Relevant bibliographies by topics / Blood – Collection and preservation

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Academic literature on the topic 'Blood – Collection and preservation'

Author: Grafiati
Published: 4 June 2021
Last updated: 30 July 2024

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Journal articles on the topic "Blood – Collection and preservation"

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Ballen, Karen K., Juliet N. Barker, Susan K. Stewart, Michael F. Greene, and Thomas A. Lane. "Collection and Preservation of Cord Blood for Personal Use." Biology of Blood and Marrow Transplantation 14, no. 3 (March 2008): 356–63. http://dx.doi.org/10.1016/j.bbmt.2007.11.005.

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Walter, Dagmar, Jawad Abousoud, Tingsheng Drennon, Connor Kunihiro, Kelly Martin, Yina Li, Nandhini Raman, Veronica Rodriguez, and Jill Lynden Herschleb. "Blood Collection Workflow for Interrogation of Translational Samples with Single Cell Technologies." Blood 142, Supplement 1 (November 28, 2023): 7182. http://dx.doi.org/10.1182/blood-2023-190485.

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Blood is a valuable and easily accessible sample type for clinical researchers that provides a snapshot of the organ systems at the time of sample collection. Readouts obtained from blood help inform clinical treatments, investigate disease mechanisms, evaluate drug response, and monitor outcomes. However, the impact of existing blood collection workflows on data output from new and high resolution technologies, like single cell RNA sequencing (scRNA-seq) remains a challenge. Recent scRNA-seq studies have shown that changes in gene expression can occur in blood cells in as little as a few hours post collection1. While bulk measurements rely on sample fixation to preserve the sample during transport, this approach does not preserve intact cells and hence, is not compatible with single cell technologies. With the recent availability of fixation compatible scRNA-seq assays comes the need to preserve blood while retaining single cell information. In this study, we evaluated various PBMC isolation and blood preservation methods which preserve single cell information while easing sample logistical constraints. Our initial experiments compared various PBMC isolation methods. scRNA seq analysis of these samples demonstrated that all isolation methods captured single cell gene expression with some variability in cell types along with user-variability. Changes in cell type proportions were observed in as little as 4 hrs post blood draw, emphasizing the need for an effective blood preservation method to retain single cells during transport and blood cell isolation. Next, we compared different methods of blood preservation including cryopreservation and fixation. Healthy and diseased whole blood samples were collected, preserved, stored followed by isolation of blood samples at different time points. scRNA-seq analysis of the samples demonstrated that blood can be preserved, transported, and stored (weeks to months) before blood cell isolation. Blood cells isolated from these cells can then be stored long term for scRNA-seq analysis. This blood collection and preparation workflow also eases blood transportation logistical constraints, allowing for batch shipping of samples from distributed collection sites. Coupled with automatable cell isolation methods and multiplexing single cell sequencing solutions, these workflows can be adapted for large scale translational research studies. References: Massoni-Badosa, R., Iacono, G., Moutinho, C. et al. Sampling time-dependent artifacts in single-cell genomics studies. Genome Biol 21, 112 (2020). Keywords: single cell RNA-seq, blood preservation
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Wilt, Kristen, Charis Ober, and John Garcia. "Abstract 15 A State-Run Public Cord Blood Collection Program." Stem Cells Translational Medicine 12, Supplement_1 (September 1, 2023): S17. http://dx.doi.org/10.1093/stcltm/szad047.016.

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Abstract Introduction The Arizona Public Cord Blood Program was created in 2011 by the Arizona Biomedical Research Centre, a sub-section of the Arizona Department of Health Services, to advance the collection and increase the number of racially and ethnically diverse cord blood units available for transplantation, as well as promote awareness of the benefits of cord blood stem cells through our educational partner, Save the Cord Foundation (STCF). Objectives Create a sustainable program for women of all racial and ethnic backgrounds to have the opportunity to donate cord blood, with the primary goal of transplantation, and secondary goal of providing non-transplantable cord blood units for research. A second objective was to educate the residents of the state of Arizona about cord blood stem cells and the need for their preservation. Methods A portion of state lottery funds supports the program monetarily. Those funds are provided to four collection hospitals who employ "cord blood consenters" whose responsibility it is to consent patients, assist delivery providers with collections, and package and ship cord blood units to our partner cord blood bank at MD Anderson Cancer Center. There are also two clinical coordinators who educate and train hospital staff on quality collection practices, with special emphasis on the importance of high volume, sterile collections. STCF provides education across the state to expectant parents, healthcare providers, schools, and the public about the need for cord blood stem cell donation for transplant and research. Results Since 2011, the Arizona Public Cord Blood Program has banked hundreds of racially and ethnically diverse cord blood units with the National Marrow Donor Program, and has had 86 life-saving cord blood units matched with patients in need around the globe. This innovative program has not only expanded cord blood awareness and promoted the preservation of cord blood; it has resulted in the creation of an economic engine for the state of Arizona that is an attractant for STEM based businesses and careers. Discussion A decade later, the Arizona Public Cord Blood Program has proven to be a sustainable model for collecting and providing suitable cord blood units for transplant to diverse patient populations.
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Spratt, John R., Lars M. Mattison, Paul A. Iaizzo, and Gabriel Loor. "The ABCs of autologous blood collection for ex vivo organ preservation." Journal of Thoracic and Cardiovascular Surgery 155, no. 1 (January 2018): 433–35. http://dx.doi.org/10.1016/j.jtcvs.2017.08.036.

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Wilt, Kristen, Charis Ober, John Garcia, and Jennifer Botsford. "Abstract 33 A Diverse and Sustainable State-Run Public Cord Blood Program." Stem Cells Translational Medicine 11, Supplement_1 (September 1, 2022): S39. http://dx.doi.org/10.1093/stcltm/szac057.033.

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Abstract Introduction The Arizona Public Cord Blood Program was created in 2011 by the Arizona Biomedical Research Centre, a subsection of the Arizona Department of Health Services, to advance the collection and increase the number of racially and ethnically diverse cord blood units available for transplantation, as well as to promote awareness of the benefits of cord blood stem cells through our educational partner, Save the Cord Foundation (STCF). Objective The main objective of this study was to create a sustainable program for women of all racial and ethnic backgrounds to have the opportunity to donate cord blood, with the primary goal of transplantation and the secondary goal of providing non-transplantable cord blood units for research. A second objective was to educate the residents of the state of Arizona about cord blood stem cells and the need for their preservation. Methods A portion of state lottery funds support the program monetarily. Those funds are provided to four partner collection hospitals employing "cord blood consenters," whose responsibility it is to consent patients, assist delivery providers with collections, and package and ship cord blood units to our partner cord blood bank at MD Anderson Cancer Center. There are also two clinical coordinators who educate and train hospital staff on quality collection practices, with special emphasis on the importance of high-volume, sterile collections. STCF provides education across the state to expectant parents, health care providers, schools, and the public about the need for cord blood stem cell donation for transplant and research. Results Since 2011, the Arizona Public Cord Blood Program has banked several hundreds of racially and ethnically diverse cord blood units with the National Marrow Donor Program (Figure 1) and has had 80 life-saving cord blood units matched with patients in need around the globe. This innovative program has expanded cord blood awareness and promoted the preservation of cord blood, and it also has resulted in the creation of an economic engine for the state of Arizona that is an attractant for STEM-based businesses and careers. Discussion A decade later, the Arizona Public Cord Blood Program has proven to be a sustainable model for collecting and providing suitable cord blood units for transplant to diverse patient populations.
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Okada, K., and J. Yasuda. "Methods for Collection and Preservation of Cattle Blood for Metabolic Profile Test." Japanese Journal of Veterinary Clinics 24, no. 1 (2001): 13–18. http://dx.doi.org/10.4190/jjvc2001.24.13.

