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Journal articles on the topic 'Blastocyst hatching process'

Author: Grafiati

Published: 6 September 2023

Last updated: 31 July 2025

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1

Cheon, Yong Pil, Myung Chan Gye, Chung-hoon Kim, et al. "Role of actin filaments in the hatching process of mouse blastocyst." Zygote 7, no. 2 (1999): 123–29. http://dx.doi.org/10.1017/s0967199499000477.

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Hatching has been suggested to occur as a result of protease-mediated lysis and the blastocoele tension. However, even if rupturing is initiated at multiple sites, interestingly only a single site is used for escape. This implies that there are several mechanisms involved in hatching. In this study, the involvement of actin filaments in mouse embryo hatching was examined. We treated mouse embryos with cytochalasin B for 12 h or 24 h at the morula, middle blastocyst, expanded blastocyst, lobe-formed blastocyst and hatching blastocyst stages, and measured the amount and distribution of actin fil

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Kim, Y. J., K. S. Ahn, S. M. Park, B. C. Lee, H. Shim, and C. Ahn. "28 IMPROVED HATCH RATE AFTER PARTIAL DISSECTION OF ZONA PELLUCIDA IN CLONED PIG EMBRYO." Reproduction, Fertility and Development 29, no. 1 (2017): 121. http://dx.doi.org/10.1071/rdv29n1ab28.

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For last 20 years, the efficiency of animal cloning has remained extremely low, despite many attempts to improve it. Although nuclear transfer experiments have been almost optimized, artificial holes are inevitably made in the zona pellucida (ZP) during nuclear transfer experiments, such as enucleation of maternal genome or injection of nuclear donor. Hatching from the ZP is a prerequisite for mammalian embryo implantation, and the condition of the ZP has a lot of influence on hatching. The present studies were performed to investigate the effects of artificial holes in the ZP, because of the

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Eswari, S., G. Sai Kumar та G. Taru Sharma. "Expression of mRNA encoding leukaemia inhibitory factor (LIF) and its receptor (LIFRβ) in buffalo preimplantation embryos produced in vitro: markers of successful embryo implantation". Zygote 21, № 2 (2012): 203–13. http://dx.doi.org/10.1017/s0967199412000172.

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SummaryThe objective of this study was to evaluate the effect of supplementation of recombinant leukaemia inhibitory factor (LIF) in culture media on blastocyst development, total cell number and blastocyst hatching rates and the reverse transcription-polymerase chain reaction analysis of preimplantation buffalo embryos to determine whether they contain the LIF-encoding mRNA and its beta receptor (LIFRβ) genes in different stages of preimplantation buffalo embryos. Cumulus–oocyte complexes retrieved from slaughterhouse buffalo ovaries were matured in vitro and fertilized using frozen buffalo s

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Leonavicius, Karolis, Christophe Royer, Chris Preece, Benjamin Davies, John S. Biggins, and Shankar Srinivas. "Mechanics of mouse blastocyst hatching revealed by a hydrogel-based microdeformation assay." Proceedings of the National Academy of Sciences 115, no. 41 (2018): 10375–80. http://dx.doi.org/10.1073/pnas.1719930115.

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Mammalian embryos are surrounded by an acellular shell, the zona pellucida. Hatching out of the zona is crucial for implantation and continued development of the embryo. Clinically, problems in hatching can contribute to failure in assisted reproductive intervention. Although hatching is fundamentally a mechanical process, due to limitations in methodology most studies focus on its biochemical properties. To understand the role of mechanical forces in hatching, we developed a hydrogel deformation-based method and analytical approach for measuring pressure in cyst-like tissues. Using this appro

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Morató, Roser, Míriam Castillo-Martín, Marc Yeste, and Sergi Bonet. "Cryotolerance of porcine in vitro-produced blastocysts relies on blastocyst stage and length of in vitro culture prior to vitrification." Reproduction, Fertility and Development 28, no. 7 (2016): 886. http://dx.doi.org/10.1071/rd14203.

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The aim of our study was to assess whether the cryotolerance of in vitro-produced embryos could be influenced by the length of in vitro culture and size of blastocoel cavity before vitrification, using the pig as a model. For this purpose we analysed the cryoresistance and apoptosis rate of blastocysts at different stages of development as derived on Day 5 and 6 of in vitro culture. Blastocysts were subsequently vitrified, warmed and cultured for 24 h. Re-expansion rates were recorded at 3 and 24 h and total cell number and apoptotic cells were determined at 24 h. Day-6 blastocysts showed the

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Pasquariello, R., J. R. Herrick, Y. Yuan, et al. "73 Fatty Acid Supplementation in Culture Medium with Reduced Nutrient Concentrations Improves Bovine Blastocyst Development Compared with Standard Culture Medium." Reproduction, Fertility and Development 30, no. 1 (2018): 175. http://dx.doi.org/10.1071/rdv30n1ab73.

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Lipids are a potent source of cellular energy and are metabolized within mitochondria via fatty acid β-oxidation, a process that also requires carnitine. Embryos metabolize lipids during pre-implantation development, but relatively little is known about the effect of fatty acid supplementation for early bovine embryogenesis in culture. The objective of this study was to evaluate the effect of lipid supplementation (via albumin) and l-carnitine (C; 5 mM) during embryo culture in a novel medium with reduced concentrations of nutrients, compared with our standard culture medium (control). Followi

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Fujishima, Ayaka, Kazumasa Takahashi, Mayumi Goto, et al. "Analysis of Na+ concentration patterns in trophectoderm cells of mouse blastocysts using a dual-wavelength electrolyte indicator." PLOS One 20, no. 4 (2025): e0322286. https://doi.org/10.1371/journal.pone.0322286.

