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Journal articles on the topic 'Biopolymers; PHB synthesis in bacteria'

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Published: 4 June 2021

Last updated: 3 February 2022

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1

Mostafa, Yasser S., Sulaiman A. Alrumman, Kholod A. Otaif, Saad A. Alamri, Mohamed S. Mostafa, and Taher Sahlabji. "Production and Characterization of Bioplastic by Polyhydroxybutyrate Accumulating Erythrobacter aquimaris Isolated from Mangrove Rhizosphere." Molecules 25, no. 1 (2020): 179. http://dx.doi.org/10.3390/molecules25010179.

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The synthesis of bioplastic from marine microbes has a great attendance in the realm of biotechnological applications for sustainable eco-management. This study aims to isolate novel strains of poly-β-hydroxybutyrate (PHB)-producing bacteria from the mangrove rhizosphere, Red Sea, Saudi Arabia, and to characterize the extracted polymer. The efficient marine bacterial isolates were identified by the phylogenetic analysis of the 16S rRNA genes as Tamlana crocina, Bacillus aquimaris, Erythrobacter aquimaris, and Halomonas halophila. The optimization of PHB accumulation by E. aquimaris was achieve

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Upadhayay, Varsha, Samakshi Verma, and Arindam Kuila. "Production of poly hydroxy butyrate (PHB) from Eichhornia crassipes through microbial fermentation process." Plant Science Today 6, sp1 (2019): 541–50. http://dx.doi.org/10.14719/pst.2019.6.sp1.673.

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Polyhydroxybutyrate (PHB) is one of the highly biodegradable and biologically acceptable thermoplastics synthesized by many microorganisms collectively called polyhydroxyalkanoates (PHAs). All available biopolymers are viewed as perfect answers for the resolution of natural contamination issue by supplanting ordinary plastic business. They are likewise utilized as osteosclerotic stimulants attributable to their piezoelectric properties, in bone plates, during operations as suture material and vein substitutions. Synthesis of PHB is found in a wide range of Gram’s negative and gram’s positive b

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Montazer, Zahra, Mohammad B. Habibi Najafi, and David B. Levin. "Microbial degradation of low-density polyethylene and synthesis of polyhydroxyalkanoate polymers." Canadian Journal of Microbiology 65, no. 3 (2019): 224–34. http://dx.doi.org/10.1139/cjm-2018-0335.

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We have characterized the ability of eight bacterial strains to utilize powdered low-density polyethylene (LDPE) plastic (untreated and without any additives) as a sole carbon source. Cell mass production on LDPE-containing medium after 21 days of incubation varied between 0.083 ± 0.015 g/L cell dry mass (cdm) for Micrococcus luteus IRN20 and 0.39 ± 0.036 g/L for Cupriavidus necator H16. The percent decrease in LDPE mass ranged from 18.9% ± 0.72% for M. luteus IRN20 to 33.7% ± 1.2% for C. necator H16. Linear alkane hydrolysis products from LDPE degradation were detected in the culture media, a

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4

Thapa, Christina, Pallavi Shakya, Rabina Shrestha, Sushovita Pal, and Prakash Manandhar. "Isolation of Polyhydroxybutyrate (PHB) Producing Bacteria, Optimization of Culture Conditions for PHB production, Extraction and Characterization of PHB." Nepal Journal of Biotechnology 6, no. 1 (2019): 62–68. http://dx.doi.org/10.3126/njb.v6i1.22339.

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Polyhydroxybutyrates (PHBs) are energy reserves synthesized by different micro-organisms such as Alcaligenes, Pseudomonas, Staphylococcus, Algae, in excess of carbon and limitation of nutrients like nitrogen. These biopolymers are suitable alternate to synthetic carbon-based polymers. However, the high production cost limits their commercialization. The aim of this study was thus, focused on optimization of culture condition for maximum PHB production in an attempt to reduce the production cost. The micro-organisms for this purpose were isolated from 4 different soil samples and screened for P

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Orts, William J., Geoffrey A. R. Nobes, Jumpei Kawada, Sophie Nguyen, Ga-er Yu, and François Ravenelle. "Poly(hydroxyalkanoates): Biorefinery polymers with a whole range of applications. The work of Robert H. Marchessault." Canadian Journal of Chemistry 86, no. 6 (2008): 628–40. http://dx.doi.org/10.1139/v08-050.

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This review describes the characterization and application of poly(hydroxyalkanoates), PHAs, a remarkable family of natural polyesters with a wide array of useful properties and potential applications. It places specific emphasis on the work of Robert H. Marchessault and his many colleagues outlining how Marchessault’s body of work both shaped the field and complemented the work of his contemporaries. Particular attention will focus on the “rediscovery” of poly(β-hydroxybutyrate), PHB, the first PHA to be discovered, from the late 1950s onward, highlighting some of the historical aspects of PH

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Saratale, Rijuta Ganesh, Si-Kyung Cho, Gajanan S. Ghodake, et al. "Utilization of Noxious Weed Water Hyacinth Biomass as a Potential Feedstock for Biopolymers Production: A Novel Approach." Polymers 12, no. 8 (2020): 1704. http://dx.doi.org/10.3390/polym12081704.

