To see the other types of publications on this topic, follow the link: Biosurfactants.
Journal articles on the topic 'Biosurfactants'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Biosurfactants.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Liepins, Janis, Karina Balina, Raimonda Soloha, Ieva Berzina, Liva Kristiana Lukasa, and Elina Dace. "Glycolipid Biosurfactant Production from Waste Cooking Oils by Yeast: Review of Substrates, Producers and Products." Fermentation 7, no. 3 (July 29, 2021): 136. http://dx.doi.org/10.3390/fermentation7030136.
Full textAbstract:
Biosurfactants are a microbially synthesized alternative to synthetic surfactants, one of the most important bulk chemicals. Some yeast species are proven to be exceptional biosurfactant producers, while others are emerging producers. A set of factors affects the type, amount, and properties of the biosurfactant produced, as well as the environmental impact and costs of biosurfactant’s production. Exploring waste cooking oil as a substrate for biosurfactants’ production serves as an effective cost-cutting strategy, yet it has some limitations. This review explores the existing knowledge on utilizing waste cooking oil as a feedstock to produce glycolipid biosurfactants by yeast. The review focuses specifically on the differences created by using raw cooking oil or waste cooking oil as the substrate on the ability of various yeast species to synthesize sophorolipids, rhamnolipids, mannosylerythritol lipids, and other glycolipids and the substrate’s impact on the composition, properties, and limitations in the application of biosurfactants.
2
Gunjal, Aparna. "Biosurfactants from renewable sources - A review." Nepal Journal of Environmental Science 10, no. 2 (December 31, 2022): 15–23. http://dx.doi.org/10.3126/njes.v10i2.48538.
Full textAbstract:
Biosurfactants have wide applications in pharmaceutical, agriculture and food industries. The research area of biosurfactants is gaining immense attention. The review mentions here the advantages and various substrates used for biosurfactants production. The pre-treatment of substrates for biosurfactants production is also focused. The production of biosurfactants by solid state fermentation is also described. The renewable substrates, yield and microorganisms used for biosurfactant production are also taken into consideration. The screening methods for biosurfactant are also described. The use of renewable sources for biosurfactant production is specially focused in the review. This will be very eco-friendly, easy and economical. More studies need to BE done on large-scale production of biosurfactants using genetically engineered microorganisms.
3
Brinda, C. M., R. Ragunathan R. Ragunathan, and Jesteena Johney. "Diversity and Distribution of Potential Biosurfactant Producing Bacillus Sp MN 243657, GC-MS Analysis and its Antimicrobial Study." Biosciences Biotechnology Research Asia 20, no. 1 (March 30, 2023): 271–91. http://dx.doi.org/10.13005/bbra/3088.
Full textAbstract:
Biosurfactants are microbially produced surface-active compounds. They are amphiphilic molecules with hydrophilic and hydrophobic regions. The demand for biosurfactants has been exponentially growing as they are nontoxic and biodegradable. They have different applications in several industrial sectors. The objective of this study was to isolate and characterize the native bacteria which produce biosurfactants from oil contaminated soil of different places in Kerala and Tamil Nadu, India. The soil samples were collected from petrol pumps and workshops where the soil is contaminated with petrol, diesel and oil. The bacteria were isolated from contaminated soil samples and confirmed as Bacillus sp.The cultures were screened for biosurfactant production by different screening techniques such as blood hemolysis, oil spreading assay, emulsification ability assay, bacterial adherence to hydrocarbons activity. The potential biosurfactant producing culture was selected and identified using molecular techniques and submitted to NCBI Gene Bank (MN 243657 – Bacillus sp). The selected bacterial culture was used for biosurfactant production and these were characterized by UV, TLC, FTIR and GC -MS analysis. The derived biosurfactant's Rf value was 0.68 as determined by a TLC chromatogram. In a UV-visible spectroscopy study, the isolated biosurfactant displayed a highest peak at 415 nm. According to FTIR analysis, the isolated biosurfactant displayed an intense peak at 3340 cm -1. The large peaks of the biosurfactant were observed at various retention times of 12.75, 10.22, 4.98, and 3.87, respectively, after GC-MS analysis. Antibacterial and antifungal activity of the biosurfactant was identified against pathogenic bacteria such as P.aeruginosa, E. coli, K. pneumoniae, S. aureus and fungi Aspergillus niger, Aspergillus terreus, Aspergillus flavus.
4
IBRAHIM LAWAL, AMINU YUSUF FARDAMI, SULEIMAN BELLO, AISHA HABIBU, and ZAINAB MUHAMMAD SANUSI. "The Potentials of Biosurfactants as Anti-Inflammatory and Anti-Viral Agents Against Covid-19: A Mini Review." UMYU Scientifica 1, no. 2 (December 30, 2022): 156–62. http://dx.doi.org/10.56919/usci.1222.019.
Full textAbstract:
Infection due to strain of severe acute respiratory syndrome coronavirus 2 (SARS COV2) has grown to be of global public health significance. Biotechnology uses living organisms such as microbes to produce metabolites like biosurfactants. Biosurfactants are ampiphilic surface active biomolecules that were proven to have therapeutic function against some groups of microbes including viruses. They also have anti-inflammatory potential through their interaction with viral membranes and macromolecules to decrease cytosolic phospholipase A2, which is the beginning of an anti-inflammatory response, and are recognized structurally by toll-like receptors (TLR-2), which are released when neutrophils are stimulated. They can also play vital role in aiding the human body to have inflammatory response. The functional groups of biosurfactants interact with the viruses membrane structure. Some groups of biosurfactants cause physiochemical processes that render viruses inactive. Therefore it can generally be understood that biosurfactants destroy the virus's envelope and the viral membrane's structures. The principle behind biosurfactant’s anti viral property is due to the hydrophilic properties that are within the acetyl groups. Additionally, the hydrophobic properties of biosurfactant are also important in making it to have antiviral activity. These activities of biosurfactants against viruses make it to be potential anti-inflammatory and anti-viral agents against Covid-19. Therefore this paper is aimed to produce a mini review on the anti-inflammatory and anti-viral potential against Covid-19. And the review also highlights some of the desirable properties and benefits of biosurfactants as anti-corona viruses.
5
Amraini, Said Zul, Sri Rezeki Muria, Bahruddin Bahruddin, Irdoni HS, Ulfa Dwi Artha, and Reno Susanto. "Biosurfactant Production from Pseudomonas aeruginosa ATCC27853 with Carbon Source from Crude Palm Oil for Oil Recovery." Indo. J. Chem. Res. 10, no. 1 (May 31, 2022): 47–52. http://dx.doi.org/10.30598/ijcr.2022.10-sai.
Full textAbstract:
Biosurfactants are surfactants that are synthesized by microorganisms using organic materials and have biodegradable properties, making them environmentally friendly. One of the applications of biosurfactants in the recovery of petroleum. This study aims to determine the type of biosurfactant produced by Pseudomonas aeruginosa bacteria using crude palm oil as the main carbon source, to determine the effect of variations in pH and CPO concentration on surface tension reduction and emulsification, and compare the best biosurfactant with surfactant synthesis. The production of biosurfactants has 3 stages, namely the bacterial preparation, the biosurfactant production, and the analysis in the form of surface tension, emulsification, crude oil removal, and FTIR. The best biosurfactant was obtained at pH 7 and a carbon source concentration of 3% v/v with surface tension and emulsification values of 42.49 mN/m and 58%, respectively. The pH value and CPO concentration can affect the growth in the biosurfactant production process, thus also affecting the surface tension and emulsification values. The biosurfactants obtained were rhamnolipid biosurfactants. The biosurfactants produced in this study have lower crude oil recovery capabilities than synthetic surfactants with crude oil removal values of 57.78% and 79.34%, respectively.
6
Khamis, Nurazeerah, Ainihayati Abdul Rahim, Noor Azlina Ibrahim, and Khomaizon Abdul Kadir Pahirul Zaman. "MICROBIAL SURFACTANTS: CLASSIFICATIONS, PROPERTIES, RECOVERY, AND APPLICATIONS." Jurnal Teknologi 84, no. 2 (January 27, 2022): 35–45. http://dx.doi.org/10.11113/jurnalteknologi.v84.16477.
Full textAbstract:
Surface-active agents or surfactants are amphiphilic compounds derived naturally or chemically synthesized. Natural surfactants or so-called biosurfactants can be obtained from bacteria, yeasts, and fungi as well as plants. Due to their hydrophilic and hydrophobic molecules, biosurfactants offer the ability to reduce surface or interfacial tension and create microemulsion between aqueous and organic phases. Many studies have suggested biosurfactants as a better substitution to chemical surfactants since they are more environment friendly, biocompatible, and has lower toxicity. This review article is provided to introduce some general information regarding microbial-derived biosurfactants including five main classes of biosurfactants and several distinctive properties that confer them as promising surface-active agents. Due to their diverse molecular structures, biosurfactants have been applied in various applications. Thus, several common biosurfactant recovery methods are discussed and examples of biosurfactant applications in hydrocarbon removal as well as in industrial processing are highlighted.
7
Lvova, Ksenia, Andrea Martínez-Arcos, Alejandro López-Prieto, Xanel Vecino, Ana Belén Moldes, and José Manuel Cruz. "Optimization of the Operational Conditions to Produce Extracellular and Cell-Bound Biosurfactants by Aneurinibacillus aneurinilyticus Using Corn Steep Liquor as a Unique Source of Nutrients." Fermentation 9, no. 4 (April 3, 2023): 351. http://dx.doi.org/10.3390/fermentation9040351.