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The developmental process of the mammalian blastocyst involves the intricate interplay of cellular and molecular mechanisms, including electrolyte dynamics within the trophectoderm (TE). We hypothesized that sodium (Na+) is actively transported from the TE into the blastocyst cavity, driving water influx and promoting blastocyst expansion. In this study, we investigated the dynamics of Na+ concentration in the TE of mouse embryos using sodium-binding benzofuran isophthalate (SBFI), a dual-wavelength Na+-sensitive fluorescent indicator. Observations revealed three distinct patterns of Na+ dynam

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Ribeiro, E. S., M. C. Gonçalves, M. C. Pedrotti, et al. "74 EFFECT OF BETA-MERCAPTOETHANOL ON THE VITRIFICATION CRYOTOLERANCE OF BOVINE IN VITRO-PRODUCED EMBRYOS." Reproduction, Fertility and Development 21, no. 1 (2009): 137. http://dx.doi.org/10.1071/rdv21n1ab74.

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The control of oxidative processes in in vitro production (IVP) systems by the use of additives may be an alternative approach to improve embryo cryotolerance. The aim of this study was to verify the effect of β-mercaptoethanol (βME) on the cryotolerance of bovine IVP embryos. In 7 replications, and following IVM-IVF, presumptive zygotes (n = 3735) were in vitro-cultured in SOF medium supplemented or not with 100 μm βME (IVC treatment), at 38.5°C and high humidity. The initial 24 h of IVC was performed in 5% CO2 in air, with the remaining 6 days of IVC carried out in 5% CO2, 5% O2, and 90% N2.

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Silva, Thiago Velasco Guimarães, Priscila Di Paula Bessa Santana, Eduardo Baia de Souza, et al. "Sperm chromatin protamination influences embryo development in unsexed and sexed bull semen." Zygote 29, no. 4 (2021): 264–69. http://dx.doi.org/10.1017/s0967199420000775.

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SummarySex selection through sperm sorting offers advantages in regards selection pressure in high-producing livestock. However, the sex-sorting process results in sperm membrane and DNA damage that ultimately decrease fertility. We hypothesized that given the role of protamines in DNA packaging, protamine deficiency could account, at least partially, for the DNA damage observed following sperm sex sorting. To test this, we compared protamine status between unsexed and sexed spermatozoa from two bulls using the fluorochrome chromomycin A3 (CMA3) and flow cytometry. Then, we assessed embryo dev

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Rascado, T. S., M. D. Guastali, D. M. Paschoal, et al. "208 COMPARISON OF THE EXPRESSION OF SOX2 AND Stat3 IN BOVINE BLASTOCYST AND HATCHED BLASTOCYST." Reproduction, Fertility and Development 25, no. 1 (2013): 252. http://dx.doi.org/10.1071/rdv25n1ab208.

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The investigation of the transcription factors involved in the regulation of pluripotency in pre-implantation embryos of cattle provides important information regarding the early embryonic development and derivation of embryonic stem cells. The present experiment aimed to compare the level of expression of SOX 2 and Stat3, pluripotency markers, in bovine blastocysts (D7) and hatched blastocysts (D10), because it is known that around hatching, the inner cell mass (ICM) differentiates into hypoblast and epiblast. Cumulus–oocyte complexes were matured in TCM 199 for 24 h and fertilized with froze

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Das, S. K., X. N. Wang, B. C. Paria, et al. "Heparin-binding EGF-like growth factor gene is induced in the mouse uterus temporally by the blastocyst solely at the site of its apposition: a possible ligand for interaction with blastocyst EGF-receptor in implantation." Development 120, no. 5 (1994): 1071–83. http://dx.doi.org/10.1242/dev.120.5.1071.

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Heparin-binding EGF-like growth factor (HB-EGF) is a newly discovered member of the EGF family of growth factors. HB-EGF can bind to two loci on cell surfaces, heparan sulphate proteoglycans and EGF-receptor (EGF-R), and either one or both of these interactions could play a role in cell-cell interactions. In the rodent, increased endometrial vascular permeability at the site of blastocyst apposition is considered to be an earliest discernible prerequisite event in the process of implantation and this event coincides with the initial attachment reaction between the blastocyst trophectoderm and

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Sidrat, Tabinda, Abdul Aziz Khan, Muhammad Idrees та ін. "Role of Wnt Signaling During In-Vitro Bovine Blastocyst Development and Maturation in Synergism with PPARδ Signaling". Cells 9, № 4 (2020): 923. http://dx.doi.org/10.3390/cells9040923.

