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Journal articles on the topic 'Biuret test'

Author: Grafiati

Published: 2 June 2025

Last updated: 31 July 2025

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1

Hojjatie, Michael M., and Dean Abrams. "Validation for the Determination of Biuret in Water-Soluble, Urea-Based Commercial Inorganic Fertilizer Materials, Urea Solutions, and Sulfur-Coated Urea Products by Reversed-Phase Liquid Chromatography: Single-Laboratory Validation of an Extension of AOAC Official MethodSM 2003.14." Journal of AOAC INTERNATIONAL 97, no. 3 (2014): 712–20. http://dx.doi.org/10.5740/jaoacint.12-443.

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Abstract A single-laboratory validation (SLV) study for the LC determination of biuret in dry and liquid urea-based commercial fertilizers was conducted. A total of 23 samples were used: 11 commercial dry urea products, two urea ammonium nitrate products, eight liquid urea-based commercial fertilizers, and four sulfur-coated urea samples from different sources. In addition, one biuret standard from Aldrich and one sample from the Magruder check sample program were used as validation samples. The proposed method is an extension of AOAC Official MethodSM 2003.14 and is based on dissolution of th

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Meenakshi, Suresh, Kakade Kaushiki, and Khodade Khushi. "Biochemical test for identification of proteins by biuret reagent using microscale methods." i-manager's Journal on Life Sciences 3, no. 1 (2024): 6. http://dx.doi.org/10.26634/jls.3.1.20938.

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This research paper explores the qualitative analysis of protein samples using the Biuret reagent through microscale methods involving ceramic tiles, grooved tiles, and Eppendorf tubes. The study highlights the advantages of minimal reagent usage, reduced waste, and enhanced safety. The Biuret test is employed to detect peptide bonds indicative of protein presence. Protein concentrations used for the identity assay ranged from 0.1% to 1.0%. Protein was detected by all three methods compared; however, the Eppendorf method detected proteins at a concentration of 0.3% and above. The ceramic and g

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Syauqi, Ahmad, Muhamad Fuadi, and Hari Santoso. "Comparative Study of References and Protein Quantifications Using Biuret-Spectrophotometric Method." Chimica et Natura Acta 6, no. 2 (2018): 42. http://dx.doi.org/10.24198/cna.v6.n2.19224.

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Comparison between the references and the quantification of biuret standard curves can be performed using statistical methods. The objective of this study was to select a method of protein quantification of the biuret-spectrophotometric based on the similar standard curve of the compound. The study used experimental methods in the laboratory and references from a comparative scheme of mean that has categories of statistics called academic and practical terms. Two standard curves were tested with the reference data. The academic way was performed with a comparison of 11.779-12.401% confidence,

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Rita Amini Warastuti, Vira Yuniar J. Paute, and Sri Masyita D. Jailani. "TEST THE CONTENT OF GLOBULIN, BIURET AND HEMOGLOBIN IN BLOOD IN ADOLESCENT WOMEN." Journal of Health, Technology and Science (JHTS) 2, no. 1 (2021): 51–59. http://dx.doi.org/10.47918/jhts.v2i1.213.

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Blood is a liquid found in humans and all high-level animals that serves to transmit substances and oxygen needed by the body's tissues, transport chemicals from metabolism, and also as a defense of the body against viruses or bacteria. This study aims to find out the content of globulin and biuret in the blood of adolescent women and identify hemoglobin levels contained in this. Method used in this study is qualitative research that is descriptive, with sampling techniques that use purposive sampling method. The results showed that in this globulin test aims to see the presence of

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Dr., Raja Kumar Parabathina* Varun Shinde Sakshi Bagul. "Production of γ-aminobutyric acid (GABA) from plant (Carrot and Radish) based Saccharides". International Journal of Pharmaceutical Sciences 3, № 5 (2025): 2317–23. https://doi.org/10.5281/zenodo.15411871.

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Gamma-Aminobutyric Acid (GABA) is a non-protein amino acid widely recognized for its beneficial effects on human health, including its roles in reducing anxiety, regulating blood pressure, and promoting neurological health. In this study, an attempt is made to produce GABA using plant-based substrates—specifically carrot (Daucus carota) and radish (Raphanus sativus)—through microbial fermentation involving lactic acid bacteria (LAB). The selection of these vegetables is based on their availability, nutritional value, and natural content of fermentable sugars, which are critical for

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Ali, Anwar G., and Carol J. Lovatt. "Winter Application of Low-biuret Urea to the Foliage of `Washington' Navel Orange Increased Yield." Journal of the American Society for Horticultural Science 119, no. 6 (1994): 1144–50. http://dx.doi.org/10.21273/jashs.119.6.1144.

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The objective of this study was to test whether a single winter prebloom foliar application of low-biuret urea would increase the yield of 30-year-old `Washington' navel orange trees [Citrus sinensis (L.) Osbeck] on Troyer citrange rootstock [C. sinensis `Washington' × Poncirus trifoliata (L.) Raf.]. All trees received a winter (November to January) soil application of urea (0.5 kg N/tree). Trees were maintained under irrigation or irrigation was withheld from 1 Oct. to 1 Mar. To determine the optimal time for foliar urea application, trees in both irrigation main plots received one applicatio

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Bhavsar, Falguni, Tanuja Babaleshwar, Divya Bobade, Arvind Done, Trushal Katke, and Vinay Gaikwad. "Detection of Carbohydrates, Fats, and Proteins in Foodstuffs." International Journal for Research in Applied Science and Engineering Technology 12, no. 12 (2024): 686–91. https://doi.org/10.22214/ijraset.2024.65876.

