Academic literature on the topic 'Biosurfactant'
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Journal articles on the topic "Biosurfactant"
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Brinda, C. M., R. Ragunathan R. Ragunathan, and Jesteena Johney. "Diversity and Distribution of Potential Biosurfactant Producing Bacillus Sp MN 243657, GC-MS Analysis and its Antimicrobial Study." Biosciences Biotechnology Research Asia 20, no. 1 (March 30, 2023): 271–91. http://dx.doi.org/10.13005/bbra/3088.
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Biosurfactants are microbially produced surface-active compounds. They are amphiphilic molecules with hydrophilic and hydrophobic regions. The demand for biosurfactants has been exponentially growing as they are nontoxic and biodegradable. They have different applications in several industrial sectors. The objective of this study was to isolate and characterize the native bacteria which produce biosurfactants from oil contaminated soil of different places in Kerala and Tamil Nadu, India. The soil samples were collected from petrol pumps and workshops where the soil is contaminated with petrol, diesel and oil. The bacteria were isolated from contaminated soil samples and confirmed as Bacillus sp.The cultures were screened for biosurfactant production by different screening techniques such as blood hemolysis, oil spreading assay, emulsification ability assay, bacterial adherence to hydrocarbons activity. The potential biosurfactant producing culture was selected and identified using molecular techniques and submitted to NCBI Gene Bank (MN 243657 – Bacillus sp). The selected bacterial culture was used for biosurfactant production and these were characterized by UV, TLC, FTIR and GC -MS analysis. The derived biosurfactant's Rf value was 0.68 as determined by a TLC chromatogram. In a UV-visible spectroscopy study, the isolated biosurfactant displayed a highest peak at 415 nm. According to FTIR analysis, the isolated biosurfactant displayed an intense peak at 3340 cm -1. The large peaks of the biosurfactant were observed at various retention times of 12.75, 10.22, 4.98, and 3.87, respectively, after GC-MS analysis. Antibacterial and antifungal activity of the biosurfactant was identified against pathogenic bacteria such as P.aeruginosa, E. coli, K. pneumoniae, S. aureus and fungi Aspergillus niger, Aspergillus terreus, Aspergillus flavus.
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Liepins, Janis, Karina Balina, Raimonda Soloha, Ieva Berzina, Liva Kristiana Lukasa, and Elina Dace. "Glycolipid Biosurfactant Production from Waste Cooking Oils by Yeast: Review of Substrates, Producers and Products." Fermentation 7, no. 3 (July 29, 2021): 136. http://dx.doi.org/10.3390/fermentation7030136.
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Biosurfactants are a microbially synthesized alternative to synthetic surfactants, one of the most important bulk chemicals. Some yeast species are proven to be exceptional biosurfactant producers, while others are emerging producers. A set of factors affects the type, amount, and properties of the biosurfactant produced, as well as the environmental impact and costs of biosurfactant’s production. Exploring waste cooking oil as a substrate for biosurfactants’ production serves as an effective cost-cutting strategy, yet it has some limitations. This review explores the existing knowledge on utilizing waste cooking oil as a feedstock to produce glycolipid biosurfactants by yeast. The review focuses specifically on the differences created by using raw cooking oil or waste cooking oil as the substrate on the ability of various yeast species to synthesize sophorolipids, rhamnolipids, mannosylerythritol lipids, and other glycolipids and the substrate’s impact on the composition, properties, and limitations in the application of biosurfactants.
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Oyedeji, Olaoluwa, Deborah Ifeoluwa Onifade, and Anthony Abiodun Onilude. "Production, Characterization, and Application of Biosurfactant From Lactobacillus plantarum OG8 Isolated From Fermenting Maize (Zea Mays) Slurry." Acta Universitatis Cibiniensis. Series E: Food Technology 26, no. 2 (December 1, 2022): 271–86. http://dx.doi.org/10.2478/aucft-2022-0022.
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Abstract Biosurfactants have wide applications in several industries. However, high production costs and safety concerns have limited their comprehensive use. Twenty-five strains of lactic acid bacteria, isolated from fermenting maize slurry, were screened for biosurfactant production using the emulsification activity (E24) assay. The selected bacterium was identified molecularly using the 16S rRNA gene sequencing as Lactobacillus plantarum OG8. The effect of some cultural factors on biosurfactant production from the bacterium, using pineapple peel as a low-cost substrate, was investigated. The optimum yield of biosurfactant occurred at a 48 h incubation period, using glucose and peptone as carbon and nitrogen sources, respectively. The biosurfactant was characterized to possess mostly carbohydrates, followed by protein and lipid contents. Optima pH 10.0 and temperature 60 °C were the best for the biosurfactant activity. The biosurfactant exhibited antimicrobial activity against bacterial pathogens Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae, at a concentration of 5.0 mg/mL. The use of pineapple peel as a low-cost substrate for biosurfactant production from Lactobacillus plantarum OG8 will serve for cost-effective production. The biosurfactantt produced exhibited promising properties such as thermostability and antimicrobial activity against food spoilage and pathogenes that could make it suitable for food processing and preservation.
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Amraini, Said Zul, Sri Rezeki Muria, Bahruddin Bahruddin, Irdoni HS, Ulfa Dwi Artha, and Reno Susanto. "Biosurfactant Production from Pseudomonas aeruginosa ATCC27853 with Carbon Source from Crude Palm Oil for Oil Recovery." Indo. J. Chem. Res. 10, no. 1 (May 31, 2022): 47–52. http://dx.doi.org/10.30598/ijcr.2022.10-sai.
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Biosurfactants are surfactants that are synthesized by microorganisms using organic materials and have biodegradable properties, making them environmentally friendly. One of the applications of biosurfactants in the recovery of petroleum. This study aims to determine the type of biosurfactant produced by Pseudomonas aeruginosa bacteria using crude palm oil as the main carbon source, to determine the effect of variations in pH and CPO concentration on surface tension reduction and emulsification, and compare the best biosurfactant with surfactant synthesis. The production of biosurfactants has 3 stages, namely the bacterial preparation, the biosurfactant production, and the analysis in the form of surface tension, emulsification, crude oil removal, and FTIR. The best biosurfactant was obtained at pH 7 and a carbon source concentration of 3% v/v with surface tension and emulsification values of 42.49 mN/m and 58%, respectively. The pH value and CPO concentration can affect the growth in the biosurfactant production process, thus also affecting the surface tension and emulsification values. The biosurfactants obtained were rhamnolipid biosurfactants. The biosurfactants produced in this study have lower crude oil recovery capabilities than synthetic surfactants with crude oil removal values of 57.78% and 79.34%, respectively.
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Gunjal, Aparna. "Biosurfactants from renewable sources - A review." Nepal Journal of Environmental Science 10, no. 2 (December 31, 2022): 15–23. http://dx.doi.org/10.3126/njes.v10i2.48538.
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Biosurfactants have wide applications in pharmaceutical, agriculture and food industries. The research area of biosurfactants is gaining immense attention. The review mentions here the advantages and various substrates used for biosurfactants production. The pre-treatment of substrates for biosurfactants production is also focused. The production of biosurfactants by solid state fermentation is also described. The renewable substrates, yield and microorganisms used for biosurfactant production are also taken into consideration. The screening methods for biosurfactant are also described. The use of renewable sources for biosurfactant production is specially focused in the review. This will be very eco-friendly, easy and economical. More studies need to BE done on large-scale production of biosurfactants using genetically engineered microorganisms.
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Youssef, Noha H., Kathleen E. Duncan, and Michael J. McInerney. "Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity." Applied and Environmental Microbiology 71, no. 12 (December 2005): 7690–95. http://dx.doi.org/10.1128/aem.71.12.7690-7695.2005.
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ABSTRACT Biosurfactant production may be an economic approach to improving oil recovery. To obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus Bacillus, were tested for growth and biosurfactant production in medium with 5% NaCl under aerobic and anaerobic conditions. All strains grew aerobically with 5% NaCl, and 147 strains produced a biosurfactant. Thirty-five strains grew anaerobically with 5% NaCl, and two produced a biosurfactant. In order to relate structural differences to activity, eight lipopeptide biosurfactants with different specific activities produced by various Bacillus species were purified by a new protocol. The amino acid compositions of the eight lipopeptides were the same (Glu/Gln:Asp/Asn:Val:Leu, 1:1:1:4), but the fatty acid compositions differed. Multiple regression analysis showed that the specific biosurfactant activity depended on the ratios of both iso to normal even-numbered fatty acids and anteiso to iso odd-numbered fatty acids. A multiple regression model accurately predicted the specific biosurfactant activities of four newly purified biosurfactants (r 2 = 0.91). The fatty acid composition of the biosurfactant produced by Bacillus subtilis subsp. subtilis strain T89-42 was altered by the addition of branched-chain amino acids to the growth medium. The specific activities of biosurfactants produced in cultures with different amino acid additions were accurately predicted by the multiple regression model derived from the fatty acid compositions (r 2 = 0.95). Our work shows that many strains of Bacillus mojavensis and Bacillus subtilis produce biosurfactants and that the fatty acid composition is important for biosurfactant activity.
