To see the other types of publications on this topic, follow the link: Blood – Collection and preservation.
Dissertations / Theses on the topic 'Blood – Collection and preservation'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 dissertations / theses for your research on the topic 'Blood – Collection and preservation.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse dissertations / theses on a wide variety of disciplines and organise your bibliography correctly.
1
Leroy, Stephanie A. "College students' knowledge of blood donation." Virtual Press, 1998. http://liblink.bsu.edu/uhtbin/catkey/1115747.
Full textAbstract:
The purpose of this study was to determine the knowledge of college students with regard to blood donation in order to be able to create an education program to recruit new donors. After creating a table of specifications, a questionnaire was designed and reviewed by a jury of experts, and then tested in a pilot study. In the final study, 782 usable questionnaires were completed; the majority of students from the convenience sample were female (60.9%), under the age of 21 (93.1%), white (86.2%), non-Hispanic (95.8%), and had earned some college credits (61.4%).The data were analyzed using mean, t-tests, and ANOVA to test five null hypotheses. The overall knowledge (60%) of the subjects was less (M = 13.11 out of a possible 22) than anticipated. Statistically significant differences in knowledge of blood donation was found between college males and females (p < 0 .028), among students by education level (p < 0.047), and among students who were frequent, occasional, and nondonors (p < 0.000). No difference was found in the knowledge of blood donation among students by age.Department of Physiology and Health Science
2
Ouyang, Jian, and 欧阳剑. "Characteristics of blood donors and factors associated with blood donation in Guangzhou." Thesis, The University of Hong Kong (Pokfulam, Hong Kong), 2013. http://hdl.handle.net/10722/206960.
Full textAbstract:
Objective: To describe and compare the characteristics of blood donors and non-donors and to examine factors associated with donation, including motivators and barriers of blood donation in Guangzhou, China.
Design: Cross-sectional survey using self-administered standardized structured questionnaires on both donors and non-donors.
Setting: 12 mobile and 4 permanent blood donation stations in Guangzhou during the whole operation time.
Participants: 500 blood donors who donated at the donation sites and 500 non-donors who never donated and passed by the station were asked to complete a self-administered questionnaire during Dec 10, 2013 to Jun 25, 2014.
Main outcome measures: Blood donation or no donation.
Results: 1080 questionnaires were collected, of which 1034(95.7%) questionnaires were valid. 602(58.2%) participants were donors and 432(41.8%) were non-donors. Older people (OR: 1.46, 95% confidence interval: 1.24 to 1.72, p<0.01), males (1.33, CI: 1.02 to 1.71, p=0.03), non-college-students (1.76, CI: 1.16 to 2.56, p<0.01) and people with higher education level (1.27, CI: 1.11 to 1.45, p<0.01) were more likely to be donors. The main objective of blood donation was helping patients (n=405, 68.2%), and the main reason of not donating was being in poor health (n=138, 33.1%).
However, other motives, such as benefiting health and free check for blood type and body, and obstacles, such as failing to meet the requirements and fear, were also important. More male donors would donate again than females (80.5% vs. 68.5%, p<0.01), whereas more female donors showed uncertainty than males (25.9% vs. 16.6%, p<0.01). Usage of blood (n=182, 46.7%) was what non-donors wanted to know the most if they were to donate in the future. The majority of participants (n=730, 71.3%) considered raising the awareness of blood donation among people was one of the most effective ways of blood donation promotion. Television was considered as one of the most effective methods of blood donation promotion and recruitment, and was more acceptable to females. Younger participants preferred the internet.
Conclusion: These findings suggest that raising the awareness of blood donation is vital. Campaigns should focus on multiple aspects targeting different groups of people. Television and the internet are useful tools of blood donation promotion and recruitment.published_or_final_version
Public Health
Master
Master of Public Health
3
Adam, Douglas. "An investigation of a theoretical model of willingness to donate blood." Thesis, Edith Cowan University, Research Online, Perth, Western Australia, 1997. https://ro.ecu.edu.au/theses/899.
Full textAbstract:
The Australian Red Cross Blood Transfusion Service (ARCBTS) in Western Australian faces a major problem with periodic shortages of blood components. These shortages are expected to become more frequent and severe as demand continues to increase at a faster rate than supply. Given that only five percent of the population is registered as blood donors, clearly, the challenge for the ARCBTS is to encourage more people to become regular blood donors. The current study was undertaken to assist the ARCBTS in achieving this goal, by identifying and investigating the factors that influence people's willingness to donate blood. Based on the findings of a literature review and focus groups, a conceptual model of "willingness to donate blood" was developed. The model included personal values, knowledge about blood donation, perceived risks associated with donating blood, and attitudes towards blood donation, as antecedents to willingness to donate.
4
Harris, Maryke. "Deterrents to continued blood donation among regular blood donors." Thesis, Nelson Mandela Metropolitan University, 2017. http://hdl.handle.net/10948/15934.
Full textAbstract:
Collecting blood from repeat blood donors is cost effective and safer compared to other donor types. At the end of 2012, 84% of the SANBS donor panel were inactive or lapsed. There is a lack of research available on lapsed donors in the South African context and available research is mostly quantitative with subtle contradictions. Donors who donated blood in 2012 at fixed site donor centres in Port Elizabeth, and did not return in 2013, were studied. A descriptive analysis was done and a random sample of 78 lapsed donors were selected to participate in a face-to-face interview. Interviews were digitally recorded and transcribed. A grounded model was developed from various existing theories to seek out and conceptualise social patterns and structures of lapsed blood donors through a process of comparison. There were 10 062 donors who donated blood in 2012 and 4 923 became lapsed during 2013. Analysis of sub groups showed a higher proportion of donors who became lapsed in the following sub-categories: new donors (95%), re-joined donors (64%), black donors (63%), donors younger than 40 (61%), female donors (52%). The feedback received from the 11 participants highlighted peer pressure as the biggest motivator. Of the six communication theories applied, The Social Penetration Theory highlighted the cost-minus-benefit ratio which played a big role in a donor’s motivation and decision to return. The AIDA Marketing Model application described lapsed donor behaviour most comprehensively and it highlighted a missing step which was created as part of a Grounded Model and is called the AIDAA Model. The role and existence of peer pressure is directly linked to donor motivation and is categorised as an Action Motivator in the AIDAA Model. The new model creates additional recruitment opportunities which has not been explored and applied strategically before.
5
Prado, Eric A. "Measuring Biomarkers From Dried Blood Spots Utilizing Bead-based Multiplex Technology." Thesis, University of North Texas, 2014. https://digital.library.unt.edu/ark:/67531/metadc699876/.
Full textAbstract:
Dried blood spots is an alternative method to collect blood samples from research subjects. However, little is known about how hemoglobin and hematocrit affect bead-based multiplex assay performance. The purpose of this study was to determine how bead-based multiplex assays perform when analyzing dried blood spot samples. A series of four experiments outline the study each with a specific purpose. A total of 167 subject samples were collected and 92 different biomarkers were measured. Median fluorescence intensity results show a positive correlation between filtered and non-filtered samples. Utilizing a smaller quantity of sample results in a positive correlation to a larger sample. Removal of hemoglobin from the dried blood spot sample does not increase detection or concentration of biomarkers. Of the 92 different biomarkers measured 56 were detectable in 100-75% of the attempted samples. We conclude that blood biomarkers can be detected using bead-based multiplex assays. In addition, it is possible to utilize a smaller quantity of sample while avoiding the use of the entire sample, and maintaining a correlation to the total sample. While our method of hemoglobin was efficient it also removed the biomarkers we wished to analyze. Thus, an alternative method is necessary to determine if removing hemoglobin increases concentration of biomarkers. More research is necessary to determine if the biomarkers measured in this study can be measured over time or within an experimental model.
6
Adams, Faieqa. "An in vitro comparison of cellular destruction and metabolic effects occurring in stored, leuco-reduced and irradiated red blood cells." Thesis, Cape Peninsula University of Technology, 2016. http://hdl.handle.net/20.500.11838/2457.
Full textAbstract:
Thesis (MTech (Biomedical Technology))--Cape Peninsula University of Technology, 2016.Biochemical and haematological changes occur in red blood cellular products during the recommended storage period of 35 to 42 days at 1°C to 6°C. The restriction of the sodium/potassium pump at specified temperatures result in low intracellular potassium ion levels while an increase in sodium ion levels are observed and acidosis occurs as a result of low pH concentrations due to glucose consumption. Structural and morphological changes occur such as the release of free haemoglobin, lactate dehydrogenase and potassium into the supernatant causing the formation of spheroechinocytes and osmotic fragility. All these factors negatively impact the rheological properties of blood. These changes that transpire in the red cells during the storage period are referred to as “storage lesions”. Transfusion-associated graft versus host disease is an immunological and often fatal adverse transfusion reaction with gamma irradiation of cellular blood products used as a preventative measure. Gamma irradiation exacerbates storage lesions and of particular concern has been the increased potassium levels resulting in neonatal and infant hyperkalaemia. The storage lesions occurring in non-irradiated red blood cellular products are well documented although the literature regarding its irradiated counterparts has been less studied. A study of this nature has not previously been done in Cape Town, South Africa.
7
Garcia, Claudia Zeferino. "Estabilidade do fator de von Willebrand e fator VIII no crioprecipitado canino em diferentes protocolos de armazenamento /." Botucatu, 2014. http://hdl.handle.net/11449/124094.
Full textAbstract:
Orientador: Regina Kiomi TakahiraBanca: Luiz Henrique de Araújo Machado
Banca: Simone Gonçalves Rodrigues Gomes
Resumo: O fator VIII (FVIII), o fator de von Willebrand (FvW) e o fibrinogênio são de suma importância na coagulação sanguínea, com diferentes funções fisiológicas. Por conter altas concentrações destes fatores a transfusão de crioprecipitado é uma terapia utilizada principalmente em pacientes que apresentam Doença de von Willebrand, Hemofilia A (deficiência do FVIII), ou pacientes que sofrem de hipo ou disfibrinogenemia. Este hemocomponente é um precipitado obtido após o descongelamento parcial (entre 1 e 6°C) do plasma fresco congelado, e também é conhecido como fator anti-hemofílico. Estudos têm demonstrado que o protocolo de congelamento e armazenamento do crioprecipitado afeta a qualidade do produto e a viabilidade destes fatores. Com o objetivo de avaliar a viabilidade do crioprecipitado canino em diferentes protocolos de congelamento e armazenamento foram avaliados dois grupos compostos de 10 unidades de crioprecipitado canino (n=20). Após a centrifugação das bolsas de sangue, o plasma fresco foi congelado a -80ºC (grupo I) e a -20ºC (grupo II). Vinte e quatro horas após o congelamento das bolsas, estas foram submetidas ao procedimento de extração do crioprecipitado. Os crioprecipitados das bolsas dos dois grupos foram submetidos à determinação do TP, TTPA, FVIII, FvW e fibrinogênio, no momento zero e após seis meses de estocagem. Para a realização das coletas, foram utilizadas bolsas sanguíneas triplas de plástico, com anticoagulante CPDA-1, sendo a bolsa principal com capacidade para 450 mL de sangue total (JP Indústria Farmacêutica®). Após o crioprecipitado devidamente pronto, uma alíquota de aproximadamente 5 mL da bolsa de crioprecipitado foi separada em criotubos para análise da amostra pré-estocagem e seis meses pós estocagem. As amostras obtidas em cada momento foram congeladas à -80ºC até o momento do processamento. Os resultados mostraram um decréscimo significativo dos fatores e ...
