation of Au Coated γ-Fe₂O₃ Nanoparticles for Separation and Purification of Biomolecules". Key Engineering Materials 317-318 (серпень 2006): 235–38. http://dx.doi.org/10.4028/www.scientific.net/kem.317-318.235.

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Au coated γ-Fe2O3 nanoparticles were prepared by the changing of surface charge of Au and γ-Fe2O3 nanoparticles with pH. Well Au coated γ-Fe2O3 nanocomposite particles developed in the range between isoelectric point of Au and that of γ-Fe2O3 nanoparticles. Immobilization of glutathione (GSH), crystalline polypeptide containing mercapto group, was successfully performed on the well Au coated γ-Fe2O3 nanocomposite particles. Properties of nanoparticles and GSH immobilized Au/γ-Fe2O3 were characterized by UV-vis, LPA, XRD, and TEM.

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Kawano, Katsumi, Yuki Hashikura, and Kunihiko Umekita. "Purification Method of Extracellular Vesicles Derived from Human T-Cell Leukemia Virus Type 1-Infected Cells without Virions." Viruses 16, no. 2 (2024): 249. http://dx.doi.org/10.3390/v16020249.

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To mediate intercellular communication, cells produce extracellular vesicles (EVs). These EVs transport many biomolecules such as proteins, nucleic acids, and lipids between cells and regulate pathophysiological actions in the recipient cell. However, EVs and virus particles produced from virus-infected cells are of similar size and specific gravity; therefore, the separation and purification of these two particles is often controversial. When analyzing the physiological functions of EVs from virus-infected cells, the presence or absence of virus particle contamination must always be verified.

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Schwaminger, S. P., S. A. Blank-Shim, I. Scheifele, P. Fraga-García, and S. Berensmeier. "Peptide binding to metal oxide nanoparticles." Faraday Discussions 204 (2017): 233–50. http://dx.doi.org/10.1039/c7fd00105c.

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Magnetic metal oxide nanoparticles demonstrate great applicability in several fields such as biotechnology, medicine and catalysis. A stable, magnetic and low-cost material, nanoscale magnetite, is an interesting adsorbent for protein purification. Downstream processing can account for up to 80% of the total production costs in biotechnological production. As such, the development of new innovative separation methods can be regarded as highly profitable. While short peptide sequences can be used as specific affinity tags for functionalised adsorber surfaces, they need expensive affinity ligand

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Urzì, Ornella, Roberta Gasparro, Nima Rabienezhad Ganji, Riccardo Alessandro, and Stefania Raimondo. "Plant-RNA in Extracellular Vesicles: The Secret of Cross-Kingdom Communication." Membranes 12, no. 4 (2022): 352. http://dx.doi.org/10.3390/membranes12040352.

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The release of extracellular vesicles (EVs) is a common language, used by living organisms from different kingdoms as a means of communication between them. Extracellular vesicles are lipoproteic particles that contain many biomolecules, such as proteins, nucleic acids, and lipids. The primary role of EVs is to convey information to the recipient cells, affecting their function. Plant-derived extracellular vesicles (PDEVs) can be isolated from several plant species, and the study of their biological properties is becoming an essential starting point to study cross-kingdom communication, especi

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Katalevskiy, Artem D., Kirill V. Smirnov, and Natalia N. Smirnova. "MEMBRANES IN BIOTECHNOLOGY: CURRENT STATE AND PROSPECTS." ChemChemTech 68, no. 1 (2024): 6–22. https://doi.org/10.6060/ivkkt.20256801.7075.

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Membranes have always been an important part of a diverse range of biotechnological processes. Nowadays, reverse osmosis, ultrafiltration, microfiltration, gas separation, chromatography, pervaporation, electrodialysis and other membrane-based processes are integral part of biotechnological production, enabling the resolution of various technological challenges. First membrane systems, used in biotechnology was taken from other fields. However, over the past 45 years, new materials and components have been developed for specific biotechnological applications. Membranes are highly suitable for

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Muhammad, Fazal-ur-Rehman. "Methodological trends in preparation of activated carbon from local sources and their impacts on production: A review." Chemistry International 4, no. 2 (2018): 109–19. https://doi.org/10.5281/zenodo.1475350.