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Javadi, Ali, Mohamad Reza Pourmand, Javad Hamedi, Fatemeh Gharebaghi, Zohre Baseri, Razieh Mohammadzadeh, and Seyyed Saeed Eshraghi. "Evaluation of anti-biofilm potential of biosurfactant extracted from Nocardia species." Folia Medica 63, no. 3 (June 30, 2021): 392–99. http://dx.doi.org/10.3897/folmed.63.e54386.
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Introduction: Bacterial natural products such as biosurfactants and surface-active agents are important compounds which exhibit many applications in the fields of medicine. Aim: The aim of the present study was to isolate and identify Nocardia strains with high biosurfactant production and antibiofilm ability. Materials and methods: In the present study, a biosurfactant producing Nocardia species was isolated and identified by a laboratory method. Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The oil spreading test and the surface tension measurements showed that one strain was a biosurfactant producer. The strain with the best surface activity results was selected for further studies and identified by 16S rRNA gene sequencing method. Fourier transform infrared spectroscopy (FTIR) and compositional analysis proved a biosurfactant structure. Results: Oil spreading test and blue agar plate test confirmed biosurfactants and extracellular anionic glycolipids. E24% assay using olive oil revealed strong emulsifying characteristic of the extracted biosurfactant with 100% emulsifying strength. FTIR spectrum indicated the presence of aliphatic hydrocarbon chain (lipid) along with the polysaccharide portion, confirming the glycolipid nature of the biosurfactant. The stability of the biosurfactant produced in different conditions was significant. Increasing concentration of BS significantly inhibited Pseudomonas aeruginosa biofilm. Conclusions: N. coubleae can be a representative of the genus Nocardia for the production of biosurfactants with beneficial physicochemical properties.
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Fachria, Rizqy. "APLIKASI BIOSURFAKTAN Bacillus subtilis ATCC 19659 DENGAN MEDIA PRODUKSI LIMBAH TAHU UNTUK ENHACED OIL RECOVERY." Jurnal Teknologi Lingkungan Lahan Basah 9, no. 2 (August 29, 2021): 101. http://dx.doi.org/10.26418/jtllb.v9i2.48221.
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Biosurfactant as secondary metabolit produced by Bacillus subtilis. It has the ability to emulsify and reduce the surface tension. Biosurfactants produced by B. subtilis is a lipopeptide. Furthermore, biosurfactant can be utilized in microbial enhanced oil recovery (MEOR). In this research, biosurfactant of B. subtilis ATCC 19 659 were evaluated. The production use Nutrient Broth (NB) and soybean liquid waste. Application of biosurfactant in oil recovery showed that biosurfactant of NB recover 2 mL crude oil and biosurfactant of soybean liquid waste medium recover 3.67 mL.
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Sena, Hellen Holanda, Michele Alves Sanches, Diego Fernando Silva Rocha, Walter Oliva Pinto Filho Segundo, Érica Simplício de Souza, and João Vicente Braga de Souza. "Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest." International Journal of Microbiology 2018 (2018): 1–8. http://dx.doi.org/10.1155/2018/5684261.
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Biosurfactants are surface-active compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the Amazon forest. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. In addition, the influences of bioprocess factors (carbon source, nitrogen source, pH, and fermentation time) were investigated. Finally, the biosurfactant produced was semipurified and submitted to stability tests. One hundred fungal cultures were obtained from the soil samples, identified by micromorphology, and submitted to screening for biosurfactant production. Sixty-one strains produced biosurfactants. The strainPenicillium8CC2 showed the highest emulsification index (54.2%). The optimized bioprocess conditions for biosurfactant production byPenicillium8CC2 were as follows: soybean oil, 20 g/L; yeast extract, 30 g/L; pH 9; duration of 9 days. The semipurified biosurfactant showed stability after heating at 100°C for 60 min and after the addition of 30% NaCl (w/v). Tween 80 (0.2% w/v), a conventional surfactant, was used as the control.
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Vaijayanti, Mahulkar Ankita Vidyadhar. "Comparative study of antimicrobial efficiency of biosurfactant producing Pseudomonas spp. from different soil samples." Journal of Applied and Advanced Research 5 (September 6, 2020): 1. http://dx.doi.org/10.21839/jaar.2020.v5.318.
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Amphiphilic biosurfactants are surface-active biological molecules secreted by hydrocarbanoclastic microorganisms. Biosurfactants are eco-friendly, less toxic, biodegradable, and low-cost material, so it has more advantages over chemical surfactants. In this research, Pseudomonas spp., biosurfactant producing microorganisms isolated from different sources of soil samples. IS1, IS2, IS3, IS4 isolates obtained from Garden soil sample; Metal contaminated soil sample; Petroleum contaminated soil sample; Oil contaminated soil sample; respectively. Each isolates identified as Pseudomonas spp. Furthermore, screened for biosurfactant producers. Each isolate showed positive results for the hemolysis test, drop collapse test, oil displacement test, and emulsification test. All isolate incubated in mineral salt medium for biosurfactant production. Biosurfactant extracted from IS1, IS2, IS3, IS4 showed 35%, 65%, 20%, 52% emulsification index respectively. Antimicrobial activity of extracted biosurfactants against pathogenic microorganisms checked by agar cup method. IS2 isolate shows the highest antimicrobial activity among all. All isolate showed a higher zone of inhibition against gram-positive microorganisms than gram-negative microbes. The purpose of this study involves the assessment of the antimicrobial activity of biosurfactant producers from the soil environment.
Dissertations / Theses on the topic "Biosurfactant"
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Chen, Chien-Yen. "Biosurfactant production." Thesis, University of Oxford, 2005. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.419243.
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Domingues, Patrícia Maia. "Isolation of estuarine biosurfactant-producing bacteria." Master's thesis, Universidade de Aveiro, 2011. http://hdl.handle.net/10773/7773.
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Mestrado em BiotecnologiaBioremediation has proven to be an effective strategy in the recuperation of oil contaminated ecosystems. However most bacteria used in this processes, while being able to degrade a wide range of the oil hydrocarbons, have limited action due to the low water solubility of these compounds. Hence, a possible solution for this problem would be the use of biosurfactant-producing bacteria, since the presence of surfactants help improve the hydrocarbons dispersal, solubilization and bioavailability. The objective of this work was to assess the biotechnological potential of Ria de Aveiro estuarine system regarding the presence of hydrocarbonoclastic biosurfactant-producing bacteria and to evaluate different combinations of environmental inocula and carbon sources for the isolation of biosurfactants producing bacteria. Selective cultures (diesel, crude and paraffin) were prepared using inocula from different environmental matrixes: samples from the surface microlayer (SML), bulk estuarine sediments and sediments of the rhizosphere of Halimione portulacoides, a characteristic halophyte from the salt marshes of Ria de Aveiro. During the incubation period, the development of the selective cultures was assessed by quantification of colony forming units (CFU). The highest value of CFU was obtained in the crude-sediment culture, while the lowest value was found with the diesel-rhizosphere combination. The DGGE profiles of the 16s rRNA gene fragments of the total community DNA extracted at the end of the incubation of the selective cultures, show that communities were different in terms of structural diversity. The values of the Shannon-Weaver index of diversity indicate that the higher diversity was achieved in the selective cultures with paraffin as carbon source (2.5231), followed by the crude oil (2.2509), and diesel (1.6726) selective cultures. From the selective cultures, 111 presumably hydrocarbonoclastic isolates were obtained after isolation and purification. Of these, 66 were tested for biosurfactant production by the atomized oil assay, with positive results for 17 isolates (25.8%). The environmental matrix with best results was the SML water and diesel was the most effective carbon source. Having in consideration the high number of isolates obtained from the selective cultures and the percentage of biosurfactant producers, the estuarine system of Ria the Aveiro, and in particular the SML, can be regarded as an interesting seedbank for the prospection of hydrocarbonoclastic and biosurfactants producing bacteria. The SML microhabitat shows particularly high biotechnological potential for the isolation of bacterial strains with interesting properties for application in bioremediation strategies in coastal and estuarine areas.
