To see the other types of publications on this topic, follow the link: Blood lipids – Analysis.
Journal articles on the topic 'Blood lipids – Analysis'
Create a spot-on reference in APA, MLA, Chicago, Harvard, and other styles
Consult the top 50 journal articles for your research on the topic 'Blood lipids – Analysis.'
Next to every source in the list of references, there is an 'Add to bibliography' button. Press on it, and we will generate automatically the bibliographic reference to the chosen work in the citation style you need: APA, MLA, Harvard, Chicago, Vancouver, etc.
You can also download the full text of the academic publication as pdf and read online its abstract whenever available in the metadata.
Browse journal articles on a wide variety of disciplines and organise your bibliography correctly.
1
Yan, Jingyi, Jin-Xiu Zhu, Nan Lu, Shanshan Gao, Jianfeng Ye, Chengzhi Yu, Minghui Yue, and Xuerui Tan. "Superior grey relational analysis on blood lipids and hematological parameters." Grey Systems: Theory and Application 9, no. 2 (April 1, 2019): 207–12. http://dx.doi.org/10.1108/gs-11-2018-0058.
Full textAbstract:
Purpose The purpose of this paper is to investigate the superior relationship between blood lipid- and cardiovascular disease (CVD)-related hematological parameters using superior grey relational analysis (GRA). Design/methodology/approach A total of 294 individuals who underwent simultaneous routine blood examination and blood lipid examination in the Physical Examination Center of the First Affiliated Hospital of Shantou University Medical College were included in this study. Superior GRA was performed to find out the superior factor in CVD-related hematological parameters and blood lipids. CVD-related hematological parameters included red blood cell distribution width, white cell count, and platelet count, platelet distribution width, mean platelet volume, as well as platelet crit. The indicators of blood lipids analyzed here consist of low-density lipoprotein, high-density lipoprotein, triglyceride and total cholesterol. Findings The results showed that all the grey relational degree of hematological parameters and blood lipids were over 0.8; the superior factor in hematological parameters was PLT, whereas TC was the superior factor in blood lipids. Practical implications Findings of this study suggested that hematological parameters are closely related to blood lipids and a potential role for hematological parameters in the prediction of dyslipidemia, which need further study; TC has the greatest influence on hematological parameters, whereas TG displays a minimal impact. Originality/value To the authors’ best knowledge, it was the first study to analyze the relationship between various CVD-related hematological parameters and blood lipids via superior GRA.
2
Oriisi, M. T., M. Giovanhini, R. Bellu, C. Galluzzo, C. Agostoni, R. Besana, and E. Riva. "59 FACTOR ANALYSIS OF CORD BLOOD LIPIDS." Pediatric Research 24, no. 2 (August 1988): 270. http://dx.doi.org/10.1203/00006450-198808000-00085.
Full text
3
Oostendorp, Marlies, Udo FH Engelke, Michèl AAP Willemsen, and Ron A. Wevers. "Diagnosing Inborn Errors of Lipid Metabolism with Proton Nuclear Magnetic Resonance Spectroscopy." Clinical Chemistry 52, no. 7 (July 1, 2006): 1395–405. http://dx.doi.org/10.1373/clinchem.2006.069112.
Full textAbstract:
Abstract Background: Many severe diseases are caused by defects in lipid metabolism. As a result, patients often accumulate unusual lipids in their blood and tissues, and proper identification of these lipids is essential for correct diagnosis. In this study, we investigated the potential use of proton nuclear magnetic resonance (1H-NMR) spectroscopy to simultaneously identify and quantify (un)usual lipids present in the blood of patients with different inborn errors of lipid metabolism. Methods: We extracted blood plasma or serum lipids in chloroform–methanol (2:1 by volume). After addition of the nonvolatile chemical shift and concentration reference compound octamethylcyclotetrasiloxane, we performed 1H-NMR measurements on a 500-MHz spectrometer. Assignments were based on the literature, computer simulations, and reference spectra of relevant authentic standards. Results: Spectra of normal plasma samples allowed the identification of 9 lipid species. We found good correlation between conventional methods and 1H-NMR for cholesterol and triglyceride concentrations. We also investigated 4 inborn errors of lipid metabolism (3 in sterol metabolism and 1 in fatty acid metabolism). NMR analysis led to a correct diagnosis for all 4 diseases, whereas the concentration of the diagnostic metabolite could be determined for 3. Conclusions: 1H-NMR spectroscopy of blood plasma or serum lipid extracts can be used to accurately identify and quantify lipids. The method can also identify unusual lipids in the blood of patients with inborn errors of lipid metabolism. This technique may therefore be applicable in clinical diagnosis and follow-up.
4
Bull, Caroline J., Carolina Bonilla, Jeff M. P. Holly, Claire M. Perks, Neil Davies, Philip Haycock, Oriana Hoi Yun Yu, et al. "Blood lipids and prostate cancer: a Mendelian randomization analysis." Cancer Medicine 5, no. 6 (March 19, 2016): 1125–36. http://dx.doi.org/10.1002/cam4.695.
Full text
5
Zhao, Hao, Xue-min Gao, Xinxin Cao, Lu Zhang, Dao-Bin Zhou, and Jian Li. "Characteristics of Serum Lipidome in Patients with POEMS Syndrome." Blood 134, Supplement_1 (November 13, 2019): 3082. http://dx.doi.org/10.1182/blood-2019-122239.
