To see the other types of publications on this topic, follow the link: Biosurfactant.
Journal articles on the topic 'Biosurfactant'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Biosurfactant.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Brinda, C. M., R. Ragunathan R. Ragunathan, and Jesteena Johney. "Diversity and Distribution of Potential Biosurfactant Producing Bacillus Sp MN 243657, GC-MS Analysis and its Antimicrobial Study." Biosciences Biotechnology Research Asia 20, no. 1 (March 30, 2023): 271–91. http://dx.doi.org/10.13005/bbra/3088.
Full textAbstract:
Biosurfactants are microbially produced surface-active compounds. They are amphiphilic molecules with hydrophilic and hydrophobic regions. The demand for biosurfactants has been exponentially growing as they are nontoxic and biodegradable. They have different applications in several industrial sectors. The objective of this study was to isolate and characterize the native bacteria which produce biosurfactants from oil contaminated soil of different places in Kerala and Tamil Nadu, India. The soil samples were collected from petrol pumps and workshops where the soil is contaminated with petrol, diesel and oil. The bacteria were isolated from contaminated soil samples and confirmed as Bacillus sp.The cultures were screened for biosurfactant production by different screening techniques such as blood hemolysis, oil spreading assay, emulsification ability assay, bacterial adherence to hydrocarbons activity. The potential biosurfactant producing culture was selected and identified using molecular techniques and submitted to NCBI Gene Bank (MN 243657 – Bacillus sp). The selected bacterial culture was used for biosurfactant production and these were characterized by UV, TLC, FTIR and GC -MS analysis. The derived biosurfactant's Rf value was 0.68 as determined by a TLC chromatogram. In a UV-visible spectroscopy study, the isolated biosurfactant displayed a highest peak at 415 nm. According to FTIR analysis, the isolated biosurfactant displayed an intense peak at 3340 cm -1. The large peaks of the biosurfactant were observed at various retention times of 12.75, 10.22, 4.98, and 3.87, respectively, after GC-MS analysis. Antibacterial and antifungal activity of the biosurfactant was identified against pathogenic bacteria such as P.aeruginosa, E. coli, K. pneumoniae, S. aureus and fungi Aspergillus niger, Aspergillus terreus, Aspergillus flavus.
2
Liepins, Janis, Karina Balina, Raimonda Soloha, Ieva Berzina, Liva Kristiana Lukasa, and Elina Dace. "Glycolipid Biosurfactant Production from Waste Cooking Oils by Yeast: Review of Substrates, Producers and Products." Fermentation 7, no. 3 (July 29, 2021): 136. http://dx.doi.org/10.3390/fermentation7030136.
Full textAbstract:
Biosurfactants are a microbially synthesized alternative to synthetic surfactants, one of the most important bulk chemicals. Some yeast species are proven to be exceptional biosurfactant producers, while others are emerging producers. A set of factors affects the type, amount, and properties of the biosurfactant produced, as well as the environmental impact and costs of biosurfactant’s production. Exploring waste cooking oil as a substrate for biosurfactants’ production serves as an effective cost-cutting strategy, yet it has some limitations. This review explores the existing knowledge on utilizing waste cooking oil as a feedstock to produce glycolipid biosurfactants by yeast. The review focuses specifically on the differences created by using raw cooking oil or waste cooking oil as the substrate on the ability of various yeast species to synthesize sophorolipids, rhamnolipids, mannosylerythritol lipids, and other glycolipids and the substrate’s impact on the composition, properties, and limitations in the application of biosurfactants.
3
Oyedeji, Olaoluwa, Deborah Ifeoluwa Onifade, and Anthony Abiodun Onilude. "Production, Characterization, and Application of Biosurfactant From Lactobacillus plantarum OG8 Isolated From Fermenting Maize (Zea Mays) Slurry." Acta Universitatis Cibiniensis. Series E: Food Technology 26, no. 2 (December 1, 2022): 271–86. http://dx.doi.org/10.2478/aucft-2022-0022.
Full textAbstract:
Abstract Biosurfactants have wide applications in several industries. However, high production costs and safety concerns have limited their comprehensive use. Twenty-five strains of lactic acid bacteria, isolated from fermenting maize slurry, were screened for biosurfactant production using the emulsification activity (E24) assay. The selected bacterium was identified molecularly using the 16S rRNA gene sequencing as Lactobacillus plantarum OG8. The effect of some cultural factors on biosurfactant production from the bacterium, using pineapple peel as a low-cost substrate, was investigated. The optimum yield of biosurfactant occurred at a 48 h incubation period, using glucose and peptone as carbon and nitrogen sources, respectively. The biosurfactant was characterized to possess mostly carbohydrates, followed by protein and lipid contents. Optima pH 10.0 and temperature 60 °C were the best for the biosurfactant activity. The biosurfactant exhibited antimicrobial activity against bacterial pathogens Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae, at a concentration of 5.0 mg/mL. The use of pineapple peel as a low-cost substrate for biosurfactant production from Lactobacillus plantarum OG8 will serve for cost-effective production. The biosurfactantt produced exhibited promising properties such as thermostability and antimicrobial activity against food spoilage and pathogenes that could make it suitable for food processing and preservation.
4
Amraini, Said Zul, Sri Rezeki Muria, Bahruddin Bahruddin, Irdoni HS, Ulfa Dwi Artha, and Reno Susanto. "Biosurfactant Production from Pseudomonas aeruginosa ATCC27853 with Carbon Source from Crude Palm Oil for Oil Recovery." Indo. J. Chem. Res. 10, no. 1 (May 31, 2022): 47–52. http://dx.doi.org/10.30598/ijcr.2022.10-sai.
Full textAbstract:
Biosurfactants are surfactants that are synthesized by microorganisms using organic materials and have biodegradable properties, making them environmentally friendly. One of the applications of biosurfactants in the recovery of petroleum. This study aims to determine the type of biosurfactant produced by Pseudomonas aeruginosa bacteria using crude palm oil as the main carbon source, to determine the effect of variations in pH and CPO concentration on surface tension reduction and emulsification, and compare the best biosurfactant with surfactant synthesis. The production of biosurfactants has 3 stages, namely the bacterial preparation, the biosurfactant production, and the analysis in the form of surface tension, emulsification, crude oil removal, and FTIR. The best biosurfactant was obtained at pH 7 and a carbon source concentration of 3% v/v with surface tension and emulsification values of 42.49 mN/m and 58%, respectively. The pH value and CPO concentration can affect the growth in the biosurfactant production process, thus also affecting the surface tension and emulsification values. The biosurfactants obtained were rhamnolipid biosurfactants. The biosurfactants produced in this study have lower crude oil recovery capabilities than synthetic surfactants with crude oil removal values of 57.78% and 79.34%, respectively.
5
Gunjal, Aparna. "Biosurfactants from renewable sources - A review." Nepal Journal of Environmental Science 10, no. 2 (December 31, 2022): 15–23. http://dx.doi.org/10.3126/njes.v10i2.48538.
Full textAbstract:
Biosurfactants have wide applications in pharmaceutical, agriculture and food industries. The research area of biosurfactants is gaining immense attention. The review mentions here the advantages and various substrates used for biosurfactants production. The pre-treatment of substrates for biosurfactants production is also focused. The production of biosurfactants by solid state fermentation is also described. The renewable substrates, yield and microorganisms used for biosurfactant production are also taken into consideration. The screening methods for biosurfactant are also described. The use of renewable sources for biosurfactant production is specially focused in the review. This will be very eco-friendly, easy and economical. More studies need to BE done on large-scale production of biosurfactants using genetically engineered microorganisms.
6
Youssef, Noha H., Kathleen E. Duncan, and Michael J. McInerney. "Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity." Applied and Environmental Microbiology 71, no. 12 (December 2005): 7690–95. http://dx.doi.org/10.1128/aem.71.12.7690-7695.2005.
Full textAbstract:
ABSTRACT Biosurfactant production may be an economic approach to improving oil recovery. To obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus Bacillus, were tested for growth and biosurfactant production in medium with 5% NaCl under aerobic and anaerobic conditions. All strains grew aerobically with 5% NaCl, and 147 strains produced a biosurfactant. Thirty-five strains grew anaerobically with 5% NaCl, and two produced a biosurfactant. In order to relate structural differences to activity, eight lipopeptide biosurfactants with different specific activities produced by various Bacillus species were purified by a new protocol. The amino acid compositions of the eight lipopeptides were the same (Glu/Gln:Asp/Asn:Val:Leu, 1:1:1:4), but the fatty acid compositions differed. Multiple regression analysis showed that the specific biosurfactant activity depended on the ratios of both iso to normal even-numbered fatty acids and anteiso to iso odd-numbered fatty acids. A multiple regression model accurately predicted the specific biosurfactant activities of four newly purified biosurfactants (r 2 = 0.91). The fatty acid composition of the biosurfactant produced by Bacillus subtilis subsp. subtilis strain T89-42 was altered by the addition of branched-chain amino acids to the growth medium. The specific activities of biosurfactants produced in cultures with different amino acid additions were accurately predicted by the multiple regression model derived from the fatty acid compositions (r 2 = 0.95). Our work shows that many strains of Bacillus mojavensis and Bacillus subtilis produce biosurfactants and that the fatty acid composition is important for biosurfactant activity.
7
Javadi, Ali, Mohamad Reza Pourmand, Javad Hamedi, Fatemeh Gharebaghi, Zohre Baseri, Razieh Mohammadzadeh, and Seyyed Saeed Eshraghi. "Evaluation of anti-biofilm potential of biosurfactant extracted from Nocardia species." Folia Medica 63, no. 3 (June 30, 2021): 392–99. http://dx.doi.org/10.3897/folmed.63.e54386.
Full textAbstract:
Introduction: Bacterial natural products such as biosurfactants and surface-active agents are important compounds which exhibit many applications in the fields of medicine. Aim: The aim of the present study was to isolate and identify Nocardia strains with high biosurfactant production and antibiofilm ability. Materials and methods: In the present study, a biosurfactant producing Nocardia species was isolated and identified by a laboratory method. Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The oil spreading test and the surface tension measurements showed that one strain was a biosurfactant producer. The strain with the best surface activity results was selected for further studies and identified by 16S rRNA gene sequencing method. Fourier transform infrared spectroscopy (FTIR) and compositional analysis proved a biosurfactant structure. Results: Oil spreading test and blue agar plate test confirmed biosurfactants and extracellular anionic glycolipids. E24% assay using olive oil revealed strong emulsifying characteristic of the extracted biosurfactant with 100% emulsifying strength. FTIR spectrum indicated the presence of aliphatic hydrocarbon chain (lipid) along with the polysaccharide portion, confirming the glycolipid nature of the biosurfactant. The stability of the biosurfactant produced in different conditions was significant. Increasing concentration of BS significantly inhibited Pseudomonas aeruginosa biofilm. Conclusions: N. coubleae can be a representative of the genus Nocardia for the production of biosurfactants with beneficial physicochemical properties.