Abstract: The factor VIII (FVIII), the von Willebrand factor (vWF) and the fibrinogen are extremely important to the blood clotting process, with various physiological functions. Because it contains high concentrations of these factors and fibrinogen, transfusing cryoprecipitate is a therapy mainly used in patients who have von Willebrand disease, Hemophilia A (FVIII deficiency), or who suffer from hypo/dysfibrinogenemia. This hemocomponent is a precipitate obtained after the partial thawing process (between 1 and 6ºC) of fresh frozen plasma, and which is also known as the anti-hemophilic factor. Studies have demonstrated that the cryoprecipitate freezing and storage protocol affects the product quality as well as these factors viability. In order to evaluate the canine cryoprecipitate viability in different freezing and storage protocols, two groups containing 10 units of canine cryoprecipitate (n=20) were evaluated. Following the blood centrifugation, the fresh plasma was frozen at -80ºC (group I) and at -20ºC (group II). Twenty-four hours after freezing the blood bags, they were submitted to the cryoprecipitate extraction procedure. The cryoprecipitate from both groups of blood bags were submitted to the TP, TTPA, FVIII, FvW and fibrinogen determination process, at time zero and after six months of storage. During the collections, triple plastic blood bags were used, along with the anticoagulant CPDA-1, being the main bag capacity of 450 mL of whole blood (JP Indústria Farmacêutica®). After having the cryoprecipitate properly ready, an approximately 5 mL aliquot of cryoprecipitate was separated into cryovials to be analysed pre-storage and six months after storage. However, there was no significant difference between treatments, demonstrating that the difference in initial freezing temperature did not influence the decrease of the factors after six months storage at -20°C
Mestre
8
Garcia, Claudia Zeferino [UNESP]. "Estabilidade do fator de von Willebrand e fator VIII no crioprecipitado canino em diferentes protocolos de armazenamento." Universidade Estadual Paulista (UNESP), 2014. http://hdl.handle.net/11449/124094.
Full textAbstract:
Made available in DSpace on 2015-06-17T19:34:48Z (GMT). No. of bitstreams: 0
Previous issue date: 2014-07-29. Added 1 bitstream(s) on 2015-06-18T12:47:02Z : No. of bitstreams: 1
000831074.pdf: 615076 bytes, checksum: c97e8862ed17121a7d6c2ddd7dfb24e1 (MD5)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
O fator VIII (FVIII), o fator de von Willebrand (FvW) e o fibrinogênio são de suma importância na coagulação sanguínea, com diferentes funções fisiológicas. Por conter altas concentrações destes fatores a transfusão de crioprecipitado é uma terapia utilizada principalmente em pacientes que apresentam Doença de von Willebrand, Hemofilia A (deficiência do FVIII), ou pacientes que sofrem de hipo ou disfibrinogenemia. Este hemocomponente é um precipitado obtido após o descongelamento parcial (entre 1 e 6°C) do plasma fresco congelado, e também é conhecido como fator anti-hemofílico. Estudos têm demonstrado que o protocolo de congelamento e armazenamento do crioprecipitado afeta a qualidade do produto e a viabilidade destes fatores. Com o objetivo de avaliar a viabilidade do crioprecipitado canino em diferentes protocolos de congelamento e armazenamento foram avaliados dois grupos compostos de 10 unidades de crioprecipitado canino (n=20). Após a centrifugação das bolsas de sangue, o plasma fresco foi congelado a -80ºC (grupo I) e a -20ºC (grupo II). Vinte e quatro horas após o congelamento das bolsas, estas foram submetidas ao procedimento de extração do crioprecipitado. Os crioprecipitados das bolsas dos dois grupos foram submetidos à determinação do TP, TTPA, FVIII, FvW e fibrinogênio, no momento zero e após seis meses de estocagem. Para a realização das coletas, foram utilizadas bolsas sanguíneas triplas de plástico, com anticoagulante CPDA-1, sendo a bolsa principal com capacidade para 450 mL de sangue total (JP Indústria Farmacêutica®). Após o crioprecipitado devidamente pronto, uma alíquota de aproximadamente 5 mL da bolsa de crioprecipitado foi separada em criotubos para análise da amostra pré-estocagem e seis meses pós estocagem. As amostras obtidas em cada momento foram congeladas à -80ºC até o momento do processamento. Os resultados mostraram um decréscimo significativo dos fatores e ...
The factor VIII (FVIII), the von Willebrand factor (vWF) and the fibrinogen are extremely important to the blood clotting process, with various physiological functions. Because it contains high concentrations of these factors and fibrinogen, transfusing cryoprecipitate is a therapy mainly used in patients who have von Willebrand disease, Hemophilia A (FVIII deficiency), or who suffer from hypo/dysfibrinogenemia. This hemocomponent is a precipitate obtained after the partial thawing process (between 1 and 6ºC) of fresh frozen plasma, and which is also known as the anti-hemophilic factor. Studies have demonstrated that the cryoprecipitate freezing and storage protocol affects the product quality as well as these factors viability. In order to evaluate the canine cryoprecipitate viability in different freezing and storage protocols, two groups containing 10 units of canine cryoprecipitate (n=20) were evaluated. Following the blood centrifugation, the fresh plasma was frozen at -80ºC (group I) and at -20ºC (group II). Twenty-four hours after freezing the blood bags, they were submitted to the cryoprecipitate extraction procedure. The cryoprecipitate from both groups of blood bags were submitted to the TP, TTPA, FVIII, FvW and fibrinogen determination process, at time zero and after six months of storage. During the collections, triple plastic blood bags were used, along with the anticoagulant CPDA-1, being the main bag capacity of 450 mL of whole blood (JP Indústria Farmacêutica®). After having the cryoprecipitate properly ready, an approximately 5 mL aliquot of cryoprecipitate was separated into cryovials to be analysed pre-storage and six months after storage. However, there was no significant difference between treatments, demonstrating that the difference in initial freezing temperature did not influence the decrease of the factors after six months storage at -20°C
FAPESP: 2012/13677-6
9
Elliott, Jennifer. "Studies on the preservation of flowers." Thesis, University of St Andrews, 2002. http://hdl.handle.net/10023/2693.
Full textAbstract:
A known method for the preservation of green foliage was adapted in order to preserve floral tissues, retaining the colour and texture, thereby providing a method suitable for the preservation of whole flowers. Initially, the effects of the existing foliage preservation process on floral tissues were studied and the resulting problems of limp sticky petals and colour loss were identified. Subsequently, with a knowledge of basic plant anatomy and of the properties of the main floral pigments, the anthocyanins, a series of experiments on petals and whole flowers were carried out in an attempt to rectify these problems and to incorporate the remedies into a method for preserving whole flowers. The problem of improving the texture and firmness of flower heads was tackled by investigating the effects of adding bulking or setting ingredients to the process fluid and establishing their optimum concentrations. In the case of flower colour, the addition of acid was required in order to maintain the bright anthocyanin colours and a range of acids was investigated. Furthermore, since it is known that in nature the anthocyanin pigments are stabilised by metal ions and copigments, the use of these agents in the preservation process was also considered. This empirical work was then validated by confirming the identity of the main pigments involved and by studying various aspects of the new preservation process. Factors examined included acid concentration, temperature, solvent composition and the addition of metal ions and copigments to solutions of petal extracts containing anthocyanin pigments. Physical changes resulting from processing, including process fluid content and the moisture absorption properties of processed petals were also measured. Finally, the application of a selection of coating materials was assessed in an attempt to increase the life span of the processed flowers by providing extra protection against environmental stresses.
10
Vernoski, Barbara K. "Effect of Blood Collection Practices on Emergency Department Blood Specimen Rejection Rates." UNF Digital Commons, 2013. http://digitalcommons.unf.edu/etd/438.
Full textAbstract:
The practice of obtaining blood as part of the placement of a new peripheral venous access device (p-VAD) is a frequent practice in the emergency department (ED). Of the concerns related to this practice is the possibility of laboratory specimen rejection due to p-VAD catheter size, use of the wrong collection device, and the absence of a standardized collection process. The objective of this study, therefore, was to examine the effect of the use of evidence-based venipuncture and p-VAD blood collection protocols on the rejection rate of blood specimens drawn by staff in the adult areas of an urban academic medical center ED.
A convenience sample of 28 ED nurses and 39 ED technicians (51.94% of all eligible ED employees) consented to using these evidence based protocols when they collected blood from adult ED patients. Blood specimen rejections rates were measured for four consecutive weeks prior to and at weeks 1-4, 5-8, 9-12, and 1-12 after the evidence-based blood collection practices training intervention. Laboratory analysis of all specimens was automated with rejection results provided in the form of computerized reports.
There was a significant decrease in the 12-week rejection rates for two of the three ED adult care areas, with the overall ED adult area rejection rate significantly decreased from 3.19% to 2.38% (X2at Df1, p < .05). The most common reasons for rejection were hemolysis (65.39%) and clotting (10.68%) followed by specimen mis-labeling, tube missing, insufficient quantity for testing, incorrect packaging, specimen contamination or dilution, and label missing, Though the use of theses evidence based blood collection protocols significantly decreased the overall rejection rate, the high percent of rejections due to hemolysis may further be reduced by having all ED staff use these protocols, and by exploring other collection techniques in the literature that have been found to significantly decrease rejection rates.
11
Dodson, Patricia W. "The Blood Bank: A Collection of Short Stories." VCU Scholars Compass, 2016. http://scholarscompass.vcu.edu/etd/4214.
Full textAbstract:
In this collection of fourteen stories, the author explores the connections that hold families together. She is particularly interested in how personal memory, episodic violence and unspoken love seem to propel generations of families in surprising ways.
12
Wlodarczyk, Tomasz. "STABILITY AND PRESERVATION PROPERTIES OF MULTISYMPLECTIC INTEGRATORS." Doctoral diss., University of Central Florida, 2007. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/3510.
Full textAbstract:
This dissertation presents results of the study on symplectic and multisymplectic numerical methods for solving linear and nonlinear Hamiltonian wave equations. The emphasis is put on the second order space and time discretizations of the linear wave, the Klein-Gordon and the sine-Gordon equations. For those equations we develop two multisymplectic (MS) integrators and compare their performance to other popular symplectic and non-symplectic numerical methods. Tools used in the linear analysis are related to the Fourier transform and consist of the dispersion relationship and the power spectrum of the numerical solution. Nonlinear analysis, in turn, is closely connected to the temporal evolution of the total energy (Hamiltonian) and can be viewed from the topological perspective as preservation of the phase space structures. Using both linear and nonlinear diagnostics we find qualitative differences between MS and non-MS methods. The first difference can be noted in simulations of the linear wave equation solved for broad spectrum Gaussian initial data. Initial wave profiles of this type immediately split into an oscillatory wave-train with the high modes traveling faster (MS schemes), or slower (non-MS methods), than the analytic group velocity. This result is confirmed by an analysis of the dispersion relationship, which also indicates improved qualitative agreement of the dispersive curves for MS methods over non-MS ones. Moreover, observations of the convergence patterns in the wave profile obtained for the sine-Gordon equation for the initial data corresponding to the double-pole soliton and the temporal evolution of the Hamiltonian functional computed for solutions obtained from different discretizations suggest a change of the geometry of the phase space. Finally, we present some theoretical considerations concerning wave action. Lagrangian formulation of linear partial differential equations (PDEs) with slowly varying solutions is capable of linking the wave action conservation law with the dispersion relationship thus suggesting the possibility to extend this connection to multisymplectic PDEs.Ph.D.
Department of Mathematics
Sciences
Mathematics PhD
13
Marques, Graça Susete Costa de Carvalho. "Establishing a cell biology platform: isolation and preservation of human blood products." Master's thesis, Faculdade de Ciências e Tecnologia, 2014. http://hdl.handle.net/10362/11009.