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Wnt/β-catenin signaling plays vital role in the regulation of cellular proliferation, migration, stem cells cell renewal and genetic stability. This pathway is crucial during the early developmental process; however, the distinct role of Wnt/β-catenin signaling during pre-implantation period of bovine embryonic development is obscure. Here, we evaluated the critical role of Wnt/β-catenin pathway in the regulation of bovine blastocyst (BL) development and hatching. 6 bromoindurbin-3’oxime (6-Bio) was used to stimulate the Wnt signaling. Treatment with 6-Bio induced the expression of peroxisome

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Ramos-Ibeas, Priscila, Ismael Lamas-Toranzo, Álvaro Martínez-Moro, et al. "Embryonic disc formation following post-hatching bovine embryo development in vitro." Reproduction 160, no. 4 (2020): 579–89. http://dx.doi.org/10.1530/rep-20-0243.

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Failures during conceptus elongation are a major cause of pregnancy losses in ungulates, exerting a relevant economic impact on farming. The developmental events occurring during this period are poorly understood, mainly because this process cannot be recapitulated in vitro. Previous studies have established an in vitro post-hatching development (PHD) system that supports bovine embryo development beyond the blastocyst stage, based on agarose gel tunnels and serum- and glucose-enriched medium. Unfortunately, under this system embryonic disc formation is not achieved and embryos show notorious

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Almiñana, C., E. Corbin, G. Harichaux, et al. "78 INTERCEPTION OF EXOSOMAL MESSAGES BETWEEN THE OVIDUCT AND THE EMBRYO: WHAT ARE THEY TWEETING ABOUT?" Reproduction, Fertility and Development 28, no. 2 (2016): 168. http://dx.doi.org/10.1071/rdv28n2ab78.

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Successful pregnancy requires an appropriate communication between the mother and the embryo(s). Recent studies indicate that exosomes, small (30–100 nm) membrane-bound vesicles of endocytotic origin, could act as intercellular vehicles in this unique communication system in the uterus. However, little is known about the role of these vesicles in the oviduct. Our study aimed at (1) demonstrating the existence of oviducal-embryo communication via exosomes, (2) deciphering the exosomal dialogue between them at the proteomic level, and (3) comparing the exosomal proteomic content to the oviducal

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Forell, F., C. Feltrin, A. D. Vieira, U. M. Costa, M. Hoelker, and J. L. Rodrigues. "52 ESTABLISHMENT OF A PREGNANCY WITH IDENTICAL TWINS AFTER THE TRANSFER OF A VITRIFIED CLONED BOVINE EMBRYO." Reproduction, Fertility and Development 22, no. 1 (2010): 184. http://dx.doi.org/10.1071/rdv22n1ab52.

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Identical twin pregnancies occur naturally in some species because of spontaneous embryo division during initial stages in development. Bovine embryo splitting usually does not compromise subsequent in vivo development, being a method commonly used in embryo transfer programs to enhance overall pregnancy and parity rates. The goal of this study was to establish pregnancies from vitrified bovine cloned embryos. A spontaneous twin pregnancy was obtained after the transfer of a vitrified cloned hatching blastocyst produced by nuclear transfer procedures. Briefly, selected COCs obtained via slicin

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Callesen, H., and P. Holm. "57 DEVELOPMENTAL CHARACTERISTICS OF LATER-STAGE PORCINE EMBRYOS PRODUCED IN VIVO OR IN VITRO." Reproduction, Fertility and Development 28, no. 2 (2016): 158. http://dx.doi.org/10.1071/rdv28n2ab57.

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Kinetics and morphological characteristics during pre-implantation embryo development are well established in most mammals. In porcine, such studies are few because of limited use of in vitro culture, but this has changed in recent years due to increasing use of in vitro production and cloning. Therefore, additional characterisation of especially later stage porcine embryos is needed. Here we studied in vitro development of porcine in vivo- and in vitro-derived zygotes collected from weaned, inseminated sows (slaughtered 2 days after first insemination; in vivo group, n = 112) or produced from

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Amir, Anna Aryani, Jennifer M. Kelly, David O. Kleemann, Zoey Durmic, Dominique Blache, and Graeme B. Martin. "Extracts of forage plants affect the developmental competence of ovine oocytes in vitro." Animal Production Science 59, no. 10 (2019): 1814. http://dx.doi.org/10.1071/an18170.

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Forage plants may contain secondary compounds that disrupt reproduction in ruminants so, as ‘duty of care’, proposed new forage species need to be tested for harmful effects on reproduction before industrial release. We evaluated the effects of Bituminaria bituminosa, Medicago sativa, Chicorium intybus, Trifolium subterraneum, Trifolium pratense, Biserrula pelecinus and Eremophila glabra, on the in vitro developmental competence of ovine oocytes. Crude methanolic extracts of each plant were added to the medium (final concentrations: 0, 50 or 100 μg dry extract per mL) used for in vitro maturat

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Nims, S., D. Melican, T. Jellerette, R. Butler, and W. Gavin. "114EFFECT OF CYTOCHALASIN B TREATMENT ON VITRIFICATION OF CAPRINE PARTHENOGENIC BLASTOCYSTS." Reproduction, Fertility and Development 16, no. 2 (2004): 179. http://dx.doi.org/10.1071/rdv16n1ab114.