Full textAbstract:
The relevance of this work lies in the fact that it is the first time that corn steep liquor (CSL) has been proposed as a unique source of nutrients for producing biosurfactants in a controlled fermentation and Aneurinibacillus aneurinilyticus, isolated from CSL, has been evaluated for producing extracellular and cell-bound biosurfactants in a controlled fermentation, using secondary raw materials as a source of nutrients. In the present study, A. aneurinilyticus was inoculated into the culture medium containing sterilized CSL solutions (100–400 g L−1) and incubated using different temperatures (20–60 °C) and fermentation times (8–30 days). The dependent variables under study were the concentration of extracellular biosurfactants and cell-bound biosurfactant production in terms of critical micellar concentration (CMC), as well as the C/N ratio for cell-bound biosurfactant extracts. It was observed that CSL could increase the concentration of extracellular biosurfactants produced by A. aneurinilyticus if these were fermented during 19 days at 40 °C, using 250 g L−1 of CSL; a mean value of 6 g L−1 for extracellular biosurfactants and favorable CMC concentrations enabled the detection of cell-bound biosurfactant extracts under these conditions. Hence, these conditions could be considered optimal for producing both extracellular and cell-bound biosurfactants from CSL.
8
Shaileshbhai, Mr Gopani Juvin, and Ms Trupti Pandya. "Microbial Production Bioemulsifier and its Applications." International Journal for Research in Applied Science and Engineering Technology 11, no. 7 (July 31, 2023): 637–47. http://dx.doi.org/10.22214/ijraset.2023.54727.
Full textAbstract:
Abstract: According to various research, Biosurfactants are generally composed of amphipathic motes that have both hydrophilic and hydrophobic ingredients. The hydrophilic composites generally correspond to positive, negative, or amphoteric charged ions, whereas the hydrophobic composites are made up of a long chain of fatty acids. It is getting important in biotechnology products for numerous industrial applications including in food, cosmetics and cleaning products, medicine, drug, and oil and gas. Bacterial cells produce an admixture of biosurfactant( BS) lipids with the help of which oil is dispersed into veritably fine droplets and therefore the bioavailability of CO is increased. Biosurfactants are surface-active site compounds produced by microorganisms. Biosurfactant generally refers to surfactants of microbial origin. Most of the biosurfactants produced by microbes are synthesized extracellularly and numerous microbes are known to produce biosurfactants in large relative amounts. This study concentrates on the insulation of biosurfactant-producing microorganisms from soil samples.
9
Cai, Qinhong, Baiyu Zhang, Bing Chen, Tong Cao, and Ze Lv. "Biosurfactant produced by a Rhodococcus erythropolis mutant as an oil spill response agent." Water Quality Research Journal 51, no. 2 (January 20, 2016): 97–105. http://dx.doi.org/10.2166/wqrjc.2016.025.
Full textAbstract:
Biosurfactants have been considered as superior alternatives to currently used surfactants as they are generally more biodegradable, less toxic, and better at enhancing biodegradation. However, the application of biosurfactants is limited by the availability of economic biosurfactants and the corresponding producers that can work effectively. Hyperproducers generated by metabolic engineering of biosurfactant producers are highly desired to overcome this obstacle. A Rhodococcus erythropolis SB-1A strain was isolated from offshore oily water samples. One of its mutants derived by random mutagenesis with ultraviolet radiation, producing high levels of biosurfactants, was selected by the oil spreading technique. The mutant produces biosurfactants with critical micelle dilutions approximately four times those of the parent strain. The results obtained with thin layer chromatography indicated the produced biosurfactant remained unchanged between the mutant and the parent strain. In addition, the produced biosurfactants were recovered with solvent extraction and applied as the oil spill response agents. Based on the baffled flask test (BFT) results, the dispersion efficiency of the biosurfactants produced by the mutant is higher than that induced by the parent strain. When compared with Corexit dispersants, it was found that the produced biosurfactants performed better than Corexit 9527 and were comparable with Corexit 9500.
10
Zamani Beidokhti, Majid, and Daryoush Yousefi Kebria. "Evaluation of biosurfactant production by Sporosarcina halophila and its application in crude oil remediation." Environmental Health Engineering and Management 9, no. 4 (November 5, 2022): 375–79. http://dx.doi.org/10.34172/ehem.2022.40.
Full textAbstract:
Background: Biosurfactants are valuable microbial products that have significant applications in various industries. The advantages of these compounds are biodegradability, low toxicity, activity in difficult environmental conditions, and the ability to produce oil residues and compounds from the surface of seawater and soils contaminated with oil compounds. The aim of this study was to evaluate the ability of biosurfactants production by Sporosarcina halophila. Methods: For this purpose, to detect the production of biosurfactant by Sporosarcina halophila, quantitative and qualitative screening methods such as hemolysis, oil spreading test and emulsification index test were used. Finally, different concentrations of crude oil in the bacterial growth medium were used to see that this strain can decompose crude oil using biosurfactant production to continue its growth or not. Results: The results showed that this strain is able to produce biosurfactants in oil hemolysis and spreading test with emulsifying activity of more than 30%, indicating that this strain is a suitable strain for biosurfactant production. Also, this strain could grow in the presence of crude oil in its medium as only carbon resource by biosurfactant production. Conclusion: This study showed that the metabolites derived from Sporosarcina halophila strain have emulsifying properties and can be considered as a potent strain in the production of biosurfactants. Also, it was concluded that these biosurfactants are applicable for many different industrial or environmental fields such as bioremediation of crude oil from soil or water by Sporosarcina halophila strain.
11
Ortansa, Csutak, Sarbu Ionela, Rusu Elena, Tatiana Vassu, and Viorica Corbu. "Antimicrobial and Antiadhesion Activity of Biosurfactants from Rhodotorula glutinis Grown on n-dodecane." Revista de Chimie 71, no. 5 (May 29, 2020): 99–105. http://dx.doi.org/10.37358/rc.20.5.8117.
Full textAbstract:
The biosurfactants represent a class of biocompounds of growing interest for biomedical applications. The yeast strain Rhodotorula glutinis CMGB-RG1 assimilated normal dodecane (C12H26) with high rates, producing biosurfactants with constant emulsification index (29%) after two, respectively, three days. Best antimicrobial activity was recorded for the [50X] concentrated biosurfactant against Candida krusei CMGB-Y8 from vaginal infections. The crude biosurfactant showed high microbial inhibition of biofilms formed by Candida krusei CMGB 94 (35%), while for C. krusei CMGB-Y8 best results were obtained with [50X] concentrated biosurfactant (47%) due to the presence of more effective mechanism for biofilm formation and adhesion. The results represent a basis for further studies regarding the characterization of antimicrobial mechanism of action of R. glutinis biosurfactants against a large range of human pathogenic microorganisms.
12
Osmorskaya, Zlata, Nikita Ezhkin, Alexander Gordeev, Liliya Biktasheva, and Svetlana Selivanovskaya. "Characterization of biosurfactants produced by the Bacillus amyloliquefaciens ВКМА В-12464." E3S Web of Conferences 392 (2023): 01013. http://dx.doi.org/10.1051/e3sconf/202339201013.
Full textAbstract:
Biosurfactants are amphiphilic substances with a wide range of uses. Biosurfactants are being researched in various areas of biotechnology. They are especially widely used in the development of new environmentally friendly methods for increasing oil production, as well as in agriculture as an effective biocontrol agent. However, the possibility of finding strains producing biosurfactants and the efficiency of their production is a separate task for research. The aim of this work was to evaluate the dynamics of biosurfactant production by the Bacillus amyloliquefaciens VKMA B-12464 strain, as well as to evaluate its chemical nature. During the research, the resulting biosurfactant was characterized by TLC and IR spectroscopy. It has been established that the biosurfactant produced by Bacillus amyloliquefaciens belongs to lipopeptides, and its highest yield is observed on the 7th day of cultivation.
13
Martínez-Arcos, Andrea, Mònica Reig, José Manuel Cruz, José Luis Cortina, Ana Belén Moldes, and Xanel Vecino. "Evaluation of Calcium Alginate-Based Biopolymers as Potential Component of Membranes for Recovering Biosurfactants from Corn Steep Water." Water 13, no. 17 (August 31, 2021): 2396. http://dx.doi.org/10.3390/w13172396.
Full textAbstract:
Corn steep water (CSW) is a complex agro-food stream that is used as a source of cost-competitive biosurfactants, since they are produced spontaneously in the steeping process of corn, avoiding production costs. Nevertheless, the extraction of biosurfactants from CSW using sustainable processes is still a challenge. Consequently, the use of calcium alginate membranes could present a novel and sustainable technology for recovering biosurfactants from aqueous streams. Therefore, the aim of this work is to evaluate calcium alginate-based biopolymers, without and with the presence of grape marc as an additive, as a key component of membranes for the recovery of biosurfactants in corn steep water. Biosurfactants are present in CSW, together with other inorganic solutes and biomolecules, such as organic acids, sugars, cations, anions as well as metals. Hence, the competition of these mentioned compounds for the active sites of the calcium alginate-based biopolymers was high. However, they showed a good adsorption capacity for biosurfactants, recovering around 55 ± 2% and 47 ± 1%, of biosurfactants from CSW using both calcium alginate-based biopolymers, with and without biodegraded grape marc. Regarding adsorption capacity, it was 54.8 ± 0.6 mg biosurfactant/g bioadsorbent for the biopolymer containing grape marc, and 46.8 ± 0.4 mg biosurfactant/g bioadsorbent for the calcium alginate-based biopolymer alone. Based on these results, it could be postulated that the formulation of green membranes, based on calcium alginate-based polymers, could be an interesting alternative for the recovery of biosurfactants from aqueous streams including CSW.
14
Youssef, Noha H., Kathleen E. Duncan, and Michael J. McInerney. "Importance of 3-Hydroxy Fatty Acid Composition of Lipopeptides for Biosurfactant Activity." Applied and Environmental Microbiology 71, no. 12 (December 2005): 7690–95. http://dx.doi.org/10.1128/aem.71.12.7690-7695.2005.