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Abstract: The detection of carbohydrates, fats, and proteins in foodstuffs is crucial for understanding their nutritional content and quality. This analysis involves the use of simple chemical tests that produce observable changes, such as color changes, indicating the presence of these macronutrients. Carbohydrates are detected using tests like the Benedict's test for reducing sugars and the iodine test for starch. Fats are identified by the grease spot test or Sudan III dye test, while proteins are detected through the Biuret test. These methods are cost-effective, easy to perform, and essen

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Putri Lestari, Ayu, Tuti Alawiyah, and Nur Hidayah. "PENGARUH PAPARAN LINEAR ALKALYBENZEN SULFONAT (LAS) TERHADAP KADAR PROTEIN TOTAL PADA IKAN NILA (Oreochromis niloticus) DENGAN METODE SPEKTROFOTOMETRI UV-VIS." Jurnal Medical Laboratory 1, no. 2 (2022): 38–48. http://dx.doi.org/10.57213/medlab.v1i2.128.

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Tilapia is a source of protein that is healthy and low in fat. Tilapia is a source of protein that is healthy and low in fat. Animal protein is found in fish, one of which is tilapia which has a protein content of more than 16.05%. One of the causes of protein denaturation is detergent. Razmi's research results, 2021 measuring the levels of laundry waste in Martapura river water in Sungai Lulut Village obtained concentrations at a distance of 100 meters of 3.4 mg/L, a distance of 200 meters of 2.4 mg/L and a distance of 300 meters of 1.6 mg/L. To determine the effect of exposure to Linear Alka

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Ozurumba, A. U., and P. C. Ohahuna. "Analysis of Protein Content of Hair Cream Enriched with Keratin Extract from Chicken Feathers." Science View Journal 3, no. 1 (2022): 174–77. http://dx.doi.org/10.55989/upfl3030.

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This work investigated the extraction of keratin from chicken feathers and the use of the extract in the production of hair cream, for a period of 2 weeks. The study is aimed at finding beneficial utilization of such wastes like chicken feathers in the improvement of beauty products. Standard chemical procedure for keratin extraction was used; pretreatment and dissolution of feathers, protein precipitation and protein purification. The results obtained indicate that keratin was extracted from chicken feathers using different reducing agents. The keratin produced was 61.4% pure and was subjecte

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Thysell, Hans. "A COMPARISON BETWEEN ALBUSTIX, HEMA-COMBISTIX, LABSTIX, THE SULPHOSALICYLIC-ACID TEST, HELLER'S NITRIC-ACID TEST, AND A BIURET METHOD." Acta Medica Scandinavica 185, no. 1-6 (2009): 401–7. http://dx.doi.org/10.1111/j.0954-6820.1969.tb07355.x.

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Mafaz, Zhafira, Afra Majida Hariono, and Luthfi Nurhidayat. "Antiangiogenic Activity of Pineapple (Ananas comosus) Stem Extract on Chicken Embryo’s Chorioallantois Membrane (CAM)." BIO Web of Conferences 94 (2024): 02007. http://dx.doi.org/10.1051/bioconf/20249402007.

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Inhibition of angiogenesis is able to suppress cancer growth by starving the cancer cells. It has been reported that the growth of human tongue squamous cell carcinoma can be inhibited by administering pineapple’s (Ananas comosus) extract. However, antiangiogenic activity of this extract has not been studied yet. This study aimed to investigate antiangiogenic activity of pineapple’s stem extract on chorioallantoic membrane (CAM) of chicken embryo. Pineapple stems were extracted by ultrasonic-assisted method using ethanol 96%. The chemical compositions were determined by thin layer chromatograp

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Rabat, Nurul Ekmi, Shahrir Hashim, and Rohah A. Majid. "Effect of Oil Palm Empty Fruit Bunch-Grafted-Poly(Acrylic Acid-co-Acrylamide) Hydrogel Preparations on Plant Growth Performance." Key Engineering Materials 594-595 (December 2013): 236–39. http://dx.doi.org/10.4028/www.scientific.net/kem.594-595.236.

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Two series of oil palm empty fruit bunch-grafted-poly (acrylic acid-co-acrylamide) [OPEFB-g-P(AA-co-AA slow release fertilizer hydrogels (SRFHs) were prepared via two different techniques namely double dryings of hydrogel (T1) and in situ intercalated urea (T2). Fourier Transform Infrared (FTIR) spectroscopy spectra showed-OH of OPEFB successfully grafted within P(AA-co-AAm) in T1 and T2. Water retention test of red okra plant (abelmoschus escule) and plant growth of rose balsam plant (impatiens balsamina) had showed that no biuret production has occurred in T2 and is more convenient in term o

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Wahdania, Fitria Porodjia, and Srikit Nurkamiden. "PROTEIN TEST WITH CHEMICAL SOLUTION IN IDENTIFYING NUTRIENT CONTENT IN FOOD MATERIAL." Journal of Health, Technology and Science (JHTS) 2, no. 4 (2022): 41–49. http://dx.doi.org/10.47918/jhts.v2i4.252.

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This study aims to find out the four factors that because proteins can be denatured, Knowing the solubility of proteins, as well as Knowing the bond of peptides in proteins, the presence of free and aromatic amino. The method used in this study is qualitative research that is descriptive, with sampling techniques that use purposive sampling method.The results showed that observations of protein denaturation that aims to see the presence of deposits in milk and the results obtained that does not occur precipitation, deposition by metals as for the results obtained that is only raw egg whi

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Sulistiyawati, Sulistiyawati, and Adi Cahya. "Food Analysis Labwork Guide as A Learning Media." Proceeding International Conference on Science and Engineering 1 (October 31, 2017): 235–38. http://dx.doi.org/10.14421/icse.v1.308.