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This study aims to utilize a noxious weed water hyacinth biomass (WH) for polyhydroxybutyrate (PHB) production. Alkaline and peracetic acid pretreatment was employed for the hydrolysis of WH and consequently enzymatic saccharification to produce fermentable sugars for PHB production. The pretreatment competence was determined using various operational parameters. By applying ambient conditions, alkaline pretreatment gave higher lignin removal of 65.0%, with 80.8% hydrolysis yield, and on enzyme hydrolysis (40 FPU/g of dry WH), produced total reducing sugar of about 523 mg/g of WH. The resulted

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7

Aragosa, Amina, Valeria Specchia, and Mariaenrica Frigione. "Isolation of Two Bacterial Species from Argan Soil in Morocco Associated with Polyhydroxybutyrate (PHB) Accumulation: Current Potential and Future Prospects for the Bio-Based Polymer Production." Polymers 13, no. 11 (2021): 1870. http://dx.doi.org/10.3390/polym13111870.

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The environmental issues caused by the impacts of synthetic plastics use and derived wastes are arising the attention to bio-based plastics, natural polymers produced from renewable resources, including agricultural, industrial, and domestic wastes. Bio-based plastics represent a potential alternative to petroleum-based materials, due to the insufficient availability of fossil resources in the future and the affordable low cost of renewable ones that might be consumed for the biopolymer synthesis. Among the polyhydroxyalkanoates (PHA), the polyhydroxybutyrate (PHB) biopolymer has been synthesi

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Pramanik, J., P. L. Trelstad, and J. D. Keasling. "A flux-based stoichiometric model of enhanced biological phosphorus removal metabolism." Water Science and Technology 37, no. 4-5 (1998): 609–13. http://dx.doi.org/10.2166/wst.1998.0727.

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Enhanced biological phosphorus removal (EBPR) in wastewater treatment involves metabolic cycling through the biopolymers polyphosphate (polyP), polyhydroxybutyrate (PHB), and glycogen. This cycling is induced through treatment systems that alternate between carbon-rich anaerobic and carbon-poor aerobic reactor basins. While the appearance and disappearance of these biopolymers has been documented, the intracellular pressures that regulate their synthesis and degradation are not well understood. Current models of the EBPR process have examined a limited number of metabolic pathways that are fre

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9

Castillo-Arteaga, Roger David, Edith Mariela Burbano-Rosero, Iván Dario Otero-Ramirez, Juan Camilo Roncallo, Sandra Patricia Hidalgo-Bonilla, and Pablo Fernández-Izquierdo. "Polyhydroxyalkanoate biosynthesis by oxalotrophic bacteria from high Andean soil." Universitas Scientiarum 23, no. 1 (2018): 35. http://dx.doi.org/10.11144/javeriana.sc23-1.pbbo.

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<p class="ABSTRACT">Oxalate is a highly oxidized organic acid anion used as a carbon and energy source by oxalotrophic bacteria. Oxalogenic plants convert atmospheric CO2 into oxalic acid and oxalic salts. Oxalate-salt formation acts as a carbon sink in terrestrial ecosystems via the oxalate-carbonate pathway (OCP). Oxalotrophic bacteria might be implicated in other carbon-storage processes, including the synthesis of polyhydroxyalkanoates (PHAs). More recently, a variety of bacteria from the Andean region of Colombia in Nariño have been reported for their PHA-producing abilities. These

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Peregrina, Alexandra, João Martins-Lourenço, Filomena Freitas, Maria A. M. Reis, and Cecília M. Arraiano. "Post-Transcriptional Control in the Regulation of Polyhydroxyalkanoates Synthesis." Life 11, no. 8 (2021): 853. http://dx.doi.org/10.3390/life11080853.

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The large production of non-degradable petrol-based plastics has become a major global issue due to its environmental pollution. Biopolymers produced by microorganisms such as polyhydroxyalkanoates (PHAs) are gaining potential as a sustainable alternative, but the high cost associated with their industrial production has been a limiting factor. Post-transcriptional regulation is a key step to control gene expression in changing environments and has been reported to play a major role in numerous cellular processes. However, limited reports are available concerning the regulation of PHA accumula

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Heinrich, Daniel, Matthias Raberg, Philipp Fricke, et al. "Synthesis Gas (Syngas)-Derived Medium-Chain-Length Polyhydroxyalkanoate Synthesis in Engineered Rhodospirillum rubrum." Applied and Environmental Microbiology 82, no. 20 (2016): 6132–40. http://dx.doi.org/10.1128/aem.01744-16.

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ABSTRACTThe purple nonsulfur alphaproteobacteriumRhodospirillum rubrumS1 was genetically engineered to synthesize a heteropolymer of mainly 3-hydroxydecanoic acid and 3-hydroxyoctanoic acid [P(3HD-co-3HO)] from CO- and CO2-containing artificial synthesis gas (syngas). For this, genes fromPseudomonas putidaKT2440 coding for a 3-hydroxyacyl acyl carrier protein (ACP) thioesterase (phaG), a medium-chain-length (MCL) fatty acid coenzyme A (CoA) ligase (PP_0763), and an MCL polyhydroxyalkanoate (PHA) synthase (phaC1) were cloned and expressed under the control of the CO-inducible promoter PcooFfrom

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B H, Nanjunda Reddy, Prdadipta Ranjan Rauta, Venkatalakshimi V, and Swamy Sreenivasa. "SYNTHESIS AND CHARACTERIZATION OF NOVEL SA-PA-LSA/C-30B/AG NANOCOMPOSITES FOR SWELLING, ANTIBACTERIAL, DRUG DELIVERY, AND ANTICANCER APPLICATIONS." Asian Journal of Pharmaceutical and Clinical Research 11, no. 3 (2018): 229. http://dx.doi.org/10.22159/ajpcr.2018.v11i3.22939.