Full textAbstract:
ABSTRACT Biosurfactant production may be an economic approach to improving oil recovery. To obtain candidates most suitable for oil recovery, 207 strains, mostly belonging to the genus Bacillus, were tested for growth and biosurfactant production in medium with 5% NaCl under aerobic and anaerobic conditions. All strains grew aerobically with 5% NaCl, and 147 strains produced a biosurfactant. Thirty-five strains grew anaerobically with 5% NaCl, and two produced a biosurfactant. In order to relate structural differences to activity, eight lipopeptide biosurfactants with different specific activities produced by various Bacillus species were purified by a new protocol. The amino acid compositions of the eight lipopeptides were the same (Glu/Gln:Asp/Asn:Val:Leu, 1:1:1:4), but the fatty acid compositions differed. Multiple regression analysis showed that the specific biosurfactant activity depended on the ratios of both iso to normal even-numbered fatty acids and anteiso to iso odd-numbered fatty acids. A multiple regression model accurately predicted the specific biosurfactant activities of four newly purified biosurfactants (r 2 = 0.91). The fatty acid composition of the biosurfactant produced by Bacillus subtilis subsp. subtilis strain T89-42 was altered by the addition of branched-chain amino acids to the growth medium. The specific activities of biosurfactants produced in cultures with different amino acid additions were accurately predicted by the multiple regression model derived from the fatty acid compositions (r 2 = 0.95). Our work shows that many strains of Bacillus mojavensis and Bacillus subtilis produce biosurfactants and that the fatty acid composition is important for biosurfactant activity.
15
Tadayon Tajabadi, Mohammadhassan, Asyeih Sabernejad, and Mohsen Khalili Najafabadi. "Biosurfactant-producing Microorganisms: Potential for Bioremediation of Organic and Inorganic Pollutants." Research in Biotechnology and Environmental Science 2, no. 2 (June 16, 2023): 18–23. http://dx.doi.org/10.58803/rbes.v2i2.13.
Full textAbstract:
The contamination of soil and water by heavy metals and hydrophobic organic compounds poses a significant threat to the environment. Traditional physicochemical methods for remediation are often expensive and environmentally unfriendly, while bioremediation offers a more eco-compatible and economically feasible alternative. Bioremediation utilizes microorganisms, plants, or microbial/plant enzymes to detoxify contaminants in various environments. Biosurfactants, amphiphilic compounds produced by microorganisms, play a crucial role in enhancing bioremediation effectiveness. They increase substrate surface area, create microenvironments, and promote emulsification, thereby facilitating the removal of pollutants. This article provided a comprehensive overview of biosurfactant-producing microorganisms and their potential in the bioremediation of organic and inorganic pollutants. The types and classifications of biosurfactants as well as the factors influencing their production were discussed. Various microorganisms, including bacteria, fungi, and yeasts, were identified as biosurfactant producers. This study outlined the production process and highlighted the importance of optimizing growth conditions for high-quality biosurfactant production. The applications of biosurfactants in remediation were explored by emphasizing their ability to enhance biodegradation, remove heavy metals, and increase hydrocarbon bioavailability. Several studies demonstrating the efficacy of biosurfactant-producing microorganisms in bioremediation were presented. The potential limitations and challenges associated with biosurfactant application in situ were also discussed. In conclusion, the controlled use of biosurfactants could offer promising prospects for the efficient and sustainable cleanup of contaminated sites, contributing to environmental remediation efforts.
16
Sena, Hellen Holanda, Michele Alves Sanches, Diego Fernando Silva Rocha, Walter Oliva Pinto Filho Segundo, Érica Simplício de Souza, and João Vicente Braga de Souza. "Production of Biosurfactants by Soil Fungi Isolated from the Amazon Forest." International Journal of Microbiology 2018 (2018): 1–8. http://dx.doi.org/10.1155/2018/5684261.
Full textAbstract:
Biosurfactants are surface-active compounds that have sparked interest in recent years because of their environmental advantages over conventional surfactants. The aim of this study was to investigate the production of biosurfactants by soil fungi isolated from the Amazon forest. Fungi colonies were isolated from soil samples and screened for biosurfactant production in submerged fermentation. In addition, the influences of bioprocess factors (carbon source, nitrogen source, pH, and fermentation time) were investigated. Finally, the biosurfactant produced was semipurified and submitted to stability tests. One hundred fungal cultures were obtained from the soil samples, identified by micromorphology, and submitted to screening for biosurfactant production. Sixty-one strains produced biosurfactants. The strainPenicillium8CC2 showed the highest emulsification index (54.2%). The optimized bioprocess conditions for biosurfactant production byPenicillium8CC2 were as follows: soybean oil, 20 g/L; yeast extract, 30 g/L; pH 9; duration of 9 days. The semipurified biosurfactant showed stability after heating at 100°C for 60 min and after the addition of 30% NaCl (w/v). Tween 80 (0.2% w/v), a conventional surfactant, was used as the control.
17
Vaijayanti, Mahulkar Ankita Vidyadhar. "Comparative study of antimicrobial efficiency of biosurfactant producing Pseudomonas spp. from different soil samples." Journal of Applied and Advanced Research 5 (September 6, 2020): 1. http://dx.doi.org/10.21839/jaar.2020.v5.318.
Full textAbstract:
Amphiphilic biosurfactants are surface-active biological molecules secreted by hydrocarbanoclastic microorganisms. Biosurfactants are eco-friendly, less toxic, biodegradable, and low-cost material, so it has more advantages over chemical surfactants. In this research, Pseudomonas spp., biosurfactant producing microorganisms isolated from different sources of soil samples. IS1, IS2, IS3, IS4 isolates obtained from Garden soil sample; Metal contaminated soil sample; Petroleum contaminated soil sample; Oil contaminated soil sample; respectively. Each isolates identified as Pseudomonas spp. Furthermore, screened for biosurfactant producers. Each isolate showed positive results for the hemolysis test, drop collapse test, oil displacement test, and emulsification test. All isolate incubated in mineral salt medium for biosurfactant production. Biosurfactant extracted from IS1, IS2, IS3, IS4 showed 35%, 65%, 20%, 52% emulsification index respectively. Antimicrobial activity of extracted biosurfactants against pathogenic microorganisms checked by agar cup method. IS2 isolate shows the highest antimicrobial activity among all. All isolate showed a higher zone of inhibition against gram-positive microorganisms than gram-negative microbes. The purpose of this study involves the assessment of the antimicrobial activity of biosurfactant producers from the soil environment.
18
Sharma, Pushpinder, and Nivedita Sharma. "Microbial Biosurfactants-an Ecofriendly Boon to Industries for Green Revolution." Recent Patents on Biotechnology 14, no. 3 (September 25, 2020): 169–83. http://dx.doi.org/10.2174/1872208313666191212094628.
Full textAbstract:
Biosurfactants have a biological origin, and are widely known as surface active agents. Different classes of biosurfactant have significant importance in both the biotechnological and microbiological arena. Pseudomonas aeruginosa, Bacillus subtilis and Candida sp. are important classes of microorganisms that are highly investigated for the production of rhamnolipids (RLs) biosurfactants. Rhamnolipids have unique surface activity and have gained interest in various industrial applications. Due to their high biodegradability, renewability and functionally maintenance at extreme conditions, microbial biosurfactants are more advantageous than chemical-based biosurfactants. Biosurfactants produced by microorganisms are a potential candidate for biodegradation, environmental cleanup of pollutants and also play a role in the heavy metal removal of metallurgical industries also many patents have been filed. Therefore, greater attention has been paid to biosurfactants and identifying their potential applications for further studies.
19
SAIMMAI, Atipan, Wiboon RIANSA-NGAWONG, Suppasil MANEERAT, and Paweena DIKIT. "Application of Biosurfactants in the Medical Field." Walailak Journal of Science and Technology (WJST) 17, no. 2 (June 20, 2019): 154–66. http://dx.doi.org/10.48048/wjst.2020.4748.
Full textAbstract:
It is generally known that both chemical substances and many kinds of microorganism can be used to produce surfactants or surface-active compounds. Surfactants derived from microorganisms are called biosurfactants, or bio-surface active compounds. Recently, biosurfactants have become more interesting because of their advantages, such as less toxicity and more degradability, which cannot be found in traditional surfactants. Biosurfactant production faces some problems, such as a high cost of production. In the medical field, biosurfactants are attractive, because the products from biosurfactants can be used effectively in small amounts. This can compensate for the high cost of production. In addition, there have been many great discoveries of biosurfactants in the medical field.
20
Wardhani, Riuh, Dirayah Rauf Husain, and Fuad Gani. "Reduction of Surface Tension of Petroleum Using Hydrocarbon Degrading Bacterial Activity." ASM Science Journal 17 (May 27, 2022): 1–6. http://dx.doi.org/10.32802/asmscj.2022.1273.
Full textAbstract:
Hydrocarbon degrading bacteria produce biosurfactants, which facilitate the biodegradation process. This is the first step towards getting access to interactions between the bacteria's hydrophilic surface and the hydrophobic surface of the hydrocarbons. Due to their amphipathic nature, biosurfactants facilitate this interaction. The focus of the study is to obtain hydrocarbon-degrading bacteria isolates and evaluate biosurfactant activity in reducing water surface tension. Hydrocarbon-degrading bacteria were isolated from contaminated marine sediment samples and consequently cultured on artificial seawater media with the addition of petroleum hydrocarbons as a carbon source. Surface tension reduction of biosurfactants was measured using a digital K20-EasyDyne tensiometer (KRÜSS: Hamburg, Germany). The study indicates that the isolates have biodegradation activity and reduced water surface tension by 22.14 mN/m, the data demonstrated that biosurfactant production was most effective on the third day of the exponential phase incubation. These studies demonstrate the effectiveness of hydrocarbon-degrading bacteria to produce biosurfactants as biodegradation agents to solve the problem of oil pollution.
21
Tabrizi Rayeni, Laya, and Shahla Soltani Nezhad. "Characterization of Biosurfactant Produced by Probiotic Bacteria Isolated from Human Breast Milk." International Journal of Basic Science in Medicine 3, no. 1 (February 14, 2018): 18–24. http://dx.doi.org/10.15171/ijbsm.2018.04.