A biorremediação é tida como uma possível estratégia na recuperação de ecossistemas contaminados com hidrocarbonetos. A aplicação eficaz desta tecnologia é, no entanto, muitas vezes limitada pela natureza hidrofóbica dos contaminantes. O recurso a estirpes bacterianas simultaneamente degradadoras de hidrocarbonetos e produtoras de biossurfactantes apresenta um enorme potencial na reciclagem de compostos hidrofóbicos. Assim, o objectivo deste trabalho consistiu em avaliar o potencial biotecnológico do sistema estuarino da Ria de Aveiro quanto à presença de bactérias hidrocarbonoclásticas produtoras de biossurfactantes e a avaliação de várias combinações de inóculos ambientais e fontes de carbono para a obtenção de isolados bacterianos de interesse. Para tal foram realizadas experiências em meios selectivos (diesel, crude e parafina) a partir de inóculos de diferentes matrizes ambientais: amostras da microcamada superficial (SML), sedimentos estuarinos e rizosfera de bancos de Halimione portulacoides, uma planta halófita dos sapais da Ria de Aveiro. O desenvolvimento da cultura ao longo do período de incubação foi avaliado pela contagem de unidades formadoras de colónias (CFUs). A cultura selectiva com maior teor de bactérias cultiváveis foi a de crude-sedimento e aquela em que a abundância bacteriana foi mais baixa foi a de diesel-rizosfera. A partir da análise dos perfis de DGGE dos fragmentos do gene 16s rRNA do DNA total extraído das culturas selectivas verificou-se que no fim do período de incubação, o grau de semelhança entre as comunidades bacterianas das culturas selectivas é relativamente baixo. Pelo índice de diversidade de Shannon-Weaver a maior diversidade estrutural das comunidades bacterianas encontra-se nas culturas selectivas de parafina (2,5231), seguidas das de crude (2.2509) e das de diesel (1.6727). Das culturas selectivas, foi obtido um conjunto de isolados que foi testado quanto à capacidade de produção de biossurfactantes pelo método atomized oil. De 66 isolados testados, 17 produziram resultado positivo (25,8%), sendo a água da SML a matriz ambiental com melhores resultados e o diesel a melhor fonte de carbono para o isolamento de bactérias produtoras de biossurfactantes. Tendo em conta o elevado número de isolados obtidos e a percentagem de produtores de biossurfactantes, pode concluir-se que na Ria de Aveiro, particularmente na SML, existem comunidades bacterianas adaptadas à utilização se substratos hidrofóbicos, com uma boa representação de produtores de biossurfactantes. Os resultados confirmam a perspectiva de que a SML da Ria de Aveiro é um microhabitat com elevado potencial biotecnológico para isolamento de estirpes de bactérias hidrocarbonoclásticas produtoras de biossurfactantes com promissoras aplicações em processos de biorremediação de regiões estuarinas e costeiras após contaminação acidental com hidrocarbonetos de petróleo.
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Bamara, Prosper. "Conversion of hydrocarbons to biosurfactants : an insight into the bioprocess optimisation of biosurfactant production using alkanes as inducers." Master's thesis, University of Cape Town, 2009. http://hdl.handle.net/11427/5344.
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Surfactants are chemical compounds that are able to alter interfacial properties, particularly surface tension. When they are biologically produced, the term biosurfactant is used. One of the most important groups of biosurfactants is a family of chemical compounds known as glycolipids, whose structure consists of a sugar group and a lipid tail. Glycolipids are subdivided into three main groups: rhamnolipids, sophorolipids and trehalolipids, named following their sugar moieties, respectively rhamnose, trehalose and sophorose. Biosurfactants exhibit attractive advantages over chemical surfactants. Examples of these are biodegradability, low toxicity, and effectiveness at extreme temperature, pH and salinity. The objective of the present research project was, first, to investigate the potential of liquid aliphatic hydrocarbons to induce biosurfactant production by the bacterium Ps. aeruginosa 2Bf isolated based on its ability to metabolise alkanes. The second objective was to optimise biosurfactant production using alkanes as sole carbon and energy source, through optimising the mixing & aeration conditions, media conditions as well as provision of alkane, in a stirred tank batch reactor system. The final objective was to describe the biosurfactant formed. Experiments were organised in three major series: the exploratory shake flask based experiments, the bioreactor-based experiments to optimise biosurfactant production and characterise biokinetics and performance, and the biosurfactant characterisation experiments. Following review of a number of methods, microbial cell counts were selected as the most reproducible measure of biomass formation in the presence of alkanes. The presence of biosurfactant was quantified functionally in terms of the emulsification index and alteration of surface tension. Using a shake flask-based study, nitrogen source was investigated in terms of biomass and biosurfactant synthesis. Four pre-selected nitrogen sources were tested in order to select the best for bioreactor based study. These nitrogen sources consisted of specific combinations of three nitrogen compounds, NH4NO3, NaNO3 and (NH4)2SO4. During the study, long chain liquid n-alkanes were used as sole carbon source and the C/N ratio maintained at the value of 18.6 in mass terms. Results confirmed that both a combination of NO3 ' and NH4+ ions or a nitrogen source composed solely of NH4+ ions were suitable for biomass growth and biosurfactant production. (NH4)SO4 was used as the N-source of choice in the remainder of the study. While the C14-C17 alkanes cut was the carbon source of interest in the study, two pure alkanes, n-C12 and n-C16 were tested and compared to the C14-C17 blend. The C14-C17 fraction, sourced as an industrial byproduct, compared favourably as a carbon source with respect to hexadecane and dodecane. ii Biosurfactant production was not observed in Ps. aeruginosa 2Bf cultures where glucose was the sole carbon source and the bacteria were not previously exposed to linear alkanes. Using a mixed carbon source of glucose and alkane, or on pre-exposure of the bacteria to alkane, biosurfactant production was induced. Induction was optimised where alkane was the sole carbon source over a period of four sub-culture steps. In the quantitative optimisation of biosurfactant production through the bioreactor based study, mixing and aeration were optimised; agitation and aeration proved to be equally important, the first at intermediate rates, the second at lower rates. Their interaction, when maximum biomass was used as the variable for response, was found to be important for agitation rates up to 500 rpm. Beyond this range of agitation speed, the interaction between aeration and agitation became negligible. In the case of Eindex as the variable for response, similar results were obtained with regard to the impact of the interaction between aeration and agitation on the process. It was significant from lower to intermediate agitation rates, and negligible from intermediate to higher rates of agitation. Lower aeration rate was found to enhance the oxygen utilisation rate, while mass transfer was relatively favoured by high aeration rate. Regarding the emulsification power of the product, quantitative tests were carried out on culture suspension, supernatant prepared by centrifugation and supernatant prepared by centrifugation and filtration at 0.22μm pore size filters. Results showed that some emulsification effect was lost through centrifugation and filtration. This loss of emulsification effect was more pronounced in the filtration case, thus showing that some biosurfactant was removed along some other material or substance through sticking on filter paper. Foam control was required, and two mechanical foam breakers were compared to anti-foam reagent. It was experimentally established that mechanical foam breakers are preferable to chemical anti-foam reagents. On comparing the two different mechanical foam breakers, the modified two blade paddle with three slits, FB-2, performed better than the simple two blade paddle foam breaker, FB-1. Further investigations showed that the interaction between type of foam control and agitation rate was negligible throughout the process. The Biosurfactant was characterised at the structural level and the antibiotic potential of Ps. aeruginosa 2Bf's biosurfactant was analysed. In addition to the thin layer chromatography, three different spectroscopic methods (mass, infrared & nuclear magnetic resonance) were used to study the chemical structure of the biosurfactant produced. Up to six rhamnolipid structures were tentatively identified with spectrometric analysis whereas only four to five structures could be detected with thin layer chromatography. Possession of an anti-microbial activity by the rhamnolipids produced was confirmed with the B. subtilis inhibition test.
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Shen, Hsin-Hui. "Neutron reflection study of the biosurfactant surfactin." Thesis, University of Oxford, 2008. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.491968.
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Three different deuterated surfactins were produced from the Bacillus sub/iUs strain, one perdeuterated, one with the four leucines perdeuterated, and one with everything except the four leucines perdeuterated. The neutron reflectivity profiles ofthese three samples in each of null reflecting water and D20 with a seventh profile of the protonated surfactin in D20 were measured under different pH values and ionic concentrations. This combination of different isotopic compositions made it possible to deduce the distribution of each type of iabelled fragment in the surfactin. The consistency of the three null water profiles showed how well defined the deuteration was in the three deuterated samples. The ball-like structure found for the surfactin at surfaces makes it more like a hydrophobic nanoparticle whose solubility in water is only maintained by the double charge. This is probably what makes it so surface active at such low concentrations and what contributes to its formation of very compact surface layers that are much thinner than observed for most conventional amphiphiles. The adsorbed structures of surfactin at all standard solid/liquid interfaces (silica/water, hydrophobic OTS/water, sapphire/water) at different pH conditions have also been investigated by neutron reflection. Surfactin is quite peculiar; it is basically a charged, or partially charged, hydrophobic ball and the general pattern ofadsorption are quite different from normal. anionic surfactants. The overall thickness of the surfactin is about 1.3 nm. The adsorption varies with different substrates and is dominated by hydrophobic and electrostatic interactions. Contrast variation plays an important role in increasing the sensitivity to bilayers and was utilized extensively in the study of the interaction of surfactin with phospholipids on the silicon/silica surface. The d31-DPPC-surfactin bilayer in D20 is a particularly favourable contrast among the four possibilities (h-DPPCID20, d31-DPPCID20, d62DPPC/ CmSi, and d7s-DPPC /CmSi) for the study of this system. It was found that surfactin disrupts and solubilizes phopsholipid bilayers. This happens when the concentration of surfactin in the bilayer is above a certain threshold and when surfactin micelles are present in the bulk solution. The solubilization rate is related to the bilayer coverage. More detailed studies have shown that the surfactin is eXclusively located in the outer leaflet of the bilayer when it starts to penetrate and in this, and some other features, the adsorption into bilayers resembles that of conventional non-ionic/anionic surfactant mixtures.
