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1
Sloan, Daniela. "Effects Of Ozone On Blood Components." Scholar Commons, 2010. https://scholarcommons.usf.edu/etd/1772.
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Previous studies on the medical use of ozone therapies show a very diverse array of results, from ozone reducing the amount of HIV virus in the blood, to no effect, to causing the death of several patients due to pulmonary embolism and infections. However, ozone therapies are widely used in Europe and considered medically safe. In the U.S., doctors in 28 states use ozone therapies.
The objectives of this study were to investigate the effects of medical grade ozone at varying concentrations used in ozone therapies. These were achieved by evaluating the C-reactive protein, erythrocyte sedimentation rate, total reduced and oxidized glutathione content of erythrocytes which were all markers used to determine ozone injury/inflammation.
Despite the fact that ozone is a very strong oxidant, previous research indicates that depending on the dose and the health status of the biological system, sometimes ozone can act as an antioxidant.
The medical exposure range for ozone is between 20-80 mg/ml with an average of 50 mg/ml. The concentrations used in this study were 20, 40, 80 and 160 mg/ml. Ozone was generated in the "Breath Lab" at USF from medical grade oxygen obtained through electrical corona arc discharge using an OL80C ozone generator. De-identified blood samples of 10 ml blood/sample containing EDTA as anticoagulant were obtained from the James A. Haley VA Hospital patients. Equal volumes of blood and ozone gas mixture were allowed to mix in ozone-resistant syringes prior to dividing each sample into three parts, one for each corresponding parameter to be studied. The C-reactive protein was analyzed through ELISA using the colorimetric method available from Helica Biosystems; erythrocyte sedimentation rate was measured in graduated sedimentation tubes; the total reduced glutathione (GSH) and oxidized glutathione (GSSG) content of erythrocytes was determined according to the colorimetric method developed by the Oxford Biomedical Research.
Overall, the concentrations of ozone used did not have a statistically significant effect on the parameters investigated. However, a small percentage of the blood samples showed an improvement in the parameters studied, especially at the highest ozone concentration.
2
Ljunggren, Lennart. "Biothermodynamic studies of blood components with special reference to biocompatibility." Lund : Dept. of Clinical Chemistry, University Hospital, 1994. http://catalog.hathitrust.org/api/volumes/oclc/39117042.html.
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Armstrong, F. H. "Tissue responses to perfluorochemical emulsion components in rats." Thesis, University of Nottingham, 1990. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.276220.
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Martins, Daniela Alexandra da Silva. "Clínica e cirurgia de animais de companhia." Master's thesis, Universidade de Évora, 2015. http://hdl.handle.net/10174/16868.
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O relatório aqui desenvolvido foca-se nos seis meses de estágio curricular realizado no Hospital Veterinário do Porto em clínica e cirurgia de animais de companhia. Este teve início a 1 de Agosto de 2014 e terminou a 31 de Janeiro de 2015, tendo-se realizado sob a orientação da Dra. Margarida Fragoso Costa e do Professor Dr Luís Lima Lobo, diretor clínico da instituição.
O mesmo encontra-se dividido em duas partes, tendo a primeira a finalidade de descrever as atividades acompanhadas no hospital através de uma descrição estatística dos casos acompanhados pela autora. A segunda parte engloba uma revisão bibliográfica sobre o tema “Medicina transfusional felina” e a exposição e referente discussão de dois casos clínicos sobre o tema, observados no período de estágio; Abstract: Small animal medicine and surgery
This report focuses on the six months of curricular internship that took place at Hospital Veterinário do Porto in general clinic duties and pet surgery. It began at August 1st 2014 and ended at January 31st 2015, under the guidance of Dr. Margarida Fragoso Costa as well as Dr. Luís Lima Lobo, the institution’s clinical director. The aforementioned is divided in two parts, having the first the objective of describing the activities accompanied by the author. The second part covers a bibliographical review on the theme "feline transfusional medicine" and the exposure and discussion of two clinical cases, related to the subject, which were observed during the internship.
5
Jones, G. R. D. "The interaction of the blood clotting factors with cellular components." Thesis, University of Oxford, 1987. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.376932.
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Solberg, Robert Glen. "Extracorporeal Circulation: Effect of Long-Term (24-Hour) Circulation on Blood Components." Thesis, Virginia Tech, 2010. http://hdl.handle.net/10919/32157.
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Extracorporeal circulation damages blood and causes harmful side effects such as stroke and/or systemic inflammatory response in patients. Reactions of blood components to extracorporeal circulation include complement and inflammatory reactions, coagulation and thrombogenesis, frank hemolysis, and platelet activation and adhesion to the extracorporeal circuit. Non-physiologic pressure and flow produced by blood pumps contribute to blood injury. Two pump types, roller and centrifugal, are used for maintaining flow, with various models available from different manufacturers. This study compared the effects of these two pumps in identical, isolated, artificial circuits to a non-pumped control for a period of 24 hours on heparinized porcine blood. Hematology parameters were used to evaluate blood damage. Mean corpuscular volume, mean corpuscular hemoglobin, white blood cell count, platelet count, and mean platelet volume were affected by time of circulation. Mean corpuscular hemoglobin, platelet count, and red cell distribution width were different between circulated and non-circulated blood, however no differences were found between the pumping systems in any parameter. Red blood cell count, total hemoglobin, and hematocrit were not affected by time or treatment. The changes observed in this study have implications for the use of extracorporeal circulation in the clinical setting and in future use of blood as a potential organ perfusion medium.Master of Science
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Ritchie, Helen. "Synthesis of components of the fibrinolytic system by human peripheral blood monocytes." Thesis, University of Aberdeen, 1995. http://digitool.abdn.ac.uk/R?func=search-advanced-go&find_code1=WSN&request1=AAIU078825.
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One feature of the atherosclerotic plaque is deposition of fibrin, suggesting an imbalance in the fibrinolytic system. Monocytes are the major infiltrating cell type during atherogenesis and can contribute to the balance of the fibrinolytic system by production of PAI-2, PAI-1, and u-PA. Production of these proteins by monocytes was investigated in this study. Monocytes synthesized PAI-2 in excess of PAI-1. PAI-2 expression was increased by thrombin. Upregulation of secreted and intracellular PAI-2 was maximal at a thrombin concentration of 1 U/ml. The increase in PAI-2 antigen was verified at the mRNA level. Thrombin upregulation of PAI-2 synthesis was comparable to LPS stimulation of PAI-2, although the dose effect of thrombin and LPS differed. Monocytes responded to native LDL, which is central to atherogenesis, by an upregulation in PAI-2 antigen and mRNA. The increase in PAI-2 was not dependent on modification of native LDL by monocytes, as assessed by incorporation of antioxidants. Stimulation of PAI-2 by monocytes following treatment with modified LDL was variable. Production of PAI-1, PAI-1 and u-PA from monocytes, macrophages and foam cells was compared. Macrophages and foam cells did not respond to thrombin or LPS by an upregulation in PAI-2 and all PAI-2 was intracellular. Intracellular PAI-2 in all cell types was not an active inhibitor of u-PA, unlike secreted PAI-2. Reduction or detergent treatment generated active PAI-2. The accumulation of intracellular PAI-2 was promoted by the presence of fetal calf serum in the culture medium. The upregulation in PAI-2 represents a potential imbalance in the fibrinolytic system, since no u-PA was detected. This may contribute to persistence of fibrin deposits in a local environment such as the atherosclerotic plaque.
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Abolarin, David. "Non-invasive Estimation of Blood Pressure using Harmonic Components of Oscillometric Pulses." Thesis, Université d'Ottawa / University of Ottawa, 2016. http://hdl.handle.net/10393/34114.
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This research presents a pulse-by-pulse analysis of Oscillometric blood pressure waveform at systolic, diastolic and mean arterial pressure points.
Using a mathematical optimization technique, pulses are characterized into component harmonic by minimizing the least square error. The results at the important pressure points are analyzed and compared for different subject using different waveform extraction techniques.
Blood pressure is estimated using the harmonic parameters. The approach studies changes in the parameters as oscillometric blood pressure recording is done. 8 harmonic parameters are obtained from the pulse characterization and are used to estimate Systolic arterial Blood Pressure, Mean arterial Blood Pressure, and Diastolic arterial Blood Pressure. The estimates are compared with our reference value to determine which has the best agreement. The proposed method is further compared with Maximum Amplitude Algorithm and Pulse Morphology Algorithm.
The effect of oscillometric waveform extraction methods on the proposed method is observed. The experiment established the fact that the extraction technique can alter the shape of oscillometric pulses. The methods were compared and it was observed that the used extraction methods did not make any significant difference on the accuracy, using this technique.
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Lindoff, Claes. "Haemostasis during pregnancy and perimenopausal age studies of fibrinolytic components and coagulation factors involved in vascular disease /." Lund : Dept. of Obstetrics and Gynaecology, Lund University, 1994. http://catalog.hathitrust.org/api/volumes/oclc/39750405.html.
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Анциферова, І. В., Владислав Вiкторович Любчак, Владислав Викторович Любчак, and Vladyslav Viktorovych Liubchak. "Виявлення критичних ланок в процесі підготовки компонентів крові до гемотрансфузії." Thesis, Сумський державний університет, 2016. http://essuir.sumdu.edu.ua/handle/123456789/47151.
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Згідно сучасних стандартів менеджменту та якості, якісна трансфузія можлива лише в разі забезпечення належного контролю на всіх етапах від донації до гемотрансфузії. Після еплементації угоди з ЄС, українські станції переливання крові почали переходити на європейські стандарти GMP. Однак, на сьогодні, в Україні не існує жодного нормативного документу, що регламентує належне розморожування та підігрів компонентів крові при ретрансфузії.
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Assunção, Marcelo Froes. "Avaliação do impacto institucional da implantação de um programa de Patient Blood Management (PBM), construção de um projeto educacional e revisão da literatura." Universidade de São Paulo, 2018. http://www.teses.usp.br/teses/disponiveis/17/17155/tde-13092018-152847/.