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This study aimed to produce practical guide labwork using a project-based learning models to know the quality of products and to produce practical guide that can be used as a learning media. The study consisted of a preliminary study in the form of carbohydrat tests such as: iodine test, seliwanoff's test and moore test. Lipid solubility test within alcohol, ether and chloroform. Protein tests: biuret test, test and xanthoprotein ninhydrin test. Vitamin C tests: benedict test and iodine test. The results of the research were developed into a practical guide with coreldraw x6 software applicati

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Ukam, N. U., J. N. Nwangwa, and E. E. Ekpenyong. "The Effect of Ethanolic Root Extract of Gongronema Latifolium on Total Plasma Protein, Albumin and Globulin Concentration." European Journal of Food Science and Technology 13, no. 1 (2025): 15–22. https://doi.org/10.37745/ejfst.2013/vol13n11522.

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Gongronema latifolium (Utazi), a tropical plant native to Nigeria, is traditionally used for both culinary and medicinal purposes. This study examined the effects of its ethanolic root extract on plasma protein, albumin, and globulin levels in Wistar rats. Twelve rats (140–160 g, both sexes) were randomly divided into control and test groups (n=6 each). The control group received standard feed, while the test group received 200 mg/kg body weight of the extract orally for 21 days, alongside standard feed. All animals had free access to water. Blood samples collected post-treatment were analyzed

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A. Kumaresan, A. Kumaresan, and P. Rajesh P. Rajesh. "Synthesis and Characterization of Fertilizer Grade Urea from Cattle Waste." Oriental Journal Of Chemistry 38, no. 2 (2022): 486–89. http://dx.doi.org/10.13005/ojc/380236.

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Nitrogen, Phosphorous and Potassium are the three major nutrients required for healthy plant growth. Cow urine contains significant amount of Nitrogen and Potassium. The recent research show that only 20% of nitrogenous material consumed by cattle is absorbed remaining 80% excreted in urine and dung. This study shows that the fertilizer grade urea synthesized from cow urine by standard procedure and characterized by FT-IR for functional group determination. The presence of urea in the sample was confirmed by Biuret test. This study gives a ample scope to the farmers to increases the soil ferti

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Rusita, Youstiana Dwi, Wynne Afra Shafira, and M. Ali Nasikin. "Physical Characteristics of Processed Food Meatballs Based on Mussels (Anadara granosa)." Journal of Experimental and Clinical Pharmacy (JECP) 3, no. 1 (2023): 61. http://dx.doi.org/10.52365/jecp.v3i1.621.

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Seafood is great for consumption by various age groups because it is rich in protein, fat, vitamins and minerals. One of the seafood that is often used by the community is shellfish. Mussels (Anadara granosa) is a type of shellfish commonly eaten by Asian people because it has economic value and excellent nutritional content for consumption, especially a source of protein and minerals. One of the processed forms of mussels is meatball. The purpose of the study was to determine the results of physical quality (organoleptic and pH), chemical quality (fat and protein) in mussels meatballs (Anadar

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18

Ejoba, Raphael. "Effect of Saccharum barberi on the total serum protein and serum albumin in Albino wistar rats and Guinea pigs." Biolife 4, no. 3 (2022): 503–6. https://doi.org/10.5281/zenodo.7330582.

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<strong>ABSTRACT</strong> The effect of <em>Saccharum barberi</em> on total serum protein and serum albumin in albino wistar rats and Guinea pigs were determined. An ethanolic extract of saccharum barberi was made. Twenty (20) Albino wistar rats and twenty Guinea pigs of both male and female were used for the experiment. The albino wistar rats and Guinea pigs were divided into four groups (A – D), each group made up of five (5) animals Group A is the control while B, C, D were the test animals. The test animal were given a dose level of 100mg/kg, 200mg/kg and 300mg/kg body weight respect

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MAHMUD, Aminu Dikko, Zeenat Aliyu, Bilqis Abdullahi Oluwatoyin, Aliyu Ahmed, Suleiman Yahaya A, and Aisha Mohammed Saleh. "Extraction and Characterization of Keratin Protein from Chicken Feathers using Alkaline Hydrolysis Method: Effects of Sodium Sulphide Concentration and Shelf-life Evaluation." Malaysian Journal of Catalysis 7, no. 2 (2023): 37–41. http://dx.doi.org/10.11113/mjcat.v7n2.173.

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This study investigated the extraction of keratin protein from chicken feathers through alkaline hydrolysis using sodium sulphide as a digesting agent. The protein was precipitated using hydrogen chloride and confirmed through biuret test, solubility test, sulfur test, and FTIR analysis. The effect of varying sodium sulphide concentrations (0.5 M, 0.75 M, and 1 M) on the extracted keratin was evaluated. Results showed that a higher concentration of sodium sulphide produced a higher yield of keratin, with 1 M producing 65.8% yield. However, the shelf-life of wet keratin extracted using 1 M conc

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Bhadarka, Nagajan, Krutika Poddar, and Satyam Joshi. "Utilization of urine protein/creatinine ratio in pregnancy for diagnosis of preeclampsia." International Journal of Reproduction, Contraception, Obstetrics and Gynecology 7, no. 9 (2018): 3646. http://dx.doi.org/10.18203/2320-1770.ijrcog20183769.