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Activated carbon, also known as activated charcoal, which is crude form of graphite, substance which is used lead pencils. Activated carbon is widely used in dye removal and also has other applications. Activated carbon has high surface area, adsorption capacity, and high adsorption rates from the gas or liquid phases. Activated carbon is also used in air purification, chromatography, energy storage, electrode materials for li-ion batteries biosensors, hydrogen storage, immobilizing the biomolecules. Therefore, activated carbon has wide applications. It is used in gas separation, solvents reco

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Eilts, Friederike, Marleen Steger, Keven Lothert, and Michael W. Wolff. "The Suitability of Latex Particles to Evaluate Critical Process Parameters in Steric Exclusion Chromatography." Membranes 12, no. 5 (2022): 488. http://dx.doi.org/10.3390/membranes12050488.

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The steric exclusion chromatography (SXC) is a rather new method for the purification of large biomolecules and biological nanoparticles based on the principles of precipitation. The mutual steric exclusion of a nonionic organic polymer, i.e., polyethylene glycol (PEG), induces target precipitation and leads to their retention on the chromatographic stationary phase. In this work, we investigated the application of latex particles in the SXC by altering the particle’s surface charge as well as the PEG concentration and correlated both with their aggregation behavior. The parameters of interest

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Oliveira, Filipa M., Teresa G. Nunes, Nadya V. Dencheva, and Zlatan Z. Denchev. "Structure and Molecular Dynamics in Metal-Containing Polyamide 6 Microparticles." Crystals 12, no. 11 (2022): 1579. http://dx.doi.org/10.3390/cryst12111579.

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Polymer microparticles are used in additive manufacturing, separation and purification devices, biocatalysis, or for the recognition of biomolecules. This study reports on the effect of metal fillers on the structure and molecular dynamics of polyamide 6 (PA6) microparticles (MPs) containing up to 19 wt.% of Al, Cu, or Mg. These hybrid MPs are synthesized via reactive microencapsulation by anionic ring-opening polymerization in solution, in the presence of the metal filler. 13C high-resolution solid-state NMR (ssNMR) spectroscopy is employed as the primary characterization method using magic a

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Ma, Tengfei, and Peng Liu. "Magnetic Nanomaterials in Chinese Medicine Chemical Composition Analysis and Drug Metabolism and Its Industry Prospect and Development Path Research." Journal of Chemistry 2020 (September 30, 2020): 1–8. http://dx.doi.org/10.1155/2020/1234269.

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The paramagnetism and superparamagnetism of magnetic nanomaterials are very important for in vivo applications. The magnetic particles with paramagnetism or superparamagnetism can redistribute the magnetic particles after the magnetic field is withdrawn, which is widely used for the separation and purification of biomolecules. At the same time, superparamagnetic particles can also be used as MRI imaging contrast agent. Compared with Western medicine, traditional Chinese medicine is different from Western medicine in that it is originated from nature and has thousands of years of clinical effic

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Mansurov, Z. A., J. M. Jandosov, A. R. Kerimkulova, et al. "Nanostructured Carbon Materials for Biomedical Use." Eurasian Chemico-Technological Journal 15, no. 3 (2013): 209. http://dx.doi.org/10.18321/ectj224.

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One of the priority trends of carbon nanotechnology is creation of nanocomposite systems. Such carbon nanostructured composites were produced using - raw materials based on the products of agricultural waste, such as grape stones, apricot stones, rice husk. These products have a - wide spectrum of application and can be obtained in large quantities. The Institute of Combustion Problems has carried out the work on synthesis of the nanostructured carbon sorbents for multiple applications including the field of biomedicine. The article presents the data on the synthesis and physico-chemical prope

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Koul, Saloni, Mamata Singhvi, and Beom Soo Kim. "Green Synthesis of Cobalt-Doped CeFe2O5 Nanocomposites Using Waste Gossypium arboreum L. Stalks and Their Application in the Removal of Toxic Water Pollutants." Nanomaterials 14, no. 16 (2024): 1339. http://dx.doi.org/10.3390/nano14161339.