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Production of human recombinant proteins in the milk of transgenic animals has been shown to be a viable production system. Protection of the animal genetics involved is paramount. Vitrification of embryos is a simple, time-efficient way of preserving an animal’s genetics without the formation of damaging ice crystals during the freezing process. Cytochalasin B has been shown to increase the viability of porcine blastocysts by reducing damage to microfilaments and other cytoskeletal components. These experiments utilized caprine parthenogenic blastocysts as a model to compare the viability of

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Kuzmany, A., V. Havlicek, C. Wrenzycki, S. Wilkening, G. Brem, and U. Besenfelder. "76 EFFECT OF CULTURE METHOD ON THE mRNA EXPRESSION BEFORE AND AFTER CRYOPRESERVATION IN BOVINE BLASTOCYSTS." Reproduction, Fertility and Development 23, no. 1 (2011): 143. http://dx.doi.org/10.1071/rdv23n1ab76.

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Blastocyst mRNA expression and cryopreservability are thought to be suitable indicators of embryo quality and developmental competence and have been shown to be affected by production methods and culture systems. The aim of the present study was to assess cryosurvival and levels of mRNA expression of selected genes [occludin, desmocollin 2, solute carrier family 2 member 3 (formerly glucose transporter 3), BAX, BCL xL, heat shock protein A1A (formerly heat shock protein 70.1), aquaporin 3, and DNA methyltransferase 1a] of bovine blastocysts derived by 4 different, established culture methods [

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Popelková, M., P. Chrenek, J. Pivko, A. V. Makarevich, E. Kubovičová, and J. Kačmárik. "Survival and ultrastructure of gene-microinjected rabbit embryos after vitrification." Zygote 13, no. 4 (2005): 283–93. http://dx.doi.org/10.1017/s096719940500331x.

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Morphological signs of injury and subsequent regeneration following vitrification of either rabbit gene microinjected (Gene-Mi) or intact in vitro cultured embryos derived from in vivo fertilized eggs were evaluated by post-warming recovery in culture and analysed by transmission electron microscopy (TEM). The percentages of vitrified/warmed Gene-Mi embryos that reached the blastocyst stage (69%) and hatched (57%) did not differ significantly from those of intact embryos (78% and 56%, respectively). In contrast, in vitro development of embryos to the blastocyst stage among non-vitrified intact

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Campos-Chillon, L., T. Suh, E. Carnevale, and G. Seidel. "241 EFFECT OF MEIOTIC ARREST BY ROSCOVITINE AND SUBSEQUENT IVM TIME ON DEVELOPMENTAL COMPETENCE OF IMMATURE BOVINE OOCYTES." Reproduction, Fertility and Development 17, no. 2 (2005): 271. http://dx.doi.org/10.1071/rdv17n2ab241.

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Maintaining immature bovine oocytes at the germinal vesicle stage by inhibiting M-phase promoting factor (MPF) activity is a reversible process when using roscovitine, and this can improve cytoplasmic maturation in vitro. However, optimum meiotic arrest times and subsequent IVM times have not been determined, so we evaluated the developmental competence of oocytes in relation to these times. Two by two factorial treatments consisting of 2 arrest times (8 h, 16 h) and 2 subsequent IVM times (16 h, 22 h) plus a control were replicated 6 times in this study. Semen from two bulls was used three ti

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Malhotra, Keshav. "The Different Biopsy Techniques." Fertility & Reproduction 05, no. 04 (2023): 214. http://dx.doi.org/10.1142/s2661318223740286.

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Preimplantation genetic testing needs access to genomic DNA in order to execute a genetic test on a preimplantation embryo. Normally a few cells must be extracted or ‘biopsied’ from the embryo to achieve this. Embryo biopsy evolved from attempts in the 1960s and early 1970s to sex farm animal embryos to now utilizing it to screen and select human embryos based on Ploidy, or avoid monogenic disorders. In October 1989, the first clinical cleavage stage biopsies and single cell PGT to determine the sex of the embryos were conducted. It has now evolved to utilize Trophectoderm cells. The advent of

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Domingo-Muelas, Ana, Robin M. Skory, Adam A. Moverley, et al. "Human Embryo Live Imaging Reveals Nuclear DNA Shedding During Blastocyst Expansion and Biopsy." Obstetrical & Gynecological Survey 78, no. 10 (2023): 587–88. http://dx.doi.org/10.1097/01.ogx.0000993700.13780.f9.

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ABSTRACT The proportion of babies born from in vitro fertilization (IVF) is rising at an exponential rate, highlighting the importance that we have a comprehensive understanding of human preimplantation development and determinants of embryo quality. The early divisions of the embryo after fertilization but before implantation and resulting mitotic errors have been studied primarily in the mouse embryo; however, thus far we have lacked the technology to characterize these critical steps in humans. Establishing an approach that can bypass genetic manipulation and microinjections of DNA or mRNA

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Fanti, T., N. M. Ortega, R. Garaguso, M. J. Franco, C. Herrera, and A. A. Mutto. "239 USE OF CYCLIC ADENOSINE MONOPHOSPHATE MODULATORS IN IN VITRO PRODUCTION OF BOVINE EMBRYOS." Reproduction, Fertility and Development 27, no. 1 (2015): 209. http://dx.doi.org/10.1071/rdv27n1ab239.

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In vitro embryo production systems (IVP) try to emulate and enhance molecular events that occur in in vivo reproductive systems in order to increase, not only the number of embryos generated, but also their quality. Despite advances, IVP processes are still inefficient compared with in vivo systems. Several studies have attributed this deficiency to a lack of oocyte competence due to spontaneous premature resumption of meiotic maturation in the oocyte following the removal from its follicular environment. Therefore, our objective was to increase oocyte competence avoiding premature resumption

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Zhang, Yan, Jing Guo, Xiao Wei Nie, et al. "Rosmarinic acid treatment during porcine oocyte maturation attenuates oxidative stress and improves subsequent embryo development in vitro." PeerJ 7 (June 18, 2019): e6930. http://dx.doi.org/10.7717/peerj.6930.