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Chen, Zixi. "Polyurethane-Based Biosurfactant Mimics as Antibiofilm Agents." University of Akron / OhioLINK, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=akron1619217360880311.
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Jiménez, Peñalver Pedro. "Sophorolipids production by solid-state fermentation: from lab-scale to pilot plant." Doctoral thesis, Universitat Autònoma de Barcelona, 2017. http://hdl.handle.net/10803/458652.
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En aquest treball es proposa una tecnologia alternativa per a la producció de soforolípids (SLs), un tipus de biosurfactant, presentats com a alternativa als surfactants produïts químicament degut a la seva major eficiència i millor perfil mediambiental. En aquest treball s'han dut a terme dues estratègies per a millorar la relació de cost-eficiència dels SLs respecte als surfactants produïts químicament, que és el que determina la seva viabilitat econòmica. Ambdues estratègies estan basades en la producció de SLs mitjançant la fermentació en estat sòlid.
La primera estratègia va consistir en el ús d’un residu de winterització (RW) amb l'objectiu de disminuir el preu dels substrats i, per tant, el cost final de producció dels SLs. Es va utilitzar melassa de sucre com a co-substrat i palla de blat com a suport inert. El procés va ser optimitzat en base al rati de substrats, la velocitat d’aeració i a la mida de l’inòcul a escala de 100-g, obtenint-se un rendiment de 0.261 g per g de substrat al dia 10. El procés optimitzat, va ser escalat satisfactòriament a un bioreactor de llit fix de 40-L, però posteriorment, es van observar problemes associats amb l'eliminació de calor durant l'escalat a un bioreactor de 100-L amb barreja intermitent. L'estructura química i les propietats interfacials de la barreja natural del SLs produït a partir del RW es va estudiar durant una estança al Rensselaer Polytechnic Institute (NY, USA).
La segona estratègia consistí en l'ús de àcid esteàric (C18:0) per a l'obtenció de SLs amb una estructura específica que millori les propietats fisicoquímiques de la barreja natural de SLs i, per tant, la seva eficiència. Es va utilitzar melassa de sucre com a co-substrat i escuma de poliuretà com a suport inert. L'efecte de la densitat de l'escuma de poliuretà i la capacitat de retenció hídrica van ser avaluades i el procés va ser optimitzat en base al rati de substrats e inòcul, obtenint-se un rendiment final de 0.211 g de SLs per g de substrat. Els SLs produïts contenien elevades quantitats de SLs C18:0.
Es van observar correlacions significatives entre el rendiment de SLs i l’oxigen consumit (COA). Això suggereix que el COA pot ser utilitzat com a mesura indirecta de la producció de SLs per a la monitorització en línea de processos de FES.
Aquesta tesi representa el començament d'una nova línia d'investigació centrada en la producció de SLs per FES en el Grup de Investigació en Compostatge (GICOM) del Departament d’Enginyeria Química, Biològica i Ambiental de la Universitat Autònoma de Barcelona.En este trabajo se propone una tecnología alternativa para producir soforolípidos (SLs), un tipo de biosurfactante, presentados como alternativa a los surfactantes producidos químicamente debido a su mayor eficiencia y mejor perfil medioambiental. En este trabajo se han explorado dos estrategias para mejorar la relación coste-eficiencia de los SLs respecto a los surfactantes producidos químicamente, que es lo que determina su viabilidad económica. Ambas estrategias están basadas en la producción de SLs mediante la fermentación en estado sólido (FES) de Starmerella bombicola. La primera estrategia consistió en el uso de un residuo de winterización (RW) con el fin de disminuir el precio de los sustratos. Se utilizó melaza de azúcar como co-sustrato y paja de trigo como soporte inerte. El proceso fue optimizado en base a la ratio de sustratos, la velocidad de aireación y el tamaño del inóculo a escala de 100-g obteniendo un rendimiento de 0.261 g de SLs por g de sustrato a día 10. El proceso fue escalado satisfactoriamente a un biorreactor de lecho fijo de 40-L, pero se observaron problemas asociados con la eliminación del calor durante el escalado a un biorreactor de 100-L. Los SLs producidos a partir del RW fueron caracterizados durante una estancia en el Rensselaer Polytechnic Institute (RPI) en NY, EEUU. La segunda estrategia consistió en el uso de ácido esteárico (C18:0) para obtener SLs con una estructura específica que mejore las propiedades fisicoquímicas de la mezcla natural de SLs y, por tanto, su eficiencia. Se utilizó melaza de azúcar como co-sustrato y espuma de poliuretano como soporte inerte. Se evaluó el efecto de la densidad de la espuma de poliuretano y la capacidad de retención hídrica y el proceso fue optimizado en base a la ratio de sustratos e inóculo obteniendo un rendimiento final de 0.211 g de SLs por g de sustrato. Los SLs producidos presentaron contenidos elevados de SLs diacetilados C18:0 acídico y lactónico. Se observaron correlaciones significativas entre el rendimiento de SLs y el oxígeno consumido (COA). Esto sugiere que el COA puede ser usado como medida indirecta de la producción de SLs para la monitorización on-line de procesos de FES. Esta tesis representa el comienzo de una nueva línea de investigación centrada en la producción de SLs por FES en el Grupo de Investigación en Compostaje (GICOM) del Departamento de Ingeniería Química, Biológica y Ambiental de la Universitat Autònoma de Barcelona.
This work proposes a potential alternative approach to produce sophorolipids (SLs), a type of biosurfactant, which are presented as an alternative to chemically-produced surfactants due to their higher efficiency and better environmental compatibility. Two strategies have been performed in this work to increase their cost-performance relative to petroleum based surfactants, which determines their commercial viability. Both are based in the production of SLs by the solid-state fermentation (SSF) of solid hydrophobic substrates by the yeast Starmerella bombicola. The first strategy was to use winterization oil cake (WOC), an oil cake that comes from the oil refining industry, to decrease the price of the substrates and, therefore, the final production costs of SLs. Sugar-beet molasses was used as co-substrate and wheat straw was chosen as inert support. The process was optimized in terms of substrates ratio, aeration rate and inoculum size at 0.5-L scale to obtain a yield of 0.261 g of SLs per g of substrate at day 10. The optimized process was successfully scale-up to a 40-L packed-bed bioreactor but problems associated with heat removal were found during the scale-up to a 100-L intermittently-mixed bioreactor. The chemical structure and interfacial properties of the SL natural mixture produced from the WOC were studied during a research stay at the Rensselaer Polytechnic Institute (RPI) in NY, USA. The second strategy consisted in the use of stearic acid (C18:0) to obtain SLs with a specific structure that improves the physicochemical properties of the SL natural mixture and, therefore, their performance. Sugar-beet molasses was used as co-substrate and polyurethane foam (PUF) functioned as inert support. The effect of PUF density and water holding capacity was assessed and the process was optimized in terms of substrate and inoculum ratio to obtain a final yield of 0.211 g of SLs per g of substrate. SLs produced herein had high contents of diacetylated acidic and lactonic C18:0 SLs. There were significant correlations between the SL yield and the oxygen consumed (COC). This suggests that the respiration parameter COC, can be used as an indirect measurement of the production of SLs for the on-line monitoring of SSF processes. This thesis represents the beginning of a new research line focused on the production of SLs by SSF in the Composting Research Group (GICOM) at the Department of Chemical, Biological and Environmental Engineering of the Universitat Autònoma de Barcelona.
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Weber, Andreas [Verfasser]. "Process Analysis of Biosurfactant Downstream Processing / Andreas Weber." München : Verlag Dr. Hut, 2014. http://d-nb.info/1063222281/34.
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Perfumo, Amedea. "Investigation of bacterial biosurfactant production for industrial use." Thesis, University of Ulster, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.554284.