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Martin, Kelly, Connor Kunihiro, Jawad Abousoud, Tingsheng Drennon, Yina Li, Sean Marrache, Nandhini Raman, et al. "Abstract 995: Preservation of patient whole blood preserves cancer immune cell biology at single cell resolution." Cancer Research 84, no. 6_Supplement (March 22, 2024): 995. http://dx.doi.org/10.1158/1538-7445.am2024-995.

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Abstract Blood is an easily accessible clinical sample type that helps inform treatments, investigate disease mechanisms, and monitor outcomes. Logistical challenges in the transportation and processing of blood samples impact data output from single cell RNA sequencing (scRNA-seq). Recent scRNA-seq studies have shown that significant changes in gene expression and immune cell populations occur in as little as a few hours post collection. Thus, there is a need for rapid preservation of blood upon collection before biological changes can occur. Existing preservation approaches compromise the cell membrane, making samples incompatible with single cell transcriptomic analyses. Here, we describe a novel workflow for fixation and long-term storage of whole blood, and isolation of peripheral blood mononuclear cells (PBMCs) from the fixed blood using a readily available, low-cost, high-throughput procedure. We then profile the gene expression of these cells using the fixation-compatible Single Cell Gene Expression Flex workflow. Importantly, this novel blood collection and preparation workflow eases blood transportation logistical constraints, allowing distributed sample collection and batched shipping of blood samples for scRNA-seq and potentially enabling large-scale translational research studies. We compared whole blood fixed immediately after collection using our workflow with clinical blood samples stored at ambient temperature. This revealed that immune cell populations and biological pathways shift significantly as early as five hours following sample collection, if not immediately stabilized. Across a time course of ambient temperature storage of whole blood without immediate stabilization, we observe significant upregulation of stress-related genes (FOS, JUN, CIRBP) in samples using Single Cell Gene Expression Flex profiling. Additionally, expression of a proto-oncogene TNFAIP3 was upregulated in whole blood stored at 20°C compared to samples stored at 4°C for the same lengths of time, unless samples were stabilized using the novel workflow shown here. This novel whole blood fixation method, coupled with automatable cell isolation methods and Single Cell Gene Expression Flex-enabled multiplexed single cell sequencing, will usher in a new age of economical, large-scale translational research studies that result in valuable clinical insights. Citation Format: Kelly Martin, Connor Kunihiro, Jawad Abousoud, Tingsheng Drennon, Yina Li, Sean Marrache, Nandhini Raman, Veronica Rodriguez, Paul Lund, Jens Durruthy Durruthy, Sarah Taylor, Andrew Kohlway, Peter Smibert, Dagmar Walter, Jill Herschleb. Preservation of patient whole blood preserves cancer immune cell biology at single cell resolution [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 995.
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Contreras Perea, Juan Carlos, Arturo Galindo Fraga, Martha Asunción Huertas Jiménez, Aurora Muñoz Pedraza, and Juan Miguel Terán Soto. "Guía de práctica clínica para toma de muestra de gases en sangre en México." Latin american journal of clinical sciences and medical technology 4, no. 1 (July 21, 2022): 121–33. http://dx.doi.org/10.34141/ljcs6842624.

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In order to promote preanalytic blood gas sample quality and to standardize sample collection, transport, and preservation protocols, a multidisciplinary experts group developed Guía de práctica clínica para toma de muestra de gases en México (Clinical practice guidelines for blood gas sample collection in Mexico). It includes recommendations for request, identification and patient´s preparation, sampling indications, contraindications, simple type selection, anatomical site, use and selection of the optimal sampling materials, operating room sampling, complications, handling, transport, and simple rejection criteria.
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Song, Jiaojiao, and Junmei Zhou. "Effects of preservation duration at 4 °C on the quality of RNA in rabbit blood specimens." PeerJ 8 (April 10, 2020): e8940. http://dx.doi.org/10.7717/peerj.8940.

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A prolonged preservation duration of blood specimens at 4 °C may occur due to the distance from collection points to storage facilities in many biobanks, especially for multicenter studies. This could lead to RNA degradation, affecting downstream analyses. However, effects of preservation durations at 4 °C on RNA quality in blood specimens need to be studied. We collected rabbit blood using EDTA tubes and stored them at 4 °C for different preservation durations. Then, we examined the quality of RNA from whole blood and leukocytes isolated from rabbit blood. Our results show that the purity of whole blood RNA and leukocyte RNA does not indicate significant change after rabbit blood is stored at 4 °C for different preservation durations (from 1 h to 7 days). The integrity of leukocyte RNA indicates the same result as above, but the integrity of whole blood RNA is significantly decreased after rabbit blood is stored at 4 °C for over 3 days. Moreover, expression of SMAD7, MKI67, FOS, TGFβ1 and HIF1α of whole blood RNA and leukocyte RNA remains basically stable, but PCNA expression of whole blood RNA or leukocyte RNA is significantly decreased after rabbit blood is stored at 4 °C for over 24 h or 7 days. Therefore, these results suggest that high-quality RNA is obtained from the fresher blood specimens and if blood specimens are stored for over 3 days at 4 °C, the quality of leukocyte RNA is more stable and of better quality than that of whole blood RNA.
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Nurunabi, Abu Sadat Mohammad, Miliva Mozaffor, Mohammad Tipu Sultan, Md Mozaharul Islam, and Kaisar Haroon. "Utilization of Brain Tissue as A Viable Postmortem Toxicological Specimen: A Review on Collection and Preservationof Samplefor Toxicological Analysis and Its Advantage Over Other Specimens." Bangladesh Journal of Neurosurgery 11, no. 2 (September 7, 2022): 114–17. http://dx.doi.org/10.3329/bjns.v11i2.61455.