Full textAbstract:
OBJECTIVE: POEMS syndrome is a rare plasma cell dyscrasia. Most patients had shrinking buccal pad fat and weight loss at disease onset, indicating abnormal lipid metabolism. Manifestations of POEMS syndrome, such as the elevated serum level of vascular endothelial growth factor, may also be mediated by lipids. However, the characteristics of serum lipidome of patients with POEMS syndrome have not been described. Therefore, this study aims to describe serum lipidome characteristics in patients with POEMS syndrome. METHODS: Serum samples of 24 POEMS syndrome patients newly-diagnosed at Peking Union Medical College from September, 2017 to July, 2018 were collected at baseline and after at least one cycle of treatment. Serum was also collected from a group of 24 healthy participants who were age-, gender-, BMI-matched with patients. UPLC-ESI-QTOF/MS profiling was used to analyze the molecular profile of lipid-containing organic extract of serum samples in the 320-1000 Da range. Multivariate data analysis (PCA and PLS-DA) was used to identify altered lipids between matched serum samples. For altered lipids between patients and healthy controls, lipid related genes were identified with the human metabolome database. Lipid related genes overlapped with genes in transcription files specific to POEMS syndrome compared with normal plasma cells, MGUS and MM reported in a Japanese series were identified. RESULTS : POEMS syndrome patients had serum lipidome distinct from normal people, which is characterized by a decrease in most altered fatty acyls (82.6%, 43/52) and glycerolipids (81.8%, 18/22). (Figure A) The baseline serum 17-oxo-20Z-hexacosenoic acid level was independently associated with disease(OR 1.07, 95% CI 1.01-1.13, p = 0.021). A two-fatty-acyl lipid model could be constructed by leave-one-out cross validation based on peak intensity of 17-oxo-20Z-hexacosenoic acid andΩ-3 arachidonic acid to identify patients and healthy control with an accuracy of 100%. (Figure B) Comparing serum lipids pre- and post-treatment, 100% (14/14) altered fatty acyls and glycerolipids (3/3) increased after treatment, of which 50% fatty acyls and all glycerolipids overlapped with baseline decreased lipids compared with healthy control. (Figure C) In patients with complete or partial VEGF remission after treatment, sphingolipids AS 1-1, Cer(d18:1/17:0) and glycerophospholipid lysoPE(0:0/18:2(9Z,12Z)) was lower than in patient with poor VEGF remission. (Figure D) Of all 358 genes related with altered lipids between patients and healthy control, 6 genes were reported to be upregulated in CD138+ cells in the bone marrow of POEMS syndrome than in MGUS and 8 genes than in MM. MGLL, a gene with function of converting monoacylglycerides to free fatty acids and glycerol, was expressed higher in POEMS syndrome than in both MGUS and MM and may be related with decreased serum glycerolipids. PLA2G2D, a gene encodes a secreted member of the phospholipase A2 family, was expressed higher in POEMS syndrome than in MGUS and may be responsible for decreased lysolipids after treatment. CONCLUSION: POEMS syndrome patients had a distinct serum lipidome characteristic from healthy control. A two-lipids model could distinguish patients and healthy people with high accuracy. Altered lipids and lipids related genes indicate POEMS syndrome specific alteration in glycerolipids and lysolipids metabolism compared with MGUS and MM, providing a potential insight for further study of molecular mechanism of POEMS syndrome. Figure Disclosures No relevant conflicts of interest to declare.
6
Beaufrère, Hugues, Sara M. Gardhouse, R. Darren Wood, and Ken D. Stark. "The plasma lipidome of the Quaker parrot (Myiopsitta monachus)." PLOS ONE 15, no. 12 (December 1, 2020): e0240449. http://dx.doi.org/10.1371/journal.pone.0240449.
Full textAbstract:
Dyslipidemias and lipid-accumulation disorders are common in captive parrots, in particular in Quaker parrots. Currently available diagnostic tests only measure a fraction of blood lipids and have overall problematic cross-species applicability. Comprehensively analyzing lipids in the plasma of parrots is the first step to better understand their lipid metabolism in health and disease, as well as to explore new lipid biomarkers. The plasma lipidome of 12 Quaker parrots was investigated using UHPLC-MS/MS with both targeted and untargeted methods. Targeted methods on 6 replicates measured 432 lipids comprised of sterol, cholesterol ester, bile acid, fatty acid, acylcarnitine, glycerolipid, glycerophospholipid, and sphingolipid panels. For untargeted lipidomics, precursor ion mass-to-charge ratios were matched to corresponding lipids using the LIPIDMAPS structure database and LipidBlast at the sum composition or acyl species level of information. Sterol lipids and glycerophospholipids constituted the majority of plasma lipids on a molar basis. The most common lipids detected with the targeted methods included free cholesterol, CE(18:2), CE(20:4) for sterol lipids; PC(36:2), PC(34:2), PC(34:1) for glycerophospholipids; TG(52:3), TG(54:4), TG(54:5), TG(52:2) for glycerolipids; SM(d18:1/16:0) for sphingolipids; and palmitic acid for fatty acyls. Over a thousand different lipid species were detected by untargeted lipidomics. Sex differences in the plasma lipidome were observed using heatmaps, principal component analysis, and discriminant analysis. This report presents the first comprehensive database of plasma lipid species in psittacine birds and paves the way for further research into blood lipid diagnostics and the impact of diet, diseases, and drugs on the parrot plasma lipidome.