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This study presents part of a multifaceted study of microbial biosurfactants and their industrial potential. The first step was the assembly of a microbial culture collection of a variety of biosurfactant-producing organisms with the choice of biosurfactant types informed by the industrial partner. The choice was based on two main criteria, the ease of product recovery and its final yield. Two groups of glycolipid biosurfactants were selected for further study: rhamnolipids produced by Pseudomonas aeruginosa and sophorolipids by Candida spp. Efforts to manipulate the biosurfactant chemical profiles by changing the cultivation media (carbon source in particular) and conditions in shake-flasks, demonstrated that there is only a limited possibility for changing the biosurfactant composition. This raised the question of the extent to which biosurfactant production is constrained by genetic determinants? To overcome the limitations of the flask-scale production, selected P. aeruginosa and Candida strains were used in bioreactors, and rhamnolipids and sophorolipids were synthesised in quantities sufficient for extraction and purification. The purified biosurfactants were used by the project partners for further characterisation and formulation in trial industrial products. Rhamnolipid yields were approximately 10 g/L whilst sophorolipid production exceeded 100 g/L. Detailed examination of the orcinol assay, which is widely used for the determination of rhamnolipid yields, showed that the method is flawed and provides an overestimate of yield when compared to quantification following extraction and purification. The culture approach had demonstrated the restricted possibilities for manipulating rhamnolipid production profile in P. aeruginosa and therefore a wider range of strains from different environmental niches were selected for genetic analysis. The aim of this part of the investigation was to establish the extent of natural gene variation which could be exploited for customised biosurfactant production. The comparative analysis of the rhl genes, coding the factors involved in rhamnolipid biosynthesis, was carried out on different P. aeruginosa strains isolated from water, soil and including pathogenic strains infecting cystic fibrosis patients. The extent of the gene sequence diversity resulted < 5%, which indicated that the rhl genes are conserved and are part of the core genome of P. aeruginosa. The single polymorphisms that occurred on the gene sequences, which gave rise to several variants, revealed no clear effect on the phenotype but appeared rather random. These variants showed also no specific correlation with different habitats. The analysis of the codon usage further supported the confidence in the highly conservative nature of the rhl genes. Most amino acids were encoded by highly preferred codons, some of which were selected upon the optimal translational efficiency, some others were instead determined by the high GC content of P. aeruginosa genome. On the whole, our data correlated well in showing that rhamnolipid biosurfactants produced by P. aeruginosa are conserved in their structure and profile as well in the genetic makeup. As involved in many important biological activities under a variety of environmental conditions, rhamnolipids developed as highly fit molecules. In the light of this, it appears that the likelihood that isolates of P. aeruginosa displaying unusual rhamnolipid profiles and features occurring naturally would be limited. Alternative routes for the development of industrially customised rhamnolipid biosurfactants are suggested as either the genetic manipulation of P. aeruginosa or the screening of rhamnolipid-producing organisms closely related.
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Urum, Kingsley. "Biosurfactant enhanced treatment of petroleum oil contaminated soils." Thesis, Heriot-Watt University, 2004. http://hdl.handle.net/10399/232.
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This thesis reports the experimental measurements on the ability of biological origin surfactants (i.e. biosurfactants - aescin, lecithin, rhamnolipid, saponin and tannin) on removing crude oil and a heavy fuel oil blend from various soils, through soil washing process. The greatest advantage of soil washing is that it is a physical means of separating oil from soil using water or surfactants without chemically modifying either the soil or the oil. The oil removal performance of the biosurfactants was evaluated against that of a well studied synthetic surfactant (sodium dodecyl sulphate, SDS) using water as a base case. For this purpose, different washing settings (i.e. test tubes, stirred flasks, packed column, and air bubble assisted stirred tank) were used to treat contaminated soils with high oil toxicity. The effects of operational parameters such as washing temperature (5 to 500C), washing time (1 to 20 minutes), concentration of surfactant solutions (0.004 to 0.5%-mass), volume of surfactant solution (5 to 20 cm3), flow rate (2 to 16 cm3/minutes), pore volume (10 to 70) and contamination history was investigated. The interaction of the surfactant solutions with the oil and soils was also investigated, which was used to explain the dominant mechanisms behind soil washing. The contaminated soils were prepared in the laboratory by mixing the oil and soils. Two different contamination cases were considered: weathered contamination in which freshly contaminated soils were subjected to heat treatment in a fan assisted oven (simulating weathering effect in the natural hot environments), and non-weathered contamination in which contaminated soils were not subjected to any heat treatment. The different washing techniques employed in this study yielded a novel and informative description on the selection of biosurfactants in the remediation of crude oil contaminated soils. This is believed to have major academic and industrial values for the treatment of (1) soil contaminated with oil, (2) sand produced with oil, (3) drill cuttings, (4) enhanced oil recovery, and (5) waste drilling mud and sludge from oil storage tank. In addition, the characterization of the biosurfactants in oil-water, soilwater and oil-soil systems give a general knowledge of their behaviour, which is important in the application for effective removal of oil from soil. Soil washing was found to have a considerable potential in removing oil from the different contaminated soils and results were comparable with those reported in literature. Oil removal by rhamnolipid was more effective than the other biosurfactants and water was effective at higher parameter levels. Further, biosurfactants can preferentially remove certain aromatic groups, which may be desirable for more rapid soil remediation. The rhamnolipid can be equally as efficient at removing oil from soil as SDS at a repeatability range of ± 6%. However, rhamnolipid have advantages over SDS beacuase the use of rhamnolipid will eliminate the need for removing surfactants from effluents as their release will not damage the environment due to their safe natures. Other surfactants (bio and/or synthetic) can be blended with rhamnolipid to achieve greater performance characteristics. In general, the stirred tank and air bubble assisted stirred tank reactors settings were more effective in removing oil from the weathered and the non-weathered contaminated soil samples. The most influential parameter on the oil removal was washing solution temperature with more than 80% of crude oil removal at 500C.
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Gidudu, Brian. "Biosurfactant Enhanced Bioelectrokinetic Remediation of Petrochemical Contaminated Soil." Diss., University of Pretoria, 2019. http://hdl.handle.net/2263/79238.
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Soil pollution in recent years has emerged as an issue of great environmental concern. Contamination of soil by improper disposal or spillage of petrochemicals and products containing petroleum hydrocarbons is one of such pollution cases highly reported. To remediate petroleum contaminated soil, A DC powered electrokinetic reactor was used with biosurfactants as an enhancement for the remediation process. To begin with, studies were made under voltage variations of 10 V and 30 V with an electrode spacing of 185 mm. Biosurfactant with its producing microbes and biosurfactant free cells were introduced in the soil chamber after which the reactor was left to run for 10 days under the electric field. The technology was able to achieve the highest oil recovery of 75.15 % from the soil in 96 hours at 30 V. With other factors remaining constant, the reactor was also operated under a constant voltage of 30 V with configurations of fixed electrodes spacings of 335 mm, 260 mm,185 mm and continuous approaching electrodes at 335 mm, 260 mm and 185 mm. The current in the electrolyte was highest with the least electrode distance of 185 mm. The increase in current led to a direct proportional increase in the electroosmotic flow towards the cathode leading to increased coalescence of the oil from the soil as compared to the other electrode distances. The analysis of the results showed reduction in the total carbon content in the soil with viable oil recovery rates for all the electrode distances with 185 mm being the most effective in both oil recovery and degradation. The reactor was further operated with amended biosurfactant concentrations of 28 g/L, 56 g/L and 84 g/L to enhance the recovery of oil from the soil and aid in biodegradation of the remaining oil by hydrocarbon degrading microbes. The highest oil recovery of 83.15 % was obtained with the biosurfactant concentration of 56 g/L showing that the hyper increase in concentration of the biosurfactants is not necessary to have an efficient process.
In all experiments the microorganisms were able to survive under the electro-halo-thermal environment in the reactor and degraded the remaining hydrocarbons to acceptable amounts in the environment. The bacteria were however affected by the constantly changing pH in all experiments. The presence of biosurfactants was so significant in aiding oil recovery and increasing bioavailability of hydrocarbons to the microbes. Production of biosurfactants in the reactor followed up by kinetic suggestions of the processes in the bioelectrokinetic reactor should be studied in future.Dissertation (MEng)--University of Pretoria, 2019.
Environmental Engineering
MEng
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Books on the topic "Biosurfactant"
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Kumar, Rajesh, and Amar Jyoti Das. Rhamnolipid Biosurfactant. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2.
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Soberón-Chávez, Gloria, ed. Biosurfactants. Berlin, Heidelberg: Springer Berlin Heidelberg, 2011. http://dx.doi.org/10.1007/978-3-642-14490-5.
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Sen, Ramkrishna, ed. Biosurfactants. New York, NY: Springer New York, 2010. http://dx.doi.org/10.1007/978-1-4419-5979-9.
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1966-, Sen Ramkrishna, ed. Biosurfactants. New York, N.Y: Springer Science+Business Media, 2010.
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Inamuddin, Mohd Imran Ahamed, and Ram Prasad, eds. Microbial Biosurfactants. Singapore: Springer Singapore, 2021. http://dx.doi.org/10.1007/978-981-15-6607-3.
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Sharma, Deepansh. Biosurfactants in Food. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-39415-2.
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1928-, Kosaric Naim, Cairns W. L. 1942-, and Gray, Neil C. C., 1954-, eds. Biosurfactants and biotechnology. New York: M. Dekker, 1987.
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Kumar, Pankaj, and Ramesh Chandra Dubey, eds. Multifunctional Microbial Biosurfactants. Cham: Springer Nature Switzerland, 2023. http://dx.doi.org/10.1007/978-3-031-31230-4.
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Aslam, Ruby, Mohammad Mobin, Jeenat Aslam, and Saman Zehra, eds. Advancements in Biosurfactants Research. Cham: Springer International Publishing, 2023. http://dx.doi.org/10.1007/978-3-031-21682-4.
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1928-, Kosaric Naim, ed. Biosurfactants: Production, properties, applications. New York: M. Dekker, 1993.
Find full textBook chapters on the topic "Biosurfactant"
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Kronemberger, Frederico de Araujo. "Biosurfactant." In Encyclopedia of Membranes, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-40872-4_61-1.