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Collection of proper autopsy specimen and preservation are essential stepsfor the toxicological analysis in Forensic Medicine. Faulty collection and preservation of the specimens/samples can greatly alter or negate forensic chemical or toxicologicalexamination. In forensic toxicology practicein Bangladesh, postmortem specimen that is subjected to toxicological examinations generally focusing on mainly blood and sometimes urine or other fuds from different body cavities. Analysis of blood from different anatomical sites and tissue samples and urine may assist in the interpretation of the postmortem results. However, in many postmortem cases, there is little or no blood for quantitative drug analysis, or there might be such traumatic injury which led to significant blood loss or there is possibility of contamination form contents of the ruptured stomach. Besides, analysis of urine reveals negative result, if death occurs closely the time of intoxication. Given the circumstances, brain tissue may be a valuable specimen in postmortem toxicological analysis. The position of the brain in the body secures a tremendous protection and isolation which can eliminates or at least attenuates many of the interpretive challenges with postmortem blood, urine or other fluid specimens.This review paper is an update on the standard methods of brain tissue specimen collection and preservationprocedures for toxicological analysis and its value as well as advantages over other specimens, which might be of possible interest for forensic professionals in the country. Bang. J Neurosurgery 2022; 11(2): 114-117
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Dissertations / Theses on the topic "Blood – Collection and preservation"

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Leroy, Stephanie A. "College students' knowledge of blood donation." Virtual Press, 1998. http://liblink.bsu.edu/uhtbin/catkey/1115747.

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The purpose of this study was to determine the knowledge of college students with regard to blood donation in order to be able to create an education program to recruit new donors. After creating a table of specifications, a questionnaire was designed and reviewed by a jury of experts, and then tested in a pilot study. In the final study, 782 usable questionnaires were completed; the majority of students from the convenience sample were female (60.9%), under the age of 21 (93.1%), white (86.2%), non-Hispanic (95.8%), and had earned some college credits (61.4%).The data were analyzed using mean, t-tests, and ANOVA to test five null hypotheses. The overall knowledge (60%) of the subjects was less (M = 13.11 out of a possible 22) than anticipated. Statistically significant differences in knowledge of blood donation was found between college males and females (p < 0 .028), among students by education level (p < 0.047), and among students who were frequent, occasional, and nondonors (p < 0.000). No difference was found in the knowledge of blood donation among students by age.
Department of Physiology and Health Science
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Ouyang, Jian, and 欧阳剑. "Characteristics of blood donors and factors associated with blood donation in Guangzhou." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/206960.

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Objective: To describe and compare the characteristics of blood donors and non-donors and to examine factors associated with donation, including motivators and barriers of blood donation in Guangzhou, China. Design: Cross-sectional survey using self-administered standardized structured questionnaires on both donors and non-donors. Setting: 12 mobile and 4 permanent blood donation stations in Guangzhou during the whole operation time. Participants: 500 blood donors who donated at the donation sites and 500 non-donors who never donated and passed by the station were asked to complete a self-administered questionnaire during Dec 10, 2013 to Jun 25, 2014. Main outcome measures: Blood donation or no donation. Results: 1080 questionnaires were collected, of which 1034(95.7%) questionnaires were valid. 602(58.2%) participants were donors and 432(41.8%) were non-donors. Older people (OR: 1.46, 95% confidence interval: 1.24 to 1.72, p<0.01), males (1.33, CI: 1.02 to 1.71, p=0.03), non-college-students (1.76, CI: 1.16 to 2.56, p<0.01) and people with higher education level (1.27, CI: 1.11 to 1.45, p<0.01) were more likely to be donors. The main objective of blood donation was helping patients (n=405, 68.2%), and the main reason of not donating was being in poor health (n=138, 33.1%). However, other motives, such as benefiting health and free check for blood type and body, and obstacles, such as failing to meet the requirements and fear, were also important. More male donors would donate again than females (80.5% vs. 68.5%, p<0.01), whereas more female donors showed uncertainty than males (25.9% vs. 16.6%, p<0.01). Usage of blood (n=182, 46.7%) was what non-donors wanted to know the most if they were to donate in the future. The majority of participants (n=730, 71.3%) considered raising the awareness of blood donation among people was one of the most effective ways of blood donation promotion. Television was considered as one of the most effective methods of blood donation promotion and recruitment, and was more acceptable to females. Younger participants preferred the internet. Conclusion: These findings suggest that raising the awareness of blood donation is vital. Campaigns should focus on multiple aspects targeting different groups of people. Television and the internet are useful tools of blood donation promotion and recruitment.
published_or_final_version
Public Health
Master
Master of Public Health
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Adam, Douglas. "An investigation of a theoretical model of willingness to donate blood." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 1997. https://ro.ecu.edu.au/theses/899.

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The Australian Red Cross Blood Transfusion Service (ARCBTS) in Western Australian faces a major problem with periodic shortages of blood components. These shortages are expected to become more frequent and severe as demand continues to increase at a faster rate than supply. Given that only five percent of the population is registered as blood donors, clearly, the challenge for the ARCBTS is to encourage more people to become regular blood donors. The current study was undertaken to assist the ARCBTS in achieving this goal, by identifying and investigating the factors that influence people's willingness to donate blood. Based on the findings of a literature review and focus groups, a conceptual model of "willingness to donate blood" was developed. The model included personal values, knowledge about blood donation, perceived risks associated with donating blood, and attitudes towards blood donation, as antecedents to willingness to donate.
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Harris, Maryke. "Deterrents to continued blood donation among regular blood donors." Thesis, Nelson Mandela Metropolitan University, 2017. http://hdl.handle.net/10948/15934.

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Collecting blood from repeat blood donors is cost effective and safer compared to other donor types. At the end of 2012, 84% of the SANBS donor panel were inactive or lapsed. There is a lack of research available on lapsed donors in the South African context and available research is mostly quantitative with subtle contradictions. Donors who donated blood in 2012 at fixed site donor centres in Port Elizabeth, and did not return in 2013, were studied. A descriptive analysis was done and a random sample of 78 lapsed donors were selected to participate in a face-to-face interview. Interviews were digitally recorded and transcribed. A grounded model was developed from various existing theories to seek out and conceptualise social patterns and structures of lapsed blood donors through a process of comparison. There were 10 062 donors who donated blood in 2012 and 4 923 became lapsed during 2013. Analysis of sub groups showed a higher proportion of donors who became lapsed in the following sub-categories: new donors (95%), re-joined donors (64%), black donors (63%), donors younger than 40 (61%), female donors (52%). The feedback received from the 11 participants highlighted peer pressure as the biggest motivator. Of the six communication theories applied, The Social Penetration Theory highlighted the cost-minus-benefit ratio which played a big role in a donor’s motivation and decision to return. The AIDA Marketing Model application described lapsed donor behaviour most comprehensively and it highlighted a missing step which was created as part of a Grounded Model and is called the AIDAA Model. The role and existence of peer pressure is directly linked to donor motivation and is categorised as an Action Motivator in the AIDAA Model. The new model creates additional recruitment opportunities which has not been explored and applied strategically before.
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Prado, Eric A. "Measuring Biomarkers From Dried Blood Spots Utilizing Bead-based Multiplex Technology." Thesis, University of North Texas, 2014. https://digital.library.unt.edu/ark:/67531/metadc699876/.

