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1

Magnaguagno, Maurício C., and Felipe Meneguzzi. "BioPlan: Classical Planning with Crowd simulation." Journal on Interactive Systems 8, no. 1 (September 14, 2017): 1. http://dx.doi.org/10.5753/jis.2017.674.

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Crowd simulation for evacuation situations often assumes that all agents are trying to reach a single point within an environment. Although such an assumption is not entirely wrong, human agents often exhibit more complex behaviors, even if deviations from the standard behavior are not particularly frequent. Classical planning is far from the best way to achieve the minimal path or correct behavior for agents, but adds a deeper level of reasoning about complex goal-achievement and about actions that are more complex than simply moving about. In this paper, we describe a crowd simulation experiment that uses classical AI planning to enrich the behavior of the agents in the scenario. Using this approach, we can express not only the target destination of the agents, but also (sub)goals and path preferences.
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Sanchez-Nieto, B., and A. E. Nahum. "Bioplan: software for the biological evaluation of radiotherapy treatment plans." Medical Dosimetry 25, no. 2 (March 2000): 71–76. http://dx.doi.org/10.1016/s0958-3947(00)00031-5.

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3

Figueira, A., and F. Ponte. "487 TCP and NTCP calculation using BIOPLAN and ALBIREO TARGET ? a comparison study." Radiotherapy and Oncology 76 (September 2005): S209—S210. http://dx.doi.org/10.1016/s0167-8140(05)81463-x.

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Pour, Noushin Hassan, Alireza Farajollahi, Masoud Jamali, Ahad Zeinali, and Amir Ghasemi Jangjou. "Comparison of three and four-field radiotherapy technique and the effect of laryngeal shield on vocal and spinal cord radiation dose in radiotherapy of non-laryngeal head and neck tumors." Polish Journal of Medical Physics and Engineering 24, no. 1 (March 1, 2018): 25–31. http://dx.doi.org/10.2478/pjmpe-2018-0004.

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AbstractIntroduction: Due to the effect of radiation on both the tumor and the surrounding normal tissues, the side effects of radiation in normal tissues are expected. One of the important complications in the head and neck radiotherapy is the doses reached to the larynx and spinal cord of patients with non-laryngeal head and neck tumors.Materials and Methods: In this study, CT scan images of 25 patients with non-laryngeal tumors including; lymph nodes, tongue, oropharynx and nasopharynx were used. A three-field and a four-field treatment planning with and without laryngeal shield in 3D CRT technique were planned for each patient. Subsequently, the values of Dmin, Dmean, Dmax and Dose Volume Histogram from the treatment planning system and NTCP values of spinal cord and larynx were calculated with BIOPLAN and MATLAB software for all patients.Results: Statistical results showed that mean values of doses of larynx in both three and four-field methods were significantly different between with and without shield groups. Comparison of absorbed dose didn’t show any difference between the three and four field methods (P>0.05). Using Shield, just the mean and minimum doses of spinal cord decreased in both three and four fields. The NTCP of the spinal cord and larynx by three and four-field methods with shield in the LKB and EUD models significantly are less than that of the three and four fields without shields, and in the four-field method NTCP of larynx is less than three radiation field.Conclusion: The results of this study indicate that there is no significant difference in doses reached to larynx and spinal cord between the treatments techniques, but laryngeal shield reduce dose and NTCP values in larynx considerably.
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Hlinková, Eva-Žofie, Zdeněk Varga, and Jana Zábranská. "Biologická konverze energeticky bohatých plynů na biometan." Entecho 4, no. 1 (June 30, 2021): 1–9. http://dx.doi.org/10.35933/entecho.2021.001.

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Přechod ze stávajících neobnovitelných zdrojů elektrické energie na zdroje obnovitelné se jeví jako vhodné řešení pro celosvětově narůstající spotřebu energie. Udržitelná technologie pro zpracování organických odpadů formou anaerobní fermentace produkuje bioplyn, z kterého se odstraněním oxidu uhličitého získává biometan – energeticky bohatý plyn kompatibilní se zemním plynem a využitelný jako biopalivo. Zaváděním externího vodíku, získaného z přebytečné energie z obnovitelných zdrojů, do procesu anaerobní fermentace dochází pomocí hydrogenotrofních metanogenů k redukci oxidu uhličitého na metan, čímž se zvyšuje výhřevnost bioplynu v ideálním případě až na biometan. V rámci této práce byla zkoumána technologie obohacování bioplynu pomocí vodíku přímým zaváděním do fermentoru tzv. metoda in-situ a s využitím externího bioreaktoru tzv. metoda ex-situ Získané výsledky z provozu laboratorních modelů insitu a ex-situ bioreaktoru poslouží k sestrojení poloprovozního modelu této technologie pro následné převedení do praxe. Abstract - EN The transition from existing sources of electricity to renewables seems to be a suitable solution for the global increase of energy consumption. Sustainable technology of anaerobic fermentation for the treatment of organic wastes produces biogas, from which is by removing carbon dioxide obtained biomethane – energy-rich gas compatible with natural gas and can be used as biofuel. Hydrogen obtained by using excess energy production from renewable sources, can be introduce into the anaerobic fermentation process. Hydrogenotrophic methanogens use external hydrogen for reduction of carbon dioxide to methane, which increases energetical potencial of biogas, ideally up to the level of biomethane. In this work, the technology of enrichment of biogas with hydrogen by direct introduction into the fermenter, the “in-situ” method and with the use of an external bioreactor, the “ex-situ method,” was investigated. The results obtained from the operation of laboratory models of insitu and ex-situ bioreactors will be used to build a pilot model of this technology for subsequent implementation in practice.
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Harboe-Sorensen, R., H. Meijer, J. C. Ronnet, R. Demets, L. Adams, W. Heinrich, and H. Rocher. "BIOPAN-flight experiment "card"." IEEE Transactions on Nuclear Science 41, no. 6 (December 1994): 2340–45. http://dx.doi.org/10.1109/23.340585.

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Сабирова, Разина, Razina Sabirova, Рафил Шакиров, Rafil Shakirov, Закиржан Бикмухаметов, and Zakirzhan Bikmuhametov. "BIOPLANT FLORA - NEW GENERATION FERTILIZER." Vestnik of Kazan State Agrarian University 14, no. 2 (July 29, 2019): 37–42. http://dx.doi.org/10.12737/article_5d3e15f17c3223.64554857.

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The efficiency of Bioplant Flora fertilizer was experimentally proved in increasing the winter wheat productivity of Kazanskaya 560 in the conditions of Middle Volga region. The research was carried out in 2009–2012 years on stationary experiments of Agriculture and agrochemical research Department of the Tatar Scientific Institute of Agriculture in accordance with generally accepted methods. The soil is gray forest, the humus content is 3.0-3.5%, phosphorus and potassium - 250-260 and 80-100 mg/kg, respectively, the amount of absorbed bases is 20-21 mg-eq/100 g, рНсол of arable layer is 5.0 and 5.5. The studies were conducted to study the responsiveness of winter wheat of Kazanskaya 560 variety to the humate fertilizer Bioplant Flora. Bioplant Flora is the newest, nanotechnological fertilizer based on humic acids with trace elements, which was used as a foliar application in autumn based on 2 litres per hectare against the background of the main fertilizer, calculated for N22Р39К62 4 tons per hectare of grain. Pre-sowing seed treatment with Bioplant Flora at a dose of 0.5 litre per hectare of seeds was used against the background of N22Р39К62 4 tons per hectare of grain. Plant nutrition with Bioplant Flora fertilizer stimulated the growth and development of plants that effectively used nutrients from the soil to increase grain yield. The optimal term for treatment of winter wheat by Bioplant Flora is autumn application for seedlings based on 2 litres per hectare. At the same time, the productivity increased by 0.46-0.53 tons per hectare, depending on the application of Bioplant Flora. Pre-sowing treatment of seeds by Bioplant Flora based on 0.5 litres per ton of seeds against the background of the main fertilizer for 4 tons per hectare of grain, on average for 2010-2012, provided a productivity increase of 0.21 tons per hectare. Against this background, the autumn treatment by Bioplant Flora allowed to increase the productivity to 0.15 tons per hectare, compared to the sample without fertilizers. At the same time, the average for 2010-2012 the profit was obtained - 11981 rubles per hectare, the cost of grain amounted to 3596.0 rubles per ton, the profitability of production - 94.6%.
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Almeida, Rafael Nunes, Diego Rogério Ferraz, Arildo Sebastião Silva, Erivelton Gonçalves Cunha, Josean Castro Vieira, Tássio Da Silva Souza, and Sávio Da Silva Berilli. "UTILIZAÇÃO DE LODO DE CURTUME EM COMPLEMENTAÇÃO AO SUBSTRATO COMERCIAL NA PRODUÇÃO DE MUDAS DE PIMENTA BIQUINHO." Scientia Agraria 18, no. 1 (April 12, 2017): 20. http://dx.doi.org/10.5380/rsa.v18i1.49914.