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Vadivel, Tamil Elakkiya, Krishnan Ravi Shankar, Tholan Gajendran, Theresa Veeranan, and Renganathan Sahadevan. "Biosurfactant." In Sustainable Bioprocessing for a Clean and Green Environment, 217–33. Boca Raton: CRC Press, 2021. http://dx.doi.org/10.1201/9781003035398-12.
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de Kronemberger, Frederico Araujo. "Biosurfactant Production." In Encyclopedia of Membranes, 1–2. Berlin, Heidelberg: Springer Berlin Heidelberg, 2014. http://dx.doi.org/10.1007/978-3-642-40872-4_62-1.
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Konwar, Bolin Kumar. "Biosurfactant Genetics." In Bacterial Biosurfactants, 49–70. Boca Raton: Apple Academic Press, 2022. http://dx.doi.org/10.1201/9781003188131-5.
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Kumar, Rajesh, and Amar Jyoti Das. "Rhamnolipid Biosurfactants and Their Properties." In Rhamnolipid Biosurfactant, 1–13. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2_1.
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Kumar, Rajesh, and Amar Jyoti Das. "Rhamnolipid-Assisted Synthesis of Stable Nanoparticles: A Green Approach." In Rhamnolipid Biosurfactant, 111–24. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2_10.
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Kumar, Rajesh, and Amar Jyoti Das. "Quorum Sensing: Its Role in Rhamnolipid Production." In Rhamnolipid Biosurfactant, 125–35. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2_11.
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Kumar, Rajesh, and Amar Jyoti Das. "Future Prospects and Scenario of Rhamnolipids." In Rhamnolipid Biosurfactant, 137–41. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2_12.
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Kumar, Rajesh, and Amar Jyoti Das. "Extraction, Detection, and Characterization of Rhamnolipid Biosurfactants from Microorganisms." In Rhamnolipid Biosurfactant, 15–28. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2_2.
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Kumar, Rajesh, and Amar Jyoti Das. "Production of Rhamnolipids." In Rhamnolipid Biosurfactant, 29–41. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-13-1289-2_3.
Full textConference papers on the topic "Biosurfactant"
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Baccile, Niki, Alexandre Poirier, and Chloe Seyrig. "Biosurfactants and biopolymers: Between interactions, orthogonality and mutual responsivity." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/taly8346.
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Polymer-surfactant systems have many applications in everyday life, but their petrochemical origin is not only controversial but soon submitted to regulatory restrictions. In a more environmentally friendly approach, but also to forecast regulations in both EU and USA, biopolymer-biosurfactant systems are an interesting alternative. There exists a large family of both biobased surfactants and polymers, which possess a huge potential, however currently limited, because both the solution behaviour of the former and their interactions are not established, yet. [1] This presentation shows the first set of work where biosurfactants (sophorolipids, glucolipids) are studied together with biopolymers (chitosan, alginate, gelatin, collagen, silk fibroin) (Figure 1). The results are astonishing, as they depend on the biosurfactant's self-assembled state, but also its charge and the charge of the biopolymer. Complex coacervates are often formed at basic pH when biosurfactants are within their micellar state and charges are opposite. [2] At acidic pH, orthogonality seems to prevail when the biosurfactant reorganizes into crystalline fibers, [3] but new phases, derived from mutual electrostatic interactions, can also appear, when the biosurfactants forms a vesicular phase. [4] These data are important to design biosurfactant-biopolymer-based nanocarriers or stimuli-responsive hydrogels. [1] Baccile N, Seyrig C, et al. Self-assembly, interfacial properties, interactions with macromolecules and molecular modelling and simulation of microbial bio-based amphiphiles (biosurfactants). A tutorial review. Green Chem. 2021, 3842[2] G. Ben Messaoud, Baccile N, et al. Complex coacervation of natural sophorolipid bolaamphiphile micelles with cationic polyelectrolytes. Green Chem. 2018, 3371[3] Seyrig C, Kignelman G, Thielemans W, Griel P Le, Cowieson N, Perez J, Baccile N et al. Stimuli-induced non-equilibrium phase transitions in polyelectrolyte-surfactant complex coacervates. Langmuir. 2020, 8839[4] Seyrig C, Baccile N et al. Synthesis of multilamellar walls vesicles polyelectrolyte-surfactant complexes from pH-stimulated phase transition using microbial biosurfactants. J Colloid Interface Sci. 2020, 493
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Bhattacharjee, Saurav, Borkha Mech, Naved Wasim Ahmed, Ankita Khataniar, and Aparoov Das. "Metagenomic Sequencing of Formation Water Sample of Upper Assam Oil Fields and Its Possible Applications in Microbial Enhanced Oil Recovery." In ADIPEC. SPE, 2023. http://dx.doi.org/10.2118/216577-ms.
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Abstract In this study, we describe a metagenomic approach which is a Next Generation Sequencing Technique (NGS) for the identification of biosurfactant-producing microbes present in the formation water sample in the oil fields of Upper Assam. This study also intends to investigate the possible use of the Bacillus subtilis OQ957160 strain (sb23) in the brown fields of Upper Assam for improving recovery of crude oil. Biosurfactant-producing strains like Bacillus Subtilis, Bacillus licheniformis, Pseudomonas Putida, and Pseudomonas aeruginosa were isolated using 16s rRNA gene sequencing and were described and examined based on their capacity to degrade crude oil to produce biosurfactants. Bacillus subtilis OQ957160 strain (sb23) was selected for further study based on its biosurfactant production capability and better interfacial tension reduction (IFT) and surface tension (ST) properties. Through changes to the growing environment, such as carbon source, temperature, pH, and salinity, the dynamics of growth research of the identified strain sb23 was done. Lipopeptide (Surfactin) was identified as the produced biosurfactant. After 70 hours of incubation under ideal conditions, the maximum biosurfactant production of 6000 mg/L and the minimum interfacial tension & surface tension of 0.98 and 23.8 mN/m were attained. At the value of 475 mg/L the biosurfactant solution exhibited critical micelle concentrations (CMC). Additionally, the biosurfactant exhibits outstanding surface activity throughout a wide temperature range of 35-95°C and at salinities ranging from 0.0-16.0% (w/v) and pH 2.0 -10.0. Under reservoir conditions, the produced biosurfactant from strain (sb23) is used in microbial flooding tests to recover an additional 7.85% of heavy crude oil. As a result, the isolated strain sb23 has the potential to significantly improve oil recovery from depleted oil fields of Upper Assam.
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Adeyemi, Gbadegesin Abiodun, Sven Egenhoff, Adesina Fadairo, kegang Ling, Olusegun Tomomewo, Adebowale Oladepo, Ayodeji Ayoola, and Jeffery Okonji. "Investigating Suitability of Microbial Derived Biosurfactant for Deliquefying Gas Well - An Experimental Approach." In SPE Nigeria Annual International Conference and Exhibition. SPE, 2023. http://dx.doi.org/10.2118/217253-ms.
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Abstract Liquid loading in the gas well is becoming more challenging as the gas field matured and may eventually kill the well if the liquids are not continuously removed from the well. The common method used in preventing liquid loading is by injecting surface-acting agents or solvents termed surfactants into the well to reduce the interfacial energy and weight of the water molecule in the gas stream Most surfactants used in the oil and gas industry today are synthetically manufactured which are toxic to life and environmentally incompatible. This paper presents a formulation of biosurfactant solution derived from Pseudomonas Aeruginosa and Escherichia Coli bacteria isolated from crude oil which is environmentally safe and evaluates the suitability fordeliquefying matured gas well. Generally, biosurfactants have the capacity to reduce the surface tension of the liquid by adsorbing at the liquid-gas interface and create significantly less mass than the liquid droplets which can then be easily extracted from the walls of the wellbore and assure flow in the gas system. In this study, the formulated biosurfactant was characterized for its physicochemical properties using scanning electron, microscopy (SEM), energy display spectroscopy (EDS), and Fourier transform infrared spectroscopy (FTIR). A specific experimental design was set up and used to evaluate the unloading efficiency of the formulated biosurfactant and then contrasted with Sodium Lauryl Sulphate, a widely used commercial surfactant (SLS). The bulk foam stability was tested, and the results obtained proved that biosurfactant from (Escherichia Coli) provided more stable foams (57.10%, 65.7%, 80.03%) as compared with commercial surfactant (SLS) (60.85%, 74.5%, 83.10%.) and biosurfactant from Pseudomonas Aeruginosa (12.85%, 8.57%, 4.28%) in the surfactant concentration of 30wt%, 40wt% and 50wt%. Also, the biosurfactant produced from Pseudomonas Aeruginosa and Escherichia Coli bacteria reduces the surface tension from which value of 65 mN/m to 48.4 mN/m and 21.9 mN/m respectively, compared to the commercial surfactant (SLS) value of 19.6 mN/m. This study has revealed that the two biosurfactants derived can create foam through which they decrease the density of the film at the wall, and alter the equilibrium between the gravitational force and the interfacial friction, hence making an easy transition between the churn flow and the annular flow to achieve at a lower flow rate. However, the biosurfactant produced from Escherichia Coli bacteria gave better surface tension results than biosurfactant from Pseudomonas aeruginosa and the results are closer to that of surfactant Sodium Lauryl Sulphate, a widely used commercial surfactant (SLS) used for validation.
