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Dissertations / Theses on the topic 'Biotin-Streptavidin'

Author: Grafiati

Published: 4 June 2021

Last updated: 6 February 2022

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1

Chu, Vano. "Molecular recognition in the streptavidin-biotin system /." Thesis, Connect to this title online; UW restricted, 1998. http://hdl.handle.net/1773/8106.

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2

Hamblett, Kevin James. "Optimization of pretargeted radioimmunotherapy /." Thesis, Connect to this title online; UW restricted, 2002. http://hdl.handle.net/1773/8077.

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3

Sedlak, Steffen Matthias [Verfasser], and Hermann [Akademischer Betreuer] Gaub. "Mechanics of the streptavidin/biotin interaction / Steffen Matthias Sedlak ; Betreuer: Hermann Gaub." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2019. http://d-nb.info/1218970901/34.

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4

Moore, Adam. "On biomolecular interactions : investigating receptor-ligand interactions; theoretical and experimental approaches." Thesis, University of Nottingham, 1999. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.298073.

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5

Domingo, Rommel J. "Pre-targeted radioimmunotherapy with streptavidin-CC49 monoclonal antibody and §9§0Y-DOTA-biotin." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1997. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp01/MQ29337.pdf.

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6

Shimoboji, Tsuyoshi. "Photo-switching of protein activities by conjugation of photo-responsive polymers to proteins /." Thesis, Connect to this title online; UW restricted, 2001. http://hdl.handle.net/1773/8097.

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7

Schendel, Leonard C. [Verfasser], and Hermann [Akademischer Betreuer] Gaub. "Tether and reinforcement effects on Streptavidin-Biotin, and induced binding in nanoapertures / Leonard C. Schendel ; Betreuer: Hermann Gaub." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2020. http://d-nb.info/1214593380/34.

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8

Shea, Jessica Anna-Marie. "Streptavidin-biotin binding of DNA amplicons: methods for the typing and re-typing of forensically relevant short tandem repeats." Thesis, Boston University, 2012. https://hdl.handle.net/2144/12621.

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Thesis (M.S.)--Boston University<br>Submission of evidentiary samples to DNA units for exhaustive testing is becoming commonplace. For these samples, only one attempt at amplification is possible. However, more than one amplification may be necessary if the condition of the DNA causes poor amplification, more than one type of STR kit testing is required, or if there is an instrument malfunction during amplification. Current research into the re-amplification of already amplified samples focuses on placing the PCR product back into the thermal cycler with new reagents for additional cycles. The

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9

Luschtinetz, Franziska. "Cyaninfarbstoffe als Fluoreszenzsonden in biomimetischen und biologischen Systemen : Fluoreszenz-Korrelations-Spektroskopie und Fluoreszenzanisotropie-Untersuchungen." Phd thesis, Universität Potsdam, 2010. http://opus.kobv.de/ubp/volltexte/2010/4847/.

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Um Prozesse in biologischen Systemen auf molekularer Ebene zu untersuchen, haben sich vor allem fluoreszenzspektroskopische Methoden bewährt. Die Möglichkeit, einzelne Moleküle zu beobachten, hat zu einem deutlichen Fortschritt im Verständnis von elementaren biochemischen Prozessen geführt. Zu einer der bekanntesten Methoden der Einzelmolekülspektroskopie zählt die Fluoreszenz-Korrelations-Spektroskopie (FCS), mit deren Hilfe intramolekulare und diffusionsgesteuerte Prozesse in einem Zeitbereich von µs bis ms untersucht werden können. Durch die Verwendung von sog. Fluoreszenzsonden können Info

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10

Stephan, Milena. "Development of a Biomembrane Sensor Based on Reflectometry." Doctoral thesis, Niedersächsische Staats- und Universitätsbibliothek Göttingen, 2013. http://hdl.handle.net/11858/00-1735-0000-0001-BB7E-B.