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Objective: The main objective of this work was to formulate and evaluate Closite-30B/nanoAg filled hydrogel composites which are further intentended to be used for the study of drug delivery,antibacterial, and anticancer activityMethods: In this study, Cloisite-30B (C-30B) clay dispersed biopolymer sodium alginate (SA)-grafted-poly (acrylamide [AAm]-co-lignosulfonic acid) hydrogel composites were synthesized by free radical in situ polymerization reaction technique using SA, AAm, and lignosulfonic acid biopolymers in different proportions in combination. which are subjected to invitro drug del

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13

Mansfield, David A., Alistair J. Anderson, and Linda A. Naylor. "Regulation of PHB metabolism inAlcaligenes eutrophus." Canadian Journal of Microbiology 41, no. 13 (1995): 44–49. http://dx.doi.org/10.1139/m95-166.

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Metabolites associated with the poly(3-hydroxybutyrate) (PHB) biosynthetic pathway in Alcaligenes eutrophus were measured to gain an insight into the regulation of PHB synthesis in vivo. Alcaligenes eutrophus was grown in carbon-limited chemostat culture to provide bacteria producing negligible PHB, and in nitrogen-limited chemostat culture to yield PHB-synthesizing bacteria. 3-Hydroxybutyryl-CoA (3HBCoA) was detected only in polymer-accumulating bacteria. The level of coenzyme A (CoASH) was approximately three times higher in the absence of PHB synthesis, in accord with the putative role of t

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14

Wang, He Teng, and Jing Dong. "The Potential of Methane-Oxidizing Bacteria for Applications in the Synthesis of Green Material-PHB." Applied Mechanics and Materials 200 (October 2012): 385–88. http://dx.doi.org/10.4028/www.scientific.net/amm.200.385.

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The current status of biodegradable material PHB research was analyzed. The synthesis mechanism of PHB production by methane-oxidizing bacteria and effect factors of PHB yield were summarized. And progress direction about PHB polyester was forecasted, that provide theoretic base for studying PHB.

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15

Gao, Shuang, та Ling Hua Zhang. "The Synthesis of Poly-β-Hydroxybutyrate by Moderately Halophilic Bacteria Halomonas venusta". Advanced Materials Research 1033-1034 (жовтень 2014): 306–10. http://dx.doi.org/10.4028/www.scientific.net/amr.1033-1034.306.

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In this study, poly-β-hydroxybutyrate (PHB) was synthesized by moderately halophilic bacteria Halomonas venusta and identified by 1H-NMR. The effects of different carbon sources, salt concentration, initial carbon nitrogen quality ratio, initial phosphate concentration were studied on the synthesis of PHB. The optimum conditions of the PHB synthesis were detemined. Glucose was as carbon source with the concentration of 80 g/L and salt concentration, initial carbon nitrogen ratio, initial phosphate concentration was 3%, 15 and 1.9 g/L respectively. Under the above conditions, a two-phase synthe

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16

Yanti, Nur Arfa, Langkah Sembiring, Sebastian Margino та Nurhayani H. Muhiddin. "A Study on Production of Poly-β-Hydroxybutyrate Bioplastic from Sago Starch by Indigenous Amylolytic Bacteria". Indonesian Journal of Biotechnology 18, № 2 (2015): 144. http://dx.doi.org/10.22146/ijbiotech.7877.

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Bacillus sp. PSA10 and Bacillus sp. PPK5 were two indigenous strain amylolytic bacteria from SoutheastSulawesi that have ability to produce bioplastic poly-β-hydroxybutyrate (PHB) from sago starch. The study wasattempted to determine the mechanism of PHB production by bacteria amylolytic was grown on sago starchcontainingmedia. Two amylolytic bacteria i.e. Bacillus sp. PSA10 and Bacillus sp. PPK5 was grown for 168 hin a mineral salts medium with sago starch as carbon source. Growth of amylolytic bacteria was monitoredby cell dry weight. Extraction of PHB was done by N-hexane acetone-diethyl et

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Parodi, Adriano, Alexandra Jorea, Maurizio Fagnoni, et al. "Bio-based crotonic acid from polyhydroxybutyrate: synthesis and photocatalyzed hydroacylation." Green Chemistry 23, no. 9 (2021): 3420–27. http://dx.doi.org/10.1039/d1gc00421b.

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Bio-based crotonic acid was prepared via the thermolytic distillation of polyhydroxybutyrate (PHB) and PHB-enriched bacteria, and then subjected to a photocatalyzed hydroacylation, showing a comparable reactivity with that of commercial crotonic acid.

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Zaki, Dr Neihaya Heikmat. "Biodegradable plastic production by Bacillus spp. isolated from agricultural wastes and genetic analysis of PHA synthesis." Al-Mustansiriyah Journal of Science 29, no. 1 (2018): 67. http://dx.doi.org/10.23851/mjs.v29i1.115.

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Abstract Polyhydroxyalkanoates (PHAs) are resources that hoard by a variety of microorganisms as nutrient source. It is environmentally recyclable biopolymer, and totally corrupted biologically to simple molecules. A total of 38 isolates from agricultural wastes, 9 isolates exhibited optimistic outcome (dark blue particles) for PHA accumulation when stain by Sudan black B. Maximum bacterial growth was achieved at temperature 35°C, PH 7, (48-72) hr incubation and 0.25% concentration of NaCl. Chosen isolate (NE1MOK) recognized as Bacillus subtilis when diagnosed by traditional and molecular meth

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Szacherska, Karolina, Piotr Oleskowicz-Popiel, Slawomir Ciesielski, and Justyna Mozejko-Ciesielska. "Volatile Fatty Acids as Carbon Sources for Polyhydroxyalkanoates Production." Polymers 13, no. 3 (2021): 321. http://dx.doi.org/10.3390/polym13030321.