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Background In vitro maturation (IVM) of oocytes has been widely used in the field of assisted reproductive technology. However, oocytes can be injured by oxidative stress during the process of IVM. Methods The present study was designed to evaluate the influences of rosmarinic acid (RA) on the IVM of porcine oocytes and the subsequent development of early-stage embryos as well as its underlying mechanisms. Various concentrations of RA (5 µM, 10 µM, and 25 µM) were treated with porcine oocyte maturation medium during the period of IVM. Results and Discussion The results showed that 5 µM RA trea

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Nguyen, Khanh Van, Thi Kim Yen Pham, Thi Au Hoang, Quang Minh Luu, and Doan Lan Pham. "Influence of fetal calf serum on the production of bovine embryos in vitro." Ministry of Science and Technology, Vietnam 65, no. 1 (2023): 76–80. http://dx.doi.org/10.31276/vjste.65(1).76-80.

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The aim of the present study was to evaluate the effect of foetal calf serum (FCS) on the production of bovine embryo in vitro. We conducted two experiments: (1) to evaluate the effect of FCS on the production of bovine embryo in vitroand (2) to evaluate the effect of duration of FCS supplementation on the production of bovine embryo in vitro. In experiment 1, we divided mature bovine oocytes after in vitro fertilization into four embryo culture media: CR1aa, CR1aa+FCS, SOFaa, and SOFaa+FCS. Embryos cultured in CR1aa and SOFaa media supplemented with FCS resulted in a higher cleavage and blast

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García-Jiménez, Maria, Klaus Rink, Enric Mestres, Ivette Vanrell, Gloria Calderón, and Nuno Costa-Borges. "Evaluation of the impact of laser-assisted hatching techniques on the hatching process of mouse blastocysts using time-lapse microscopy." F&S Science 2, no. 1 (2021): 43–49. http://dx.doi.org/10.1016/j.xfss.2020.12.004.

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Yamazaki, Kimie, Rika Suzuki, Eriko Hojo, et al. "Trypsin-like Hatching Enzyme of Mouse Blastocysts: Evidence for Its Participation in Hatching Process before Zona Shedding of Embryos6. (embryo/hatching enzyme/protease/trypsin/strypsin)." Development, Growth and Differentiation 36, no. 2 (1994): 149–54. http://dx.doi.org/10.1111/j.1440-169x.1994.00149.x.

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McMillan, W. H., A. J. Peterson, S. F. Cox, S. J. Pearson, and M. J. Donnison. "Reproductive performance during early pregnancy in cattle with contrasting early pregnancy rates." BSAP Occasional Publication 26, no. 2 (2001): 283–88. http://dx.doi.org/10.1017/s0263967x00033796.

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AbstractCauses of variation amongst cattle within a herd in their ability to initiate and maintain pregnancy are largely unknown. An experimental animal resource has recently been established to understand the biology of early reproductive performance. This paper summarises the results achieved during the establishment phase and from several experiments aimed at determining the physiological basis of the difference between sub-herds of contrasting pregnancy rates on Day 60. Each of 155 contemporary yearling heifers received 2 in vitro-produced embryos on 6 separate occasions during a 26-month

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Rogers, K. D., B. A. Foster, E. J. Guiterrez, F. A. Diaz, and K. R. Bondioli. "41 Effects of Dimethyl Sulfoxide- or Glycerol-Based Vitrification Protocols on Zona Pellucida Hardening in Mature Bovine Oocytes." Reproduction, Fertility and Development 30, no. 1 (2018): 160. http://dx.doi.org/10.1071/rdv30n1ab41.

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Zona pellucida hardening is a natural process that occurs after oocyte fertilization to prevent polyspermic fertilization and to protect embryonic development. Pre-fertilization hardening of the zona pellucida, however, decreases fertilization rates. Cryoprotectants have also been shown to negatively affect fertilization rates, one possible mechanism of which being through zona hardening. This experiment was conducted to determine the effect of different cryoprotectants on hardening of the zona pellucida of mature bovine oocytes. Oocytes were collected by ovum pick-up (OPU) by transvaginal ult

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Stinshoff, H., K. Brüning, A. Hanstedt, D. Müller, S. Wilkening, and C. Wrenzycki. "117 EFFECT OF DIFFERENT CRYOPRESERVATION METHODS ON THE QUALITY OF IN VITRO-PRODUCED BOVINE EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 217. http://dx.doi.org/10.1071/rdv22n1ab117.

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In vitro production (IVP) of bovine embryos has been greatly improved over the last couple of years. However, only one-third of the total number of embryos transferred worldwide are of in vitro origin. The IVP embryos still show remarkable differences compared with their in vivo-derived counterparts (i.e. bovine embryos produced in vitro are more sensitive to cryopreservation). So far, vitrification seems to be the most promising method to cryopreserve in vitro-produced bovine embryos. The aim of this study was to determine the effect of 2 different cryopreservation methods on the quality of i

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Oliveira, Clara Slade, Naiara Zoccal Saraiva, Maria Helena Coelho Cruz, et al. "HDAC inhibition decreases XIST expression on female IVP bovine blastocysts." REPRODUCTION 145, no. 1 (2013): 9–17. http://dx.doi.org/10.1530/rep-11-0343.