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Membranproteine spielen eine wichtige Rolle in vielen biochemischen Prozessen der Zelle, wie zum Beispiel der Signaltransduktion, der Zelladhesion oder auch der Erkennung von Krankheitserregern. Viele dieser Proteine sind von Bedeutung für die Entwicklung neuer innovativer Medikamente. Somit hat auch die Entwicklung von Sensoren, die die Untersuchung von Membranproteinen in ihrer natürlichen Umgebung erlauben an Bedeutung gewonnen [1]. Thema dieser Doktorarbeit war die Entwicklung von Analysekonzepten die es ermöglichen unterschiedliche Aspekte von Membraninteraktionen zu untersuchen und zu qu

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11

Xu, Dongdong [Verfasser]. "Multiresponsive Synthetic Cells, Precise Protein Micropatterns and Streptavidin-Biotin Conjugates Using the Interaction Between Ni2+-NTA and His-tagged Proteins / Dongdong Xu." Mainz : Universitätsbibliothek der Johannes Gutenberg-Universität Mainz, 2020. http://d-nb.info/1224896688/34.

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12

Tiwari, Umadevi B. "Development of methods to determine the binding capacities of solid supports and improvement in immunoassay efficiency using dendrimer-modified beads." University of Cincinnati / OhioLINK, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=ucin1259077463.

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13

Chivers, Claire Elizabeth. "Investigating high-affinity non-covalent protein-ligand interaction via variants of streptavidin." Thesis, University of Oxford, 2011. http://ora.ox.ac.uk/objects/uuid:631c65ed-08d9-484e-a8df-309a4c95df45.

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The Streptomyces avidinii protein streptavidin binds the small molecule biotin (vitamin H / B₇) with extraordinary stability, resulting in the streptavidin-biotin interaction being one of the strongest non-covalent interactions known in nature (K<sub>d</sub> ~ 10<sup>-14</sup> M). The stable and rapid biotin-binding, together with high resistance to heat, pH and proteolysis, has given streptavidin huge utility, both in vivo and in vitro. Accordingly, streptavidin has become a widely used tool in many different biotechnological applications. Streptavidin has also been the subject of extensive r

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14

Blomstergren, Anna. "Strategies for de novo DNA sequencing." Doctoral thesis, KTH, Biotechnology, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-3649.

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<p>The development of improved sequencing technologies hasenabled the field of genomics to evolve. Handling andsequencing of large numbers of samples require an increasedlevel of automation in order to obtain high throughput andconsistent quality. Improved performance has lead to thesequencing of numerous microbial genomes and a few genomes fromhigher eukaryotes and the benefits of comparing sequences bothwithin and between species are now becoming apparent. Thisthesis describes both the development of automated purificationmethods for DNA, mainly sequencing products, and a comparativesequenci

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15

Bubeníková, Lucia. "Izolace bakteriální DNA z potravin s využitím magnetických nosičů." Master's thesis, Vysoké učení technické v Brně. Fakulta chemická, 2011. http://www.nusl.cz/ntk/nusl-216733.

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The aim of the work was the selective isolation of bacterial DNA with help of magnetic carriers covered by streptavidine (PGMA-NH2-STV, MPG® Streptavidin). Conditions of functionalisation of carriers using two biotinylated probes were optimized: the amount of carrier, the amount of probe, binding of biotinyled probe to streptavidine. Purified DNA Lactobacillus was used for hybridization. DNA binding to the probe (DNA/DNA hybridization) and nospecific adsorption of DNA to the carrier were tested. Target DNA eluted from the carrier was identified using PCR with primers R16-1 and LbLMA1-rev and w

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16

Gespers, Anastassja Leyla [Verfasser], Michael [Akademischer Betreuer] Groll, Michael [Gutachter] Groll, and Dirk [Gutachter] Weuster-Botz. "The Red Sea Brine Pools and the Biotin-Streptavidin Technology as Sources for Highly Stable and Selective Biocatalysts / Anastassja Leyla Gespers ; Gutachter: Michael Groll, Dirk Weuster-Botz ; Betreuer: Michael Groll." München : Universitätsbibliothek der TU München, 2018. http://d-nb.info/1196292744/34.