Full textAbstract:
Dissertação para obtenção do Grau de Mestre em
Genética Molecular e BiomedicinaThe use of human primary cells provide researchers in different areas with irrefutable more biologically relevant data than using cell lines or animal blood cells. The work was performed in the scope of the Cell Biology Services @ CEDOC, aiming to provide viable and trustful human primary cells and products. We had three main objectives: protocol optimizations for blood cell isolation, culture and cryopreservation; cost estimation and divulgation of the services. We have reviewed standard protocols and compared different strategies for blood cell isolation. The impact of those methodologies was evaluated regarding cell yield and purity, cell functional characteristics and cost. We also developed a method for serum isolation from human plasma in blood buffy coats. The resultant sera were sterile and suitable to be used in leukocyte cultures. Different protocols for T cells isolation were compared: positive versus negative immunomagnetic selection and isolation using nylon wool fiber columns. Positive selection provided the highest isolation yield (32.35%), while negatively selected cells had the highest purity (92.81%). Although nylon wool fiber column was the fastest and cheapest method, unlike the immunomagnetic methods, it did not allow complete separation of T from B lymphocytes. Positive selection of monocytes was compared using two widely used commercial kits. Miltenyi’s kit provided the highest isolation yield (25.92%), recovery rate (86.70%) and purity (95.01%). Monocytes isolated with StemCell kit presented a higher cell complexity, and when differentiated into dendritic cells (DCs), showed a more mature phenotype. Differences between both kits are probably caused by the nature of the magnetic beads, suggesting caution when choosing one or other kit, as it may have an impact on DCs’ function. Overall, although dealing with apparently straight forward methodologies, our results show that testing commercial products and optimizing protocols is very important and contribute for a better quality of products and services.
14
Cooper, Valerie Y. "The crying of the blood : a collection of short stories." Virtual Press, 2006. http://liblink.bsu.edu/uhtbin/catkey/1337191.
Full textAbstract:
The Crying of the Blood is a collection of short stories with the two characters Mariah and Mary, born one hundred years apart, who deal with the challenges of life dealt them. Through descriptive language and the strong presence of place and setting, the author explores the under-girding strength of human nature in dealing with the external and internal pressures of the various forms of war and its aftermath. By examining the effects of the human condition through inherited and acquired traits passed to succeeding descendents of the characters, the author exposes the foibles of human nature. People live a specific way and repeat patterns of thinking and choosing without knowing why or stopping to consider the ensuing results of their actions. The collection of stories reveals the dark shadows of the Civil War that continue to shape the Southern culture and also the enduring strength and charm of the people and their traditions.This collection of stories is a work of fiction. Names, characters, places, and incidents are either a figment of the author's imagination or used in a fictitious manner. Resemblances to actual people, settings, and events are purely coincidental.Department of English
15
Zeng, Chenxi. "A minimum cost and risk mitigation approach for blood collection." Diss., Georgia Institute of Technology, 2016. http://hdl.handle.net/1853/54966.
Full textAbstract:
Due to the limited supply and perishable nature of blood products, effective management of blood collection is critical for high quality healthcare delivery. Whole blood is typically collected over a 6 to 8 hour collection window from volunteer donors at sites, e.g., schools, universities, churches, companies, that are a significant distance
from the blood products processing facility and then transported from collection site to processing facility by a blood mobile. The length of time between collecting whole blood and processing it into cryoprecipitate ("cryo"), a critical blood product for controlling massive hemorrhaging, cannot take longer than 8 hours (the 8 hour collection to completion constraint), while the collection to completion constraint for other blood products is 24 hours. In order to meet the collection to completion constraint for cryo, it is often necessary
to have a "mid-drive collection"; i.e., for a vehicle other than the blood mobile to pickup and transport, at extra cost, whole blood units collected during early in the collection window to the processing facility. In this dissertation, we develop analytical models to: (1)
analyze which collection sites should be designated as cryo collection sites to minimize total collection costs while satisfying the collection to completion constraint and meeting the weekly production target (the non-split case), (2) analyze the impact of changing the current process to allow collection windows to be split into two intervals and then determining which intervals should be designated as cryo collection intervals (the split case), (3) insure that the weekly production target is met with high probability. These problems lead to MDP models with large state and action spaces and constraints to guarantee that the weekly production target is met with high probability. These models are computationally intractable for problems having state and action spaces of realistic cardinality. We consider two approaches to guarantee that the weekly production target is met with high probability: (1) a penalty function approach and (2) a chance constraint approach. For the MDP with penalty function approach, we first relax a constraint that significantly reduces the cardinality of the state space and provides a lower bound on the optimal expected weekly cost of collecting whole blood for cryo while satisfying the collection to completion constraint. We then present an action elimination procedure that coupled with the constraint relaxation leads to a computationally tractable lower bound. We then develop several heuristics that generate sub-optimal policies and provide an analytical description of the difference between the upper and lower bounds in order to determine the quality of the heuristics. For the multiple decision epoch MDP model with chance constraint approach, we first note by example that a straightforward application of dynamic programming can lead to a sub-optimal policy. We then restrict the model to a single decision epoch. We then use a computationally tractable rolling horizon procedure for policy determination. We also present a simple greedy heuristic (another rolling horizon decision
making procedure) based on ranking the collection intervals by mid-drive pickup cost per unit of expected cryo collected, which results in a competitive sub-optimal solution and leads to the development of a practical decision support tool (DST). Using real data from the American Red Cross (ARC), we estimate that this DST reduces total cost by about 30% for the non-split case and 70% for the split case, compared to the current practice. Initial implementation of the DST at the ARC Southern regional manufacturing and service center supports our estimates and indicates the potential for significant improvement in current practice.
16
Dougherty, Meghan. "Archiving the Web : collection, documentation, display, and shifting knowledge production paradigms /." Thesis, Connect to this title online; UW restricted, 2007. http://hdl.handle.net/1773/6153.
Full text
17
Suontaka, Anna-Maija. "Haemostatic changes in plasma for transfusion during preparation and storage /." Stockholm, 2006. http://diss.kib.ki.se/2006/91-7140-449-X/.
Full text
18
Skornicka, Erin L. "The effect of blood collection methods on the expression of monocyte cellular adhesion molecules /." Connect to online version, 1996. http://hdl.handle.net/1989/3570.
Full text
19
Lienhoop, Nele. "Valuing wilderness preservation in Iceland using WTP and WTA : an investigation into data collection modes." Thesis, University of Aberdeen, 2004. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.415482.
Full textAbstract:
This study reports an application of the Contingent Valuation method (CV) to estimate the non-market costs and benefits of hydro scheme developments in an Icelandic wilderness area. A deliberative group-based approach, called Market Stall (MS), is compared to a control group consisting of conventional in-person interviews, in order to investigate flaws of CV, such as poor validity, high non-response rates to the elicitation question and protest responses. A careful exploration into perceived property rights led to the use of willingness to accept (WTA) in compensation for wilderness loss and willingness to pay (WTP) for hydro scheme related benefits. Furthermore, a novel approach to participant behaviour observation and debriefing questions provided insights into the shortcomings of conventional data collection modes, in terms of encouraging motivation, standardised information sets and time pressure, as well as a useful supplement to validity testing. The study suggests that public preferences for the preservation of the wilderness area (status quo) outweigh preferences for non-market hydro scheme benefits. Mean WTA accounts for 780.107 kr (MS estimates) and 86,328 kr (control group estimates), while mean WTP totals 21,326 kr (MS estimates) and 6,377 kr (control estimates). According to this study, MS is better suited for preference construction over complex environmental goods: estimates have more explanatory power, the non-response rate to the elicitation question is lower, and respondents are more engaged. A further finding provides evidence that individuals have differing information requirements, and hence standardised information sets may not be suitable in CV. However, according to the debriefing questions, MS adjusted information to individual needs better than interviews. Although previous research has avoided using WTA due to high mental demands, protesting and strategic bidding, the questionnaire design used in this study resulted in both MS and interview respondents reporting valid WTA bids.
20
Hudson, Davia Tamar. "Variables Affecting the Collection and Preservation of Human Scent Components through Instrumental and Biological Evaluations." FIU Digital Commons, 2009. http://digitalcommons.fiu.edu/etd/201.
Full textAbstract:
In certain European countries and the United States of America, canines have been successfully used in human scent identification. There is however, limited scientific knowledge on the composition of human scent and the detection mechanism that produces an alert from canines. This lack of information has resulted in successful legal challenges to human scent evidence in the courts of law. The main objective of this research was to utilize science to validate the current practices of using human scent evidence in criminal cases. The goals of this study were to utilize Headspace Solid Phase Micro Extraction Gas Chromatography Mass Spectrometry (HS-SPME-GC/MS) to determine the optimum collection and storage conditions for human scent samples, to investigate whether the amount of DNA deposited upon contact with an object affects the alerts produced by human scent identification canines, and to create a prototype pseudo human scent which could be used for training purposes. Hand odor samples which were collected on different sorbent materials and exposed to various environmental conditions showed that human scent samples should be stored without prolonged exposure to UVA/UVB light to allow minimal changes to the overall scent profile. Various methods of collecting human scent from objects were also investigated and it was determined that passive collection methods yields ten times more VOCs by mass than active collection methods. Through the use of polymerase chain reaction (PCR) no correlation was found between the amount of DNA that was deposited upon contact with an object and the alerts that were produced by human scent identification canines. Preliminary studies conducted to create a prototype pseudo human scent showed that it is possible to produce fractions of a human scent sample which can be presented to the canines to determine whether specific fractions or the entire sample is needed to produce alerts by the human scent identification canines.
21
Breit, Elyse. "Education for Pediatric Oncology Nurses on Fertility Preservation of Pediatric Oncology Patients." Honors in the Major Thesis, University of Central Florida, 2014. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1578.
Full textAbstract:
Although the survival rate of childhood cancer is high, nearly two thirds of these survivors experience negative long-term secondary side effects from cancer treatments. Infertility is one such side effect that can have a prominent impact on quality of life as the patient ages. It is important for nurses working with pediatric oncology patients to provide the patient and family with education about risk for infertility and fertility preservation (FP) in order to allow families to make decisions about FP before cancer treatment starts. However, pediatric
oncology nurses report being uneducated about FP guidelines and are hesitant to broach this subject with families. The purpose of this HIM thesis is to review nurse perceived barriers
related to educating patients and their families about the risk for infertility following cancer treatments and FP and to make recommendations for improving communication between nurses and families about FP. A search was performed using CINAHL, PreCINAHL, PsychINFO, PsychARTICLES, and Medline databases and examined peer-reviewed quantitative and
qualitative research studies. Key terms used in the database searches were ped' OR child', onco' OR cancer', fert', and nurs'. Findings indicated that there were many barriers for
pediatric oncology nurses, which inhibited the discussion of FP with patients and families such as lack of knowledge and resources, provider attitudes toward FP, and patient factors. Based on the findings, the researcher identified several interventions to aid pediatric oncology nurses in overcoming these barriers to FP discussion.B.S.N.
Bachelors
Nursing
Nursing
22
Wallin, Olof. "Preanalytical errors in hospitals : implications for quality improvement of blood sample collection." Doctoral thesis, Umeå universitet, Institutionen för medicinsk biovetenskap, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-1672.
