Academic literature on the topic 'Biopesticides'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the lists of relevant articles, books, theses, conference reports, and other scholarly sources on the topic 'Biopesticides.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Journal articles on the topic "Biopesticides"
1
., Nidhi, Saba Mushtaq, Gladys Saji, and Vinod Kumar Gupta. "MODE OF ACTION OF BIOPESTICIDES AGAINST PESTS AND FUTURE PROSPECTS OF BIOPESTICIDES AND NANOBIOPESTICIDES." Journal of Advanced Scientific Research 13, no. 11 (December 31, 2022): 01–08. http://dx.doi.org/10.55218/jasr.2022131101.
Full textAbstract:
Biopesticides, and nano-biopesticides such as viruses, bacteria, fungi, algae, and nematodes are currently being used in a huge amount and their importance is increasing day by day because pesticides are already created a harmful effects on our health and environment. Due to the chemicals pesticides, diseases are increased in a high rate and food quality is decreased because toxicity level is increased. Biopesticides are best alternative to chemicals pesticides and best results to control pest control programs. The virulence of many biopesticides such as bacteria, fungus, and plant product were tested in laboratory and evaluated under field conditions and successfully approval by government. Biopesticide products are now sold in market for the control of pest and diseases. The aim of biopesticide research is to make these biopesticide products available at field level at a reasonable price and this would become a possible step in the integrated pest management strategy. Moreover, biopesticide research is still going on and in future more products are needed in aspects of bioformulation and areas such as commercialization. This review paper has the important and basic mode of action of major biopesticides in the past. The future prospects for the development of new biopesticides are also discussed such as nano- biopesticides.
2
Gupta, Ipsa, Rishikesh Singh, Suganthi Muthusamy, Mansi Sharma, Kamaljit Grewal, Harminder Pal Singh, and Daizy R. Batish. "Plant Essential Oils as Biopesticides: Applications, Mechanisms, Innovations, and Constraints." Plants 12, no. 16 (August 10, 2023): 2916. http://dx.doi.org/10.3390/plants12162916.
Full textAbstract:
The advent of the “Green Revolution” was a great success in significantly increasing crop productivity. However, it involved high ecological costs in terms of excessive use of synthetic agrochemicals, raising concerns about agricultural sustainability. Indiscriminate use of synthetic pesticides resulted in environmental degradation, the development of pest resistance, and possible dangers to a variety of nontarget species (including plants, animals, and humans). Thus, a sustainable approach necessitates the exploration of viable ecofriendly alternatives. Plant-based biopesticides are attracting considerable attention in this context due to their target specificity, ecofriendliness, biodegradability, and safety for humans and other life forms. Among all the relevant biopesticides, plant essential oils (PEOs) or their active components are being widely explored against weeds, pests, and microorganisms. This review aims to collate the information related to the expansion and advancement in research and technology on the applications of PEOs as biopesticides. An insight into the mechanism of action of PEO-based bioherbicides, bioinsecticides, and biofungicides is also provided. With the aid of bibliometric analysis, it was found that ~75% of the documents on PEOs having biopesticidal potential were published in the last five years, with an annual growth rate of 20.51% and a citation per document of 20.91. Research on the biopesticidal properties of PEOs is receiving adequate attention from European (Italy and Spain), Asian (China, India, Iran, and Saudi Arabia), and American (Argentina, Brazil, and the United States of America) nations. Despite the increasing biopesticidal applications of PEOs and their widespread acceptance by governments, they face many challenges due to their inherent nature (lipophilicity and high volatility), production costs, and manufacturing constraints. To overcome these limitations, the incorporation of emerging innovations like the nanoencapsulation of PEOs, bioinformatics, and RNA-Seq in biopesticide development has been proposed. With these novel technological interventions, PEO-based biopesticides have the potential to be used for sustainable pest management in the future.
3
II Ujah, JI Ugochukwu, and UB Alozieuwa. "An evaluation of phytochemical and biopesticidal composition of scent leaf." GSC Biological and Pharmaceutical Sciences 17, no. 1 (October 30, 2021): 117–23. http://dx.doi.org/10.30574/gscbps.2021.17.1.0281.
Full textAbstract:
Phytochemicals are therapeutic while biopesticides are naturally occurring forms pesticides which are eco-friendly. The phytochemicals as well biopesticidal contents of scent leaf were investigated. The result indicated that glycosides and alkaloids not detectable, phenols and terpenoids least present, flavonoids and saponins moderately present while steroids and tannins were high in abundance.The result as investigated showed that scent leaf extract contains various secondary metabolites in the following concentrations, saponins (0.130 ± 0.361 mg/g), tannins (0.133 ± 0.365 mg/g), flavonoid (0.033 ± 182 mg/g) steroid (0.005 ± 0.071 mg/g), terpenoid (0.071 ± 0.266 mg/g) as well as oxalic acid which is the biopesticidal content. Owing to the phytochemicals and biopesticide in this leaf, scent leaf could be used both therapeutically and as a biopesticide.
4
Rusdi and Amalia Rusaldy. "The Effectiveness of Fragrant Biopesticide of Lemon Cigarette Biopesticide to Control Fruit Flies on Large Chilli Plants." IOP Conference Series: Earth and Environmental Science 1153, no. 1 (May 1, 2023): 012030. http://dx.doi.org/10.1088/1755-1315/1153/1/012030.
Full textAbstract:
Abstract Problems that are often encountered in increasing the production of large chili plants are pests and diseases. Some important pests that generally attack chili plants are armyworms, aphids, fruit flies, and mites. The study aimed to obtain citronella biopesticides that were effective in controlling fruit fly pests on large chili plants, using a randomized block design (RBD) with 5 levels of treatment and 5 repetitions. Biopesticide treatment consisted of citronella ash biopesticide (Ts4), citronella extract (Ts3), citronella oil (Ts2), existing (Ts1), and without biopesticide (Ts0). The results showed that citronella oil biopesticides (Ts2) had better potency than other biopesticides and controls. All citronella biopesticide treatments have low effectiveness against fruit fly pests (<40%). The main weather factor is high-intensity rain which greatly affects the effectiveness of biopesticide in the process of controlling fruit flies. Economically, the application of citronella biopesticides technology and existing biopesticides is beneficial for businesses. The value of income per hectare from large chili farming starts from the highest to the lowest respectively: treatment Ts2 (income Rp. 60,977,000 with a B/C ratio = 2.640), Ts3 (income Rp. 57,852,000 with a B/C ratio = 2.516), Ts1 (income Rp. 57,527,000 with a B/C ratio = 2.504), Ts4 (income Rp. 53,952,000 with a B/C ratio = 2.346) and Ts0 (income Rp. 46,852,000 with a B/C ratio = 2.171). With an average B/C ratio of > 1, it means that the citronella biopesticide technology is feasible to be developed to control fruit fly pests on large chili plants.
5
Soyel, Sk Amir, Subhajit Ruidas, Pritam Roy, Swagata Mondal, Sudip Bhattacharyya, and Dipak Hazra. "Biopesticides as Eco-friendly Substitutes to Synthetic Pesticides: An Insight of Present Status and Future Prospects with Improved Bio-effectiveness, Self-lives, and Climate Resilience." International Journal of Environmental Sustainability and Protection 2, no. 2 (June 30, 2022): 1–12. http://dx.doi.org/10.35745/ijesp2022v02.02.0001.
Full textAbstract:
Synthetic pesticides are well-known not just for their efficiency in pest control, but also for their adverse effects on humans, ecology, and living biome's ecological relationships. Biopesticides are biodegradable, environment-friendly, and non-destructive insecticides that are safe for creatures. They leave no harmful residues in food or feed. Botanical, microbiological, and biochemical biopesticides are the three types of biopesticides. 3,000 tons of biopesticides are produced every year, accounting for around 2.5% of the overall pesticide market. Biopesticides are currently accessible in a variety of forms, including dust, granules, powders, emulsions, suspension concentrates, and so on. The Central Insecticide Board and Registration Committee (CIBRC) in India requires data on chemistry, bioefficacy, toxicity, packaging, and labeling to register biopesticides. Although India has registered 970 biopesticide products, lesser effectiveness, shorter shelf-life, lack of knowledge, and lengthy registration process remain hurdles. New and improved formulation methods might improve biopesticide efficacies, self-lives, and climate resiliency while lowering prices and making them economically competitive with synthetic chemicals. The main types of biopesticides, their benefits and downsides, worldwide and Indian market scenarios, formulations, registration procedures, and current advancements for users and environment-friendly applications and sustainable agriculture are all covered in this review article.