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Dried blood spots is an alternative method to collect blood samples from research subjects. However, little is known about how hemoglobin and hematocrit affect bead-based multiplex assay performance. The purpose of this study was to determine how bead-based multiplex assays perform when analyzing dried blood spot samples. A series of four experiments outline the study each with a specific purpose. A total of 167 subject samples were collected and 92 different biomarkers were measured. Median fluorescence intensity results show a positive correlation between filtered and non-filtered samples. Utilizing a smaller quantity of sample results in a positive correlation to a larger sample. Removal of hemoglobin from the dried blood spot sample does not increase detection or concentration of biomarkers. Of the 92 different biomarkers measured 56 were detectable in 100-75% of the attempted samples. We conclude that blood biomarkers can be detected using bead-based multiplex assays. In addition, it is possible to utilize a smaller quantity of sample while avoiding the use of the entire sample, and maintaining a correlation to the total sample. While our method of hemoglobin was efficient it also removed the biomarkers we wished to analyze. Thus, an alternative method is necessary to determine if removing hemoglobin increases concentration of biomarkers. More research is necessary to determine if the biomarkers measured in this study can be measured over time or within an experimental model.
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Adams, Faieqa. "An in vitro comparison of cellular destruction and metabolic effects occurring in stored, leuco-reduced and irradiated red blood cells." Thesis, Cape Peninsula University of Technology, 2016. http://hdl.handle.net/20.500.11838/2457.

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Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2016.
Biochemical and haematological changes occur in red blood cellular products during the recommended storage period of 35 to 42 days at 1°C to 6°C. The restriction of the sodium/potassium pump at specified temperatures result in low intracellular potassium ion levels while an increase in sodium ion levels are observed and acidosis occurs as a result of low pH concentrations due to glucose consumption. Structural and morphological changes occur such as the release of free haemoglobin, lactate dehydrogenase and potassium into the supernatant causing the formation of spheroechinocytes and osmotic fragility. All these factors negatively impact the rheological properties of blood. These changes that transpire in the red cells during the storage period are referred to as “storage lesions”. Transfusion-associated graft versus host disease is an immunological and often fatal adverse transfusion reaction with gamma irradiation of cellular blood products used as a preventative measure. Gamma irradiation exacerbates storage lesions and of particular concern has been the increased potassium levels resulting in neonatal and infant hyperkalaemia. The storage lesions occurring in non-irradiated red blood cellular products are well documented although the literature regarding its irradiated counterparts has been less studied. A study of this nature has not previously been done in Cape Town, South Africa.
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Garcia, Claudia Zeferino. "Estabilidade do fator de von Willebrand e fator VIII no crioprecipitado canino em diferentes protocolos de armazenamento /." Botucatu, 2014. http://hdl.handle.net/11449/124094.