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Background: The aim of the present study was to evaluate the ability of the random urine P/C ratio to predict significant proteinuria, as well as to introduce a diagnostic test for preeclampsia that would avoid the inconvenience and time consumption of 24-hour urine protein collection.Methods: A total of 100 women who were pregnant were included in the study. For urine test, the urine was collected for 24 hours. The women patients provided the mid stream urine sample prior to the collection period. Biuret colorimetric assay was used to determine the total protein in the urine and the creatinin

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Fu, Ruimin, Hong Zhang, Wei Tang, Xue Yang, Ding Wang, and Wuling Chen. "Study on the effect of fengycin on the respiration and metabolic mechanism of Penicillium expansum." Materials Express 11, no. 12 (2021): 2047–51. http://dx.doi.org/10.1166/mex.2021.2114.

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This study investigate the inhibiting effect of fengycin on respiration and nutrient utilization of Penicillium expansum. The respiratory inhibition rate of the P. expansum was determined by the test of dissolved oxygen fengycin, The effect of fengycin treatment on the activity of P. expansum mitochondrial complex enzyme was detected by mitochondrial enzyme activity assay. The ability of fengycin treatment to P. expansum the utilization of total sugar and total protein was determined by DNS colorimetric method and biuret method. After fengycin treatments, the TCA pathway of respiratory metabol

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Kos, Ivan, Milena Jadrijević-Mladar, Ivan Butula, Mladen Biruš, Gordana Maravić-Vlahoviček, and Sanja Dabelić. "Synthesis, antibacterial and cytotoxic activity evaluation of hydroxyurea derivatives." Acta Pharmaceutica 63, no. 2 (2013): 175–91. http://dx.doi.org/10.2478/acph-2013-0014.

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5 Synthesis and biological evaluation of a series (N = 16) of cyclic and acyclic hydroxyurea derivatives, including benzotriazole-, isocyanuric acid- and biuret-containing compounds, are disclosed. 1-N-(benzyloxycarbamoyl)benzotriazole was used as a benzyloxyisocyanate donor, a useful intermediate in the preparation of substituted hydroxyurea. Antibacterial activities of synthesized hydroxyurea derivatives were tested on three E. coli strains, i.e., a strain susceptible to antibiotics, a strain resistant to macrolide antibiotics and a strain resistant to aminoglycoside antibiotics. Six compoun

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Akanksha, Dr. Puneet, Gurinder Singh, and Harpreet Kaur. "Effect of Indian Medicinal Plants Extract against Isolated Bacteria Common Responsible for Food Spoilage Microorganism." International Journal of Scientific Research in Science and Technology 12, no. 3 (2025): 360–67. https://doi.org/10.32628/ijsrst2512345.

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The ongoing quest for novel antimicrobial agents to combat the threat posed by resistant microbes is essential to the effectiveness of food quality control. Using the disc diffusion method, the ethanol extracts of five medicinal spices (spinach, coriander, orange peel, fenugreek, and turmeric) that are used in Indian homes as flavor enhancers were examined for in vitro antibacterial activity against Salmonella typhi, a foodborne pathogen. Among all the examined bacterial isolates, ethanol extracts of coriander exhibited the strongest antibacterial activity (55 mm), whereas extracts of spinach

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Suardi, Sulfitri, Syaiful Bahri, Khairuddin, Ni Ketut Sumarni, and Erwin Abdul Rahim. "Perbandingan Kadar Albumin Ikan Gabus (Channa striata) dari Proses Perebusan dan Pengukusan dengan Menggunakan Uji Biuret." KOVALEN: Jurnal Riset Kimia 6, no. 1 (2020): 67–73. http://dx.doi.org/10.22487/kovalen.2020.v6.i1.12699.

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Cork Fish (Channa striata) is a freshwater fish that was spread throughout most of Indonesia and it contains albumin that was beneficial to the human body. Albumin was easily dissolved in water during the cooking process both boiled and steamed. This study aims to determine the influence of boiling and steaming times at 100oC against albumin content of cork fish that lost and left in fish meat. The times of boiling and steaming that was used to processing the fish were 10, 15, and 20 minutes. The albumin concentration that lost and left in for the boiling and the steaming process was determine

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Koroma, L., J. K. Senesie, F. W. Baion, B. M. Lukulay, and M. B. Kallon. "Synthesis Process and Nutritional Evaluation of Plantain as a Supplementary Food for Toddlers in Sierra Leone: A Study Utilizing Biuret’s, Benedict’s, and Iodine Solution Test." Spektra: Jurnal Fisika dan Aplikasinya 9, no. 2 (2024): 95–112. http://dx.doi.org/10.21009/spektra.092.04.

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Research on developing plantain fruit into juice, jam, ice cream, and fritters as a food supplement for toddlers was conducted in Sierra Leone. The work was done at the Mosoudo section of Daru village, Jawie Chiefdom, in Kailahun District. Four bunches of plantains were harvested from Jawei Chiefdom and stored in Mosoudo. Two bundles of the raw plantain were peeled, dried, and ground into powder, while the other two left to ripen at room temperature for nine days. Test were conducted using Iodine solution, Benedict solution, and Biuret test to check the presence of starch, glucose, and protein

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Panjaitan, Riong Seulina, Yulius Evan Christian, Bagas Eva Wijaya, and Rahmat Andrian. "Test Carbohydrate and Protein Content in Commercial Condensed Milk (SKM) Qualitative and Quantitative." Indonesian Journal of Pharmaceutical Research 3, no. 1 (2023): 31–45. http://dx.doi.org/10.31869/ijpr.v3i1.4606.