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Alahmari, M. M., A. A. Humam, I. M. Zefzafy, C. Sanchez-Huerta, P. Y. Hong, and S. Zhang. "Hybrid Solution to Remediate Groundwater Contaminated by Petroleum-Hydrocarbons." In SPE Water Lifecycle Management Conference and Exhibition. SPE, 2024. http://dx.doi.org/10.2118/218976-ms.
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Abstract Groundwater contamination by petroleum-hydrocarbons is a serious environmental problem. Crude oil is a complex mixture of hydrocarbons with serious environmental and health risks. Thus, remediation of groundwater from petroleum hydrocarbon contamination is an emerging priority. Current approaches to remediate hydrocarbon contamination include physical and chemical methods. However, most of these approaches have a limited application for in-situ groundwater remediation. This study aims to develop a sustainable hybrid solution for efficient restoration of groundwater polluted by crude oil, providing a source of high-quality groundwater stream. Hybrid solution compromises in-situ addition of biosurfactant followed by a flow through electrochemical reactor installed in the groundwater well. The proposed hybrid solution comprises a two-stage process evaluated through lab-scale experiments treating crude oil that was mixed with synthetic water, mimicking groundwater contamination by petroleum-hydrocarbons. For biosurfactant optimization, glass flasks containing synthetic groundwater and crude oil were supplemented with biosurfactant BS, and C added at the three surfactants: oil (S:O) ratios 1:5, 1:10, and 1:50. Two temperature (25-35 °C) conditions were analyzed to simulate groundwater environment. The change in the crude oil layer thickness total petroleum hydrocarbon concentration (TPH) was continuously monitored for 60 days. Electron Oxidation was carried out where Boron-dopped diamond (BDD) anode and titanium cathode plates, were fitted into a 1 L reactor containing groundwater, crude oil and biosurfactant. Kinetic analysis at three constant currents (20, 30 and 40 mA/cm2) was performed. Samples were collected at regular intervals along 120 min to determine changes in TPH, COD and pH. The performance of biosurfactant BS and C in reducing the thickness of crude oil layer was influenced by different parameters including temperature, and S:O ratio. The increase in temperature further allowed higher effectiveness. For surfactant C, higher concentration of biosurfactant per unit of crude oil increased oil dispersion, the optimal S:O ratio of 1:5 allowed a maximal reduction of the crude oil layer of 27%. Biosurfactant BS, in contrast, presented optimal performance at ratio of 1:10 with a 30% reduction of the crude oil layer. Boron-doped diamond anode demonstrated high potential to oxidize TPH. The increased applied current from 20 to 40 mA enhanced the oxidation of COD and hydrocarbons (TPH ∼15-34%) along 120 min reaction. Addition of biosurfactant C resulted favorable COD and TPH oxidation. The proposed solution included adding biosurfactants followed by oxidation in a flow-through electrochemical reactor. Boron-doped diamond anode provided high electrochemical oxidation of COD and TPH, with an improved removal achieved when increasing the applied current from 20 to 40 mA and supplementing with biosurfactant C. The study provides a novel insight into enhanced bioremediation mechanism which is an integrated approach of EO and biosurfactant addition.
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Fitriyani, L. "Biosurfactant Addition into Solvent Extraction Process of Oily Contaminated Solid Waste." In Digital Technical Conference. Indonesian Petroleum Association, 2020. http://dx.doi.org/10.29118/ipa20-o-435.
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Solvent extraction has been used in industry or many purposes for years, including to recover oil at contaminated soil. Certain solvents and temperature ranges have been chosen to increase the oil recovery rate of extraction process. The Study observed the implementation of biosurfactant at the extraction process to perform reduction of total petroleum hydrocarbon (TPH) concentration of oily contaminated soil. In order to optimize TPH removal, extraction were conducted for multiple stages. Biosurfactant extraction result were also compared to solvent extraction process which acetone and toluene have been selected to extract oil content from contaminated soil by using solvent extraction process. The combination treatments with biosurfactant were also involving variety of centrifugation process with 1000 rpm (1570 g) operational speed. Duration of treatment process was 10 minutes with some variations of solid to solvent ratio. During the experiments comparison result between varies treatment process provides alternatives to treat oily contaminated soil by using extraction process. Compatibility among solvents, biosurfactants, types of oily contaminated solid waste were also observed to seek possibility on large scale of treatment process implementation both insitu at the contaminated site and exsitu at integrated waste treatment facility.
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Onaizi, Sagheer A. "Enzymatic Treatment of Phenolic Wastewater: Effects of Salinity and Biosurfactant Addition." In International Petroleum Technology Conference. IPTC, 2021. http://dx.doi.org/10.2523/iptc-21349-ms.
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Abstract Water contaminated with phenols is produced from several oil and gas related industries. Although there are a number of treatment methods, enzymatic wastewater treatment is more attractive due to its sustainability, environmental-friendliness, and mild nature. A key limitation of this process, however, is the enzymatic deactivation (whether complete or partial) during the treatment process. This limitation might be addressed to a certain extent through the addition of biosurfactants to the reaction medium. Thus, the key aim of this study is to utilize laccase (an oxidoreductase enzyme from Trametes versicolor) to remove bisphenol A (BPA) from wastewaters in the presence of rhamnolipid biosurfactant. Since most wastewaters contain inorganic salts, the efficacy of enzymatic treatment of high saline wastewaters has been evaluated. The beneficial effect of the biosurfactant addition during the enzymatic treatment of highly saline phenolic wastewater has been also assessed. Additionally, the effect of increasing the biocatalyst and the phenolic pollutant concentrations have been also probed. The results showed that the BPA degradation rate increases with increasing the enzyme concentration. The extent of BPA removal also increased with increasing the biocatalyst concentration, approaching almost a complete removal at an enzyme concentration of 400 ppm. The BPA degradation rate also increased almost linearly with increasing its initial concentration; however, its removal extent showed the opposite trend. The addition of as low as 1 ppm rhamnolipid biosurfactant to the reaction medium increased both the BPA degradation rate and the removal extent relative to the biosurfactant-free wastewater samples. The addition of the biosurfactant to the reaction medium boosted the BPA degradation rate and the removal extent by 1.1- to 1.23-fold. The highest BPA degradation rate and removal enhancement (about 23% higher than those in the absence of the biosurfactant) was obtained for BPA-rhamnolipid mass ratio of 50:1. The presence of salt severely reduced the BPA degradation rate and removal. The addition of 20 mM NaCl resulted in about 1.7-fold drop in the BPA degradation rate and removal. The drop in the BPA degradation rate and removal reached more than 3.6-fold at 500 mM NaCl. The addition of 1 ppm rhamnolipid partially compensated the negative effect of salinity, providing relatively higher BPA degradation rate and removal at all examined salinity levels. The findings reported herein reveal the positive effect of biosurfactant addition to the enzymatic reaction medium and the need for the salt removal prior to subjecting the saline wastewaters to enzymatic treatment.
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Melo, Palloma M. J. de, Nathália S. A. A. Marques, Adriana F. de Souza, Gabriela R. P. de Andrade, and Galba M. de Campos-Takaki. "Strategy for sustainable biosurfactant production by mucor circinelloides UCP0017." In III SEVEN INTERNATIONAL MULTIDISCIPLINARY CONGRESS. Seven Congress, 2023. http://dx.doi.org/10.56238/seveniiimulti2023-267.
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Surfactants are molecules with amphipathic structures, that is, one polar extreme (hydrophilic) and the other nonpolar (hydrophobic), possessing a high capacity to reduce surface tension, emulsification production and wetting (PELE et al., 2019; BIONE, 2019). Its origin can be chemical usually derived from petroleum, being compounds that easily harm the environment making it difficult to remove it (BIONE, 2019; GAYATHIRI et al., 2022). As they can also be natural, through microorganisms such as bacteria, filamentous fungi and yeasts or are biodegradable and of low toxicity, not harming the environment or human health. (RULLI et al., 2019; CÂNDIDO et al., 2022). Therefore, the present study aims to seek the optimization of the production of biosurfactant becoming a more advantageous alternative because they use renewable substrates in their composition, and because their "green" properties do not harm the environment, besides being biodegradable, thus improving their cost-benefit, aiming at new opportunities for applications in the food, agricultural, cosmetic and pharmaceutical industries (MARQUES et al., 2020; GAYATHIRI et al., 2022; MULLIGAN, 2023).The main responsible for the production of biosurfactants and bioemulsifiers are bacteria, followed by yeasts and finally filamentous fungi because they have a potential for the production of secondary metabolites and a high value of biomass, however, studies with filamentous fungi are little explored (SAŁEK & EUSTON, 2019; DERGUINE-MECHERI; KEBBOUCHE-GANA; DJENANE, 2021). According to Geethanjali et al., (2020) and Marques et al., (2020) the species Mucor circinelloides, of the phylum Mucoromycota, order Mucorales, presents a high biotechnological potential in the production of biomolecules of industrial interest, as well as biosurfactant. In this sense, the research proposed to study the performance of biosurfactant production through the filamentous fungus Mucor circinelloides UCP 0017 using alternative substrates (MARQUES et al., 2020; RADHA et al., 2020).