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Orientador: Regina Kiomi Takahira
Banca: Luiz Henrique de Araújo Machado
Banca: Simone Gonçalves Rodrigues Gomes
Resumo: O fator VIII (FVIII), o fator de von Willebrand (FvW) e o fibrinogênio são de suma importância na coagulação sanguínea, com diferentes funções fisiológicas. Por conter altas concentrações destes fatores a transfusão de crioprecipitado é uma terapia utilizada principalmente em pacientes que apresentam Doença de von Willebrand, Hemofilia A (deficiência do FVIII), ou pacientes que sofrem de hipo ou disfibrinogenemia. Este hemocomponente é um precipitado obtido após o descongelamento parcial (entre 1 e 6°C) do plasma fresco congelado, e também é conhecido como fator anti-hemofílico. Estudos têm demonstrado que o protocolo de congelamento e armazenamento do crioprecipitado afeta a qualidade do produto e a viabilidade destes fatores. Com o objetivo de avaliar a viabilidade do crioprecipitado canino em diferentes protocolos de congelamento e armazenamento foram avaliados dois grupos compostos de 10 unidades de crioprecipitado canino (n=20). Após a centrifugação das bolsas de sangue, o plasma fresco foi congelado a -80ºC (grupo I) e a -20ºC (grupo II). Vinte e quatro horas após o congelamento das bolsas, estas foram submetidas ao procedimento de extração do crioprecipitado. Os crioprecipitados das bolsas dos dois grupos foram submetidos à determinação do TP, TTPA, FVIII, FvW e fibrinogênio, no momento zero e após seis meses de estocagem. Para a realização das coletas, foram utilizadas bolsas sanguíneas triplas de plástico, com anticoagulante CPDA-1, sendo a bolsa principal com capacidade para 450 mL de sangue total (JP Indústria Farmacêutica®). Após o crioprecipitado devidamente pronto, uma alíquota de aproximadamente 5 mL da bolsa de crioprecipitado foi separada em criotubos para análise da amostra pré-estocagem e seis meses pós estocagem. As amostras obtidas em cada momento foram congeladas à -80ºC até o momento do processamento. Os resultados mostraram um decréscimo significativo dos fatores e ...
Abstract: The factor VIII (FVIII), the von Willebrand factor (vWF) and the fibrinogen are extremely important to the blood clotting process, with various physiological functions. Because it contains high concentrations of these factors and fibrinogen, transfusing cryoprecipitate is a therapy mainly used in patients who have von Willebrand disease, Hemophilia A (FVIII deficiency), or who suffer from hypo/dysfibrinogenemia. This hemocomponent is a precipitate obtained after the partial thawing process (between 1 and 6ºC) of fresh frozen plasma, and which is also known as the anti-hemophilic factor. Studies have demonstrated that the cryoprecipitate freezing and storage protocol affects the product quality as well as these factors viability. In order to evaluate the canine cryoprecipitate viability in different freezing and storage protocols, two groups containing 10 units of canine cryoprecipitate (n=20) were evaluated. Following the blood centrifugation, the fresh plasma was frozen at -80ºC (group I) and at -20ºC (group II). Twenty-four hours after freezing the blood bags, they were submitted to the cryoprecipitate extraction procedure. The cryoprecipitate from both groups of blood bags were submitted to the TP, TTPA, FVIII, FvW and fibrinogen determination process, at time zero and after six months of storage. During the collections, triple plastic blood bags were used, along with the anticoagulant CPDA-1, being the main bag capacity of 450 mL of whole blood (JP Indústria Farmacêutica®). After having the cryoprecipitate properly ready, an approximately 5 mL aliquot of cryoprecipitate was separated into cryovials to be analysed pre-storage and six months after storage. However, there was no significant difference between treatments, demonstrating that the difference in initial freezing temperature did not influence the decrease of the factors after six months storage at -20°C
Mestre
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Garcia, Claudia Zeferino [UNESP]. "Estabilidade do fator de von Willebrand e fator VIII no crioprecipitado canino em diferentes protocolos de armazenamento." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/124094.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O fator VIII (FVIII), o fator de von Willebrand (FvW) e o fibrinogênio são de suma importância na coagulação sanguínea, com diferentes funções fisiológicas. Por conter altas concentrações destes fatores a transfusão de crioprecipitado é uma terapia utilizada principalmente em pacientes que apresentam Doença de von Willebrand, Hemofilia A (deficiência do FVIII), ou pacientes que sofrem de hipo ou disfibrinogenemia. Este hemocomponente é um precipitado obtido após o descongelamento parcial (entre 1 e 6°C) do plasma fresco congelado, e também é conhecido como fator anti-hemofílico. Estudos têm demonstrado que o protocolo de congelamento e armazenamento do crioprecipitado afeta a qualidade do produto e a viabilidade destes fatores. Com o objetivo de avaliar a viabilidade do crioprecipitado canino em diferentes protocolos de congelamento e armazenamento foram avaliados dois grupos compostos de 10 unidades de crioprecipitado canino (n=20). Após a centrifugação das bolsas de sangue, o plasma fresco foi congelado a -80ºC (grupo I) e a -20ºC (grupo II). Vinte e quatro horas após o congelamento das bolsas, estas foram submetidas ao procedimento de extração do crioprecipitado. Os crioprecipitados das bolsas dos dois grupos foram submetidos à determinação do TP, TTPA, FVIII, FvW e fibrinogênio, no momento zero e após seis meses de estocagem. Para a realização das coletas, foram utilizadas bolsas sanguíneas triplas de plástico, com anticoagulante CPDA-1, sendo a bolsa principal com capacidade para 450 mL de sangue total (JP Indústria Farmacêutica®). Após o crioprecipitado devidamente pronto, uma alíquota de aproximadamente 5 mL da bolsa de crioprecipitado foi separada em criotubos para análise da amostra pré-estocagem e seis meses pós estocagem. As amostras obtidas em cada momento foram congeladas à -80ºC até o momento do processamento. Os resultados mostraram um decréscimo significativo dos fatores e ...
The factor VIII (FVIII), the von Willebrand factor (vWF) and the fibrinogen are extremely important to the blood clotting process, with various physiological functions. Because it contains high concentrations of these factors and fibrinogen, transfusing cryoprecipitate is a therapy mainly used in patients who have von Willebrand disease, Hemophilia A (FVIII deficiency), or who suffer from hypo/dysfibrinogenemia. This hemocomponent is a precipitate obtained after the partial thawing process (between 1 and 6ºC) of fresh frozen plasma, and which is also known as the anti-hemophilic factor. Studies have demonstrated that the cryoprecipitate freezing and storage protocol affects the product quality as well as these factors viability. In order to evaluate the canine cryoprecipitate viability in different freezing and storage protocols, two groups containing 10 units of canine cryoprecipitate (n=20) were evaluated. Following the blood centrifugation, the fresh plasma was frozen at -80ºC (group I) and at -20ºC (group II). Twenty-four hours after freezing the blood bags, they were submitted to the cryoprecipitate extraction procedure. The cryoprecipitate from both groups of blood bags were submitted to the TP, TTPA, FVIII, FvW and fibrinogen determination process, at time zero and after six months of storage. During the collections, triple plastic blood bags were used, along with the anticoagulant CPDA-1, being the main bag capacity of 450 mL of whole blood (JP Indústria Farmacêutica®). After having the cryoprecipitate properly ready, an approximately 5 mL aliquot of cryoprecipitate was separated into cryovials to be analysed pre-storage and six months after storage. However, there was no significant difference between treatments, demonstrating that the difference in initial freezing temperature did not influence the decrease of the factors after six months storage at -20°C
FAPESP: 2012/13677-6
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Elliott, Jennifer. "Studies on the preservation of flowers." Thesis, University of St Andrews, 2002. http://hdl.handle.net/10023/2693.

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A known method for the preservation of green foliage was adapted in order to preserve floral tissues, retaining the colour and texture, thereby providing a method suitable for the preservation of whole flowers. Initially, the effects of the existing foliage preservation process on floral tissues were studied and the resulting problems of limp sticky petals and colour loss were identified. Subsequently, with a knowledge of basic plant anatomy and of the properties of the main floral pigments, the anthocyanins, a series of experiments on petals and whole flowers were carried out in an attempt to rectify these problems and to incorporate the remedies into a method for preserving whole flowers. The problem of improving the texture and firmness of flower heads was tackled by investigating the effects of adding bulking or setting ingredients to the process fluid and establishing their optimum concentrations. In the case of flower colour, the addition of acid was required in order to maintain the bright anthocyanin colours and a range of acids was investigated. Furthermore, since it is known that in nature the anthocyanin pigments are stabilised by metal ions and copigments, the use of these agents in the preservation process was also considered. This empirical work was then validated by confirming the identity of the main pigments involved and by studying various aspects of the new preservation process. Factors examined included acid concentration, temperature, solvent composition and the addition of metal ions and copigments to solutions of petal extracts containing anthocyanin pigments. Physical changes resulting from processing, including process fluid content and the moisture absorption properties of processed petals were also measured. Finally, the application of a selection of coating materials was assessed in an attempt to increase the life span of the processed flowers by providing extra protection against environmental stresses.
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Vernoski, Barbara K. "Effect of Blood Collection Practices on Emergency Department Blood Specimen Rejection Rates." UNF Digital Commons, 2013. http://digitalcommons.unf.edu/etd/438.

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The practice of obtaining blood as part of the placement of a new peripheral venous access device (p-VAD) is a frequent practice in the emergency department (ED). Of the concerns related to this practice is the possibility of laboratory specimen rejection due to p-VAD catheter size, use of the wrong collection device, and the absence of a standardized collection process. The objective of this study, therefore, was to examine the effect of the use of evidence-based venipuncture and p-VAD blood collection protocols on the rejection rate of blood specimens drawn by staff in the adult areas of an urban academic medical center ED. A convenience sample of 28 ED nurses and 39 ED technicians (51.94% of all eligible ED employees) consented to using these evidence based protocols when they collected blood from adult ED patients. Blood specimen rejections rates were measured for four consecutive weeks prior to and at weeks 1-4, 5-8, 9-12, and 1-12 after the evidence-based blood collection practices training intervention. Laboratory analysis of all specimens was automated with rejection results provided in the form of computerized reports. There was a significant decrease in the 12-week rejection rates for two of the three ED adult care areas, with the overall ED adult area rejection rate significantly decreased from 3.19% to 2.38% (X2at Df1, p < .05). The most common reasons for rejection were hemolysis (65.39%) and clotting (10.68%) followed by specimen mis-labeling, tube missing, insufficient quantity for testing, incorrect packaging, specimen contamination or dilution, and label missing, Though the use of theses evidence based blood collection protocols significantly decreased the overall rejection rate, the high percent of rejections due to hemolysis may further be reduced by having all ED staff use these protocols, and by exploring other collection techniques in the literature that have been found to significantly decrease rejection rates.
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Books on the topic "Blood – Collection and preservation"

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J, Manning Frederick, and Sparacino Linette R, eds. Blood donors and the supply of blood and blood products. Washington, D.C: National Academy Press, 1996.