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17

Ahmed, Kawther Khalid. "Tumor cells surface-engineered with polymeric particles for use as cancer vaccines." Diss., University of Iowa, 2016. https://ir.uiowa.edu/etd/6026.

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Cancer is a group of diseases caused by aberrant continuously proliferating cells capable of metastasis. Despite significant advances in preventive, diagnostic and treatment measures, cancer is one of the major causes of death in the United States, second only to heart diseases. Main treatment approaches are surgery, radiotherapy, chemotherapy, and the recently expanding immunotherapeutic approaches. The main challenge in treating cancer is the ability of cancer cells to mutate and develop resistance to drug treatments therefore lowering the efficacy of chemotherapy in preventing metastatic tu

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18

Authimoolam, Sundar Prasanth. "STABILITY OF AFFINITY BASED LAYER-BY-LAYER POLYMERIC SELF-ASSEMBLIES FOR ORAL WOUND APPLICATIONS." UKnowledge, 2011. http://uknowledge.uky.edu/cme_etds/3.

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Oral mucositis is a painful and debilitating chronic inflammatory condition that can result from chemo and/or radiotherapy. While current treatment strategies which provide temporary relief exist, there is still an unmet clinical need for a robust long active barrier strategy which can simultaneously provide protection and release drug to enhance the wound healing response. It is proposed that an affinity based layer-by-layer self-assembled barrier administered as a series of mouth rinses can allow for wound specific drug delivery, providing an effective regenerative therapy. In this work, bio

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19

Wozniak, Katarzyna. "Fabrication of folic acid-containing biohybrid structures for the controlled uptake in cancer cells." Master's thesis, Universidade de Aveiro, 2016. http://hdl.handle.net/10773/17508.

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Mestrado em Ciência e Engenharia de Materiais<br>In this work, biohybrid structures (BHS) based on streptavidin and pentavalent biotinylated dendritic glycopolymer were fabricated through non-covalent streptavidin-biotin conjugation and further functionalized with the folic acid for the selective targeting to tumor cells. Characterization of the hydrodynamic size and zeta potential of the structures together with the cytotoxicity studies were performed to assess the physical properties and biocompatibility of the structures. It was shown that stable BHS with defined sizes can be obtaine

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20

Palestino, Escobedo Alma Gabriela. "Biosensing and light enhancement by means of biofunctionalized porous silicon devices." Montpellier 2, 2008. http://www.theses.fr/2008MON20046.

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21

Wu, Pei-Jung. "CELL SURFACE COATINGS FOR MAMMALIAN CELL-BASED THERAPEUTIC DELIVERY." UKnowledge, 2019. https://uknowledge.uky.edu/cme_etds/104.

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The cell plasma membrane is an interactive interface playing an important role in regulating cell-to-cell, cell-to-tissue contact, and cell-to-environment responses. This environment-responsive phospholipid layer consisting of multiple dynamically balanced macromolecules, such as membrane proteins, carbohydrate and lipids, is regarded as a promising platform for various surface engineering strategies. Through different chemical modification routes, we are able to incorporate various artificial materials into the cell surface for biomedical applications in small molecule and cellular therapeuti

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22

Urbánková, Kateřina. "Elektrochemická charakterizace nanostrukturovaných povrchů modifikovaných biolátkami s thiolovou vazbou." Master's thesis, Vysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií, 2014. http://www.nusl.cz/ntk/nusl-220870.

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This master thesis deals with nanotechnology, nanoparticles and nanostructured surfaces, electrochemical methods, especially voltammetry, cyclic voltammetry, electrochemical impedance spectroscopy and contact angle measurement. One part is focused on electrodes primarily nanostructured and modified by substancies with thiol bound. Tutorial for preparation of gold nanostructured electrods is introduced in practical section including SEM photos of electrode surface. Nanostructured and bare gold electrodes were modified by 11-mercaptoundecanoic acid, streptavidin, glycine and biotin and measured

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23

Ness, Stanley J. "Functionalization of In-plane Photonic Microcantilever Arrays for Biosensing Applications." BYU ScholarsArchive, 2012. https://scholarsarchive.byu.edu/etd/3281.