Full textAbstract:
Background: Breast milk is an important nutrient source for rapidly growing neonates since breastfeeding protects the newborn against some disease. This effect may be due to the useful and natural microflora of breast milk. Biosurfactants are unique amphipathic compounds produced by some microorganisms. The present study demonstrates the isolation and characterization of biosurfactant producing bacteria from human breast milk samples. Methods: The human breast milk samples were collected aseptically and then cultured in MRS agar media. The biosurfactant producing ability of the isolated strains was investigated by hemolytic assay, oil spreading method, drop collapse test and emulsification index assay. The screened isolates were identified by 16S rDNA gene sequencing analysis. In vitro antibacterial activities of biosurfactants against some Gram-positive and Gram negative bacteria were investigated by the agar disc diffusion method. This biosurfactant was characterized with Fourier transform infrared spectroscopy (FTIR). Results: In this study, 337 different colonies were isolated from 42 breast milk samples. The best isolates were identified as Pediococcus pentosaceus HM-1, Pediococcus pentosaceus HM-2 and Pediococcus pentosaceus HM-3 based on microscopic and 16S rDNA gene sequencing analysis. The biosurfactant extracted from screened strains exhibited a broad spectrum of antagonistic activity against some pathogenic bacteria. The results showed similarity to lipopeptide biosurfactants like surfactin. Conclusion: Bacterial strains isolated in this study could be valuable sources for novel biosurfactants. The Human breast milk could be a safe source for isolation of biosurfactant producing probiotic bacteria and for improve intestinal microflora of infants.
22
Mallik, Tapati, and Debashis Banerjee. "BIOSURFACTANTS: THE POTENTIAL GREEN SURFACTANTS IN THE 21ST CENTURY." Journal of Advanced Scientific Research 13, no. 01 (February 10, 2022): 97–106. http://dx.doi.org/10.55218/jasr.202213110.
Full textAbstract:
Biosurfactants are amphiphilic microbial molecules with hydrophilic and hydrophobic moieties that partition at liquid/liquid, liquid/gas or liquid/solid interfaces. These unique characteristics allow these biomolecules to play a key role in emulsification, foam formation, detergency and dispersal, which makes them ideal for use in different industrial sectors. The production of biosurfactants is considered to be the prime development in industrial technology in the 21st century. Coupled with this feature, increasing public awareness of environmental pollution promotes the search and development of emerging technologies that help in cleaning up of organic and inorganic contaminants. Biosurfactants and biosurfactant producing microorganisms provides an alternative and eco-friendly method of remediation technology of environment contamination with these pollutants. Thus, biosurfactant production, possibility of their application as renewable resources and “green” products has now been the subject of extensive research in recent years. This review deals with the accumulated knowledge regarding biosurfactants gained over the years and possibility of their applications in various industrial fields, in oil recovery, in keeping the environments green, in medicinal sectors. The economic issue regarding the production of biosurfactants on an industrial basis in this regard also has been critically dealt with.
23
Biktasheva, Liliya, Alexander Gordeev, Anastasia Kirichenko, Polina Kuryntseva, and Svetlana Selivanovskaya. "Screening of Microorganisms from Wastes and Identification of the Optimal Substrate for Biosurfactant Production." Microbiology Research 15, no. 1 (January 6, 2024): 152–63. http://dx.doi.org/10.3390/microbiolres15010010.
Full textAbstract:
The production of biosurfactants from organic wastes has received significant attention due to its potential cost savings. This study involved the isolation of biosurfactant-producing microorganisms from waste sources. The surfactant properties of the 37 studied isolates were assessed by reducing surface tension and their emulsifying properties, determined by the emulsification index E24. We assessed the ability of these isolated strains to produce biosurfactants using various waste substrates, namely potato peelings, waste cooking oil and sunflower cake. Our results showed that sunflower cake exhibited better growth and biosurfactant production for most of the strains studied. This highlights that sunflower cake is a potentially effective and economical substrate for the production of biosurfactants. The most effective strains allowing to achieve an emulsification index above 50% and reduce surface tension below 40 mN m−1 were Enterobacter sp. 2pp, strain 2wfo, Peribacillus sp. 1mo, Sphingomonas sp. 2mo, Ochrobactrum sp. 5mo, Shouchella sp. 6mo, Bacillus sp. 1os, Bacillus sp. 2os. Among these strains, both previously known strains as biosurfactant producers and previously unknown strains were found. Thus, we found that among representatives of the genus Sphingomonas there are effective producers of biosurfactants. The highest yield of biosurfactant on a medium with glycerol and glucose was shown by the Bacillus sp. 2os strain of 0.501 and 0.636 g L−1, respectively.
24
Sulistyarso, Harry Budiharjo, Joko Pamungkas, Sri Rahayu Gusmarwani, Indah Widyaningsih, and Kevin Yudha Perwira. "Field Test of U-Champ Biosurfactant in Oil Spill Bioremediation." International Journal of Recent Technology and Engineering (IJRTE) 10, no. 3 (September 30, 2021): 75–78. http://dx.doi.org/10.35940/ijrte.c6428.0910321.
Full textAbstract:
One of the serious environmental problems of the oil and gas industry is handling oil spills. Oil spills can be managed by using bioremediation in the form of biosurfactants. In this research, biosurfactants called U-Champ were used which can impact in remediation of oil spills. The research conducted was testing the effectiveness of biosurfactants on Cepu Field contaminated soil in reducing TPH and heavy metals. The method is done by stirring the contaminated soil with various concentrations of biosurfactant product in an artificial tank. Based on the test results, a decline in TPH and TCLP along with the increase in U-Champ biosurfactant concentration was used. The addition of 6 Liters of biosurfactant gave the most significant reduction. Some of the heavy metals in the soil decrease, but some also have increased. As an example, it can be seen in Ba metal, the addition of U-Champ biosurfactant as much as 1.5 L (0.3% v / v) resulted in a decrease in TCLP of 11.085%. The qualitative result is evidenced by the growth of various plants in each artificial tank.
25
Lepo, Joe Eugene, M. Patricia Hancock, Candy L. Zuleger, Katharine Roupp-Edwards, Tim Reilly, and David E. Fritz. "EFFECTIVENESS AND SAFETY OF BIOSURFACTANTS AS AGENTS OF OIL SPILL RESPONSE." International Oil Spill Conference Proceedings 1997, no. 1 (April 1, 1997): 687–96. http://dx.doi.org/10.7901/2169-3358-1997-1-687.
Full textAbstract:
ABSTRACT Biosurfactants play an important role in facilitating the natural biodegradation of petroleum hydrocarbons. In order to characterize the biosurfactant-producing potential of environments at risk of anthropogenic oil contamination, we collected a number of distinct marine bacteria that emulsified crude oil from relatively pristine and from polluted areas. The bacteria belonged to diverse taxonomic groups and varied widely in oil-emulsifying capability, surface tension reduction of culture media, and hydrophobicity. We used laboratory assays that directly evaluated biosurfactant preparations from these and other strains for use as cleaning agents for oiled surfaces. The subjects of the study were the oil emulsification capabilities and the surface-active characteristics of the biosurfactants, which were compared to those of selected synthetic surfactants. We also examined the ability of different biosurfactants and synthetic surfactants to wash oil from porcelain tiles. To assess the efficacy of biosurfactants as enhancers of oil biodegradation, we applied preparations of biosurfactants along with our standard mix of hydrocarbon-degrading bacteria in either shake-flask experiments or in sandy-beach microcosms. In general, surfactants of either class (biosurfactants or synthetic surfactants) performed similarly in all of these experiments. Although some biosurfactants show merit as cleaning agents, the current production technology may not make their use cost-effective. Safety issues relating to biosurfactants were addressed by examining their toxicity and their biodegradability (and thus persistence in the environment) relative to selected synthetic surfactants. Standard toxicology bioassays were used: (1) the mysid, 7-day chronic estimator, and (2) the inland silverside 7-day chronic estimator. The biodegradability of biosurfactants and synthetic surfactants was compared by measuring the increased biochemical oxygen demand generated by the surfactants in raw seawater. Some biosurfactants were indeed highly biodegradable; however, we found no tight association of biodegradability to either class of surfactants. These results suggest that the environmental safety of surfactants is best evaluated on the basis of toxicity and degradability data for specific cases.
26
Javadi, Ali, Mohamad Reza Pourmand, Javad Hamedi, Fatemeh Gharebaghi, Zohre Baseri, Razieh Mohammadzadeh, and Seyyed Saeed Eshraghi. "Evaluation of anti-biofilm potential of biosurfactant extracted from Nocardia species." Folia Medica 63, no. 3 (June 30, 2021): 392–99. http://dx.doi.org/10.3897/folmed.63.e54386.
Full textAbstract:
Introduction: Bacterial natural products such as biosurfactants and surface-active agents are important compounds which exhibit many applications in the fields of medicine. Aim: The aim of the present study was to isolate and identify Nocardia strains with high biosurfactant production and antibiofilm ability. Materials and methods: In the present study, a biosurfactant producing Nocardia species was isolated and identified by a laboratory method. Nocardia species were initially screened and then tested for their ability to produce biosurfactant. The oil spreading test and the surface tension measurements showed that one strain was a biosurfactant producer. The strain with the best surface activity results was selected for further studies and identified by 16S rRNA gene sequencing method. Fourier transform infrared spectroscopy (FTIR) and compositional analysis proved a biosurfactant structure. Results: Oil spreading test and blue agar plate test confirmed biosurfactants and extracellular anionic glycolipids. E24% assay using olive oil revealed strong emulsifying characteristic of the extracted biosurfactant with 100% emulsifying strength. FTIR spectrum indicated the presence of aliphatic hydrocarbon chain (lipid) along with the polysaccharide portion, confirming the glycolipid nature of the biosurfactant. The stability of the biosurfactant produced in different conditions was significant. Increasing concentration of BS significantly inhibited Pseudomonas aeruginosa biofilm. Conclusions: N. coubleae can be a representative of the genus Nocardia for the production of biosurfactants with beneficial physicochemical properties.