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During initial development, both X chromosomes are active in females, and one of them must be silenced at the appropriate time in order to dosage compensate their gene expression levels to male counterparts. Silencing involves epigenetic mechanisms, including histone deacetylation. Major X chromosome inactivation (XCI) in bovine occurs between hatching and implantation, althoughin vitroculture conditions might disrupt the silencing process, increasing or decreasing X-linked gene expression. In this study, we aimed to address the roles of histone deacetylase inhibition by trichostatin A (TSA) o

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Sidrat, Tabinda, Abdul Aziz Khan, Myeong-Don Joo, et al. "Extracellular vesicles improve embryo cryotolerance by maintaining the tight junction integrity during blastocoel re-expansion." Reproduction 163, no. 4 (2022): 219–32. http://dx.doi.org/10.1530/rep-21-0320.

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Cryopreservation is a process in which the intact living cells, tissues, or embryos are preserved at subzero temperatures for preservation. The cryopreservation process highly impacts the survival and quality of the in vitro-produced (IVP) embryos. Some studies have highlighted the use of oviduct extracellular vesicles (EVs) to improve the cryotolerance of IVP embryos but the mechanism has not been well studied. The present study unravels the role of in vitro cultured bovine oviduct epithelial cells-derived EVs in improving the re-expansion and hatching potential of thawed blastocysts (BLs). T

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Herrera, E. Y., C. de Frutos, R. Laguna-Barraza, A. Gutierrez-Adan, and D. Rizos. "88 EFFECT OF VITRIFICATION PROCEDURE ON SURVIVAL RATE OF BOVINE EMBRYOS PRODUCED IN VITRO." Reproduction, Fertility and Development 23, no. 1 (2011): 149. http://dx.doi.org/10.1071/rdv23n1ab88.

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Vitrification as a cryopreservation method has many advantages compared with slow freezing. Many variables in the vitrification process exists that influence the survival rates of vitrified oocytes and embryos. These include the cryoprotectants (type, concentration, and duration of exposure), the temperature of the vitrification solution at exposure, the device used for vitrification, and the quality and developmental stage of embryos. It is worthwhile to mention that vitrification protocols successfully used in bovine oocytes and embryos have been used also with human oocytes and embryos. Vit

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Luz, M. R., C. C. Holanda, J. J. Pereira, et al. "99 SURVIVAL RATE AND IN VITRO DEVELOPMENT OF IN VIVO-PRODUCED AND CRYOPRESERVED DOG EMBRYOS." Reproduction, Fertility and Development 22, no. 1 (2010): 208. http://dx.doi.org/10.1071/rdv22n1ab99.

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Dogs have been used as an experimental model for human genetic diseases and for research applied to endangered Canidae. Moreover, application of assisted reproductive technologies (ART) by dog breeders is increasing. The aim of this study was to evaluate the survival rate and in vitro development of in vivo-produced and cryopreserved dog embryos. Seven cross-bred bitches were submitted to ovariohysterectomy 12 days after first mating or artificial insemination, and embryos were recovered by uterine horn flushing with 30 mL of PBS/horn (Nutricell, Campinas, SP, Brazil). Grade 1 and 2 morulae (M

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Pathak, Madhulika, Vani Venkatappa, Surendra Sharma та Polani B. Seshagiri. "Expression of IL-1β and implantation serine proteases is required for mouse blastocyst hatching". Reproduction, листопад 2020. http://dx.doi.org/10.1530/rep-20-0376.

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Mammalian blastocyst hatching is critically an indispensable process for successful implantation. One of the major challenges in IVF clinics is to achieve superior embryonic development with intrinsically potent hatching-competent blastocyst. However, the molecular regulation of hatching phenomenon is poorly understood. In this study, we examined the expression and function of one of the cytokines, IL-1β during blastocyst hatching in the mouse. In particular, the expression of IL-1β (Interleukin-1β), IL-1ra (Interleukin-1 receptor antagonist) and their functional receptor IL-1rt1 (Interleukin

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Yang, Guangping, Jianhua Chen, Yanni He та ін. "Neddylation Inhibition Causes Impaired Mouse Embryo Quality and Blastocyst Hatching Failure Through Elevated Oxidative Stress and Reduced IL-1β". Frontiers in Immunology 13 (4 липня 2022). http://dx.doi.org/10.3389/fimmu.2022.925702.

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Mammalian blastocyst hatching is an essential prerequisite for successful embryo implantation. As the rate-limiting step of current assisted reproductive technology, understanding the key factors regulating blastocyst hatching would be significantly helpful to improve the performance of the assisted reproductive practice. In early embryo development, the fine-tuned elimination of maternal materials and the balanced protein turnover are inevitable for the competent to hatch and implant into endometrium. Neddylation, a ubiquitination-like protein modification, has been shown to be involved in oo

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Liu, Y., C. Jones, and K. Coward. "P–182 The mechanism of mouse embryo hatching and the impact of laser drilling the zona pellucida: an RNA sequencing study." Human Reproduction 36, Supplement_1 (2021). http://dx.doi.org/10.1093/humrep/deab130.181.