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Waste of industrial origin produced from synthetic materials are a serious threat to the natural environment. The ending resources of fossil raw materials and increasingly restrictive legal standards for the management of plastic waste have led to research on the use of biopolymers, which, due to their properties, may be an ecological alternative to currently used petrochemical polymers. Polyhydroxyalkanoates (PHAs) have gained much attention in recent years as the next generation of environmentally friendly materials. Currently, a lot of research is being done to reduce the costs of the biolo

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Ghosh, Sreejita, Dibyajit Lahiri, Moupriya Nag, et al. "Bacterial Biopolymer: Its Role in Pathogenesis to Effective Biomaterials." Polymers 13, no. 8 (2021): 1242. http://dx.doi.org/10.3390/polym13081242.

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Bacteria are considered as the major cell factories, which can effectively convert nitrogen and carbon sources to a wide variety of extracellular and intracellular biopolymers like polyamides, polysaccharides, polyphosphates, polyesters, proteinaceous compounds, and extracellular DNA. Bacterial biopolymers find applications in pathogenicity, and their diverse materialistic and chemical properties make them suitable to be used in medicinal industries. When these biopolymer compounds are obtained from pathogenic bacteria, they serve as important virulence factors, but when they are produced by n

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Schlebusch, Maximilian, and Karl Forchhammer. "Requirement of the Nitrogen Starvation-Induced Protein Sll0783 for Polyhydroxybutyrate Accumulation in Synechocystis sp. Strain PCC 6803." Applied and Environmental Microbiology 76, no. 18 (2010): 6101–7. http://dx.doi.org/10.1128/aem.00484-10.

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ABSTRACT Nitrogen is often a limiting nutrient in natural habitats. Therefore, cyanobacteria have developed multiple responses, which are controlled by transcription factor NtcA and the PII-signaling protein, to adapt to nitrogen deficiency. Transcriptional analyses of Synechocystis sp. strain PCC 6803 under nitrogen-deficient conditions revealed a highly induced gene (sll0783) which is annotated as encoding a conserved protein with an unknown function. This gene is part of a cluster of seven genes and has potential NtcA-binding sites in the upstream region. Homologues of this cluster occur in

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Talanyuk, V., A. Shadrin, M. Iurzhenko, N. Korab, and M. Menzheres. "WELDING PROCESS AND FEATURES OF MICROSTRUCTURE OF WELDED JOINTS OF BIOPOLYMERS." Innovative Solution in Modern Science 1, no. 37 (2020): 48. http://dx.doi.org/10.26886/2414-634x.1(37)2020.4.

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Polymers and biopolymers in particular are increasingly used in various sectors of the economy and more recently biopolymers replace traditional polymers in many applications. The problem of polymeric materials recycling can be solved by designing products that will be easy to recycle. In fact, biodegradation is the consistent breaking of chemical links of a polymer molecular chain under the action of microorganisms. Destroying a polymer, bacteria, fungi or algae use the remnants of its molecules as a source of vital organic compounds as well as energy. Usually biodegradation occurs in an aque

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Tarannum, Nazia, Divya Divya, and Yogendra K. Gautam. "Facile green synthesis and applications of silver nanoparticles: a state-of-the-art review." RSC Advances 9, no. 60 (2019): 34926–48. http://dx.doi.org/10.1039/c9ra04164h.

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Pfeiffer, Daniel, and Dieter Jendrossek. "PhaM Is the Physiological Activator of Poly(3-Hydroxybutyrate) (PHB) Synthase (PhaC1) in Ralstonia eutropha." Applied and Environmental Microbiology 80, no. 2 (2013): 555–63. http://dx.doi.org/10.1128/aem.02935-13.

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ABSTRACTPoly(3-hydroxybutyrate) (PHB) synthase (PhaC1) is the key enzyme of PHB synthesis inRalstonia eutrophaand other PHB-accumulating bacteria and catalyzes the polymerization of 3-hydroxybutyryl-CoA to PHB. Activity assays ofR. eutrophaPHB synthase are characterized by the presence of lag phases and by low specific activity. It is assumed that the lag phase is caused by the time necessary to convert the inactive PhaC1 monomer into the active dimeric form by an unknown priming process. The lag phase can be reduced by addition of nonionic detergents such as hecameg [6-O-(N-heptyl-carbamoyl)-

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York, Gregory M., Björn H. Junker, JoAnne Stubbe, and Anthony J. Sinskey. "Accumulation of the PhaP Phasin of Ralstonia eutropha Is Dependent on Production of Polyhydroxybutyrate in Cells." Journal of Bacteriology 183, no. 14 (2001): 4217–26. http://dx.doi.org/10.1128/jb.183.14.4217-4226.2001.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are polyoxoesters that are produced by diverse bacteria and that accumulate as intracellular granules. Phasins are granule-associated proteins that accumulate to high levels in strains that are producing PHAs. The accumulation of phasins has been proposed to be dependent on PHA production, a model which is now rigorously tested for the phasin PhaP of Ralstonia eutropha. R. eutropha phaC PHA synthase and phaP phasin gene replacement strains were constructed. The strains were engineered to express heterologous and/or mutant PHA synthase alleles and aphaP-gfp

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Afghan, Inzer Gul, and Anupama Shrivastav. "Isolation and Screening of Polyhydroxyalkanoates (PHA) Producing Bacteria Utilizing Agricultural Waste." International Journal of Applied Sciences and Biotechnology 8, no. 3 (2020): 336–42. http://dx.doi.org/10.3126/ijasbt.v8i3.31566.