27
Al-Tahhan, Ragheb A., Todd R. Sandrin, Adria A. Bodour, and Raina M. Maier. "Rhamnolipid-Induced Removal of Lipopolysaccharide from Pseudomonas aeruginosa: Effect on Cell Surface Properties and Interaction with Hydrophobic Substrates." Applied and Environmental Microbiology 66, no. 8 (August 1, 2000): 3262–68. http://dx.doi.org/10.1128/aem.66.8.3262-3268.2000.
Full textAbstract:
ABSTRACT Little is known about the interaction of biosurfactants with bacterial cells. Recent work in the area of biodegradation suggests that there are two mechanisms by which biosurfactants enhance the biodegradation of slightly soluble organic compounds. First, biosurfactants can solubilize hydrophobic compounds within micelle structures, effectively increasing the apparent aqueous solubility of the organic compound and its availability for uptake by a cell. Second, biosurfactants can cause the cell surface to become more hydrophobic, thereby increasing the association of the cell with the slightly soluble substrate. Since the second mechanism requires very low levels of added biosurfactant, it is the more intriguing of the two mechanisms from the perspective of enhancing the biodegradation process. This is because, in practical terms, addition of low levels of biosurfactants will be more cost-effective for bioremediation. To successfully optimize the use of biosurfactants in the bioremediation process, their effect on cell surfaces must be understood. We report here that rhamnolipid biosurfactant causes the cell surface ofPseudomonas spp. to become hydrophobic through release of lipopolysaccharide (LPS). In this study, two Pseudomonas aeruginosa strains were grown on glucose and hexadecane to investigate the chemical and structural changes that occur in the presence of a rhamnolipid biosurfactant. Results showed that rhamnolipids caused an overall loss in cellular fatty acid content. Loss of fatty acids was due to release of LPS from the outer membrane, as demonstrated by 2-keto-3-deoxyoctonic acid and sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis and further confirmed by scanning electron microscopy. The amount of LPS loss was found to be dependent on rhamnolipid concentration, but significant loss occurred even at concentrations less than the critical micelle concentration. We conclude that rhamnolipid-induced LPS release is the probable mechanism of enhanced cell surface hydrophobicity.
28
Fazli, Rahmad Rizki, Latifah Hanum, Mieke Alvionita, and Said Ali Akbar. "VARIASI SUMBER KARBON TERHADAP PRODUKSI BIOSURFAKTAN OLEH BAKTERI HALOFILIK ISOLAT TAMBAK GARAM KAJHU ACEH BESAR." Lantanida Journal 10, no. 1 (July 30, 2022): 75. http://dx.doi.org/10.22373/lj.v10i1.14108.
Full textAbstract:
This research has isolated one type of halophilic bacterial strain from the salt pond of Kajhu Village with the code KJ-AB2. This bacterium grew optimally at 10% (w/v) NaCl so it was classified as moderate halophilic bacteria. Several types of halophilic bacteria are known to produce biosurfactants. Biosurfactants are surface active agents that are widely used in several pharmaceutical, food dan petroleum industries. The production of biosurfactants is strongly influenced by the type of carbon source used for bacterial growth. Olive oil, palm oil, sunflower oil, glucose dan glycerol are types of carbon sources used to see their effect in producing biosurfactants. This isolate growed well on the five carbon sources with an average OD600 2.001±0.293 at 72 hours of fermentation. Biosurfactant production was measured by oil spreading test (OST) every 24 hours until 120 hours of fermentation. The highest OST value was obtained when KJ-AB2 isolate was grown on biosurfactant production media using olive oil, sunflower oil dan palm oil with an average OST value of 5.6±0.6 cm, 4.7±0.6 cm, dan 4.6±0.7 cm, respectively.
29
Sugihartuti, Rahmi, Munawer Pradana, Lilik Maslachah, Sri Chusniati, Wiwiek Tyaningsih, Dadik Rahardjo, and Soeharsono Soeharsono. "Isolation and Identification of Bacterial Biosurfactant Activity from Mangrove Sediments." Journal of Basic Medical Veterinary 13, no. 1 (June 5, 2024): 38–48. http://dx.doi.org/10.20473/jbmv.v13i1.58266.
Full textAbstract:
Background: Ujung Pangkah mangroves are reported to have been contaminated with heavy metals. Such heavy metals can induce microorganisms to produce biosurfactants. Biosurfactants with amphiphilic characteristics can lower surface tension. Biosurfactants can be used as antibacterial, antifungal, and antiviral for biomedical purposes. Purpose: This study aimed to identify and test the activity of biosurfactant isolates of bacteria from Ujung Pangkah Mangrove sediments, Gresik Regency. Methods: Biosurfactant activity test methods include emulsification index, oil spreading, drop collapse, and parafilm test. Results: The results of the identification of bacterial isolates in this study obtained the genus Bacillus sp. because bacterial isolates show rod shape, Gram-positive, aerobic, and have ellipse-shaped endospores on the subterminal. The results of the biosurfactant activity test with the Emulsification index method showed an average result of 54.39% and the results of the biosurfactant activity test with the oil spreading method showed that there was a clear zone. The average result of the clear zone obtained is 54.83 mm. The average result in the parafilm test was 8.02 mm and the drop collapse test showed positive results characterized by falling and spreading of bacterial isolate fermentation broth supernatants.
30
Verma, Rupa, Mukul Agrawal, Abhay Dundung, and Ladly Rani. "Screening of Biosurfactant Production in Bacteria Isolated from Oil and Pesticide Contaminated Soil of Ranchi District." Journal of Scientific Research 66, no. 04 (2022): 29–34. http://dx.doi.org/10.37398/jsr.2022.660405.
Full textAbstract:
Biosurfactants, the surface-active compounds are produced by a few microorganisms. As such biosurfactants are superior to synthetic surfactants in terms of cost of production and industrial application. Present study deals with the isolation, characterization, screening, and extraction of biosurfactant producing bacteria from motor oil dumped and pesticide contaminated agricultural fields of Ranchi District, Jharkhand, India. Mineral salt medium (MSM) supplemented with hydrocarbon was used for the enrichment of putative biosurfactant producers. Out of ten isolates, five isolates (F1 to F5) showed growth on hydrocarbon supplemented plate suggesting its use as main carbon source. Three isolates namely F1, F4 and F5 were from the waste oil contaminated soil and remaining two (F2 and F3) from pesticide contaminated site. Based on traditional microbiological methods, characterization and identification were made which showed that F1, F4 and F5 isolates belong to Staphylococcus aureus and F2 and F3 to Bacillus subtilis species. Biosurfactant production was tested by hemolysis, emulsification index (E24) and drop collapsing tests, results showed positive test for all the assay suggesting the potential of biosurfactant production by all the five isolates. Among all the five isolates, F1 showed maximum emulsification index (44.44%) followed by F4 (35%) both belonging to strains of Staphylococcus aureus. The remaining isolates F2, F3 and F5 also showed appreciable level of E24 (24-28%). Biosurfactants produced by all the five isolates were extracted using solvents, the dry weight showed close correlation with E24. Further work is needed to confirm the identity of all the isolates using 16S rRNA sequencing and chemical characteristics of biosurfactants employing standard analytical techniques.
31
Rawal, Nikhil, and Rukhsar Ansari. "Understanding Pathways, Substrates, and Media Optimization to Maximize Microbial Biosurfactant Production." International Journal for Research in Applied Science and Engineering Technology 12, no. 4 (April 30, 2024): 1342–51. http://dx.doi.org/10.22214/ijraset.2024.60043.
Full textAbstract:
Abstract: The production of amphiphilic chemicals, known as biosurfactants, by different microorganisms is essential for lowering interfacial and surface tension. The pathways of biosurfactant synthesis are examined in this review, with a focus on the influence of carbon substrates on biosynthesis. It also covers the variety of substrates used in the production of biosurfactants, such as lignocellulosic wastes, agro-industrial wastes, oil processing byproducts, starch-rich wastes, industrial wastes derived from plants and animals, fish and chicken wastes, fruit and vegetable wastes, and wastes from fish and poultry operations. In addition, the optimization of media for the formation of biosurfactants is discussed, with particular attention to temperature, pH, concentration of salt, and sources of carbon and nitrogen. It is crucial to comprehend these routes and optimization techniques in order to address the economic and environmental issues related to waste management and increase the efficiency of biosurfactant production.
32
Lamilla, Claudio, Heidi Schalchli, Gabriela Briceño, Bárbara Leiva, Pamela Donoso-Piñol, Leticia Barrientos, Vanessa A. L. Rocha, Denise M. G. Freire, and M. Cristina Diez. "A Pesticide Biopurification System: A Source of Biosurfactant-Producing Bacteria with Environmental Biotechnology Applications." Agronomy 11, no. 4 (March 25, 2021): 624. http://dx.doi.org/10.3390/agronomy11040624.
Full textAbstract:
Biosurfactants, a wide group of compounds produced by different microorganisms, generally have less toxicity and are more biodegradable than synthetic surfactants. Biosurfactant-producing bacteria can be found in contaminated environments, such as soils receiving pesticide applications constantly, or in pesticides treatment systems where microorganisms are adapted to biodegrading pesticides. Five pesticide-tolerant bacteria previously isolated from a pesticide biopurification system were evaluated as biosurfactant-producers. Pseudomonas rhodesiae C4, Rhodococcus jialingiae C8 and Pseudomonas marginalis C9 strains were positive in qualitative tests. Biosurfactant production by these strains using Bushnell-Haas medium with olive oil at 2% (w/v) was evaluated as emulsification index, oil displacement, droplet collapse test and surface tension. After 144 h, these strains showed a similar emulsification index of >55%. The two Pseudomonas (C4 and C9) strains showed lower superficial tension compared with Rhodococcus strain (C8)—34.47, 37.44 and 47.55 mN/m for strains C4, C9 and C8, respectively. The chemical characterization of the biosurfactants revealed the presence of glycolipids in P. rhodesiae (C4) and glycopeptides in P. marginalis (C9). The degradation of chlorpyrifos increased from 39.2% to 51.6% when biosurfactants produced by P.rhodesiae (C4) were added (10%) with respect to the control. Therefore, biopurification systems are a relevant source of biosurfactant-producing bacteria with environmental biotechnology applications.