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Sweetened condensed milk (SKM) is a milk product obtained by evaporating some of the water from pure milk with the addition of sugar. SKM is not a sterile product, but its preservation depends on the sugar content; (1) Background: Qualitative and quantitative tests of carbohydrates and proteins were carried out to determine the presence of carbohydrates and proteins in SKM. The qualitative test is based on a change in color or precipitate formed, while the quantitative test is to determine carbohydrate and protein levels; (2) Method: Qualitative and quantitative test of carbohydrates with the

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Asysyifa, Rayyana Aurora Nur, Addina Rizky Fitriyanti, Hersanti Sulistyaningrum, and Agus Suyanto. "Kadar Protein, Kadar Albumin, dan Tingkat Kesukaan Brownies Mocaf Ikan Gabus." Jurnal Gizi 13, no. 1 (2024): 17. http://dx.doi.org/10.26714/jg.13.1.2024.17-29.

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Background: Brownies are generally made from wheat flour, but can be made with local food mocaf flour. Mocaf protein is lower than wheat flour, wheat flour has 8-13% protein while mocaf has 1.2% protein content. Objectives: The purpose of the study was to determine the addition of snakehead fish to protein levels, albumin levels, and preference test for mocaf brownies.The addition of snakehead fish to mocaf brownies aims to increase protein levels. Snakehead fish contains high protein and albumin,protein of 16.2g/100g and albumin content of 6.224g/100g. Preference test is researched to find ou

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Novotný, Ladislav, Anna Vachálková, and Alois Pískala. "Investigation of the Polarographic Properties and Potential Carcinogenity of Some Hydroxyurea Derivatives by DC Polarography." Collection of Czechoslovak Chemical Communications 61, no. 4 (1996): 656–62. http://dx.doi.org/10.1135/cccc19960656.

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Polarographic reduction was studied for a series of 7 urea derivatives and the results were used to assess their potential carcinogenity. The polarographic reduction was examined in absolutely anhydrous dimethylformamide by DC polarography. In the conditions applied, the majority of the compounds was reduced within a single two-electron step, only biuret and its formyl derivative were reduced in two one-electron steps. The potential carcinogenity of the substances was assessed based on the tg α value of the slope of dependence of the polarographic wave height on the concentration of α-lipoic a

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Agustina, Mulya, Rimbawan Rimbawan, Budi Setiawan, and Ainia Herminiati. "Pengaruh Pemberian Diet Rendah Protein dan Restriksi Pakan pada Pertumbuhan dan Protein Serum Tikus Lepas Sapih." Nutri-Sains: Jurnal Gizi, Pangan dan Aplikasinya 5, no. 1 (2021): 1–14. http://dx.doi.org/10.21580/ns.2021.5.1.4653.

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This study aimed to determine the effect of low protein diet and feed restriction on growth and serum protein. This experimental study using randomized controlled group design. Twelve male Sprague dawley (21-28 days old) weighing 50-100 grams were divided into three groups (4 rats/group): normal control, low protein diet and feed restriction group. The data analyzed by ANOVA test followed by Post Hoc Duncan. The determination of total protein using spectrophotometric biuret method and albumin was BCG method. The result showed that low protein diet 5% for 21 days occured weight loss (-5.25 ± 3.

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Elena V. Tsaregorodtseva, Tatyana V. Kabanova, Maria V. Dolgorukova, et al. "Effect of preparation of the test samples by dissolution on the indicators of the protein composition of animal meat." International Journal of Research in Pharmaceutical Sciences 11, no. 2 (2020): 2283–87. http://dx.doi.org/10.26452/ijrps.v11i2.2193.

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The article presents the research of the protein composition of meat of wild animals (elk and wild boar) and domestic animals (beef), by their isolation and separation methods due to their different solubility in different solutions, conducted in the laboratory of Biological Safety of Food Systems of the Department of Meat and Dairy Products Technology of Mari State University. The total proteins or protein fractions were extracted from a homogenized chilled meat sample. Then, the colorless proteins in the extract were transferred to the colored product, adding a biuret reagent, and studied th

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Elena V. Tsaregorodtseva, Tatyana V. Kabanova, Maria V. Dolgorukova, et al. "Effect of preparation of the test samples by dissolution on the indicators of the protein composition of animal meat." International Journal of Research in Pharmaceutical Sciences 11, no. 2 (2020): 2283–87. http://dx.doi.org/10.26452/ijrps.v11i2.2196.

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The article presents the research of the protein composition of meat of wild animals (elk and wild boar) and domestic animals (beef), by their isolation and separation methods due to their different solubility in different solutions, conducted in the laboratory of Biological Safety of Food Systems of the Department of Meat and Dairy Products Technology of Mari State University. The total proteins or protein fractions were extracted from a homogenized chilled meat sample. Then, the colorless proteins in the extract were transferred to the colored product, adding a biuret reagent, and studied th

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Rahayu, Lisa Eka Yuni, Deni Pranowo, and Tri Joko Raharjo. "Encapsulation of Lipase Enzyme in Silica Gel Matrix from Rice Husk Ash and Transesterification Reaction Activity Test on Palm Oil." Materials Science Forum 1067 (August 10, 2022): 177–83. http://dx.doi.org/10.4028/p-9d70d6.

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Encapsulation is one of the immobilization methods to increase the stability of the enzyme or other biomolecules by binding or trapping the molecules in a certain matrix. Silica gel was mostly chosen as a matrix to encapsulate the enzyme due to its high thermal stability. In this research, silica gel was used to encapsulate of lipase enzyme. Silica gel was synthesized from sodium silicate by the sol-gel technique. Silica from rice husk ash was extracted using 2 M NaOH to produce a sodium silicate solution. The silica gel was synthesized using the acidification of sodium silicate solution. The

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Almeida, Katyane de Sousa, Alinny Ferreira Costa, Paulo Cesar da Silva, José Jurandir Fagliari, Rosangela Zacarias Machado, and Adjair Antonio do Nascimento. "Acute phase proteins: a potential approach for diagnosing chronic infection by Trypanosoma vivax." Revista Brasileira de Parasitologia Veterinária 21, no. 2 (2012): 97–100. http://dx.doi.org/10.1590/s1984-29612012000200005.