7
Le Faouder, Pauline, Julia Soullier, Marie Tremblay-Franco, Anthony Tournadre, Jean-François Martin, Yann Guitton, Caroline Carlé, Sylvie Caspar-Bauguil, Pierre-Damien Denechaud, and Justine Bertrand-Michel. "Untargeted Lipidomic Profiling of Dry Blood Spots Using SFC-HRMS." Metabolites 11, no. 5 (May 11, 2021): 305. http://dx.doi.org/10.3390/metabo11050305.
Full textAbstract:
Lipids are essential cellular constituents that have many critical roles in physiological functions. They are notably involved in energy storage and cell signaling as second messengers, and they are major constituents of cell membranes, including lipid rafts. As a consequence, they are implicated in a large number of heterogeneous diseases, such as cancer, diabetes, neurological disorders, and inherited metabolic diseases. Due to the high structural diversity and complexity of lipid species, the presence of isomeric and isobaric lipid species, and their occurrence at a large concentration scale, a complete lipidomic profiling of biological matrices remains challenging, especially in clinical contexts. Using supercritical fluid chromatography coupled with high-resolution mass spectrometry, we have developed and validated an untargeted lipidomic approach to the profiling of plasma and blood. Moreover, we have tested the technique using the Dry Blood Spot (DBS) method and found that it allows for the easy collection of blood for analysis. To develop the method, we performed the optimization of the separation and detection of lipid species on pure standards, reference human plasma (SRM1950), whole blood, and DBS. These analyses allowed an in-house lipid data bank to be built. Using the MS-Dial software, we developed an automatic process for the relative quantification of around 500 lipids species belonging to the 6 main classes of lipids (including phospholipids, sphingolipids, free fatty acids, sterols, and fatty acyl-carnitines). Then, we compared the method using the published data for SRM 1950 and a mouse blood sample, along with another sample of the same blood collected using the DBS method. In this study, we provided a method for blood lipidomic profiling that can be used for the easy sampling of dry blood spots.
8
Zou, Zhi-yong, Yi-de Yang, Shuo Wang, Bin Dong, Xiao-hui Li, and Jun Ma. "The importance of blood lipids in the association between BMI and blood pressure among Chinese overweight and obese children." British Journal of Nutrition 116, no. 1 (May 12, 2016): 45–51. http://dx.doi.org/10.1017/s0007114516001744.
Full textAbstract:
AbstractWe aimed to examine the contribution of blood lipids to the association between BMI and blood pressure (BP) in children with overweight and obesity. Data were collected in elementary and high schools of Chaoyang District, Beijing, China in 2012. Participants’ weight, height, BP and fasting plasma lipid profile were measured by standard protocols. Mediation analysis was used to examine the mediation role of blood lipids on the relation between BMI and BP, with age included as a covariate. We found that in boys 8·29 % (mediation effect=0·106, P=0·012) of the association between BMI and systolic BP was mediated through TAG. TAG mediated 12·53 % (mediation effect=0·093, P=0·018) and LDL-cholesterol mediated 7·75 % (mediation effect=0·57, P=0·046) of the association between BMI and diastolic BP was mediated by TAG and LDL-cholesterol, respectively. However, blood lipids did not show the mediation effect in girls. Our findings suggested that there was a sex difference in the contribution of blood lipids to the association between BMI and BP. Controlling TAG or LDL-cholesterol may be beneficial for reducing the risk of the BMI-related high BP in overweight boys; however, this outcome is not the case when controlling TAG or LDL-cholesterol in girls. This study may provide clues to explore the underlying mechanism of the association between obesity and hypertension.
9
Middelberg, Rita P., Nicholas G. Martin, and John B. Whitfield. "Longitudinal Genetic Analysis of Plasma Lipids." Twin Research and Human Genetics 9, no. 4 (August 1, 2006): 550–57. http://dx.doi.org/10.1375/twin.9.4.550.
Full textAbstract:
AbstractThe consensus from published studies is that plasma lipids are each influenced by genetic factors, and that this contributes to genetic variation in risk of cardiovascular disease. Heritability estimates for lipids and lipoproteins are in the range .48 to .87, when measured once per study participant. However, this ignores the confounding effects of biological variation measurement error and ageing, and a truer assessment of genetic effects on cardiovascular risk may be obtained from analysis of longitudinal twin or family data. We have analyzed information on plasma high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, and triglycerides, from 415 adult twins who provided blood on two to five occasions over 10 to 17 years. Multivariate modeling of genetic and environmental contributions to variation within and across occasions was used to assess the extent to which genetic and environmental factors have long-term effects on plasma lipids. Results indicated that more than one genetic factor influenced HDL and LDL components of cholesterol, and triglycerides over time in all studies. Nonshared environmental factors did not have significant long-term effects except for HDL. We conclude that when heritability of lipid risk factors is estimated on only one occasion, the existence of biological variation and measurement errors leads to underestimation of the importance of genetic factors as a cause of variation in long-term risk within the population. In addition our data suggest that different genes may affect the risk profile at different ages.
10
Thurgood, Lauren A., Lara Escane, Christie A. Bader, Karen M. Lower, Doug A. Brooks, and Bryone J. Kuss. "Chronic Lymphocytic Leukaemia Relies on Lipid Scavenging and Synthesis As an Energy Source." Blood 132, Supplement 1 (November 29, 2018): 3117. http://dx.doi.org/10.1182/blood-2018-99-120241.