Full textAbstract:
Background: Most errors in the venous blood testing process are preanalytical, i.e. they occur before the sample reaches the laboratory. Unlike the laboratory analysis, the preanalytical phase involves several error-prone manual tasks not easily avoided with technological solutions. Despite the importance of the preanalytical phase for a correct test result, little is known about how blood samples are collected in hospitals. Aim: The aim of this thesis was to survey preanalytical procedures in hospitals to identify sources of error. Methods: The first part of this thesis was a questionnaire survey. After a pilot study (Paper I), a questionnaire addressing clinical chemistry testing was completed by venous blood sampling staff (n=314, response rate 94%) in hospital wards and hospital laboratories (Papers II–IV). The second part of this thesis was an experimental study. Haematology, coagulation, platelet function and global coagulation parameters were compared between pneumatic tube-transported samples and samples that had not been transported (Paper V). Results: The results of the questionnaire survey indicate that the desirable procedure for the collection and handling of venous blood samples were not always followed in the wards (Papers II–III). For example, as few as 2.4% of the ward staff reported to always label the test tube immediately before sample collection. Only 22% of the ward staff reported to always use wristbands for patient identification, while 18% reported to always use online laboratory manuals, the only source of updated information. However, a substantial part of the ward staff showed considerable interest in re-education (45%) and willingness to improve routines (44%) for venous blood sampling. Compared to the ward staff, the laboratory staff reported significantly higher proportions of desirable practices regarding test request management, test tube labelling, test information search procedures, and the collection and handling of venous blood samples, but not regarding patient identification. Of the ward staff, only 5.5% had ever filed an error report regarding venous blood sampling, compared to 28% of the laboratory staff (Paper IV). In the experimental study (Paper V), no significant preanalytical effect of pneumatic tube transport was found for most haematology, coagulation and platelet function parameters. However, time-to-clot formation was significantly shorter (16%) in the pneumatic tube-transported samples, indicating an in vitro activation of global coagulation. Conclusions. The questionnaire study of the rated experiences of venous blood sampling ward staff is the first of its kind to survey manual tasks in the preanalytical phase. The results suggest a clinically important risk of preanalytical errors in the surveyed wards. Computerised test request management will eliminate some, but not all, of the identified risks. The better performance reported by the laboratory staff may reflect successful quality improvement initiatives in the laboratories. The current error reporting system needs to be functionally implemented. The experimental study indicates that pneumatic tube transport does not introduce preanalytical errors for regular tests, but manual transport is recommended for analysis with thromboelastographic technique. This thesis underscores the importance of quality improvement in the preanalytical phase of venous blood testing in hospitals.
23
Alfonso, Lizarazo Edgar. "Optimization of blood collection systems : Balancing service quality given to the donor and the efficiency in the collection planning." Thesis, Saint-Etienne, EMSE, 2013. http://www.theses.fr/2013EMSE0698/document.
Full textAbstract:
Les rapports d’activité de l’Établissement Français du Sang (EFS) font état d’une demande croissante de produits sanguins labiles (PSL) tels les concentrés globules rouges (CGR), les plaquettes, et le plasma. Afin d’assurer la demande vitale en PSL, il est primordial d’optimiser la logistique liée aux activités de collecte du sang et de ses composants. Pour faire face à cette situation, l’EFS Auvergne-Loire mène une réflexion dans le but d’utiliser de manière plus efficiente les dispositifs de collecte en sites fixes et mobiles pour améliorer (i) la qualité de service rendue au donneur, et (ii) l’efficience de l’utilisation des ressources humaines. Dans ce contexte nous avons développé dans cette thèse des outils opérationnels pour (i) la modélisation des dispositifs de collecte, (ii) la régulation des flux de donneurs, et (iii) la planification de collectes mobiles.La méthode d'analyse des dispositifs de collecte est basée sur des techniques de simulation à événements discrets. Une modélisation préalable des flux de donneurs dans les systèmes de collecte en sites fixes et mobiles à l’aide de réseaux de Petri a été proposée. Pour la régulation de flux de donneurs, notamment pour la planification optimale des rendez-vous des donneurs et la planification de la capacité dans les systèmes de collecte au site fixe, deux approches ont été abordées: (a) Construction d'un algorithme basée sur techniques d'optimisation stochastique via simulation ; (b) Programmation mathématique: Modèle de programmation en nombres entiers non-linéaire (MINLP) basée sur réseaux de files d'attente et représentation et évaluation des systèmes à événements discrets à travers de programmation mathématique. Pour la planification de collectes mobiles. Deux types de modèles ont été développés : (a) Au niveau tactique : Modèles de programmation en nombres entiers linéaire (MIP) pour planifier les semaines de collectes pour chaque ensemble disponible sur un horizon de temps pour garantir l'autosuffisance à niveau régional des CGR. (b) Au niveau opérationnel : Modèle de programmation en nombres entiers linéaire (MIP) pour l’organisation du travail des équipes en charge de la collecteActivity reports of the French Blood Establishment (EFS) indicate a growing demand for Labile Blood Products (LBP) as red blood cells (RBC), platelets and plasma. To ensure the vital demand of labile blood products (LBP), it’s essential to optimize the logistics related with the collection of blood components. To deal with this situation, the EFS Auvergne-Loire carry out a reflection in order to use more efficiently the collection devices in fixed and mobile sites, to improve the quality of service offered to the donor and the efficiency of human resources. In this context we have developed in this thesis operational tools for (i) modeling of blood collection devices (ii) The regulation of flows donors (iii) Planning of bloodmobile collections.The method analysis of collection devices is based on techniques of discrete event simulation. A preliminary modeling of donors’ flow in fixed and mobile collection systems using Petri nets was conducted. For the regulation of flow of donors, i.e. the optimal capacity planning and appointment scheduling of blood collections, two approaches were considered: (a) Simulation based-optimization.(b) Mathematical Programming: Mixed integer nonlinear programming (MINLP) based on queuing networks and mathematical programming representation of discrete event systems. For planning of bloodmobile collections. Two models have been developed: (a) At the tactical level: Mixed integer linear programming (MIP) to determine the weeks in which the mobile collection must be organized in order to ensure the regional self-sufficiency of RBC. (b) At the operational level: Mixed integer linear programming (MIP) for the planning of human resources in charge of blood collections
24
Ledent-Semple, Elisabeth. "A study of factors influencing the quality of blood products during preparation, storage and filtration /." Linköping : Univ, 2001. http://www.bibl.liu.se/liupubl/disp/disp2001/med667s.pdf.
Full text
25
Baverstock, P. R. "Studies in the adaptation and evolution of the Australasian fauna : a collection /." Title page, introduction and index only, 1987. http://web4.library.adelaide.edu.au/theses/09SD/09sdb353.pdf.
Full textAbstract:
Thesis (D. Sc.)--University of Adelaide, 1988.Collection of previously published articles. Includes Allozyme electrophoresis / B.J. Richardson, P.R. Baverstock and M. Adams (1986). Includes bibliographical references.
26
Nilsson, Karin. "Adherence to Venous Blood Specimen Collection Practice Guidelines Among Nursing Students and Healthcare Staff." Doctoral thesis, Umeå universitet, Institutionen för omvårdnad, 2016. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-120082.
Full textAbstract:
Background Patient safety is an undisputable part of healthcare. The use of clinical practice guidelines, usually based on evidence-based practice/best practice, promotes patient safety and high quality care, reduces unnecessary patient suffering, and healthcare costs. Analysing results from venous blood specimen collection is one of the most commonly used services within healthcare, and a substantial number of decisions on diagnosis, treatment, and treatment evaluation are based on the results. Hence, the accuracy of these tests are vitally important. Earlier research has demonstrated that healthcare staff report suboptimal adherence to venous blood specimen collection guidelines together with the need for improved practices. Blood sample collection is carried out by several professionals, among them registered nurses and, as a consequence, nursing students too. University nursing students learn and practice venous blood specimen collection in one of their first semesters. After initial skill training at clinical skill laboratories, they continue to perform the task during clinical placements in various clinical settings. Few or no studies have been performed on nursing students, hence it seemed important to assess guideline adherence to venous blood specimen collection among university students as well as to further explore adherence to guidelines among healthcare staff. Therefore, the overall aim for this thesis was to explore adherence to, and factors influencing venous blood specimen collection guidelines practice among university nursing students and healthcare staff. Methods The thesis includes four studies. Study I-III had a quantitative, cross-sectional design, study IV had a qualitative approach. Study I included 164 healthcare staff from 25 primary healthcare centres. Study II included 101 nursing students in their 5th and 6th semesters, and study III included 305 nursing students in their 2nd, 4th, and 6th semesters. To assess adherence to venous blood specimen collection guidelines, data were collected using the Venous Blood Specimen Questionnaire, completed with background variables (I, II, III) and additional scales (III). Descriptive statistics, multilevel and multiple logistic regression analyses were used to analyse the data. In study IV, data were collected through five focus group interviews among 6th semester nursing students (n=26). Data were analysed using qualitative content analysis. Results Workplace affiliation was found to explain variances in reported adherence between different primary healthcare centres. Associations between reported venous blood specimen collection practices and individual as well as workplace factors were revealed. Nursing students were found to increasingly deviate from guideline adherence during their education. Also among students, several associations between guideline adherence and other iv factors were revealed. Reported research use at clinical practice was associated with higher levels of adherence, as were higher capability beliefs regarding both evidence-based practice and academic ability. Analyses from focus group interviews summarised students’ reflections on deviations from VBSC guidelines in the overall theme ‘Striving to blend in and simultaneously follow guidelines’. Conclusion Both healthcare staff at primary healthcare centres and nursing students demonstrate decreasing levels of guideline adherence with time. Factors influencing adherence are both individual as well as contextual. This indicate that both students and staff are subjected to socialisation processes that influences levels of adherence. In order to enhance venous blood specimen collection practices and thereby patient safety, actions must be taken - both in healthcare clinical contexts and by educators. The use of models in practical skill training, and in the ambition to bridge the theory-practice gap may be the path to success. It is reasonable to assume that collaboration between, on the one hand, education representatives and on the other, supervising RNs in clinical settings, will be fruitful. Finally, by empowering students their self-efficacy may be strengthened, and hence their ability to maintain guideline adherence.
27
GIORGI, G. BAGNAGATTI DE. "PRELIMINARY EVALUATION OF THE QUALITY OF BLOOD COMPONENTS FOR TRANSFUSION USE (WHOLE BLOOD, PACKED RED BLOOD CELLS, FRESH FROZEN PLASMA) IN CANINE, FELINE AND BOVINE BLOOD PRODUCTS, AND PREPARATION OF BLOOD COMPONENT FOR NON-TRANSFUSION USE (PLATELET RICH PLASMA)IN DOG." Doctoral thesis, Università degli Studi di Milano, 2013. http://hdl.handle.net/2434/219125.
Full textAbstract:
Evaluation of hematological parameters, ammonia concentration and microbial contamination in Canine Packed Red Blood Cells stored in CPD-SAGM for 42 days.
Abstract
Canine transfusion medicine practices have been growing rapidly over the past few decades and the use of specific blood components (packed red cells, plasma) has permitted to optimized the canine blood donations. The study was undertaken to evaluated the changes in RBC, MCV, Ht, RDW, WBC and ammonia concentration in canine PRBC stored in CPD-SAGM for 42 days. Also the presence of bacterial contamination was evaluated with blood culture. PRBC units were stored in a routine manner and were examined every 2-3 weeks. Hematological parameters changed significantly with increase of MCV, Ht and RDW, while WBC decreased. Also ammonia concentration increased significantly during the storage. RBC and WBC deteriorated somewhat during storage and ammonia concentration increased similar to what reported in canine and human in vitro studies. No bacterial contamination was reported. The results obtained in this study agree mostly with what previously is reported in canine and human medicine. Further studies are needed to better evaluated how the reported alterations influence viability of blood cells in canine PRBC.
The safety and quality of feline whole blood units collected with an open system and the effect of storage on hematological parameters and ammonia concentration
Abstract
The veterinary transfusion medicine is constantly in progress but still now feline blood donation, collection, and conservation of whole blood and blood products present some problems. Feline whole blood collected with open system and stored in CPDA1 for 35 days at 4°C was evaluated for hematological parameters, ammonia concentration and sterility during the storage period. Statistical analysis resulted in significant increase in ammonia concentration and decrease of WBC. No other significant changes resulted in hematological parameters (RBC, Ht, MCV, RDW). No units presented bacterial contamination during storage. The use a standardized protocol during blood collection, preparation and storage of feline whole blood permit to obtain a product without microbial contamination, minimum changes in haematological parameters but with a very high ammonia concentration.
Preliminary Evaluation on the Stability of Protein in Bovine Fresh Frozen Plasma
Abstract
The aim of this study were to evaluating preliminary stability of glucose, urea, total protein and protein fractions in bovine fresh frozen plasma (FFP). Blood was collected into human sterile double-pack blood collection system containing citrate-phosphate-dextrose-adenine (CPDA) and after centrifugation the plasma units were stored within 8 hours from blood collection at – 19°C obtaining FFP. The analysis of biochemical parameters were performed on fresh plasma after centrifugation of whole blood unit and on thawed FFP after 1 and 6 months of storage. Pre and post storage results were compared using a one way repeated measures ANOVA. Seven FFP were obtained from 7 different whole blood units. There was no significant changes in the concentrations of glucose, urea, total protein and protein fraction during the entire period of storage. This preliminary study showed that during 6 months of storage no significant changes were appeared in the evaluate biochemical parameters in bovine FFP.