6
Ruiu, Luca. "Microbial Biopesticides in Agroecosystems." Agronomy 8, no. 11 (October 23, 2018): 235. http://dx.doi.org/10.3390/agronomy8110235.
Full textAbstract:
Microbial biopesticides include several microorganisms like bacteria, fungi, baculoviruses, and nematode-associated bacteria acting against invertebrate pests in agro-ecosystems. The biopesticide sector is experiencing a significant growth and many discoveries are being developed into new biopesticidal products that are fueling a growing global market offer. Following a few decades of successful use of the entomopathogenic bacterium Bacillus thuringiensis and a few other microbial species, recent academic and industrial efforts have led to the discovery of new microbial species and strains, and of their specific toxins and virulence factors. Many of these have, therefore, been developed into commercial products. Bacterial entomopathogens include several Bacillaceae, Serratia, Pseudomonas, Yersinia, Burkholderia, Chromobacterium, Streptomyces, and Saccharopolyspora species, while fungi comprise different strains of Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, Verticillium, Lecanicillium, Hirsutella, Paecilomyces, and Isaria species. Baculoviruses are species-specific and refer to niche products active against chewing insects, especially Lepidopteran caterpillars. Entomopathogenic nematodes (EPNs) mainly include species in the genera Heterorhabditis and Steinernema associated with mutualistic symbiotic bacteria belonging to the genera Photorhabdus and Xenorhabdus. An updated representation of the current knowledge on microbial biopesticides and of the availability of active substances that can be used in integrated pest management programs in agro-ecosystems is reported here.
7
Damascena, Arthaka, Divanissa Kusumaningrum, Emi Widiyanti, Ines Fitriana, Lintang Mukti, Perdana Mangayu Bagyo, Rival Fairuz, Salsyabila Fajrin, and Samuel Suryowidhi. "Training on making biopesticides as effort to strengthen organic agriculture by Taruna Tani Lestari." Abdimas: Jurnal Pengabdian Masyarakat Universitas Merdeka Malang 8, no. 4 (November 30, 2023): 559–71. http://dx.doi.org/10.26905/abdimas.v8i4.11307.
Full textAbstract:
Pests are one of the problems in agriculture. The use of organic pesticides still needs to be developed in Gentungan Village. Training in making biopesticides is useful for controlling pests and diseases using environmentally friendly natural ingredients. In addition, to foster the ability of Taruna Tani Lestari in Gentungan Village to make biopesticides independently and disseminate them to local farmers. The implementation method involved presentations, questions and answers, practices, modules, and evaluation designs. The material presented included the meaning and benefits of biopesticides, the natural ingredients used, the correct dosage, and the stages of making biopesticides. After the training, the output obtained included members of Taruna Tani Lestari knowing the natural ingredients for making biopesticides, members of Taruna Tani Lestari understanding the benefits of using biopesticides, members of Taruna Tani Lestari being able to make biopesticides independently and educate local farmers and display biopesticide products.
8
Xiao, Liping, Heping Zhu, Matthew Wallhead, Leona Horst, Peter Ling, and Charles R. Krause. "Characterization of Biological Pesticide Deliveries through Hydraulic Nozzles." Transactions of the ASABE 61, no. 3 (2018): 897–908. http://dx.doi.org/10.13031/trans.12698.
Full textAbstract:
Abstract. Increased use of biopesticides has spurred demand for efficient and effective delivery systems. To this goal, laboratory tests were conducted to investigate six different classes of biopesticides discharged from five different types of flat-fan nozzles, each with three flow capacities. The biopesticide classes were horticultural oil insecticide (HOI), bacterial biofungicide (BBF), botanical extract bio-insecticide (BEBI), liquid fungal bio-insecticide (FBI-ES), wettable powder fungal bio-insecticide (FBI-WP), and mineral salt biofungicide (MSBF). Droplet size distribution, spray pattern width, spray deposition, and coverage on plant leaves and water-sensitive paper (WSP) were the spray parameters tested. These parameters varied greatly with biopesticide class, nozzle type, and nozzle flow capacity. The DV0.5 of the biopesticides ranged from 75 to 519 µm for 0.76 L min-1 nozzles, from 86 to 509 µm for 1.51 L min-1 nozzles, and from 99 to 622 µm for 2.25 L min-1 nozzles. The use of different biopesticides with the same nozzle significantly altered the spray pattern width. Compared to a water-only solution, the largest change of spray pattern width was 14.7% for the air-induction nozzle (AI11004) discharging FBI-ES. The amount of spray deposited on plant leaves for all tested biopesticides (except HOI) did not vary significantly with different nozzle types. However, the air-induction nozzles produced the most uneven spray deposition patterns. Therefore, to achieve optimal spray application efficiency and effectiveness for discharging specific classes of biopesticide, the choice of the proper nozzle type and flow capacity is critical. Keywords: Biopesticide, Crop protection, Deposition, Droplet size, Spray coverage.
9
Irma Suryani, A., Itji Diana Daud, Melina, Vien Sartika Dewi, Dian Ekawati Sari, Sulfiani, Suriati Eka Putri, and Hasri. "Harnessing The Potential Of Nanobiopesticides With Plant Extracts: A Review." IOP Conference Series: Earth and Environmental Science 1272, no. 1 (December 1, 2023): 012042. http://dx.doi.org/10.1088/1755-1315/1272/1/012042.
Full textAbstract:
Abstract This article aims to provide an overview of nanobiopesticides as a promising solution for sustainable and environmentally friendly pest control in agriculture. These formulations combine nanotechnology and biopesticides derived from plant extracts. The synthesis and characterization of plant extract-based pesticide nanoparticles are found to be more effective than using biopesticides alone. In this review, first, we examine the literature on the use of biopesticides, including both plant extract-based pesticides and biopesticides from microorganisms. Moreover, we review several studies on the combination of plant extract-based pesticides and biopesticides to enhance biopesticide efficacy. Finally, we discuss nanobiopesticides in supporting sustainable and environmentally friendly agriculture by utilizing biopesticides based on nanoparticles. Overall, this review highlights the promising prospects of plant extract-based nanobiopesticides and underscores their potential as effective and sustainable alternatives for pest management in modern agriculture.
10
Haryuni, Haryuni, Andre Fahriz Perdana Harahap, Supartini, Achmadi Priyatmojo, and Misri Gozan. "The Effects of Biopesticide and Fusarium oxysporum f.sp. vanillae on the Nutrient Content of Binucleate Rhizoctonia-Induced Vanilla Plant." International Journal of Agronomy 2020 (April 28, 2020): 1–6. http://dx.doi.org/10.1155/2020/5092893.
Full textAbstract:
Binucleate Rhizoctonia (BNR) fungi are essential for the germination of vanilla seeds. Chemical control of the soil-borne pathogen might adversely affect BNR. The purpose of this study is to determine the effect of Nicotiana tabacum extract biopesticides and Fusarium oxysporum f.sp. vanillae (Fusarium) on vanilla plant nutrient content induced by BNR. Materials and Methods. The research design was completely randomized design with two factors and three replications. The first factor was biopesticide (dosage of 0, 10, 20, and 30 ml/seedling), and the second factor was the application of Fusarium. Results. The increase in the nitrogen, phosphorus, and potassium content of vanilla was affected by biopesticides and Fusarium inoculation. Fusarium inoculation has no significant effect on nitrogen and phosphorus levels but significantly affects potassium levels. The biopesticide dosage is significant for nitrogen, phosphorus, and potassium. The interaction of biopesticides with Fusarium inoculation did not significantly affect the parameters of nitrogen and phosphorus content, but significantly affected potassium content. Conclusion. The application of biopesticides and Fusarium inoculation after induction of BNR can increase nitrogen, phosphorus, and potassium content of vanilla plants.
Dissertations / Theses on the topic "Biopesticides"
1
Paoli, Pier Paolo. "Novel sources of biopesticides." Thesis, University of Newcastle Upon Tyne, 2011. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.555978.