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Polyhydroxyalkanoates (PHAs) are biosynthetic, environmentally friendly and biodegradable polyester stored as a granules inside the cytoplasm of microorganisms, granules are compounds of PHAs used as carbon and energy source, Synthetic polymer take many years to demolish completely, microorganisms can degrade PHAs within a year into carbon dioxide, water and energy, The main contributor for PHAs production cost is carbon sources cost, Accordingly it is favorable to produce PHA from any agriculture waste like rice bran. Aim of this dissertation is to utilize rice bran which was obtained from Li

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Touloupakis, Eleftherios, Eleni G. Poloniataki, Martina Casciana, Demetrios F. Ghanotakis та Pietro Carlozzi. "Poly-β-Hydroxybutyrate Production by Rhodopseudomonas sp. Grown in Semi-Continuous Mode in a 4 L Photobioreactor". Symmetry 13, № 9 (2021): 1609. http://dx.doi.org/10.3390/sym13091609.

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The synthesis of polyhydroxybutyrate (PHB) by photosynthetic non-sulfur bacteria is a potential approach for producing biodegradable plastics. In this work, acetate was used as a single carbon source to study the effect on PHB formation in Rhodopseudomonas sp. cultured in a cylindrical four-liter photobioreactor under semi-continuous mode. The cultivation process is divided into a symmetrical growth phase and a PHB accumulation phase separated temporally. The symmetrical growth phase (nutrient sufficient conditions) was followed by a sulfur-limited phase to promote PHB accumulation. The main n

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Bonartsev, A. P., G. A. Bonartseva, I. V. Reshetov, K. V. Shaitan, and M. P. Kirpichnikov. "Application of Polyhydroxyalkanoates in Medicine and the Biological Activity of Natural Poly(3-Hydroxybutyrate)." Acta Naturae 11, no. 2 (2019): 4–16. http://dx.doi.org/10.32607/20758251-2019-11-2-4-16.

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Biodegradable and biocompatible polymers, polyhydroxyalkanoates (PHAs), are actively used in medicine to produce a wide range of medical devices and dosage formulations. The medical industry mainly utilizes PHAs obtained by chemical synthesis, but interest in the medical application of natural PHAs obtained biotechnologically is also growing. Synthetic PHAs are the biomimetic analogs of bacterial poly(3-hydroxybutyrate) (PHB) and other natural PHAs. This paper addresses the issue of the presence of biological activity in synthetic and natural PHAs (stimulation of cell proliferation and differe

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Olubayi, Olubayi, Rodulio Caudales, Amy Atkinson, and Carlos A. Neyra. "Differences in chemical composition between nonflocculated and flocculated Azospirillum brasilense Cd." Canadian Journal of Microbiology 44, no. 4 (1998): 386–90. http://dx.doi.org/10.1139/w98-002.

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A two-step broth replacement method was used to induce Azospirillum brasilense Cd bacteria to flocculate in vitro. Nonflocculated and flocculated cells were compared with regard to total cellular lipid composition, fatty acid profiles, and poly-beta-hydroxybutyrate (PHB), protein, and carbohydrate contents. The fatty acid profiles of nonflocculated and flocculated cells were qualitatively identical. Two unsaturated fatty acids, octadecanoate (18:1 cis-9) and hexadecanoate (16:1 cis-9), accounted for approximately 80% of the total fatty acid content in both phenotypes. The major lipids in nonfl

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Terpe, Kay, Kirsten Kerkhoff, Elena Pluta, and Dieter Jendrossek. "Relationship between Succinate Transport and Production of Extracellular Poly(3-Hydroxybutyrate) Depolymerase inPseudomonas lemoignei." Applied and Environmental Microbiology 65, no. 4 (1999): 1703–9. http://dx.doi.org/10.1128/aem.65.4.1703-1709.1999.

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ABSTRACT The relationship between extracellular poly(3-hydroxybutyrate) (PHB) depolymerase synthesis and the unusual properties of a succinate uptake system was investigated in Pseudomonas lemoignei. Growth on and uptake of succinate were highly pH dependent, with optima at pH 5.6. Above pH 7, growth on and uptake of succinate were strongly reduced with concomitant derepression of PHB depolymerase synthesis. The specific succinate uptake rates were saturable by high concentrations of succinate, and maximal transport rates of 110 nmol/mg of cell protein per min were determined between pH 5.6 an

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de Almeida, Alejandra, Pablo I. Nikel, Andrea M. Giordano, and M. Julia Pettinari. "Effects of Granule-Associated Protein PhaP on Glycerol-Dependent Growth and Polymer Production in Poly(3-Hydroxybutyrate)-Producing Escherichia coli." Applied and Environmental Microbiology 73, no. 24 (2007): 7912–16. http://dx.doi.org/10.1128/aem.01900-07.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are accumulated as intracellular granules by many bacteria under unfavorable conditions, enhancing their fitness and stress resistance. Poly(3-hydroxybutyrate) (PHB) is the most widespread and best-known PHA. Apart from the genes that catalyze polymer biosynthesis, natural PHA producers have several genes for proteins involved in granule formation and/or with regulatory functions, such as phasins, that have been shown to affect polymer synthesis. This study evaluates the effect of PhaP, a phasin, on bacterial growth and PHB accumulation from glycerol in bi

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Szkopińska, A. "Ubiquinone. Biosynthesis of quinone ring and its isoprenoid side chain. Intracellular localization." Acta Biochimica Polonica 47, no. 2 (2000): 469–80. http://dx.doi.org/10.18388/abp.2000_4027.