Full textAbstract:
Abstract Dysregulation of cancer cell bioenergetics is one of the hallmarks of cancer. The Warburg effect is one such documented change. However, glucose metabolism is not universally increased in cancer cells. Uptake of radiolabelled glucose in chronic lymphocytic leukaemia (CLL) fails as a marker of proliferation and the underlying reason is not well elucidated [Conte et al, 2014]. Using proteomic and comprehensive lipid analyses, the preferred metabolic pathways of CLL cells have been identified. We have previously shown by proteomic analysis that circulating peripheral blood CLL cells demonstrate a significant increase in the expression of proteins pivotal in endogenous lipid synthesis pathways compared to B-cells from healthy individuals. These include fatty acid synthase (+3.84 fold), farnesyl diphosphate synthase (+2.17 fold), ATP-citrate lyase (+4.63 fold), citrate synthase (+2.45 fold) and acetyl-CoA acetyltransferase 1 (+5.84 fold). Conversely, analysis of the proliferation centres in CLL lymph nodes reveals increased expression of proteins involved in beta-oxidation, such as hydroxyacyl-CoA dehydrogenase (+2.02 fold). Additionally CLL cells shows a large increase in the levels of phospholipids found associated with lipid droplets compared to healthy B-cells, including phosphatidylinositol and phosphatidylethanolamine. Our current studies have expanded these observations in an attempt to determine if CLL cells have a preponderance for endogenous synthesis of lipids or exogenous uptake. We performed a comprehensive analysis of CLL cells from the bone marrow and peripheral blood using a variety of techniques. Transmission electron microscopy (TEM) images demonstrate striking morphological differences between normal B-cells and CLL cells with the inclusion of lipid droplets in the cytoplasm of CLL cells (Figure 1A) which was most evident in peripheral CLL samples. These were confirmed to be lipid droplets by the specific marker Bodipy 493/503 on flow cytometry (Fig. 1B) and confocal microscopy imaging with ReZolveL1 which targets neutral lipids (Fig. 1C). These showed a substantially higher level of lipid droplet staining in CLL cells compared to normal B-cells. Additionally there was higher expression of CD36 in CLL cells which is the receptor for exogenous lipid uptake into cells (Fig 1D). TEM analysis also revealed a high number of lysosomes in CLL cells, which was confirmed with lysotracker imaging on confocal microscopy (Fig. 1E). Lysosomes are now emerging as key players in lipid transport and biogenesis [Thelen and Zoncu, 2017]. Lipid droplets may be broken down by a process called autophagy mediated lipid degradation, or lipophagy, which relies on autophagosomes delivering lipid droplets to lysosomes. Autophagosomes were observed in several further bone marrow samples (Fig. 1F). The bone marrow samples also showed evidence of 'condensed mitochondria', indicative of high rates of beta-oxidation [Rossignol et al, 2004]. qPCR was then carried out on 90 genes involved in lipid metabolism. These results revealed significant differences between healthy B-cells and CLL cells. All CLL samples analysed demonstrated high expression of PLIN1, which protects lipid droplets from degradation, and low expression of APOC1, which prevents fatty acid uptake. From these observations we propose that peripheral CLL cells scavenge lipids from the periphery, mainly by uptake via the CD36 receptor, and increased endogenous lipid synthesis pathways. These results underpin a model for metabolism and enhanced survival for CLL cells in the hypoxic and potentially nutrient poor bone marrow/lymph node microenvironments. Peripheral CLL cells scavenge lipids; these excess lipids, which are toxic to the cell, are stored in lipid droplets which are protected from degradation by a high expression of PLIN proteins. Once these cells circulate back to the bone marrow and lymph nodes to proliferate, the lipid droplets are degraded, likely by lipophagy and neutral lipolysis which frees fatty acids to be used in beta oxidation in the mitochondria to sustain cell proliferation in the bone marrow and lymph node compartments. Our results begin to unravel CLL bioenergetics and dysregulation of cellular metabolism that occurs in this disease. We are now investigating whether the manipulation of these pathways, particularly lipophagy, may represent a novel therapeutic approach in CLL. Disclosures No relevant conflicts of interest to declare.
11
Bozorgi, Mahbubeh, Shekoufeh Nikfar, Mohammad Abdollahi, and Roja Rahimi. "Effect of pistachio on plasma lipids concentration: a meta-analysis of randomized controlled trials." Medicinal Plant Communications 4, no. 1 (January 30, 2021): 1–13. http://dx.doi.org/10.37360/mpc.21.4.1.01.
Full textAbstract:
Dyslipidemia and lipoprotein metabolism disorder are involved in pathogenesis of many important diseases such as diabetes, atherosclerosis, acute pancreatitis, and malignancies. The present study aimed to evaluate the effect of pistachio on plasma lipids. Electronic databases including Scopus, Pubmed, Science Direct, and Cochrane library were searched with the keywords “lipoprotein”, “blood lipid”, “dyslipidemia” or “hyperlipidemia” with “Pistachio” until June 2019. Two review authors independently checked eligibility and extracted data using a standard form. Information extracted included characteristics of the patients, dose of treatment, trial duration, quality score, and trial outcomes. Four randomized clinical trials with 213 subjects worked on the effect of pistachio on blood lipids were included. Comparison of pistachio rich diet with control yielded a significant effect size of -2.6 (95% CI:-4.4 –-0.7, p=0.006) for mean reduction in total cholesterol, a significant effect size of 5.1 (95% CI: 1.8 –8.3, p=0.002) for mean increase in HDL-cholesterol, a non-significant effect sizeof -0.3 (95% CI: -0.8 –0.3, p=0.4) for mean reduction in LDL-cholesterol and a non-significant effect size of -1.3 (95% CI: -4.4 –1.7, p=0.4) for mean reduction in triglyceride from baseline. The results demonstrated significant effect of pistachio on reducing total cholesterol and increasing HDL-cholesterol; however, its effect on lowering LDL-cholesterol and triglyceride was not significant. Further clinical trials are needed to confirm whether pistachio consumption for a certain period of time can significantly influence blood lipids.