Full textAbstract:
In recent years changing agricultural conditions are leading to insect pests becoming a more serious threat to UK crop production for a variety of local and global factors. Two important factors in these changes are: (i) increasing public intolerance of the use of exogenously applied chemical insecticides which can have negative effects both in terms of public health (through exposure to residues on food crops) and the environment; and (ii) changes in the distribution and abundance of insect pests as a result of increased mean temperatures resulting from global climate change, which will alter the pest breeding habits and periods of damage. In terms of environmental effects, are the effects on non-target organisms including beneficial insects that play a role in the suppression of insect pest populations. To control pests, the development of novel pesticides should focus on non-chemical alternatives derived from natural sources. In the present study venom proteins were isolated and purified from the parasitoid wasp Eulophus pennicornis (Hymenoptera: Eulophidae) and the sea anemone Actinia equina (Actiniaria: Actiniidae) by gel filtration on a 16/60 HR Sephacryl and anion exchange FPLC chromatography (Pharmacia-LKB Mono Q HR 5/5) in order to evaluate their potential toxicity towards lepidopteran (Lacanobia oleracea) and dipteran (Musca domestica) larvae. Characterisation of the parasitoid venom by DNA sequencing of a venom gland cDNA library demonstrated the presence of trypsin-like serine proteases (NM_001170877), insulin-like growth factors (NP _001082137), late trypsin (XP _001604401), and metalloproteases (XP _001599927.1). Venom proteins from the sea anemone were identified by mass spectrometry (MALDI TOF/TOF) as actinoporins (Q9Y1U9) and Na+ and K+ channel inhibitors which are known to act as neurotoxins (P61541, Q9NDA5, P81791). Genes encoding purified parasitoid proteins were subsequently expressed in Pichia pastoris as a microbial expression system and the late trypsin recombinant protein was tested for insecticidal activity. Sea anemone toxins, on the other hand, were purified from source for subsequent bioassay. The recombinant proteins and protein fractions were then tested for their insecticidal activity by injection into L. oleracea and M domestica larvae. Anti-moulting factor and juvenile hormone esterase inhibition factor purified from the parasitoid venom were shown to cause between 70-100% and 50-90% mortality respectively in L. oleracea after a period of 14 days, with observable physiological changes beginning on day 7; the recombinant late trypsin protein (XP _ 001604401) was shown to cause 70% mortality in L. oleracea larvae after 8 days. Out of 30 fractions isolated from sea anemone acrorhagi using gel.
2
Thiec, Marie Alliot Anne. "La lutte biologique et les biopesticides." [S.l.] : [s.n.], 2007. http://castore.univ-nantes.fr/castore/GetOAIRef?idDoc=22986.
Full text
3
Rodríguez, Serrano Paula. "Valorización de digestato como sustrato para la producción de biopesticidas con Bacillus thuringiensis mediante fermentación en estado sólido." Doctoral thesis, Universitat Autònoma de Barcelona, 2019. http://hdl.handle.net/10803/669776.
Full textAbstract:
Aquesta tesi és part del projecte DECISIVE, finançat pel programa europeu H2020 (acord de subvenció N° 689.229), que té com a objectiu proposar i provar un esquema de gestió descentralitzada per a la valorització dels residus sòlids orgànics municipals. L'objectiu general d'aquesta investigació és la producció de bioproductes de valor afegit a partir del digestat de fracció orgànica de residus sòlids municipals com a substrat mitjançant l'ús de la fermentació en estat sòlid (FES). El principal bioproducte a explorar és un biopesticida inoculant el digestat amb Bacillus thuringiensis (Bt), conegut per les seves propietats biopesticides.
En primer lloc, es van realitzar proves a escala laboratori per comprovar la idoneïtat del digestat per a l'obtenció de diferents bioproductes (cel·lulases, soforolípids, proteases i biopesticides), aconseguint els millors resultats de producció per al biopesticida produït per Bt. Aquests experiments es van realitzar amb digestat estèril i digestat higienitzat, observant que aquest microorganisme era capaç de créixer i esporular utilitzant digestat higienitzat. Posteriorment, es van estudiar un seguit d'estratègies d’operació en reactors aïllats tèrmicament de 10-L amb digestat higienitzat. El procés es va escalar a un reactor de 100-L de llit empacat sense aïllament i amb agitació, observant una esporulació del 89% amb un creixement d'espores 3 vegades més gran que l'inicial, mostrant produccions de biopesticida a partir de Bt.
Posteriorment, es va realitzar una comparació entre dues soques de Bt, Bacillus thuringiensis subespècie kurstaki (Btk) i Bacillus thuringiensis subespècie israelensis (Bti). Es va avaluar la diferència de les dues soques a escala laboratori 0.5-L observant els efectes d'esterilització, la higienització i la congelació del digestat en la producció de cèl·lules viables i espores per Btk i Bti. Paral·lelament, es van realitzar proves de conservació, anàlisi microbiològic i bioassaigs del digestat.
Amb tots els resultats anteriors, els últims anàlisis realitzats en aquesta tesi van ser l'optimització de l'extracció i caracterització de la toxina produïda per Bt a partir del cristall proteic, concentrat i conservat a través de tècniques com: la disrupció, on es van disminuir les cèl·lules viables i es va incrementar la producció d'espores, desprenent el cristall proteic i la liofilització per conservar el material, on es va observar un 86 i 89% de recuperació de les espores per Btk i Bti. Es va realitzar una purificació del substrat, determinant les proteïnes característiques de Bt amb efecte biopesticida a partir d'una electroforesi.
Aquesta tesi és un pas endavant per comprovar les característiques del cristall proteic de Bt amb efecte biopesticida a partir de FES i poder utilitzar el producte final valoritzant així el residu del tractament de la fracció orgànica de residus municipals.Esta tesis es parte del proyecto DECISIVE, financiado por el programa europeo H2020 (acuerdo de subvención N ° 689229), que tiene como objetivo proponer y probar un esquema de gestión descentralizada para la valorización de los residuos sólidos orgánicos urbanos. El objetivo general de esta investigación es la producción de bioproductos de valor añadido a partir del digestato de fracción orgánica de residuos sólidos municipales como sustrato mediante el uso de la Fermentación en Estado Sólido (FES). El principal producto a explorar es un biopesticida a obtener inoculando el residuo con Bacillus thuringiensis (Bt), que produce un cristal proteico con propiedades biopesticidas. En primer lugar, se realizaron pruebas a escala laboratorio para comprobar la idoneidad del digestato para la obtención de diferentes bioproductos (celulasas, soforolípidos, proteasas y biopesticidas), alcanzando los mejores resultados de producción para el biopesticida producido por Bt. Posteriormente, se probaron una serie de estrategias de producción en reactores aislados térmicamente de 10-L con digestato higienizado pasando a escalar el proceso a un reactor de 100-L de lecho empacado sin aislamiento y con agitación observando una esporulación del 89% con un crecimiento de esporas 3 veces mayor que el inicial. Posteriormente, se realizó una comparación entre dos cepas de Bt, Bacillus thuringiensis subespecie kurstaki (Btk) y Bacillus thuringiensis subespecie israelensis (Bti). Se evaluó la diferencia en el crecimiento y esporulación de ambas cepas a escala laboratorio (0.5-L) observando los efectos de la esterilización, la higienización y la congelación del digestato en la producción de células viables y esporas por Btk y Bti. Después de evaluar los efectos a escala laboratorio y en relación con los experimentos realizados con reactores aislados de 10-L, se estudia el crecimiento de ambas cepas en estos reactores con 1.5 kg de residuo para evitar los incrementos de temperatura, comparándolos con dos tipos de sustrato: digestato y digestato con fracción orgánica de residuos municipales (FORM) (50:50). Paralelamente, se realizaron pruebas de conservación, análisis microbiológico y bioensayos del bioproducto. Con todos los resultados anteriores, las últimas pruebas realizadas en esta tesis fueron la optimización de la extracción y caracterización de la toxina producida por Bt a partir del cristal proteico, concentrado y conservado a partir de técnicas como: la disrupción, y la liofilización, para conservar el material, donde se observó un 86 y 89% de recuperación de las esporas para Btk y Bti. Se realizó también una purificación del sustrato, para la determinación de las proteínas con efecto biopesticida caracteristicas de Bt a partir de una electroforesis. Esta tesis es un paso adelante para comprobar las posibilidades de obtención del cristal proteico de Bt con efecto biopesticida a partir de FES y poder utilizarlo valorizando los residuos del tratamiento de la fracción orgánica de residuos municipales.