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A utilização de resíduos industriais, como o lodo de curtume, em substratos para produção de mudas é uma alternativa para redução de custos de produção, e para destinação dos resíduos industriais. O cultivo de pimentas tem ganhado espaço no mercado brasileiro favorecendo principalmente a agricultura familiar, onde um dos desafios é diminuir o custo de produção. O objetivo do trabalho foi avaliar o efeito de diferentes concentrações de lodo de curtume bovino adicionado ao substrato comercial na germinação, desenvolvimento e qualidade de mudas de pimenta biquinho. O experimento foi conduzido em delineamento de blocos casualizados com 7 tratamentos, sendo dois substratos comerciais: Provaso® (TP) e Bioplant® (TL0); e cinco diferentes proporções de lodo desidratado (10, 30, 50, 70 e 90%) misturados ao substrato Bioplant®. Avaliou-se nesse experimento o percentual de germinação, características de desenvolvimento e Índice de Qualidade de mudas de Dickson (IQD). O potencial germinativo foi maior para os tratamentos com adição de lodo de curtume ao substrato comercial Bioplant®. Os melhores resultados para as características de desenvolvimento das mudas ocorreram com a adição de lodo desidratado ao substrato Bioplant® nos intervalos de 30 a 70% de lodo no substrato. O índice de qualidade de mudas foi maior para a mistura de 30% de lodo de curtume desidratado com Bioplant®.
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Miladinov, Zlatica, Igor Balalic, Velimir Radic, Jovan Crnobarac, Milan Jockovic, Goran Jokic, and Vladimir Miklic. "Influence of biostimulators on germination parameters and early growth of sunflower seedling." Journal of Agricultural Sciences, Belgrade 60, no. 1 (2015): 1–9. http://dx.doi.org/10.2298/jas1501001m.

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The aim of this paper was to examine the influence of biostimulators, with or without the use of fungicides, on seed quality parameters and early growth of sunflower seedling. Testing was conducted at the Institute of Field and Vegetable Crops in Novi Sad on cytoplasmic male sterile sunflower line HA-26-PR. The experiment included six treatments: Slavol S (25%), Slavol S (25%) + fungicide (a.m. metalaxyl-M), Bioplant Flora (2%), Bioplant Flora (2%) + fungicide (a.m. metalaxyl-M), Slavol S (25%) + Bioplant Flora (2%), Slavol S (25%) + Bioplant Flora (2%) + fungicide (a. m. metalaxyl-M) and control. Results of the research showed that treating the seed with biostimulators prior to sowing has a significant influence on seed germination parameters and early growth of sunflower seedling. The individual application of fertilizers, with or without the use of fungicide, gave a positive effect, while their combination led to a negative effect. The individual application of fertilizers, with or without combination with fungicide, may increase germination energy by 5%, germinability by 6%, but may also reduce the time of seed germination. Likewise, their use has a positive effect on early growth of sunflower seedling.
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De Oliveira, Jéssica Costa, and Adalberto Brito De Novaes. "MORPHOLOGICAL AND RADICAL PARAMETERS ASSOCIATED WITH THE SEEDLINGS QUALITY OF Anadenanthera peregrina (L.) Speg. IN DIFFERENT SUBSTRATES." FLORESTA 50, no. 3 (July 10, 2020): 1467. http://dx.doi.org/10.5380/rf.v50i3.61577.

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Morphological and Radical Parameters Associated with the Seedlings Quality of Anadenanthera peregrina (L.) Speg.in Different Substrates. In this research, we evaluated the effects of different substrate formulations on the quality of angico-vermelho seedlings (Anadenanthera peregrina (L.) Speg). The tested substrates corresponded to eight different formulations: Bioplant®, Carolina Soil, Coffee husk, Coconut fiber and Vermiculite. In the nursery phase, the following were evaluated: height of the aerial part (H); neck diameter (D); fresh and dry biomass of the aerial parts and radical system; dickson quality index (IQD); number of roots regenerated in tubes; and length of roots regenerated in boxes. In the field phase, the following were evaluated: survival; crown diameter; number of lateral roots; and length of the pivoting root. To produce seedlings, a completely randomized design with eight treatments and four replications was used, and for the evaluation of the root regeneration potential (P.R.R.), six replications were used. In the field, the experimental arrangement consisted of subdivided plots, arranged in randomized blocks with four replications. Seedlings with higher averages for most of the evaluated characteristics were produced in the formulation of substrate containing 50% Bioplant® + 30% coffee husk + 20% coconut fiber. The values related to the IQD were positively related to the substrate formulations containing Bioplant®, coffee husk and coconut fiber, which were also positively associated with P.R.R. The substrate formulations containing Bioplant®, coffee husk and coconut fiber resulted in seedlings with a higher P.R.R., evaluated in boxes. Substrates containing Bioplant®, Carolina Soil, coffee husk and coconut fiberfavored the production of seedlings with a better radical system and, consequently, better in the field.
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Dutra, Tiago Reis, Marília Dutra Massad, Mateus Felipe Quintino Sarmento, and Jéssica Costa de Oliveira. "Substratos alternativos e métodos de quebra de dormência para produção de mudas de canafístula." Revista Ceres 60, no. 1 (February 2013): 72–78. http://dx.doi.org/10.1590/s0034-737x2013000100011.

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O objetivo deste estudo foi avaliar o efeito de diferentes métodos de quebra de dormência e o uso do bagaço de cana para composição de substratos alternativos, constituídos a partir da mistura de um substrato comercial, na produção de mudas de canafístula (Peltophorum dubium (Sprengel) Taubert). O experimento foi conduzido em delineamento de blocos casualizados, no esquema fatorial 5 x 4, sendo cinco substratos, Bioplant®; 75% Bioplant® + 25% Bagaço de cana (75B+25BC); 50% Bioplant® + 50% Bagaço de cana (50B+50BC); 25% Bioplant® + 75% Bagaço de cana (25B+75BC); Bagaço de cana (100%), e quatro métodos de quebra de dormência (testemunha, escarificação com o uso de lixa, imersão em água quente e escarificação com ácido sulfúrico) com três repetições. Foram avaliados o índice de velocidade de emergência (IVE); o tempo médio de emergência (TME); a percentagem de germinação; a altura da parte aérea; o diâmetro do coleto; a massa seca da parte aérea; a massa seca de raiz; a massa seca total; a relação altura da parte aérea/diâmetro do coleto; a relação altura da parte aérea/massa seca da parte aérea; o índice de qualidade de Dickson; a taxa de crescimento absoluto e a taxa de crescimento relativo. Os tratamentos de imersão em água quente e de escarificação com ácido sulfúrico foram responsáveis pelos maiores percentuais de emergência e IVE, além dos de menor TME. O uso do bagaço de cana mostrou-se tecnicamente viável na composição dos substratos 75B+25BC e 50B+50BC, que, juntamente ao Bioplant®, proporcionaram as maiores taxas de crescimento e os maiores índices de qualidade das mudas de canafístula.
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Jeromini, Tatiane Sanches, Ricardo Fachinelli, Givanildo Zildo da Silva, Suzana Targanski Sajovic Pereira, and Silvana De Paula Quintão Scalon. "Emergência de plântulas e crescimento inicial de copaíba sob diferentes substratos." Pesquisa Florestal Brasileira 37, no. 90 (June 30, 2017): 219. http://dx.doi.org/10.4336/2017.pfb.37.90.1189.

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O objetivo do trabalho foi avaliar uma emergência e crescimento inicial de mudas de copaíba ( Copaifera langsdorffi Desf.) Sob diferentes substratos. A semeadura foi realizada nos substratos: solo, Bioplant®, solo + areia, solo + Bioplant® e solo + areia + cama-de-frango. Foram avaliadas, velocidade de emergência e crescimento de plântulas. Para uma emergência de plântulas de copaíba são indicados todos os substratos, exceto mistura solo + areia + cama-de-frango. Para o crescimento inicial, indicado pelo índice de qualidade de Dickson, o melhor substrato foi solo solo.
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Cvijanović, Gorica, Ninoslav Čolić, Nenad Đurić, Gordana Dozet, Abduladim Eltreki, Marija Cvijanović, and Milena Žuža. "Effect of biostimulants on soybean seedlings." Acta agriculturae Serbica 25, no. 50 (2020): 99–104. http://dx.doi.org/10.5937/aaser2050099c.

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The aim of this study was to analyze the effect of biostimulants on the morphological characteristics of soybean seedlings. The testing was conducted in the laboratory of the Faculty of Biofarming in Bačka Topola. The experimental material included three soybean varieties ('Galina', 'Sava' and 'Rubin') selected at the Institute of Field and Vegetable Crops in Novi Sad. The study lasted for two years, 2015-2016, and identical biostimulant treatments were applied in both years. In order to determine the effect of biostimulants on soybean seedling root, hypocotyl and weight, the following commercial biostimulants were applied: EM Aktiv, Terra Green Hobby, Slavol and Bioplant Flora. In addition to the single application of biostimulants, two combinations of Slavol + Bioplant Flora and Slavol + Bioplant Flora + Epin Extra + Slavol S were used as treatments. EM Aktiv showed the greatest effect on root growth. The root was on average 12% longer than the control. Slavol S had the greatest influence on seedling hypocotyl and weight. The increase was 8.24% and 5.15%, respectively, compared with the control.
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Lima, Isabela Machado de Oliveira, Joaquim Souto Silva Júnior, Edilson Costa, Eliana Duarte Cardoso, Flávio Ferreira da Silva Binotti, and Marçal Henrique Amici Jorge. "DIFERENTES SUBSTRATOS E AMBIENTES PROTEGIDOS PARA O CRESCIMENTO DE MUDAS DE MARACUJAZEIRO AMARELO DOCE." JOURNAL OF NEOTROPICAL AGRICULTURE 3, no. 4 (October 17, 2016): 39–47. http://dx.doi.org/10.32404/rean.v3i4.1240.