Effectiveness of manual double centrifugation method for preparation of Canine Platelet-Rich Plasma
Abstract
The platelet-rich plasma (PRP) is a product derived from whole blood with a platelet concentration higher than normal range in a small amount of plasma. Regenerative capacities of PRP deriving from platelet growth factors. For this reason PRP is used in human and veterinary medicine for its capacity to stimulate cell proliferation, angiogenesis, wound healing, production of fibroblasts, collagen, osteoblasts and to accelerate the healing process. In veterinary medicine the methods use to produce PRP are not standardized and extremely numerous. The aim of this study was to describe and evaluate a manual double centrifugation method to produce canine PRP. 28 blood samples (5-10 ml with 1 ml of sodium citrate) from 28 healthy dogs were analyzed. The first centrifugation (2500 rpm for 10’) resulted in two components, blood cell component in the bottom and serum component (SEC) in the upper fraction of the tube. All SEC, the buffy coat and the first 2 mm of red blood cell was submitted to a second centrifugation (4000 rpm for 15’) and resulted in two components, platelet poor plasma (PPP) and platelet pellet in the bottom. The amount of PPP used to resuspend platelet pellet was calculated considering that all platelets previously present in the SEC was in platelet pellet. Then 50% of the calculated PPP was used to resuspend platelet pellet with the aim to obtain the final platelet concentration of about 1 million /µl.
The method proposed in this study permitted in 14/28 samples of PRP (50%) to reach the human target of 1 milion platelets/µl ± 20% and/or the target of three to six fold platelet concentration in whole blood. According to a part of human literature the concentration of platelet in PRP does not seem to be necessarily linked to its effectiveness. For this reason to complete the evaluation of the proposed method will be necessary assess the platelet viability and after apply the PRP in vivo.
28
Bölenius, Karin. "Improving venous blood specimen collection practices : method development and evaluation of an educational intervention program." Doctoral thesis, Umeå universitet, Institutionen för omvårdnad, 2014. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-87015.
Full textAbstract:
Background: About 60%–80% of decisions regarding diagnosis and treatment are based on laboratory test results. Low adherence to venous blood specimen collection (VBSC) guidelines may lead to erroneous or delayed test results, causing patient harm and high healthcare costs. Educational intervention programs (EIPs) to update, improve and sustain VBSC practices are seldom evaluated. After testing a self-reported venous blood sampling questionnaire, the overall aim of this thesis was to evaluate the impact of a large-scale EIP on healthcare personnel’s VBSC practices. Methods: The study settings were primary healthcare centres (PHCs) in northern Sweden. Participants were VBSC personnel. Data consisted of a VBSC questionnaire of self-reported practices, records of low-level haemolysis index in serum samples (specimen quality indicator), and interviews reflecting VBSC practices. First, experts on questionnaires and VBSC were consulted, and test-retest statistics were used when testing the VBSC questionnaire for validity and reliability. Thereafter, we evaluated the impact of a short, large-scale EIP with a before-after approach comparing self-reported VBSC questionnaire of two county councils. The personnel of the county councils (n = 61 PHCs) were divided into an intervention group (n = 84) and a corresponding control group (n = 79). In order to test changes in blood specimen quality we monitored haemolysis in serum samples (2008, n = 6652 samples and 2010, n = 6121 samples) from 11 PHCs. Finally, 30 VBSC personnel from 10 PHCs reported their experiences. The interview questions were open-ended with reflective elements and the interviews were analysed by qualitative content analysis. Results: The VBSC questionnaire was found to be valid and could be used to identify risk of errors (near misses) and evaluate the impact of an EIP emphasising VBSC guideline adherence. The intervention group demonstrated several significant improvements in self-reported practices after the EIP, such as information search, patient rest, test request management, patient identification, release of venous stasis, and test tube labelling. The control group showed no significant improvements. In total, PHCs showed minor differences in blood specimen quality. Interviews summarized VBSC personnel experiences in the overall theme: education opened up opportunities for reflection about safety. Conclusion: This thesis is, to our knowledge, the first to evaluate the impacts of a large-scale EIP on VBSC practices. The VBSC questionnaire and monitoring for low-level haemolysis reflected VBSC practices. The frequently occurring near-miss markers made it possible to compare and benchmark VBSC practices down to the healthcare unit and hospital ward. The short, general EIP opened up opportunities for reflection about safety and improved VBSC practices in PHCs with larger deviations from guidelines. EIPs that provide time for reflection and discussion could improve VBSC further. Directed EIPs focused on specific VBSC flaws might be more effective for some near misses in VBSC practices, while some near misses must be changed at a different level in the system. Clinical relevance: Our results indicate that monitoring and counteracting the near misses in VBSC practices is a well-functioning preventive action. We propose that the VBSC monitoring instruments (VBSC questionnaire & haemolysis index) we used and the EIP strategy proposed should be tested in additional countries with different healthcare settings. It is suggested that a national program intended to identify near misses and prevent VBSC errors be developed in the healthcare system. General e-learning programs may be cheaper than, and as effective as, the EIP program and may be performed everywhere and any time. Systematic planning, useful for reflection and with focus on the specific elements in a skill, together with VBSC guidelines, could probably increase improvements. Our studies have led to deeper and extended knowledge of the impact of an EIP on VBSC practices. Our results can be used when considering future VBSC practice interventions. Using a model for practical skills in nursing to describe VBSC in a more holistic and less technical way might highlight VBSC as a practical nursing skill.Bakgrund: Av kliniska beslut angående diagnostik och behandling baseras 60%–80% på laboratorieresultat. Därför är det helt nödvändigt att laboratorieresultat är tillförlitliga. Låg följsamhet till provtagnings anvisningar kan leda till felaktiga och fördröjda analysresultat, förorsaka skada och lidande för patienter och utgöra en stor kostnad för hälso- och sjukvården. Felaktiga provsvar beror till stor del på felaktig provtagning och provhantering och går oftast att undvika. Interventioner som avser att uppdatera och säkra korrekt venprovtagning kan leda till förbättringar men genomförda interventioner har sällan utvärderats. Efter att en enkät för självrapporterad venprovtagning testats för validitet och reliabilitet genomfördes ett omfattande interventionsprogram som utvärderades med hjälp av den testade enkäten och andra utvärderingsmått. Det övergripande syftet var att utvärdera i vilken utsträckning interventionsprogrammet påverkade provtagande personals praktiska utförande av venprovtagning. Metoder: Studierna i denna avhandling omfattade provtagande personal vid hälsocentraler i norra Sverige. För datainsamling användes en enkät som mäter självrapporterad venprovtagning, förekomst av låggradig hemolys (indikator på blodprovets kvalitet) och intervjuer. Initialt testades enkätens förmåga att mäta vad som avsetts (validitet) och testades enkätens förmåga att vid upprepade mätningar vara tillräckligt stabil (reliabilitet) för att användas i interventionsstudier. Därefter utvärderades ett kort men storskaligt interventionsprogram i preanalys inkluderande venprovtagning med före och efter mätningar. Vi jämförde provtagande personal från två landsting vid 61 hälsocentraler. Landstingens personal delades upp i en interventionsgrupp (n=84) och en motsvarande kontrollgrupp (n = 79). För att mäta kvaliteten av blodproverna extraherades uppgifter om hemolys i serumprover (2008, n = 6652 blodprov) och (2010, n = 6121 blodprov) från elva hälsocentraler i ett landsting. Slutligen, intervjuades 30 provtagande personal från 10 hälsocentraler efter att de deltagit i interventionsprogrammet. Intervjuerna var öppna och genererade korta berättelser och analyserades med innehållsanalys. Resultat: Venprovtagningsenkäten befanns vara valid och kan användas för att utvärdera personalens följsamhet till provtagningsanvisningar i venprovtagning och identifiera riskhändelser. Interventionsgruppen visade flera signifikanta förbättringar i självrapporterat utförande av venprovtagning såsom förbättrad informationssökning, vila inför provtagning, remissförfarande, kontroll av patientidentitet, användning av stas och etikettering av provrör. Kontrollgrupen visade inga signifikanta förbättringar. Blodprovskvaliteten visade små skillnader. Provtagande personals erfarenheter från intervjuerna sammanfattades i ett övergripande tema; utbildningsinsatsen öppnade upp möjligheter för reflektion om säkerhet. Slutsats: Avhandlingen är så vitt vi vet den första att utvärdera effekten av ett storskaligt interventionsprogram med hjälp av självrapporterat utförande av venprovtagning och blodprovers kvalitet (låggradig hemolys). Med dessa metoder ökar andelen riskhändelser så att jämförelser kunde göras även på enhetsnivå och avdelningsnivå. Utbildningsprogrammet öppnade upp för reflektioner om säkerhet och förbättrade utförande av venprovtagning vid enheter med större brister. Utbildningsprogram som öppnar upp för reflektion och diskussion kan leda till ökad patientsäkerhet i hälso- och sjukvården. Trots utfallet av resultaten, är riktade utbildningsinsatser för sjukvårdsenheter som uppvisar specifika brister troligtvis mer effektiva än breda utbildningsinsatser. Klinisk betydelse: Interventionsprogram avseende preanalys och venös provtagning förbättrade personalens praktiska utförande. Monitorering av och åtgärder för att minska riskhändelser är väl fungerande preventiva åtgärder. Instrumenten (självrapporterande enkät och hemolys) bör också testas i andra kontexter inom hälso- och sjukvården. Ett externt nationellt program för att identifiera och förebygga riskhändelser bör utvecklas i hälso- och sjukvården. Interventioner i form av e-lärande kan då vara ett alternativ som är billigt och effektivt. Dessutom kan systematisk planering och genomförande med fokus på reflektion av specifika delar i en färdighet vara effektivt för att uppnå förbättringar. Våra studier har bidragit till en djupare och utökad kunskap om effekten av ett interventionsprogram på utförande av venprovtagning. Resultaten kan användas vid framtida planering av utbildningsinsatser. Modeller för praktiskt färdighetsutövande inom omvårdnad kan beskriva venprovtagning ur ett helhetsperspektiv och synliggöra venprovtagning som en viktig praktisk färdighet inom omvårdnad.
Preanalys
29
Boltshauser, Rasmus. "Development of a Novel Device for Optimal Sample Blood Volume Collection from Patients with Sepsis." Thesis, KTH, Medicinteknik och hälsosystem, 2020. http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-279133.
Full textAbstract:
When performing sepsis diagnosis, the most important preanalytical variable is blood volume. Too little blood increases the risk for false negatives whereas overfilling causes increased risk for false positives. Even though this fact is known, there are case studies showing that in a majority of tests, the taken blood sample volume is not the recommended amount. As previously tried methods have been limited in their ability to tackle the problem this study aimed at creating a technical device to aid healthcare providers with blood volume sample collection. As a base, the double diamond approach by the Design Council was used. This design approach splits up the design process in four distinctly different phases (discover, define, develop, and deliver) all using their own methods to aid the creative process. After completing the discover and define phase it was determined that a non-contact capacitance liquid level sensor could operate as an ideal blood volume sample device. During the development and delivery phase prototypes were created and evaluated. The final results of this work could not give conclusive evidence concerning if a non-contact liquid level sensor could operate as an ideal blood volume collection device. The methodological approach used in this thesis can be used as inspiration for a designer to create a device for a similar or different purpose. Moreover, information from this thesis can also work as reference material to develop a device to perform ideal blood volume sample collection. Such a device would have the potential to be an essential part of the everyday workflow in sample collection from patients with sepsis worldwide and would aid in ensures effective and fast diagnostics.