33
Jiang, Xinpeng, Xin Yan, Shanshan Gu, Yan Yang, Lili Zhao, Xinmiao He, Hongyan Chen, Junwei Ge, and Di Liu. "Biosurfactants of Lactobacillus helveticus for biodiversity inhibit the biofilm formation of Staphylococcus aureus and cell invasion." Future Microbiology 14, no. 13 (September 2019): 1133–46. http://dx.doi.org/10.2217/fmb-2018-0354.
Full textAbstract:
Aim: This study aimed to evaluate the differences of biosurfactants produced by two Lactobacillus helveticus strains against the biofilm formation of Staphylococcus aureus in vitro and in vivo. Materials & methods: Scanning electron microscopy, Real time-quantitative PCR (RT-qPCR) and cell assay were used to analyze the inhibiting effect of biosurfactants against biofilm formation. Results & conclusion: Results showed that the biosurfactants have anti-adhesive and inhibiting effects on biofilm formation in vivo and in vitro. The biofilm-formative genes and autoinducer-2 signaling regulated these characteristics, and the biosurfactant L. helveticus 27170 is better than that of 27058. Host cell adhesion and invasion results indicated that the biosurfactants L. helveticus prevented the S. aureus invading the host cell, which may be a new strategy to eliminate biofilms.
34
Al Hawaj, Maitham A., Arshad Farid, Mohammed Al Mohaini, Abdulkhaliq J. Alsalman, Muhammad Muzammal, Muhammad Hashim Khan, Arezoo Dadrasnia, et al. "Biosurfactant Screening and Antibiotic Analysis of Bacillus salmalaya." International Journal of Current Research and Review 14, no. 12 (2022): 56–64. http://dx.doi.org/10.31782/ijcrr.2022.141209.
Full textAbstract:
Introduction: Biosurfactants are made up of naturally occurring molecules such as lipopeptide, glycoprotein, lipoprotein, and fatty acids. Using biosurfactants rather than chemical and synthetic surfactants is safer. Objective: The aim of this study was to investigate biosurfactant properties and antibiotic resistance of soil bacteria, Bacillus salmalaya strain 139SI. Bacillus salmalaya produces biosurfactants, which capable of reducing surface tension of certain media, as they are surface-active molecules. Methods: In current study Parafilm M Test, Drop Collapse, Oil spreading methods for biosurfactants screening were used and antibiotic analysis was done using an aseptic technique. Results: 600μl biosurfactant (460 mg/ml) was the most effective volume to produce large diameter of oil spreading zone when 700 μl of oil was used. 0.23g freeze-dried supernatant powder mixed with 0.5 ml distilled water (460 mg/ml) produced larger clearing zone than powder mixed with 1 ml (230 mg/ml) and 1.5 ml distilled water (153 mg/ml). In cleaning activity of crude oil container, biosurfactant was able to clean oil in the container and this can be applied in cleaning oil waste from crude oil tank and can also be used to clean oil spillage. In antibiotic analysis study, ten soil bacteria isolates included Bacillus salmalaya were selected. Conclusion: All isolates had shown different sensitivity towards different antibiotics. Bacillus salmalaya showed sensitivity to almost all selected antibiotic compared to other isolates. This indicated that this strain is safe to be used as it was sensitive to many antibiotics.
35
Sharon, Abimbola Bowofoluwa, Eze Frank Ahuekwe, Elughi Gift Nzubechi, Olubukola Oziegbe, and Margaret Oniha. "Statistical optimization strategies on waste substrates for solving high-cost challenges in biosurfactants production: a review." IOP Conference Series: Earth and Environmental Science 1197, no. 1 (June 1, 2023): 012004. http://dx.doi.org/10.1088/1755-1315/1197/1/012004.
Full textAbstract:
Abstract Biosurfactants are bio-based amphiphilic molecules with extensive applications in various industries. These eco-friendly alternatives possess numerous advantages over chemical surfactants. However, high production costs hinder market competitiveness of biosurfactants. Production costs of synthetic surfactants range between $1-3/kg, while biosurfactants cost between $20-25/kg. Principal challenges hindering commercialization of biosurfactants are high costs of media constituents and downstream processing, accounting for 30% and 60-80% of production costs, respectively. Thus, cost-effective biosurfactant production would depend on the utilization of environment-friendly low-cost substrates and efficient product recovery. To this end, statistical tools such as Factorial Designs (FD) and Response Surface Methodology (RSM), are employed to optimize the production processes. FD as effective screening models comprise Plackett-Burman Design (PBD) and Taguchi design; and involves quantification of various significant factor effects including the main effect and level of dependency of one factor on the level of one or more factors. RSM predicts appropriate proportions of media constituents and optimal culture conditions; and is reportedly effective in reducing production cost and consequently, market price. Central Composite Design (CCD) and Box-Behnken Design (BBD) are common RSM for optimizing biosurfactants production. CCD assesses the relationship between one factor or more and a set of experimental variables. BBD is considered more proficient than CCD as it requires fewer experimental runs. Most recently, Artificial Neural Network which uses artificial intelligence-based tools to predict biosurfactant production using dependent variables of the process is gaining attention.
36
Parhar, Manreet, Kanwalpreet Kaur Bhullar, and Ridhima Arya. "Development of Efficacious and Less Toxic Endodontic Irrigants from Biosurfactants of Bacterial Origin – A Confocal Laser Scanning Microscope Study." Contemporary Clinical Dentistry 14, no. 4 (2023): 282–88. http://dx.doi.org/10.4103/ccd.ccd_377_23.
Full textAbstract:
Aim: The study compared the penetration depth and area of Bacillus subtilis and Pseudomonas aeruginosa biosurfactants inside the dentinal tubules against sodium hypochlorite at three levels (coronal, middle, and apical) under a confocal laser scanning microscope. Materials and Methodology: Three experimental groups and one control were created using freshly extracted thirty-five maxillary central incisors; Group 1: B. subtilis biosurfactant; Group 2: P. ae ruginosa biosurfactants; Group 3: sodium hypochlorite; and Group 4: distilled water. The experimental data were statistically analyzed by two-way analysis of variance, duly coupled with Tukey's post hoc test to draw concrete conclusions between paired comparisons, and P < 0.05 was considered statistically significant. Results: P. aeruginosa and B. subtilis biosurfactant showed the maximum mean penetration depth and area at all the level as compared to sodium hypochlorite. Conclusion: Biosurfactants showed better results than sodium hypochlorite in reaching to higher penetration depth and area at all the three levels.
37
Ezhkin, Nikita, Zlata Osmorskaya, Alexander Gordeev, Liliya Biktasheva, and Polina Kuryntseva. "Antifungal potential of biosurfactants produced by strains of Bacillus mojavensis PS17 against pathogens of the genera Fusarium and Verticillium." BIO Web of Conferences 71 (2023): 01102. http://dx.doi.org/10.1051/bioconf/20237101102.
Full textAbstract:
Phytopathogenic fungi cause significant damage to agriculture, reducing or destroying the crop. There are several ways to control fungal diseases, such as the use of disease-resistant plant varieties, organic farming, and the use of fungicides. One of the methods of combating phytopathogens is the use of biological products, one of which is biosurfactants. Biosurfactants are surfactants produced in the course of life. They are able to reduce fungal growth and increase plant disease resistance, improve plant growth and yield, and act as adjuvants to improve wetting and solubility of fertilizers. In the course of this work, the fungicidal activity of biosurfactants formed by Bacillus mojavensis PS17 was assessed against pathogens of the genera Fusarium and Verticillium. It has been established that the biosurfactant is able to reduce the coverage area of Fusarium and Verticillium fungi in in vitro experiments by 39 and 43%. Thus, it can be concluded that the biosurfactant produced by Bacillus mojavensis PS17 has antifungal activity.
38
Maherani, Vincentia Fenice Angger, Nisa Rachmania Mubarik, Jepri Agung Priyanto, and Ivan Permana Putra. "Biosurfactant Activity of Bacillus sp. Strain LP04 Isolate and Its Antifungal Potency against Ganoderma boninense and Fusarium sp." HAYATI Journal of Biosciences 31, no. 4 (April 1, 2024): 725–32. http://dx.doi.org/10.4308/hjb.31.4.725-732.
Full textAbstract:
Biosurfactants are a class of amphipathic molecules that various microorganisms can produce. Biosurfactants are used as biopesticides and biocontrol agents because they have antimicrobial activity, especially as antifungal agents in several species of fungal pathogens such as Ganoderma boninense and Fusarium sp. that attack crops. This study aims to detect the biosurfactant activity of Bacillus sp. and its potential as an antifungal agent against the fungi Ganoderma boninense and Fusarium sp. Biosurfactants were produced in mineral salt medium (MSM) by harvesting cell-free supernatants. Screening of biosurfactant-producing isolates was carried out using an oil-spreading assay, a hemolysis assay, and an emulsification index. The antifungal activity of the isolates was then tested using the agar diffusion method. The LP04 isolate was closely related to Bacillus thuringiensis with a 99% similarity level. It has the potential to have biosurfactant activity, which is characterized by a positive result on the oil spreading assay test and has an emulsification index of 48.33±2.87%. The cell-free supernatants of the bacterial isolate were able to inhibit the growth of Ganoderma boninense and Fusarium sp. with growth inhibition rates of 51.11% and 56.92%, respectively.
39
Vučurović, Damjan, Bojana Bajić, Zorana Trivunović, Jelena Dodić, Marko Zeljko, Rada Jevtić-Mučibabić, and Siniša Dodić. "Biotechnological Utilization of Agro-Industrial Residues and By-Products—Sustainable Production of Biosurfactants." Foods 13, no. 5 (February 26, 2024): 711. http://dx.doi.org/10.3390/foods13050711.