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Para muitos médicos de diversas especialidades, a transfusão é considerada a primeira linha na abordagem dos pacientes com anemia e perdas sanguíneas, apesar dos riscos atualmente reconhecidos. Patient Blood Management é uma forma de abordagem das transfusões baseada em um conhecimento multimodal e multidisciplinar, que tem como objetivo central a transfusão de hemocomponentes com base nas necessidades individuais dos pacientes, reduzindo a utilização de hemocomponentes alogênicos às situações em que não existem outras alternativas mais seguras e custo eficazes disponíveis. Neste estudo foram analisados indicadores de eficácia de implantação de um programa de Patient Blood Management, baseados nos pilares consagrados na literatura, particularmente no primeiro e terceiro pilares, que dependem de um envolvimento direto dos médicos para sua efetivação. A implantação de um novo protocolo ocorreu após divulgação interna, palestras e treinamentos para residentes e outros membros do corpo clínico interessados. O resultado do estudo mostrou que a adoção de um novo protocolo, baseado nos conceitos de Patient Blood Management não contribuiu para a mudança efetiva das práticas e em alguns indicadores houve piora em relação às práticas anteriores à sua implantação. Foi realizada também uma revisão bibliográfica sobre o tema e elaborado um programa educacional para estudantes, para os médicos e enfermeiros estudantes e profissionais não familiarizados com o tema.For many doctors of various specialties, transfusion is considered the first line in the approach to patients with anemia and blood loss, despite the currently recognized risks. Patient Blood Management is a way of approaching transfusions based on multimodal and multidisciplinary knowledge, whose main objective is the transfusion of blood components based on the needs of individual patients. This procedure reduces the use of allogeneic blood components in situations where there are safer alternatives and cost-effective solutions. In this study, we analyzed indicators of the effectiveness of the implementation of a Patient Blood Management program, based on the pillars established in the literature, particularly in the first and third pillars, which depend on a direct involvement of the physicians for their implementation. The use of a new protocol took place after an internal disclosure, a number of lectures and a training procedure for both residents and other members of the medical staff. The results of the study showed that the adoption of a new protocol, based on the concepts of Patient Blood Management, did not contribute to the effective change of practices. Some indicators pointed to a worsening of the practices after its adoption. A bibliographic review was also carried out on the topic and an educational program was developed for students, nurse students and professionals who are not familiar with the topic.
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Goode, Helen Frances. "The assessment of zinc in various blood components with relevance to body zinc status." Thesis, University of Leeds, 1989. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.236759.
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Picardo, M. "The interactions of porcine plasma lipoproteins with components of the arterial blood vessel wall." Thesis, Bucks New University, 1985. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.356213.
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Dai, Lingzhen. "Health Effects of PM2.5 and Its Components on Mortality, Blood Pressure, and DNA Methylation." Thesis, Harvard University, 2016. http://nrs.harvard.edu/urn-3:HUL.InstRepos:32644533.
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Epidemiological studies have examined the association between PM2.5 mass and mortality, but there remains uncertainty about the relative importance of species. PM2.5 contains various species, such as organic carbon, elemental carbon, and metals. Determining the differential toxicity of PM2.5 species and identifying species with greatest toxicity is of great importance to emission-control strategies and regulations.
In the dissertation thesis, effects of PM2.5 species on health outcomes on different levels were estimated. The first study examined the association between PM2.5 species and mortality on approximately 4.5 million deaths for all causes, cardiovascular diseases, myocardial infarction, stroke, and respiratory diseases in 75 U.S. cities for 2000-2006, using city-season specific Poisson regression and multivariate meta-regression controlled for infiltration. Since cardiovascular diseases are leading causes of death within U.S. population, the second study aimed to determine which PM2.5 species are associated with blood pressure, an indicator of cardiovascular health, in a longitudinal cohort. Linear mixed-effects models with the adaptive LASSO penalty were applied to longitudinal data from 718 elderly men in the Veterans Affairs Normative Aging Study (NAS), 1999-2010. Species considered included 8 metals (Fe, K, Al, Ni, V, Cu, Zn, and Na) and 3 non-metals (S, Si, and Se). At last, the relationship between long-term exposure to PM2.5 species and epigenome-wide DNA methylation at 484 613 CpG probes in the longitudinal NAS cohort that included 646 subjects were investigated to explore the potential biological mechanisms on the epigenetic level in study 3.
The studies have showed an increased risk of mortality and blood pressure associated with PM2.5, which varied with species, and differential DNA methylation linked to long-term exposure to particular components of PM2.5. In conclusion, mass alone might not be sufficient to evaluate the health effects of particles. Understanding the toxicity of particle components is crucial to public health.
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Picasso, Maria C., Jessica A. Lo-Tayraco, Juselly M. Ramos-Villanueva, Vinay Pasupuleti, and Adrian V. Hernandez. "Effect of vegetarian diets on the presentation of metabolic syndrome or its components: a systematic review and meta-analysis." Churchill Livingstone, 2018. http://hdl.handle.net/10757/624688.
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El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.Background & aims: Several studies have examined the effect of vegetarian diets (VD) on metabolic syndrome (MetS) or its components, but findings have been inconsistent. The aim of this study was to perform a systematic review and meta-analysis of randomized controlled trials (RCTs) and observational studies to assess the association between VD and MetS or its components (systolic blood pressure [SBP], diastolic blood pressure [DBP], fasting glucose triglycerides, waist circumference [WC], HDL-cholesterol (HDL-C)) in adults. Methods: The Cochrane Library, EMBASE, PubMed, Web of Science, and Scopus were searched. RCTs, cohort studies and cross-sectional studies evaluating the effects of VD on MetS or its components in adults, with omnivore diet as control group, were included. Random effects meta-analyses stratified by study design were employed to calculate pooled estimates. Results: A total of 71 studies (n = 103 008) met the inclusion criteria (6 RCTs, 2 cohorts, 63 cross-sectional). VD were not associated with MetS in comparison to omnivorous diet (OR 0.96, 95% CI 0.50–1.85, p = 0.9) according to meta-analysis of five cross-sectional studies. Likewise, meta-analysis of RCTs and cohort studies indicated that consumption of VD were not associated with MetS components. Meta-analysis of cross-sectional studies demonstrated that VD were significantly associated with lower levels of SBP (mean difference [MD] −4.18 mmHg, 95%CI −5.57 to −2.80, p < 0.00001), DBP (MD −3.03 mmHg, 95% CI −4.93 to −1.13, p = 0.002), fasting glucose (MD −0.26 mmol/L, 95% CI −0.35to −0.17, p < 0.00001), WC (MD −1.63 cm, 95% CI −3.13 to −0.13, p = 0.03), and HDL-C (MD −0.05 mmol/L, 95% CI −0.07 to −0.03, p < 0.0001) in comparison to omnivorous diet. Heterogeneity of effects among cross-sectional studies was high. About, one-half of the included studies had high risk of bias. Conclusions: VD in comparison with omnivorous diet is not associated with a lower risk of MetS based on results of meta-analysis of cross-sectional studies. The association between VD and lower levels of SBP, DBP, HDL-C, and fasting glucose is uncertain due to high heterogeneity across the cross-sectional studies. Larger and controlled studies are needed to evaluate the association between VD and MetS and its components.
Revisión por pares
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Orban, Tivadar. "Modeling the human prothrombinase complex components." Cleveland, Ohio : Cleveland State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=csu1210257089.
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Thesis (Ph.D.)--Cleveland State University, 2008.Abstract. Title from PDF t.p. (viewed on Oct. 8, 2008). Includes bibliographical references. Available online via the OhioLINK ETD Center. Also available in print.
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Portero, Cláudia Marcelo. "Prática transfusional segura no doente em serviço de urgência - o papel do enfermeiro." Master's thesis, Universidade de Évora, 2018. http://hdl.handle.net/10174/23449.
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A hemoterapia é essencial na assistência em saúde e é fundamental para cuidar de doentes a vivenciar processos complexos de doença crítica. O enfermeiro tem um papel imprescindível na cadeia transfusional, cuja prática se impõe como determinante na diminuição dos riscos a que os doentes estão sujeitos quando necessitam desta terapia.
O presente relatório pretende descrever as fases de um projeto no qual, junto de 52 enfermeiros, foram identificadas estratégias promotoras de uma prática transfusional segura no serviço de urgência. Posteriormente, foi elaborada e planeada a utilização de uma checklist sobre transfusão de componentes sanguíneos, e foi implementado um plano formativo, com abordagem de conteúdos relacionados com todo o processo transfusional.
De igual forma, pretende descrever e analisar detalhadamente a aquisição e o desenvolvimento de Competências de Mestre, Competências Comuns de Enfermeiro Especialista e Competências Específicas de Enfermeiro Especialista em Enfermagem Médico-Cirúrgica, na vertente da Pessoa em Situação Crítica; ABSTRACT:
Safe Transfusion Practice In The Emergency Room Patient – The Role Of The Nurse
Hemotherapy is essential in health care assistance and it is fundamental in the care for patients experiencing complex processes of critical illness. Nurses have an essential role in the transfusion chain, whose practice is a key determinant in reducing the risks patients are subjected to when they need this therapy.
This report aims to describe the phases of a project in which, with 52 nurses, were identified strategies to promote safe transfusion practice in the emergency department. Subsequently, a checklist on blood component transfusion was prepared and planned, and a training plan was implemented, with a content approach related to the entire transfusion process.
Likewise, it intends to describe and analyze in detail the acquisition and development of Master's Skills, Common Competences of Specialist Nurse and Specific Competences of Specialist Nurse in Medical-Surgical Nursing, in the perspective of the Critical Situation Person.
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Бордун, І. В., Ірина Петрівна Грибачова, Ирина Петровна Грибачева, and Iryna Petrivna Hrybachova. "Правові проблеми донорства крові та її компонентів в Україні." Thesis, Сумський державний університет, 2019. https://essuir.sumdu.edu.ua/handle/123456789/78150.
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Донорство крові це один із напрямів діяльності державної політики у сфері
охорони здоров‘я. Він включає в себе процес взяття, зберігання, транспортування та
реалізації донорської крові та її компонентів. Проте сьогодні не всі люди розуміють
важливість бути донором і скільки життів це може врятувати, адже переливання крові
лікарі роблять лише у виключних випадках, коли іншого шляху допомогти пацієнту
немає. Крім цього донорська кров також використовують для винайдення ліків та у
наукових цілях. Це питання досліджували М. Щирба, Г. Чеботарьова, Т. Данилюк,
К. Ільющенкова, Г. Білецька та інші.
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Stenzel, D. "Functional diversity of extracellular matrix components during vessel migration, endothelial sprouting and blood vessel stabilization." Thesis, University College London (University of London), 2009. http://discovery.ucl.ac.uk/18782/.