12
A. H. Jasim, Mohamed. "Analysis of Blood Serum and Gallstone Lipids Composition of Gallstone Patients." JOURNAL OF EDUCATION AND SCIENCE 24, no. 1 (March 1, 2011): 1–12. http://dx.doi.org/10.33899/edusj.2011.51501.
Full text
13
Pan, An, Danxia Yu, Wendy Demark-Wahnefried, Oscar H. Franco, and Xu Lin. "Meta-analysis of the effects of flaxseed interventions on blood lipids." American Journal of Clinical Nutrition 90, no. 2 (June 10, 2009): 288–97. http://dx.doi.org/10.3945/ajcn.2009.27469.
Full text
14
Rohwedder, William K., Edward A. Emken, and Darhal J. Wolf. "Analysis of deuterium labeled blood lipids by chemical ionization mass spectrometry." Lipids 20, no. 5 (May 1985): 303–11. http://dx.doi.org/10.1007/bf02534263.
Full text
15
Proitsi, Petroula, Min Kim, Luke Whiley, Andrew Simmons, Martina Sattlecker, Latha Velayudhan, Michelle K. Lupton, et al. "Association of blood lipids with Alzheimer's disease: A comprehensive lipidomics analysis." Alzheimer's & Dementia 13, no. 2 (September 28, 2016): 140–51. http://dx.doi.org/10.1016/j.jalz.2016.08.003.
Full text
16
Guasch-Ferré, Marta, Jun Li, Frank B. Hu, Jordi Salas-Salvadó, and Deirdre K. Tobias. "Effects of walnut consumption on blood lipids and other cardiovascular risk factors: an updated meta-analysis and systematic review of controlled trials." American Journal of Clinical Nutrition 108, no. 1 (June 21, 2018): 174–87. http://dx.doi.org/10.1093/ajcn/nqy091.
Full textAbstract:
ABSTRACT BACKGROUND Intervention studies suggest that incorporating walnuts into the diet may improve blood lipids without promoting weight gain. OBJECTIVE We conducted a systematic review and meta-analysis of controlled trials evaluating the effects of walnut consumption on blood lipids and other cardiovascular risk factors. Design We conducted a comprehensive search of PubMed and EMBASE databases (from database inception to January 2018) of clinical trials comparing walnut-enriched diets with control diets. We performed random-effects meta-analyses comparing walnut-enriched and control diets for changes in pre-post intervention in blood lipids (mmol/L), apolipoproteins (mg/dL), body weight (kg), and blood pressure (mm Hg). RESULTS Twenty-six clinical trials with a total of 1059 participants were included. The following weighted mean differences (WMDs) in reductions were obtained for walnut-enriched diets compared with control groups: −6.99 mg/dL (95% CI: −9.39, −4.58 mg/dL; P < 0.001) (3.25% greater reduction) for total blood cholesterol (TC) and −5.51 mg/dL (95% CI: −7.72, −3.29 mg/dL; P < 0.001) (3.73% greater reduction) for low-density lipoprotein (LDL) cholesterol. Triglyceride concentrations were also reduced in walnut-enriched diets compared with control [WMD = −4.69 (95% CI: −8.93, −0.45); P = 0.03; 5.52% greater reduction]. More pronounced reductions in blood lipids were observed when walnut interventions were compared with American and Western diets [WMD for TC = −12.30 (95% CI: −23.17, −1.43) and for LDL = −8.28 (95% CI: −13.04, −3.51); P < 0.001]. Apolipoprotein B (mg/dL) was also reduced significantly more on walnut-enriched diets compared with control groups [WMD = −3.74 (95% CI: −6.51, −0.97); P = 0.008] and a trend towards a reduction was observed for apolipoprotein A [WMD = −2.91 (95% CI: −5.98, 0.08); P = 0.057]. Walnut-enriched diets did not lead to significant differences in weight change (kg) compared with control diets [WMD = −0.12 (95% CI: −2.12, 1.88); P = 0.90], systolic blood pressure (mm Hg) [WMD = −0.72 (95% CI: −2.75, 1.30); P = 0.48], or diastolic blood pressure (mm Hg) [WMD = −0.10 (95% CI: −1.49, 1.30); P = 0.88]. Conclusions Incorporating walnuts into the diet improved blood lipid profile without adversely affecting body weight or blood pressure.
17
Silliman, Christopher C., Lynn K. Boshkov, Zahra Mehdizadehkashi, David J. Elzi, William O. Dickey, Linda Podlosky, Gwen Clarke, and Daniel R. Ambruso. "Transfusion-related acute lung injury: epidemiology and a prospective analysis of etiologic factors." Blood 101, no. 2 (January 15, 2003): 454–62. http://dx.doi.org/10.1182/blood-2002-03-0958.