Full textAbstract:
The importance and interest in the efficient use and valorization of agro-industrial residues and by-products have grown due to environmental problems associated with improper disposal. Biotechnological production processes, including microbial biosurfactant production, represent a sustainable way to utilize agro-industrial residues and by-products, which are applied as substrates in these processes. Biosurfactants produced by microorganisms using renewable resources are a viable alternative to traditional petrochemical surfactants and have several potential uses in a wide range of industrial sectors due to their minimal ecotoxicity, easy biodegradability, and moderate production conditions. The common applications of biosurfactants, besides in food industry as food additives and preservatives, are in agriculture, environmental protection, the cosmetics and pharmaceutical industry, wastewater treatment, the petroleum industry, etc. This review aims to summarize the comprehensive scientific research related to the use of various agro-industrial residues and by-products in the microbial production of biosurfactants, as well as to emphasize the present state and the importance of their sustainable production. Additionally, based on the available biosurfactant market analysis datasets and research studies, the current situation in science and industry and the future perspectives of microbial biosurfactant production have been discussed.
40
Oyedeji, Olaoluwa, Deborah Ifeoluwa Onifade, and Anthony Abiodun Onilude. "Production, Characterization, and Application of Biosurfactant From Lactobacillus plantarum OG8 Isolated From Fermenting Maize (Zea Mays) Slurry." Acta Universitatis Cibiniensis. Series E: Food Technology 26, no. 2 (December 1, 2022): 271–86. http://dx.doi.org/10.2478/aucft-2022-0022.
Full textAbstract:
Abstract Biosurfactants have wide applications in several industries. However, high production costs and safety concerns have limited their comprehensive use. Twenty-five strains of lactic acid bacteria, isolated from fermenting maize slurry, were screened for biosurfactant production using the emulsification activity (E24) assay. The selected bacterium was identified molecularly using the 16S rRNA gene sequencing as Lactobacillus plantarum OG8. The effect of some cultural factors on biosurfactant production from the bacterium, using pineapple peel as a low-cost substrate, was investigated. The optimum yield of biosurfactant occurred at a 48 h incubation period, using glucose and peptone as carbon and nitrogen sources, respectively. The biosurfactant was characterized to possess mostly carbohydrates, followed by protein and lipid contents. Optima pH 10.0 and temperature 60 °C were the best for the biosurfactant activity. The biosurfactant exhibited antimicrobial activity against bacterial pathogens Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae, at a concentration of 5.0 mg/mL. The use of pineapple peel as a low-cost substrate for biosurfactant production from Lactobacillus plantarum OG8 will serve for cost-effective production. The biosurfactantt produced exhibited promising properties such as thermostability and antimicrobial activity against food spoilage and pathogenes that could make it suitable for food processing and preservation.
41
Yea, DaNan, SeonHui Jo, and JongChoo Lim. "Synthesis of Eco-friendly Nano-Structured Biosurfactants from Vegetable Oil Sources and Characterization of Their Interfacial Properties for Cosmetic Applications." MRS Advances 4, no. 07 (November 23, 2018): 377–84. http://dx.doi.org/10.1557/adv.2018.619.
Full textAbstract:
ABSTRACTIn this study, 3 types of zwitterionic phospholipid biosurfactants LDP(S), CDP(S) and CTDP(S) were prepared from 3 different raw materials such as rapeseed oil, coconut oil and cottonseed oil respectively. The structure of the resulting phospholipid biosurfactants was elucidated by FT-IR, 1H NMR and 13C NMR spectroscopies and their interfacial properties have been examined such as CMC, static surface tension, wetting property, solution stability, and foam property. Interfacial property measurement and prescription test in cosmetic formulation prepared with the newly synthesized biosurfactants revealed that CDP(S) biosurfactant possesses excellent mildness and superior interfacial properties, indicating the potential applicability in cosmetic product formulations.
42
Kaundal, Tamanna, Anjali Sharma, and Navneet Batra. "Optimization of Growth Parameters for Enhancing the Production of Biosurfactants from Pediococcus pentosaceus S-2." Biotechnology Journal International 27, no. 2 (May 11, 2023): 47–55. http://dx.doi.org/10.9734/bji/2023/v27i2678.
Full textAbstract:
Numerous Lactic acid bacteria (LAB) have been found to be capable of synthesizing surface-active compounds i.e biosurfactants. These are amphiphilic compounds produced by microorganisms on their cell surface or secreted extracellularly that have a tendency to reduce surface and interfacial tension. In the present study, different process parameters including nitrogen and carbon source, pH, temperature, aeration and agitation were optimized to maximize the production of biosurfactants from Pediococcus pentosaceus S-2. Xylose (1.5%) and yeast extract (1.5%) act as better carbon and nitrogen sources respectively for the production of biosurfactants. Maximum biosurfactant yield was observed at pH 6, a temperature of 35o C, an agitation rate of 200 rpm and with inoculum size of 3%. The high yield of biosurfactants produced from Pediococcus pentosaceus S-2 by utilizing media supplemented with whey under optimized conditions.
43
Stella, Nnabugwu Chidmma, Ekwealor, Chito Clare, Anaukwu, Chikodili Gladys, Anakwenze Vivian Nonyelum, Mba, Anthonia Nkiru, and Ekwealor, Ikechukwu Amaechi. "Isolation and Screening of Biosurfactant-Producing Bacteria from Hydrocarbon-Contaminated Soils in Awka, Southeast, Nigeria." Microbiology Research Journal International 33, no. 10 (December 6, 2023): 9–19. http://dx.doi.org/10.9734/mrji/2023/v33i101407.
Full textAbstract:
Background: Biosurfactants are a variety of groups of active compounds produced by various types of microorganisms. Bacterial surfactants are suitable for a range of applications in the oil industry, food, agriculture, pharmaceutical, cosmetics and bioremediation. Therefore, the search for sustainable alternatives to production of chemical biosurfactant is widely being encouraged.
Aim: The present study aimed to isolate and screen bacterial species for biosurfactant production.
Methodology: Two hundred soil samples collected from hydrocarbon contaminated soils in Awka, Nigeria, were cultured on casein starch agar. The isolates were screened for biosurfactant production stability and antimicrobial activity.
Results: Primary screening on the 87 recovered isolates using drop collapse, oil spreading, haemolysis tests showed that 16 isolates (18.39%) were biosurfactant producers. Secondary screening resulted in 9 isolates with good emulsification index (E24) ranging from 42.18% to 74.07%. The culture supernatant of the isolates containing biosurfactants were stable at varying pH (2 – 12), temperature (4 - 121˚C) and salinity (2 – 20%). Most isolates have antimicrobial activity against Escherichia coli and Staphylococcus aureus. Based on oil spreading, emulsification index and antimicrobial activity, four of the isolates, ASP, AS1, DS4, DS8, were selected as most efficient in biosurfactant production. Phylogenetic analysis based on 16S rRNA genes classified ASP as Streptomyces sp, AS1 as Actinomyces sp, DS4 and DS 8 as species of Alcaligenes faecalis.
Conclusion: All the species investigated were observed to be active biosurfactant producers. The stability of the biosurfactants, and their antimicrobial nature, make them suitable candidates for use in various industrial sectors.
44
Edosa, Tariku Tesfaye, Yong Hun Jo, Maryam Keshavarz, In Seon Kim, and Yeon Soo Han. "Biosurfactants Induce Antimicrobial Peptide Production through the Activation of TmSpatzles in Tenebrio molitor." International Journal of Molecular Sciences 21, no. 17 (August 24, 2020): 6090. http://dx.doi.org/10.3390/ijms21176090.
Full textAbstract:
Biosurfactant immunomodulatory activities in mammals, nematodes, and plants have been investigated. However, the immune activation property of biosurfactants in insects has not been reported. Therefore, here, we studied the defense response triggered by lipopeptides (fengycin and iturin A), glycolipids (rhamnolipid), and cyclic polypeptides (bacitracin) in the coleopteran insect, mealworm Tenebrio molitor. The in vitro antimicrobial activities against Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria and fungi (Candida albicans) were assessed by mixing these pathogens with the hemolymph of biosurfactant-immune-activated larvae. E. coli growth was remarkably inhibited by this hemolymph. The antimicrobial peptide (AMP) induction results also revealed that all biosurfactants tested induced several AMPs, exclusively in hemocytes. The survivability analysis of T. molitor larvae challenged by E. coli (106 CFU/µL) at 24 h post biosurfactant-immune activation showed that fengycin, iturin A, and rhamnopid significantly increased survivability against E. coli. Biosurfactant-induced TmSpatzles activation was also monitored, and the results showed that TmSpz3 and TmSpz-like were upregulated in the hemocytes of iturin A-injected larvae, while TmSpz4 and TmSpz6 were upregulated in the fat bodies of the fengycin-, iturin A-, and rhamnolipid-injected larvae. Overall, these results suggest that lipopeptide and glycolipid biosurfactants induce the expression of AMPs in T. molitor via the activation of spätzle genes, thereby increasing the survivability of T. molitor against E. coli.
45
Bharali, Pranjal, Salam Pradeep Singh, Yasir Bashir, Nipu Dutta, Bolin Kumar Konwar, and Chingakham Brajakishor Singh. "Characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria: potential application in bioremediation." Nova Biotechnologica et Chimica 17, no. 2 (December 1, 2018): 103–14. http://dx.doi.org/10.2478/nbec-2018-0011.