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The formation of mature blood vessels requires recruitment of mural cells (MC) and generation of an extracellular matrix in order to stabilize and support the nascent vessel. The precise sequence of basement membrane protein expression and function during initiation, elongation and stabilization of the angiogenic sprout remains unclear. The first project describes a function of astrocytic Fibronectin (FN) in guided vessel migration. Integrin-binding is dispensable for fibrilliar FN assembly but mediates endothelial cell adhesion in vivo. VEGF-A dependent migration of retinal vessels requires PI3K activity and astrocytic FN possibly functions to retain VEGF protein on the astrocytic matrix. In a second project we discovered endothelial tip cells-specific expression of laminin alpha4 (Lama4) and provide first evidence for an influence of laminin α4 on endothelial Dll4/Notch signalling in sprouting angiogenesis. Loss of Lama4 leads to increased filopodia formation, tip cell numbers and consequently increased vessel density, resembling the phenotype of disturbed Dll4/Notch signalling. Loss of Lama4 leads to reduced Dll4 and Notch target gene expression, whereas gain-of-function results in increased Dll4 expression in vitro. Preliminary results suggest that laminin α4 induced Dll4 expression involves both VEGFR2 and integrin signalling. The third project addresses the cell-autonomous requirement of heparan sulfate (HS) production by MCs during mouse embryonic vascular development. Conditional deletion of HS synthesis in MC caused severe vascular defects and embryonic lethality. Unexpectedly, distinct regions of vascular growth showed selective requirement for MC HS production: whereas MC recruitment in peripheral skin vascularization is severely disrupted, MCs during brain angiogenesis appear unaffected. Similarly, PDGF-B and TGF-β signalling are impaired in the peripheral vessels, but not in the brain. These data suggest that cell-autonomous HS is essential for MC recruitment in the skin vasculature, where induction and differentiation of local progenitor cells from the mesenchymal cell lineage is mediated by TGF-β signalling.
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Upile, Tahwinder. "The adverse effects of stored blood transfusion components in head and neck squamous cell cancer." Thesis, University of Manchester, 2009. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.518437.
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Anderson, Julia A. M. "A hypersulphated oligosaccharide inhibits intrinsic tenase and prothrombinase : key components of the blood coagulation cascade." Thesis, University of Edinburgh, 1998. http://hdl.handle.net/1842/21290.
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The action of thrombin is central to the processes of thrombosis and haemostasis. Thrombin is generated following the activation of prothrombin by 'prothrombinase' (II-ase), the phospholipid membrane-bound factor Xa (fXa)-factor Va (fVa) complex, which in turn is dependent upon the generation of fXa by 'intrinsic tenase' (X-ase), the phospholipid membrane-bound factor IXa (fIXa)-factor VIIIa (fVIIIa) complex. Thrombin not only converts fibrinogen to fibrin, but also amplifies its own formation by activating the cofactors in II-ase and X-ase, factor V and factor VIII, respectively. The critical role played by II-ase and X-ase towards thrombin generation makes these membrane-bound enzyme complexes attractive targets for inhibition and offers an effective approach to blocking coagulation. Heparin acts as an anticoagulant by activating antithrombin (AT), which then inactivates thrombin, fXa and other activated clotting factors. However, the heparin-AT complex has limited activity against membrane-bound fIXa and fXa. Recently, in buffer systems, heparin has been shown to have an AT-independent effect on coagulation by directly inhibiting X-ase, an effect that is minimal in plasma where the AT-dependent heparin effect predominates. To capitalise on this AT-independent effect, heparin was chemically modified by periodate oxidation and borohydrate reduction to lower its affinity for AT (from Kd value of 25nM to 43μM); we used LMWH rather than heparin to take advantage of the superior pharmacokinetic profile of LMWH. Using this low affinity LMWH (LA-LMWH), N-desulphated LMWH was prepared using a solvolytic desulphation method. Whereas LA-LMWH inhibited X-ase to a similar extent as LMWH with high AT-affinity (IC50 of 16μg/ml and 13μg/ml respectively), N-desulphated LMWH had minimal inhibitory activity (IC50 of 166μg/ml).
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Liu, Hanhan [Verfasser], and Nikolaus [Akademischer Betreuer] Plesnila. "The role of blood components in microcirculatory dysfunction after subarachnoid hemorrhage / Hanhan Liu ; Betreuer: Nikolaus Plesnila." München : Universitätsbibliothek der Ludwig-Maximilians-Universität, 2018. http://d-nb.info/1176409662/34.
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Avent, N. D. "Isolation and characterization of erythrocyte membrane components that express the rhesus and related blood group antigens." Thesis, University of Bristol, 1988. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.233769.
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Zhang, Xuguang. "Blood cell-derived microparticles as a potential index of inflammatory processes, and their modulation by components of the diet." Thesis, University of Aberdeen, 2013. http://digitool.abdn.ac.uk:80/webclient/DeliveryManager?pid=203794.
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Musto, Anthony. "The Effects of an Incremental Pedometer Program on Metabolic Syndrome Components in Sedentary Overweight Women." Scholarly Repository, 2008. http://scholarlyrepository.miami.edu/oa_dissertations/93.
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Objective: Pedometer programs are effective in increasing physical activity in sedentary individuals, a population that is at an increased for developing metabolic syndrome and each of its individual components. The aim of this study was to observe the effects of incremental increases in pedometer assessed physical activity on MetS components in sedentary overweight women. Design: This study was a longitudinal, quasi-experimental design. Participants were recruited from a 12-week work-site pedometer program and grouped after the intervention based on their self-paced increases in pedometer assessed physical activity with (a) active participants that increased their daily activity by mean volume >3000 steps/day or (b) participants who remained sedentary. Subjects: A multi-ethnic heterogeneous sample of seventy-seven sedentary middle-aged women (BMI: 30.15 plus or minus 5.24 kg/m2, age: 46.00 plus or minus 9.93 years). Measurements: Self-reported physical activity, pedometer assessed physical activity (steps/day), body mass index, resting heart rate, waist circumference, blood pressure, triglycerides, HDL-C and fasting glucose at baseline and after the 12-week program. Results: The active group showed significant within-group improvements (P less than or equal to .01) in waist circumference and fasting glucose. Significant between group changes were observed in resting heart rate (P less than or equal to .01), weight (P less than or equal to .01), BMI (P less than or equal to .01), and systolic blood pressure (P less than or equal to .001); however, the changes observed in systolic blood pressure were not independent of weight loss. Conclusions: Our study showed that incremental increases in steps/day over 12 weeks is effective in inducing mild, but favorable changes in some MetS components. This data suggests that this approach to increasing step/day is a viable starting point for sedentary individuals that may find it difficult to initially accumulate 10,000 steps/day.
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Clements-Jewery, Hugh. "Examination of the role of catecholamines and blood components in mediating arrhythmias during evolution of myocardial infarction." Thesis, King's College London (University of London), 2003. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.408440.
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Vicente, Claudia Spegiorin 1962. "Produção mais limpa aplicada nos processos de produção e transfusão de hemocomponentes." [s.n.], 2014. http://repositorio.unicamp.br/jspui/handle/REPOSIP/258314.
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Orientador: Egle Novaes TeixeiraTese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia Civil, Arquitetura e Urbanismo
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Resumo: Os hemocomponentes, concentrados de hemácias, concentrados de plaquetas e plasma, são os produtos obtidos a partir do sangue total doado pelo doador de sangue. São os principais produtos de um serviço de hemoterapia, e devem ter características que garantam a segurança da terapia transfusional. No acompanhamento da cadeia geradora de resíduo do Hemocentro de Campinas, o descarte de unidades de sangue total e de hemocomponentes chama a atenção. A Metodologia de Produção mais Limpa (P+L) é uma ferramenta interessante para tratar as causas destes eventos, pois, através dela, é possível analisar as causas de geração deste resíduo, promover a minimização e a busca por soluções que tragam benefícios sociais, econômicos e ambientais. Neste estudo foi analisado o fluxo das bolsas plásticas para coleta de sangue de doadores desde a aquisição até a finalização da transfusão, tendo como referência a metodologia da P+L para identificar as causas e as possibilidades de minimização do descarte de unidades sangue total e de hemocomponentes em cada processo. Foram identificadas seis oportunidades de P+L, uma no processo de Gestão de Materiais, três no processo de Coleta de Sangue Total, duas no processo de Processamento, e uma no processo do Laboratório de Compatibilidade. Os respectivos planos de monitoramento definidos incluíram ações de gerenciamento dos processos de trabalho, mudança de técnica e mudança de material. As principais barreiras identificadas estão relacionadas aos recursos econômicos, ao dimensionamento de recursos humanos, e ao planejamento de ações de captação de doadores e coleta de sangue total sem considerar a capacidade operacional da unidade de processamento. Na análise do lay out da área física e do fluxo de atividades da sala de coleta identificou-se pontos críticos que induzem ao erro e podem gerar o descarte. Conclui-se que a aplicação da Metodologia P+L possibilita a identificação das causas de descarte e das quantidades descartadas, as ações propostas podem promover a minimização da geração deste resíduo e no acompanhamento das ações propostas e dos resultados, definidos nos planos de monitoramento para cada oportunidade, cria-se um ciclo ininterrupto da aplicação contínua de uma estratégia preventiva de geração de resíduo, tal qual preconizada na definição de P+L feita pela UNIDO
Abstract: Blood components, packed red cells, platelet concentrates and plasma, are the products obtained from whole blood donated by blood donors. These are the main products of a transfusion service, and must have features that ensure the safety of transfusion therapy. In monitoring the chain of waste generated by the Blood Center of Campinas, the disposal of units of whole blood and blood components draws attention. The Methodology of Cleaner Production (CP) is an interesting tool for addressing the causes of these events, enabling the analysis of the causes of this waste generation, promoting the minimization of this waste and for seeking solutions that bring social, economic and environmental benefits. In this study the flow of plastic bags for blood donors was analyzed; from acquisition to completion of transfusion, using the P + L methodology as the reference to identify the causes of the disposal of units of whole blood and blood products and possibilities for minimizing waste discard in each process. Six opportunities of CP were identified, one in the process of Material Management, three in the process of Whole Blood Collection, two in the process of blood processing, and one in the Compatibility Laboratory process. Monitoring plans included management actions, defined work processes, technical changes and material changes. The main barriers identified were related to economic resources, design of human resources, donor recruitment planning and collection of whole blood without considering the operational capacity of the processing unit. During the analysis of the physical layout of the area and the flow of activities in the collection room, critical points inducing error and generating waste were identified. We concluded that the application of the CP methodology enabled the identification of the causes of waste and of the quantities discarded, moreover the actions proposed could promote minimization of waste generation and that the monitoring of proposed actions and outcomes, defined in the monitoring plans at every opportunity, created a continuous cycle of application of preventive strategies for waste generation, as recommended by UNIDO
Doutorado
Saneamento e Ambiente
Doutora em Engenharia Civil
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Rodriguez, Luis Alberto. "Effect of dietary protein degradability and fat on rumen, blood and milk components of Jersey and Holstein cows." Thesis, This resource online, 1994. http://scholar.lib.vt.edu/theses/available/etd-01312009-063012/.