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The present study aimed to assess potential changes in acute phase proteins in sheep experimentally infected with Trypanosoma vivax. There were studied eight male sheep, four used as controls and four infected with 10(5) T. vivax trypomastigotes. Blood samples were collected at two points times before infection and then at 5,7, 9, 11, 13, 15, 20, 30, 45, 60, 75, 90, 105 and 120 days post-infection (dpi). Blood samples were centrifuged and allotted, and acute phase proteins were then separated by electrophoresis on acrylamide gel containing sodium dodecyl sulfate. Protein concentrations were de

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Desi Sri Rejeki, Ery Nourika Alfiraza, and Eek Safita. "Penentuan Kadar Lemak Dan Protein Pada Daging Dan Biji Durian (Durio Zibethinus Murr.) Lokal Di Pasar Brebes." An-Najat 2, no. 3 (2024): 327–37. http://dx.doi.org/10.59841/an-najat.v2i3.1637.

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One of the tropical fruits that is rich in nutrients such as fat, protein, carbohydrates, vitamins and minerals is durian. Not a few people enjoy durian fruit, because it can be consumed by people of all ages. Consuming too much durian fruit can cause side effects such as high blood pressure, increased cholesterol levels, obesity and digestive system problems. This research aims to determine the fat and protein content in local durian flesh and seeds and compare the fat and protein levels. This research uses the soxhletation method and the biuret method with the help of UV-Vis spectrophotometr

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Dervishi, Aida, Merita Rumano, Panajota Ruzi, and Adriatik Çakalli. "The genetic diversity and variation in crude protein content of wheat (Triticum aestivum L.) promising cultivars for breeding in Albania." Agronomy Science 77, no. 3 (2022): 79–88. http://dx.doi.org/10.24326/as.2022.3.6.

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The genetic diversity and variation in crude protein content among eleven wheat genotypes, comprising three elite local genotypes and eight wheat genotypes of foreign origin were investigated in the present study. Variability was evidenced in grain protein content estimated by biuret test, it ranged from 9.5 to 13.9% with mean of 11.58%. Comparative analysis between local and introduced wheat genotypes revealed that the local genotypes had lower protein content than those of foreign origin. Fourteen polymorphic RAPD markers were used to assess genetic diversity among selected wheat varieties.

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Kholidah, Diniyah, Endy Paryanto Prawirohartono, and Fatma Zuhrotun Nisa. "Pemberian makanan F100 dengan bahan substitusi tepung tempe terhadap status protein pasien anak dengan gizi kurang." Jurnal Gizi Klinik Indonesia 10, no. 2 (2013): 92. http://dx.doi.org/10.22146/ijcn.18851.

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Background: Much effort has been made to counter the weaknesses of F100 through the development of milk powder substituted with ingredients that have a high content of protein and energy, high bioavailability and relatively low cost.Objective: To analyze an effect of F100 supplementation using substitute tempeh flour on protein status (total protein serum and serum albumin) among undernourished pediatric patients.Method: Experimental research with randomized clinical control trial among 30 undernourished children consisting of open clinical trial in two experiment groups, supplemented with F10

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Khyati, Patel, and R. Patel Falguni. "Screening of biosurfactant producing yeasts isolated from mangrove ecosystem of Surat region of Gujarat, India." Indian Journal of Science and Technology 13, no. 19 (2020): 1927–34. https://doi.org/10.17485/IJST/v13i19.204.

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Abstract <strong>Background/ Objectives:</strong> Biosurfactants are extracellular surface-active compounds produced by bacteria, fungi and yeast. Interest in biosurfactant has been increasing due to their unique properties. The objective of this study is to screen for potential biosurfactant producing yeasts from mangrove areas of Gujarat in India. <strong>Methodology:</strong> The biosurfactant producing yeasts isolated from five different mangrove sites i.e. Hazira, Mandroi, Mirzapur, Kantiyajal and Machhad of Surat, Gujarat during summer season of 2015. Isolated yeasts were

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Roy, Jenny Susan, Nandini Manjunath, Kishore Bhat, Anjana Mary George, Fathimath Nishana, and Lia Mathew. "Estimation of salivary glycoconjugates and salivary ros levels in chronic periodontitis: a clinico-biochemical study." International Journal of Research in Medical Sciences 5, no. 8 (2017): 3578. http://dx.doi.org/10.18203/2320-6012.ijrms20173566.

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Background: Periodontitis is a chronic inflammatory disease of periodontal tissue, characterized by persistent inflammation of the connective tissue and alveolar bone destruction. Patients with periodontal disease show the differences in the composition of saliva. Newer diagnostic tools based on analysis of body fluids such as saliva, GCF and serum are found to be useful for diagnosis as well as monitoring the disease activity. Thus, aim of the study was to estimate the concentration of salivary glycoconjugates (sialic acid, total protein) and salivary ROS in unstimulated whole saliva of subje

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Shabbir, Hasan, Konrad Wojtaszek, Bogdan Rutkowski, et al. "Milk-Derived Carbon Quantum Dots: Study of Biological and Chemical Properties Provides Evidence of Toxicity." Molecules 27, no. 24 (2022): 8728. http://dx.doi.org/10.3390/molecules27248728.

