Academic literature on the topic 'Bioscreen C system'

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Journal articles on the topic "Bioscreen C system"

1

Rossi-Rodrigues, Bianca Caroline, Márcia Regina Brochetto-Braga, Sâmia Maria Tauk-Tornisielo, Eleonora Cano Carmona, Valeska Marques Arruda, and José Chaud Netto. "Comparative growth of trichoderma strains in different nutritional sources, using bioscreen c automated system." Brazilian Journal of Microbiology 40, no. 2 (2009): 404–10. http://dx.doi.org/10.1590/s1517-83822009000200035.

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2

Witkowska, Danuta, Katarzyna Buska-Pisarek, Wojciech Łaba, Michał Piegza, and Anna Kancelista. "Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products." Polish Journal of Microbiology 66, no. 2 (2017): 181–88. http://dx.doi.org/10.5604/01.3001.0010.4361.

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Growth kinetics of four Trichoderma strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media. Although the process of lyophilization reduced the survival rate by 50%-60%, the actual number of viable cells in obtained biopreparations remained relatively high (0.58 × 108 - 1.68 × 108 CFU/g dm). The studied strains in the preserved biopreparations were characterized by a high growth rate, as evaluated in microcultures using the Bioscreen C system.
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3

Tauk-Tornisiel, S. M., M. C. Vallejo, and J. C. Govone. "BIOMASSES AND XYLANASE PRODUCTION BY STRAINS OF PENICILLIUM ISOLATED FROM BRAZILIAN ATLANTIC FOREST." Arquivos do Instituto Biológico 76, no. 3 (2009): 359–64. http://dx.doi.org/10.1590/1808-1657v76p3592009.

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ABSTRACT Six Penicillium strains were isolated from soil at a depth of 0 15 cm in the Juréia-Itatins Ecology Station (JIES), in the São Paulo State, Brazil. They were evaluated for xylanase production under different temperatures and carbon sources. The best carbon source and temperature were first determined in an automated Bioscreen C system, verifying the growth of microorganisms. Liquid media containing tap water with 2% carbohydrate and/or 1% nitrogen sources were used. Afterwards, Penicillium citrinum, P. fellutanum, P. rugulosum and P. decumbens were cultivated in 250 mL Erlenmeyer flasks with 50 mL of culture medium containing tap water sole 2% carbon source (fructose, glucose, mannitol, sucrose or xylose) and 1% yeast extract as a nitrogen source at pH 5.0 and 28o C, with agitation of 150 rpm for 72 hours. These same strains, except P. decumbens, and P. purpurogenum were cultivated in solid substrate with wheat bran under the same environmental conditions to study the potential of xylanase activity. Maximum xylanase activity was observed in cultures with wheat bran, without the addition of any other carbon source, using inocula containing 1 x 107 spores.mL-1 (28o C, pH 5.0, 72 h). It can be concluded that P. fellutanum and P. citrinumare a good xylanase producers under the conditions of 28º C. The results of xylanase activity were 54% less at 28º C in liquid cultures media cultures than in solid substrate.
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4

Maina, Gitahi S., Juma K. Kelvin, Mwangi B. Maina, et al. "Antinociceptive properties of dichloromethane: methanolic leaf and root bark extracts of Carissa edulis in rats." Journal of Phytopharmacology 4, no. 2 (2015): 106–12. http://dx.doi.org/10.31254/phyto.2015.4209.