8
Fachria, Rizqy. "APLIKASI BIOSURFAKTAN Bacillus subtilis ATCC 19659 DENGAN MEDIA PRODUKSI LIMBAH TAHU UNTUK ENHACED OIL RECOVERY." Jurnal Teknologi Lingkungan Lahan Basah 9, no. 2 (August 29, 2021): 101. http://dx.doi.org/10.26418/jtllb.v9i2.48221.
Full textAbstract:
Biosurfactant as secondary metabolit produced by Bacillus subtilis. It has the ability to emulsify and reduce the surface tension. Biosurfactants produced by B. subtilis is a lipopeptide. Furthermore, biosurfactant can be utilized in microbial enhanced oil recovery (MEOR). In this research, biosurfactant of B. subtilis ATCC 19 659 were evaluated. The production use Nutrient Broth (NB) and soybean liquid waste. Application of biosurfactant in oil recovery showed that biosurfactant of NB recover 2 mL crude oil and biosurfactant of soybean liquid waste medium recover 3.67 mL.
9
Sena, Hellen Holanda, Michele Alves Sanches, Diego Fernando Silva Rocha, Walter Oliva Pinto Filho Segundo, Érica Simplício de Souza, and João Vicente Braga de Souza. "Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest." International Journal of Microbiology 2018 (2018): 1–8. http://dx.doi.org/10.1155/2018/5684261.
Full textAbstract:
Biosurfactants are surface-active compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the Amazon forest. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. In addition, the influences of bioprocess factors (carbon source, nitrogen source, pH, and fermentation time) were investigated. Finally, the biosurfactant produced was semipurified and submitted to stability tests. One hundred fungal cultures were obtained from the soil samples, identified by micromorphology, and submitted to screening for biosurfactant production. Sixty-one strains produced biosurfactants. The strainPenicillium8CC2 showed the highest emulsification index (54.2%). The optimized bioprocess conditions for biosurfactant production byPenicillium8CC2 were as follows: soybean oil, 20 g/L; yeast extract, 30 g/L; pH 9; duration of 9 days. The semipurified biosurfactant showed stability after heating at 100°C for 60 min and after the addition of 30% NaCl (w/v). Tween 80 (0.2% w/v), a conventional surfactant, was used as the control.
10
Vaijayanti, Mahulkar Ankita Vidyadhar. "Comparative study of antimicrobial efficiency of biosurfactant producing Pseudomonas spp. from different soil samples." Journal of Applied and Advanced Research 5 (September 6, 2020): 1. http://dx.doi.org/10.21839/jaar.2020.v5.318.
Full textAbstract:
Amphiphilic biosurfactants are surface-active biological molecules secreted by hydrocarbanoclastic microorganisms. Biosurfactants are eco-friendly, less toxic, biodegradable, and low-cost material, so it has more advantages over chemical surfactants. In this research, Pseudomonas spp., biosurfactant producing microorganisms isolated from different sources of soil samples. IS1, IS2, IS3, IS4 isolates obtained from Garden soil sample; Metal contaminated soil sample; Petroleum contaminated soil sample; Oil contaminated soil sample; respectively. Each isolates identified as Pseudomonas spp. Furthermore, screened for biosurfactant producers. Each isolate showed positive results for the hemolysis test, drop collapse test, oil displacement test, and emulsification test. All isolate incubated in mineral salt medium for biosurfactant production. Biosurfactant extracted from IS1, IS2, IS3, IS4 showed 35%, 65%, 20%, 52% emulsification index respectively. Antimicrobial activity of extracted biosurfactants against pathogenic microorganisms checked by agar cup method. IS2 isolate shows the highest antimicrobial activity among all. All isolate showed a higher zone of inhibition against gram-positive microorganisms than gram-negative microbes. The purpose of this study involves the assessment of the antimicrobial activity of biosurfactant producers from the soil environment.
11
ADEBAJO, S. O., A. K. AKINTOKUN, A. E. OJO, and C. A. AKINREMI. "STABILITY OF BIOSURFACTANT PRODUCED BY PSEUDOMONAS TAENENSIS." Journal of Natural Sciences Engineering and Technology 18, no. 1 (October 6, 2020): 155–65. http://dx.doi.org/10.51406/jnset.v18i1.2039.
Full textAbstract:
Biosurfactants are one of the microbial bioproducts that are naturally synthesized and are applicable for many industrial purposes. In this study, antibacterial, stability and antibiotic susceptibility of biosurfactant was evaluated. Biosurfactants produced from different substrates (groundnut cake, cassava flour waste, pome, cooking oil, engine oil, cassava waste water, molasses, cassava peel, potato) by Pseudomonas taenensis were evaluated for antibacterial activity using agar well diffusion method. Antibiotics susceptibility of Pseudomonas taenensis was carried out using different antibiotics (augmentin, ofloxacin, tetracyclin and ciprofloxacin, cotrimoxazole, pefloxacin, amoxylin, ceftriazone, nitrofuranton and gentamycin). The stability of the biosurfactant was evaluated by adjusting the biosurfactant to: pH (2, 4, 6, 8, 10 and 12) using 1M NaOH and 1M HCl, temperature (4, 30, 37, 55, 75 and 100 °C) and NaCl (0, 5, 10, 15, 20 and 25 %). Results showed that only biosurfactant produced using cassava waste water as substrate was sensitive to Escherichia coli while biosurfactant produced using cassava flour waste, pome and molasses were sensitive to Staphylococcus aureus. Biosurfactant-producing isolate (Pseudomonas taenensis) was sensitive to four antibiotics (augmentin, ofloxacin, tetracyclin and ciprofloxacin) and resistant to six antibiotics (cotrimoxazole, pefloxacin, amoxylin, ceftriazone, nitrofuranton and gentamycin). Biosurfactant was stable over all the wide ranges of pH, temperature and sodium chloride concentrations investigated. This study therefore revealed that biosurfactant have good stability, thus, could survive environmental stress; Not all biosurfactant and biosurfactant producers have antimicrobial and antibiotic property.
12
Tadayon Tajabadi, Mohammadhassan, Asyeih Sabernejad, and Mohsen Khalili Najafabadi. "Biosurfactant-producing Microorganisms: Potential for Bioremediation of Organic and Inorganic Pollutants." Research in Biotechnology and Environmental Science 2, no. 2 (June 16, 2023): 18–23. http://dx.doi.org/10.58803/rbes.v2i2.13.
Full textAbstract:
The contamination of soil and water by heavy metals and hydrophobic organic compounds poses a significant threat to the environment. Traditional physicochemical methods for remediation are often expensive and environmentally unfriendly, while bioremediation offers a more eco-compatible and economically feasible alternative. Bioremediation utilizes microorganisms, plants, or microbial/plant enzymes to detoxify contaminants in various environments. Biosurfactants, amphiphilic compounds produced by microorganisms, play a crucial role in enhancing bioremediation effectiveness. They increase substrate surface area, create microenvironments, and promote emulsification, thereby facilitating the removal of pollutants. This article provided a comprehensive overview of biosurfactant-producing microorganisms and their potential in the bioremediation of organic and inorganic pollutants. The types and classifications of biosurfactants as well as the factors influencing their production were discussed. Various microorganisms, including bacteria, fungi, and yeasts, were identified as biosurfactant producers. This study outlined the production process and highlighted the importance of optimizing growth conditions for high-quality biosurfactant production. The applications of biosurfactants in remediation were explored by emphasizing their ability to enhance biodegradation, remove heavy metals, and increase hydrocarbon bioavailability. Several studies demonstrating the efficacy of biosurfactant-producing microorganisms in bioremediation were presented. The potential limitations and challenges associated with biosurfactant application in situ were also discussed. In conclusion, the controlled use of biosurfactants could offer promising prospects for the efficient and sustainable cleanup of contaminated sites, contributing to environmental remediation efforts.
13
Onlamool, Theerawat, Atipan Saimmai, and Suppasil Maneerat. "Antifungal Activity of Rhamnolipid Biosurfactant Produced by Pseudomonas aeruginosa A4 against Plant Pathogenic Fungi." Trends in Sciences 20, no. 3 (December 28, 2022): 6524. http://dx.doi.org/10.48048/tis.2023.6524.
Full textAbstract:
Biosurfactants have been shown to have a variety of other agricultural applications. Antimicrobial activity is a desirable property in a variety of biosurfactants. Several biosurfactants produced from bacteria have antibacterial activity against plant diseases, making them a promising biocontrol molecule for ensuring agricultural sustainability in the long run. The purpose of this research was to characterize biosurfactant produced at different carbon source by biosurfactant-producing bacterial strains isolated from contaminated soapstock sediment, as well as its antifungal properties. Biosurfactant-producing bacteria were isolated from soapstock-contaminated soil which bacteria were selected by using drop collapse and oil displacement tests. Twelve isolates reduced surface tension of culture broth from 50 to 31 - 43 mN/m. According to 16S rRNA sequence analysis, these isolates belong to 8 different genera (Acinetobacter, Citrobacter, Enterobacter, Klebsiella, Pantoea, Pseudomonas, Stenotrophomonas and Xanthomonas). The supernatant of Pseudomonas aeruginosa A4 grown in MSM supplemented with soapstock was the most effective biosurfactant against Aspergillus flavus F2, Aspergillus niger F14, Cunninghamella bertholletiae F1 and Rhizopus oryzae F5, inhibiting mycelium growth by 54, 61 59 and 50 %, respectively. The extract substance inhibited spore germination against A. flavus F2 and R. oryzae F5 with a minimum inhibitory concentration of 2.75 mg/mL. TLC, FT-IR, ESI-MS and GC-MS analysis demonstrated that the mono-rhamnolipids and di-rhamnolipids had the same 3-hydroxy fatty acid composition of C8, C10 and C12. Overall, the biosurfactant-producing strains identified in this investigation show promise for future development and application in cost-effective industrial-scale biotechnological processes.