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Ubiquinone, known as coenzyme Q, was shown to be the part of the metabolic pathways by Crane et al. in 1957. Its function as a component of the mitochondrial respiratory chain is well established. However, ubiquinone has recently attracted increasing attention with regard to its function, in the reduced form, as an antioxidant. In ubiquinone synthesis the para-hydroxybenzoate ring (which is the derivative of tyrosine or phenylalanine) is condensed with a hydrophobic polyisoprenoid side chain, whose length varies from 6 to 10 isoprene units depending on the organism. para-Hydroxybenzoate (PHB)

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Maehara, Akira, Shunsaku Ueda, Hideo Nakano, and Tsuneo Yamane. "Analyses of a Polyhydroxyalkanoic Acid Granule-Associated 16-Kilodalton Protein and Its Putative Regulator in the pha Locus of Paracoccus denitrificans." Journal of Bacteriology 181, no. 9 (1999): 2914–21. http://dx.doi.org/10.1128/jb.181.9.2914-2921.1999.

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ABSTRACT The polyhydroxyalkanoic acid (PHA) granule-associated 16-kDa protein (GA16 protein) of Paracoccus denitrificans was identified, and its corresponding gene was cloned and analyzed at the molecular level. The N-terminal amino acid sequence of GA16 protein revealed that its structural gene is located downstream from the PHA synthase gene (phaC Pd) cloned recently (S. Ueda, T. Yabutani, A. Maehara, and T. Yamane, J. Bacteriol. 178:774–779, 1996). Gene walking around phaC Pdrevealed two new open reading frames (ORFs) possibly related to PHA synthesis, one of which was the phaP Pd gene, enc

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Tsai, C. S., and W. T. Liu. "Phylogenetic and physiological diversity of tetrad-forming organisms in deteriorated biological phosphorus removal systems." Water Science and Technology 46, no. 1-2 (2002): 179–84. http://dx.doi.org/10.2166/wst.2002.0474.

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Polyhydroxyalkanoate (PHA)- and polyphosphate-accumulating traits of different taxonomic tetrad-forming organisms (TFOs) in two anaerobic-aerobic sequential batch reactors (SBRs) were characterized by the simultaneous use of fluorescence in-situ hybridization, PHB stain and DAPI stain. The two SBRs with glucose as the main carbon source were operated under different P:total organic carbon feeding ratios for more than 300 days, but both exhibited no enhanced biological phosphorus removal (EBPR) activity. Microscopic observations on sludge samples taken at various times from those two SBRs revea

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Mothes, Gisela, and Wolfgang Babel. "Methylobacterium rhodesianumMB 126 possesses two stereospecific crotonyl-CoA hydratases." Canadian Journal of Microbiology 41, no. 13 (1995): 68–72. http://dx.doi.org/10.1139/m95-170.

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Two distinct crotonyl-CoA hydratases of Methylobacterium rhodesianum MB 126 were separated by column chromatography on DEAE-Sepharose CL-6B. The two enzymes were further purified by chromatography on red 120 agarose and Mono Q. Enzyme A was specific for L(+)-hydroxybutyryl-CoA. It had an apparent molecular weight of 160 000 with two identical subunits of 43 000 and 34 000. The apparent Kmvalues for L(+)-hydroxybutyryl-CoA and crotonyl-CoA were 83 and 90 μM, respectively. Enzyme B was specific for D(−)-hydroxybutyryl-CoA. It had an apparent molecular weight of 39 000 with identical subunits of

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Slater, Steven, Kathryn L. Houmiel, Minhtien Tran та ін. "Multiple β-Ketothiolases Mediate Poly(β-Hydroxyalkanoate) Copolymer Synthesis in Ralstonia eutropha". Journal of Bacteriology 180, № 8 (1998): 1979–87. http://dx.doi.org/10.1128/jb.180.8.1979-1987.1998.

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ABSTRACT Polyhydroxyalkanoates (PHAs) are a class of carbon and energy storage polymers produced by numerous bacteria in response to environmental limitation. The type of polymer produced depends on the carbon sources available, the flexibility of the organism’s intermediary metabolism, and the substrate specificity of the PHA biosynthetic enzymes. Ralstonia eutropha produces both the homopolymer poly-β-hydroxybutyrate (PHB) and, when provided with the appropriate substrate, the copolymer poly(β-hydroxybutyrate-co-β-hydroxyvalerate) (PHBV). A required step in production of the hydroxyvalerate

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Terpolilli, Jason J., Shyam K. Masakapalli, Ramakrishnan Karunakaran, et al. "Lipogenesis and Redox Balance in Nitrogen-Fixing Pea Bacteroids." Journal of Bacteriology 198, no. 20 (2016): 2864–75. http://dx.doi.org/10.1128/jb.00451-16.

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ABSTRACTWithin legume root nodules, rhizobia differentiate into bacteroids that oxidize host-derived dicarboxylic acids, which is assumed to occur via the tricarboxylic acid (TCA) cycle to generate NAD(P)H for reduction of N2. Metabolic flux analysis of laboratory-grownRhizobium leguminosarumshowed that the flux from [13C]succinate was consistent with respiration of an obligate aerobe growing on a TCA cycle intermediate as the sole carbon source. However, the instability of fragile pea bacteroids prevented their steady-state labeling under N2-fixing conditions. Therefore, comparative metabolom

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Lu, Haiwei, Guoliang Yuan, Steven H. Strauss, et al. "Reconfiguring Plant Metabolism for Biodegradable Plastic Production." BioDesign Research 2020 (August 5, 2020): 1–13. http://dx.doi.org/10.34133/2020/9078303.