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Currently, there is an increasing need to find new ways to purify water by eliminating bacterial biofilms, textile dyes, and toxic water pollutants. These contaminants pose significant risks to both human health and the environment. To address this issue, in this study, we have developed an eco-friendly approach that involves synthesizing a cobalt-doped cerium iron oxide (CCIO) nanocomposite (NC) using an aqueous extract of Gossypium arboreum L. stalks. The resulting nanoparticles can be used to effectively purify water and tackle the challenges associated with these harmful pollutants. Nanopa

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Price, C. A. "Partition of Cell Particles and Macromolecules. Separation and Purification of Biomolecules, Cell Organelles, Membranes, and Cells in Aqueous Polymer Two-Phase Systems and Their Use in Biochemical Analysis and Biotechnology.Per Ake Albertsson." Quarterly Review of Biology 62, no. 1 (1987): 74–75. http://dx.doi.org/10.1086/415300.

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Hübner, J., R. Heinzler, C. Arlt, S. Hohmann, G. Brenner-Weiß, and M. Franzreb. "An automated and compartmented fluidic reactor device for multi-step sample-to-answer processes using magnetic particles." Reaction Chemistry & Engineering 2, no. 3 (2017): 349–65. http://dx.doi.org/10.1039/c6re00219f.

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Kates, Patrick A., John J. Tomashek, David A. Miles, and L. Andrew Lee. "Parallel sample processing using dispersive INtip micro-purification on programmable multichannel pipettes." BioTechniques 68, no. 3 (2020): 148–54. http://dx.doi.org/10.2144/btn-2019-0140.

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Automation gives researchers the ability to process and screen orders of magnitude higher numbers of samples than manual experimentation. Current biomacromolecule separation methodologies suffer from necessary manual intervention, making their translation to high-throughput automation difficult. Herein, we present the first characterization of biomacromolecule affinity purification via dispersive solid-phase extraction in a pipette tip (INtip). We use commercially available resin and compare efficiency with batch and spin column methodologies. Moreover, we measure the kinetics of binding and e

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Mahajan, Kalpesh D., Gang Ruan, Greg Vieira, et al. "Biomolecular detection, tracking, and manipulation using a magnetic nanoparticle-quantum dot platform." Journal of Materials Chemistry B 8, no. 16 (2020): 3534–41. http://dx.doi.org/10.1039/c9tb02481f.

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Fluorescent and magnetic materials play a significant role in biosensor technology, enabling sensitive quantification and separations with applications in diagnostics, purification, quality control, and therapeutics.

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Peters, T. J. "Partition of cell particles and macromolecules: Separation and purification of biomolecules, cell organelles, membranes and cells in aqueous polymer two phase systems and their use in biochemical analysis and biotechnology. P-A. Albertsson. Third Edition, 1986, John Wiley and Sons, Chichester, £61.35 pages 346." Cell Biochemistry and Function 5, no. 3 (1987): 233–34. http://dx.doi.org/10.1002/cbf.290050311.

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Lu, Wei, Georges Grevillot, and Laurence Muhr. "ESIEX - electrical swing ion exchange: pH control for biomolecule purifications or separations." Desalination and Water Treatment 14, no. 1-3 (2010): 1–6. http://dx.doi.org/10.5004/dwt.2010.1005.

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Simanjuntak, Berlian, Helen Julian, and M. T. A. P. Kresnowati. "Downstream Process of Xylanase Production from Oil Palm Empty Fruit Bunches: A Review." IOP Conference Series: Earth and Environmental Science 1034, no. 1 (2022): 012046. http://dx.doi.org/10.1088/1755-1315/1034/1/012046.

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Abstract As an enzyme that can hydrolyze the glycosidic bond of hemicellulose, xylanase is widely used in pulp and paper, feed, food, and beverage industries. The vast availability of lignocellulosic biomass waste offers the potential of xylanase production by microorganisms via submerged and solid-state fermentation. However, the specific activity of the produced crude enzyme is still too low to be directly used in a commercial application. Downstream processing of the enzyme, that is purification and concentration, is necessary. Centrifugation or filtration can be used as a pre-treatment pro

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