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A muda de alta qualidade, com vigor superior e equilíbrio de crescimento em altura e diâmetro, possui condições de melhor pegamento e sobrevivência no local definitivo de plantio. Objetivou-se estudar vários substratos contendo diferentes mistura de esterco bovino, solo de barranco, Bioplant®, vermiculita super fina e areia fina em ambientes telados na formação de mudas de maracujá. Foram utilizados o (A1) telado agrícola (dimensões: 8,0 m x 18,0 m x 3,5 m), fechamento em 45º de inclinação, com tela preta de monofilamento em toda sua extensão, malha para 50% de sombreamento (Sombrite®) e (A2) telado agrícola (dimensões: 8,0 m x 18,0 m x 4,0 m), com tela aluminizada termorrefletora na cobertura a 3,30 m, malha para 50% de sombreamento (Aluminet®), fechamentos laterais e frontais em 90º de inclinação com tela de monofilamento preta, malha para 50% de sombreamento. No interior dos ambientes protegidos, as mudas foram formadas em 13 substratos (S) oriundos das combinações (%) de esterco bovino (E), solo de barranco (S), Bioplant® (B), vermiculita super fina (F) e areia (A). Foram avaliadas a emergência, a altura, o diâmetro do colo e a relação entre a altura e o diâmetro. As melhores mudas de maracujazeiro amarelo doce foram formadas em substratos com maiores quantidade de esterco e/ou vermiculita, com menores quantidades de areia e Bioplant®. Substratos com elevadas quantidades de Bioplant® ou areia não são indicados à formação de mudas de maracujazeiro. O telado aluminizado propiciou condições para obtenção de mudas de maior qualidade, com maior diâmetro em alguns substratos, e menor relação altura/diâmetro.
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Moatti, J. P., N. Dousset, N. Moattit, M. Degre, B. Eche, G. Gasset, and R. Tixador. "Esa's biopan 1 — “Vitamin” experiment preliminary results." Acta Astronautica 36, no. 8-12 (October 1995): 527–33. http://dx.doi.org/10.1016/0094-5765(95)00138-7.

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Demets, R., W. Schulte, and P. Baglioni. "The past, present and future of Biopan." Advances in Space Research 36, no. 2 (January 2005): 311–16. http://dx.doi.org/10.1016/j.asr.2005.07.005.

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Chaerul, Mochammad. "Performance of Bioplant for Municipal Solid Waste at Jatinangor Campus of Institut Teknologi Bandung." E3S Web of Conferences 148 (2020): 02009. http://dx.doi.org/10.1051/e3sconf/202014802009.

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Although Law No. 18 year of 2018 has mandated recycling to be prioritized in solid waste management, the municipality intend to collect and to dump the waste directly to landfill. By cooperating with National Gas Company (PT. PGN), bioplant was constructed at area of 200 m2 and operated at Jatinangor Campus of Institut Teknologi Bandung to treat organic waste generated from the campus and traditional market near the campus. The study aims to evaluate the performance of the bioplant in term of biogas generation and composition during the pilot project. Main treatment at the bioplant is anaerobic digestion (AD) using two units with single-stage and wet fermentation. Organic waste as feeding had 62%, 61% of water and volatile contents, respectively. Carbon organic and total Nitrogen contributed 8.19% and 0.22%, respectively. 200 kg of the organic waste daily was mixed with ratio of 1:1 with water. Total volume biogas generated daily from 2 units of AD was 2.1 m3 with composition of 45%, 15%, and 10% of CH4, CO2 and O2, respectively. Due to the waste reduction at source, various potential negative impacts due to open dumping commonly operated at landfill site in Indonesia could be avoided.
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Butti, Matias D., Hernan Chanfreau, Diego Martinez, Diego García, Ezequiel Lacunza, and Martin C. Abba. "BioPlat: a software for human cancer biomarker discovery." Bioinformatics 30, no. 12 (February 25, 2014): 1782–84. http://dx.doi.org/10.1093/bioinformatics/btu111.

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Agapito, Giuseppe, Chiara Pastrello, Pietro Hiram Guzzi, Igor Jurisica, and Mario Cannataro. "BioPAX-Parser: parsing and enrichment analysis of BioPAX pathways." Bioinformatics 36, no. 15 (May 21, 2020): 4377–78. http://dx.doi.org/10.1093/bioinformatics/btaa529.

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Abstract Summary Biological pathways are fundamental for learning about healthy and disease states. Many existing formats support automatic software analysis of biological pathways, e.g. BioPAX (Biological Pathway Exchange). Although some algorithms are available as web application or stand-alone tools, no general graphical application for the parsing of BioPAX pathway data exists. Also, very few tools can perform pathway enrichment analysis (PEA) using pathway encoded in the BioPAX format. To fill this gap, we introduce BiP (BioPAX-Parser), an automatic and graphical software tool aimed at performing the parsing and accessing of BioPAX pathway data, along with PEA by using information coming from pathways encoded in BioPAX. Availability and implementation BiP is freely available for academic and non-profit organizations at https://gitlab.com/giuseppeagapito/bip under the LGPL 2.1, the GNU Lesser General Public License. Supplementary information Supplementary data are available at Bioinformatics online.
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Binnicker, Matthew J., Deborah J. Jespersen, and Leonard O. Rollins. "Evaluation of the Bio-Rad BioPlex Measles, Mumps, Rubella, and Varicella-Zoster Virus IgG Multiplex Bead Immunoassay." Clinical and Vaccine Immunology 18, no. 9 (July 27, 2011): 1524–26. http://dx.doi.org/10.1128/cvi.05207-11.

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ABSTRACTThe goal of this study was to compare the BioPlex 2200 measles, mumps, rubella, and varicella-zoster virus (MMRV) IgG multiplex assays (Bio-Rad Laboratories, Hercules, CA) to routine testing by enzyme immunoassay (EIA). Serum specimens (n= 500) submitted to our reference laboratory for routine MMRV IgG testing by EIA were also tested by the BioPlex assays. Following testing, the BioPlex measles, mumps, rubella, and varicella-zoster virus assays demonstrated agreements of 91.6% (95% confidence interval [CI], 88.8% to 93.7%), 94.2% (95% CI, 91.7% to 95.7%), 94.4% (95% CI, 92.0% to 96.1%), and 91.8% (95% CI, 89.0% to 93.9%), respectively, compared to the results of EIA. Timing studies showed that the BioPlex MMRV assay could provide complete analysis of 100 serum specimens in 1.7 h, compared to 5.5 h by EIA. These data indicate that the BioPlex MMRV IgG assays exhibit comparable performance (93% overall agreement [1,860/2,000 results]; κ = 0.67) to routine testing by EIA. The BioPlex assays allow for the simultaneous detection of all four analytes, thereby eliminating potential aliquot errors and reducing turnaround time.
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Pérez, Dolores, Boris Gilburd, Óscar Cabrera-Marante, Jose A. Martínez-Flores, Manuel Serrano, Laura Naranjo, Daniel Pleguezuelo, et al. "Predictive autoimmunity using autoantibodies: screening for anti-nuclear antibodies." Clinical Chemistry and Laboratory Medicine (CCLM) 56, no. 10 (September 25, 2018): 1771–77. http://dx.doi.org/10.1515/cclm-2017-0241.

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Abstract Background: Early detection of antinuclear antibodies (ANA) in asymptomatic subjects is useful to predict autoimmune diseases years before diagnosis. ANA have been determined by indirect immunofluorescence (IIF) using human epithelial type 2 (HEp-2) cells, which is considered the gold standard technique. Multiplex technology (BioPlex ANA Screen) has been introduced for ANA evaluation in recent years. Nevertheless, concordance between BioPlex and IIF is low and there is no harmonization between both methods for detection of autoantibodies. This study has aimed to clarify the clinical significance of autoantibodies detected by BioPlex ANA Screen in subjects with undiagnosed clinical suspicion of autoimmune disease and to determine the predictive value of autoantibodies detected by BioPlex ANA Screen. Methods: A 3-year follow-up study was performed of 411 subjects without a clear diagnosis of autoimmune diseases in whom autoantibodies were detected by BioPlex ANA Screen that were negative by IIF on HEp-2 cells. Results: At 3 years of follow-up, 312 (76%) subjects were positive for autoantibodies by IIF and 99 subjects continued to be negative. A diagnosis of autoimmune disease was found in most of the subjects (87%). Conclusions: BioPlex ANA Screen has greater sensitivity than IIF on HEp-2 cells for autoantibodies detection. Early detection of these antibodies by BioPlex can predict possible development of autoimmune diseases.
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Sammons, Jonathan D., and Daniel K. Struve. "Effect of Bioplex™ on Transplant Success and Recovery of Summer-dug Goldenraintree." Journal of Environmental Horticulture 23, no. 2 (June 1, 2005): 59–62. http://dx.doi.org/10.24266/0738-2898-23.2.59.