HIGHLIGHTS
Biosurfactant producing bacteria were isolated from soapstock contaminated soil
The supernatant of Pseudomonas aeruginosa A4 grown in MSM supplemented with soapstock was the most effective biosurfactant against plant pathogenic fungi
Biosurfactant from Pseudomonas aeruginosa A4 was identified as rhamnolipid comprising of both mono and di-rhamnolipid congeners
GRAPHICAL ABSTRACT
14
Shaileshbhai, Mr Gopani Juvin, and Ms Trupti Pandya. "Microbial Production Bioemulsifier and its Applications." International Journal for Research in Applied Science and Engineering Technology 11, no. 7 (July 31, 2023): 637–47. http://dx.doi.org/10.22214/ijraset.2023.54727.
Full textAbstract:
Abstract: According to various research, Biosurfactants are generally composed of amphipathic motes that have both hydrophilic and hydrophobic ingredients. The hydrophilic composites generally correspond to positive, negative, or amphoteric charged ions, whereas the hydrophobic composites are made up of a long chain of fatty acids. It is getting important in biotechnology products for numerous industrial applications including in food, cosmetics and cleaning products, medicine, drug, and oil and gas. Bacterial cells produce an admixture of biosurfactant( BS) lipids with the help of which oil is dispersed into veritably fine droplets and therefore the bioavailability of CO is increased. Biosurfactants are surface-active site compounds produced by microorganisms. Biosurfactant generally refers to surfactants of microbial origin. Most of the biosurfactants produced by microbes are synthesized extracellularly and numerous microbes are known to produce biosurfactants in large relative amounts. This study concentrates on the insulation of biosurfactant-producing microorganisms from soil samples.
15
Tabrizi Rayeni, Laya, and Shahla Soltani Nezhad. "Characterization of Biosurfactant Produced by Probiotic Bacteria Isolated from Human Breast Milk." International Journal of Basic Science in Medicine 3, no. 1 (February 14, 2018): 18–24. http://dx.doi.org/10.15171/ijbsm.2018.04.
Full textAbstract:
Background: Breast milk is an important nutrient source for rapidly growing neonates since breastfeeding protects the newborn against some disease. This effect may be due to the useful and natural microflora of breast milk. Biosurfactants are unique amphipathic compounds produced by some microorganisms. The present study demonstrates the isolation and characterization of biosurfactant producing bacteria from human breast milk samples. Methods: The human breast milk samples were collected aseptically and then cultured in MRS agar media. The biosurfactant producing ability of the isolated strains was investigated by hemolytic assay, oil spreading method, drop collapse test and emulsification index assay. The screened isolates were identified by 16S rDNA gene sequencing analysis. In vitro antibacterial activities of biosurfactants against some Gram-positive and Gram negative bacteria were investigated by the agar disc diffusion method. This biosurfactant was characterized with Fourier transform infrared spectroscopy (FTIR). Results: In this study, 337 different colonies were isolated from 42 breast milk samples. The best isolates were identified as Pediococcus pentosaceus HM-1, Pediococcus pentosaceus HM-2 and Pediococcus pentosaceus HM-3 based on microscopic and 16S rDNA gene sequencing analysis. The biosurfactant extracted from screened strains exhibited a broad spectrum of antagonistic activity against some pathogenic bacteria. The results showed similarity to lipopeptide biosurfactants like surfactin. Conclusion: Bacterial strains isolated in this study could be valuable sources for novel biosurfactants. The Human breast milk could be a safe source for isolation of biosurfactant producing probiotic bacteria and for improve intestinal microflora of infants.
16
Ortansa, Csutak, Sarbu Ionela, Rusu Elena, Tatiana Vassu, and Viorica Corbu. "Antimicrobial and Antiadhesion Activity of Biosurfactants from Rhodotorula glutinis Grown on n-dodecane." Revista de Chimie 71, no. 5 (May 29, 2020): 99–105. http://dx.doi.org/10.37358/rc.20.5.8117.
Full textAbstract:
The biosurfactants represent a class of biocompounds of growing interest for biomedical applications. The yeast strain Rhodotorula glutinis CMGB-RG1 assimilated normal dodecane (C12H26) with high rates, producing biosurfactants with constant emulsification index (29%) after two, respectively, three days. Best antimicrobial activity was recorded for the [50X] concentrated biosurfactant against Candida krusei CMGB-Y8 from vaginal infections. The crude biosurfactant showed high microbial inhibition of biofilms formed by Candida krusei CMGB 94 (35%), while for C. krusei CMGB-Y8 best results were obtained with [50X] concentrated biosurfactant (47%) due to the presence of more effective mechanism for biofilm formation and adhesion. The results represent a basis for further studies regarding the characterization of antimicrobial mechanism of action of R. glutinis biosurfactants against a large range of human pathogenic microorganisms.
17
Osmorskaya, Zlata, Nikita Ezhkin, Alexander Gordeev, Liliya Biktasheva, and Svetlana Selivanovskaya. "Characterization of biosurfactants produced by the Bacillus amyloliquefaciens ВКМА В-12464." E3S Web of Conferences 392 (2023): 01013. http://dx.doi.org/10.1051/e3sconf/202339201013.
Full textAbstract:
Biosurfactants are amphiphilic substances with a wide range of uses. Biosurfactants are being researched in various areas of biotechnology. They are especially widely used in the development of new environmentally friendly methods for increasing oil production, as well as in agriculture as an effective biocontrol agent. However, the possibility of finding strains producing biosurfactants and the efficiency of their production is a separate task for research. The aim of this work was to evaluate the dynamics of biosurfactant production by the Bacillus amyloliquefaciens VKMA B-12464 strain, as well as to evaluate its chemical nature. During the research, the resulting biosurfactant was characterized by TLC and IR spectroscopy. It has been established that the biosurfactant produced by Bacillus amyloliquefaciens belongs to lipopeptides, and its highest yield is observed on the 7th day of cultivation.
18
Sulistyarso, Harry Budiharjo, Joko Pamungkas, Sri Rahayu Gusmarwani, Indah Widyaningsih, and Kevin Yudha Perwira. "Field Test of U-Champ Biosurfactant in Oil Spill Bioremediation." International Journal of Recent Technology and Engineering (IJRTE) 10, no. 3 (September 30, 2021): 75–78. http://dx.doi.org/10.35940/ijrte.c6428.0910321.
Full textAbstract:
One of the serious environmental problems of the oil and gas industry is handling oil spills. Oil spills can be managed by using bioremediation in the form of biosurfactants. In this research, biosurfactants called U-Champ were used which can impact in remediation of oil spills. The research conducted was testing the effectiveness of biosurfactants on Cepu Field contaminated soil in reducing TPH and heavy metals. The method is done by stirring the contaminated soil with various concentrations of biosurfactant product in an artificial tank. Based on the test results, a decline in TPH and TCLP along with the increase in U-Champ biosurfactant concentration was used. The addition of 6 Liters of biosurfactant gave the most significant reduction. Some of the heavy metals in the soil decrease, but some also have increased. As an example, it can be seen in Ba metal, the addition of U-Champ biosurfactant as much as 1.5 L (0.3% v / v) resulted in a decrease in TCLP of 11.085%. The qualitative result is evidenced by the growth of various plants in each artificial tank.
19
M.B. Muhsin, Yusra, Huda Z. Majeed, Basam Basim Mohammed, and Salih A.A. Mohammed. "Lactic Acid Bacteria Biosurfactant Role That Isolated from Human Breast Milk in Inhibit Eyes Pathogenic Bacteria." Ibn AL- Haitham Journal For Pure and Applied Science 31, no. 2 (September 12, 2018): 31. http://dx.doi.org/10.30526/31.2.1959.
Full textAbstract:
Biosurfactants have a wide-range of applications due to their unique properties like specificity, not toxicity (from LAB) and relative ease of preparation. These properties hold promise of biosurfactants to increase breast milk benefit were isolated and described into Lactobacillus plantarum, Lactobacillus fermentum ,Lactococcuslactis, and Leuconostocmesenteroides.The degree of microbial destruction of disease, which promotes the effective remediation of disease spreading.This paper presents a review of available research, methods and publications regarding Biosurfactant extraction from Lactic Acid bacteria isolated from human breast milk. 3 samples of human breast milk was provided, LAB were isolated and described, Biosurfactants recovery and surface activity were tested and extracted endo and extra cellular. In other side 26 samples from eye patients were ordered, diagnosed and their sensitivity to biosurfactant were studied. The results showed that 5 isolates of LAB from human breast milk were biosurfactant producer but L. plantarum was the more efficiency in surface activity. In other side, out of 26 eyes sample 18 were positive to pathogenic bacteria included E.coli (7), Klebseilla (5), Staphylococcus aureus(3)and S.epidermides (3).Extracellular Biosurfactanthad good effect against tested bacteria, but endocellular (extraction by normal method) had not any effect against any bacteria, whereas by solvents method were the more active against all tested bacteria. The results are promising enough to continue the quest for enhancement of inhibition growth of pathogenic bacteria with biosurfactant application (extracted extracellular by solvents) to look forward for biosurfactant as a solution of antibiotic resistance problem. In this study we concluded thatL. plantarum was the more effectiveness in biosurfactant surface activity and the extracellular biosurfactant by solvent method for extraction were better than endocellular and normal method of extraction.
20
Stella, Nnabugwu Chidmma, Ekwealor, Chito Clare, Anaukwu, Chikodili Gladys, Anakwenze Vivian Nonyelum, Mba, Anthonia Nkiru, and Ekwealor, Ikechukwu Amaechi. "Isolation and Screening of Biosurfactant-Producing Bacteria from Hydrocarbon-Contaminated Soils in Awka, Southeast, Nigeria." Microbiology Research Journal International 33, no. 10 (December 6, 2023): 9–19. http://dx.doi.org/10.9734/mrji/2023/v33i101407.
Full textAbstract:
Background: Biosurfactants are a variety of groups of active compounds produced by various types of microorganisms. Bacterial surfactants are suitable for a range of applications in the oil industry, food, agriculture, pharmaceutical, cosmetics and bioremediation. Therefore, the search for sustainable alternatives to production of chemical biosurfactant is widely being encouraged.
Aim: The present study aimed to isolate and screen bacterial species for biosurfactant production.
Methodology: Two hundred soil samples collected from hydrocarbon contaminated soils in Awka, Nigeria, were cultured on casein starch agar. The isolates were screened for biosurfactant production stability and antimicrobial activity.