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For decades, plants have been the subject of genetic engineering to synthesize novel, value-added compounds. Polyhydroxyalkanoates (PHAs), a large class of biodegradable biopolymers naturally synthesized in eubacteria, are among the novel products that have been introduced to make use of plant acetyl-CoA metabolic pathways. It was hoped that renewable PHA production would help address environmental issues associated with the accumulation of nondegradable plastic wastes. However, after three decades of effort synthesizing PHAs, and in particular the simplest form polyhydroxybutyrate (PHB), and

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Raberg, Matthias, Jan Bechmann, Ulrike Brandt, et al. "Versatile Metabolic Adaptations ofRalstonia eutrophaH16 to a Loss of PdhL, the E3 Component of the Pyruvate Dehydrogenase Complex." Applied and Environmental Microbiology 77, no. 7 (2011): 2254–63. http://dx.doi.org/10.1128/aem.02360-10.

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ABSTRACTA previous study reported that the Tn5-induced poly(3-hydroxybutyric acid) (PHB)-leaky mutantRalstonia eutrophaH1482 showed a reduced PHB synthesis rate and significantly lower dihydrolipoamide dehydrogenase (DHLDH) activity than the wild-typeR. eutrophaH16 but similar growth behavior. Insertion of Tn5was localized in thepdhLgene encoding the DHLDH (E3 component) of the pyruvate dehydrogenase complex (PDHC). Taking advantage of the available genome sequence ofR. eutrophaH16, observations were verified and further detailed analyses and experiments were done.In silicogenome analysis reve

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Basta, Altaf H., Vivian F. Lotfy, Khaled Mahmoud, and Nayera A. M. Abdelwahed. "Synthesis and evaluation of protein-based biopolymer in production of silver nanoparticles as bioactive compound versus carbohydrates-based biopolymers." Royal Society Open Science 7, no. 10 (2020): 200928. http://dx.doi.org/10.1098/rsos.200928.

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This overall process deals with evaluating the performance of silver nanoparticles, synthesized from sodium caseinate (SC) as green biological active agent, in comparison with widely produced from carboxymethyl cellulose, other carbohydrates (oxidized nanocellulose fibres (OC) and starch (St)). The TGA, FTIR and TEM, as well as its antimicrobial activities toward pathogenic Gram-positive and Gram-negative bacteria in addition to the yeast strain Candida albicans NRRL Y-477 were examined. In addition, with regard to their anti-tumour activity, the evaluation was studied via many cancer cell lin

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Nagarajan, Dillirani, Ganies Riza Aristya, Yu-Ju Lin, Jui-Jen Chang, Hong-Wei Yen, and Jo-Shu Chang. "Microbial cell factories for the production of polyhydroxyalkanoates." Essays in Biochemistry 65, no. 2 (2021): 337–53. http://dx.doi.org/10.1042/ebc20200142.

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Abstract Pollution caused by persistent petro-plastics is the most pressing problem currently, with 8 million tons of plastic waste dumped annually in the oceans. Plastic waste management is not systematized in many countries, because it is laborious and expensive with secondary pollution hazards. Bioplastics, synthesized by microorganisms, are viable alternatives to petrochemical-based thermoplastics due to their biodegradable nature. Polyhydroxyalkanoates (PHAs) are a structurally and functionally diverse group of storage polymers synthesized by many microorganisms, including bacteria and Ar

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del Carmen Doria-Serrano, Ma, S. González-Martínez, and M. Hernández-Esparza. "Biochemical Models for Phosphate Accumulating Microorganisms." Water Science and Technology 26, no. 9-11 (1992): 2245–48. http://dx.doi.org/10.2166/wst.1992.0707.

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Although biochemical models for the Pi accumulation of Acinetobacter have been discussed in the literature, attention should be given to the fact that a wastewater treatment plant implies a mixed population of microorganisms and acetate and other low molecular weight fatty acids are not the only nor the most abundant carbon source available. Other microorganisms known to be present in activated sludge plants are able to accumulate polyP, PHB and/or carbohydrates. These bacteria (Pseudomonas, Aeromonas, Moraxella, Klebsiella, and others) may use acetate, but also glucose and other monosaccharid

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Balalakshmi, Chinnasamy, Naiyf S. Alharbi, Shine Kadaikunnan, et al. "Development of chitosan/agar-silver nanoparticles-coated paper for antibacterial application." Green Processing and Synthesis 9, no. 1 (2020): 751–59. http://dx.doi.org/10.1515/gps-2020-0070.

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AbstractThe radical proliferation of pathogenic bacteria and their infections causes significant issues for human health and the environment. Today, biopolymers are used to produce different nanoparticles. In the present investigation, the fabricated chitosan/agar-silver nanoparticles (Cht/Ar-AgNPs)-coated papers were tested for antibacterial applications. Agar was used as a reducing agent for the synthesis of AgNPs. Synthesized Ar-AgNPs were examined through optical, phase crystallinity and topological analysis. Cht and Ar-AgNPs solution was mixed with various ratios of 9:1, 8:2, 7:3, 6:4, an

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Meleshko, А. A., A. G. Afinogenova, G. E. Afinogenov, A. A. Spiridonova та V. P. Tolstoy. "Аntibacterial inorganic agents: efficiency of using multicomponent systems". Russian Journal of Infection and Immunity 10, № 4 (2020): 639–54. http://dx.doi.org/10.15789/2220-7619-aia-1512.