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Abstract Biostimulants are intended to reduce stress associated with non-dormant (summer-dug) harvest of field-grown nursery stock; however, the effectiveness of biostimulant treatment is uncertain. We tested the effects of three application methods of Bioplex™ (a biostimulant) on transpiration rates, transplant survival, and recovery of field-grown goldenraintree (Koelrueteria paniculata Laxm.), which is considered difficult to transplant and is rarely summer dug. Bioplex™ was applied as a foliar spray, soil drench, or a combination of foliar spray and soil drench. Bioplex™ reduced transpiration rates of trees by 12% compared to untreated control trees. Root loss associated with digging reduced transpiration rates and had a greater effect on transpiration than any Bioplex™ treatment. Survival 12 months after transplanting was 100%. Bioplex™ treatments applied before digging had no effect on growth after transplanting. Transplanted trees had reduced caliper growth and shoot extension the season after transplant compared to non-dug controls. Although Bioplex™ reduced transpiration rates for three to five days after application in non-dug trees, there were no long-term benefits to survival or re-growth to summer transplanted trees.
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STOBIE, CHERYL. "‘Biopia’ in biography." Scrutiny2 9, no. 2 (January 2004): 35–52. http://dx.doi.org/10.1080/18125441.2004.9684189.

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Rodchenkov, I., E. Demir, C. Sander, and G. D. Bader. "The BioPAX Validator." Bioinformatics 29, no. 20 (August 5, 2013): 2659–60. http://dx.doi.org/10.1093/bioinformatics/btt452.

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Flora, S. V., E. V. Chentsova, and E. N. Iomdina. "Crosslinking of the Bioplast plastic material as a means of increasing its biomechanical stability and the effectiveness of keratoplasty for corneal ulcers." Russian Ophthalmological Journal 14, no. 2 (June 20, 2021): 36–41. http://dx.doi.org/10.21516/2072-0076-2021-14-2-36-41.

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Purpose:to assess the effect of ultraviolet (UVA) crosslinking of Bioplast plastic material (silicon-dried human cornea) on its biomechanical stability and effectiveness for keratoplasty in corneal ulcers. Material and methods. To determine the maximum rehydration time, 12 Bioplast samples were incubated in physiological solution (NaCl 0.9 %) for 12 hours and monitored for thickness using optical coherence tomography (OCT Spectralis, Heidelberg Engineering). After the epithelium was scarified rehydrated corneas were treated withof 0.1% riboflavin solution for 30 minutes, and after it 8 rehydrated samples (experimental group) were treated with UVA for 30minutes (wavelength 370 nm, power 3 mW/cm2) using a UV-X device system (IROC AG, Switzerland). In the control group (4 rehydrated samples), no UVA crosslinking was performed. The elastic-strength indicators of the experimental and control group samples were assessed by applying uniaxial tension using a deformation machine (Autograph AGS-H, Japan). To examine the patients (presented in clinical examples)before and after keratoplasty with Bioplast subjected to UVA crosslinking, a set of standard ophthalmic tests was used. Results.Rehydration of Bioplast was the most intensive in the first three hours of incubation, during which time tissue thickness increased uniformly by 50–55 μm/hour to 700–710 μm, while further incubation showed no significant additional effect on the fluid content in the cornea. After UVA crosslinking, the strength indices and the elastic modulus of the rehydrated Bioplast samples significantly increased in comparison with the control group. Clinical examples demonstrate the effectiveness of modified Bioplast for organ-preserving penetrating keratoplasty in case of perforatedcorneal ulcer. Conclusion. UVA crosslinking of rehydrated Bioplast increases its biomechanical stability. The data of laboratory studies and the first clinical tests demonstrate that modified Bioplast may be an effective replacement of native donor cornea in urgent keratoplasty of perforated ulcers.
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Fernando Vieira da Silva, Luis, Edmar Isaías de Melo, and Pablo Arthur Silva Gonçalves. "BIOCHAR DE SERRAGEM DE EUCALIPTO COMO CONDICIONADOR DE SUBSTRATOS PARA PRODUÇÃO DE MUDAS DE ALFACE." AGRI-ENVIRONMENTAL SCIENCES 5 (November 6, 2019): 0. http://dx.doi.org/10.36725/agries.v5i0.1614.

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O sucesso na produção de alface (Lactuca sativa L.) começa pela obtenção de mudas com boa qualidade sendo necessário a utilização de substratos que fornecem condições químicas e físicas favoráveis ao desenvolvimento da planta. O reaproveitamento de resíduos na produção de substratos pode ser uma proposta interessante para a produção de mudas. O trabalho objetivou avaliar a influência de biochar produzido a partir da serragem de eucalipto, no desenvolvimento de mudas de alface. O experimento foi conduzido em casa de vegetação com delineamento experimental inteiramente casualizado, com sete tratamentos e três repetições. Utilizou-se a cultivar Grandes Lagos e sete substratos sendo um comercial, Bioplant® (BPL) e seis formados a partir do substrato comercial, Bioplant® + biochar (BC 5, BC 10, BC 15, BC 25, BC 50 e BC 75% m/m). Foram avaliadas as variáveis percentagem de emergência (E), índice de velocidade de emergência (IVE), comprimento da parte aérea (CPA) e sistema radicular (CSR), massa fresca da parte aérea (MFPA) e do sistema radicular (MFSR) e massa seca da parte aérea (MSPA) e do sistema radicular (MSSR), 25 dias após a semeadura. A adição de biochar, ao substrato comercial, Bioplant®, na concentração de 5% em massa, apresentou viabilidade para ser utilizado como condicionador de substrato no desenvolvimento de mudas de alface da cultivar Grandes Lagos.
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Da Silva Berilli, Sávio, Carla Basílio Monteiro, Ana Terra Bravim dos Santos, Fernanda Emanuele Silva Costa, Ringo Souza Batista, Hélio Orlando Menegueli, Francisco José Valim Olmo, and Vinicius Rodrigues Ferreira. "EFEITO DO LODO DE CURTUME ADICIONADO EM SUBSTRATO COMERCIAL PARA PRODUÇÃO DE MUDAS DE PIMENTA MALAGUETA." Revista Ifes Ciência 6, no. 2 (August 28, 2020): 149–62. http://dx.doi.org/10.36524/ric.v6i2.560.

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O aproveitamento de resíduos agroindustriais é pouco recorrente, embora seja viável economicamente e benéfico ao meio ambiente. Por sua vez, tais materiais apresentam elevadas porções de matéria orgânica e nutrientes, indicando alto potencial em utilização agrícola. Portanto, objetivou-se avaliar o desenvolvimento e qualidade de mudas de pimenta malagueta, cultivadas em seis diferentes substratos, contendo proporções de 0 a 90% de lodo de curtume em substrato comercial Bioplant®, além do substrato testemunha, o Provaso®. Aos 21 dias após a semeadura foi contabilizada a emergência das plântulas e, aos 42 dias, as características de desenvolvimento e qualidade de mudas. Para a maioria das variáveis analisadas, o lodo de curtume, nos substratos, promoveu um bom desenvolvimento das plântulas de pimenteira, apresentando desempenho semelhante ao Provaso®. Todavia, o aumento das porções de lodo, aplicadas acima de um nível ótimo, tem efeito negativo, sendo observado declínio nos parâmetros de crescimento e qualidade das plântulas. Além disso, observou-se que o Bioplant® necessita de suplementação mineral para atender as necessidades iniciais das plântulas, sendo essa basicamente sustentada pelo lodo de curtume adicionado a mistura do substrato. Por fim, o aproveitamento deste resíduo, como complemento ao substrato comercial Bioplant®, para produção de mudas de pimenta, mostrou-se viável agronomicamente, promovendo uma boa qualidade e satisfatório desenvolvimento das mudas.
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Arbefeville, Sophie, Maureen Lynch, and Patricia Ferrieri. "Evaluation of a Multiplex Fully Automated Treponemal and Nontreponemal (Rapid Plasma Reagin) Assay." American Journal of Clinical Pathology 152, no. 2 (May 29, 2019): 230–36. http://dx.doi.org/10.1093/ajcp/aqz034.