Results: Primary screening on the 87 recovered isolates using drop collapse, oil spreading, haemolysis tests showed that 16 isolates (18.39%) were biosurfactant producers. Secondary screening resulted in 9 isolates with good emulsification index (E24) ranging from 42.18% to 74.07%. The culture supernatant of the isolates containing biosurfactants were stable at varying pH (2 – 12), temperature (4 - 121˚C) and salinity (2 – 20%). Most isolates have antimicrobial activity against Escherichia coli and Staphylococcus aureus. Based on oil spreading, emulsification index and antimicrobial activity, four of the isolates, ASP, AS1, DS4, DS8, were selected as most efficient in biosurfactant production. Phylogenetic analysis based on 16S rRNA genes classified ASP as Streptomyces sp, AS1 as Actinomyces sp, DS4 and DS 8 as species of Alcaligenes faecalis.
Conclusion: All the species investigated were observed to be active biosurfactant producers. The stability of the biosurfactants, and their antimicrobial nature, make them suitable candidates for use in various industrial sectors.
21
Matei, Gabi Mirela, Sorin Matei, Adrian Matei, and Elena Draghici. "Antifungal activity of a biosurfactant-producing lactic acid bacteria strain." EuroBiotech Journal 1, no. 3 (July 20, 2017): 212–16. http://dx.doi.org/10.24190/issn2564-615x/2017/03.02.
Full textAbstract:
Abstract Lactic acid bacteria are frequently utilized in food industry and they are also recognized as antimicrobial agents due to their capability to produce metabolites such as: organic acids, biosurfactants, bacteriocins, hydrogen peroxide, cyclic dipeptides, exopolysaccharides. The main goal of this paper was to present the results of the research carried out on the strain LCM2 of lactic acid bacteria isolated from brined cucumbers, for production of biosurfactants and to assess its antifungal properties. The emulsification capacity of biosurfactant was measured using kerosene as the hydrophobic substrate. The value of emulsification index E24 was 89.04% showing a high emulsification activity of the biosurfactant. The structural characterization of biosurfactant by TLC revealed its glycolipidic nature. Assay of the ionic charge established the anionic charge of the biosurfactant revealed by the presence of precipitation lines towards the cationic surfactant dodecyl-dimethyl-ammonium chloride. The biosurfactant presented antibiofilm activity with low adherence capacity, structural damages of the hyphal net, conidiophores and delays or lack of sporulation and decreased biomass accumulation in four mycotoxigenic Penicillium and Aspergillus isolates. Results of in vitro assays recommend the biosurfactant produced by the new lactic acid bacteria strain LCM2 for biotechnological purposes, as alternative antifungal agent in food industry.
22
Abdalsadiq, Nagea, Zaiton Hassan, and Mohd LANI. "CHARACTERIZATION OF THE PHYSICOCHEMICAL PROPERTIES OF THE BIOSURFACTANT PRODUCED BY L. ACIDOPHILUS AND L. PENTOSUS." EPH - International Journal of Science And Engineering 7, no. 4 (December 27, 2021): 34–42. http://dx.doi.org/10.53555/ephijse.v7i4.193.
Full textAbstract:
Biosurfactants or surface-active compounds are biodegradable, non-toxic and eco-friendly compounds released by microorganisms. Biosurfactants are amphiphilic compounds cause surface tension reduction both aqueous solutions and hydrocarbon mixtures. The main purpose of this work was to characterize biosurfactant produced by Lactobacillus strains. Identification using 16s rDNA identified the isolates as L. acidophilus for Fm1 and L. pentosus for Y1. Effect different parameters (temperature, PH and Salinity) were studied to evaluate the stability of biosurfactant after treatment. In addition, critical micelle concentration of biosurfactant, emulsification index and viscosity reduction of palm and engine oils have been studied. The results revealed that, the biosurfactant from L. acidophilus and L. pentosus maintains its emulsifications activities unaffected in the wide range of parameter's study except slightly decreasing in emulsification index values at salinity 15%. The maximum reduction in surface tension was 18.05 mN/m with minimum concentration of critical micelle concentration of 7.5 mg/ml and high decrease of palm and engine oil viscosity of 110.1 and 165.3% respectively. This study concluded that, the emulsification activity, the surface activity and the stability to heat treatment, different PH and salinity of biosurfactant of Lactobacillus strains revealed the application of the biosurfactant in food, pharmaceutical, cosmetics industries and oil recovery.
23
Gudiña, Eduardo J., José A. Teixeira, and Lígia R. Rodrigues. "Biosurfactant-Producing Lactobacilli: Screening, Production Profiles, and Effect of Medium Composition." Applied and Environmental Soil Science 2011 (2011): 1–9. http://dx.doi.org/10.1155/2011/201254.
Full textAbstract:
Biosurfactant production was screened in four lactobacilli strains. The highest biosurfactant production (excreted and cell-bound biosurfactants) was achieved withLactobacillus paracaseissp.paracaseiA20, a strain isolated from a Portuguese dairy plant, with a decrease in the surface tension of 6.4 mN m−1and 22.0 mN m−1, respectively. Biosurfactant production by this strain was evaluated under different culture broth compositions. The use of different nitrogen sources revealed that yeast extract is essential for bacterial growth, while peptone is crucial for biosurfactant synthesis. For biosurfactant production, the use of peptone and meat extract yielded a higher production when compared to the standard medium, with a surface tension reduction of 24.5 mN m−1Furthermore, experiments were also conducted in a reactor with pH and temperature control. Biomass and biosurfactant production in bioreactor was higher comparing with the experiments conducted in shake flaks. The optimization procedure adopted in the current work was found to improve the biosurfactant production and opened new perspectives for the use ofL. paracaseissp.paracaseiA20 as a promising biosurfactant-producer.
24
Zamani Beidokhti, Majid, and Daryoush Yousefi Kebria. "Evaluation of biosurfactant production by Sporosarcina halophila and its application in crude oil remediation." Environmental Health Engineering and Management 9, no. 4 (November 5, 2022): 375–79. http://dx.doi.org/10.34172/ehem.2022.40.
Full textAbstract:
Background: Biosurfactants are valuable microbial products that have significant applications in various industries. The advantages of these compounds are biodegradability, low toxicity, activity in difficult environmental conditions, and the ability to produce oil residues and compounds from the surface of seawater and soils contaminated with oil compounds. The aim of this study was to evaluate the ability of biosurfactants production by Sporosarcina halophila. Methods: For this purpose, to detect the production of biosurfactant by Sporosarcina halophila, quantitative and qualitative screening methods such as hemolysis, oil spreading test and emulsification index test were used. Finally, different concentrations of crude oil in the bacterial growth medium were used to see that this strain can decompose crude oil using biosurfactant production to continue its growth or not. Results: The results showed that this strain is able to produce biosurfactants in oil hemolysis and spreading test with emulsifying activity of more than 30%, indicating that this strain is a suitable strain for biosurfactant production. Also, this strain could grow in the presence of crude oil in its medium as only carbon resource by biosurfactant production. Conclusion: This study showed that the metabolites derived from Sporosarcina halophila strain have emulsifying properties and can be considered as a potent strain in the production of biosurfactants. Also, it was concluded that these biosurfactants are applicable for many different industrial or environmental fields such as bioremediation of crude oil from soil or water by Sporosarcina halophila strain.
25
Biktasheva, Liliya, Alexander Gordeev, Anastasia Kirichenko, Polina Kuryntseva, and Svetlana Selivanovskaya. "Screening of Microorganisms from Wastes and Identification of the Optimal Substrate for Biosurfactant Production." Microbiology Research 15, no. 1 (January 6, 2024): 152–63. http://dx.doi.org/10.3390/microbiolres15010010.
Full textAbstract:
The production of biosurfactants from organic wastes has received significant attention due to its potential cost savings. This study involved the isolation of biosurfactant-producing microorganisms from waste sources. The surfactant properties of the 37 studied isolates were assessed by reducing surface tension and their emulsifying properties, determined by the emulsification index E24. We assessed the ability of these isolated strains to produce biosurfactants using various waste substrates, namely potato peelings, waste cooking oil and sunflower cake. Our results showed that sunflower cake exhibited better growth and biosurfactant production for most of the strains studied. This highlights that sunflower cake is a potentially effective and economical substrate for the production of biosurfactants. The most effective strains allowing to achieve an emulsification index above 50% and reduce surface tension below 40 mN m−1 were Enterobacter sp. 2pp, strain 2wfo, Peribacillus sp. 1mo, Sphingomonas sp. 2mo, Ochrobactrum sp. 5mo, Shouchella sp. 6mo, Bacillus sp. 1os, Bacillus sp. 2os. Among these strains, both previously known strains as biosurfactant producers and previously unknown strains were found. Thus, we found that among representatives of the genus Sphingomonas there are effective producers of biosurfactants. The highest yield of biosurfactant on a medium with glycerol and glucose was shown by the Bacillus sp. 2os strain of 0.501 and 0.636 g L−1, respectively.
26
Abdul Rahim, Ainihayati, Wan Nurul Hakimah Wan Azmi, Noor Azlina Ibrahim, Wan Nurul Iffan Sofea Wan Mohamad Safari, and Khomaizon Abdul Kadir Pahirul Zaman. "Isolation of Potential Biosurfactant Producer from Oil Contaminated Soil and Water." Journal of Tropical Resources and Sustainable Science (JTRSS) 5, no. 3 (December 31, 2017): 140–44. http://dx.doi.org/10.47253/jtrss.v5i3.659.
Full textAbstract:
Biosurfactants are extracellular macromolecules produced by bacteria, yeast, and fungi when grown on different carbon sources. It has the ability to reduce the surface tension of a liquid, interfacial tensions between two liquids and between a liquid and a solid. This study was conducted to isolate potential biosurfactant producers from oil-contaminated soil and water. Soil and water samples were obtained from the food court area in front of Universiti Malaysia Kelantan, Jeli Campus. Isolation of biosurfactant producing bacteria was carried out on minimal salt medium (MSM) supplemented with palm oil as the sole carbon source. Five potential biosurfactant producers; WS2, WS4, WS5, SS2 and SS5 were successfully isolated and identified by 16S rRNA analysis. Isolate WS4, SS2 and SS5 showed the highest similarity to Klebsiella sp and the other two isolates, WS2 and SS5 showed the highest similarity to Pseudomonas sp. and Nanobacterium sp. respectively. While Klebsiella sp. and Pseudomonas sp. were reported as prevalent biosurfactant producer, no report is available on the production of biosurfactants by Nanobacterium sp. All isolates showed variation in biosurfactant characterization assays which are emulsification test, drop collapse test, oil spreading test, blood haemolysis and blue agar plate assay.