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Mallinson, Gary. "Characterisation of the erythrocyte membrane components which carry the antigens of the LW, Duffy and Cromer blood group systems." Thesis, University of the West of England, Bristol, 1995. http://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.294721.
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Gonzales, Raoul F. "The contribution of blood components to myocardial ischemia reperfusion injury and the benefits of vitamin E in the diabetic heart." Diss., The University of Arizona, 2003. http://hdl.handle.net/10150/289974.
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Type 2 diabetes is a major risk factor in cardiovascular disease especially ischemic heart disease. The American Diabetes Association advocates that all diabetics are at risk for coronary myocardial infarctions (MI). Restoration of blood flow to the area of the myocardium that has undergone ischemia is the most important therapeutic goal in treating MI. However, restoration of blood to the myocardium contributes to further damage to the ischemic area. This phenomenon is known as ischemia-reperfusion (I-R) injury. Previous studies have found that experimentally induced diabetic animals are more susceptible to I-R injury compared to non-diabetic animals. One reason why diabetic hearts may be more susceptible to I-R injury may be due to the increased oxidative stress in diabetics. Diabetics also suffer from increased blood coagulation possibly due to increased thrombin activity. Thrombin, independent of its role in coagulation, may be contributing to additional myocardial damage following ischemia. This dissertation describes three experiments: First, determine if a non-thrombogenic dose of thrombin impairs cardiac recovery and compliance in isolated non-diabetic rat hearts using a red cell perfusate following 30 minutes of global ischemia. Second, determine if type 2 diabetic rats exhibit increased platelet microparticle formation (PMP), increased thrombin activity and increased coagulability. Third, determine if MI's were larger in diabetic rat hearts compared to non-diabetics and if vitamin E reduced MI size and improve cardiac function following coronary artery occlusion. We found that a non-thrombogenic thrombin dose significantly reduced recovery from global I-R and reduced myocardial compliance. Thrombin may act as a contributing factor in cardiovascular pathology independent of its role in coagulation. We found that thrombin production is increased 30 fold in type 2 diabetic rats compared to non-diabetic rats and PMP production is significantly increased in diabetics possibly resulting in a hypercoagulable state. Finally, we show that MI's are significantly larger in type 2 diabetic hearts compared to non-diabetics. Treatment with Vitamin E significantly reduced MI size and improved left ventricular function in treated diabetic rats compared to non-treated rats. These results help to explain the increased cardiovascular injury seen in the diabetic heart. Vitamin E may be a useful adjuvant to help diabetics reduce oxidative stress, minimizing cardiac damage done by oxidation compounds and activated blood coagulation components in the diabetic heart.
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Flam, Brenda R. "Functional topology and regulation of endothelial nitric oxide synthase and associated caveolar components." [Tampa, Fla] : University of South Florida, 2006. http://purl.fcla.edu/usf/dc/et/SFE0001736.
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Otaviano, Divino José. "Avaliação sorológica para doenças infecciosas transmissíveis por transfusão em receptores de sangue do Hospital Geral de Palmas - TO." Universidade de São Paulo, 2015. http://www.teses.usp.br/teses/disponiveis/17/17155/tde-07012016-101949/.
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A legislação brasileira obriga a realização de testes sorológicos em todas as amostras de sangue de doadores, com o objetivo de prevenir a transmissão transfusional de microorganismos causadores de doenças como hepatite B, hepatite C, síndrome da imunodeficiência adquirida (SIDA), doença de Chagas, HTLV I/II e sífilis. Este procedimento é importante, porém não é suficiente para garantir a segurança do procedimento transfusional, sendo assim durante todo o ciclo do sangue, atitudes são adotadas para minimizar sinistros no objetivo final que a transfusão. Para receptores, a mesma legislação só obriga a realização de testes imuno-hematológicos pré-transfusionais, o que não revela o perfil sorológico deles, que por vezes pode ser positivo para algum dos marcadores antes da transfusão. O objetivo do presente trabalho foi avaliar o perfil sorológico pré-transfusional para doenças infecciosas transmissíveis por transfusão sanguínea em receptores de sangue de um hospital brasileiro de referência, o Hospital Geral de Palmas (HGP), no Estado do Tocantins. Foram realizados testes sorológicos pré-transfusionais para SIDA, hepatite B, hepatite C e sífilis em 514 receptores de transfusão do HGP, no período de fevereiro de 2014 a agosto de 2014. Vinte e nove receptores (5,65%) apresentaram-se reagentes para pelo menos um dos marcadores sorológicos testados. Os receptores apresentaram sorologia positiva para hepatite B (1,57%), hepatite C (0,58%), SIDA (1,17%) e sífilis (2,33%). Dentre os receptores de sangue que apresentaram sorologia positiva para alguma das doenças infecciosas testadas, 37,93% foram atendidos no centro cirúrgico e 27,59% na unidade de tratamento intensivo do HGP. Dentre os receptores com sorologia positiva, 62% não haviam sido transfundidos anteriormente. Este estudo determinou a prevalência de doenças infecciosas em receptores de sangue de um hospital brasileiro de referência, bem como discutiu a viabilidade e importância da implementação de testes sorológicos pré-transfusionais em receptores de sangue no Brasil. A avaliação sorológica pré-transfusional tem grande relevância pois possibilita o diagnóstico precoce de infecções nos receptores de sangue, minimizando assim o risco de comorbidade. Por outro lado, confere uma maior segurança judicial para os Serviços de Hemoterapia e para o Estado, quando da necessidade de esclarecimentos sobre possíveis transmissões de doenças infecciosas por transfusão sanguínea.Brazilian law requires serological tests in all donors of blood samples, in order to prevent transfusion transmission of disease-causing organisms such as hepatitis B, hepatitis C, acquired immunodeficiency syndrome ( AIDS), Chagas disease, HTLV I/II and syphilis. This is important, but not sufficient to ensure the safety of the transfusion procedure, so all the blood cycle, actions are taken to minimize losses in the ultimate goal that the transfusion. For receivers, the same legislation only requires the completion of pre-transfusion immuno-hematological tests, which does not reveal the serological profile of them, which can sometimes be positive for any of the markers prior to transfusion. The objective of this study was to evaluate the pre-transfusion serological profile for transfusion-transmitted infectious diseases in blood recipients from reference Brazilian hospital, Palmas General Hospital (HGP), State of Tocantins, Brazil. Pre-transfusion serological tests for HIV, HBV, HCV and syphilis were performed in 514 transfusion recipients of HGP, from February 2014 to August 2014. Twenty and nine receivers (5.65%) presented reactivity for at least one of the evaluated serological markers. The recipients were positive for hepatitis B (1.57%), hepatitis C (0.58%), AIDS (1.17%), and syphilis (2.33%). Among the blood recipients with positive serology for any of the tested infectious diseases, 37.93% were treated at the surgical center and 27.59% in the intensive care unit of the HGP, and 62% of recipients had not been previously transfused. Thus, this study determined the prevalence of infectious diseases in blood recipients from a Brazilian reference hospital, and discussed the feasibility and importance of implementing pre-transfusion serological tests for blood recipients in Brazil. The pre-transfusion serological evaluation is important because it provides both an early diagnosis of infections in blood recipients, thus minimizing the risk of comorbidity, and a greater legal certainty for Hematology Services and for the State, when clarification about possible transmissions of infectious diseases by blood transfusion are needed.
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Consolo, Filippo, Jawaad Sheriff, Silvia Gorla, Nicolò Magri, Danny Bluestein, Federico Pappalardo, Marvin J. Slepian, Gianfranco B. Fiore, and Alberto Redaelli. "High Frequency Components of Hemodynamic Shear Stress Profiles are a Major Determinant of Shear-Mediated Platelet Activation in Therapeutic Blood Recirculating Devices." NATURE PUBLISHING GROUP, 2017. http://hdl.handle.net/10150/625171.
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We systematically analyzed the relative contributions of frequency component elements of hemodynamic shear stress waveforms encountered in cardiovascular blood recirculating devices as to overall platelet activation over time. We demonstrated that high frequency oscillations are the major determinants for priming, triggering and yielding activated "prothrombotic behavior" for stimulated platelets, even if the imparted shear stress has low magnitude and brief exposure time. Conversely, the low frequency components of the stress signal, with limited oscillations over time, did not induce significant activation, despite being of high magnitude and/or exposure time. In vitro data were compared with numerical predictions computed according to a recently proposed numerical model of shear-mediated platelet activation. The numerical model effectively resolved the correlation between platelet activation and the various frequency components examined. However, numerical predictions exhibited a different activation trend compared to experimental results for different time points of a stress activation sequence. With this study we provide a more fundamental understanding for the mechanobiological responsiveness of circulating platelets to the hemodynamic environment of cardiovascular devices, and the importance of these environments in mediating life-threatening thromboembolic complications associated with shear-mediated platelet activation. Experimental data will guide further optimization of the thromboresistance of cardiovascular implantable therapeutic devices.
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Binoti, Dione Henrique Breda. "Perfil protéico de vacas de grupos genéticos holandês X gir de segunda lactação." Universidade Federal do Espírito Santo, 2011. http://repositorio.ufes.br/handle/10/5843.