Full textAbstract:
Transfusion-related acute lung injury (TRALI) is a life-threatening complication of hemotherapy. We report a series of 90 TRALI reactions in 81 patients secondary to transfusion with whole blood platelets (72 reactions), apheresis platelets (2), packed red cells (15), and plasma (1). The overall prevalence was 1 in 1120 cellular components. To examine the epidemiology of TRALI, we completed a nested case-control study of the first 46 patients with TRALI compared with 225 controls who had received transfusions. We then completed a prospective analysis of possible biologic response modifiers responsible for 51 of the TRALI cases, including human leukocyte antigen (HLA) class I, class II, and granulocyte antibodies in donors and neutrophil (PMN) priming activity in the plasma of the implicated units and recipients. Two groups were at risk: patients with hematologic malignancies (P < .0004) and patients with cardiac disease (P < .0006). TRALI was associated with older platelets (P = .014). In the prospective study, antileukocyte antibodies were found in only 3.6% of cases. The implicated blood components had greater PMN priming activity than controls (P < .05), and compared with pretransfusion samples, TRALI patients' plasma demonstrated increases in both interleukin 6 (IL-6) and lipid (neutral lipids and lysophosphatidylcholines) priming activity (P < .05). We conclude that TRALI may be more frequent than previously recognized and that patient susceptibility, product age, and increased levels of bioactive lipids in components may predispose patients to TRALI. TRALI, like the acute respiratory distress syndrome, may be a 2-event phenomenon with both recipient predisposition and factors in the stored units playing major roles.
18
Lokhov, P. G., D. L. Maslov, E. E. Balashova, O. P. Trifonova, N. V. Medvedeva, T. I. Torkhovskaya, O. M. Ipatova, et al. "Mass spectrometry analysis of blood plasma lipidome as method of disease diagnostics, evuation of effectiveness and optimization of drug therapy." Biomeditsinskaya Khimiya 61, no. 1 (January 2015): 7–18. http://dx.doi.org/10.18097/pbmc20156101007.
Full textAbstract:
A new method for the analysis of blood lipid based on direct mass spectrometry of lipophilic low molecular weight fraction of blood plasma has been considered. Such technique allows quantification of hundreds of various types of lipids and this changes existing concepts on diagnostics of lipid disorders and related diseases. The versatility and quickness of the method significantly simplify its wide use. This method is applicable for diagnostics of atherosclerosis, diabetes, cancer and other diseases. Detalization of plasma lipid composition at the molecular level by means of mass spectrometry allows to assess the effectiveness of therapy and to optimize the drug treatment of cardiovascular diseases by phospholipid preparations.
19
Mertens, Evelien, Peter Clarys, Johan Lefevre, Ruben Charlier, Sara Knaeps, and Benedicte Deforche. "Longitudinal Study on the Association Between Cardiorespiratory Fitness, Anthropometric Parameters and Blood Lipids." Journal of Physical Activity and Health 13, no. 5 (May 2016): 467–73. http://dx.doi.org/10.1123/jpah.2015-0378.
Full textAbstract:
Background:Longitudinal evidence concerning the association between cardiorespiratory fitness (CRF) and blood lipids and between anthropometric parameters (ANTP) and blood lipids is limited. This study aimed to investigate the association between changes in CRF and ANTP and changes in blood lipids.Methods:In 2002–2004 and 2012–2014, 652 participants were tested. CRF was measured as VO2peak using a maximal ergometer test. Waist circumference (WC) and Body Mass Index (BMI) were used as ANTP. Blood samples were analyzed for total cholesterol (TC), HDL cholesterol, LDL cholesterol and triglycerides. A linear regression analysis was performed to investigate associations between changes in CRF and ANTP and changes in blood lipids.Results:After adjustment a decrease in CRF was associated with an increase in triglycerides and a decrease in HDL cholesterol in men. An increase in WC was associated with an increase in TC, LDL cholesterol and ratio total/HDL cholesterol and a decrease in HDL cholesterol, while an increase in BMI was associated with an increase in ratio total/HDL cholesterol and a decrease in HDL cholesterol.Conclusions:WC and BMI were more longitudinally associated with blood lipids compared with CRF. Improving ANTP can enhance the blood lipid profile, while CRF had only limited influence.
20
Pantasri, Tawiwan, Linda L. Wu, M. Louise Hull, Thomas R. Sullivan, Michael Barry, Robert J. Norman, and Rebecca L. Robker. "Distinct localisation of lipids in the ovarian follicular environment." Reproduction, Fertility and Development 27, no. 4 (2015): 593. http://dx.doi.org/10.1071/rd14321.
Full textAbstract:
Obesity is associated with decreased pregnancy rates due, in part, to compromised oocyte quality. The aim of the present cross-sectional study of 84 women undergoing oocyte aspiration was to: (1) compare insulin, lipids and glucose in follicular fluid with serum; (2) determine whether increased body mass index (BMI) and waist circumference, hyperinsulinaemia, dyslipidaemia or metabolic syndrome altered follicular fluid metabolites; and (3) determine relative lipid content in oocytes to reveal any influence of these parameters on oocyte quality and IVF outcomes. Insulin, glucose, triglyceride and free fatty acids were lower in follicular fluid than blood and not strictly correlated between compartments. Insulin, glucose and triglyceride positively correlated with increasing BMI and waist circumference in blood and follicular fluid. Insulin increased in follicular fluid in association with metabolic syndrome. Free fatty acid composition analysis showed saturated fatty acids, particularly palmitic and stearic acid, to be more prevalent in follicular fluid than blood. There were no associations between follicular fluid metabolites or oocyte lipid content and clinical outcomes; however, oocyte immaturity correlated with follicular fluid glucose and fatty acid levels, as well as metabolic syndrome. The present study confirms that the human ovarian follicular environment surrounding the oocyte exhibits a unique metabolite profile compared with blood, with distinct localisation of lipids within follicular fluid and oocytes.