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ABSTRACTObjectivesIn June 2017, Bio-Rad Laboratories received US Food and Drug Administration clearance for its BioPlex 2200 Syphilis Total & RPR (rapid plasma reagin) assay. It is the first fully automated treponemal/nontreponemal multiplex flow immunoassay, simultaneously detecting Treponema pallidum and reagin antibodies and an RPR titer. We compared the performance of the BioPlex Syphilis Total & RPR assay with the LIAISON Treponema Assay and the manual BD Macro-Vue RPR 18-mm Circle Test.MethodsIn total, 314 serum specimens were tested for treponemal immunoglobulin G/immunoglobulin M and RPR with the LIAISON Treponema Assay, the BioPlex 2200 Syphilis Total & RPR assay, and the manual BD Macro-Vue RPR card test. All discordant results were further tested with the T pallidum particle agglutination assay from Fujirebio Diagnostics.ResultsThe overall percent agreement for the BioPlex assay for treponemal antibodies with the LIAISON Treponema Assay was 96.1%. Sensitivity and specificity for the BioPlex RPR assay were 90.5% and 97.2%, respectively (the manual RPR assay was considered the gold standard).ConclusionsThe BioPlex 2200 Syphilis Total & RPR assay performance was comparable to the LIAISON Treponema Assay and the manual RPR test. Compared with the manual RPR, the automation of RPR testing offered labor savings, objective result reporting, and improved workflow.
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Dornelles, Paulo, Fabiano Guimarães Silva, Clenilso Sehnen Mota, and João das Graças Santana. "Production and quality of Anacardium othonianum Rizz. seedlings grown in different substrates." Revista Brasileira de Fruticultura 36, no. 2 (June 2014): 479–86. http://dx.doi.org/10.1590/0100-2945-144/13.

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This study aimed to evaluate the effect of substrate on growth, emergence, nutrition, and quality of Anacardium othonianum Rizz. (cerrado cashew tree) seedlings. The experiment was conducted in a greenhouse at the Plant Tissue Culture Laboratory on the Rio Verde campus. The following substrates were used: 1) Bioplant®, 2) Mecplant® (MP) + carbonized rice husk (CRH) (7:3), 3) fine-grained vermiculite (FGV), 4) FGV+CRH (3:1), 5) FGV+CRH (1:1), 6) FGV+CRH (1:3), and 7) sugarcane bagasse (SB) + sugarcane mill filter cake (FC) (3:2). Emerged seedlings were counted at 2-day intervals for 38 days following emergence of the first seedling. At 39, 64, and 89 days after seeding (DAS), the following variables were measured: stem length (SL), stem diameter (SD), and number of leaves (NL). Accumulated dry weight, quality indices, and leaf macro- and micronutrient levels were determined at 89 DAS. Plants grown in the FGV and FGV+CFH (1:3) substrates had shorter stem lengths than the plants grown in other substrates. Increases in seedling growth were smaller between 64 and 89 DAS compared to the initial period of the experiment. The highest leaf N concentrations were found in the SB+FC substrate treatment group; P and K concentrations were higher for the MP+CRH (7:3), SB+FC, and Bioplant® treatments; and Ca levels were higher for the SB+FC and MP+CRH (7:3) substrate treatments. The MP+CRH (7:3) substrate treatment group had the highest leaf B and Mn micronutrient concentrations, and plants from the Bioplant® substrate group had the highest leaf B micronutrient content. Mg, S, Cu, Zn, and Fe concentrations did not differ among the different substrates. The plant traits that differed most among the treatments included stem length for the FGV and FGV+CRH (1:3) substrate groups and leaf nutrient concentrations, which were higher for the SB+FC group followed by the MP+CRH and Bioplant® treatments.
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Silva, Luiz Francisco Tavares da, Thaynnara Paula dos Santos Lira, Victória Polyana Azevedo Araújo, Lívia Gibrinde Barbosa, Letícia Paula Barros Lima, and Rubens Pessoa de Barros. "™Índice de germinação (IG) e índice de velocidade de germinação (IVG) do tomate cereja (Solanum lycopersicum var. cerasiforme, solanaceae) cultivadas em vasos sob diferentes substratos." Diversitas Journal 5, no. 1 (January 13, 2020): 50–56. http://dx.doi.org/10.17648/diversitas-journal-v5i1-896.

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RESUMO: Objetivou-se com este estudo avaliar os índices de germinação (IG) e de velocidade de germinação (IVG) do Tomate Cereja (Solanum lycopersicum var. cerasiforme) em diferentes substratos em ambiente protegido. A área da pesquisa foi na casa de vegetação da Universidade Estadual de Alagoas, Campus I, em Arapiraca-AL durante o período de Março a abril/2019. Os substratos utilizados foram areia fina, areia lavada, solo de área agrícola e Bioplant®. A semeadura ocorreu em vasos em material plástico, e foram realizadas sendo 5 repetições em cada tipo de solo, totalizando 20 amostras. Foram analisados o IG e o IGV de cada amostra no período de 30 dias. Foi possível identificar que o tomate cereja apresentou melhor IG e IGV nos substratos Areia Fina e Bioplant. PALAVRAS-CHAVE: desenvolvimento, sementes, solo.
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31

Lessire, Sarah, Jonathan Douxfils, Lionel Pochet, Anne-Sophie Dincq, Anne-Sophie Larock, Maximilien Gourdin, Jean-Michel Dogné, Bernard Chatelain, and François Mullier. "Estimation of Rivaroxaban Plasma Concentrations in the Perioperative Setting in Patients With or Without Heparin Bridging." Clinical and Applied Thrombosis/Hemostasis 24, no. 1 (November 3, 2016): 129–38. http://dx.doi.org/10.1177/1076029616675968.

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Introduction: Estimation of residual rivaroxaban plasma concentrations may be requested before invasive procedures and some patients at high thromboembolic risk will have a bridging therapy with heparins when rivaroxaban is interrupted. Objective: The objective of this study was to assess the performance of the STA-Liquid Anti-Xa assay (STA LAX) and the low and normal procedures of the Biophen Direct Factor Xa Inhibitors (DiXaI) assay, in patients with and without bridging with low-molecular-weight heparins (LMWHs). Materials and Methods: Seventy-nine blood samples were collected from 77 patients on rivaroxaban at CTROUGH or before an invasive procedure. Rivaroxaban plasma concentrations were estimated using Biophen DiXaI, Biophen DiXaI LOW, and STA LAX and compared to liquid chromatography coupled with mass spectrometry (LC-MS/MS) measurements. Stratifications were performed according to heparin bridging. Results: The Biophen DiXaI LOW and STA LAX showed better correlation with LC-MS/MS measurements than Biophen DiXaI in patients not bridged with LMWH (R: 0.97, 0.96, and 0.91, respectively). However, the performance of Biophen DiXaI LOW and STA LAX decreased when residual LMWH activity was present (R: 0.18 and 0.19 respectively) demonstrating that these tests are not specific to rivaroxaban. Conclusion: In patients not bridged with LMWH, we suggest to use the Biophen DiXaI LOW and STA LAX for the estimation of rivaroxaban concentrations <50 ng/mL. These results should be confirmed on a larger cohort of patients. Patients bridged with LMWH have inaccurate estimates of low levels of rivaroxaban and the 3 assays studied should not be used to estimate if it is safe to perform a procedure.
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Petrovič, Vladimír, Ján Kushev, Lode Nollet, and Gabriel Kováč. "Effect of dietary supplementation of trace elements on blood chemistry and selected immunological indices depending on the age of broiler chickens." Acta Veterinaria Brno 80, no. 1 (2011): 57–64. http://dx.doi.org/10.2754/avb201180010057.

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This study was performed to determine the effects of dietary supplementation of Cu, Zn, Fe, Mn and Se on some haematological, immunological and enzymatic indices in blood of broilers at the age of 21, 35 and 42 days. The basal diets (BDs) for Groups 1 and 2 were supplemented with the equivalent amounts of trace elements in an inorganic form (Cu sulphate 5 mg·kg-1 DM, Fe sulphate, Zn oxide, Mn oxide 50 mg·kg-1 DM), however sodium selenite or selenized yeast (Sel-Plex) were given at a dose of Se 0.3 mg·kg-1 DM, respectively. Groups 3 and 4 received the same BD´s but with the substantially reduced amount of supplemented nutrients in the organic “proteinated” form (Bioplex Cu 2.5 mg·kg-1 DM, Bioplex Fe, Bioplex Zn, Bioplex Mn 10 mg·kg-1 DM), except for selenium that was given at a dose of Se 0.3 mg·kg-1 DM as sodium selenite or Sel-Plex, respectively. The supplementation of restricted doses of trace elements in the organic forms into the diet did not affect studied haematological, immunological, enzymatic indices. At the age of 21 days, the activity of GSH-Px was significantly higher in the groups supplemented with sodium selenite later no differences due to the administered form of Se were found. This indicates that the GSH-Px activity reached a plateau in all groups of broilers at the 21, 35 and 42 day, except for GSH-Px activity in the groups of birds supplemented with selenized yeast on day 21. In Addition, significant age dependent changes were observed in blood chemistry, but the different treatments did not influence these changes. Base on our results, the doses of organic forms of trace elements supplemented into the diets for poultry can be restricted to 50% (Bioplex Cu) and 20% (Bioplex Fe, Bioplex Zn and Bioplex Mn) without influencing the blood chemistry of broilers.
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Сабирова, Razina Sabirova, Шакиров, and Rafil Shakirov. "Productivity of Кazanskaya 560 winter wheat variety, depending on fertilizers and soil nutrient." Vestnik of Kazan State Agrarian University 8, no. 4 (January 13, 2014): 134–37. http://dx.doi.org/10.12737/2439.