This thesis is part of the DECISIVE project, funded by the European program H2020 (grant agreement No. 689229), which aims to propose and test a decentralized management scheme for the recovery of municipal organic waste. The general objective of this research is the production of value-added bioproducts using digestate of the organic fraction of municipal solid waste as a substrate through solid-state fermentation (SSF). The main bioproduct to explore is a biopesticide inoculating the digestate with Bacillus thuringiensis (Bt), which is known to produce a protein with biopesticidal properties. Firstly, laboratory scale tests were performed to verify the suitability of the digestate to obtain different bioproducts (cellulases, sophorolipids, proteases and biopesticides), achieving the best production results for the biopesticide produced by Bt. These experiments were performed with sterile digestate and hygienized digestate, observing that Bt was able to grow and sporulate using hygienized digestate. Subsequently, a series of operational strategies were studied in thermally insulated 10-L reactors using hygienized digestate. The same process was scaled to a 100-L stirred packed bed reactor without thermal insulation observing a sporulation of 89% with a spore growth 3 times greater than the initial value. Subsequently, a comparison was made between two strains of Bt, Bacillus thuringiensis subspecies kurstaki (Btk) and Bacillus thuringiensis subspecies israelensis (Bti). The difference among both strains at laboratory scale 0.5-L was evaluated observing the effects of sterilization, sanitation and freezing of digestate in the production of viable cells and spores by Btk and Bti. At the same time, conservation tests, microbiological analysis and digestate bioassays were performed. With all the previous results, the last analysis carried out in this thesis was the optimization of the extraction and characterization of the toxin produced by Bt from the protein crystal. Different techniques were applied to concentrate and preserve the protein crystal. The first one was a disruption where viable cells were decreased and spore production increased, releasing the protein crystal. The second one was a lyophilization to conserve the material, where 86 and 89% recovery of spores was observed for Btk and Bti. This thesis is a step forward to verify the characteristics of the Bt protein crystal with biopesticidal effect obtained through SSF and to be able to use the final product valorising the residues of the treatment of the organic fraction of municipal solid waste.
4
Deravel, Jovana. "Caractérisation des lipopeptides d’origine non ribosomique comme biopesticides." Thesis, Lille 1, 2011. http://www.theses.fr/2011LIL10126.
Full textAbstract:
Le premier objectif de ce travail a été d’étudier l’implication des lipopeptides de Bacillus spp. dans la colonisation de la rhizosphère de tomates. Alors que seules les souches produisant de la surfactine sont capables de coloniser un milieu synthétique, toutes les souches testées, colonisent la rhizosphère de tomates avec une plus ou moins bonne efficacité quelque soit leur propriété de production de lipopeptide(s). L’efficacité de la colonisation des racines de tomates est principalement espèce-dépendante. Ce n’est que quand une souche est déjà une bonne colonisatrice que la surfactine semble améliorer cette propriété. Le deuxième objectif a été de tester l’effet des lipopeptides surfactine et mycosubtiline contre le phytopathogène obligatoire de la laitue : Bremia lactucae. À l’échelle du laboratoire, la mycosubtiline à 100 mg/L réduit le pourcentage de plantes infestées de 70 %. La surfactine ne montre aucun effet contre le champignon. Un mélange de mycosubtiline et de surfactine à 50 mg/L chacun diminue le pourcentage de plantes infestées de 65 %. Il semble diminuer le nombre de spores par plante infestée alors que cette propriété n’est pas remarquée avec les autres traitements. L’utilisation de la mycosubtiline dans une serre de culture limite la maladie aux symptômes les moins sévères et protège les plantes saines d’une contamination croisée. L’action des lipopeptides dans la colonisation des racines par Bacillus spp. n’avait jamais été validée in situ. De même, c’est la première fois que l’activité des lipopeptides est testée contre un phytopathogène obligatoireThe first aim of this work was to study the role played by the lipopeptides of Bacillus spp. in the colonization of the tomato rhizosphere. While only the strains producing surfactin are able to colonize a synthetic agar medium, all the strains are able to colonize the rhizosphere of tomatoes with a more or less good efficiency, whatever the lipopeptide(s) they have the capability to produce. The efficiency of the colonization of the tomato rhizosphere is species-dependant. However, surfactine seems to improve the efficiency of only the good colonizing strains. The second aim of this thesis was to test the effect of surfactin and mycosubtilin against a biotrophic parasite of lettuce: Bremia lactucae. Used at 100 mg/L, mycosubtilin reduces the percentage of infested plants of 70 %. Surfactin does not have effect against the fungy. A mixture of mycosubtilin and surfactin at both 50 mg/L decreases the percentage of infested plants of 65 %. This mixture seems to reduce the number of spore per infested plant while this property was not found for the other treatments. The use of mycosubtilin in a greenhouse confines the disease to the lowest classes of severity and protects the healthy plants from a cross contamination.The efficiency of lipopeptides of Bacillus spp. in root colonization by these bacteria was never tested in situ before. Furthermore, this is the first time that the activity of lipopeptides is validated against an obligate phytopathogen
5
Lintz, Julie. "Caractérisation fonctionnelle et valorisation de peptides de défense multifonctionnels chez les arbres de la famille des Salicacées." Electronic Thesis or Diss., Université de Lorraine, 2023. http://www.theses.fr/2023LORR0151.
Full textAbstract:
Les plantes possèdent un système immunitaire capable de stopper la progression des agents pathogènes et de prévenir l'apparition des maladies. Ce système immunitaire utilise des peptides de défense pouvant avoir deux fonctions distinctes: antimicrobiennes ou élicitrices. Rust Induced Secreted Peptide from Populus trichocarpa (PtRISP1) est un peptide du peuplier qui présente à la fois une activité antimicrobienne envers les champignons de l'ordre des Pucciniales et une activité élicitrice des réponses immunitaires chez le peuplier. Les objectifs de la thèse étaient de caractériser le mode d'action antifongique et éliciteur de PtRISP1 et de ses homologues et d'entreprendre des démarches de valorisation de ces peptides comme composés anti-pucciniales. Cette thèse comporte cinq chapitres : un chapitre d'introduction, trois chapitres de résultats, et un chapitre de discussion. Le Chapitre I présente une synthèse bibliographique portant sur l'immunité des plantes, les peptides de défense, les Salicacées, les Pucciniales, et le peptide PtRISP1. Le Chapitre II présente des résultats concernant l'activité élicitrice des peptides RISP. Il montre notamment qu'au sein des génomes des peupliers et des saules, les gènes RISP sont systématiquement regroupés avec des gènes codant pour des récepteurs membranaires de type Leucine Rich Repeat Receptor Proteins (LRR-RP), que nous avons appelés RISP-Associated LRR-RPs (RALR), et que les RALR reconnaissent les peptides RISP pour induire la signalisation immunitaire. Le Chapitre III présente des résultats concernant l'activité antimicrobienne de PtRISP1. Il montre notamment que PtRISP1 cible les extrémités apicales des urédospores de Melampsora larici-populina via des régions protéiques chargées, a une activité spécifique envers les Pucciniales, et s'attache à la surface des feuilles. Le Chapitre IV présente une analyse préliminaire de la famille RISP, qui montre que des membres divergents de la famille présentent des activités anti-Pucciniales et des propriétés biophysiques similaires à PtRISP1. Enfin, le Chapitre V discute les résultats de la thèse en les replaçant dans un contexte plus large et met en perspective la valorisation des peptides végétaux en agriculture. Pour conclure, ces travaux de thèse ont mis en évidence que les peptides RISP sont des phytocytokines reconnues par des récepteurs immunitaires spécifiques, possèdent une activité antimicrobienne spécifique envers les Pucciniales, et ont évolué conjointement avec leurs récepteurs chez les arbres de la famille des SalicacéesPlants have an immune system able to stop the progress of pathogens and prevent the development of disease. This immune system uses defense peptides that can have two distinct functions: antimicrobial or elicitor. Rust Induced Secreted Peptide from Populus trichocarpa (PtRISP1) is a peptide from poplar that exhibits both antimicrobial activity against fungi of the order Pucciniales and elicitor activity in poplar. The objectives of the thesis were to characterize the antifungal and elicitor mode of action of PtRISP1 and its homologues, and to initiate steps to valorize these peptides as anti-pucciniales compounds. This thesis comprises five chapters: an introduction, three results chapters and a discussion chapter. The Chapter I presents a synthesis of the literature on plant immunity, defense peptides, Salicaceae, Pucciniales and the PtRISP1 peptide. Chapter II presents results concerning the elicitor activity of RISP peptides. In particular, it shows that within the poplar and willow genomes, RISP genes are systematically clustered with genes encoding Leucine Rich Repeat Receptor Proteins (LRR-RP), which we have called RISP-Associated LRR-RPs (RALRs), and that RALRs recognize RISP peptides to induce immune signaling. The Chapter III presents the results concerning the antimicrobial activity of PtRISP1. In particular, it shows that PtRISP1 targets the apical tips of Melampsora larici-populina urediniospores via charged protein regions, has specific activity towards Pucciniales, and attaches to leaf surfaces. The Chapter IV presents a preliminary analysis of the RISP family, showing that divergent family members exhibit anti-Pucciniales activities and biophysical properties similar to PtRISP1. Finally, the Chapter V discusses the results of the thesis, placing them in a more wider context and putting the valorization of plant peptides in agriculture into perspective. In conclusion, this thesis work has demonstrated that RISP peptides are phytocytokines recognized by specific immune receptors, have a specific antimicrobial activity towards Pucciniales, and have co-evolved with their receptors in trees of the Salicaceae family
6
Ozcan, Orhan. "Medium Development For Production Of Bacillus Thuringiensis Based Biopesticides." Master's thesis, METU, 2008. http://etd.lib.metu.edu.tr/upload/3/12609285/index.pdf.