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Orfinger, Rebecca. Blood 101: The fundamentals of blood safety. Edited by American Red Cross. [Washington, DC]: American Red Cross, 2001.

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Algiere, Kasprisin Christina, and Laird-Fryer Barbara, eds. Blood donor collection practices. Bethesda, Md: American Association of Bolld Banks, 1993.

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National Blood Data Center (U.S.). The Nation's blood resource: A summary report. [Bethesda, Md.?]: U.S. Department of Health and Human Services, Public Health Service, National Institutes of Health, 1985.

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Gian, Alicia. Blood 101: One hundred and one reasons to donate blood. Garden City, KS: Alicia Gian, 2003.

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Garza, Diana. Phlebotomy handbook: Blood collection essentials. 6th ed. Upper Saddle River, N.J: Prentice Hall, 2002.

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Garza, Diana. Phlebotomy handbook: Blood collection essentials. 6th ed. Upper Saddle River, N.J: Prentice Hall, 2002.

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Garza, Diana. Phlebotomy handbook: Blood collection essentials. 6th ed. Upper Saddle River, NJ: Prentice Hall, 2002.

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King, Strasinger Susan, and Di Lorenzo, Marjorie Schaub, 1953-, eds. Blood collection: A short course. 2nd ed. Philadelphia: F.A. Davis, 2009.

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Surgenor, Douglas M. The nation's blood resource: A summary report. [Bethesda, Md.?]: U.S. Dept. of Health and Human Services, Public Health Service, National Institutes of Health, 1985.

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Book chapters on the topic "Blood – Collection and preservation"

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Kumar, Vijay, and Kiran Dip Gill. "Blood Collection and Preservation." In Basic Concepts in Clinical Biochemistry: A Practical Guide, 5–7. Singapore: Springer Singapore, 2018. http://dx.doi.org/10.1007/978-981-10-8186-6_2.

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Distler, Jurgen, Reimo Tetzner, Gunter Weiss, Thomas König, Anne Schlegel, and Michal Bagrowski. "Evaluation of Different Blood Collection Tubes and Blood Storage Conditions for the Preservation and Stability of Cell-Free Circulating DNA for the Analysis of the Methylated mSEPT9 Colorectal Cancer Screening Marker." In Advances in Experimental Medicine and Biology, 175–78. Cham: Springer International Publishing, 2016. http://dx.doi.org/10.1007/978-3-319-42044-8_32.

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Chaplin, H., and P. L. Mollison. "Preservation of Blood." In Novartis Foundation Symposia, 121–30. Chichester, UK: John Wiley & Sons, Ltd., 2008. http://dx.doi.org/10.1002/9780470718889.ch9.

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Škaloud, Pavel, Fabio Rindi, Christian Boedeker, and Frederik Leliaert. "Collection, preservation and culturing." In Freshwater Flora of Central Europe, Vol 13: Chlorophyta: Ulvophyceae (Süßwasserflora von Mitteleuropa, Bd. 13: Chlorophyta: Ulvophyceae), 25–26. Berlin, Heidelberg: Springer Berlin Heidelberg, 2018. http://dx.doi.org/10.1007/978-3-662-55495-1_7.

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Smith, Clifford, and Alfred Jarecki. "Blood Collection Procedures." In Atlas of Comparative Diagnostic and Experimental Hematology, 105–6. West Sussex, UK: John Wiley & Sons, Ltd., 2013. http://dx.doi.org/10.1002/9781118785072.ch7.

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Ajmani, Pritam Singh. "Donor Blood Collection." In Immunohematology and Blood banking, 25–35. Singapore: Springer Singapore, 2020. http://dx.doi.org/10.1007/978-981-15-8435-0_3.

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Yagi, Kenichiro. "Canine Blood Collection." In Manual of Veterinary Transfusion Medicine and Blood Banking, 199–211. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2016. http://dx.doi.org/10.1002/9781118933053.ch14.

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Taylor, Robyn K., and Karen Humm. "Feline Blood Collection." In Manual of Veterinary Transfusion Medicine and Blood Banking, 223–36. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2016. http://dx.doi.org/10.1002/9781118933053.ch16.

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Costa, Lais R. R., and Ann Chapman. "Venous Blood Collection." In Manual of Clinical Procedures in the Horse, 59–66. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781118939956.ch4.

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Costa, Lais R. R. "Arterial Blood Collection." In Manual of Clinical Procedures in the Horse, 67–71. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2017. http://dx.doi.org/10.1002/9781118939956.ch5.

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Conference papers on the topic "Blood – Collection and preservation"

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Kurnikova, Irina, Ramchandra Sargar, Alexi Pavlov, Zhanar Baisenbaeva, Natalia Zabrodina, and Maria Zavalina. "Quantitative assessments in evaluating the effectiveness of arterial hypertension treatment: new technologies." In 9th International Conference on Human Interaction and Emerging Technologies - Artificial Intelligence and Future Applications. AHFE International, 2023. http://dx.doi.org/10.54941/ahfe1002959.