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Abstract Study question What is the mechanism of embryo hatching? Will laser-assisted zona pellucida (ZP) drilling alter the embryonic transcriptome? Summary answer Hatching is an ATP-dependent process. Hatching is also associated with Rho-mediated signaling. Laser-assisted ZP drilling might cause alternation in embryo metabolism. What is known already Embryo hatching is a vital process for early embryo development and implantation. Animal data suggests that hatching is the result of multiple factors, such as mechanical pressure, protease activation, and the regulation of maternal secretions.

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Pravdyuk, O., M. Gryshchenko, L. Shatalova, and K. Borodai. "P–163 The presence of trophectodermal vesicles on expanded ICSI-derived blastocysts is a good predictor of implantation." Human Reproduction 36, Supplement_1 (2021). http://dx.doi.org/10.1093/humrep/deab130.162.

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Abstract Study question Is the implantation rate (IR) higher in blastocysts with trophectodermal vesicles (TVs) compared to blastocysts without TVs or euploid blastocysts with unknown spontaneous hatching status? Summary answer The blastocysts with TVs demonstrate significantly higher IR in comparison to blastocysts without TVs or euploid blastocysts with unknown spontaneous hatching status. What is known already After ICSI spontaneous hatching mainly occurs by trophectoderm cell herniation via a small slit in the zona pellucida. At the beginning of this process, TVs are formed on the outside

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Adams, T., M. Markle, and C. Leisinger. "P-527 Comparison of implantation rate and preimplantation genetic testing between hatching and hatched blastocysts." Human Reproduction 37, Supplement_1 (2022). http://dx.doi.org/10.1093/humrep/deac107.486.

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Abstract Study question Does embryo stage, hatching versus hatched blastocyst, affect ploidy status and implantation rates (IR)? Summary answer The embryo stage may influence ploidy status, but no correlation was made between embryo stage and IR. What is known already It is generally accepted that hatched embryos present increased vulnerability when handled and are often selected against. The literature on this varies with some reporting that the transfer of hatched blastocysts resulted in lower pregnancy rates compared to hatching or expanded blastocysts. Other studies have found no differenc

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Bayat, H., M. Akbari, S. Adib, and B. Movaghar. "P-113 Comparison of mitochondrial function between expanded and hatching human blastocysts." Human Reproduction 39, Supplement_1 (2024). http://dx.doi.org/10.1093/humrep/deae108.487.

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Abstract Study question Is there a difference in mitochondrial function between expanded and hatching fresh and vitrified blastocysts? Summary answer Freezing and thawing may lead to mitochondrial dysfunction. What is known already Mitochondria, as crucial cellular organelles, play role in energy production through the electron transport chain, cell motility and organelle movement. They also have a significant impact on embryonic development. Furthermore, during the process of embryonic hatching, the energy generated by these organelles can affect ion pump activity, blastocyst expansion, and c

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Herrero Grassa, L., J. Ten Morro, Á. Linares Bernabéu, et al. "P-178 Time to hatching: the most predictive morphokinetic parameter for embryo implantation in machine learning algorithms following time-lapse incubation and blastocyst transfer in oocyte-donation programs." Human Reproduction 37, Supplement_1 (2022). http://dx.doi.org/10.1093/humrep/deac107.172.

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Abstract Study question Which is the most predictive parameter when machine learning (ML) is applied to a known implantation database (KID) of day 5 embryo transfer database in an egg donation program? Summary answer Time to hatching (tiHB) is the most predictive embryonic parameter when machine learning algorithms were used on reproductive data in an oocyte donation program. What is known already Artificial intelligence is becoming an encouraging tool in medicine, also in ART, where the amount of data generated in the IVF lab has dramatically increase, favored by time-lapse technology. Numero

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Sanguinet, P., G. Regnier-Vigouroux, B. Keppi, et al. "P-286 Can laser assisted hatching help implantation of warmed blastocysts in presence of fragmented cells ?" Human Reproduction 37, Supplement_1 (2022). http://dx.doi.org/10.1093/humrep/deac107.275.

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Abstract Study question Does laser assisted hatching (LAH) following warming of blastocysts containing fragmentated cells improves blastocyst expansion and implantation? Summary answer The application of LAH does not improve blastocyst expansion and implantation regardless the presence or absence of fragmented cells. What is known already The absence of natural hatching is one of the hypotheses of implantation failure after cryopreservation, a process that could lead to hardening of the zona pellucida. Another theory is the fact that cellular fragments inside the zona pellucida, surrounding th

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Zhang, Ling, Yi-er Zhou, Yue-jin Wu, et al. "Thinning or Opening: A Randomized Sibling-Embryo Pilot Trial on the Efficacy of Two Laser-Assisted Hatching Modes During the Extended Culture of Highly Fragmented Cleavage Embryos." Frontiers in Endocrinology 13 (June 27, 2022). http://dx.doi.org/10.3389/fendo.2022.927834.