Full textAbstract:
The insect pathogen Bacillus thuringiensis (Bt) holds great promise as an effective and friendly way for management of the pests with safety for nontarget animals and humans. However, high capital investment due to high production and formulation cost of commercial Bt preparations has caused prohibitive effect on companies. The present study mainly aimed at developing a low cost medium that supports the growth of different Bt strains and their specific bioinsecticidal δ
-endotoxins (crystal proteins). A comparison was made between the representative members of three different subspecies of Bt to observe toxin yields in response to certain nutritional conditions. Three different Bt subspecies were Bt kurstaki (strain 81), Bt israelensis (strain HD500) and Bt tenebrionis (strain 3203), producing lepidoptera- and diptera-specific Cry1 and Cry2, diptera-specific Cry4Ba and Cry11Aa and coleoptera-specific Cry3Aa toxins, respectively. Studies were conducted to optimize glucose and inorganic phosphate concentrations in standard DSM medium for the production of these Bt-based biopesticides. General suppression of toxin yields in high glucose medium (10 g/L) thought the generality of carbon catabolite regulation for biosynthesis of different types of toxins. Inorganic phosphate (Pi) level was important for Cry4Ba, Cry11Aa and Cry3Aa biosynthesis while Cry1 and Cry2 production was not responsive to high Pi. Wastewater sludge, fruit residues and broiler litter were next tested as cheap raw materials for Bt-based biopesticide production in batch cultures. Broiler litter seemed to be a much better substrate among all since some degree of production of each toxin was observed at almost every stage of fermentation. The processing of broiler litter was found to significantly improve toxin yields. The medium prepared from processed broiler litter was successfully used to cultivate all Bt stains and obtain bioinsecticidal proteins in high yields which were comparable or higher than those that can be obtained on standard semi-synthetic media.
7
Wilson, Jonathan Alexander. "Novel downstream processing for recovery of nematodes for biopesticides." Thesis, University College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.404895.
Full text
8
Besset-Manzoni, Yoann. "Améliorer les principes de sélection de nouveaux agents bactériens de biocontrôle contre la fusariose du blé." Thesis, Lyon, 2018. http://www.theses.fr/2018LYSE1124/document.
Full textAbstract:
Pour lutter contre les nuisibles des cultures (herbivores et pathogènes), de nouvelles voies ont été explorées et en particulier la lutte biologique. Utilisé la nature et ses organismes pour réguler les populations de pathogènes, tel est le but de la lutte biologique aussi appelé biocontrôle. Parmi les organismes couramment retrouvés dans le biocontrôle se trouve les micro-organismes, que cela soit des champignons ou des bactéries. Ces micro-organismes possèdent des capacités très intéressante pour des agriculteurs et agronomes. En effet, des études ont montré qu’ils étaient capables d’interagir avec les plantes pour permettre une meilleure croissance et santé de celle-ci. Alors, dans cette thèse, nous nous sommes intéressés aux bactéries pour trouver des méthodes de luttes alternatives à Fusarium graminearum, un pathogène des céréales responsables de fortes pertes de rendements, en particulier grâce à la production de mycotoxines qui va rendre les grains impropres à la consommation humaine et animales. Par l’intermédiaire d’une approche original consistant à garder des souches non-inhibitrice in vitro pour des tests in planta, nous avons pu montrer les limites de la sélection in vitro. Grâce à l’exploration métaboliques d’une souche particulièrement efficace, nous avons aussi pu mettre en évidence de potentiels nouvelles molécules antifongiques. Par l’intermédiaire d’une souche modèle, nous avons explorer les mécanismes de la mise en place d’une résistance systémique chez le blé induite par les bactéries. Et enfin, nous avons exploré le potentiel des combinaisons de bactéries dans la protection du blé qui semble représenter un vrai futur dans le monde du biocontrôle. Les travaux effectués s’inscrivent dans les besoins de nouvelles ressources pour limiter l’utilisation des pesticides, mais aussi dans un besoin de mieux comprendre les interactions tripartite entre blé, pathogène et bactéries bénéfiquesTo combat pests of crops (herbivores and pathogens) new pathways have been explored, in particular biological control. Used the nature and its organisms to regulate the populations of pathogens, that is the goal of the biological fight also called biocontrol. Among the organisms commonly found in biocontrol are micro-organisms, be they fungi or bacteria. These microorganisms have very interesting capabilities for farmers and agronomists. Indeed, studies have shown that they are able to interact with plants to allow a better growth and health of it.Then, in this thesis, we were interested in bacteria to find alternative methods of struggle with Fusarium graminearum, a pathogen of cereals responsible for high yield losses, especially thanks to the production of mycotoxins that will make the seeds unfit for human and animal consumption.Through an original approach of keeping noninhibitory strains in vitro for in planta tests, we have been able to show the limitations of in vitro selection. Thanks to the metabolic exploration of a particularly efficient strain, we have also been able to highlight potential new antifungal molecules. Through a model strain, we explored the mechanisms of the establishment of systemic resistance in wheat induced by bacteria. And finally, we have explored the potential of bacterial combinations in wheat protection that seems to represent a real future in the world of biocontrol.The work done is in line with the needs of new resources to limit the use of pesticides, but also in a need to better understand the tripartite interactions between wheat, pathogen and beneficial bacteria
9
Mejias, Torrent Laura. "A step towards biowaste digestate valorization: process development for bt-derived biopesticides production through ssf and performace at demonstration scale." Doctoral thesis, Universitat Autònoma de Barcelona, 2020. http://hdl.handle.net/10803/671265.
Full textAbstract:
Dins el marc del projecte europeu DECISIVE (grant agreement No 689229) aquesta tesi es centra en la valorització del digestat obtingut de la gestió descentralitzada de la fracció orgànica dels residus municipals (FORM) mitjançant la tecnologia de fermentació en estat sòlid (FES). Treballs anteriors enfocats a la prova de concepte van destacar la viabilitat de produir biopesticides derivats de Bacillus thuringiensis (Bt) utilitzant digestat com a principal substrat.
Partint d’;aquests resultats, es van realitzar una sèrie de dissenys d’;experiments a escala laboratori (0.5-L) per determinar els paràmetres amb més rellevància en la fermentació. La temperatura i l’;addició de FORM com a co-substrat es van identificar com a paràmetres clau del procés. Aquest efecte es va estudiar a una escala superior (1.6-L), confirmant la necessitat d’;afegir co-substrat per tal de millorar els rendiments de producció. En aquest punt, també es va identificar i destacar la importància dels nivells d’;oxigen durant les primeres hores de procés. Com a resultant, es va desenvolupar una estratègia d’;aeració per tal de maximitzar la producció d’;espores.