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The article discusses the influence of the main fertilizer in combination with additional fertilizing on the feeding regime of soil and productivity of “Kazanskaya 560” winter wheat variety. The provision of soil with available nutrients is achieved through the rational use of fertilizers in crop rotations. In 2010-2012 an additional fertilizing by Humate “Bioplant Flora” at a rate of 2 litre per hectare in autumn increased yield to 4.6-5.5 centner per hectare, depending on the background of the basic fertilizer. Additional spring fertilizing in early plant growth by Bioplant Flora on the background of autumn feeding practically does not increase the yield of winter wheat. Fertilizing with ammonium nitrate at a rate of 1.5 center in share weight per hectare increased the average yield to 2.1-2.4 tons per hectare for the period 2010-2012, depending on the background of the main treatment. In favorable 2011 it increased the yield to 1.5-3.5 centner per hectare. Adding NPK at sowing in rows at 1 centner per hectare in share weight increases yields to 3.5 centner per hectare without feeding, to 7.5 centner per hectare with the autumn fertilizing by Bioplant Flora, to 5.6 centner per hectare with the spring fertilizing with ammonium nitrate, comparing with the background without fertilizers. Thus, the differential application of the basic fertilizer, combined with fertilizing, improves the soil nutrient status and facilitates the formation of a predetermined amount of yield.
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Gaud, Caroline, Bebiana C. Sousa, An Nguyen, Maria Fedorova, Zhixu Ni, Valerie B. O’Donnell, Michael J. O. Wakelam, Simon Andrews, and Andrea F. Lopez-Clavijo. "BioPAN: a web-based tool to explore mammalian lipidome metabolic pathways on LIPID MAPS." F1000Research 10 (January 6, 2021): 4. http://dx.doi.org/10.12688/f1000research.28022.1.

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Lipidomics increasingly describes the quantitation using mass spectrometry of all lipids present in a biological sample. As the power of lipidomics protocols increase, thousands of lipid molecular species from multiple categories can now be profiled in a single experiment. Observed changes due to biological differences often encompass large numbers of structurally-related lipids, with these being regulated by enzymes from well-known metabolic pathways. As lipidomics datasets increase in complexity, the interpretation of their results becomes more challenging. BioPAN addresses this by enabling the researcher to visualise quantitative lipidomics data in the context of known biosynthetic pathways. BioPAN provides a list of genes, which could be involved in the activation or suppression of enzymes catalysing lipid metabolism in mammalian tissues.
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Gaud, Caroline, Bebiana C. Sousa, An Nguyen, Maria Fedorova, Zhixu Ni, Valerie B. O’Donnell, Michael J. O. Wakelam, Simon Andrews, and Andrea F. Lopez-Clavijo. "BioPAN: a web-based tool to explore mammalian lipidome metabolic pathways on LIPID MAPS." F1000Research 10 (June 9, 2021): 4. http://dx.doi.org/10.12688/f1000research.28022.2.

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Lipidomics increasingly describes the quantification using mass spectrometry of all lipids present in a biological sample. As the power of lipidomics protocols increase, thousands of lipid molecular species from multiple categories can now be profiled in a single experiment. Observed changes due to biological differences often encompass large numbers of structurally-related lipids, with these being regulated by enzymes from well-known metabolic pathways. As lipidomics datasets increase in complexity, the interpretation of their results becomes more challenging. BioPAN addresses this by enabling the researcher to visualise quantitative lipidomics data in the context of known biosynthetic pathways. BioPAN provides a list of genes, which could be involved in the activation or suppression of enzymes catalysing lipid metabolism in mammalian tissues.
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36

Binnicker, M. J., D. J. Jespersen, and J. A. Harring. "Multiplex Detection of IgM and IgG Class Antibodies to Toxoplasma gondii, Rubella Virus, and Cytomegalovirus Using a Novel Multiplex Flow Immunoassay." Clinical and Vaccine Immunology 17, no. 11 (September 22, 2010): 1734–38. http://dx.doi.org/10.1128/cvi.00332-10.

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ABSTRACT The goal of this study was to evaluate the BioPlex 2200 Toxoplasma, rubella, and cytomegalovirus (CMV) (ToRC) IgG and IgM multiplex immunoassays (Bio-Rad Laboratories, Hercules, CA) and compare the results to those of conventional testing by enzyme immunoassay (EIA) and enzyme-linked fluorescent assay (ELFA). Serum specimens (n = 600) submitted for routine ToRC IgG and IgM testing by EIA (SeraQuest, Doral, FL; Diamedix, Miami, FL) or ELFA (Vidas; bioMérieux, Durham, NC) were also tested by the BioPlex ToRC multiplex immunoassays. Samples showing discordant results were retested by both methods, with further discrepancies being arbitrated by a third assay. Following repeat testing, the BioPlex Toxoplasma, rubella, and CMV IgG assays demonstrated agreements of 98.7 (592/600 specimens), 93.3 (560/600 specimens), and 98.3% (590/600 specimens), respectively, while the ToRC IgM assays yielded agreements of 91.2 (547/600 specimens), 87.3 (524/600 specimens), and 95.2% (571/600 specimens), respectively. The BioPlex ToRC IgG assays provided results comparable to EIA/ELFA results, with kappa coefficients showing near-perfect agreement for the Toxoplasma (κ = 0.94) and CMV (κ = 0.97) IgG assays and substantial agreement for the rubella IgG assay (κ = 0.66). The BioPlex ToRC IgM assays showed lower specificity with only slight agreement for Toxoplasma IgM (κ = 0.07), poor agreement for rubella IgM (κ = −0.03), and moderate agreement for CMV IgM (κ = 0.55). Both the BioPlex IgG and IgM assays reduced turnaround time (1.7 h versus 5.5 h by EIA/ELFA for 100 specimens) and eliminated the necessity to manually pipette or aliquot specimens prior to testing.
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Melo, Edmar Isaias de, and Luis Fernando Vieira da Silva. "Biocarvão como condicionador de substrato para produção de mudas de alface." Agropecuária Técnica 39, no. 2 (October 25, 2018): 107. http://dx.doi.org/10.25066/agrotec.v39i2.38692.

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A utilização de biocarvão na produção de substratos pode ser uma estratégia agronômica interessante para produção de mudas. Com o objetivo de avaliar a influência do biocarvão produzido a partir do resíduo do fruto do cafeeiro, no desenvolvimento de mudas de alface quando adicionado a substrato comercial, foi conduzido experimento em casa de vegetação, com delineamento experimental inteiramente casualizado, com cinco tratamentos e oito repetições. Utilizou-se a cultivar Grandes Lagos (Lactuca sativa L.) e cinco substratos, sendo um comercial, Bioplant® (PLT) e quatro formados a partir do substrato comercial, Bioplant® + biocarvão (BC 5, BC 10, BC 15 e BC 25). Foram avaliadas as variáveis percentagem de emergência (E), índice de velocidade de emergência (IVE), comprimento parte aérea (CPA) e sistema radicular (CSR), massa fresca da parte aérea (MFPA) e do sistema radicular (MFSR) e massa seca da parte aérea (MSPA) e do sistema radicular (MSSR), 25 dias após a semeadura. Os tratamentos, BC 5, BC 10, BC 15, apresentaram 100% de plântulas emergidas e IVE de 37,1 Plântulas dia-1; 37,5 Plântulas dia-1 e 37,7 Plântulas dia-1, respectivamente, resultados comparáveis ao tratamento PLT. O tratamento BC 10, apresentou maiores valores para as variáveis CPA, CSR, MFPA, MFSR, MSPA e MSSR (2,38 cm; 5,16 cm; 89,1 mg; 88,8 mg; 12,7 mg; 10,3 mg, respectivamente), diferenciando-se significativamente do tratamento PLT. A adição de biocarvão (10 % em massa), foi uma alternativa viável para ser utilizado como condicionador do substrato comercial, Bioplant®, para produção de mudas de alface da cultivar Grandes Lagos.
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Berth, Mario, and Eugene Bosmans. "Comparison of Three Automated Immunoassay Methods for the Determination of Epstein-Barr Virus-Specific Immunoglobulin M." Clinical and Vaccine Immunology 17, no. 4 (February 10, 2010): 559–63. http://dx.doi.org/10.1128/cvi.00372-09.

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ABSTRACT In this study we compared the performances of three commercially available Epstein-Barr virus (EBV) immunoglobulin M (IgM) assays on highly automated immunoassay platforms: BioPlex 2200 (Bio-Rad Laboratories), Immulite 2000 (Siemens Healthcare Diagnostics), and Liaison (DiaSorin). As a confirmatory method, immunoblotting was performed. The specificity of the three EBV IgM assays was evaluated by testing 293 selected sera from patients with various infectious and noninfectious diseases. After the exclusion of 30 samples, the specificities were 96.2% for Liaison, 98.1% for Immulite, and 97.0% for BioPlex. For evaluation of the sensitivity, samples from 70 consecutive patients with a positive heterophile antibody test were examined, irrespective of clinical or biological findings. After the exclusion of six samples, the sensitivities were 89.1% for Liaison, 84.4% for Immulite, and 89.1% for BioPlex. Finally, in a prospective study performed with 500 samples obtained from consecutive patients and sent in by general practitioners, we also determined Epstein-Barr nuclear antigen IgG and viral capsid antigen IgG in a two-phase approach. Concordance of the EBV serologic status was 96.2% between Liaison and Immulite, 96.4% between Immulite and BioPlex, and 97.8% between BioPlex and Liaison. The three EBV IgM immunoassays that we evaluated have acceptable and comparable performances.
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Sammons, Jonathan D., and Daniel K. Struve. "Effect of Bioplex™ on Transplant Success of Non-Dormant Red Oak (Quercus rubra L.)." Journal of Environmental Horticulture 22, no. 4 (December 1, 2004): 197–201. http://dx.doi.org/10.24266/0738-2898-22.4.197.