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Previous issue date: 2011-08-30Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
The dairy farming is practiced throughout the national territory and approximately 70% of milk production in Brazil comes from crossbred animals derived from European dairy cattle breeds with zebu breeds of excellent adaptation to tropical conditions. Due the metabolic profile studies have focused on specialized pure breeds in this research was aimed to assess the metabolic profile of protein in two genetic groups of Holstein x Gir cows, from the second lactation order, in two periods of lactation, during the dry season. This work was carried out at the Fazenda Santa Luzia, belonging to the Grupo Cabo Verde, Passos - MG. Data were collected between May and August 2009, from ½ HG cows (37) and ¾ HG cows (35). Two periods of lactation were studied: from 28 to 60 and from 110 to 130 days. The feeding of the herd was conducted in accordance with milk production. From each animal, fasting, 10 mL of blood without anticoagulant, and 4.5 mL, with EDTA anticoagulant were collected. Milk yield and body condition score were evaluated and serum levels of urea, albumin, total protein and hemoglobin determined using a manual spectrophotometer, based on specific procedures for each component. Results showed that milk production was higher in animals ¾ HG in both periods of lactation and body condition score higher in the animals of the second period of lactation, but similar when comparing genetic groups. Although had occurred differences among lactation periods to the genetic groups for urea, albumin and total protein, results for these variables were similar as well as for hemoglobin into genetic groups. However genetic groups presented different results from those obtained for purebreds, demonstrating the need particularly to reconsider the nutritional management of these groups, when related to the purebreds. So, it s suggested that others research could be done in these conditions in order to elucidate this uncertain and to enable to construct tables with specific reference values for these genetic groups
A atividade leiteira é praticada em todo o território nacional e aproximadamente 70% da produção de leite do Brasil provem de animais derivados de cruzamentos de raças européias especializadas para produção de leite com raças zebuínas de excelente adaptação às condições tropicais. Estudos de Perfil Metabólico têm se concentrado em raças especializadas puras, assim objetivou-se aferir o perfil metabólico protéico de dois grupos genéticos de vacas Holandês x Gir, de segunda ordem de lactação, em dois períodos da lactação, na estação seca do ano. Também se procurou relacionar os resultados com os de estudos com raças puras. Foi conduzido na Fazenda Santa Luzia, pertencente ao Grupo Cabo Verde, Passos - MG. Os dados foram coletados entre maio e agosto de 2009, em vacas ½ HG (37) e vacas ¾ HG (35). Foram estudados dois períodos da lactação: de 28 a 60 e de 110 a 130 dias. O manejo alimentar do rebanho foi conduzido de acordo com a produção de leite. Foram coletados, de cada animal, em jejum, 10 mL de sangue, sem anticoagulante, e 4,5 mL, com anticoagulante EDTA. Foram avaliados a produção de leite e o escore de condição corporal e determinados os teores séricos de uréia, albumina, proteínas totais e hemoglobina em espectrofotômetro manual, baseado em procedimentos específicos para cada componente. Os resultados mostraram que a produção de leite foi mais elevada em animais ¾ HG, em ambos os períodos da lactação e o escore de condição corporal mais elevado nos animais do segundo período da lactação, mas semelhantes quando se comparam grupos genéticos. Embora entre períodos da lactação tenham ocorrido diferenças dentro de cada grupo genético para uréia, albumina e proteínas totais, entre os dois grupos genéticos os resultados para essas variáveis, assim como para hemoglobina, foram semelhantes. Contudo, os dois grupos genéticos apresentaram resultados distintos dos obtidos com raças puras, demonstrando a necessidade de se reconsiderar o manejo, principalmente nutricional, desses grupos genéticos em relação às raças puras. Assim, sugere-se que outras pesquisas possam ser realizadas, visando a elucidar essa incerteza e possibilitar a construção de tabelas de valores de referência específicas para esses grupos genéticos
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Müller, Laura [Verfasser]. "Using the interactions of designed siRNA and DNA drug carrier systems with human blood plasma and its components for controlled drug delivery / Laura Müller." Mainz : Universitätsbibliothek Mainz, 2017. http://d-nb.info/1141265273/34.
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Österlind, Per Olov. "Medical and social conditions in the elderly gender and age differences : the Umeå longitudinal study." Doctoral thesis, Umeå universitet, Geriatrik, 1993. http://urn.kb.se/resolve?urn=urn:nbn:se:umu:diva-102560.
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In 1981, no representative study of the medical and social conditions among elderly persons in northern Scandinavia was performed. Nor was there such a study in a smaller Swedish town than Gothenburg. This study was initiated to fill that knowledge gap. The aim of the study was to evaluate gender and age differences in medical and social conditions among elderly people, including the reference intervals of clinical chemistry parameters and characteristic features of the 24-hour electrocardiogram (ECG) in healthy elderly. By way of longitudinal design with birth cohorts stratified by gender, it was assumed that the effects could be demonstrated. Also, the death risk of various social and medical variables was to be assessed. During the study period 1981 to 1990, the subjects were between 70 and 88 years of age. The proportion of persons living in private housing decreased from almost all at the age of 70 to slightly more than half at 88 years of age. The number of socially active persons decreased considerably during the period. The need of help increased from almost none to 60 % of the persons. The proportions of persons with normal sight and hearing decreased from two thirds to around one tenth The most frequent symptoms were general tiredness, pains, dyspnoea, constipation and dryness of the mouth. Cardiovascular diseases were the most frequent. Hypertensive disease became less, and congestive heart failure more frequent with age. The frequency of dementia increased steeply among the oldest persons; at 88 years of age, 40 % were demented. Drug consumption increased; the oldest persons in both age cohorts used 5 different drugs or more per person regularly. The consumption increase was mainly due to the increasing morbidity accompanying age. The most common drugs taken were cardiovascular preparations, psychoactive agents, drugs to alleviate gastrointestinal symptoms, and analgesics. Drug intake and symptom prevalence were generally higher in women, despite the fact that there was no gender difference in the number of diseases. The reference intervals of many blood components in healthy elderly were shown to be broader than those of younger persons. The intervals of P(lasma)-folate and P- potassium were on a lower and those of the erythrocyte sedimentation rate, P-creati- nine and, in women, S(erum)-cholesterol, were on a higher level than among younger persons. Several features of the 24-hour ECG, e.g. the number of episodes of supraventricular tachycardia as well as supraventricular and ventricular premature beats in healthy elderly were more frequent than among younger persons. Between 80 and 88 years of age, many functions crucial to the chances of living a rich and vital life were found deteriorating in the elderly persons. High age, male sex, dementia, congestive heart failure, and low values of S-creatinine were shown to be independent factors connected with an increased death risk.S. 1-112: sammanfattning, s. 115-177: 5 uppsatser
digitalisering@umu.se
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Santos, Talmir Augusto Faria Brisola dos. "Efeito da lesão eletrolítica do órgão subfornicial sobre os padrões cardiovasculares e salivatórios em ratos." Universidade de Taubaté, 2009. http://www.bdtd.unitau.br/tedesimplificado/tde_busca/arquivo.php?codArquivo=414.
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Hipótese do estudo: O presente trabalho foi desenvolvido a partir da hipótese de que existe relação entre o órgão subfornicial de ratos (OSF) e o fluxo, conteúdo protéico e eletrolítico da saliva, pressão arterial e frequência cardíaca. Proposição: Objetivo deste estudo foi investigar a possível relação entre o órgão subfornicial (OSF) e o fluxo, conteúdo proteico e eletrolítico da saliva, pressão arterial e frequência cardíaca em ratos. Metodologia: Para o desenvolvimento do estudo, foram utilizados vinte ratos machos adultos da raça Wistar (Rattus norvegicus var. albinus), pesando entre duzentos e cinquenta e trezentas gramas por meio de experimentação progressiva da fase 0 (F0) até a fase III (FIII); Os vinte animais passaram pela estimulação salivar (F0 e FI), depois pela canulação do OSF- lesão fictícia(FII) e posteriormente pela lesão eletrolítica (FIII), Com a lesão no OSF, permitiu averiguar a participação dos corpos celulares e fibras de passagem presentes na região reguladora da secreção salivar, fluxo salivar, conteúdo proteico e eletrolítico da saliva e as alterações nos padrões cardiovasculares. Para efetivação da lesão no OSF,(FIII) um eletrodo de aço inoxidável com 0.5mm de diâmetro de ponta, sem isolamento, foi introduzido na área do OSF pela cânula previamente posicionada na (FII). No quinto dia do período pós-operatório, o fluxo salivar foi estimulado com uso da pilocarpina injetada intraperitonealmente (IP) em todas as fases e os animais da (FI) usados como referência em relação aos lesionados fictícios e eletrolíticos considerados Fase III. Em todas as fases do experimento, determinou-se o conteúdo protéico e eletrolítico da saliva, e os padrões cardiovasculares e pressóricos. Resultados: Os resultados foram apresentados como média e erro padrão médio. Para avaliação da interação entre fatores (lesão/droga/efeito) foi utilizada o teste de Newman post-hoc (p<0,05). Foi evidenciada relação da lesão do OSF (FIII) com os padrões cardiovasculares, demonstrando efeito hipertensor da pilocarpina (FII) (326mmHg) em relação à (FIII) (94mmHg), aumento da Freqüência Cardíaca (F0 e FII) (31018bpm) e redução significativa da FC em (FIII) (23323bpm) (p<0,05) Com relação ao Fluxo salivar houve redução em (FIII) em relação à (FI e FII) (177 17mg/7min), (50124mg/7min) respectivamente. Conclusões: O OSF está intimamente relacionado com as alterações do fluxo salivar, secreção, conteúdo protéico e eletrolítico da saliva e alterações cardiovasculares em ratos.Hypothesis of the study: Hypothesis of the study: The present work was developed from the hypothesis that exists a relation enters the subfornical agency of rats (OSF) and the flow, protein and electrolyte content of the saliva, blood pressure and heart rate. Proposition: Objective of this study was to investigate the possible relation between the subfornical agency (OSF) and the flow, content protein and electrolyte of the saliva, blood pressure and heart rate in rats.Methodology: For the development of the study, (20) twenty adult male rats of the race Wistar (Rattus norvegicus var. albinus), weighing between two hundred and fifty and three hundred grams by means of gradual experimentation of phase 0 (F0) until the III phase (FIII); The twenty animals had passed for the salivary stimulation (F0 and FI), later for the cannulation of the OSF- fictitious injury (FII) and later for the electrolyte injury (FIII), With the injury in the OSF, allowed to inquire the participation of the cellular bodies and staple fibres present in the regulatory region of the salivary secretion , salivary flow , protein and electrolyte content of the saliva and the changes in the cardiovascular standards. For efficacy of the injury in the OSF, (FIII) a stainless steel electrode with 0.5mm of tip diameter, without isolation, was introduced in the area of the OSF for the cannula previously located in (FII). In the fifth day of the postoperative period, the salivary flow was stimulated with use of the pilocarpine injected intraperitoneally (IP) in all the phases and the animals of (FI) used ones as reference in relation to the fictitious and electrolyte injured ones considered Phase III. At all stages of the experiment, we determined the protein and electrolyte content of saliva, and blood pressure patterns and cardiovascular. Results: The results had been presented as average and error average standard. For evaluation of the interaction between factors (injury/drug/effect) the test of Newman was used posthoc (p< 0,05). Was evidenced relation of injury of OSF (FIII) with cardiovascular standards , demonstrating effect hypertensive of pilocarpine (FII) (32 (6mmHg) in relation to (FIII) (9 (4mmHg), increase of Heart Rate(F0 and FII) (310 (18bpm) and significant reduction of the FC in (FIII) (233 (23bpm) (p< 0,05) With regard to the salivary flow had reduction in (FIII) in relation to (FI and FII) (177 (17mg/7min), (501 (24mg/7min) respectively.Conclusions: The OSF is closely related to changes in salivary flow, secretion, protein and electrolyte content of saliva and cardiovascular changes in rats.