Aquesta estratègia va ser validada a un reactor prototip (22-L), utilitzant dos soques diferents: Bt var. kurstaki i Bt var. israelensis. Els resultats van ser molt prometedors quan el procediment es realitzava en batch, però la producció final es veia reduïda significativament quan es treballava en fed-batch o batchs seqüencials.
Finalment, aquesta estratègia de producció es va implementar al reactor pilot de 290-L, intentant aconseguir l’;ambient favorable per incrementar el creixement i esporulació de Bt. La qualitat del material fermentat va ser analitzada en termes de concentració d’;espores, maduresa del sòlid, i identificació i quantificació de microplàstics.
Aquest projecte ha rebut finançament del programa de recerca i innovació Horizon 2020 de la Unió Europea segons el Grant Agreement No 689229.Dentro del marco del proyecto europeo DECISIVE (grant agreement No 689229) esta tesis se centra en la valorización del digestato obtenido de la gestión descentralizada de la fracción orgánica de los residuos municipales (FORM) mediante la tecnología de fermentación en estado sólido (FES). Trabajos anteriores enfocados a la prueba de concepto destacaron la viabilidad de producir biopesticidas derivados de Bacillus thuringiensis (Bt) utilizando digestato como principal sustrato. A partir de estos resultados, se realizaron una serie de diseños de experimentos a escala laboratorio (0.5-L) con el objetivo de determinar los parámetros con más relevancia en la fermentación. La temperatura y la adición de FORM como co-sustrato se identificarion como parámetros clave del proceso. Su efecto se estudió a escala superior (1.6-L), confirmando la necesidad de añadir co-sustrato para mejorar los rendimientos de producción. En este punto, también se identificó y destacó la importancia de los niveles de oxígeno durante las primeras horas de proceso. Como resultado, se desarrolló una estrategia de aeración para maximizar la producción de esporas. Esta estrategia se validó en un reactor prototipo (22-L), utilizando dos cepas diferentes: Bt var. kurstaki y Bt var. israelensis. Los resultados fueron muy prometedores cuando el proceso operaba en bach, pero la producción final disminuía significativamente cuando se operaba en fed-batch o batch secuencial. Finalmente, esta estrategia de producción se implementó en el reactor piloto de 290-L, intentado lograr en ambiente favorable para incrementar el crecimiento y esporulación de Bt. Se estudió la calidad del sólido fermentado en referencia a la concentración de esporas, madurez del sólido, e identificación y cuantificación de microplásticos. Este proyecto ha recibido fondos del programa de investigación e innovación Horizon 2020 de la Unión Europea según el Grant Agreement No 689229.
In the framework of the European project DECISIVE (grant agreement No 689229) the present thesis is focused on the valorization of digestate from the decentralized management of the organic fraction of the municipal solid waste through the solid-state fermentation (SSF) technology. Previous work focused on the proof of concept of the idea highlighted the viability of producing Bacillus thuringiensis-derived biopesticides using digestate as a principal substrate. From these results, a first assessment at a laboratory scale (0.5-L) using the design of experiments’ methodology was performed for determining the more relevant parameters in the fermentation. Temperature and the use of biowaste as co-substrate were identified as key parameters for the process. This effect was studied at 1.6-L, confirming the need of adding co-substrate for increasing the production yields. At that point, the relevance of oxygen levels in the firsts hours of fermentation was identified and highlighted. As a result, an aeration strategy was developed with the aim of maximizing the spore production. This strategy was validated at a prototype reactor (22-L) using two different strains: Bt var. kurstaki and Bt var. israelensis. Promising results were observed when the process was performed on batch mode. However, the final production was significantly reduced when working on fed-batch or sequential batch mode. Lastly, the developed operation strategy was implemented at the 290-L pilot reactor, trying to achieve an adequate environment for boosting Bt growth and sporulation. The quality of the fermented material was assessed in terms of spore concentration, solid maturity, and microplastics identification and quantification. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No 689229.
10
Selim, Sameh. "Purification et caractérisation de la paenimyxine, un bio-pesticide produit par la bactérie tellurique, Paenibacillus sp. Souche B2 : étude de son impact sur des microorganismes du sol et les réactions de défense de la plante." Dijon, 2005. http://www.theses.fr/2005DIJOMU09.
Full textAbstract:
Dans ce travail, un facteur antagoniste produit par Paenibacillus sp. Souche B2 a été purifié. Ce facteur montre un large spectre d'action vis-à-vis de bactéries Gram- et Gram+, ainsi que des champignons pathogènes. Ce facteur est constitué de trois peptides antagonistes, nous avons proposé de les grouper sous le nom du "paenimyxine". Après un traitement du sol avec la paenimyxine, une réduction transitoire du nombre de bactéries cultivables, ainsi de leur structure génétique, a été observée entre 2 et 4 jours après le traitement. Après une incubation des racines de plantules de Medicago Truncatula avec 1,23uM de paenimyxine pendant 24 h, avant l'inoculation avec Fusarium acuminatum, 95% des symptômes nécrotiques sont supprimés. L'expression de 64 gènes connus pour être liés aux mécanismes de défense a été étudié par Northern inverse, et l'activation de l'expression des gènes a été confirmée par RT-PCR semi quantitative. La démonstration a été ainsi apportée pour la première fois que des molécules produites par une bactérie antagoniste, sont capables d'induire chez la plante une résistance vis-à-vis d'un champignon pathogène des racines.
Books on the topic "Biopesticides"
1
Hall, Franklin R., and Julius J. Menn. Biopesticides. New Jersey: Humana Press, 1998. http://dx.doi.org/10.1385/0896035158.
Full text
2
Deshmukh, A. M. biopesticides used for nyctanthus Biofertilizers and biopesticides. Jaipur: Technoscience Publications, 1998.
Find full text
3
Opender, Koul, and Dhaliwal G. S, eds. Microbial biopesticides. London: Taylor & Francis, 2002.
Find full text
4
Deshpande, M. V. Novel biopesticides. New Delhi: National Institute of Science Communication (CSIR), 1998.
Find full text
5
Opender, Koul, and Dhaliwal G. S, eds. Phytochemical biopesticides. Amsterdam, The Netherlands: Harwood Academic, 2001.
Find full text
6
Nollet, Leo M. L., and Showkat Mir. Biopesticides Handbook. 2nd ed. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003265139.
Full text
7
Glare, Travis R., and Maria E. Moran-Diez, eds. Microbial-Based Biopesticides. New York, NY: Springer New York, 2016. http://dx.doi.org/10.1007/978-1-4939-6367-6.
Full text
8
Suri, Shalini. Biofertilizers and biopesticides. New Delhi: A.P.H. Pub. Corp., 2011.
Find full text
9
S, Parmar B., and Devakumar C, eds. Botanical and biopesticides. New Delhi: Westvill Publishing House, 1993.
Find full text
10
Rajeshwari, R., and Vikram Appanna. Biopesticides in Horticultural Crops. London: CRC Press, 2021. http://dx.doi.org/10.1201/9781003243427.
Full textBook chapters on the topic "Biopesticides"
1
Mehrotra, Sudhir, Shiv Kumar, Mohd Zahid, and Minal Garg. "Biopesticides." In Principles and Applications of Environmental Biotechnology for a Sustainable Future, 273–92. Singapore: Springer Singapore, 2016. http://dx.doi.org/10.1007/978-981-10-1866-4_8.
Full text
2
Saha, Sujoy, Ratna Thosar, Sumant Kabade, Komal Pawar, and Kaushik Banerjee. "Biopesticides." In Trajectory of 75 years of Indian Agriculture after Independence, 675–703. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-7997-2_25.
Full text
3
Nollet, Leo M. L. "Biopesticides." In Biopesticides Handbook, 3–6. 2nd ed. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003265139-2.
Full text
4
Holmes, K., M. Chaudhary, D. Babendreier, M. Bateman, J. Grunder, M. Mulaa, L. Durocher-Granger, and M. Faheem. "Selecting biopesticides." In Biopesticides manual: guidelines for selecting, sourcing and using biocontrol agents for key pests of tobacco, 5–35. Wallingford: CABI, 2018. http://dx.doi.org/10.1079/9781789242027.0005.