27
Youssef, N., D. R. Simpson, K. E. Duncan, M. J. McInerney, M. Folmsbee, T. Fincher, and R. M. Knapp. "In Situ Biosurfactant Production by Bacillus Strains Injected into a Limestone Petroleum Reservoir." Applied and Environmental Microbiology 73, no. 4 (December 15, 2006): 1239–47. http://dx.doi.org/10.1128/aem.02264-06.
Full textAbstract:
ABSTRACT Biosurfactant-mediated oil recovery may be an economic approach for recovery of significant amounts of oil entrapped in reservoirs, but evidence that biosurfactants can be produced in situ at concentrations needed to mobilize oil is lacking. We tested whether two Bacillus strains that produce lipopeptide biosurfactants can metabolize and produce their biosurfactants in an oil reservoir. Five wells that produce from the same Viola limestone formation were used. Two wells received an inoculum (a mixture of Bacillus strain RS-1 and Bacillus subtilis subsp. spizizenii NRRL B-23049) and nutrients (glucose, sodium nitrate, and trace metals), two wells received just nutrients, and one well received only formation water. Results showed in situ metabolism and biosurfactant production. The average concentration of lipopeptide biosurfactant in the produced fluids of the inoculated wells was about 90 mg/liter. This concentration is approximately nine times the minimum concentration required to mobilize entrapped oil from sandstone cores. Carbon dioxide, acetate, lactate, ethanol, and 2,3-butanediol were detected in the produced fluids of the inoculated wells. Only CO2 and ethanol were detected in the produced fluids of the nutrient-only-treated wells. Microbiological and molecular data showed that the microorganisms injected into the formation were retrieved in the produced fluids of the inoculated wells. We provide essential data for modeling microbial oil recovery processes in situ, including growth rates (0.06 � 0.01 h−1), carbon balances (107% � 34%), biosurfactant production rates (0.02 � 0.001 h−1), and biosurfactant yields (0.015 � 0.001 mol biosurfactant/mol glucose). The data demonstrate the technical feasibility of microbial processes for oil recovery.
28
Zargar, Arif Nissar, Sarthak Mishra, Manoj Kumar, and Preeti Srivastava. "Isolation and chemical characterization of the biosurfactant produced by Gordonia sp. IITR100." PLOS ONE 17, no. 4 (April 14, 2022): e0264202. http://dx.doi.org/10.1371/journal.pone.0264202.
Full textAbstract:
Biosurfactants are amphipathic molecules produced from microorganisms. There are relatively few species known where the detailed chemical characterization of biosurfactant has been reported. Here, we report isolation and chemical characterization of the biosurfactant produced by a biodesulfurizing bacterium Gordonia sp. IITR100. Biosurfactant production was determined by performing oil spreading, drop-collapse, Emulsion index (E24), and Bacterial adhesion to hydrocarbons (BATH) assay. The biosurfactant was identified as a glycolipid by LCMS and GCMS analysis. The chemical structure was further confirmed by performing FTIR and NMR of the extracted biosurfactant. The emulsion formed by the biosurfactant was found to be stable between temperatures of 4°C to 30°C, pH of 6 to 10 and salt concentrations up to 2%. It was successful in reducing the surface tension of the aqueous media from 61.06 mN/m to 36.82 mN/m. The biosurfactant produced can be used in petroleum, detergents, soaps, the food and beverage industry and the healthcare industry.
29
López-Prieto, Alejandro, Xanel Vecino, Lorena Rodríguez-López, Ana Belén Moldes, and José Manuel Cruz. "Fungistatic and Fungicidal Capacity of a Biosurfactant Extract Obtained from Corn Steep Water." Foods 9, no. 5 (May 20, 2020): 662. http://dx.doi.org/10.3390/foods9050662.
Full textAbstract:
Biosurfactants are surface-active compounds that are produced by microorganisms, which in addition to their surfactant capacity, can possess interesting antimicrobial activities that are used in their incorporation into the agrifood industry. In this work, the preservative capacity of a novel biosurfactant extract obtained from a residual stream of the corn-milling industry was evaluated against two different fungi (Aspergillus brasiliensis and Candida albicans) under different biosurfactant concentrations (0.33–0.99 mg/mL), temperatures (4–40 °C), and incubation times (5–11 days). All the assays started with the same concentration of fungi (2 × 106 CFU/mL). The results showed that temperature played an important role in the bactericidal and fungistatic effects of this biosurfactant extract. It was observed that at a low biosurfactant concentration (0.33 mg/mL) and low or high temperatures in the range tested, this biosurfactant extract possessed an important fungicidal effect (complete inhibition) on A. brasiliensis, while at intermediate temperatures, it achieved a fungistatic effect (50% of inhibition). Regarding C. albicans, it was observed that this strain was more resistant than A. brasiliens, although it was possible to achieve growth inhibitions of 76.3% at temperatures of 40 °C after 8 days of incubation with a biosurfactant concentration of 0.99 mg/mL. This work supports the possible application of biosurfactants extracted from corn steep water as preservatives and antimicrobial agents against fungal contaminations on agrifood products.
30
Lvova, Ksenia, Andrea Martínez-Arcos, Alejandro López-Prieto, Xanel Vecino, Ana Belén Moldes, and José Manuel Cruz. "Optimization of the Operational Conditions to Produce Extracellular and Cell-Bound Biosurfactants by Aneurinibacillus aneurinilyticus Using Corn Steep Liquor as a Unique Source of Nutrients." Fermentation 9, no. 4 (April 3, 2023): 351. http://dx.doi.org/10.3390/fermentation9040351.
Full textAbstract:
The relevance of this work lies in the fact that it is the first time that corn steep liquor (CSL) has been proposed as a unique source of nutrients for producing biosurfactants in a controlled fermentation and Aneurinibacillus aneurinilyticus, isolated from CSL, has been evaluated for producing extracellular and cell-bound biosurfactants in a controlled fermentation, using secondary raw materials as a source of nutrients. In the present study, A. aneurinilyticus was inoculated into the culture medium containing sterilized CSL solutions (100–400 g L−1) and incubated using different temperatures (20–60 °C) and fermentation times (8–30 days). The dependent variables under study were the concentration of extracellular biosurfactants and cell-bound biosurfactant production in terms of critical micellar concentration (CMC), as well as the C/N ratio for cell-bound biosurfactant extracts. It was observed that CSL could increase the concentration of extracellular biosurfactants produced by A. aneurinilyticus if these were fermented during 19 days at 40 °C, using 250 g L−1 of CSL; a mean value of 6 g L−1 for extracellular biosurfactants and favorable CMC concentrations enabled the detection of cell-bound biosurfactant extracts under these conditions. Hence, these conditions could be considered optimal for producing both extracellular and cell-bound biosurfactants from CSL.
31
Lamilla, Claudio, Heidi Schalchli, Gabriela Briceño, Bárbara Leiva, Pamela Donoso-Piñol, Leticia Barrientos, Vanessa A. L. Rocha, Denise M. G. Freire, and M. Cristina Diez. "A Pesticide Biopurification System: A Source of Biosurfactant-Producing Bacteria with Environmental Biotechnology Applications." Agronomy 11, no. 4 (March 25, 2021): 624. http://dx.doi.org/10.3390/agronomy11040624.
Full textAbstract:
Biosurfactants, a wide group of compounds produced by different microorganisms, generally have less toxicity and are more biodegradable than synthetic surfactants. Biosurfactant-producing bacteria can be found in contaminated environments, such as soils receiving pesticide applications constantly, or in pesticides treatment systems where microorganisms are adapted to biodegrading pesticides. Five pesticide-tolerant bacteria previously isolated from a pesticide biopurification system were evaluated as biosurfactant-producers. Pseudomonas rhodesiae C4, Rhodococcus jialingiae C8 and Pseudomonas marginalis C9 strains were positive in qualitative tests. Biosurfactant production by these strains using Bushnell-Haas medium with olive oil at 2% (w/v) was evaluated as emulsification index, oil displacement, droplet collapse test and surface tension. After 144 h, these strains showed a similar emulsification index of >55%. The two Pseudomonas (C4 and C9) strains showed lower superficial tension compared with Rhodococcus strain (C8)—34.47, 37.44 and 47.55 mN/m for strains C4, C9 and C8, respectively. The chemical characterization of the biosurfactants revealed the presence of glycolipids in P. rhodesiae (C4) and glycopeptides in P. marginalis (C9). The degradation of chlorpyrifos increased from 39.2% to 51.6% when biosurfactants produced by P.rhodesiae (C4) were added (10%) with respect to the control. Therefore, biopurification systems are a relevant source of biosurfactant-producing bacteria with environmental biotechnology applications.
32
Durval, Italo J. B., Beatriz G. Ribeiro, Jaciana S. Aguiar, Raquel D. Rufino, Attilio Converti, and Leonie A. Sarubbo. "Application of a Biosurfactant Produced by Bacillus cereus UCP 1615 from Waste Frying Oil as an Emulsifier in a Cookie Formulation." Fermentation 7, no. 3 (September 12, 2021): 189. http://dx.doi.org/10.3390/fermentation7030189.
Full textAbstract:
Biosurfactants have attracted increasing interest from the food industry due to their emulsifying, foaming and solubilizing properties. However, the industrial use of microbial biosurfactants has been hampered by the high production costs related mainly to the use of expensive substrates. The search for low-cost alternative substrates is one of the strategies adopted to overcome this problem. In the present study, a biosurfactant produced by Bacillus cereus UCP1615 by fermentation in a medium supplemented with waste frying soybean oil as a low-cost substrate was evaluated as a bioemulsifier for the production of cookies. The biosurfactant was evaluated for its emulsifying capacity against different vegetable oils, antioxidant activity and toxicity, demonstrating favorable results for use in food. In particular, it showed satisfactory antioxidant activity at the tested concentrations and no cytotoxicity to the L929 (mouse fibroblast) and Vero (monkey kidney epithelial) cell lines using the MTT assay. The biosurfactant was then added at different concentrations (0.25%, 0.5% and 1%) to a standard cookie dough formulation to evaluate the physicochemical characteristics of the product. Cookies formulated with the biosurfactant exhibited similar energy and physical characteristics to those obtained with the standard formulation but with a lower moisture content. The biosurfactant also ensured a good preservation of the cookie texture after 45 days of storage. These results suggest that the biosurfactant has a potential application as a green emulsifier in accordance with the demands of the current market for biocompatible products.