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Background. In the practice of clinical research, it is traditionally accepted to evaluate parameters that characterize the degree of impairment or loss of the function of an organ or system, and not their preservation. A fundamental change in the approach distinguishes a new trend in modern medicine, which makes it possible to assess the degree of preservation of functional resources, which was the basis of this study.Study purpose: To develop a method for dynamic quantitative assessment of the severity of arterial hypertension and evaluate the relationship between a qualitative indicator of arterial hypertension and a quantitative indicator.Instruments and Data Collection Procedure. Index of adaptive aptitude (IAA) (patent for invention No. 2342900 "Method for eavaluation of the functional reserves of the body", author - Kurnikova I. A.). using automated assessment (certificates of official registration No. 2007614560 and No. 2007613898).IAA=0.011(P-P*)+0.014(S-S*)+0.008(D-D*)+0.009(W-W*),Where: P - actual heart rate (b/min.); P* - ideal pulse rate (beats/ min.); S - systolic blood pressure, actual average per day (mm Hg); S* – ideal systolic blood pressure (mm Hg); D - diastolic blood pressure, actual average per day (mm Hg); D* - ideal diastolic blood pressure (mm/Hg); W - body weight at the time of examination (kg); W* - ideal body weight (kg); H - patient's height at the time of examination (cm).Assessment of daily heart rate variability on the Valenta system, equipped with a program for computer processing of spectral analysis indicators.Results:- 143 patients with arterial hypertension (AH) were examined. The relationship between a qualitative indicator - the severity of AH and quantitative indicators - rehabilitation potential, the numerical value of which is PAS, the circadian index (CI) as an indicator of increased sensitivity of the heart rate to sympathetic stimulation, and the LF/HF vago sympathetic balance coefficient, which increases with hypersympathatic tone, was considered. Statistical analysis of the surface plot using the weighted least squares distance allowed this relationship to be clearly demonstrated. Quantitative analogue of the Framingham arterial hypertension severity scale (AHSS) in a specific patient (certificate for invention №. 201152181)AHSS = 119,31 + 2,22× (CI) - 2,03× (IAA) - 1,33×(2×(CI)) + 2,72×(CI)× (IAA) + 7,06×(IAA)where: AHSS less than 120 - normal. Mild AHSS = 121 – 130 points; average severity of AHSS = 131 – 140 points; severe AHSS - 141 points or more. The effectiveness of rehabilitation is good with AHSS less than 120, satisfactory - with 121 - 130; unsatisfactory - more than 131.For illustration, the data of 54 patients are presented. Observation group "1" - patients with AH (BMI<25), mean age - 49±1.9 years (19 people). Observation group "2" - patients with metabolic syndrome (BMI>25; HOMA index> 2.5), mean age - 50±1.7 (35 people). All patients were assessed for the severity of hypertension according to the WHO criteria and the AHSS index at the beginning of the study. Normal value was achieved in 41.8% in 1st group during treatment, in 47.1% there was a decrease in the severity of AH according to AHSS. In group 2, in patients with metabolic syndrome during treatment, it was possible to normalize blood pressure in 55.8%, and to obtain satisfactory results in 35.9%.Conclusion. Developed mathematical modeling methods, a quantitative analogue indicator of the severity of arterial hypertension - AHSS allows you to dynamically monitor the effectiveness of treatment and evaluate the achieved result based on a quantitative assessment within the severity indicated by the classification. The effectiveness of the rehabilitation of patients with hypertension is considered sufficient if the AHSS decreases below the value of 120 units or one level from the baseline
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Adelstein, Peter Z., and William D. Storm. "Standardization efforts for the preservation of electronic imagery." In Critical Review Collection. SPIE, 1991. http://dx.doi.org/10.1117/12.48896.

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Zhang, Yilin. "Collection and Preservation of Electronic Evidence." In 3rd International Conference on Science and Social Research (ICSSR 2014). Paris, France: Atlantis Press, 2014. http://dx.doi.org/10.2991/icssr-14.2014.320.

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Ng, Wee Siong, Huayu Wu, Wei Wu, Shili Xiang, and Kian-Lee Tan. "Privacy Preservation in Streaming Data Collection." In 2012 IEEE 18th International Conference on Parallel and Distributed Systems (ICPADS). IEEE, 2012. http://dx.doi.org/10.1109/icpads.2012.132.

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Dayong, Huo, and Luo Gangyin. "Design of automatic blood collection scale system and study on measurement method of blood collection velocity." In 2023 2nd International Conference on Health Big Data and Intelligent Healthcare (ICHIH). IEEE, 2023. http://dx.doi.org/10.1109/ichih60370.2023.10396274.

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Pearce, John A., and Sharon L. Thomsen. "Blood vessel architectural features and their effects on thermal phenomena." In Critical Review Collection. SPIE, 2000. http://dx.doi.org/10.1117/12.375226.

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Li, Xi, Jun Li, Wenzhu Wu, and Ting Wang. "Research on Intelligent Fingertip Blood Collection Robot." In ISAIMS 2021: 2nd International Symposium on Artificial Intelligence for Medicine Sciences. New York, NY, USA: ACM, 2021. http://dx.doi.org/10.1145/3500931.3500963.

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Taerakul, Tarit, Krit Pongpirul, Sathit Niramitmahapanya, Ithirit Chaowaleard, Panida Yuphet, and Krisana Arsayot. "Cost Analysis of the Blood Collection at the Patient’s Home Compared with the Blood Collection at the Hospital." In 4th International Conference on Public Health and Well-being. iConferences (Pvt) Ltd, 2023. http://dx.doi.org/10.32789/publichealth.2022.1010.

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The cost data of the home blood collection project was compared with the current blood collection service. Using direct and indirect cost data from full cost data, including output data for the number of outpatient services, and assessing the cost per unit cost or average cost of services together with the standard costing method of calculating the cost of medical services. Only the cost per unit of medical technology services, medical record and statistics services, and finance and accounting services were calculated. The cost per visit for blood collection services was 21.37 USD. The direct non-medical costs of the blood collection service at the hospital were 30.62 USD. The cost structure of the blood collection at the hospital is mostly fixed (95% of the total cost) and only 5 percent is variable, while the home service can save the cost by about 50% (the cost at the hospital is 30.62 USD, compared to 15.34 USD at home). So, the blood collection at home can reduce the patient's cost burden by 50 percent and the hospital’s cost by 5 percent, as well as being able to respond to the policy of reducing congestion in hospitals during the pandemic situation. Keywords: home lab service, Rajavithi Hospital, new normal, cost analysis
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Browne, Cecille, Dan Lu, Daniela Roth, and Vasco Liberal. "Abstract 401: Preservation of cells in blood and culture." In Proceedings: AACR 107th Annual Meeting 2016; April 16-20, 2016; New Orleans, LA. American Association for Cancer Research, 2016. http://dx.doi.org/10.1158/1538-7445.am2016-401.

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Yu, Fang. "Case Study of Digital Preservation for E-heritage: Digital Preservation Project for the Collection of Penhas Family." In 2010 Fourth International Conference on Mangement of E-Commerce and E-Government (ICMeCG). IEEE, 2010. http://dx.doi.org/10.1109/icmecg.2010.23.

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Reports on the topic "Blood – Collection and preservation"

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Moore, Gerald L. Long-Term Storage and Preservation of Red Blood Cells,. Fort Belvoir, VA: Defense Technical Information Center, January 1992. http://dx.doi.org/10.21236/ada257994.

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Williams, M. Handbook for sample collection, preservation, and instrumental techniques. Office of Scientific and Technical Information (OSTI), February 1990. http://dx.doi.org/10.2172/5133090.

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Lotti, R. Accession, curation, and preservation of a deep-sea sample collection. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1994. http://dx.doi.org/10.4095/193919.

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Moore, G. L., and M. E. Ledford. Effects of Buffered Deglycerolization Solutions on the 21-Day Post-Thaw Preservation of Red Blood Cells. Fort Belvoir, VA: Defense Technical Information Center, March 1993. http://dx.doi.org/10.21236/ada266498.