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Oliveira, Paulo Marcio de. "Avaliação de dose nos sistemas de irradiação de bolsas de sangue para esterilização." Universidade de São Paulo, 2014. http://www.teses.usp.br/teses/disponiveis/85/85131/tde-14112014-142426/.
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O procedimento de esterilização do sangue é adotado para que sejam mínimas as condições necessárias de manifestação da doença do enxertoversus- hospedeiro associada à transfusão de sangue (DECHT), síndrome, geralmente fatal. Neste trabalho foi utilizada a solução Fricke gel desenvolvida no IPEN para a obtenção de um objeto simulador de bolsa de sangue empregada no processo de irradiação para esterilização de sangue em um irradiador Gammacell Elan. O objetivo desse trabalho é determinar a homogeneidade da dose aplicada no processo de esterilização de bolsas de sangue. Foram utilizadas as técnicas de Espectrofotometria de Absorção Óptica (EAO), Densitometria Óptica (DO), Termoluminescência (TL) e Ressonância Magnética por Imagem (RMI) e os softwares Numaris® versão 4 (Syngo-MR-A30) e MatLab® (versão 9.0) para a aquisição e reconstrução das imagens. O estudo foi realizado posicionando no canister do irradiador inicialmente uma bolsa e, posteriormente, duas bolsas e três bolsas e aplicando uma dose nominal de 25 Gy. O fabricante do irradiador Gammacell Elan recomenda que seja irradiado em cada procedimento o valor máximo de três bolsas de sangue. Os resultados obtidos indicam que o irradiador Gammacell Elan não demostrou homogeneidade na distribuição da dose. Irradiando uma bolsa de sangue foi obtida uma diferença de dose de 3,65 %, enquanto para duas bolsas de sangue essa diferença caiu para 2,65 % e quando posicionadas três bolsas de sangue a diferença de dose foi de 1,88 %. O tempo de abertura de fechamento do equipamento de irradiação é responsável pela diferença de dose 3,17 % acima da dose prevista à realização das irradiações de bolsas de sangue. Os resultados obtidos usando o filme radiocrômico Grafchromic MD-55 de dupla face aplicada à avaliação de dose no sistema de rotação de irradiação não apresentou resultados reproduzíveis.The blood sterilization procedure is adopted so that the necessary conditions of manifestation of graft-versus-host syndrome, disease associated with blood transfusion (AT-GVHD), usually a fatal disease, are minimal. In this work the Fricke gel solution developed in IPEN was used for obtaining a phantom of blood bag used in the irradiation process for sterilization of blood in a Gammacell irradiator Elan. The objective of this study was to evaluate the homogeneity of the applied radiation sterilization dose in the blood bags. Optical Absorption Spectroscopy (OAS), Optical Densitometry (OD), Thermoluminescence (TL) and Magnetic Resonance Imaging (MRI) technics and Numaris® software version 4 (Syngo-MR-A30) and MatLab® (version 9.0) softwares were used to images acquisition and reconstruction. The study was conducted positioning one bag in the canister irradiator initially and subsequently two and three bags together and applying a nominal dose of 25 Gy. The Elan Gammacell irradiator manufacturer recommends that the maximum values of three blood bags are irradiated in each procedure. The results indicate that the Gammacell irradiator Elan did not presents homogeneous dose distribution. Radiating a blood bag a dose difference of 3.65 % was obtained, while for two bags of blood that difference dropped to 2.65% and when positioned three blood bags the difference in dose was 1.88 %. The opening and closing times of the irradiation source is responsible for an increasing in the planned irradiation dose of 3.17%. The results obtained using radiochromic double-sided Grafchromic MD-55 film applied to dose evaluation in the rotation irradiation system did not present reproducible results.
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Ellery, Paul E. R. "Expression and modulation of tissue factor and tissue factor pathway inhibitor in an endothelial cell based model." Curtin University of Technology, School of Biomedical Sciences, 2008. http://espace.library.curtin.edu.au:80/R/?func=dbin-jump-full&object_id=18719.
Full textAbstract:
Haemostasis is a complex physiological process involving cellular and plasma protein components that interact to keep the blood fluid under normal conditions and prevent blood loss after vessel injury by promoting clot formation. Primary haemostasis encompasses the activation and aggregation of platelets and is supported by secondary haemostasis, in which the coagulation factors of the plasma interact in a complex series of reactions. Secondary haemostasis is initiated by the exposure of tissue factor (TF) to the blood after vessel injury. TF forms a complex with activated factor VII (FVIIa), which in turn activates factor X (FXa) and ultimately results in fibrin formation. The TF-FVIIa complex and FXa are tightly regulated by tissue factor pathway inhibitor (TFPI), a trivalent Kunitz-type protease inhibitor. The endothelium, consisting of endothelial cells (ECs), constitutes the inner lining of all blood vessels. As such, it is in constant contact with the blood and plays a major role in haemostasis by synthesising and storing both pro- and anti- coagulant substances, including TF and TFPI. Release of TFPI from ECs is increased after exposure to both unfractionated and low molecular weight heparins, though the mechanisms are not clearly defined. TFPI circulates in plasma, predominantly bound to lipoproteins, though the effect of the three major lipoproteins [low density (LDL), very low density (VLDL) and high density (HDL)] on the release of TFPI from ECs is not well established. Furthermore, previous studies have not systematically investigated the effect of these lipoproteins on both TF and TFPI. The initial aim of this project was to establish assays for the measurement of TF activity and TFPI antigen to supplement the TFPI activity assay that is well established in our laboratory.These assays were then used to determine the effects of heparin and the major lipoproteins on the expression of TF and the release of TFPI on/from ECs. Human umbilical vein endothelial cells (HUVECs) were used as the EC model because their collection and isolation is well established and they have biochemical and physiological properties representative of in vivo conditions. A TF activity assay, based on a previously published method, was successfully modified and validated for the measurement of cell surface TF (standard curve R2 = 0.997). Despite exhaustive attempts, adaptation of this assay for plasma TF was unsuccessful, raising doubts regarding the plasma fractionation procedure of the originally published assay [Fukuda, C., Iijima, K. and Nakamura, K. (1989). "Measuring tissue factor (factor III) activity in plasma." Clinical Chemistry 35(9): 1897â€1900]. A novel insect cell expression system was used to produce well defined recombinant TFPI standards for use in TFPI activity and antigen assays. For the first time, truncated TFPI variants, containing the first Kunitz domain only, the first and second Kunitz domains only, and the first through third Kunitz domains minus the carboxyl terminus, were successfully produced in insect cells, though the full length molecule was not. Possible reasons for this include codon bias, protein instability and/or the signal peptide used. An ELISA to measure TFPI antigen was designed using a monoclonal antiâ€TFPI antibody directed against the Nâ€terminus for protein capture and a polyclonal anti†TFPI antibody for detection. The assay was successfully optimised (standard curve R2 = 0.978, intraâ€assay CV = 4.8%), however it produced inaccurate results (normal range = 498.7 ± 156.3 ng/mL), probably due to the antibody combination used.
TF and TFPI activity assays were used to determine the effect of both unfractionated and low molecular weight heparins (UFH and LMWH, respectively) on the release of TFPI and the expression of TF from/on ECs. A significant increase in the secretion of functional TFPI from ECs due to heparin (0 U/ml vs 1 and 10 U/mL) was demonstrated only in the presence of serum (UFH: 9.0 mU/mL vs 18.3 and 18.4 mU/mL, p < 0.0001; LMWH: 8.8 mU/mL vs 13.3 and 21.4 mU/mL, p < 0.05), suggesting, for the first time, that a component of serum is required for the heparinâ€dependent release of TFPI. The effect of LDL, VLDL and HDL on the release of TFPI and the expression of TF from/on ECs was also investigated. All three lipoprotein fractions increased the secretion of functional TFPI after one hour incubation (LDL: 12.5 μg/mL, p < 0.01; 25 μg/mL, p < 0.05; VLDL: 50 μg/mL, p < 0.01; HDL: 50 μg/mL, p < 0.05). This is the first data to demonstrate a HDLâ€dependent increase in released TFPI. After 24 hours, both LDL and VLDL decreased levels of secreted functional TFPI (LDL: 25 μg/mL, p < 0.01; 50 μg/mL, p < 0.01; VLDL: 12.5 μg/mL, p < 0.01), probably due to the oxidation and subsequent association of both lipoprotein species with TFPI. Surprisingly, both LDL and VLDL decreased cell surface TF, though this effect was not dose dependent. These results suggest that the major lipoproteins have a short term anticoagulant effect which is reversed in the longer term due to lipid oxidation. In summary, this thesis describes the successful adaptation of a chromogenic assay for the measurement of cell surface TF activity and the production of truncated TFPI variants.