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Microcantilevers have been investigated as high sensitivity, label free biosensors for approximately 15 years. In nearly all cases, a thin gold film deposited on the microcantilevers is used as an intermediate attachment layer because of the convenience of thiol-gold chemistry. Unfortunately, this attachment chemistry can be unstable when used with complex sample media such as blood plasma. The Nordin group at BYU has recently developed an all-silicon in-plane photonic microcantilever (PMCL) technology to serve as a platform for label-free biosensing. It has the advantage of being readily scal

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24

Nilebäck, Erik. "A novel biotinylated surface designed for QCM-D applications." Thesis, Linköping University, Department of Physics, Chemistry and Biology, 2009. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-19250.

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<p> </p><p>Control of protein immobilization at sensor surfaces is of great interest within various scientific fields, since it enables studies of specific biomolecular interactions. To achieve this, one must be able to immobilize proteins with retained native structure, while minimizing non-specific protein binding. The high affinity interaction between streptavidin (SA) and biotin is extensively used as a linker between a surface, where SA is immobilized, and the (biotinylated) molecule of interest. Self- assembled monolayers (SAMs) of poly- and oligo ethylene glycol (PEG and OEG) derivative

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25

Zuo, Shusheng. "Quantitation, Purification and Reconstitution of the Red Blood Cell Glucose Transporter GLUT1." Doctoral thesis, Uppsala : Acta Universitatis Upsaliensis : Univ-bibl. [distributör], 2005. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-5727.

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26

Gottschalk, Ingo. "Chromatographic Studies of Solute Interactions with Immobilized Red Blood Cells and Biomembranes." Doctoral thesis, Uppsala University, Department of Biochemistry, 2002. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-2668.

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<p>Specific and non-specific interactions of solutes with immobilized biomembranes were studied using chromatographic methods. Liposomes, proteoliposomes and red blood cell (RBC) membrane vesicles were immobilized by a freeze-thawing procedure, whereas whole RBCs were adsorbed in the gel beds using electrostatic interaction, binding to wheat germ agglutinin (WGA) or the streptavidin-biotin interaction. </p><p>Superporous agarose gel with coupled WGA was the most promising matrix for RBC adsorption and allowed frontal chromatographic analyses of the cells for about one week. Dissociation consta

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27

Lagerquist, Hägglund Christine. "Affinity-, Partition- and Permeability Properties of the Human Red Blood Cell Membrane and Biomembrane Models, with Emphasis on the GLUT1 Glucose Transporter." Doctoral thesis, Uppsala University, Department of Biochemistry, 2003. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3525.

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<p>The human glucose transporter GLUT1 is abundant in red blood cells, the blood-brain barrier and epithelial cells, where it mediates the transport of the energy metabolite, glucose. In the present work some properties of GLUT1, including affinity binding of both substrates and inhibitors, transport rates as well as permeabilities of aromatic amino acids and drug-membrane interactions were analyzed by chromatographic methods.</p><p>Reconstitution by size-exclusion chromatography on Superdex 75 from a detergent with a low CMC that provides monomeric GLUT1 was examined regarding D-glucose- and

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28

Wu, Chia-Lin, and 吳佳霖. "Target-Activated Streptavidin-Biotin Control Binding Probes for Microarray." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/b474ug.

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29

Chien, Wan-Ching, and 簡婉菁. "Superoxide Anion Detection and Imaging by Streptavidin-Biotin Controlled Binding Probes." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/vxn9gv.

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30

Lin, Yu-Sheng, and 林于聖. "Selective Modification of Biotin on Silicon Nanodevices via Localized Joule Heating for Streptavidin Detection." Thesis, 2011. http://ndltd.ncl.edu.tw/handle/23553766162036975378.