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Carbon dots (CDs) are carbon-based zero-dimensional nanomaterials that can be prepared from a number of organic precursors. In this research, they are prepared using fat-free UHT cow milk through the hydrothermal method. FTIR analysis shows C=O and C-H bond presence, as well as nitrogen-based bond like C-N, C=N and –NH2 presence in CDs, while the absorption spectra show the absorption band at 280 ± 3 nm. Next, the Biuret test was performed, with the results showing no presence of unreacted proteins in CDs. It can be said that all proteins are converted in CDs. Photo luminance spectra shows the

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As-Syauqi, Syarifah Miftahqillah, Indana Zulfa, Zakiyatun Nafsiyah, Muhammad Syahrul Rizki Okina, and Isnawati Isnawati. "Peanut (Arachis hypogaea) Milk Vegan Cheese with Pineapple (Ananas comosus) Bromelin Enzyme as Coagulant and Carrot (Daucus carota) Extract as Source of Antioxidants." International Journal of Biosciences and Biotechnology 11, no. 2 (2024): 25. http://dx.doi.org/10.24843/ijbb.2024.v11.i02.p04.

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Cheese has existed since ancient greece and became one of the fermented foods favored by the community. Cheese is obtained through the process of protein coagulation with the help of bacteria, enzymes, and acids. Increasingly accompanied by technological developments appear various types and preparations of cheese. Cheese is generally made from the milk of cows, goats, horses, and some other animals. But for the development of making cheese made from plants or plants is very rarely found. Making vegan cheese from peanut juice with coagulant enzyme, bromelin, pineapple and extra carrots as a so

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Onwuka, Ngozi Amanda, Atuboyedia Wolfe Obianime, and Kodichimma Serah Onwuka. "Impact of Gas Flaring on Forced Vital Capacity, Body Mass Index and Biochemical Parameters: Evidence from a Nigerian Oil-Bearing Community." International Journal of Research and Innovation in Applied Science X, no. I (2025): 468–81. https://doi.org/10.51584/ijrias.2025.1001037.

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The Nigerian economy experienced a leap with oil exploitation as oil accounted for over 70% of the country’s foreign exchange earnings. However, oil exploitation precipitated the problem of gas flaring. Consequently, this study seeks to evaluate the aftermath of gas flaring on the Forced Vital Capacity (FVC), Body Mass Index (BMI) and biochemical parameters of residents of Ibeno, an oil-bearing community in Akwa Ibom State, Nigeria. Afaha Udoe, another community in AkwaIbom State which is over 60km away from Ibeno, was used as control. Lung function was assessed using a spirometer while weight

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Rakhmah, Risza Fawzia, and Titik Suryani. "PEMANFAATAN BUAH LOKAL SEBAGAI KOAGULAN SOY CHEESE." Bioeksperimen: Jurnal Penelitian Biologi 2, no. 1 (2016): 8. http://dx.doi.org/10.23917/bioeksperimen.v2i1.1576.

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Keju merupakan salah satu produk pangan dengan proses penggumpalan protein susu. Penelitian ini memanfaatan buah lokal sebagai koagulan untuk menggumpalkan susu kedelai menjadi keju. Penelitian ini bertujuan untuk mengetahui pengaruh buah lokal sebagai koagulan pada soy cheese terhadap kadar protein, organoleptik dan daya terima masyarakat. Rancangan penelitian menggunakan Rancangan Acak Lengkap (RAL) 2 faktor 6 taraf perlakuan yaitu faktor 1: jenis buah (nanas, jeruk lemon dan asam sitrat (kontrol ) dan konsentrasi (0,12 %; 2%; 3 %), masing-masing perlakuan dilakukan 3 ulangan. Metode penelit

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Ibrahim, Dalal. "Statistical Study of Extract Keratin Protein from Waste Chicken Feather Based on Response Surface Methodology." Scientific Journal for the Faculty of Science-Sirte University 2, no. 2 (2022): 12–18. http://dx.doi.org/10.37375/sjfssu.v2i2.76.

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The feathers contain a significant amount of keratin protein, which is used in cosmetics, shampoos, hair treatment creams, and skin creams. Dissolving chicken feathers with reducing agent and then separating the protein from chemicals are the key steps involved. However, in order to enhance the amount of recovered keratin as much as possible, the best conditions for extracting keratin from chicken feathers are required. In this study, Response Surface Methodology (RSM) was used in order to simulate and optimize the operating parameters for extracting keratin from waste chicken feathers in orde

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Tkachenko, Halyna, Natalia Kurhaluk, and Irina Tkachova. "EXERCISE-INDUCED CHANGES IN THE LEVEL OF TOTAL PROTEIN AND ITS FRACTIONS IN THE BLOOD OF HORSES INVOLVED IN RECREATIONAL HORSEBACK RIDING." Scientific and Technical Bulletin of the Institute of Animal Science NAAS of Ukraine, no. 124 (2020): 35–46. http://dx.doi.org/10.32900/2312-8402-2020-124-35-46.

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The aim of the current study was to do the analysis of the total protein and its fraction in the blood samples of horses, which are involved in recreational horseback riding in the Pomeranian region (Pomeranian Voivodship, northern Poland). Thirteen healthy adult horses from the Pomeranian region in Poland (Strzelinko village, N54°30´48.0´´ E16°57´44.9´´), aged 9.5±2.4 years, including 5 Hucul ponies, 2 Thoroughbred horses, 2 Anglo-Arabian horses, and 4 horses of unknown breed, were used in the current study.Training started at 10:00 AM, lasted 1 hour, and consisted of a ride of cross country

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Anderson, J. W., and D. B. Jennings. "H+ homeostasis, osmolality, and body temperature during controlled NaCl and H2O intake." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology 255, no. 1 (1988): R97—R104. http://dx.doi.org/10.1152/ajpregu.1988.255.1.r97.