30
Thakkar, Paresh. "THE REMOVAL OF MOTION ARTIFACTS FROM NON-INVASIVE BLOOD PRESSURE MEASUREMENTS." Master's thesis, University of Central Florida, 2004. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2242.
Full textAbstract:
Modern Automatic Blood Pressure Measurement Techniques are based on measuring the cuff pressure and on sensing the pulsatile amplitude variations. These measurements are very sensitive to motion of the patient or the surroundings where the patient is. The slightest unexpected movements could offset the readings of the automatic Blood Pressure meter by a large amount or render the readings totally meaningless. Every effort must be taken to avoid subjecting the body of the patient or the patient's surroundings to motion for obtaining a reliable reading. But there are situations in which we need Blood Pressure Measurements with the patient or his surroundings in motion; for instance in an ambulance while a patient is being transported to a hospital. In this thesis, we present a technique to reduce the effect of motion artifact from Blood Pressure measurements. We digitize the blood pressure waveform and use Digital Signal Processing Techniques to process the corrupted waveform. We use the differences in frequency spectra of the Blood Pressure signal and motion artifact noise to remove the motion artifact noise. The motion artifact noise spectrum is not very well defined, since it may consist of many different frequency components depending on the kind of motion. The Blood Pressure signal is more or less a periodic signal. That translates to periodicity in the frequency domain. Hence, we designed a digital filter that could take advantage of the periodic nature of the Blood Pressure Signal waveform. The filter is shaped like a comb with periodic peaks around the signal frequency components. Further processing of the filtered signal: baseline restoration and level shifting help us to further reduce the noise corruption.M.S.
Department of Electrical and Computer Engineering
Engineering and Computer Science
Electrical Engineering
31
Gentile, Russell. "Adding cerebral autoregulation to a lumped parameter model of blood flow." Honors in the Major Thesis, University of Central Florida, 2012. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/555.
Full textAbstract:
A mathematical model of blood flow in infants with hypoplastic left heart syndrome (HLHS) was improved by adding cerebral autoregulation. This is the process by which blood vessels constrict or dilate to keep blood flow steady in certain organs during pressure changes. The original lumped parameter model transformed the fluid flow into an electrical circuit. Its behavior is described using a system of thirty-three coupled differential equations that are solved numerically using a fourth-order Runge-Kutta method implemented in MATLAB. A literature review that includes a discussion of autoregulation mechanisms and approaches to modeling them is followed by a description of the model created for this paper. The model is based on the baroreceptor or neurogenic theory of autoregulation. According to this theory, nerves in certain places within the cardiovascular system detect changes in blood pressure. The brain then compensates by sending a signal to blood vessels to constrict or dilate. The model of the control system responded fairly well to a pressure drop with a steady state error of about two percent. Running the model with or without the control system activated had little effect on other parameters, notably cardiac output. A more complete model of blood flow control would include autonomic regulation. This would vary more parameters than local autoregulation, including heart rate and contractility. This is suggested as a topic of further research.B.S.M.E.
Bachelors
Engineering and Computer Science
Mechanical Engineering
32
Baker, Sarah. "A biocultural analysis of natural mummification : the importance of preservation on the examination of biological and cultural evidence." Honors in the Major Thesis, University of Central Florida, 2008. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1060.
Full textAbstract:
This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.Bachelors
Sciences
Anthropology
33
Mason, Steven M. "Life as a red blood cell in the artery of a cyborg." Honors in the Major Thesis, University of Central Florida, 1999. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/76.
Full textAbstract:
This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.Bachelors
Arts and Sciences
Humanities
34
Peterson, Lesley L. "E-ternally yours : the case for the development of a reliable repository for the preservation of personal digital objects." Honors in the Major Thesis, University of Central Florida, 2010. http://digital.library.ucf.edu/cdm/ref/collection/ETH/id/1476.
Full textAbstract:
This item is only available in print in the UCF Libraries. If this is your Honors Thesis, you can help us make it available online for use by researchers around the world by following the instructions on the distribution consent form at http://library.ucf.edu/Systems/DigitalInitiatives/DigitalCollections/InternetDistributionConsentAgreementForm.pdf You may also contact the project coordinator, Kerri Bottorff, at kerri.bottorff@ucf.edu for more information.Bachelors
Engineering and Computer Science
Information Systems Technology
35
Devine, Karen. "Unravelling the (DIS)CORD : Examining the social, legal and ethical implications of umbilical cord blood collection." Thesis, University of Kent, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.508568.
Full text
36
Duquette, Derek. "Queering Significance: What Preservationists Can Learn From How LGBTQ+Philadelphians Ascribe Significance to History Sites." Master's thesis, Temple University Libraries, 2018. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/491642.
Full textAbstract:
HistoryM.L.A.
This thesis explores the ways in which LGBTQ+ individuals in Philadelphia ascribe significance to various places based on oral history interviews and additional primary source material collected initially for the National Park Service Northeast Regional Office’s LGBTQ+ Heritage Initiative. By examining stories from LGBTQ+ individuals of places that matter most to them in Philadelphia, this thesis argues that historic preservationists can expand their definition of significance to include personal testimony and broaden their practices to better engage the communities whose histories they seek to preserve.
Temple University--Theses
37
Nalls, Michael A. "Admixture mapping and investigation of genetic associations of white blood cell count." Diss., Temple University Libraries, 2008. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/9412.
Full textAbstract:
AnthropologyPh.D.
This study analyzes variation in WBC among participants in the Health, Aging and Body Composition study (Health ABC). First, white blood cell (WBC) counts are compared among African Americans and European Americans in the study, confirming a significant difference. These population-based differences in WBC are also described in literature as differing significantly between different African populations, Caribbean populations, Central and South American groups, Asian populations and Europeans (Bain et al., 1984; Saxena and Wong, 1990; Bain, 1996; Menard et al., 2003; Hsieh et al., 2007). Ancestry informative markers are used to estimate the individual ancestry of the African Americans in the study. There is a significant association of low WBC with a higher proportion of African ancestry. An admixture mapping approach is used to identify a novel locus [independently identified in a separate admixture scan in the Jackson Heart Study (JHS)] that influences WBC levels. The peaks of association in both studies localize to a region ~0.9 Mb centered on the Duffy Antigen Receptor for Chemokines gene (DARC [MIM 110700]). Second, the functionality at the DARC locus is determined by investigating associations between DARC and neutrophils, lymnphocytes, monocytes, basophils and eosinophils. This investigation shows a consistent genetic effect at this locus associated with all of these differential cell types. Third, the association of DARC with all marrow derived cell lineages and red blood cell levels is evaluated. It is shown that, in addition to known associations between the DARC gene and malarial resistance (Miller et al., 1976; Livingstone, 1984) often associated with red blood cells, the DARC gene is also associated with WBC levels (Nalls et al., 2008). Selective pressure at this genetic locus may be related to its effect on WBC that may have contributed to the fixation of the FY- allele in some populations.
Temple University--Theses
38
Abd-Alla, Heba Ahmed. "African trypanosomes : a model for the improvement of molecular diagnosis of blood borne parasites." Thesis, University of Edinburgh, 2009. http://hdl.handle.net/1842/8735.
Full textAbstract:
Historically, diagnosis has relied on clinical signs of disease, microscopy and serological testing. However, these approaches have a number of drawbacks for example, differential diagnosis, low sensitivity (microscopy) and the inability to differentiate past from current infections (serology). In the past decade the use of molecular techniques, such as the polymerase chain reactions (PCR) have gained favour. Many research groups have used these techniques to study the molecular epidemiology of diseases in sub-Saharan Africa. Such methodologies rely on the detection of genetic materials and as such are reliant on the specificity of their components and the quality of the starting materials. It is the aim of this thesis is to demonstrate improvements that can be made to sample collection that will help to enhance the reliability of these tests and highlight the importance of the diagnostic parameters. The model that I will use to demonstrate these improvements are African trypanosomes, these are the causative agents of sleeping sickness in humans and nagana in animals, and are wide spread across much of sub-Saharan Africa. My work will be presented as three main sections: Firstly, a comparison of the suitability of various different approaches to cattle blood sample collection – including the genetic materials prepared directly in the field and the use of Whatman FTA®cards – in terms of the provision of appropriate materials for molecular screening will be presented. It was found that uneven distribution of genetic materials occurs across the surface of the FTA®cards due to the matrix chemistry. Therefore suggestions for improvements for the preparation of materials to be stored on these cards and their downstream application are made. Secondly, a comparison between the specificity of the pan-Trypanosoma ITS-PCR reaction and the species-specific reactions is made. The ITS-PCR has gained favour in recent years as it is reported to be capable of identifying a wide range of trypanosomes, as this is a single nested PCR reaction the reduction in time and cost has been very appealing to researchers in this field. My work suggests that this test is not reliable in terms of the accurate detection of trypanosomes species, and in fact on a direct comparison of 969 samples, 37 parasitic events where identified by this approach compared to 197 when species-specific tests were applied. Thirdly, based on my findings from the previous two chapters I present two case studies, the first of which looks to evaluate the impact on the prevalence of trypanosome species in cattle after drug treatment during the Ugandan, Stamp Out Sleeping sickness (www.sleepingsickness.com) campaign. The results of this case study highlight the importance of understanding the relationship that occurs between trypanosome species in mixed infections, my second case study therefore looks to quantifying the infection load of Trypanosoma brucei and T. congolense within the midgut of their insect vector (Glossina morsitans morsitans) using qPCR.
39
Osorio, Muriel Andres. "Improving the blood supply chain : simulation and optimisation models to support collection, production and location-allocation decisions." Thesis, University of Southampton, 2016. https://eprints.soton.ac.uk/404885/.
Full textAbstract:
This thesis introduces and studies di?erent problems in the blood supply chain. The problems are focused on aspects less frequently studied in the literature such as the exploitation of the di?erent collection and production alternatives, consideration of multiple products and uncertainty in demand and supply. These important features can be found in different decision levels, including daily collections, annual planning and at the strategic level when the blood supply chain is designed. For each problem presented, a suitable solution strategy is proposed. Different methods such as discrete event simulation, Monte Carlo simulation, optimisation, stochastic optimisation and multi-objective optimisation have been used to provide solutions to the problems studied. A simulation-optimisation model to support collection and production decisions in the blood supply chain is first presented. A model which integrated discrete event simulation and integer linear programming was designed to solve this problem. The model is tested using data from a blood centre in Colombia. Results show that key performance indicators such as total cost, number of donors, shortage and outdated units are improved by using the approach proposed. In addition, a stochastic multi-objective optimisation model to study the trade-off between cost and number of donors required is also included in this thesis. This model supports the decision of number of donors required by using whole blood and aphaeresis collection processes as well as considering the different blood groups and two main objectives: minimisation of cost and donors. The problem is solved using a combination of the augmented epsilon-constraint algorithm and the sample average approximation technique. A Pareto front considering stochastic demand is obtained by applying the proposed method. The final model included studies the optimal design of a blood supply chain as well as a discussion about the main motivations for centralised and decentralized systems. A stochastic mixed integer linear programming model is proposed and a solution method based on the sample average approximation technique is designed to address the problem. The complete approach is applied to a case study and several scenarios are generated to evaluate different travel time policies as well as the impact of using aphaeresis processes.
40
Damon, Deidre Erin. "Development of Functionalized Paper-Based Sample Collection and Direct Mass Spectrometry Analysis Platforms." The Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1550776934984565.
Full text
41
Carter, William. "RAMAN SPECTROSCOPIC STUDY OF SINGLE RED BLOOD CELLS INFECTED BY THE MALARIA PARASITE PLASMODIUM FALCIPARUM." Master's thesis, University of Central Florida, 2007. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/4269.