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A randomized sibling-embryo pilot trial investigated whether two ways of laser-assisted hatching result in different blastulation and clinical outcomes after extended in vitro culture process of highly fragmented day-3 cleavage embryos. From 92 couples, a total of 315 highly fragmented day-3 embryos (the fragmentation &gt;25%) were recruited and randomized into laser-assisted zona thinning (LAT, n=157) and opening (LAO, n=158) groups, and then underwent a blastocyst culture in vitro. The main endpoint measurements including blastocyst formation and grading as well as the clinical pregnancy

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Son, W. Y., A. M. N. M. Atared, H. S. Abukhaled, D. H. Al Ratrout, S. Namiq, and A. M. Shahrani. "P-209 One-step warming of vitrified human blastocyst-stage embryos: impact of sucrose or trehalose concentrations on survival and developmental potential." Human Reproduction 40, Supplement_1 (2025). https://doi.org/10.1093/humrep/deaf097.518.

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Abstract Study question Does one-step warming (OW), a simplified embryo warming protocol using varying sucrose or trehalose (S/T) concentrations, affect the survival and developmental potential of vitrified blastocysts? Summary answer OW using different concentrations of S/T showed no significant differences in the survival or developmental potential of vitrified blastocyst-stage embryos. What is known already Standard embryo warming protocols typically involve a multi-step process with stepwise osmotic solutions (1.0M S/T) to prevent rapid water influx into embryos. Recent studies suggest OW

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Wang, Xiaoxia, Jing Zhao, Zhongyuan Yao, et al. "Arrested Cells/Cellular Debris Expelled from Blastocysts Is Self-Correction Phenomenon During Early Embryonic Development." Reproductive Sciences, January 10, 2023. http://dx.doi.org/10.1007/s43032-022-01159-8.

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Abstract Arrested cells/ cellular debris is component left in the zona pellucida after blastocyst hatching. To identify whether expelling arrested cells/cellular debris from blastocysts is a process of human embryo self-correction by eliminating abnormal cells, 21 pairs of trophectoderm (TE) biopsies and the corresponding arrested cells/cellular debris expelled from the blastocysts from July to December 2020 were collected and analyzed using next-generation sequencing (NGS). Then, the NGS results of TE biopsies and the corresponding arrested cells/cellular debris were compared. We identified t

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Jiang, V., C. Bormann, I. Souter, et al. "P-294 Use of Artificial Intelligence to Assess the Effects of Assisted Hatching on Embryo Development and Implantation Potential." Human Reproduction 37, Supplement_1 (2022). http://dx.doi.org/10.1093/humrep/deac107.282.

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Abstract Study question Does the use of laser-assisted hatching (AH) on cleavage stage embryos affect in vitro preimplantation embryo development or implantation potential? Summary answer There is no difference in blastocyst conversion rate or implantation potential of embryos following AH at the cleavage stage for patients under age 35 years. What is known already Laser-AH is the process of creating an opening within the zona pellucida on cleavage stage embryos to facilitate biopsy of trophectoderm cells for preimplantation genetic testing (PGT). Studies have shown that PGT for aneuploidy (PG

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Watson, K., K. Ong, I. Korman, et al. "O-213 Slow day 5 development affects implantation potential of fresh transferred embryos but not birthweight once pregnancy occurs: A multi-center retrospective cohort study." Human Reproduction 36, Supplement_1 (2021). http://dx.doi.org/10.1093/humrep/deab128.024.

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Abstract Study question Does slow development of fresh transferred day 5 embryos lead to decreased implantation potential and birthweight? Summary answer Slow day 5 development was associated with reduced implantation potential when transferred fresh but the subsequent birthweight of the resulting baby was not impacted. What is known already Slow development of in vitro cultured cleavage stage embryos is associated with reduced blastocyst development and implantation rates. There is no current consensus regarding whether to transfer fresh slow developing day 5 embryos or to extend culture for

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Belchin, P., Y. Cabello, M. Sanche. d. Burgos, et al. "P–158 Assisted Hatching on D + 3 in order to facilitate trophectoderm biopsy in blastocyst for PGT-A is not advisable in all patients." Human Reproduction 36, Supplement_1 (2021). http://dx.doi.org/10.1093/humrep/deab130.157.

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Abstract Study question Is it useful or beneficial to perform Assisted Hatching (AH) on D + 3 previously to biopsy for PGT-A on blastocyst stage on D + 5? Summary answer The routine use of AH on D + 3 to facilitate the embryo biopsy on D + 5 could negatively influence the development of the embryos to blastocyst stage. What is known already The blastocyst stage is the optimal stage for performing biopsies for PGT-A, which has been reported as a key factor determining the growing clinical application of this strategy worldwide. For trophectoderm (TE) biopsy, laser-assisted drilling is used to c

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Cimadomo, D., A. Marconetto, F. Innocenti, et al. "O-101 Elucidation of blastocyst collapse and its consequences: a comprehensive artificial intelligence-powered analysis of 1943 embryos from 643 couples." Human Reproduction 37, Supplement_1 (2022). http://dx.doi.org/10.1093/humrep/deac105.124.

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Abstract Study question What are the causes and consequences of blastocyst collapse? Summary answer ∼50% of blastocysts collapsed, especially if they are aneuploid and/or morphologically-poor. Yet, no impact on the live-birth-rate (LBR) per vitrified-warmed euploid single-embryo-transfer (SET) was reported. What is known already Time-lapse-microscopy (TLM) is a powerful tool to describe the peculiar dynamics of preimplantation development. Lately, artificial intelligence (AI) has been also implemented to automatize and standardize such description. Here, we adopted AI to comprehensively portra

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