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Conventional medications are expensive and arguably associated with various severe adverse effects, hence the need to develop herbal agents that are effective as alternative. Carissa edulis (Forssk.) Vahl is the herb that has previously made thousands of people flock to a remote Loliondo village in Northern Tanzania, for its cure said to apply to all diseases such as cancer, HIV/AIDS, ulcers, hypertension, and diabetes. Although C. edulis (Forssk.)Vahl is widely used for pain in the traditional system of medicine; review of the literature shows no scientifically investigated report of its described effects. This study was, therefore, designed to bioscreen the DCM: methanolic extract of the leaf and root bark of C. edulis on anti-nociceptive potential. The plant parts were collected from Siakago-Mbeere north sub-county, Embu County, Kenya. Pain was induced into the rats experimentally using formalin. Anti-nociceptive activities in rats were compared with diclofenac (15 mg/kg) as the standard conventional drug. The leaf extract reduced pain by between 47.04% - 47.19% (in the early phase) and 38.96% - 89.26% (in the late phase) while the root bark extracts reduced it by between 21.5% - 41.89% (in the early phase) and between 21.4% - 90.62% (in the later phase). Diclofenac reduced pain by between 27.37% - 34.9% (in the early phase) and 88.24% - 90.28% (in the late phase). Further, the phytochemical screening results showed that the extract had alkaloids, flavonoids, steroids, saponins, phenolics and terpenoids which have been associated with anti-nociceptive activities. Therefore, the study has established that the DCM: methanolic extracts of C. edulis (Forssk.)Vahl are effective in the management of pain.
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5

Mikes, J., M. Siglova, A. Cejkova, J. Masak, and V. Jirku. "The influence of heavy metals on the production of extracellular polymer substances in the processes of heavy metal ions elimination." Water Science and Technology 52, no. 10-11 (2005): 151–56. http://dx.doi.org/10.2166/wst.2005.0689.

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Wastewaters from a chemical industry polluted by heavy metal ions represent a hazard for all living organisms. It can mean danger for ecosystems and human health. New methods are sought alternative to traditional chemical and physical processes. Active elimination process of heavy metals ions provided by living cells, their components and extracellular products represents a potential way of separating toxic heavy metals from industrial wastewaters. While the abilities of bacteria to remove metal ions in solution are extensively used, fungi have been recognized as a promising kind of low-cost adsorbents for removal of heavy-metal ions from aqueous waste sources. Yeasts and fungi differ from each other in their constitution and in their abilities to produce variety of extracellular polymeric substances (EPS) with different mechanisms of metal interactions. The accumulation of Cd(2+), Cr(6+), Pb(2+), Ni(2+) and Zn(2+) by yeasts and their EPS was screened at twelve different yeast species in microcultivation system Bioscreen C and in the shaking Erlenmayer's flasks. This results were compared with the production of yeast EPS and the composition of yeast cell walls. The EPS production was measured during the yeast growth and cell wall composition was studied during the cultivations in the shaking flasks. At the end of the process extracellular polymers and their chemical composition were isolated and amount of bound heavy metals was characterized. The variable composition and the amount of the EPS were found at various yeast strains. It was influenced by various compositions of growth medium and also by various concentrations of heavy metals. It is evident, that the amount of bound heavy metals was different. The work reviews the possibilities of usage of various yeast EPS and components of cell walls in the elimination processes of heavy metal ions. Further the structure and properties of yeasts cell wall and EPS were discussed. The finding of mechanisms mentioned above is necessary to identify the functional groups entered in the metals elimination processes.
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6

WITKOWSKA, DANUTA, KATARZYNA BUSKA-PISAREK, WOJCIECH ŁABA, MICHAŁ PIEGZA, and ANNA KANCELISTA. "Effect of Lyophilization on Survivability and Growth Kinetic of Trichoderma Strains Preserved on Various Agriculture By-Products." Polish Journal of Microbiology 66, no. 2 (2017). https://doi.org/10.5604/01.3001.0010.4361.

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Growth kinetics of four Trichoderma strains was tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media. Although the process of lyophilization reduced the survival rate by 50–60%, the actual number of viable cells in obtained biopreparations remained relatively high (0.58 × 108 – 1.68 × 108 CFU/g dm). The studied strains in the preserved biopreparations were characterized by a high growth rate, as evaluated in microcultures using the Bioscreen C system.
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7

Garcia, Marcelo Valle, Esther Garcia-Cela, Naresh Magan, Marina Venturini Copetti, and Angel Medina. "Comparative Growth Inhibition of Bread Spoilage Fungi by Different Preservative Concentrations Using a Rapid Turbidimetric Assay System." Frontiers in Microbiology 12 (June 8, 2021). http://dx.doi.org/10.3389/fmicb.2021.678406.