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Arterial PCO2, arterial [H+] ([H+]a), electrolytes, and osmolality, as well as rectal temperature (Tre), were monitored in six awake dogs over sequential 12- or 13-day periods in which their NaCl intake was first less than 5 meq/day, then approximately 120 meq/day, and finally less than 5 meq/day. Water intake was maintained constant at 77 ml.kg-1.day-1 throughout. During low-NaCl periods, decreases in body and plasma water, indicated by weight loss did not prevent lower arterial [Na+] ([Na+]a), arterial [Cl-] ([Cl-]a), and osmolality relative to the high-NaCl period. During high dietary NaCl,

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Basi-Chipalu, Shradha, Pallavi Sthapit, and Aastha Maharjan. "Isolation and identification of Bacillus species from soil and assessment of antimicrobial properties." Scientific World 16, no. 16 (2023): 77–84. http://dx.doi.org/10.3126/sw.v16i16.56819.

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The emergence of multi drug resistant pathogens such as Methicillin resistant Staphylococcus aureus (MRSA) has become a threat to public health. Thus, the development of new antibiotics has become a global concern. Many scientists are attracted towards discovery of new antimicrobial agents from microbial sources. The aim of this study is to isolate potential Bacillus species from soil for the production of antimicrobial substance. During the study, 20 soil samples were collected from various areas of Kathmandu valley. A total of 70 Bacillus isolates were identified by morphological and biochem

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Fauzi, Ahmad, Broto Santoso, Muhammad Haqqi Hidayatullah, Alifia Zafarani Azzuhra, and Azizah Ahsaninnisa. "FORMULATION AND EVALUATION OF GOLDEN SEA CUCUMBER (STICHOPUS HERRMANNI) ORAL SUSPENSION." Medical Sains : Jurnal Ilmiah Kefarmasian 10, no. 2 (2025): 137–44. https://doi.org/10.37874/ms.v10i2.1683.

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The Golden Sea Cucumber (Stichopus herrmanni) is widely recognized for its high protein and collagen content, making it valuable for medicinal applications, such as wound healing and tissue regeneration. The present study focused on formulating an oral suspension of sea cucumber extract, assessing its stability, and determining its protein content. Extraction was performed using a boiling method with distilled water to yield a protein-rich extract, which was then formulated into four suspension formulas (Formula 1, 2, 3, and 4) using pharmaceutical-grade excipients, including sorbitol, sodium

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Martono, Yohanes, Lucia Devi Danriani, and Sri Hartini. "PENGARUH FERMENTASI TERHADAP KANDUNGAN PROTEIN DAN ASAM AMINO PADA TEPUNG GAPLEK YANG DIFORTIFIKASI TEPUNG KEDELAI (Glycine max (L))." Jurnal Agritech 36, no. 01 (2016): 56. http://dx.doi.org/10.22146/agritech.10684.

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Enhancement of proteins and enrichment of amino acids of dried cassava flour can be carried out by fermentation of dried cassava and soy-flour mixture. The purposes of this study were to compare soluble protein's content and to identify amino acids in fortified dried-cassava flour. The methods involved were making fortified dried-cassava flour with ratio of 25 g (soy-flour) and 5 g (yeasts) of 100 grams dried-cassava and fermentation of the mixture for 40 h, measuring soluble protein's content by biuret assay and identifying amino acids using Thin Layer Chromatography (TLC) and High Performanc

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Dwifitri, Uthu, Mohammad Zulkarnain, Rostika Flora, Yetti Purnama, and Samwilson Slamet. "Karakteristik, Asupan Protein, Kadar Protein Total dan Kejadian Kekurangan Energi Kronis pada Ibu Hamil: Studi Cross Sectional." Jurnal Kesehatan Metro Sai Wawai 15, no. 2 (2023): 108–20. http://dx.doi.org/10.26630/jkmsaw.v15i2.3497.

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Latar Belakang: Penanggulangan masalah gizi untuk meningkatkan kualitas sumber daya manusia yang paling baik adalah pada periode kehamilan. Ibu hamil merupakan kelompok yang kritis dan rentan terhadap kekurangan gizi, diantaranya kekurangan energi kronik (KEK). KEK mempunyai dampak buruk terhadap janin pada saat hamil dan sebagian besar tidak dapat dikoreksi setelah bayi lahir. Tujuan: Penelitian ini bertujuan untuk mengevaluasi faktor-faktor yang berhubungan dengan KEK pada ibu hamil. Kajian faktor risiko atau penyebab KEK sebagai rekomendasi intervensi menurunkan kejadian KEK. Metode: Peneli

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Pentury, Melkhianus Hendrik. "Pengaruh Formulasi Tepung Mangrove (Bruguiera gymnorrhiza) dan Tepung Wortel (Daucus carota) terhadap Nilai Gizi dan Organoleptik Nugget Ikan Cakalang (Katsuwonus pelamis)." Agrikan: Jurnal Agribisnis Perikanan 12, no. 2 (2020): 350. http://dx.doi.org/10.29239/j.agrikan.12.2.350-359.

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Formulasi bahan olahan pangan dengan penambahan Sayuran kedalam salah satu produk akan menaikan nilai gizi dan penerimaan produk bagi konsumen. Wortel (Daucus carota L) termasuk kelompok sayuran yang memiliki umbi berwarna jingga, yang kaya akan kandungan gizi yaitu β-karoten sebagai sumber antioksidan alami, serat pangan, tokoferol, asam askorbat, dan α-tokoferol. Tepung Mangrove (Bruguiera gymnorrhiza) adalah sumber pangan lokal yang kandungan karbohidratnya tinggi. Ikan Cakalang (Katsuwonus pelamis) adalah ikan yang berpotensi cukup tinggi serta memiliki nilai ekonomis dan banyak disukai ma

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