Full text
5
Holmes, K., M. Chaudhary, D. Babendreier, M. Bateman, J. Grunder, M. Mulaa, L. Durocher-Granger, and M. Faheem. "Sourcing biopesticides." In Biopesticides manual: guidelines for selecting, sourcing and using biocontrol agents for key pests of tobacco, 36–102. Wallingford: CABI, 2018. http://dx.doi.org/10.1079/9781789242027.0036.
Full text
6
Srivastava, Nidhi, Usha Kumari, and Jai Kumar. "Microbial Biopesticides." In Microbial Products, 189–203. Boca Raton: CRC Press, 2022. http://dx.doi.org/10.1201/9781003306931-15.
Full text
7
Wakefield, M. E. "Microbial Biopesticides." In Recent Advances in Stored Product Protection, 143–68. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-56125-6_7.
Full text
8
Sharma, N., A. S. Bhandari, and P. K. Shukla. "Entomopathogenic Biopesticides." In Bio-management of Postharvest Diseases and Mycotoxigenic Fungi, 121–44. First edition. | Boca Raton, FL : CRC Press, 2021. |: CRC Press, 2020. http://dx.doi.org/10.1201/9781003089223-7.
Full text
9
Haroon, Zareeka, Hyda Haroon, Asfia Shabbir, Irzam Haroon, and Yawar Sadiq. "Microbial Biopesticides." In Biopesticides Handbook, 153–61. 2nd ed. Boca Raton: CRC Press, 2023. http://dx.doi.org/10.1201/9781003265139-13.
Full text
10
Meena, Poonam, Neelam Poonar, Sampat Nehra, and P. C. Trivedi. "Applications of Microbial Biopesticides." In Industrial Applications of Soil Microbes, 85–101. BENTHAM SCIENCE PUBLISHERS, 2023. http://dx.doi.org/10.2174/9789815050264123020008.
Full textAbstract:
Microbial biopesticides involve various microorganisms such as bacteria, fungi, viruses, nematode-associated bacteria, protozoans, and endophytes working against invertebrate pathogens in agro-ecosystems. Such novel biopesticidal products, after extensive research work, have been explored in the global market to combat synthetic pesticide application adverse problems. Recent academic and industrial efforts are involved in the discovery of toxins and virulence factors from microbial species for the synthesis of commercial formulations. The current review is the expansion of the application of various bacteria, fungi, viruses, nematodes, protozoans, and endophytes for biopesticide formulations and their role in pest management.
Conference papers on the topic "Biopesticides"
1
Smagghe, Guy. "RNAi-based biopesticides." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.93500.
Full text
2
Gatehouse, John. "Fusion protein-based biopesticides." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.93497.
Full text
3
Stingaci, Aurelia, and Leonid Volosciuc. "Isolate locale ale baculovirului entomopatogenic ca o tehnologie de formulare inovatoare, care protejează biopesticidul din degradare a radiației ultraviolete." In VIIth International Scientific Conference “Genetics, Physiology and Plant Breeding”. Institute of Genetics, Physiology and Plant Protection, Republic of Moldova, 2021. http://dx.doi.org/10.53040/gppb7.2021.91.
Full textAbstract:
This paper presents the conceptual conceptual vision a formulation technology for biopesticides in which the active ingredient (baculovirus) is an active coal. Importantly, this indgredient protects the sen-sitive viral DNA from degrading in sunlight, but dissolves in the alkaline insect gut to release the virus, which then infects and kills the pest. We show, using this ingredient, in both laboratory bioassays and field tests, that this can extend the efficacy of the biopesticide well beyond the few hours of existing virus formulations, potentially increasing the spray interval and reducing the need for high application rates. Are presented both theoretical foundations and practical applications and described the results oriented for implementation and functionality of organic agriculture in Republic of Moldova.
4
Stingaci, Aurelia, and Leonid Volosciuc. "Particularitățile identificării VPN și VG a Hyphantria Cunea prin aplicarea microscopiei optice și electronice." In International symposium ”Functional ecology of animals” dedicated to the 70th anniversary from the birth of academician Ion Toderas. Institute of Zoology, Republic of Moldova, 2019. http://dx.doi.org/10.53937/9789975315975.61.
Full textAbstract:
Biopesticides are mass-produced, biologically based agents used for the control of plant pests. They are an important part of integrated pest management (IPM), which aims to use complementary methods to manage pest populations at low levels, rather than eliminate them entirely. Biopesticides are being used on increasing scales and there is considerable interest in their potential as alternatives to conventional pesticides. Biopesticides have also attracted great interest in the international research community, with a significant increase in the number of publications devoted to the subject. At Institute of Genetics, Physiology and Plant Protection are prepared the bioinsecticides for use in Republic Moldova, mostly for the control. In order to reduce the population of insect it is recommended utilization of inoffensive preparations baculoviruses highlypathogenic for the leaf-champing vermis of the Hyphantria cunea, were selected from the insect natural populations which is an efficient preparation for combating this pest in agricultural, onamental and forest biocenosis. This study aimed to highlight new agents for biological control of pest. The results of the present study revealed the larvicidal potential of baculovirusess isolates found in the larvae of H. cunea, local production of biopesticides, which will reduce the final cost of the product and will more accessible to farmer.
5
Butt, Tariq. "Biopesticides — innovative technologies and strategies for pest control." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.95056.
Full text
6
Malinga, Lawrence Nkosikhona. "Efficacy of biopesticides for pest control in cotton." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.106665.
Full text
7
Essiedu, Justice A., Feyisayo O. Adepoju, and Maria N. Ivantsova. "Benefits and limitations in using biopesticides: A review." In THE VII INTERNATIONAL YOUNG RESEARCHERS’ CONFERENCE – PHYSICS, TECHNOLOGY, INNOVATIONS (PTI-2020). AIP Publishing, 2020. http://dx.doi.org/10.1063/5.0032223.
Full text
8
Ghosh, Saikat Kumar B. "Emerging biopesticides to control insect pests in urban environments." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.94332.
Full text
9
Gatehouse, Angharad M. R. "Biopesticides targeting voltage-gated ion channels: Efficacy and biosafety." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.106496.
Full text
10
LI, Hongmei. "Predicting the efficacy of biopesticides againstLocust migratoriamanilensis in China." In 2016 International Congress of Entomology. Entomological Society of America, 2016. http://dx.doi.org/10.1603/ice.2016.113253.
Full textReports on the topic "Biopesticides"
1
Pilcher, Carol, Martin Chilvers, Travis Faske, Andrew Friskop, Alyssa Koehler, Darren Mueller, Adam Sisson, Darcy Telenko, Albert Tenuta, and Kiersten Wise. Biopesticides for Crop Disease Management. United States: Crop Protection Network, September 2023. http://dx.doi.org/10.31274/cpn-20230919-0.
Full text
2
Richter, E., Patrice Marchand, B. L. Ingegno, L. Tavella, V. A. Vassiliou, and R. J. M. Meijer. How to integrate biopesticides in organic greenhouse growing systems. BioGreenhouse, 2016. http://dx.doi.org/10.18174/373603.
Full text
3
Mejía, Cindy, Johana Sanabria, Ginna Quiroga, Erika Grijalba, and Martha Goméz. Effect of substrate composition and drying process on Metarhizium rileyi Nm017’s conidia quality for control of Helicoverpa zea. Corporación colombiana de investigación agropecuaria - AGROSAVIA, 2019. http://dx.doi.org/10.21930/agrosavia.poster.2019.13.
Full textAbstract:
The drying process is an important step during the production process of biopesticides. Nonetheless, this process causes a stress condition associated to loss of water; resulting a decrease of enzymatic activity, viability, and virulence in case of entomopathogenic fungi. Thus, suitable conditions must be implemented through the entire process to improve desiccation tolerance and keep stable cells. Therefore, the aim of this work was to evaluate several medium compositions in solid-state fermentation (T1, T2, T3, T4, and T5), and determine the e ect on viability, induction of enzymatic activity, and virulence of dried conidia.
4
Lovera, Andrea, Mariano Belaich, Cindy Mejía, Laura Villamizar, Manuel A. Patarroyo,, and Gloria Barrera. Characterization of chitinases of Beauveria bassiana (Bv ) induced in semisolid-state fermentation. Corporación Colombiana de Investigación Agropecuaria - AGROSAVIA, 2012. http://dx.doi.org/10.21930/agrosavia.poster.2012.11.