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Bread and intermediate moisture bakery products are mainly spoiled by yeasts and filamentous fungi. The inoculum load and preservation system used determines their shelf life. To extend the shelf life of such commodities, the use of chemical preservatives is the most common way to try and control the initiation of mold spoilage of bread. This study has utilized a rapid turbidimetric assay system (Bioscreen C) to examine the temporal efficacy of calcium propionate (CP) and potassium sorbate (PS) for controlling the growth of important bread spoilage fungi. The objectives were to compare the temporal growth of strains of three important spoilage fungi Hyphopichia burtonii (HB17), Paecilomyces variotii (PV11), and Penicillium roqueforti (PR06) isolated from visibly molded bread to (a) different concentrations of CP and PS (0–128 mM), (b) temperatures (25°C, 30°C), (c) water activity (aw; 0.95, 0.97), and (d) pH (5.0, 5.5). All three abiotic factors, pH, aw, and temperature, and preservative concentrations influenced the relative growth of the species examined. In general, PS was more effective than CP in inhibiting the growth of the strains of these three species. In addition, the Time to Detection (TTD) for the efficacy of the preservatives under the interacting abiotic factors was compared. The strain of Paecilomyces variotii (PV10) was the most tolerant to the preservatives, with the shortest TTD values for both preservatives. P. roqueforti was the most sensitive with the longest TTD values under all conditions examined. These results are discussed in the context of the evolution of resistance to food-grade preservatives by such spoilage fungi in bakery products.
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8

Hirvonen, Johanna, Krista Salli, Heli Putaala, Kirsti Tiihonen, Johanna Maukonen, and Arthur Ouwehand. "Selective Utilization of Human Milk Oligosaccharides 2’-FL and 3-FL by Probiotic Bacteria Resulting in Different Metabolite Production by These Bacteria (P20-012-19)." Current Developments in Nutrition 3, Supplement_1 (2019). http://dx.doi.org/10.1093/cdn/nzz040.p20-012-19.

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Abstract Objectives Human breast milk contains a high quantity of oligosaccharides (HMOs) which are not digested by infants but are selectively utilized by beneficial gut microbes such as Bifidobacterium species. The gut microbiome in breast-fed infants is dominated by Bifidobacterium species, whereas formula-fed infants have a higher representation of Bacteroides and Enterobacteria species. The aim of this study was to evaluate the ability of various potentially pathogenic bacteria and commercially available probiotic strains to grow using the HMOs 2’-fucosyllactose (2’-FL) and 3-fucosyllactose (3-FL), glucose, lactose or galacto-oligosaccharide (GOS) as a sole carbon source. We also compared the production of short-chain fatty acids (SCFAs) and lactic acid during the fermentation of 2’-FL by B. infantis, B. bifidum and Bacteroides strains. Methods Bacterial growth was monitored by measuring the absorbance at 600 nm every 30 min for 24 h using the automatic Bioscreen© C system under anaerobic conditions in culture media containing 1% 2’-FL, 3-FL, glucose, lactose or GOS as a sole carbon source. Metabolites were analyzed by chromatographic methods in spent culture media after over-night growth of bacterial strains with 1% 2’-FL. Results Only certain bifidobacteria strains, B. infantis and B. bifidum, were able to utilize 2’-FL and 3-FL, whereas other tested Lactobacillus and Bifidobacterium strains as well as pathogenic bacteria grew with all other carbon sources. We also confirmed that Bacteroides fragilis, B. thetaiotaomicron and B. vulgatus strains utilized HMO. Distinct SCFA and lactic acid quantities were observed between Bifidobacterium and Bacteroides strains in 2’-FL fermentation. For instance, acetic and lactic acids were produced at much higher levels in Bifidobacterium than in Bacteroides, and propionic acid was produced at low level only in Bacteroides. Conclusions These results show the selectivity of 2’-FL and 3-FL utilization in certain Bifidobacterium and Bacteroides strains, which is translated to differences in metabolite production between these individual HMO-utilizing bacteria. Further studies will enlighten the role and potential benefits of HMOs as a selective substrate for gut microbes, as well as for competition and cross-feeding of microbial metabolites among gut species. Funding Sources This research was funded by DuPont Nutrition and Health.
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