33
D. Albino, John, and Indumathi M. Nambi. "Partial Characterization of Biosurfactant Produced under Anaerobic Conditions by Pseudomonas sp ANBIOSURF-1." Advanced Materials Research 93-94 (January 2010): 623–26. http://dx.doi.org/10.4028/www.scientific.net/amr.93-94.623.
Full textAbstract:
In-situ applications such as Microbial Enhanced Oil Recovery (MEOR) and remediation of contaminated sites demand production of biosurfactants in large quantities under oxygen limiting conditions. Few microorganisms have been isolated so far which can cater such need. In this paper, the characteristics of a biosurfactant produced under complete anaerobic conditions are presented. A novel biosurfactant producing microorganism, Pseudomonas sp ANBIOSURF-1 was isolated in our laboratory, from a microbial consortium enriched from municipal sewage sludge. The microorganism utilized vegetable oils and produced biosurfactant under complete anaerobic conditions. TLC tests revealed the presence of sugar and lipid as hydrophilic and hydrophobic moieties respectively. The biosurfactant synthesized under anaerobic condition thus belonged to class of glycolipids similar to that of rhamnolipids. The biosurfactant had a very low CMC value of 52 mg/l. The biosurfactant displayed good emulsifying activity over chlorinated solvents than the petroleum derivatives. The results suggest that Pseudomonas sp ANBIOSURF-1 could potentially be used for remediation of sites contaminated with chlorinated solvents through in-situ biosurfactant production.
34
Danang Susetyo, Rizky, Endah Retnaningrum, Wahyu Wilopo, Suwarno Hadisusanto, Salamun, Ni’matuzahroh, and Fatimah. "Combination of Mollase and Glucose as Substrate for The Production of Biosurfactant by Bacillus subtilis BK7.1." BIO Web of Conferences 101 (2024): 02007. http://dx.doi.org/10.1051/bioconf/202410102007.
Full textAbstract:
Biosurfactant is a secondary metabolite which has properties and structures such as surfactants that are able to decrease surface tension water and cause microsolubilization or emulsification. Biosurfactants are active compounds that are produced at the microbial cell surface or excreted especially Bacillus. Previous research reported that B. subtilis BK7.1 had ability to form biosurfactant. In this study, B. subtilis BK7.1 produced biosurfactant using a combination of molasse and glucose as a carbon source. The purpose of study was characterized biosurfactant from B. subtilis BK7.1 by counting the emulsification index and the surface tension of supernatant, calculating (CMC) value, and examining stability of biosurfactant. B. subtilis BK7.1 could produce the biosurfactant from molasse and glucose with CMC value was about 4 g/L. Biosurfactant of B. subtilis BK7.1 could reduce the surface tension of medium from 54.68 to 49.2 mN/m, emulsify kerosene around 15.8%, had temperature stability in the range of 27⁰C to 45⁰C, and had stability at pH 6. This study showed that the use of molasses waste combined with glucose in biosurfactant production was very efficient and had potential for further applications.
35
Al-Sulaimani, H., Y. Al-Wahaibi, S. Al-Bahry, A. Elshafie, A. Al-Bemani, S. Joshi, and S. Zargari. "Optimization and Partial Characterization of Biosurfactants Produced by Bacillus Species and Their Potential for Ex-Situ Enhanced Oil Recovery." SPE Journal 16, no. 03 (June 9, 2011): 672–82. http://dx.doi.org/10.2118/129228-pa.
Full textAbstract:
Summary This study aims to test the potential of microbial enhanced oil recovery (MEOR) as an effective alternative in Omani oil fields. In this study, biosurfactants produced by Bacillus licheniformis and Bacillus subtilis strains isolated from oil-contaminated soils from different parts of the Sultanate of Oman were investigated. Eight different minimal production media using different sugars as carbon sources were tested on three of the strains to select the medium that maximized the production of biosurfactants which were indicated by the interfacial tension (IFT) reduction. All isolates were tested on their potential media to screen for the best biosurfactant producer among the available strains. It was found that Bacillus subtilis Strain W19 gave the maximum IFT reduction (46.6 mN/m to 3.28 mN/m) in 16 hours of incubation when grown in a minimal medium containing glucose. The yield of the biosurfactant produced by B. subtilis W19 was 2.5g/L. Critical micelle concentration (CMC) was measured to be 0.4 g/L. The biosurfactant was partially characterized by FT-IR analysis, in which the peaks obtained imply the presence of aliphatic hydrocarbons as well as a peptide-like moiety in the biosurfactant. The cell-free biosurfactant broth produced from Strain W19 was found to be stable over a pH range from 6 to 10 and was most effective at 7. It also showed no loss in surface activity when subjected to various temperatures (60, 80, and 120°C). The biosurfactant also retained almost 60% of its activity even in a high-saline environment up to 20% NaCl (w/v). Further studies were conducted to test the interaction of biosurfactant produced by B. subtilis W19 with porous media in coreflooding experiments as a tertiary-recovery stage. The results showed high potential of using this bacterium during ex-situ MEOR applications in which a total of 23% of residual oil was produced after biosurfactant and concentrated-biosurfactant injection.
36
Khamis, Nurazeerah, Ainihayati Abdul Rahim, Noor Azlina Ibrahim, and Khomaizon Abdul Kadir Pahirul Zaman. "MICROBIAL SURFACTANTS: CLASSIFICATIONS, PROPERTIES, RECOVERY, AND APPLICATIONS." Jurnal Teknologi 84, no. 2 (January 27, 2022): 35–45. http://dx.doi.org/10.11113/jurnalteknologi.v84.16477.
Full textAbstract:
Surface-active agents or surfactants are amphiphilic compounds derived naturally or chemically synthesized. Natural surfactants or so-called biosurfactants can be obtained from bacteria, yeasts, and fungi as well as plants. Due to their hydrophilic and hydrophobic molecules, biosurfactants offer the ability to reduce surface or interfacial tension and create microemulsion between aqueous and organic phases. Many studies have suggested biosurfactants as a better substitution to chemical surfactants since they are more environment friendly, biocompatible, and has lower toxicity. This review article is provided to introduce some general information regarding microbial-derived biosurfactants including five main classes of biosurfactants and several distinctive properties that confer them as promising surface-active agents. Due to their diverse molecular structures, biosurfactants have been applied in various applications. Thus, several common biosurfactant recovery methods are discussed and examples of biosurfactant applications in hydrocarbon removal as well as in industrial processing are highlighted.
37
Edosa, Tariku Tesfaye, Yong Hun Jo, Maryam Keshavarz, In Seon Kim, and Yeon Soo Han. "Biosurfactants Induce Antimicrobial Peptide Production through the Activation of TmSpatzles in Tenebrio molitor." International Journal of Molecular Sciences 21, no. 17 (August 24, 2020): 6090. http://dx.doi.org/10.3390/ijms21176090.
Full textAbstract:
Biosurfactant immunomodulatory activities in mammals, nematodes, and plants have been investigated. However, the immune activation property of biosurfactants in insects has not been reported. Therefore, here, we studied the defense response triggered by lipopeptides (fengycin and iturin A), glycolipids (rhamnolipid), and cyclic polypeptides (bacitracin) in the coleopteran insect, mealworm Tenebrio molitor. The in vitro antimicrobial activities against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and fungi (Candida albicans) were assessed by mixing these pathogens with the hemolymph of biosurfactant-immune-activated larvae. E. coli growth was remarkably inhibited by this hemolymph. The antimicrobial peptide (AMP) induction results also revealed that all biosurfactants tested induced several AMPs, exclusively in hemocytes. The survivability analysis of T. molitor larvae challenged by E. coli (106 CFU/µL) at 24 h post biosurfactant-immune activation showed that fengycin, iturin A, and rhamnopid significantly increased survivability against E. coli. Biosurfactant-induced TmSpatzles activation was also monitored, and the results showed that TmSpz3 and TmSpz-like were upregulated in the hemocytes of iturin A-injected larvae, while TmSpz4 and TmSpz6 were upregulated in the fat bodies of the fengycin-, iturin A-, and rhamnolipid-injected larvae. Overall, these results suggest that lipopeptide and glycolipid biosurfactants induce the expression of AMPs in T. molitor via the activation of spätzle genes, thereby increasing the survivability of T. molitor against E. coli.
38
Bharali, Pranjal, Salam Pradeep Singh, Yasir Bashir, Nipu Dutta, Bolin Kumar Konwar, and Chingakham Brajakishor Singh. "Characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria: potential application in bioremediation." Nova Biotechnologica et Chimica 17, no. 2 (December 1, 2018): 103–14. http://dx.doi.org/10.2478/nbec-2018-0011.
Full textAbstract:
Abstract Petroleum and hydrocarbons contamination can be remediated by physical, chemical or biological methods. Among these, in situ bioremediation is considered to be environmentally friendly because it restores the soil structure, requires less energy input and involves the notable removal after degradation of biosurfactant. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques. The results clearly demonstrate the capability of utilizing hydrocarbon and biosurfactant produced by the bacterial strains. 16S rDNA sequencing followed by BLAST analysis revealed their similarity to Pseudomonas aeruginosa. The physico-chemical characterization of the biosurfactants revealed significant surface properties with stability at extreme temperature conditions (up to 121˚C), pH (5 - 8) and salinity (up to 4 %). Further, the mass spectrometry confirmed predominance of di-rhamnolipids in biosurfactant mixtures. The biosurfactants were found to be efficient in the removal of crude oil from the contaminated sand suggesting its applicability in bioremediation technology. Further, improved discharge of crude oil at elevated temperatures also confirms their thermo-stability which, could be exploited in microbial enhanced oil recovery processes. Thus, the applications of biosurfactants produced by the indigenous hydrocarbonoclastic strains appeared to be advantageous for bioremediation of petroleum-contaminated environments.
39
Verma, Rupa, Mukul Agrawal, Abhay Dundung, and Ladly Rani. "Screening of Biosurfactant Production in Bacteria Isolated from Oil and Pesticide Contaminated Soil of Ranchi District." Journal of Scientific Research 66, no. 04 (2022): 29–34. http://dx.doi.org/10.37398/jsr.2022.660405.