Both will be used for the measurement of TF and TFPI, their association with thrombus formation and propagation, and investigations into potential therapeutic applications of TFPI. The results presented in this thesis extend the current knowledge on the expression and release of TF and TFPI on/from ECs by heparin, highlighting the importance of serum in the heparin dependent release of TFPI in vitro. Furthermore, it describes for the first time the effects of the major lipoprotein fractions on TFPI release and TF expression. The data support novel mechanisms by which LDL and VLDL are procoagulant, and HDL anticoagulant. This study provides a foundation for future research of the TF pathway in cellular models, which is critical in increasing the understanding of the pathogenesis and treatment of thrombotic disease. vitro. Furthermore, it describes for the first time the effects of the major lipoprotein fractions on TFPI release and TF expression. The data support novel mechanisms by which LDL and VLDL are procoagulant, and HDL anticoagulant. This study provides a foundation for future research of the TF pathway in cellular models, which is critical in increasing the understanding of the pathogenesis and treatment of thrombotic disease.
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Сухорукова, І. В. "Особливості публічного управління у сфері донорства крові та її компонентів." Master's thesis, Сумський державний університет, 2021. https://essuir.sumdu.edu.ua/handle/123456789/82875.
Full textAbstract:
У роботі здійснено теоретичний аналіз питання публічного управління у сфері донорства крові та її компонентів. Визначено поняття «публічне управління у сфері донорства крові та її компонентів». Висвітлено європейський досвід публічного управління в сфері донорства крові та її компонентів. Проаналізовано основні напрямки державної політики України у сфері публічного управління донорства крові та її компонентів. Представлено результати аналізу публічного управління ТОВ «Сумський обласний центр служби крові». Окреслено рекомендації, щодо вирішення проблем електронного документообігу у лікарні.В работе осуществлен теоретический анализ вопроса публичного управления в сфере донорства крови и ее компонентов. Определено понятие «публичное управление в сфере донорства крови и ее компонентов». Освещены европейский опыт публичного управления в сфере донорства крови и ее компонентов. Проанализированы основные направления государственной политики Украины в сфере публичного управления донорства крови и ее компонентов. Представлены результаты анализа публичного управления ООО «Сумской областной центр службы крови». Определены рекомендации по решению проблем электронного документооборота в больнице.
The paper provides a theoretical analysis of public administration in the field of blood donation and its components. The concept of "public administration in the field of blood donation and its components" is defined. The European experience of public administration in the field of blood donation and its components is highlighted. The main directions of the state policy of Ukraine in the field of public management of blood donation and its components are analyzed. The results of the analysis of the public administration of Sumy Regional Blood Service Center LLC are presented. Recommendations for solving the problems of electronic document management in the hospital are outlined.
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Perelman, Iris. "The Epidemiology of Multiple Blood Component Transfusion." Thesis, Université d'Ottawa / University of Ottawa, 2019. http://hdl.handle.net/10393/38867.
Full textAbstract:
Multicomponent transfusion, or the transfusion of two or more different blood products, has been poorly studied to date, as most of the existing literature has focused on the use of individual blood products. This is of concern as multicomponent transfusion recipients likely differ with respect to characteristics and health outcomes from patients transfused with only one type of blood component (e.g. greater illness severity). Consequently, available data on individual blood product use and outcomes may not be applicable to multicomponent transfused patients. This thesis project identified and synthesized existing literature on the epidemiology of multicomponent transfusion in hospital inpatients, as well as the characteristics and outcomes of its recipients. Based on 37 observational studies, we found that the prevalence of multicomponent transfusion varied greatly by patient population, transfusion timeframe, and type of multicomponent transfusion being studied. The most common types of multicomponent transfusion across the 37 studies were co-transfusions of red blood cells (RBCs) and platelets, and co-transfusions of RBCs and plasma. Multicomponent transfusion was found to be associated with several negative health outcomes, however this was based on low quality evidence due to lack of control for confounding by indication. Our systematic review on multicomponent transfusion identified several knowledge gaps, including the need for studies focusing on patients with hematological malignancies, and studies identifying patient characteristics predictive of multicomponent transfusion. To address areas of knowledge deficiency, and to characterize multicomponent transfusion locally at our own center, we designed and conducted a retrospective cohort study of adult, transfused hospital inpatients. Based on 55,719 transfused inpatient admissions at the Ottawa Hospital between 2007 and 2017, we calculated the overall prevalence of multicomponent transfusion to be 25.1% (95% CI: 24.7%, 25.5%). Similar to the findings of our systematic review, the prevalence varied greatly by patient type, transfusion timeframe, and type of multicomponent transfusion. In particular, in hematology patients, the prevalence of multicomponent transfusion was 51%. Other patient groups frequently receiving multicomponent transfusions at our institution were cardiac surgery, critical care, cardiology, vascular surgery, trauma, surgery, and internal medicine patients. Using multivariable regression analysis, we found that patient sex, age, and type were predictive of multicomponent transfusion requirement. Additionally, controlling for illness severity and burden, multicomponent transfusion was associated with increased odds of in-hospital mortality, institutional discharge compared to discharge home, and greater length of hospital stay compared to patients transfused with only RBCs. Given our findings that multicomponent transfusion recipients make up a large proportion of transfused hospital patients, and that they have poorer outcomes, it is of importance to continue characterizing these patients – and not only focus on patients receiving a single type of blood component – and to evaluate and monitor the appropriateness of multicomponent transfusion. Additionally, as transfusion practice and guidelines are known to vary from region to region, it is important to study multicomponent transfusion locally, as generalizing results from other studies and centers may not be appropriate. Obtaining robust information on multicomponent transfusion – including prevalence, predictors, and potential health consequences – can aid clinicians in their decision-making for patient blood management, potentially minimizing unnecessary patient exposure to blood products, and maximizing the use of transfusion alternatives and blood conservation methods.
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Biggin, Katherine J. "The epidemiology of blood component transfusion in eastern Scotland." Thesis, University of Edinburgh, 2008. http://hdl.handle.net/1842/29176.
Full textAbstract:
The cross-linked PhD study dataset comprises information on blood component use within a subset of Scottish hospitals that received approximately 48% of the red blood cell units delivered by the Scottish National Blood Transfusion Service in the year 2000. The dataset contains 41,431 transfusion day records of used units that represent 21,309 transfused patients and 60,130 used red blood cell units, 4,795 used platelet units, 9,446 used fresh frozen plasma units, and 1,7598 used cryoprecipitate units. For red blood cell, platelet and plasma use, few patients were intensely transfused with a large proportion of blood component units. 13,894 (23%) red blood cell units were attributed to 4,664 clinical events defined as red blood cell-using procedures; 7,286 (12%) red blood cell units were attributed to 726 patients with a diagnosis of haematological disease. The remaining 65% of red blood cell units were attributed to case groups which, though not fully classified, include patients with multiple transfusion day records and/or multiple SMR01/cancer/death records, patients with malignant neoplasms (other than those defined for haematological case group), patients with markers for gastrointestinal bleeding, and elderly patients. The data was also used to describe blood component use by age, and further, used to model the effect of the aging population on the demand for blood. Other potential applications of the study dataset relating to blood use modelling are also described. The results can be used for future planning and to inform guidelines and policy, with the aim of changing practice and promoting the appropriate use of blood.
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Irving, Gordon. "A survey of blood and blood component usage amongst South African anaesthetists in teaching hospital practice." Thesis, University of Cape Town, 1990. http://hdl.handle.net/11427/26279.
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Patelis, Antonios. "IgE sensitization against food allergens : Natural history, relation to airway inflammation and asthma." Doctoral thesis, Uppsala universitet, Institutionen för medicinska vetenskaper, 2015. http://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-251396.
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Background: According to recent studies in children, IgE sensitization not only against perennial allergens, but also against food allergens, is related to asthma risk and increased airway inflammation. During the last decade, a new technique for IgE determination based on allergen components has become available, but its use in epidemiological studies has been limited. Aims: To investigate the relationship between the pattern of IgE sensitization to allergen components and the prevalence of asthma, airway inflammation and hyperresponsiveness in a population-based setting. To examine the relationship of IgE sensitization to allergen extract, and airway inflammation, airway hyperresponsiveness and blood eosinophilia in asthmatics. To examine the natural history of IgE sensitization to food allergens in adults. To compare extract-based and component-based IgE measurements in relation with new-onset respiratory disease and airway inflammation and hyperresponsiveness. Methods: The present thesis is based on cross-sectional and longitudinal analyses of the adult, the population-based study ECRHS (European Community Health Survey) and a cross-sectional, observational study of young subjects with asthma. IgE sensitization was examined by means of both extract-based and component-based tests. Airway inflammation was assessed by exhaled NO and airway hyperresponsiveness with methacholine test. Results: IgE sensitization to food allergens independently related to increased airway inflammation in both a population-based study and a study of asthmatics. Furthermore, a relation was found with increased blood eosinophils in asthmatics. The decrease in prevalence of IgE sensitization against food allergens during a 9-year follow-up was larger than the decrease of aeroallergens. Subjects with IgE sensitization to both cat extract and components showed more frequent airway inflammation, greater bronchial responsiveness and higher likelihood of developing asthma and rhinitis than subjects with IgE sensitization only to cat extract. Conclusions: The presence of IgE antibodies against food allergens was independently associated with airway and systemic inflammation. Both aeroallergens and food allergens should be examined in order to understand the signaling of local and systemic inflammation in asthma. Prevalence of IgE sensitization to food decreased in adults to a larger extent than IgE sensitization against aeroallergens. Measurement of IgE sensitization to cat allergen components appears to have a higher clinical value than extract-based measurement
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Jones, Allison R. "OUTCOMES ASSOCIATED WITH BLOOD COMPONENT TRANSFUSION IN ADULT TRAUMA PATIENTS." UKnowledge, 2015. http://uknowledge.uky.edu/nursing_etds/14.
Full textAbstract:
The purpose of this dissertation was to evaluate outcomes associated with blood component (BC) transfusion in adult trauma patients. Specific aims were to: 1) explore the relationship between traumatic injury, hemorrhage, and BC transfusion, focusing on consequences of the component storage lesion through presentation of a conceptual model; 2) systematically review research literature comparing outcomes of massively transfused major trauma patients based on ratios of BCs received; 3) evaluating the relationship between type of blood transfusion trauma patients received (whole blood versus BCs) and mortality likelihood after controlling for demographic and clinical variables; 4) evaluating the relationship between volume and ratio of BCs transfused to trauma patients and development of inflammatory complications (ICs) after controlling for demographic and clinical variables.