Full textAbstract:
Raman micro-spectroscopy provides a non-destructive probe with potential applications as a diagnostic tool for cellular disorders. This study presents micro-Raman spectra of live erythrocytes infected with a malaria parasite and investigates the potential of this probe to monitor molecular changes which occur during differentiation of the parasite inside the cell. At an excitation wavelength of 633 nm the spectral bands are dominated by hemoglobin vibrations yielding information the on structure and spin state of the heme moiety. It also demonstrates the novel use of silica capillaries as a viable method for studying the erythrocytes in an environment that is much closer to their native state, thus opening the possibility of maintaining the cell in vivo for long periods to study the dynamics of the parasite's growth.M.S.
Department of Physics
Sciences
Physics MS
42
Tang, GVGK. "The Surprise of a Knight: Excavating Material Legacies through Early Queer Film." Master's thesis, Temple University Libraries, 2019. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/567974.
Full textAbstract:
HistoryM.A.
Absent provenance or any background information, and with both implicit and explicit barriers to access within the archival space, how can we hypothesize—or critically fabulate—queer material legacies? The first—or earliest extant—American film to explicitly depict “queer” sex is The Surprise of a Knight (1929). By synthesizing perspectives on archives, material culture, queer identity, film, the Internet and pornography, this paper treats Surprise as an entry point into a discussion of public history and sexuality—revealing current issues with processing erotic materials and their impact on queer historiographies. This study outlines the problems presented by Surprise and explores contingencies for historical contextualization—methods public historians (archivists and interpreters alike) may adapt to fit similar materials within a broader history of film and queer identity. It explores current methods and future conundrums for best practices in the preservation of (born-digital) pornography, and concludes with impressions from potential audiences and present-day content producers as a means of envisioning new avenues of queer grassroots history-making.
Temple University--Theses
43
Diaz, Keith M. "Blood Pressure Variability: Relationship with Endothelial Health and Effects of an Exercise Training Intervention." Diss., Temple University Libraries, 2012. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/183386.
Full textAbstract:
KinesiologyPh.D.
Purpose: Evidence has accumulated to show that blood pressure variability (BPV) has a striking relationship with cardiovascular (CV) risk. Despite the mounting evidence implicating BPV as a CV risk factor, scant attention has been paid to: (1) the mechanisms by which high BPV confers greater CV risk; and (2) the efficacy of non-pharmacologic treatment modalities in the attenuation of BPV. In order to address these two unresolved questions, the purpose of this dissertation was twofold. The purpose of study #1 was to investigate the association between measures of short-term BPV (24-hour BPV) and long-term BPV (visit-to-visit BPV) with markers of endothelial health in a cohort of African Americans in order to determine if increased BPV may confer greater CV risk by eliciting injury to the endothelium. The purpose of study #2 was to investigate the effects of a 6-month aerobic exercise training (AEXT) intervention on visit-to-visit BPV and 24-hour BPV in the same cohort of African Americans in order to provide the first available data on the efficacy of a non-pharmacologic treatment modality in the lowering of BPV. Methods: We recruited 72 African Americans who were sedentary, non-diabetic, non-smoking, and free of CV and renal disease. Before and after a 6-month AEXT intervention, office blood pressure (BP) was measured at 3 separate visits and 24-hour ambulatory BP monitoring (ABPM) was conducted to measure visit-to-visit BPV and 24-hour BPV, respectively. Right brachial artery diameter was assessed at rest, during flow-mediated dilation (FMD), and after nitroglycerin-mediated dilation (NMD). Peak and area under the curve (AUC) were calculated as measures of FMD and NMD, and the FMD/NMD ratio was calculated as a measure of endothelial function normalized by smooth muscle function. Fasted blood samples were obtained and were analyzed for circulating EMPs expressed as CD31+CD42- and CD62E+ EMPs. Results: In study #1, participants with higher 24-hour diastolic BPV (DBPV) had significantly lower CD31+CD42- EMPs compared to participants with lower 24-hour DBPV. When categorized according to visit-to-visit DBPV, participants with higher visit-to-visit DBPV had a significantly lower FMD/NMD ratio, and significantly higher %NMDpeak and NMDAUCs compared to participants with lower visit-to-visit DBPV. When analyzed as continuous variables, 24-hour mean arterial pressure variability (MAPV) was inversely associated with CD31+CD42- EMPs visit-to-visit DBPV was inversely associated with the FMD/NMD ratio and positively associated with %NMDpeak and NMDAUC; and 24-hour DBPV was positively associated with NMDAUC. All associations were independent of age, gender, BMI, mean BP, and pulse pressure. In study #2 investigating the effects of AEXT in 33 participants who completed the study, 24-hour DBPV and 24-hour MAPV were significantly increased after AEXT. The increase in 24-hour DBPV was independent of changes in BMI, mean BP, and self-reported sleep time. Heart rate variability (HRV) derived from ABPM was associated with the changes in 24-hour DBPV and 24-hour MAPV. There were no significant changes in visit-to-visit BPV after AEXT. Conclusions: The results from study #1 provide evidence that BPV is associated with vascular health as endothelial function was decreased in participants with high visit-to-visit DBPV, while smooth muscle function was increased in participants with higher visit-to-visit and 24-hour DBPV. The findings from study #2 show that 6-months of AEXT do not elicit beneficial changes in BPV. The finding of an association between changes in 24-hour BPV with HRV could indicate, however, that changes in activity levels during ABPM, in part, contributed to the observed changes in 24-hour BPV.
Temple University--Theses
44
Berman, Michelle Lea. "Effects of Food Deprivation on Blood Lipid Concentration and Composition in Steller Sea Lions (Eumetopias Jubatus)." Master's thesis, University of Central Florida, 2005. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2958.
Full textAbstract:
Steller sea lions, the largest Otariid, fast during their breeding season; during this time they refrain from ingesting food for a period of 12-43 days. Fasting, while undertaking an extremely energetically demanding activity (breeding and pupping), requires specific physiological adaptations. This study examined the physiological response to fasting of two age classes, juveniles and sub-adults, during the breeding and non-breeding seasons to determine how these animals utilize lipids and the pattern of fatty acid mobilization from lipid stores during fasting. Four juveniles and 5 sub-adults were fasted for one and two weeks, respectively, and blood samples were collected approximately every 3 days for lipid analysis. The concentrations of plasma non-esterified fatty acids (NEFA) were analyzed spectrophotometrically. Serum fatty acid composition was analyzed using gas chromatography (GC) and their individual weight percent (wt %) were correlated with their peak retention time and calculated using the area under each peak. Sixty-nine fatty acids were quantified from each sample. However, only those with concentrations above 0.2 wt. % were included in the analysis. Sub-adult samples were grouped on a percent mass loss basis (0%, 7-8% and 15% mass loss) to facilitate comparison with the juveniles. These data represent the total lipid fatty acid composition of each blood sample. Relative lipid concentration was calculated by multiplying the total lipid fatty acid compositional analysis (wt %) by the NEFA concentrations measured in that respective blood sample. Plasma NEFA concentrations in juvenile Steller sea lions ranged from 1.2 [plus or minus] 0.51 mM to 3.7 [plus or minus] 0.69 during fasting and was within the range of fasting phocids. Concentrations of NEFAs in the sub-adult Steller sea lions ranged from 1.00 mM up to 9.70 mM and were generally higher than fasting phocids. The wt % of only one fatty acid (20:0) was significantly different between the breeding and non-breeding season in fasting juveniles. However, the wt % of seven fatty acids changed significantly during fasting in the juveniles and five of these were most significant in separating the beginning and end of the fasts using principal components analysis. In contrast, the wt % of 10 fatty acids were significantly different during the breeding and non-breeding season fasts of the sub-adults. Additionally, the wt % of 10 fatty acids changed significantly during fasting in the sub-adults and four of these (16:1n-7, 18:2n-6, 20:0, and 20:1n-9) were most significant in separating the beginning and end of the fasts using principal components analysis. These trends reveal the physiological differences between the juvenile and sub-adult Steller sea lions and suggest that the sub-adults may be better physiologically and metabolically adapted to fast than the juveniles in this study.M.S.
Department of Biology
Arts and Sciences
Biology
45
Diedrich, Beatrice. "Storage and transfusion of platelets in vitro and in vivo studies in healthy volunteers and in allogeneic hematopoetic progenitor cell transplant recipients /." Stockholm : Karolinska institutet, 2009. http://diss.kib.ki.se/2009/978-91-7409-280-6/.
Full text
46
Salvagnini, Claudio. "Thrombin inhibitors grafting on polyester membranes for the preparation of blood-compatible materials." Université catholique de Louvain, 2005. http://edoc.bib.ucl.ac.be:81/ETD-db/collection/available/BelnUcetd-11232005-103604/.
Full textAbstract:
The design of biomaterials, historically initiated and developed by physicians and engineers, in the last decades has slowly shifted toward a more biochemical based approach. For the replacement, repair and regeneration of tissues scientists are now focusing on materials that stimulate specific biological response at the molecular level. These biomaterials have already shown interesting applications in cell proliferation, differentiation, and extracellular matrix production and organization when the material modifications are designed to elicit specific interactions with cell integrins. In the present work we propose the application of this strategy for the development of blood-compatible materials. We first identified, in the coagulation cascade a key enzyme that constitute a valuable biological target for the development of anti-thrombogenic compounds. Piperazinyl-amide derivatives of N-alfa-(3-trifluoromethyl-benzenesulfonyl)-L-arginine were synthesized as graftable thrombin inhibitors. These inhibitors provided a spacer arm for surface grafting and a fluorine tag for XPS (X-ray photoelectron spectroscopy) detection. The possible disturbance of biological activity due to a variable spacer-arm fixed on the N-4 piperazinyl position was evaluated in vitro against human alfa-thrombin, in silico by molecular modelling and via X-ray diffraction study. Selected inhibitors, having inhibition potency in the mM range, were grafted on polyesters surface via wet chemistry and photochemical activation treatments. Wet chemistry surface grafting was performed by specific hydroxyl chain-ends activation and resulted in bioactive molecules fixation of 20-300pmol/cm2. The photochemical grafting was performed using a molecular clip providing an aromatic azide, for nitrene insertion into a polymer, and an activated ester for grafting of tag compounds. This grafting technique resulted in a dramatic increase in fixed bioactive signals (up to nmol/cm2). The material blood-compatibilization induced by the surface fixation of the inhibitors, was measured by a static blood clot weight measurement test. The wet chemistry grafting technique resulted in moderate blood-compatibilization while by the photochemical grafting method important decrease in surface blood clot formation was observed. In the latter case, the blood response to material contact was found to be strongly affected by the polyester surface photo-degradation induced by the activation treatment.
47
Petrick, Michael, Beth R. Mack, and Carrie Allen. "Evaluation of a Data Collection Form for Determining the Influence of Heparin Administration on Sepsis Severity in Patients with Candida Blood Stream Infections." The University of Arizona, 2006. http://hdl.handle.net/10150/624448.
Full textAbstract:
Class of 2006 AbstractObjectives: To develop and evaluate a data collection form that could be used to assess the influence of heparin administration on the septic severity index score in patients with Candida blood stream infections. Methods: A data collection form was developed to evaluate the influence of heparin administration on the septic severity index score in patients with Candida blood stream infections. This form was assessed using rating scales for ease of use, applicability of data collection items and availability of data in charts. Results: Data from 10 patient charts was used to assess the instrument. Patient demographics were similar. The strengths of the form included applicability of 9 out of 10 items in the instrument. Four out of 10 items were rated low for ease of use. In addition, 7 out of 10 pertinent data items were not documented in the charts. Conclusion: An extensive reworking of the data collection form as described in the discussion section was required. The form is ready to be implemented if the proposed study should be performed.
48
Kawata, Keisuke. "SUBCONCUSSIVE HEAD IMPACT EFFECT ON PLASMA EXPRESSION OF S100-BETA AND PINCH PROTEINS IN COLLEGIATE FOOTBALL PLAYERS." Diss., Temple University Libraries, 2016. http://cdm16002.contentdm.oclc.org/cdm/ref/collection/p245801coll10/id/398688.
Full textAbstract:
KinesiologyPh.D.