Full textAbstract:
Biosurfactants, the surface-active compounds are produced by a few microorganisms. As such biosurfactants are superior to synthetic surfactants in terms of cost of production and industrial application. Present study deals with the isolation, characterization, screening, and extraction of biosurfactant producing bacteria from motor oil dumped and pesticide contaminated agricultural fields of Ranchi District, Jharkhand, India. Mineral salt medium (MSM) supplemented with hydrocarbon was used for the enrichment of putative biosurfactant producers. Out of ten isolates, five isolates (F1 to F5) showed growth on hydrocarbon supplemented plate suggesting its use as main carbon source. Three isolates namely F1, F4 and F5 were from the waste oil contaminated soil and remaining two (F2 and F3) from pesticide contaminated site. Based on traditional microbiological methods, characterization and identification were made which showed that F1, F4 and F5 isolates belong to Staphylococcus aureus and F2 and F3 to Bacillus subtilis species. Biosurfactant production was tested by hemolysis, emulsification index (E24) and drop collapsing tests, results showed positive test for all the assay suggesting the potential of biosurfactant production by all the five isolates. Among all the five isolates, F1 showed maximum emulsification index (44.44%) followed by F4 (35%) both belonging to strains of Staphylococcus aureus. The remaining isolates F2, F3 and F5 also showed appreciable level of E24 (24-28%). Biosurfactants produced by all the five isolates were extracted using solvents, the dry weight showed close correlation with E24. Further work is needed to confirm the identity of all the isolates using 16S rRNA sequencing and chemical characteristics of biosurfactants employing standard analytical techniques.
40
Azman, Nur Raudhah, Umi Aisah Asli, and Amnani Shamjuddin. "Elucidation of Kinetic Studies in Biosurfactant Fermentative Production and Their Behaviour: A Mini Review." Journal of Bioprocessing and Biomass Technology 2, no. 2 (December 29, 2023): 45–53. http://dx.doi.org/10.11113/bioprocessing.v2n2.38.
Full textAbstract:
Bioprocess engineering, which include kinetic behaviour, is a fundamental form of developing effective product performance and functionality. Kinetic studies are one of the most important steps in any bioprocess and bioproduct development to elucidate the production phase and product behaviour. The lack of comprehensive understanding and limited knowledge of the kinetic behaviour of biosurfactant production, especially in the complex fermentation process involving microorganisms, substrate, and product formation, is major challenge and hinder the development of optimized biosurfactant in the industrial scale. Kinetic studies in the field of biosurfactants need to be emphasized to better understand the mechanisms of biosurfactant formation through biomass growth and substrate consumption. In this mini-review, various kinetic models used in biosurfactant work were critically discussed. The objective of this study is to analyze the kinetic studies of biomass growth, biosurfactant formation and substrate consumption focusing on the biosurfactant itself. This review revealed the various kinetic models used in fermentative biosurfactant production and how the different models were used and interpreted based on different substrates and biosurfactant-producing microorganisms. A key feature of this review is its focus on the biosurfactant, which provides valuable insights into the factors that maximize productivity and improve scale-up. Based on previous works, the logistic model is preferred to represent biomass growth, biosurfactant formation and substrate consumption due to its simplicity and rationale. Throughout this study, the kinetic profile of the biosurfactant able to be established and serve as a foundation for the prediction of the biosurfactant behaviour.
41
Sharuddin, Siti Shilatul Najwa, Siti Rozaimah Sheikh Abdullah, Hassimi Abu Hasan, Ahmad Razi Othman, and Israa Abdulwahab Al-Baldawi. "Enhanced Removal of Hydrocarbons from Crude Oil Sludge through Phytoremediation with Biosurfactant-producing Rhizobacteria." IOP Conference Series: Earth and Environmental Science 1307, no. 1 (February 1, 2024): 012009. http://dx.doi.org/10.1088/1755-1315/1307/1/012009.
Full textAbstract:
Abstract Discharge of crude oil (or its products) during the extracting, refining, and transporting into the environment have caused serious environmental distress due to their highly hydrophobic resistance, and persistence in nature and very difficult to be remediated from the environment. Therefore, an environmentally conscious approach to enhance the bioavailability (or solubility) of petroleum hydrocarbon pollutants in soil involves the utilization of biosurfactants. Biosurfactants play a crucial role in enhancing the desorption and solubilization of petroleum hydrocarbons, facilitating their assimilation by microorganisms. This research investigated the application of biosurfactant supplementation derived and purified from rhizobacteria of Scirpus grossus, which are capable of producing biosurfactants and degrading hydrocarbons, in the context of phytoremediation. The crude oil sludge used in this study was obtained from an industrial area containing 56,600±3;900 mg/kg of total petroleum hydrocarbon (TPH). The crude oil sludge was inoculated with biosurfactant, sodium dodecyl sulfate (SDS) as commercial surfactant and only with the presence of S. grossus in the vegetated tanks and monitored for 90 days in a greenhouse. The results indicated that the growth of S. grossus with the addition of biosurfactant was improved and new saplings were produced. After a 90-day exposure period, the removal efficiency of TPH from the soil demonstrated significant increases, reaching 90.3%, 84.1%, and 73.7% when treated with biosurfactant+S. grossus, SDS+S. grossus, and S. grossus only respectively. These percentages were notably higher compared to the non-planted control crates (CC) where the removal efficiency was only 17.9%. These results provide evidence that the introduction of biosurfactant through inoculation can elevate the bioavailability of organic pollutants, consequently augmenting their microbial degradation in the soil.
42
Almeida, Darne Germano, Rita de Cássia Freire Soares da Silva, Hugo Morais Meira, Pedro Pinto Ferreira Brasileiro, Elias José Silva, Juliana Moura Luna, Raquel Diniz Rufino, and Leonie Asfora Sarubbo. "Production, Characterization and Commercial Formulation of a Biosurfactant from Candida tropicalis UCP0996 and Its Application in Decontamination of Petroleum Pollutants." Processes 9, no. 5 (May 18, 2021): 885. http://dx.doi.org/10.3390/pr9050885.
Full textAbstract:
Contamination by oil and its derivatives causes serious damage to the environment, motivating the development of innovative technologies for the removal of these contaminants, such as the use of biosurfactants. In the present study, the biosurfactant from Candida tropicalis UCP0996 produced in the low cost-medium formulated with molasses, residual frying oil, and corn steep liquor, was characterized and its toxicity, formulation, and application in removal and biodegradation of oil were investigated. The surface tension of the medium was reduced to 30.4 mN/m, yielding 4.11 g/L of isolated biosurfactant after 120 h. Tests under extreme environmental conditions indicated the stability of the biosurfactant. Chemical characterization by thin layer chromatography (TLC), Fourier transform infrared spectroscopy (FTIR), nuclear magnetic resonance (1H NMR), and gas chromatography and mass spectroscopy (CG-MS) revealed the glycolipidic nature of the biosurfactant. The isolated biosurfactant showed no toxicity against the microcrustacean Artemia salina, while the properties of the formulated biosurfactant remained stable during 120 days of storage. The biosurfactant removed 66.18% of motor oil adsorbed in marine stones and dispersed 70.95% of oil in seawater. The biosurfactant was also able to increase by 70% the degradation of motor oil by seawater indigenous microorganisms, showing great potential to be applied as a commercial additive in the bioremediation of oil spills.
43
Moldes, A. B., R. Paradelo, X. Vecino, J. M. Cruz, E. Gudiña, L. Rodrigues, J. A. Teixeira, J. M. Domínguez, and M. T. Barral. "Partial Characterization of Biosurfactant fromLactobacillus pentosusand Comparison with Sodium Dodecyl Sulphate for the Bioremediation of Hydrocarbon Contaminated Soil." BioMed Research International 2013 (2013): 1–6. http://dx.doi.org/10.1155/2013/961842.
Full textAbstract:
The capability of a cell bound biosurfactant produced byLactobacillus pentosus, to accelerate the bioremediation of a hydrocarbon-contaminated soil, was compared with a synthetic anionic surfactant (sodium dodecyl sulphate SDS-). The biosurfactant produced by the bacteria was analyzed by Fourier transform infrared spectroscopy (FTIR) that clearly indicates the presence of OH and NH groups, C=O stretching of carbonyl groups and NH nebding (peptide linkage), as well as CH2–CH3and C–O stretching, with similar FTIR spectra than other biosurfactants obtained from lactic acid bacteria. After the characterization of biosurfactant by FTIR, soil contaminated with 7,000 mg Kg−1of octane was treated with biosurfactant fromL. pentosusor SDS. Treatment of soil for 15 days with the biosurfactant produced byL. pentosusled to a 65.1% reduction in the hydrocarbon concentration, whereas SDS reduced the octane concentration to 37.2% compared with a 2.2% reduction in the soil contaminated with octane in absence of biosurfactant used as control. Besides, after 30 days of incubation soil with SDS or biosurfactant gave percentages of bioremediation around 90% in both cases. Thus, it can be concluded that biosurfactant produced byL. pentosusaccelerates the bioremediation of octane-contaminated soil by improving the solubilisation of octane in the water phase of soil, achieving even better results than those reached with SDS after 15-day treatment.
44
Suryanti, Venty, Sri Hastuti, and Desi Suci Handayani. "BIOSYNTHESIS OF BIOSURFACTANT BY PSEUDOMONAS AERUGINOSA USING CASSAVA FLOUR INDUSTRIAL WASTEWATER AS MEDIA." ALCHEMY Jurnal Penelitian Kimia 10, no. 1 (May 24, 2018): 22. http://dx.doi.org/10.20961/alchemy.10.1.12.22-30.
Full textAbstract:
<span>Biosynthesis of biosurfactant by </span><em>Pseudomonas </em><em>aeruginosa</em><span> have been prepared using cassava flour industrial wastewater (</span><em>manipueira</em><span>) as medium. The optimum condition of the biosurfactans biosynthesis was obtained using media containing nutrient broth and manipueira without centrifugation (NBM) with 4 days fermentation. UV-Vis and FT-IR spectra indicated that the biosurfactant was a rhamnolipid containing hydroxyl, ester, carbocylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 576 mg/L and surface tension value of 0.045 N/m. The biosurfactant was able to decrease the interface tension and form emulsion with benzene, toluene, gasoline and palm oil. This biosurfactant showed w/o emulsion system.</span>
45
Suryanti, Venty, Sri Hastuti, and Desi Suci Handayani. "BIOSYNTHESIS OF BIOSURFACTANT BY PSEUDOMONAS AERUGINOSA USING CASSAVA FLOUR INDUSTRIAL WASTEWATER AS MEDIA." ALCHEMY Jurnal Penelitian Kimia 10, no. 1 (September 27, 2016): 22. http://dx.doi.org/10.20961/alchemy.v10i1.12.