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Abstract Biostimulants are used to reduce the stress associated with non-dormant (summer dug) harvest of field-grown nursery stock; however, the effectiveness of biostimulant treatment is uncertain. This study tested the effects of three application methods of Bioplex™ (a commonly used biostimulant) to container-grown red oak seedlings on whole plant transpirational water use and growth before and after root pruning. Root pruning was used to simulate field harvest; it removed 59% of the seedling's total root surface area. Bioplex™ application by foliar spray, soil drench or a combination of foliar spray and soil drench, significantly reduced whole plant transpirational water use by 15% for three days after application, relative to untreated control seedlings. Root pruning significantly reduced whole plant transpiration, compared to non-root-pruned seedlings, and had a greater effect on transpiration than any Bioplex™ treatment. The previous season's Bioplex treatment had no effect on the spring growth flush following fall root pruning. Root pruning in fall significantly reduced root and total plant dry weights the following spring. Although Bioplex™ applications significantly reduced transpiration for three days after application, there does not seem to be any long-term beneficial effect when used to mediate summer digging transplant stress.
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Cottin, H., Y. Y. Guan, P. Coll, D. Coscia, N. Fray, F. Macari, F. Stalport, et al. "Astrochemistry on the EXPOSE/ISS and BIOPAN/Foton experiments." Proceedings of the International Astronomical Union 5, H15 (November 2009): 684–85. http://dx.doi.org/10.1017/s174392131001094x.

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41

Yilmaz, R. Burcu Nur, Meltem Mutlu, and Derya Germec-Cakan. "Clear double layer Bioplast feeding plate for neonates with cleft palate." European Journal of Dentistry 09, no. 04 (October 2015): 610–13. http://dx.doi.org/10.4103/1305-7456.172629.

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ABSTRACTAn infant with cleft palate was referred to cleft clinic of the Orthodontic Department. The mother was concerned to feed the child because of the escape of milk from the nose. Intraoral examination revealed a large palatal cleft extending from hard to soft palate involving uvula. The impression was taken and dental cast obtained. A 3 mm soft and afterward a 1 mm hard Bioplast plate was pressed using Biostar device (Scheu-Dental Gmbh, Iserlohn, Germany) on the model. Finally, a hole was prepared on the anterior part to put a thread through it. The infant tolerated the plate immediately and encounters no difficulties during feeding. The inlaying soft Bioplast plates eliminate the risk of tissue irritation, whereas the covering hard Bioplast plate supplies endurance. The fabrication of the clear Bioplast feeding plate is easy and less time-consuming compared with acrylic plates and may be recommended in crowded and overloaded cleft centers.
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Marangoni, Antonella, Paola Nardini, Claudio Foschi, Alessandra Moroni, Antonietta D'Antuono, Letizia Bacchi Reggiani, and Roberto Cevenini. "Evaluation of the BioPlex 2200 Syphilis System as a First-Line Method of Reverse-Sequence Screening for Syphilis Diagnosis." Clinical and Vaccine Immunology 20, no. 7 (May 22, 2013): 1084–88. http://dx.doi.org/10.1128/cvi.00316-13.

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ABSTRACTDespite recent technological advances, the diagnosis of syphilis remains a challenging enterprise. Actually, most high-volume laboratories have adopted the “reverse algorithm” due several factors, including the potential to automate testing. Recently, immunoassays processed on random-access systems have been proposed as screening tests. The purpose of this study was to evaluate diagnostic performances of BioPlex 2200 Syphilis IgG and BioPlex 2200 Syphilis IgM, tests based on Multiplex Flow technology, in comparison with the performance of Architect Syphilis TP, a chemiluminescent immunoassay for the detection of IgG and/or IgM anti-Treponema pallidumantibodies. A retrospective study was performed with a panel of 100 blood donor sera, a panel of 350 clinical and laboratory-characterized syphilitic sera, and 170 samples obtained from subjects with potentially interfering conditions. Moreover, 200 unselected samples submitted to the Microbiology Laboratory of St. Orsola Hospital in Bologna for routine screening for syphilis were evaluated. As confirmatory tests,T. pallidumhemagglutination and Western blot assays were used. Considering the IgG Western blot (WB) assay to be the gold standard method, BioPlex 2200 Syphilis IgG specificity was far higher than Architect Syphilis TP specificity (89.7% versus 78.4%, respectively), whereas the sensitivity was 100% for both automated methods. Compared to the IgM WB assay, BioPlex 2200 Syphilis IgM performed with a specificity of 94.9%, whereas the sensitivity was 84.8%. Considering the excellent ease of use and automation, the high sample throughput and its valuable analytical performances, BioPlex Syphilis 2200 IgG could represent a suitable choice for high-volume laboratories. BioPlex Syphilis 2200 IgM could be considered a good addition to IgG testing for uncovering active infections.
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Guigue, Nicolas, Jean Menotti, Samia Hamane, Francis Derouin, and Yves Jean-François Garin. "Performance of the BioPlex 2200 Flow Immunoassay in Critical Cases of Serodiagnosis of Toxoplasmosis." Clinical and Vaccine Immunology 21, no. 4 (January 29, 2014): 496–500. http://dx.doi.org/10.1128/cvi.00624-13.

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ABSTRACTThe BioPlex 2200 automated analyzer (Bio-Rad Laboratories, Hercules, CA) is a recently developed multiplex analyzer that enables the detection of anti-Toxoplasma, -rubella, and -cytomegalovirus antibodies in the same assay. The aim of this study was to compare this new technology (using the BioPlex 2200 ToRC IgG/IgM kit) in critical cases of serodiagnosis of toxoplasmosis (acute, chronic, or congenital infections and cases with discrepant results) to the technologies used in our routine practice, i.e., the Platelia IgG/IgM enzyme-linked immunosorbent assays (ELISAs) (Bio-Rad Laboratories) and the Toxo-Screen direct agglutination assay (bioMérieux, Lyon, France). Overall, most cases of false-positive/negative results obtained with the Platelia IgG or Toxo-Screen assay were corrected by the BioPlex 2200 ToRC IgG (87.5%). Furthermore, the analysis of 35 sequences of sera showed a trend toward a more rapid decrease of IgM titers by BioPlex 2200 than by Platelia. These results for IgM detection can be explained by a weaker detection of residual IgM. Indeed, among 23 serum samples from patients with probable past infection with long-lasting IgM (Platelia M positive and IgG avidity index, ≥0.5), the BioPlex 2200ToxoplasmaIgM assay was positive for only 11 serum samples. In our panel of critical cases comprising 156 serum and 6 cord blood samples from 103 patients with acute, chronic, or congenital infection, the BioPlex 2200 IgG assay was a sensitive (97.8%) and specific (91.3%) method for IgG detection. The high specificity (97.4%) of IgM detection combined with the shorter kinetics of IgM titers may considerably reduce the number of residual IgM detections, thus yielding more precise diagnoses of acute infections.
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Chatelain, Bernard, Christian Chatelain, Pierre Wallemacq, Jean-Michel Dogné, Jonathan Douxfils, Anne Tamigniau, and François Mullier. "Comparison of calibrated chromogenic anti-Xa assay and PT tests with LC-MS/MS for the therapeutic monitoring of patients treated with rivaroxaban." Thrombosis and Haemostasis 110, no. 10 (2013): 723–31. http://dx.doi.org/10.1160/th13-04-0274.

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SummaryPossibilities to monitor rivaroxaban therapy could be useful in certain circumstances. Prothrombin time (PT) or chromogenic anti-Xa assays such as the Biophen Direct Factor Xa Inhibitor® (DiXaI) have been proposed to estimate rivaroxaban concentrations but are mainly based on in vitro studies. The study aim was to compare PT and Biophen DiXaI® measurements with liquid chromatography-tandem mass spectrometry (LC-MS/MS) measurements in plasma samples from patients treated with Xarelto®. Fifty-two plasma samples were included. PT was performed using Innovin® and Triniclot PT Excel S®. Biophen DiXaI® was performed according to instructions from the manufacturer. The rivaroxaban plasma concentration ranged between 0 and 485 ng/ml as measured by LC-MS/MS. The limits of quantification were 30 ng/ml and 5 ng/ml for Biophen DiXaI® and LC-MS/MS, respectively. The linear correlation between Biophen DiXaI® and LC-MS/ MS analyses was high for all rivaroxaban concentrations (r2 = 0.95). For concentrations ≤100 ng/ml, r2-value was 0.83. The Bland-Altman analysis showed a mean difference of −16 ng/ml (SD: 25 ng/ml). The PT methods did not correlate well with plasma concentrations measured by LC-MS/MS (r2 ≈ 0.60). In conclusion, the important interindividual variability and the poor correlation with LC-MS/MS preclude the use of PT to estimate rivaroxaban concentrations. Thanks to its small inter-individual variability and good agreement with LC-MS/ MS measurements, we recommend the use of Biophen DiXaI® assays to estimate concentrations of rivaroxaban >30 ng/ml. Quantification of low rivaroxaban levels (<30 ng/ml) requires the LC-MS/MS method.
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Mi, Huaiyu, Anushya Muruganujan, Emek Demir, Yukiko Matsuoka, Akira Funahashi, Hiroaki Kitano, and Paul D. Thomas. "BioPAX support in CellDesigner." Bioinformatics 27, no. 24 (October 21, 2011): 3437–38. http://dx.doi.org/10.1093/bioinformatics/btr586.