In this prospective longitudinal investigation of Division-I collegiate football players, the acute and longer-term effects of repetitive subconcussive impacts on plasma S100β and PINCH levels and concussion-related symptom score were examined. The first aim was to investigate the acute repetitive subconcussive impact effect by comparing the biomarker levels at pre and post full-gear practice, followed by examining the relationship of head impact magnitude and frequency of on acute increases in S100β and PINCH levels and symptom score. Hypotheses for the first aim were that there would be acute increases in plasma S100β and PINCH levels, but no change would be observed in symptom score. A significant relationship between subconcussive impact kinematics and acute changes in outcome measurements would be observed only in S100β. The second aim was to examine the longer-term effect of subconcussive effects on plasma S100β and PINCH levels as well as symptom score compared to the pre-season baseline. It was hypothesized that the players who sustained high frequency and magnitude of subconcussive impact would induce chronically high levels of plasma PINCH compared to the baseline. However, chronic effect would not be found in plasma S100β and symptom score. Independent variables were time (pre vs. post-practice), days (baseline, 1st Pads-OFF, 1st Pads-ON, 2nd Pads-ON, 3rd Pads-ON, 4th Pads-ON, and post-season), and group (higher vs. lower impact group). Dependent variables were the plasma expression of S100β and PINCH and symptom scores at each time point, pre-post differences in the plasma expression of S100β and PINCH and symptom scores, and head impact kinematics (frequency, sum of peak linear and rotational acceleration). This prospective observational study of 22 Division-I collegiate football players included pre-season baseline, pre-season practices [1 helmet-only and 4 full-gear], and post-season follow-up. Acute subconcussive effects were examined using the data from the first full-gear practice. Cumulative subconcussive effects were examined across the study duration (total 12 time points per player). Blood samples and self-reported symptom scores were obtained and blood biomarkers were assessed for pre-post practices and pre-post season. Plasma S100β expression level was assessed using a sandwich-based enzyme-linked immunosorbent assay. Plasma PINCH expression level was assessed using western blot analysis. An accelerometer-embedded mouth guard was employed to measure impact kinematics including number of impacts (hits), peak linear acceleration (PLA), and peak rotational acceleration (PRA). For examining cumulative effects, based on the previously established cut-off value of 173.5 g, players who were exposed average impact magnitudes below 173.5 g per practice were categorized into lower (n = 8) or greater than 173.5 g were categorized into higher (n = 14) impact groups. Data analysis consisted of descriptive and inferential statistics. Student’s t-tests were used to assess group differences in demographic and head impact kinematic data, acute effects using pre-post practice change in concussion-related symptom scores and biomarker levels, and longer-term effects using pre-post season change in concussion-related symptom scores and biomarker levels. Pearson r correlations were used to examine potential relationship between acute increase in outcome measures and head impact kinematics data. Two-way repeated measures ANOVAs were used to identify cumulative subconcussive effects over time in concussion-related symptoms scores and biomarker levels. If necessary, one-way ANOVA as a function of group was used to identify where cumulative effect began compared to the baseline, using Dunnett’s host-hoc correction. The alpha level was set at p < 0.05. A total of 721 head impacts were recorded from the 22 players during the 5 training camp practices. There were significant differences in head impact kinematics per practice between lower and higher impact groups [number of impacts per practice, 1.3 vs. 10.0 (p < .001); linear acceleration, 36.4 vs. 285.6 g (p < .001); rotational acceleration, 2,048.4 vs. 16,497.31 rad/s2 (p < .001), respectively]. There were no changes in self-reported concussion symptoms across the study duration. While there was no change in longer-term effect between pre-season baseline and post-season follow-up in plasma S100β level, robust and acute increase was observed in post-full gear practice (0.111 + 0.01 ng/ml) compared to pre-practice S100β level, (0.048 + 0.01 ng/ml; p < .0001). The acute increase in plasma S100β was significantly and positively correlated to the number of hits (r = 0.636, p = 0.001), sum of peak linear acceleration (r = 0.570, p = .006), and sum of peak rotational acceleration (r = 0.655, p = 0.001) sustained. For plasma PINCH level, there was a 4-fold increase at post-practice compared to that of pre-practice (p = .037), indicating the acute effect of subconcussive impacts. However, the acute increase in plasma PINCH level was independent from frequency and magnitude of impacts sustained, demonstrated by no statistically significant correlations with the number of hits (r = 0.222, p = .333), sum of peak linear acceleration (r = 0.289, p = .204), and sum of peak rotational acceleration (r = 0.297, p = .191). When players were categorized into the lower and higher impact groups and assessed across the 5 training-camp practices, consistently higher levels of plasma S100β and PINCH were found only in the higher impact group at post-practice compared to the baseline. However, plasma level of S100β and PINCH at pre-practice remained stable from the baseline, suggesting the absence of chronic effect from repetitive head impacts. When season-long effects on plasma S100β and PINCH levels were examined, 10 out of 16 players showed increase in plasma PINCH level at post-season compared to the baseline (p = .039) while no significant difference in plasma S100β level. Results from the current study suggest that subconcussive head impacts do not exert self-claimed concussion-related symptoms; however, blood biomarkers detected noticeable acute changes following repetitive subconcussive impacts. Plasma level of S100β protein can be a potential diagnostic measurement to track acute brain burden, and plasma level of PINCH protein may be reflective of the longer-term cumulative brain damage from repetitive head impacts.
Temple University--Theses
49
Holroyd, David. "Effect of blood flow on high intensity focused ultrasound therapy in an isolated, perfused liver model." Thesis, University of Oxford, 2015. https://ora.ox.ac.uk/objects/uuid:89f8f435-373d-46c2-92c8-5e9d21b4f01d.
Full textAbstract:
High intensity focused ultrasound (HIFU) is an emerging non-invasive thermal ablative modality that can be utilised for the treatment of solid organ tumours, including liver cancer. Acoustic cavitation is a phenomenon that can occur during HIFU and its presence can enhance heating rates. One major limitation of thermal ablative techniques in general, such as radiofrequency and microwave ablation, is the heat sink effect imparted by large vasculature. Thermal advection from blood flow in vessels ≥ 3 - 4 mm in diameter has been shown to significantly reduce heating rates and peak temperatures in the target tissue, potentially leading to treatment failure. With regards to HIFU therapy, a clearer understanding is required of the effects of blood flow on heating, cavitation and thermal tissue necrosis, which is the treatment endpoint in clinical thermal ablation. Therefore, the overall aim of this thesis project was to elucidate the effects of blood flow on HIFU-induced heating, cavitation and histological assessment of thermal ablation. A unique isolated, perfused porcine liver model was used in order to provide a relevant test bed, with physiological and anatomical characteristics similar to the in vivo human liver. The normothermic liver perfusion device used in all studies presented in this work can keep an organ alive in a functional state ex vivo for in excess of 72 hours. A further advantage of the liver perfusion device was that it allowed blood flow to be stopped completely and resumed rapidly, allowing studies to be conducted under zero flow conditions. A therapeutic HIFU system was used in order to deliver HIFU therapy to regions of hepatic parenchyma adjacent (≤ 3 mm) to large (≥ 5 mm) blood vessels or away from vasculature (≥ 1 cm) at either 1.06 MHz or at 3.18 MHz. Cavitation events during HIFU therapy were spatio-temporally monitored using a previously developed passive acoustic mapping (PAM) technique. The cavitation threshold at each frequency was determined through assessment of acoustic emissions acquired through PAM during HIFU exposure at a range of acoustic pressures. Real time thermal data during HIFU therapy were obtained using an implantable 400 μm thermocouple, aligned with the HIFU focus, in order to assess the effect of large vessel blood flow on peak tissue temperatures. Thermal data were obtained at 1.06 MHz, in the presence of acoustic cavitation and at 3.18 MHz, in the absence of cavitation, both in the presence and complete absence of blood flow. Finally, histological assessment of cell viability and cell death was performed in order to determine whether any heat sink effect could be overcome, with the achievement of complete tissue necrosis in treatment regions directly adjacent to large vasculature. This work demonstrated for the first time that in perfused, functional liver tissue, the presence of large vasculature and physiological blood flow does not significantly affect ablative HIFU therapy, both in terms of peak focal tissue temperatures attained and histological evidence of complete tissue necrosis. Therefore, HIFU may be superior to other ablative modalities in treating tumours in tissue regions adjacent to major vascular structures, but further work needs to be performed to correlate the experimental findings with clinical outcomes.
50
Gantt, MeLisa. "The Effect of Combat Exercises on Cardiovascular Response: An Exploratory Study." Doctoral diss., University of Central Florida, 2010. http://digital.library.ucf.edu/cdm/ref/collection/ETD/id/2232.
Full textAbstract:
PURPOSE: Hypertension (HTN) affects one in every three adults in the United States. Often associated with the older population, this silent killer has emerged in an unsuspecting group, young military soldiers. With the rapid succession of multiple deployments, long intervals between blood pressure (BP) assessments, and the absence of cardiovascular (CV) measures during the pre and post deployment health screenings; soldiers may be at higher risk for HTN than their civilian counterparts of the same age. The purpose of this study was to explore real-time continuous CV measures of soldiers before, during, and after exposure to a simulated combat stressor as well as to assess which personal characteristics played a significant role. METHODS: Applying the Allostasis/Allostatic Load theoretical framework, a repeated measure quasi-experimental design was used to compare the CV measures of two groups: one exposed to a physical combat stressor (experimental) and the other exposed to a psychological combat stressor (control). A convenience sample of 60 college Army Reserve Officer Training Corps cadets were fitted with Tiba Medical Ambulo 2400 ambulatory BP monitors for 48 continuous hours. Several CV indices were analyzed using one-way Analysis of Variance (ANOVA), paired t-test, and independent sample t-test. Four instruments (Participant Information Sheet, State-Trait Anxiety Inventory, Paintball Perceived Stress Questionnaire, and Army Physical Fitness Test) were used to assess which characteristics played the most significant role in the CV response. RESULTS: Demographic characteristics between the two 30-cadet groups were not statistically different, with the exception of deployment history (experimental = 4, control= 0). Hours after the stress exposure, subjects in the experimental group had a higher mean awake systolic blood pressure (SBP) when compared to the control group (113.76 mm Hg vs 102.98 mm Hg, p= 009). When assessing pre and post stressor morning BP surge and evening BP decline, the control group showed the biggest change. Significant bivariate correlations (p < .05) found that: Females consistently had higher pulse rates (PR) throughout each of the phases. Age positively correlated with elevated BP during the stressor, and increased morning BP surge post stressor. African Americans had higher sleep BPs pre stressor, and decreased SBP recovery post stressor; Hispanics had higher PRs during and after the stressor. Waist circumference positively correlated with elevated BP morning surge pre stressor but, body mass index (BMI) positively correlated with elevated BP morning surge post stressor as well as elevated BP during the stressor. Family history of HTN played no significant role; however, deployment history correlated with mean awake SBP post stressor. Trait anxiety scores negatively correlated with SBP recovery while state anxiety scores positively correlated with post stressor awake mean arterial pressure and evening SPB decline. Perceived stress during the stressor negatively correlated with post stressor SBP morning surge with females reporting higher anxiety and stress. Finally, Army Physical Fitness (APFT) total scores as well as APFT run scores negatively correlated with pre and post stressor sleep PRs. DISCUSSION/IMPLICATIONS: Age, female gender, African American descent, Hispanic ethnicity, waist circumference, BMI, previous deployment history, level of fitness and the psychological stress state during and after a stressor played significant roles in soldiers' CV response. SBP was the most dynamic and informative of the CV indices and a psychological stressor was comparable to a physical stressor when observing the residual effect of stress. If a combat exercise was enough of a stressor to produce residual CV effects that lasted well into following morning, the residual effect of a lengthy strenuous deployment may be alarming. This study not only provided a snapshot of the CV health of incoming young soldiers, but also provided evidence to support policy change regarding the implementation of regular CV health assessments before and after deployment.Ph.D.
School of Nursing
Other
Nursing PhD