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碩士<br>國立交通大學<br>材料科學與工程學系奈米科技碩博士班<br>100<br>In this research, we report the experimental results of selective modification of biotin on silicon nanodevices for streptavidin real-time detection. First, we used localized Joule heating to ablate poly(methyl methacrylate) (PMMA) and generate a template at the lightly doped region (sensing region) on silicon nanodevice channel. The rapid and self-aligned ablation of PMMA via a single electrical pulse (ca. 5 µs) can be achieved. This localized Joule heating was also demonstrated to anneal gold film and converted it into gold nanoparticles in 50µs. O

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31

Chew, Chee-Ying, and 趙芷瑩. "Organelle-Specific Imaging Using Near-Infrared Fluorescence Switchable Merocyanine Dye and Biotin-Streptavidin Chemistry." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/bpen98.

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32

Chung, Tzu-Hsuan, and 鍾紫宣. "Enzyme-Catalyzed Fluorescence Signal Amplification and Cell-Imaging with Streptavidin-Biotin Controlled Binding Probe." Thesis, 2018. http://ndltd.ncl.edu.tw/handle/23rt7n.

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33

Ding, Jiun-Der, and 丁俊德. "Forster Resonance Energy Transfer (FRET) Analysis on Quantum Dot Surface Bioconjugation Using a Quantitative Streptavidin-Biotin." Thesis, 2005. http://ndltd.ncl.edu.tw/handle/50546217489527130292.

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碩士<br>國立陽明大學<br>生醫光電工程研究所<br>94<br>In recent years, cellular transport relative peptides have been conjugated on nanoparticles (NP) to investigate target delivery. The number of these bio-molecules, such as nuclear express sequence (NES), nuclear localize sequence (NLS), and cell-penetrating peptide, binding on a nano-particle is expected to affect the transport efficiency of the NP. Assuming that there exists an optimal number of peptides (binding on the NP) that maximizes the transport efficiency, it is important to find a method to determine the number of peptides binding on a NP, and to su

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34

Gespers, (Akal) Anastassja. "Engineering of novel Biocatalysts with Functionalities beyond Nature." Diss., 2019. http://hdl.handle.net/10754/630912.

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Novel biocatalysts are highly demanded in the white biotechnology. Hence, the development of highly stable and enantioselective biocatalysts with novel functionalities is an ongoing research topic. Here, an osmium ligating single-site ArM was created based on the biotinstreptavidin technology for the dihydroxylation of olefins. For the creation of the artificial catalytic metal center in the streptavidin (SAV) cavity, efficient osmium tetroxide (OsO4) chelating biotin-ligands were created. The unspecific metal binding of the host scaffold was diminished through genetical and chemical m

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35

Nababan, Robasa, and 駱巴莎. "Enhanced Upconversion Fluorescence of NaYF4:Yb3+,Tm3+ Nanocrystals with the Low Refractive Index Resonant Waveguide Grating Structure using Streptavidin–Biotin Affinity Binding as an Example." Thesis, 2017. http://ndltd.ncl.edu.tw/handle/ns2rrz.

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碩士<br>國立中正大學<br>物理系研究所<br>105<br>In this study, we investigate the enhancement effect of upconversion fluorescence (UCF) emission of low refractive index resonant waveguide grating (RWG) via streptaviin-biotin affinity binding to enhance upconversion luminescence of lanthanide-ion (Ln3+) doped upconversion nanoparticles (UCNP). We fabricate one dimensional grating structure consists low refractive index porous silica (n = 1.22) by using nanoimprint lithography process subsequent depositing of high refractive index thin film (TiO2) on the surface of the sample. By drop coating NaYF4:Yb3+,Tm3+ n

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36

Mohebali, Farzaneh. "Synthesis of a biotin-functionalized biguanide for the identification of the tumor growth inhibition mechanism of metformin." Thèse, 2017. http://hdl.handle.net/1866/21511.

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