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Borges, Kelly Cristiene de Freitas, Denise Garcia de Santana, Susana Webber Lopes, and Vanderley José Pereira. "Coloração do Fruto e Substrato na Emergência e no Crescimento de Plantas de Eugenia calycina Cambess." Floresta e Ambiente 23, no. 4 (April 29, 2016): 544–54. http://dx.doi.org/10.1590/2179-8087.144215.

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RESUMO O objetivo do trabalho foi avaliar a influência da coloração dos frutos na emergência e no crescimento das plantas de Eugenia calycina Cambess (pitangueira-do-cerrado), assim como de substratos com diferentes proporções de material orgânico e estruturante. Três experimentos foram instalados: o primeiro de emergência; o segundo de crescimento de plantas constituídos de sementes provenientes de frutos verdes, alaranjados/avermelhados, vermelho-claros e vermelho-escuros; e o terceiro comparou cinco misturas de Bioplant® suplementado com Vermiculita® e com pó-de-coco no crescimento das plantas, ambos nas proporções de 20% e 40%. A coloração do fruto não influenciou a capacidade de emergência, tempo (início, médio e final), velocidade e sincronia de emergência das plântulas de E. calycina. O crescimento das plantas até 270 dias de cultivo foi independente da coloração do fruto. A Vermiculita® e o pó-de-coco suplementados ao Bioplant® não afetaram o crescimento das plantas até os 120 dias de cultivo.
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Guerra, Mariana Santana, Marcelo Sousa Barbosa, Edilson Costa, and Gustavo Haralampidou Costa Vieira. "RECIPIENTE BIODEGRADÁVEL E SUBSTRATOS PARA MUDAS DE MARACUJAZEIRO." JOURNAL OF NEOTROPICAL AGRICULTURE 4, no. 3 (August 31, 2017): 50–54. http://dx.doi.org/10.32404/rean.v4i3.1641.

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O descarte incorreto dos recipientes plásticos usados na produção de mudas causa impactos ao ambiente. O maracujazeiro amarelo é produzido em grande escala, tendo importância fármaco-terapêutico, além de ser uma frutífera de produção rápida, sendo para isso imprescindível a obtenção de mudas de qualidade. O objetivo deste trabalho foi avaliar recipientes e substratos na formação de mudas de maracujazeiro. O experimento foi conduzido num delineamento inteiramente casualizado, em esquema fatorial 2 x 4, sendo 2 recipientes e 4 substratos. Como recipientes foram utilizados bucha vegetal e sacos plásticos. Em cada recipiente foram testados os seguintes substratos: 30% de esterco bovino + 70% de vermiculita; 30% de Bioplant + 70% vermiculita; 30% de esterco bovino + 70% de areia; 30% de Bioplant + 70% areia. Foram avaliadas variáveis referentes a altura, diâmetro do colo, fitomassas e relações biométricas. As mudas mais vigorosas de maracujazeiro foram obtidas com o recipiente de polietileno. Para os substratos, o melhor resultado foi observado utilizando-se a mistura de esterco e vermiculita.
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Endo, Isao, Tsuyoshi Suzuki, Yoshito Sakino, Teruo Fujii, Hayato Kaetsu, and Hajime Asama. "An Adaptive Multirobot Scheduling System for Maintenance of a Bioplant." IFAC Proceedings Volumes 31, no. 8 (May 1998): 233–38. http://dx.doi.org/10.1016/s1474-6670(17)40191-1.

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49

Gomez, E., D. J. Jespersen, J. A. Harring, and M. J. Binnicker. "Evaluation of the Bio-Rad BioPlex 2200 Syphilis Multiplex Flow Immunoassay for the Detection of IgM- and IgG-Class Antitreponemal Antibodies." Clinical and Vaccine Immunology 17, no. 6 (April 28, 2010): 966–68. http://dx.doi.org/10.1128/cvi.00086-10.

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ABSTRACT The laboratory diagnosis of syphilis is based primarily upon serologic findings. Historically, serologic testing for syphilis has relied on assays such as rapid plasma reagin, fluorescent treponemal antibody absorption, Treponema pallidum particle agglutination (TP-PA), and more recently, enzyme immunoassay (EIA). In this study, we evaluated the performance of a novel multiplex flow immunoassay (BioPlex 2200 Syphilis; Bio-Rad Laboratories, Hercules, CA) for the detection of antitreponemal IgG- and IgM-class antibodies. Serum specimens (n = 1,008) submitted for routine treponema-specific antibody testing by syphilis IgM and IgG EIA (Trep-Chek; Phoenix-Biotech, Mississauga, Ontario, Canada) were also analyzed by the BioPlex Syphilis multiplex assay. Specimens showing discordant results were repeat tested, with further discrepancies being arbitrated by TP-PA. Compared directly to the results of EIA, the BioPlex IgG assay demonstrated 98.7% (77/78) sensitivity and 99.4% (916/930) specificity. Compared to the Trep-Chek IgM EIA, the BioPlex IgM assay showed 80% (4/5) sensitivity and 97.9% (652/666) specificity. These results indicate that the BioPlex Syphilis multiplex assay shows similar serological agreement with EIA while allowing for a fully automated random-access platform that provides faster (1.7 h for 100 samples versus 4.5 h by EIA) and higher-throughput (800 samples per 9 h versus 200 samples by EIA) analysis of the syphilis serologic response.
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50

Dutra, Tiago Reis, Paulo Henrique Grazziotti, Reynaldo Campos Santana, and Marília Dutra Massad. "QUALIDADE DE MUDAS DE COPAÍBA PRODUZIDAS EM DIFERENTES SUBSTRATOS E NÍVEIS DE SOMBREAMENTO." FLORESTA 45, no. 3 (March 18, 2015): 635. http://dx.doi.org/10.5380/rf.v45i3.35686.

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O presente trabalho teve como objetivo avaliar a influência de dois volumes de diferentes substratos e níveis de sombreamento sobre a qualidade de mudas de copaíba. O experimento foi conduzido por 130 dias em DBC casualizados no esquema fatorial 2 x 5, sendo avaliados dois volumes (180 e 280 cm³) dos substratos Bioplant®; 70% vermiculita + 30% casca de arroz carbonizada; 40% vermiculita + 30% casca de arroz carbonizada + 30% fibra de côco; 50% vermiculita + 30% casca de arroz carbonizada + 20% areia; e 70% vermiculita + 15% casca de arroz carbonizada + 15% vermicomposto. Essas dez combinações foram distribuídas aleatoriamente em quatro blocos com diferentes intensidades luminosas: 0, 30, 50 e 70% de sombreamento. O uso de 180 cm³ de substrato foi suficiente para produzir mudas de copaíba com bom desenvolvimento e índice de qualidade. Os substratos 70V+30CA, 40V+30CA+30FC, 50V+30CA+20A, 70V+15CA+15VC apresentaram-se superiores em relação ao Bioplant® para produção de mudas da espécie em estudo. Os resultados demonstraram que as mudas de copaíba comportaram-se de forma distinta em relação aos níveis de sombreamento avaliados, demonstrando possuírem grande plasticidade adaptativa.AbstractQuality of copaiba seedlings produced in two volumes of substrates and shading levels. This research aimed to evaluate the influence of two volumes of substrate and levels of shading on seedling quality of copaiba. The experiment was conducted for 130 days in a randomized block design in a 2 x 5 factorial scheme, we evaluated two volumes (180 e 280 cm³) of the five following substrates: Bioplant®, 70% vermiculite + 30% carbonized rice hulls, 40% vermiculite + 30% carbonized rice hulls + 30% coir, 50% vermiculite + 30% carbonized rice hulls + 20% sand and 70% vermiculite + 15% carbonized rice hulls + 15% vermicompost residue textiles. These ten combinations were randomly distributed in four blocks, accordingly to the following distinct brightness: 0, 30, 50 and 70 percentages of shading. The use of 180 cm³ substrate was enough to produce seedlings of copaiba with good development and quality index. The substrates 70V+30CA, 40V+30CA+30FC, 50V+30CA+20A, and 70V+15CA+15VC showed slight superiority over Bioplant® for seedlings of such species. The results revealed that the copaiba seedlings behaved differently in relation to shading levels evaluated, it demonstrates they have great adaptive plasticity.Keywords: Copaifera langsdorffii; production of seedlings; levels of brightness; containers.
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