21
Wannamethee, Goya, and A. Gerald Shaper. "Haematocrit: Relationships with Blood Lipids, Blood Pressure and other Cardiovascular Risk Factors." Thrombosis and Haemostasis 72, no. 01 (1994): 058–64. http://dx.doi.org/10.1055/s-0038-1648811.
Full textAbstract:
SummaryThe relationship between haematocrit and cardiovascular risk factors, particularly blood pressure and blood lipids, has been examined in detail in a large prospective study of 7735 middle-aged men drawn from general practices in 24 British towns. The analyses are restricted to the 5494 men free of any evidence of ischaemic heart disease at screening.Smoking, body mass index, physical activity, alcohol intake and lung function (FEV1) were factors strongly associated with haematocrit levels independent of each other. Age showed a significant but small independent association with haematocrit. Non-manual workers had slightly higher haematocrit levels than manual workers; this difference increased considerably and became significant after adjustment for the other risk factors. Diabetics showed significantly lower levels of haematocrit than non-diabetics. In the univariate analysis, haematocrit was significantly associated with total serum protein (r = 0*18), cholesterol (r = 0.16), triglyceride (r = 0.15), diastolic blood pressure (r = 0.17) and heart rate (r = 0.14); all at p <0.0001. A weaker but significant association was seen with systolic blood pressure (r = 0.09, p <0.001). These relationships remained significant even after adjustment for age, smoking, body mass index, physical activity, alcohol intake, lung function, presence of diabetes, social class and for each of the other biological variables; the relationship with systolic blood pressure was considerably weakened. No association was seen with blood glucose and HDL-cholesterol. This study has shown significant associations between several lifestyle characteristics and the haematocrit and supports the findings of a significant relationship between the haematocrit and blood lipids and blood pressure. It emphasises the role of the haematocrit in assessing the risk of ischaemic heart disease and stroke in individuals, and the need to take haematocrit levels into account in determining the importance of other cardiovascular risk factors.
22
Nawabi, Parwez, Athanasios Lykidis, Darder Ji, and Kasturi Haldar. "Neutral-Lipid Analysis Reveals Elevation of Acylglycerols and Lack of Cholesterol Esters in Plasmodium falciparum-Infected Erythrocytes." Eukaryotic Cell 2, no. 5 (October 2003): 1128–31. http://dx.doi.org/10.1128/ec.2.5.1128-1131.2003.
Full textAbstract:
ABSTRACT Here we show that blood-stage Plasmodium falciparum organisms accumulate a high mass of triacylglycerol and diacylglycerol. However, we failed to detect cholesterol esters, a second neutral lipid species reported to be important for a related apicomplexan, Toxoplasma gondii. Evidence for P. falciparum and T. gondii homologues of acyl coenzyme A:diacylglycerol acyltransferase suggests that acylglycerols may be the conserved neutral lipids in apicomplexans.
23
SERAFIM, Patricia Valeria Pereira, Adiel Goes de FIGUEIREDO JR, Aledson Vitor FELIPE, Edson Guimaraes Lo TURCO, Ismael Dale Cotrim Guerreiro da SILVA, and Nora Manoukian FORONES. "STUDY OF LIPID BIOMARKERS OF PATIENTS WITH POLYPS AND COLORECTAL CÂNCER." Arquivos de Gastroenterologia 56, no. 4 (October 2019): 399–404. http://dx.doi.org/10.1590/s0004-2803.201900000-80.
Full textAbstract:
ABSTRACT BACKGROUND: Colorectal cancer (CRC) is one of the leading causes of cancer worldwide. Early diagnostic methods using serum biomarkers are required. The study of omics, most recently lipidomics, has the purpose of analyzing lipids for a better understanding of human lipidoma. The evolution of mass spectrometry methods, such as MALDI-MS technology, has enabled the detection and identification of a wide variety of lipids with great potential to open new avenues for predictive and preventive medicine. OBJECTIVE: To determine the lipid profile of patients with colorectal cancer and polyps. METHODS: Patients with stage I-III CRC, adenomatous polyps and individuals with normal colonoscopy were selected. All patients underwent peripheral blood collection for lipid extraction. The samples were analyzed by MALDI-MS technique for lipid identification. STATISTICAL ANALYSIS: Univariate and multivariate (principal component analysis [PCA] and discriminant analysis by partial least squares [PLS-DA]) analyses workflows were applied to the dataset, using MetaboAnalyst 3.0 software. The ions were identified according to the class of lipids using the online database Lipid Maps (http://www.lipidmaps.org). RESULTS: We included 88 individuals, 40 with CRC, 12 with polyps and 32 controls. Boxplot analysis showed eight VIP ions in the three groups. Differences were observed between the cancer and control groups, as well as between cancer and polyp, but not between polyps and control. The polyketide (810.1) was the lipid represented in cancer and overrepresented in polyp and control. Among the patients with CRC we observed differences between lipids with lymph node invasion (N1-2) compared to those without lymph node invasion (N). CONCLUSION: Possible lipid biomarkers were identified among cancer patients compared to control and polyp groups. The polyketide lipid (810.1) was the best biomarker to differentiate the cancer group from control and polyp. We found no difference between the biomarkers in the polyp group in relation to the control.
24
Turner, Beate, Christian Mølgaard, and Peter Marckmann. "Effect of garlic (Allium sativum) powder tablets on serum lipids, blood pressure and arterial stiffness in normo-lipidaemic volunteers: a randomised, double-blind, placebo-controlled trial." British Journal of Nutrition 92, no. 4 (October 2004): 701–6. http://dx.doi.org/10.1079/bjn20041255.