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Araújo, H. W. C., B. S. O. Ceballos, and G. M. Campos-Takaki. "Biosurfactant production by Chromobacterium prodigiosum." In Proceedings of the II International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2007). WORLD SCIENTIFIC, 2009. http://dx.doi.org/10.1142/9789812837554_0140.
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Coscia, Benjamin, Andrea Browning, Jeffrey Sanders, and Mat Halls. "Molecular simulation as a tool for the design of biosurfactant-based cosmetic formulations." In 2022 AOCS Annual Meeting & Expo. American Oil Chemists' Society (AOCS), 2022. http://dx.doi.org/10.21748/jdlz5827.
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There is growing consumer-driven demand in the cosmetic industry to replace petroleum-based surfactants with environmentally friendly alternatives. Biologically derived surfactants, or biosurfactants, are a promising class of molecules which may be suitable towards this end. However, separate from challenges in producing these materials which have been increasingly addressed throughout the last decade, formulations built around these new surfactants must perform competitively compared to existing formulations. There are a number of subclasses of biosurfactant molecules and modifications which can be made to those. The cosmetic science community is in need of lucid design principles for reformulating existing products using this set of ingredients. We believe that this presents an optimization challenge which molecular simulation is well-equipped to solve. In this work, we demonstrate how physics-based molecular simulation paired with chemistry-oriented informatics software can aid in the prospective design of new formulations. Simulation trivializes the enumeration of possible formulation compositions, allowing us to systematically study and gain a molecular-level understanding of surfactant aggregates at a variety of conditions. We test aqueous mixtures of four different types of biosurfactants at various concentrations and pH and present our analysis of aggregate size, shape and composition as well as solution properties such as viscosity and diffusivity.
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Au Yong, Hin Cheong, Kortney Tooker, Khanh Van Pham, Richard Arriaga, and Amir Mahmoudkhani. "Multifunctional Biosurfactants with Unusual pH Sensitive Interfacial Behavior for Remediation of Iron and Zinc Sulfide Formation Damage." In SPE International Conference on Oilfield Chemistry. SPE, 2023. http://dx.doi.org/10.2118/213799-ms.
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Abstract Metal sulfide scales are found in several fields in onshore and offshore oil and gas wells around the world. Although there has been some success in the development of sulfide scale inhibitors, significantly high concentration of inhibitor is often required specially to mitigate zinc sulfide. Microbial biosurfactants have an inherent affinity towards different mineral surfaces including sulfides. The unique surface and interfacial properties of these naturally derived products make them potential candidates for development of new products for metal sulfide scale management and control. In this work the properties of sophorolipids and rhamnolipids as dispersion and modification agents for iron and zinc sulfide precipitates were investigated. Surface and interfacial tension behaviors of microbial biosurfactants were measured using a drop shape tensiometer. Accelerated dispersion stability testing were used to determine the efficiency of biosurfactants for dispersing field collected and lab-made iron and zinc sulfides. Fourier transform – infrared (FTIR) and ultraviolet – visible (UV-vis) spectroscopy was used to determine the mode of interaction of the biosurfactant active sites with metal sulfide surfaces.
Reports on the topic "Biosurfactant"
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M.J. McInerney, S.K. Maudgalya, R. Knapp, and M. Folmsbee. DEVELOPMENT OF BIOSURFACTANT-MEDIATED OIL RECOVERY IN MODEL POROUS SYSTEMS AND COMPUTER SIMULATIONS OF BIOSURFACTANT-MEDIATED OIL RECOVERY. Office of Scientific and Technical Information (OSTI), May 2004. http://dx.doi.org/10.2172/834170.
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M.J. McInerney, R.M. Knapp, Kathleen Duncan, D.R. Simpson, N. Youssef, N. Ravi, M.J. Folmsbee, et al. Development of an In Situ Biosurfactant Production Technology for Enhanced Oil Recovery. Office of Scientific and Technical Information (OSTI), September 2007. http://dx.doi.org/10.2172/943328.
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M.J. McInerney, N. Youssef, T. Fincher, S.K. Maudgalya, M.J. Folmsbee, R. Knapp, and D. Nagle. DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY. Office of Scientific and Technical Information (OSTI), May 2004. http://dx.doi.org/10.2172/834168.
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M.J. McInerney, K.E. Duncan, N. Youssef, T. Fincher, S.K. Maudgalya, M.J. Folmsbee, R. Knapp, Randy R. Simpson, N.Ravi, and D. Nagle. Development of Microorganisms with Improved Transport and Biosurfactant Activity for Enhanced Oil Recovery. Office of Scientific and Technical Information (OSTI), August 2005. http://dx.doi.org/10.2172/860919.
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M.J. McInerney, R.M. Knapp, Jr D.P. Nagle, Kathleen Duncan, N. Youssef, M.J. Folmsbee, and S. Maudgakya. DEVELOPMENT OF MICROORGANISMS WITH IMPROVED TRANSPORT AND BIOSURFACTANT ACTIVITY FOR ENHANCED OIL RECOVERY. Office of Scientific and Technical Information (OSTI), June 2003. http://dx.doi.org/10.2172/822122.
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Katan, Jaacov, and Michael E. Stanghellini. Clinical (Major) and Subclinical (Minor) Root-Infecting Pathogens in Plant Growth Substrates, and Integrated Strategies for their Control. United States Department of Agriculture, October 1993. http://dx.doi.org/10.32747/1993.7568089.bard.
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In intensive agriculture, harmful soilborne biotic agents, cause severe damage. These include both typical soilborne (clinical) major pathogens which destroy plants (e.g. Fusarium and Phytophthora pathogens), and subclinical ("minor") pathogens (e.g. Olpidium and Pythium). The latter cause growth retardation and yield decline. The objectives of this study were: (1) To study the behavior of clinical (major) and subclinical (minor) pathogens in plant growth substrate, with emphasis on zoosporic fungi, such as Pythium, Olipidium and Polymyxa. (2) To study the interaction between subclinical pathogens and plants, and those aspects of Pythium biology which are relevant to these systems. (3) To adopt a holistic-integrated approach for control that includes both eradicative and protective measures, based on a knowledge of the pathogens' biology. Zoospores were demonstrated as the primary, if not the sole propagule, responsible for pathogen spread in a recirculating hydroponic cultural system, as verified with P. aphanidermatum and Phytophthora capsici. P. aphanidermatum, in contrast to Phytophthora capsici, can also spread by hyphae from plant-to-plant. Synthetic surfactants, when added to the recirculating nutrient solutions provided 100% control of root rot of peppers by these fungi without any detrimental effects on plant growth or yield. A bacterium which produced a biosurfactant was proved as efficacious as synthetic surfactants in the control of zoosporic plant pathogens in the recirculating hydroponic cultural system. The biosurfactant was identified as a rhamnolipid. Olpidium and Polymyxa are widespread and were determined as subclinical pathogens since they cause growth retardation but no plant mortality. Pythium can induce both phenomena and is an occasional subclinical pathogen. Physiological and ultrastructural studies of the interaction between Olpidium and melon plants showed that this pathogen is not destructive but affects root hairs, respiration and plant nutrition. The infected roots constitute an amplified sink competing with the shoots and eventually leading to growth retardation. Space solarization, by solar heating of the greenhouse, is effective in the sanitation of the greenhouse from residual inoculum and should be used as a component in disease management, along with other strategies.
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M.J. McInerney, M. Folmsbee, and D. Nagle. DEVELOPMENT OF IMPROVED ANAEROBIC GROWTH OF BACILLUS MOJAVENSIS STRAIN JF-2 FOR THE PURPOSE OF IMPROVED ANAEROBIC BIOSURFACTANT PRODUCTION FOR ENHANCED OIL RECOVERY. Office of Scientific and Technical Information (OSTI), May 2004. http://dx.doi.org/10.2172/834171.
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Spetzler, Hartmut. Seismic Absorption and Modulus Measurements in Porous Rocks in Lab and Field: Physical, Chemical, and Biological Effects of Fluids (Detecting a Biosurfactant Additive in a Field Irrigation Experiment). Office of Scientific and Technical Information (OSTI), May 2006. http://dx.doi.org/10.2172/1010627.
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McInerney, J. J., S. O. Han, S. Maudgalya, H. Mouttaki, M. Folmsbee, R. Knapp, D. Nagle, B. E. Jackson, M. Stuadt, and W. Frey. Development of More Effective Biosurfactants for Enhanced Oil Recovery. Office of Scientific and Technical Information (OSTI), January 2003. http://dx.doi.org/10.2172/806980.
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McInerney, M. J., H. Mouttaki, M. Folmsbee, R. Knapp, and D. Nagle. Development of More Effective Biosurfactants for Enhanced Oil Recovery. Office of Scientific and Technical Information (OSTI), January 2003. http://dx.doi.org/10.2172/807189.
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