Full textAbstract:
Abstract Petroleum and hydrocarbons contamination can be remediated by physical, chemical or biological methods. Among these, in situ bioremediation is considered to be environmentally friendly because it restores the soil structure, requires less energy input and involves the notable removal after degradation of biosurfactant. The present study involves the characterization and assessment of biosurfactant producing indigenous hydrocarbonoclastic bacteria and their potential application in bioremediation processes. Three bacterial strains were isolated from various crude oil contaminated environments and characterized using standard identification techniques. The results clearly demonstrate the capability of utilizing hydrocarbon and biosurfactant produced by the bacterial strains. 16S rDNA sequencing followed by BLAST analysis revealed their similarity to Pseudomonas aeruginosa. The physico-chemical characterization of the biosurfactants revealed significant surface properties with stability at extreme temperature conditions (up to 121˚C), pH (5 - 8) and salinity (up to 4 %). Further, the mass spectrometry confirmed predominance of di-rhamnolipids in biosurfactant mixtures. The biosurfactants were found to be efficient in the removal of crude oil from the contaminated sand suggesting its applicability in bioremediation technology. Further, improved discharge of crude oil at elevated temperatures also confirms their thermo-stability which, could be exploited in microbial enhanced oil recovery processes. Thus, the applications of biosurfactants produced by the indigenous hydrocarbonoclastic strains appeared to be advantageous for bioremediation of petroleum-contaminated environments.
46
Suryanti, Venty, Sri Hastuti, Tutik Dwi Wahyuningsih, Mudasir Mudasir, and Dina Ika Muliawati. "BIOSURFACTANTS PRODUCTION BY Pseudomonas aeruginosa USING SOYBEAN OIL AS SUBSTRATE." Indonesian Journal of Chemistry 9, no. 1 (June 20, 2010): 107–12. http://dx.doi.org/10.22146/ijc.21570.
Full textAbstract:
Optimization condition of the biosurfactants production by P. aeruginosa using soybean oil as substrate has been examined. The media containing 10% v/v of the soybean oil and 6 days of the fermentation time was the optimum condition for the biosurfactants production. The extraction technique using different solvent polarity (n-hexane, chloroform, ethyl acetate and buthanol, respectively) was applied for the isolation of the biosurfactants. The biosurfactant was found in the extract chloroform of the crude biospasoy (biosurfactants obtained from soybean oil as substrate) which then is called chlo-biospasoy. The chlo-biospasoy was identified as rhamnolipids which had oil in water (o/w) emulsion type, had the CMC of 860 mg/L and could reduced the surface tension of the water from 72 mN/m to 52 mN/m. The chlo-biospasoy could be used as an emulsifier to form emulsion between water and hydrocarbon such as palm oil, benzene, premium or toluene with various stability. The results indicated that chlo-biospasoy could be used as an emulsifying and emulsion-stabilizing agent. Keywords: Biosurfactants, P. aeruginosa, Soybean Oil, Emulsifier
47
M.B. Muhsin, Yusra, Huda Z. Majeed, Basam Basim Mohammed, and Salih A.A. Mohammed. "Lactic Acid Bacteria Biosurfactant Role That Isolated from Human Breast Milk in Inhibit Eyes Pathogenic Bacteria." Ibn AL- Haitham Journal For Pure and Applied Science 31, no. 2 (September 12, 2018): 31. http://dx.doi.org/10.30526/31.2.1959.
Full textAbstract:
Biosurfactants have a wide-range of applications due to their unique properties like specificity, not toxicity (from LAB) and relative ease of preparation. These properties hold promise of biosurfactants to increase breast milk benefit were isolated and described into Lactobacillus plantarum, Lactobacillus fermentum ,Lactococcuslactis, and Leuconostocmesenteroides.The degree of microbial destruction of disease, which promotes the effective remediation of disease spreading.This paper presents a review of available research, methods and publications regarding Biosurfactant extraction from Lactic Acid bacteria isolated from human breast milk. 3 samples of human breast milk was provided, LAB were isolated and described, Biosurfactants recovery and surface activity were tested and extracted endo and extra cellular. In other side 26 samples from eye patients were ordered, diagnosed and their sensitivity to biosurfactant were studied. The results showed that 5 isolates of LAB from human breast milk were biosurfactant producer but L. plantarum was the more efficiency in surface activity. In other side, out of 26 eyes sample 18 were positive to pathogenic bacteria included E.coli (7), Klebseilla (5), Staphylococcus aureus(3)and S.epidermides (3).Extracellular Biosurfactanthad good effect against tested bacteria, but endocellular (extraction by normal method) had not any effect against any bacteria, whereas by solvents method were the more active against all tested bacteria. The results are promising enough to continue the quest for enhancement of inhibition growth of pathogenic bacteria with biosurfactant application (extracted extracellular by solvents) to look forward for biosurfactant as a solution of antibiotic resistance problem. In this study we concluded thatL. plantarum was the more effectiveness in biosurfactant surface activity and the extracellular biosurfactant by solvent method for extraction were better than endocellular and normal method of extraction.
48
Kuczajowska-Zadrożna, Małgorzata, Urszula Filipkowska, and Tomasz Jóźwiak. "Application of biosurfactants for heavy netals leaching from immobilized activated sludge / Zastosowanie biosurfaktantów do wymywania metali ciężkich z immobilizowanego osadu czynnego." Archives of Environmental Protection 41, no. 1 (March 1, 2015): 43–52. http://dx.doi.org/10.1515/aep-2015-0006.
Full textAbstract:
Abstract This study was undertaken to determine the effectiveness of biosurfactants - saponin, tannin and rhamnolipids JBR 515 and 425, for the removal of cadmium, zinc and copper from activated sludge immobilized in 1.5% sodium alginate with 0.5% polyvinyl alcohol. We also established the impact of pH value on biosorbent regeneration with the analyzed biosurfactants and determined the critical micelle concentration (CMC) in solutions containing the biosorbent and biosurfactant and in exact samples with heavy metals. Saponin exhibited the highest effectiveness of metals leaching at pH 1-5, and rhamnosides at pH 5-6. In addition, the study demonstrated a significant effect of the ratio of biosorbent mass to washing agent volume (m/V) on the effectiveness of metals leaching. Of the biosurfactants analyzed, saponin was ca. 100% effective in leaching zinc and copper. The effectiveness of the other biosurfactants was lower and depended on the metal being leached
49
Onlamool, Theerawat, Atipan Saimmai, and Suppasil Maneerat. "Antifungal Activity of Rhamnolipid Biosurfactant Produced by Pseudomonas aeruginosa A4 against Plant Pathogenic Fungi." Trends in Sciences 20, no. 3 (December 28, 2022): 6524. http://dx.doi.org/10.48048/tis.2023.6524.
Full textAbstract:
Biosurfactants have been shown to have a variety of other agricultural applications. Antimicrobial activity is a desirable property in a variety of biosurfactants. Several biosurfactants produced from bacteria have antibacterial activity against plant diseases, making them a promising biocontrol molecule for ensuring agricultural sustainability in the long run. The purpose of this research was to characterize biosurfactant produced at different carbon source by biosurfactant-producing bacterial strains isolated from contaminated soapstock sediment, as well as its antifungal properties. Biosurfactant-producing bacteria were isolated from soapstock-contaminated soil which bacteria were selected by using drop collapse and oil displacement tests. Twelve isolates reduced surface tension of culture broth from 50 to 31 - 43 mN/m. According to 16S rRNA sequence analysis, these isolates belong to 8 different genera (Acinetobacter, Citrobacter, Enterobacter, Klebsiella, Pantoea, Pseudomonas, Stenotrophomonas and Xanthomonas). The supernatant of Pseudomonas aeruginosa A4 grown in MSM supplemented with soapstock was the most effective biosurfactant against Aspergillus flavus F2, Aspergillus niger F14, Cunninghamella bertholletiae F1 and Rhizopus oryzae F5, inhibiting mycelium growth by 54, 61 59 and 50 %, respectively. The extract substance inhibited spore germination against A. flavus F2 and R. oryzae F5 with a minimum inhibitory concentration of 2.75 mg/mL. TLC, FT-IR, ESI-MS and GC-MS analysis demonstrated that the mono-rhamnolipids and di-rhamnolipids had the same 3-hydroxy fatty acid composition of C8, C10 and C12. Overall, the biosurfactant-producing strains identified in this investigation show promise for future development and application in cost-effective industrial-scale biotechnological processes.
HIGHLIGHTS
Biosurfactant producing bacteria were isolated from soapstock contaminated soil
The supernatant of Pseudomonas aeruginosa A4 grown in MSM supplemented with soapstock was the most effective biosurfactant against plant pathogenic fungi
Biosurfactant from Pseudomonas aeruginosa A4 was identified as rhamnolipid comprising of both mono and di-rhamnolipid congeners
GRAPHICAL ABSTRACT
50
Suryanti, Venty, Sri Hastuti, Tutik Dwi Wahyuningsih, Mudasir Mudasir, Dian Kresnadipayana, and Inge Wiratna. "Heavy Metal Removal from Aqueous Solution Using Biosurfactants Produced by Pseudomonas aeruginosa with Corn Oil as Substrate." Indonesian Journal of Chemistry 18, no. 3 (August 30, 2018): 472. http://dx.doi.org/10.22146/ijc.28805.
Full textAbstract:
The batch removal of Cu(II), Cd(II) and Pb(II) from individual heavy metal ion aqueous synthetic solution using biosurfactants produced by Pseudomonas aeruginosa with corn oil as substrate was investigated. The metal ion removal process of crude preparation biosurfactants (CPB) was established to be dependent on the initial pH and contact time. The optimum metal removal was observed at pH 6.0 of the initial metal solution and 10 min of contact time. The affinity sequence for metal ion removal was Pb(II)>Cd(II)>Cu(II). The removal capacity value of biosurfactant for Cu(II), Cd(II) and Pb(II) from single metal ions solution were 0.169, 0.276 and 0.323 mg/g, respectively. The removal capacity value of biosurfactant for Cu(II), Cd(II) and Pb(II) from multi metal ions solution were 0.064, 0.215 and 0.275 mg/g, respectively. The removal capacity of individual metal ion was diminished by the presence of other metal ions in multi metal ions from synthetic aqueous solution. The removal capacity value of biosurfactant for Cu(II), Cd(II) and Pb(II) from silver industry wastewater were 0.027, 0.055 and 0.291 mg/g, respectively. The results indicated that biosurfactants have potential to be used in the remediation of heavy metals in industrial wastewater.