Full textAbstract:
<span>Biosynthesis of biosurfactant by </span><em>Pseudomonas </em><em>aeruginosa</em><span> have been prepared using cassava flour industrial wastewater (</span><em>manipueira</em><span>) as medium. The optimum condition of the biosurfactans biosynthesis was obtained using media containing nutrient broth and manipueira without centrifugation (NBM) with 4 days fermentation. UV-Vis and FT-IR spectra indicated that the biosurfactant was a rhamnolipid containing hydroxyl, ester, carbocylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 576 mg/L and surface tension value of 0.045 N/m. The biosurfactant was able to decrease the interface tension and form emulsion with benzene, toluene, gasoline and palm oil. This biosurfactant showed w/o emulsion system.</span>
46
Bicca, Flávio Correa, Leonardo Colombo Fleck, and Marco Antônio Záchia Ayub. "Production of biosurfactant by hydrocarbon degrading Rhodococcus ruber and Rhodococcus erythropolis." Revista de Microbiologia 30, no. 3 (July 1999): 231–36. http://dx.doi.org/10.1590/s0001-37141999000300008.
Full textAbstract:
There is world wide concern about the liberation of hydrocarbons in the environment, both from industrial activities and from accidental spills of oil and oilrelated compounds. Biosurfactants, which are natural emulsifiers of hydrocarbons, are produced by some bacteria, fungi and yeast. They are polymers, totally or partially extracellular, with an amphipathyc structure, which allows them to form micelles that accumulate at the interface between liquids of different polarities such as water and oil. This process is based upon the ability of biosurfactants to reduce surface tension, blocking the formation of hydrogen bridges and certain hydrophilic and hydrophobic interactions. The ability of biosurfactant production by five strains of Rhodococcus isolated from oil prospecting sites was evaluated. Surface tension measurement and emulsifying index were used to quantify biosurfactant production. The influence of environmental conditions was also investigated - pH, temperature, medium composition, and type of carbon source - on cell growth and biosurfactant production. Strain AC 239 was shown to be a potential producer, attaining 63% of emulsifying index for a Diesel-water binary system. It could be used, either directly on oil spills in contained environments, or for the biotechnological production of biosurfactant.
47
A. Alimeer, Dijlah, Nibras N. Mahmood, and M. F. Al-Halbosiy. "Extraction and purification of biosurfactant by locally isolated Enterococcus faecium bacteria and study of its antimicrobial activity." Journal of Global Innovations in Agricultural Sciences 11, no. 4 (December 22, 2023): 543–48. http://dx.doi.org/10.22194/jgias/23.1102.
Full textAbstract:
In this research, the biosurfactant production by Enterococcus faecium isolated from Raw cow's milk was studied when growing in the MRS medium containing Lactose as a carbon source without Tween-80. E.faecium supernatant exhibited emulsion action of 60% towards kerosene oil as oil in water, while the surface activity was 34 mm. Biosurfactants with surface tension activity properties were extracted and partially purified from E. faecium by cold acetone 3:1, The chemistry structure of the biosurfactant was analyzed, which indicated that it is a glycol-lipo-peptide. Biosurfactant that purified from E. faecium showed antibacterial activity by reducing growth of several pathogenic G-ve and G+ve bacteria, including aureus, Staphylococcus epidermidis, Streptococcus sp., Staphylococcus Escherichia coli, Klebsiella sp., Pseudomonas aeroginosa, Acinetobacter sp. and Serratia sp. The inhibitory activity of biosurfactant against pathogenic bacteria was studied by using agar diffusion method, where the biosurfactant had higher inhibitor activity against E. coli, where highest diameter of inhibition zone was (23mm). At all concentrations examined, a biosurfactant derived from E. faecium considerably decreased bacterial adhesion, albeit to a lower amount. Keyword: Enterococcus faecium, LAB, Biosurfactant, Antibacterial and Antiadhesive activity.
48
Adnan, Mohd, Arif Jamal Siddiqui, Walid Sabri Hamadou, Syed Amir Ashraf, Md Imtaiyaz Hassan, Mejdi Snoussi, Riadh Badraoui, et al. "Functional and Structural Characterization of Pediococcus pentosaceus-Derived Biosurfactant and Its Biomedical Potential against Bacterial Adhesion, Quorum Sensing, and Biofilm Formation." Antibiotics 10, no. 11 (November 9, 2021): 1371. http://dx.doi.org/10.3390/antibiotics10111371.
Full textAbstract:
Biosurfactants are surface-active molecules of microbial origin and alternatives to synthetic surfactants with various applications. Due to their environmental-friendliness, biocompatibility, biodegradability, effectiveness to work under various environmental conditions, and non-toxic nature, they have been recently recognized as potential agents with therapeutic and commercial importance. The biosurfactant produced by various probiotic lactic acid bacteria (LAB) has enormous applications in different fields. Thus, in vitro assessment of biofilm development prevention or disruption by natural biosurfactants derived from probiotic LAB is a plausible approach that can lead to the discovery of novel antimicrobials. Primarily, this study aims to isolate, screen, and characterize the functional and biomedical potential of biosurfactant synthesized by probiotic LAB Pediococcus pentosaceus (P. pentosaceus). Characterization consists of the assessment of critical micelle concentration (CMC), reduction in surface tension, and emulsification index (% EI24). Evaluation of antibacterial, antibiofilm, anti-QS, and anti-adhesive activities of cell-bound biosurfactants were carried out against different human pathogenic bacteria (B. subtilis, P. aeruginosa, S. aureus, and E. coli). Moreover, bacterial cell damage, viability of cells within the biofilm, and exopolysaccharide (EPS) production were also evaluated. As a result, P. pentosaceus was found to produce 4.75 ± 0.17 g/L biosurfactant, which displayed a CMC of 2.4 ± 0.68 g/L and reduced the surface tension from 71.11 ± 1.12 mN/m to 38.18 ± 0.58 mN/m. P. pentosaceus cells bound to the crude biosurfactant were found to be effective against all tested bacterial pathogens. It exhibited an anti-adhesion ability and impeded the architecture of the biofilm matrix by affecting the viability and integrity of bacterial cells within biofilms and reducing the total EPS content. Furthermore, the crude biosurfactant derived from P. pentosaceus was structurally characterized as a lipoprotein by GC-MS analysis, which confirms the presence of lipids and proteins. Thus, our findings represent the potent anti-adhesion and antibiofilm potential of P. pentosaceus crude biosurfactant for the first time, which may be explored further as an alternative to antibiotics or chemically synthesized toxic antibiofilm agents.
49
Csutak, Ortansa Elisabeta, Nicoleta-Oana Nicula, Eduard-Marius Lungulescu, Virgil Marinescu, and Viorica Maria Corbu. "Yarrowia lipolytica CMGB32 Biosurfactants Produced Using n-Hexadecane: Developing Strategies for Environmental Remediation." Applied Sciences 14, no. 7 (April 4, 2024): 3048. http://dx.doi.org/10.3390/app14073048.
Full textAbstract:
The yeast Yarrowia lipolytica degrades petroleum compounds, including alkanes, via the monoterminal oxidation pathway, the hydrophobic carbon substrate assimilation is mediated by biosurfactants, and extracellular amphiphilic molecules are produced by the yeast cell. This study focuses on the ability of the strain Y. lipolytica CMGB32 to degrade n-hexadecane by producing biosurfactants with high potential for bioremediation. The hydrocarbon-degrading potential of the yeast strain was observed via a 2,6-dichlorophenolindophenol (DCPIP) test in Bushnell–Hass medium with 1% n-hexadecane, and cell hydrophobicity was expressed as microbial adhesion to hydrocarbons (MATH). Biosurfactant production on yeast peptone (YP) with 1% n-hexadecane was estimated after 72 h using the emulsification index (E24%) against toluene. Crude biosurfactant (cell-free broth) stability tests were performed at different temperatures (4 °C, 70 °C) and NaCl concentrations (2–10%). The effects of a biosurfactant on synthetic wastewater remediation comprised the growth curves (OD measurements) of natural heavy metal degrader Rhodotorula mucilaginosa, determination of nutrients (spectrophotometrically), physico-chemical parameters, and removal capacity of lead and cadmium ions (via inductively coupled plasma mass spectrometry—ICP-MS). The antimicrobial and anti-adherence activities of 20 mg/mL and 40 mg/mL of the biosurfactant against pathogenic Candida krusei strains involved growth observations and the crystal violet microtiter method. The DCPIP decolorization occurred after six days, corresponding to the maximum growth phase of the Y. lipolytica culture. After 72 h, the cells presented high hydrophobicity (82.61% MATH) and stable biosurfactant production (E24% 47%). The crude biosurfactant (5%) increased the growth of R. mucilaginosa strains cultivated on synthetic wastewater cultures contaminated with Pb2+ and Cd2+, increased the conductivity and COD (86%) of the samples, and determined Pb2+ (66%) and Cd2+ (42%) ions reduction. The concentrated biosurfactant inhibited C. krusei growth (70%) and biofilm adherence. In conclusion, Y. lipolytica CMGB32 shows important potential for development of biosurfactant-based technologies for the remediation of heavy-metal- and emerging pathogen-contaminated wastewaters.
50
Ibrahim Ewida, Ayman Youssef, and Walaa Salah El-din Mohamed. "Isolation and Characterization of Biosurfactant Producing Bacteria from Oil-Contaminated Water." Biosciences, Biotechnology Research Asia 16, no. 04 (December 20, 2019): 833–41. http://dx.doi.org/10.13005/bbra/2801.
Full textAbstract:
ABSTRACT: Biosurfactants are chemical compounds produced by some microorganisms to initiate oil biodegradation. They have been applied generously in many industries. The present work aimed to isolate and identify a new bacterial strain, of water habitat, capable of producing biosurfactant. So, water samples were collected from three different water environments including river Nile at Alkanater city, Qalyubia governorate; was representing clear raw water. River Nile at ship settlement station, Imbaba city, Giza governorate; was representing oil- contaminated water, where there were some oil spills from ship fixation. Rahawy drain, Giza governorate; was representing highly polluted wastewater. The bacterial community of each water environment was isolated and inventoried, then screened for biosurfactant production by blood hemolysis, oil spreading technique, drop collapse assay, foaming activity and emulsification activity. Bacterial strains isolated from the oil-contaminated environment showed high potential for biosurfactant production, and the best biosurfactant producing isolate was identified by 16S rRNA technique as Pseudomonas protegens, and the produced biosurfactant was belong to rhamnolipid group.