Full textAbstract:
A major consideration in the development of biopesticides based on entomopathogenic fungi has been the virulence and pathogenicity in terms of mean time of mortality and mean lethal concentration against the target insect. Virulence factors as chitinases degrade insect chitin facilitating the hyphal penetration through the cuticle. In this sense, Beauveria bassiana chitinases are an important cuticle-degrading group of enzymes implicated in the pathogenesis to insects, with high potential to develop biotechnological tools for in pest control. The aim of this study was to characterize chitinases produced by a Colombian strain of B. bassiana (Bv062) isolated from a Diatraea sp. larvae naturally infected in a sugar cane crop.
5
Chejanovsky, Nor, and Bruce D. Hammock. Enhancement of Baculoviruses' Insecticidal Potency by Expression of Synergistic Anti-Insect Scorpion Toxins. United States Department of Agriculture, January 1996. http://dx.doi.org/10.32747/1996.7573070.bard.
Full textAbstract:
The extensive use or non-specific, hazardous, chemical insecticides demands the development of "healthier" alternative means for pest control. Insect-specific, baculoviruses expressing anti-insect toxin genes (from mites or scorpions) demonstrated in laboratory assays and field trials enhanced insecticidal activity and provided some protection from lepidopterous larvae to agricultural plantations. To utilize recombinant baculoviruses as commercial biopesticides in row crop agriculture, further increase in their speed of kill should be achieved and the reduction in crop damage should be comparable to the levels obtained with organic insecticides (the problem). In this project we developed strategies to improve further the efficacy of recombinant baculoviruses which included: I) Synergism among baculoviruses expressing different anti-insect toxins: a) Synergism among two complementary anti-insect scorpion neurotoxins each expressed by a separate recombinant baculovirus, both regulated by the same or a different viral promoter. b) Synergism among two complementary anti-insect scorpion neurotoxins expressed by the same recombinant virus, both regulated by the same or a different viral promoter respectively. The above included two classes of pharmacologically complementary toxins: i) toxins with strictly anti-insect selectivity (excitatory and depressant); ii) toxins with preferential anti-insect activity (anti-insect alpha toxins). c) Synergism among wild type viruses, recombinant baculoviruses and chemicals (insecticides and phytochemicals) II) Identification of more potent toxins against lepidopterous pests for their expression by baculoviruses. Our approach was based on the synergistic effect displayed by the combined application of pairs of anti-insect toxins to blowfly and lepidopterous larvae that resulted in 5 fold increase in their insecticidal activity without apparent increase in their anti-mammal toxicity (toxins LqhIT2 and LqhaIT, LqhIT2 and AaIT, and LqhaIT and AaIT (1). Thus, we developed new concepts and produced a "second generation" of recombinant baculoviruses with enhanced potencies and speeds of kill comparable to classical insecticides. These achievements contribute to make these biopesticides a viable alternative to minimize the use of hazardous chemicals in pest control. Also, our project contributed new tools and model systems to advance the study of insect sodium channels.
6
Cytryn, Eddie, Mark R. Liles, and Omer Frenkel. Mining multidrug-resistant desert soil bacteria for biocontrol activity and biologically-active compounds. United States Department of Agriculture, January 2014. http://dx.doi.org/10.32747/2014.7598174.bard.
Full textAbstract:
Control of agro-associated pathogens is becoming increasingly difficult due to increased resistance and mounting restrictions on chemical pesticides and antibiotics. Likewise, in veterinary and human environments, there is increasing resistance of pathogens to currently available antibiotics requiring discovery of novel antibiotic compounds. These drawbacks necessitate discovery and application of microorganisms that can be used as biocontrol agents (BCAs) and the isolation of novel biologically-active compounds. This highly-synergistic one year project implemented an innovative pipeline aimed at detecting BCAs and associated biologically-active compounds, which included: (A) isolation of multidrug-resistant desert soil bacteria and root-associated bacteria from medicinal plants; (B) invitro screening of bacterial isolates against known plant, animal and human pathogens; (C) nextgeneration sequencing of isolates that displayed antagonistic activity against at least one of the model pathogens and (D) in-planta screening of promising BCAs in a model bean-Sclerotiumrolfsii system. The BCA genome data were examined for presence of: i) secondary metabolite encoding genes potentially linked to the anti-pathogenic activity of the isolates; and ii) rhizosphere competence-associated genes, associated with the capacity of microorganisms to successfully inhabit plant roots, and a prerequisite for the success of a soil amended BCA. Altogether, 56 phylogenetically-diverse isolates with bioactivity against bacterial, oomycete and fungal plant pathogens were identified. These strains were sent to Auburn University where bioassays against a panel of animal and human pathogens (including multi-drug resistant pathogenic strains such as A. baumannii 3806) were conducted. Nineteen isolates that showed substantial antagonistic activity against at least one of the screened pathogens were sequenced, assembled and subjected to bioinformatics analyses aimed at identifying secondary metabolite-encoding and rhizosphere competence-associated genes. The genome size of the bacteria ranged from 3.77 to 9.85 Mbp. All of the genomes were characterized by a plethora of secondary metabolite encoding genes including non-ribosomal peptide synthase, polyketidesynthases, lantipeptides, bacteriocins, terpenes and siderophores. While some of these genes were highly similar to documented genes, many were unique and therefore may encode for novel antagonistic compounds. Comparative genomic analysis of root-associated isolates with similar strains not isolated from root environments revealed genes encoding for several rhizospherecompetence- associated traits including urea utilization, chitin degradation, plant cell polymerdegradation, biofilm formation, mechanisms for iron, phosphorus and sulfur acquisition and antibiotic resistance. Our labs are currently writing a continuation of this feasibility study that proposes a unique pipeline for the detection of BCAs and biopesticides that can be used against phytopathogens. It will combine i) metabolomic screening of strains from our collection that contain unique secondary metabolite-encoding genes, in order to isolate novel antimicrobial compounds; ii) model plant-based experiments to assess the antagonistic capacities of selected BCAs toward selected phytopathogens; and iii) an innovative next-generation-sequencing based method to monitor the relative abundance and distribution of selected BCAs in field experiments in order to assess their persistence in natural agro-environments. We believe that this integrated approach will enable development of novel strains and compounds that can be used in large-scale operations.
7
Audsley, Neil, Gonzalo Avila, Claudio Ioratti, Valerie Caron, Chiara Ferracini, Tibor Bukovinszki, Marc Kenis, et al. Nun moth, Lymantria monacha (L.). Euphresco, 2023. http://dx.doi.org/10.1079/20240228589.
Full textAbstract:
The nun moth (Lymantria monacha) is a significant pest of conifer trees, particularly pine and spruce, across its native range from Western Europe to the Russian Far East and China. It experiences periodic outbreaks and overwinters in the egg stage. Although it is not yet invasive outside its native range, it has not been the target of classical biological control. The most promising natural enemies for potential control include the tachinid fly Parasetigena sylvestris, which is specific to Lymantria spp. and a key parasitoid of the gypsy moth in North America, and the nuclear polyhedrosis virus (NPV), which is specific to the nun moth and has been developed as a biopesticide. Other potential biological control agents include the braconid Cotesia melanoscela and the ichneumonids Casinaria scutelllaris and Elachertus charondas, although their specificity and effectiveness need further investigation. In the Russian Far East, the parasitoids Parasetigena sylvestris and Blepharipa schineri are noted, with the latter requiring further study due to possible specificity differences.
8
Biopesticides and Their Regulation in Malaysia. Food and Fertilizer Technology Center for the Asian and Pacific Region, November 2022. http://dx.doi.org/10.56669/ibqe2658.
Full text
9
Formulation, Application and Commercialization of Biopesticides in India. Food and Fertilizer Technology Center for the Asian and Pacific Region, November 2022. http://dx.doi.org/10.56669/kshw3457.
Full text
10
Study on the Development of New Biopesticide to Control Pathogen Fungi and Nematode Damaged Black Pepper in Vietnam. Food and Fertilizer Technology Center for the Asian and Pacific Region, May 2023. http://dx.doi.org/10.56669/gvcb6290.
Full text