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Edwards, Ryan. If My Blood Pressure Is High, Do I Take It To Heart? Behavioral Impacts of Biomarker Collection in the Health and Retirement Study. Cambridge, MA: National Bureau of Economic Research, August 2013. http://dx.doi.org/10.3386/w19311.

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Kozhevnikova, O. A. ELECTRONIC COLLECTION OF TEST TASKS FOR THE COURSE "FUNDAMENTALS OF PEDIATRICS AND HYGIENE" : A BANK OF QUESTIONS. SIB-Expertise, January 2022. http://dx.doi.org/10.12731/er0530.21012022.

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The test tasks are compiled in accordance with the requirements of the Federal State Educational Standard in the direction of "Psychological and pedagogical education" and are designed to control the formation of the following universal general professional and professional competencies: OPK-1 (Able to carry out professional activities in accordance with regulatory legal acts in the field of education and professional ethics) and PC-6 (Capable of ensuring the protection of the life and health of students). Indicators of competence achievement are knowledge of the priority directions of the development of the education system of the Russian Federation, laws and other regulatory legal acts regulating activities in the field of education in the Russian Federation, legislative documents on the rights of the child, the Convention on the Rights of the Child; knowledge of methods and methods of ensuring the safety of students in dangerous situations; ability to apply basic regulatory legal acts in the field of education and professional ethics; the ability to analyze and assess the degree of danger in various situations, the ability to provide conditions for a safe and comfortable educational environment that contributes to the preservation of life and health of students.
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Yeika, Eugene, Erica L. Kocher, and Carrie Ngongo. Integrating Noncommunicable Diseases into Antenatal Care in Cameroon: A Triangulated Qualitative Analysis. RTI Press, January 2024. http://dx.doi.org/10.3768/rtipress.2024.rr.0051.2401.

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Noncommunicable diseases (NCDs) have important implications for pregnancy outcomes and the subsequent health of women and their children. The aim of this study is to determine the status of NCD and maternal health program integration, identify barriers to integration, and explore what would be required to deepen integration of NCD care into antenatal care in Cameroon. We used two methods of data collection and synthesis: a desk review of policy documents and protocols and a series of key informant interviews with health system experts and managers working in public, private, and faith-based health facilities at central, regional, and district levels. Although screening for blood glucose and blood pressure occurs during antenatal care, post-diagnosis management is not well-integrated and often requires referral to specialists in higher-level health facilities. Key barriers to integration include lack of guidelines for the management of NCDs, financial constraints for facilities and patients, and shortages of health workers, medications, and supplies for laboratory investigations. Further integration of services for NCDs during pregnancy will require national guidelines backed up by system-wide strengthening of health information systems, insurance coverage, supply chain management, and human resource capacity, particularly in remote areas.
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Berube, Paul M., Scott M. Gifford, Bonnie Hurwitz, Bethany Jenkins, Adrian Marchetti, and Alyson E. Santoro. Roadmap Towards Communitywide Intercalibration and Standardization of Ocean Nucleic Acids ‘Omics Measurements. Woods Hole Oceanographic Institution, March 2022. http://dx.doi.org/10.1575/1912/28054.

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In January 2020, the US Ocean Carbon & Biogeochemistry (OCB) Project Office funded the Ocean Nucleic Acids 'omics Intercalibration and Standardization workshop held at the University of North Carolina in Chapel Hill. Thirty-two participants from across the US, along with guests from Canada and France, met to develop a framework for standardization and intercalibration (S&I) of ocean nucleic acid ‘omics (na’omics) approaches (i.e., amplicon sequencing, metagenomics and metatranscriptomics). During the three-day workshop, participants discussed numerous topics, including: a) sample biomass collection and nucleic acid preservation for downstream analysis, b) extraction protocols for nucleic acids, c) addition of standard reference material to nucleic acid isolation protocols, d) isolation methods unique to RNA, e) sequence library construction, and f ) integration of bioinformatic considerations. This report provides a summary of these and other topics covered during the workshop and a series of recommendations for future S&I activities for na’omics approaches.
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Hall, G. E. M. Cost-effective protocols for the collection, filtration an dpreservation of surface waters for detection of metals and metalloids at ppb (?g 1-1) and ppt (ng -1-1) levels phase I: evaluation of bottle type, bottle cleaning, filter and preservation technique. Natural Resources Canada/ESS/Scientific and Technical Publishing Services, 1998. http://dx.doi.org/10.4095/306940.

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Løvschal, Mette, Havananda Ombashi, Marianne Høyem Andreasen, Bo Ejstrud, Renée Enevikd, Astrid Jensen, Mette Klingenberg, Søren Munch Kristiansen, and Nina Helt Nielsen. The Protected Burial Mound ‘Store Vejlhøj’, Vinderup, Denmark: First Results. Det Kgl. Bibliotek, 2023. http://dx.doi.org/10.7146/aulsps-e.479.

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Abstract:
An archaeological excavation of the protected burial mound Store Vejlhøj in northwestern Denmark was carried out in October-November 2021. The excavation formed part of the ERC-funded research project called ANTHEA, focusing on the deep history of anthropogenic heathlands. It was conducted by Aarhus University in collaboration with Holstebro Museum and Moesgaard Museum. The aim was to test a new method of sampling pollen data from different construction stages in a burial mound and comparing them with pollen data from nearby lake sediments with a view to improving our understanding of prehistoric anthropogenic heathland dynamics. Prior to the excavation, soil cores were collected from two nearby peat sediments as well as six burial mounds (including Store Vejlhøj) within a 1 km range of Lake Skånsø, where previous pollen analyses had been carried out. Based on these preliminary corings, Store Vejlhøj was selected for further archaeological investigation. A dispensation for excavating the protected mound was granted by the Danish Palaces and Culture Agency. The excavation was based on a 5 m long trench through the barrow, moving from its foot inwards. The surface vegetation and 40 cm topsoil were removed by an excavator, after which the remainder of the trench was manually dug in horizontal layers. Observation conditions were good. The excavation revealed a series of well-defined barrow construction stages, as well as unusually wellpreserved turf structures. Only two archaeological finds could be related to the barrow, both of which were later than its initial construction: a secondary urn in the top layer, and the base of a second urn at the foot of the mound. The burial mound was constructed using a minimum of three shells, which could be observed in the trench profile. Turfs were most probably collected locally in a landscape dominated by grass pastures, where no previous turf cutting had taken place. A total of 34 soil samples were collected for paleoecological analyses (pollen, Non-Pollen Polymorphs (NPPs), macrofossils) and geoarchaeological analyses (micromorphology, bulk samples). Preliminary pollen and macrofossil results from the burial mound revealed poor preservation conditions, which prompted a trench extension of 0.5 m by 0.2 m to find better preservation conditions. This extension resulted in the collection of a single final macrofossil sample, although there was no identifiable change in the in-situ preservation conditions. The dating results of the mound have not yet been completed and will be included as appendix 4-6 in 2023.
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