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Metal and metal oxide nanoparticles (NPs) are promising antibacterial agents. They have a broad antimicrobial activity against both Gram-positive and Gram-negative bacteria, viruses, and protozoans. The use of NPs reduces the possibility of the microbial resistance development. This review briefly shows the general mechanisms and the main factors of antibacterial activity of NPs. In this article, a comprehensive review of the recent researches in the field of new antimicrobial agents with superior long-term bactericidal activity and low toxicity is provided. The review gives the examples of sy

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Wübbeler, Jan Hendrik, Nadine Bruland, Milena Wozniczka, and Alexander Steinbüchel. "Biodegradation of the xenobiotic organic disulphide 4,4′-dithiodibutyric acid by Rhodococcus erythropolis strain MI2 and comparison with the microbial utilization of 3,3′-dithiodipropionic acid and 3,3′-thiodipropionic acid." Microbiology 156, no. 4 (2010): 1221–33. http://dx.doi.org/10.1099/mic.0.036178-0.

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Application of the non-toxic 3,3′-thiodipropionic acid (TDP) and 3,3′-dithiodipropionic acid (DTDP) as precursors for the microbial production of polythioesters (PTEs), a class of biologically persistent biopolymers containing sulphur in the backbone, was successfully established previously. However, synthesis of PTEs containing 4-mercaptobutyrate (4MB) as building blocks could not be achieved. The very harmful 4MB is not used as a PTE precursor or as the carbon source for growth by any known strain. As a promising alternative, the harmless oxidized disulfide of two molecules of 4MB, 4,4′-dith

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Turanska, S. P., А. L. Petranovska, V. V. Turov, and P. P. Gorbyk. "Lectins: obtaining, properties, application in biology and medicine." Surface 12(27) (December 30, 2020): 289–326. http://dx.doi.org/10.15407/surface.2020.12.289.

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The review material belongs to the scientific and practical issues related to the interdisciplinary direction on the border of nanotechnology, surface chemistry and physics, biology and medicine and is based on the use of natural components in the composition of iron-containing bioactive nanocomposites and magnetic fluids in creating effective vector systems for antitumor therapy with minimized side effects on the human body and improved compatibility with other drugs. Such natural components, which have unique properties, significant and not yet realized potential opportunities for practical

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Müller-Santos, Marcelo, Janne J. Koskimäki, Luis Paulo Silveira Alves, Emanuel Maltempi de Souza, Dieter Jendrossek, and Anna Maria Pirttilä. "The protective role of PHB and its degradation products against stress situations in bacteria." FEMS Microbiology Reviews, October 28, 2020. http://dx.doi.org/10.1093/femsre/fuaa058.

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ABSTRACT Many bacteria produce storage biopolymers that are mobilized under conditions of metabolic adaptation, for example, low nutrient availability and cellular stress. Polyhydroxyalkanoates are often found as carbon storage in Bacteria or Archaea, and of these polyhydroxybutyrate (PHB) is the most frequently occurring PHA type. Bacteria usually produce PHB upon availability of a carbon source and limitation of another essential nutrient. Therefore, it is widely believed that the function of PHB is to serve as a mobilizable carbon repository when bacteria face carbon limitation, supporting

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P S, Vinod, Neha Guttikonda, Snehal M. Mathe, and M. B. Sulochana. "Insilico Analysis of PHB from Halophiles for potential Bio-medical applications." International Journal of Scientific Research in Science and Technology, May 16, 2019, 211–18. http://dx.doi.org/10.32628/ijsrst196338.

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Polyhydroxybutyrate (PHB) is an important biopolymer accumulated by halophilic organisms. PHA is a family of polyesters is accumulated as granules in the cell of bacteria. Polyhydroxybutyrate (PHB) can be used as an alternative polymer to polylactide-glycolides for drug carrier production. It is a linear homopolymer biosynthesized by various strains of bacteria by condensation of D (-)-B-hydroxybutyric acid and used as an energy and carbon source. PHB can be obtained by extraction from bacteria or by chemical synthesis. To be suitable as drug carrier the PHB (polymer) has to be biocompatible,

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Cardozo, Javier Ricardo Gómez, Rodrigo Velasco Buscheli, Carlos del Cerro Sánchez, Isabel de la Mata Riesco, and Amanda Lucía Mora Martínez. "Engineering of Bacillus megaterium for improving PHA production from glycerol." Asia Pacific Journal of Molecular Biology and Biotechnology, August 1, 2019, 64–72. http://dx.doi.org/10.35118/apjmbb.2019.027.3.07.

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There are a few PHA-producer bacteria that can uptake glycerol to produce this biopolymer. Among them, Bacillus megaterium LVN01 has demonstrated to be able to grow up using glycerol as a carbon source. Glycerol dehydrogenase (GD) plays a key role in the synthesis of PHA from glycerol. In this study, the improvement of glycerol uptake by a recombinant strain of B. megaterium carrying pHT01-bmgd was evaluated in order to enhance PHA production. The biomass and PHA production were evaluated and compared to wild-type. It was determined that the PHA produced by both strains was PHB and the highest

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Pagliano, Giorgia, Wanda Gugliucci, Elena Torrieri, et al. "Polyhydroxyalkanoates (PHAs) from dairy wastewater effluent: bacterial accumulation, structural characterization and physical properties." Chemical and Biological Technologies in Agriculture 7, no. 1 (2020). http://dx.doi.org/10.1186/s40538-020-00197-1.

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Abstract Background To establish bioplastics as a real alternative to conventional plastics, high production costs must be constrained by using different kinds of wastewater streams as organic substrates and novel microbial strains as material-accumulating bacteria with high performance. Volatile fatty acids (VFAs) from the effluent of dairy wastewater biodigestion represent a new and inexpensive feedstock, which was used in this study for biopolymer production through microbial processes. Results Cupriavidus necator DSM 13513 was particularly able to accumulate PHAs when operating in fed-batc

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