Specific aim one was addressed through the development of a conceptual model, depicting the current state of knowledge regarding the storage lesion, and short-/long-term outcomes of traumatic injury, hemorrhage, and blood transfusion. The second specific aim was addressed through a systematic review of studies that grouped critically injured, massively transfused patients based on ratios of BCs they received, and compared clinical outcomes among groups. Findings from this analysis revealed increased survival likelihood with massive transfusion of BCs in a 1:1:1 (packed red blood cells [PRBCs], fresh frozen plasma [FFP], platelets [PLTs]) fashion. The third specific aim involved a secondary analysis of the National Trauma Data Bank to evaluate the relationship between type of transfusion trauma patients received (whole blood versus BCs) and mortality. Patients who received BCs experienced a higher mortality likelihood compared with those who received whole blood. The fourth specific aim was addressed through a secondary analysis of the Inflammation and Host Response to Injury Trauma-Related Data Base, to evaluate the relationship between volume and ratio of BCs transfused and development of ICs in patients with major trauma. Findings revealed that total transfused volume of PRBCs, injury severity, and comorbidities were associated with development of ICs. There were no differences in time to complication between PRBCs:FFP or PRBCs:PLTs ratio groups.
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Cardoso, Rafael Silva. "Avaliação do impacto hematológico na dinâmica do ferro em doadores de sangue submetidos à coleta automatizada de células- aférese, de duplo concentrado de hemácias do hemonúcleo de um hospital oncológico." Botucatu, 2016. http://hdl.handle.net/11449/144404.
Full textAbstract:
Orientador: Elenice DeffuneBanca: Rosana Rossi Ferreira
Banca: André Filadelpho
Resumo: Trata-se de um trabalho do tipo coorte para avaliar a espoliação dos depósitos de ferro em doadores de sangue. Sabendo-se que um percentual expressivo da população brasileira é portadora de ferro deficiência, e tendo em vista as inovações tecnológicas envolvendo os processos hemoterápicos este projeto teve como objetivo a avaliação da dinâmica de ferro com o monitoramento de parâmetros tais como: hematócrito, hemoglobina, dosagem de ferro e ferritina pré transfusionais e quatro meses após a doação, em quatro diferentes grupos de estudo:A,C,Ce D. Foi feita pelo teste ANOVA simples e para as variáveis sem distribuição normal pelo teste não paramétrico de Mann-Whitney e o teste de Kruskal Wallis, teste T-Pareado e Wilcoxon. No primeiro momento de análise (M1), com análise intra-grupos, as variações estatísticas foram presentes apenas nos parâmetros de Hb (p. 0,017), onde as variações estiveram presentes quando comparados os grupos A x D (p. 0,034) e C x D (p. 0,028) e Ht (p. <0,01) onde as variações estiveram presentes quando comparados os grupos A x D (p. 0,034) e C x D (p. 0,028). No segundo momento de análise (M2) foi identificada diferença entre os grupos, entretanto, devido à baixa significância estatística não foi possível identificar a diferença exata por grupo. Quando comparado entre os momentos um e dois- (M1 x M2) foi identificado redução da média de todos os parâmetros para os grupos A, B e C, significância estatística para o parâmetro de hemoglobina para todos os gru... (Resumo completo, clicar acesso eletrônico abaixo)
Abstract: It is a work of the cohort to evaluate the plundering of iron deposits in blood donors. Knowing that a significant percentage of the population is disabled iron, and in view of the technological innovations involving haemotherapic processes this project aimed to evaluate the iron dynamics with the monitoring of parameters such as hematocrit, hemoglobin, iron dosing and pre transfusion ferritin months after the donation, in four different study groups: A, B, C and D. Statistical analyses was made by ANOVA and simple test for variables without normal distribution using the nonparametric Mann-Whitney and Kruskal Wallis test, Paired t-test and Wilcoxon. At first analysis (M1) and intra-group analysis, statistical variations were present only in Hb parameters (p. 0.017), where variations were present when comparing the x groups D (p. 0.034) and C x D (p. 0028) and HT (p. <0.01) in which variations were present when comparing the groups D x (p. 0.034) and C x D (p. 0028). In the second stage of analysis (M2) was identified differences between the groups, however, due to the low statistical significance was not possible to identify the exact difference per group. When compared between one and two- moments (M1 and M2) was identified reduction in the average of all parameters for groups A, B and C for the statistical significance hemoglobin parameter for all groups and significance for the parameter Ferritin except for the B group, which is the only one that showed improvement in optimization of parameters except hemoglobin. Blood donation decreases hemoglobin levels in the short and medium term blood donors when compared in two stages with 4 months apart; for the indicator hematocrit and determination of serum iron there was a decrease of the indices was statistically significant only for the group A (individuals who had never donated before); as the suggestion in... (Complete abstract electronic access below)
Mestre
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Tynngård, Nahreen. "Free oscillation rheometry in the assessment of platelet quality /." Linköping : Linköping University, Department of Clinical and Experimental Medicine, 2008. http://urn.kb.se/resolve?urn=urn:nbn:se:liu:diva-11525.
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Kordzadeh, Ali. "Blood and blood component transfusion on 30-day mortality and morbidity of infra-renal ruptured abdominal aortic aneurysm." Thesis, Anglia Ruskin University, 2017. https://arro.anglia.ac.uk/id/eprint/702581/1/Kordzadeh_2017.pdf.
Full textAbstract:
This thesis for the first time in the literature, through a single cohort (n=82),
systematic review and meta-aggregation of the data, has identified that the
majority (>85%) of ruptured abdominal aortic aneurysm (rAAA) do not present
with coagulopathy. In addition, the thesis for the first time, through a retro and
prospective cohort study, has demonstrated that the hemostatic resuscitation
protocols derived from military and civilian trauma for the correction of
coagulopathy with a blood product ratio of one unit of packed red blood cell to
one unit of fresh frozen plasma to one pool of platelet (1:1:1), contributes to
increased postoperative (30-day) morbidity and mortality, especially thrombotic
complications. This was attributed to different baseline demographics,
pathophysiology and coagulation status. Through a comparative study, the
thesis then confirms that such transfusion practice not only contributes to
adverse outcomes, but also has no impact on final coagulation status of rAAAs.
In addition, through a retro and prospective cohort study, a novel hematological
marker (neutrophil to lymphocyte ratio) (NLR) was identified as an independent
predictor of morbidity in rAAAs. This thesis was set on the background of
significant research into all factors that could contribute to pathogenesis,
inhibition and progression of rAAAs.
This thesis concludes that the use of additional blood products (fresh frozen
plasma and platelet) in hemostatic resuscitation of ruptured abdominal aortic
aneurysms alongside packed red blood cell is not evidence based and a single
protocol derived from one cohort (military and civilian trauma) of patients does
not apply to another. The use of additional products in ruptured abdominal aortic
aneurysms should be tailored to the individual hematological and clinical
requirements and not as a part of a set transfusion ratio (1:1:1). This thesis has
resulted in a change of practice and has created a platform for further search of
the optimal transfusion protocol in this cohort of patients.
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Kordzadeh, Ali. "Blood and blood component transfusion on 30-day mortality and morbidity of infra-renal ruptured abdominal aortic aneurysm." Thesis, Anglia Ruskin University, 2017. http://arro.anglia.ac.uk/702581/.
Full textAbstract:
This thesis for the first time in the literature, through a single cohort (n=82), systematic review and meta-aggregation of the data, has identified that the majority (>85%) of ruptured abdominal aortic aneurysm (rAAA) do not present with coagulopathy. In addition, the thesis for the first time, through a retro and prospective cohort study, has demonstrated that the hemostatic resuscitation protocols derived from military and civilian trauma for the correction of coagulopathy with a blood product ratio of one unit of packed red blood cell to one unit of fresh frozen plasma to one pool of platelet (1:1:1), contributes to increased postoperative (30-day) morbidity and mortality, especially thrombotic complications. This was attributed to different baseline demographics, pathophysiology and coagulation status. Through a comparative study, the thesis then confirms that such transfusion practice not only contributes to adverse outcomes, but also has no impact on final coagulation status of rAAAs. In addition, through a retro and prospective cohort study, a novel hematological marker (neutrophil to lymphocyte ratio) (NLR) was identified as an independent predictor of morbidity in rAAAs. This thesis was set on the background of significant research into all factors that could contribute to pathogenesis, inhibition and progression of rAAAs. This thesis concludes that the use of additional blood products (fresh frozen plasma and platelet) in hemostatic resuscitation of ruptured abdominal aortic aneurysms alongside packed red blood cell is not evidence based and a single protocol derived from one cohort (military and civilian trauma) of patients does not apply to another. The use of additional products in ruptured abdominal aortic aneurysms should be tailored to the individual hematological and clinical requirements and not as a part of a set transfusion ratio (1:1:1). This thesis has resulted in a change of practice and has created a platform for further search of the optimal transfusion protocol in this cohort of patients.
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Sink, Carolyn A. "Canine Platelet Concentrates: An In Vitro Study to Effectively Provide a Source of Functional Platelets." Thesis, Virginia Tech, 2002. http://hdl.handle.net/10919/31620.
Full textAbstract:
This study monitored the storage lesion of 15 units of canine platelet concentrates harvested by differential centrifugation. Canine platelet concentrates were stored at 20-24° C in a platelet rotator for a total of 9 days; the storage lesion of three second generation platelet storage containers was compared. The battery of in vitro tests used to monitor the storage lesion were selected from previous studies performed with human platelet concentrates separated by differential centrifugation. Based on these tests, canine platelet concentrates exhibited a storage lesion similar to human platelet concentrates. Metabolic analytes demonstrated decreasing pH, carbon dioxide, bicarbonate and glucose concentrations concurrent with increasing oxygen and lactate dehydrogenase activity over the 9-day period. Platelet structural changes were monitored by mean platelet volume, which began to increase on Day-5. Platelet function appeared to be compromised, as indicated by aggregation studies using collagen and adenosine diphosphate as agonists. Product sterility was maintained.
There was no consensus of data supporting superior performance of one platelet storage container. This study indicates that canine platelet concentrates may be harvested by differential centrifugation of whole blood. In vitro studies utilizing three second-generation platelet storage bags support a previous study and concurs that canine platelet concentrates stored at 20-24° C using continuous agitation are viable for at least 5 days.Master of Science