Full textAbstract:
Recent studies have cast doubt on the proposed lipid-lowering and blood pressure-lowering effects of garlic. We tested the effect of dried garlic (Allium sativum) powder on blood lipids, blood pressure and arterial stiffness in a 12-week randomised, double-blind, placebo-controlled trial. Seventy-five healthy, normo-lipidaemic volunteers (men and women aged 40–60 years) were assigned to dried garlic powder tablets (10·8 mg alliin (3-(2-propenylsulfinyl)-l-alanine)/d, corresponding to about three garlic cloves) or placebo. Sixty-two subjects were eligible for the per-protocol analysis. The primary outcome measure was serum total cholesterol concentration. Secondary outcome measures were LDL-cholesterol, HDL-cholesterol and triacylglycerol concentrations, blood pressure and arterial stiffness (assessed by pulse wave velocity). No significant differences between the garlic and placebo groups were detected for any of the outcome measures. However, garlic powder was associated with a near-significant decrease (12 %) in triacylglycerol concentration (P=0·07). In conclusion, garlic powder tablets have no clinically relevant lipid-lowering and blood pressure-lowering effects in middle-aged, normo-lipidaemic individuals. The putative anti-atherosclerotic effect of garlic may be linked to risk markers other than blood lipids.
25
Peng, Bing, Sascha Geue, Cristina Coman, Patrick Münzer, Dominik Kopczynski, Canan Has, Nils Hoffmann, et al. "Identification of key lipids critical for platelet activation by comprehensive analysis of the platelet lipidome." Blood 132, no. 5 (August 2, 2018): e1-e12. http://dx.doi.org/10.1182/blood-2017-12-822890.
Full textAbstract:
Key Points First quantitative analysis of dynamic platelet lipidome modulation reveals key lipids altered in platelet activation. Lipidomics in a knockout approach unravel SMPD1 as a powerful modulator of platelet lipidome and activation via regulation of SPC.
26
Dattilo, A. M., and P. M. Kris-Etherton. "Effects of weight reduction on blood lipids and lipoproteins: a meta-analysis." American Journal of Clinical Nutrition 56, no. 2 (August 1, 1992): 320–28. http://dx.doi.org/10.1093/ajcn/56.2.320.
Full text
27
Clarke, R., C. Frost, R. Collins, P. Appleby, and R. Peto. "Dietary lipids and blood cholesterol: quantitative meta-analysis of metabolic ward studies." BMJ 314, no. 7074 (January 11, 1997): 112. http://dx.doi.org/10.1136/bmj.314.7074.112.
Full text
28
Arai, Ryo, Sayo Soda, Tomoko Okutomi, Hiroko Morita, Fumito Ohmi, Tomoe Funakoshi, Akihiro Takemasa, and Yoshiki Ishii. "Lipid Accumulation in Peripheral Blood Dendritic Cells and Anticancer Immunity in Patients with Lung Cancer." Journal of Immunology Research 2018 (2018): 1–8. http://dx.doi.org/10.1155/2018/5708239.
Full textAbstract:
We studied the subsets of peripheral blood dendritic cells (DCs) and lipid accumulation in DCs to investigate the involvement of DCs in the decreased anticancer immunity of advanced lung cancer patients. We analyzed the population of DC subsets in peripheral blood using flow cytometry. We then determined lipid accumulation in the DCs using BODIPY 650/665, a fluorophore with an affinity for lipids. Compared with healthy controls, the number of DCs in the peripheral blood of treatment-naive cancer patients was significantly reduced. In patients with stage III + IV disease, the numbers of myeloid DCs (mDCs) and plasmacytoid DCs were also significantly reduced. Lipid accumulation in DCs evaluated based on the fluorescence intensity of BODIPY 650/665 was significantly higher in stage III + IV lung cancer patients than in the controls. In the subset analysis, the fluorescence was highest for mDCs. The intracellularly accumulated lipids were identified as triglycerides. A decreased mixed leukocyte reaction was observed in the mDCs from lung cancer patients compared with those from controls. Taken together, the results show that lung cancer patients have a notably decreased number of peripheral blood DCs and their function as antigen-presenting cells is decreased due to their high intracellular lipid accumulation. Thereby, anticancer immunity is suppressed.
29
Martins, Tiago D., Joyce M. Annichino-Bizzacchi, Anna V. C. Romano, and Rubens Maciel Filho. "Principal Component Analysis on Recurrent Venous Thromboembolism." Clinical and Applied Thrombosis/Hemostasis 25 (January 1, 2019): 107602961989532. http://dx.doi.org/10.1177/1076029619895323.
Full textAbstract:
The rates of recurrent venous thromboembolism (RVTE) vary widely, and its causes still need to be elucidated. Statistical multivariate methods can be used to determine disease predictors and improve current methods for risk calculation. The objective of this study was to apply principal component analysis to a set of data containing clinical records of patients with previous venous thromboembolism and extract the main factors that predict recurrent thrombosis. Records of 39 factors including blood and lipid parameters, hereditary thrombophilia, antiphospholipid syndrome, clinical data regarding previous thrombosis and treatment, and Doppler ultrasound results were collected from 235 patients. The results showed that 13 principal components were associated with RVTE and that 18 of 39 factors are the important for the analysis. These factors include red blood cell, white blood cell, hematocrit, red cell distribution width, glucose, lipids, natural anticoagulant, creatinine, age, as well as first deep vein thrombosis data (distal/proximal, d-dimer, and time of anticoagulation). The results demonstrated that simple clinical parameters easy to be collected can be used to predict rates of recurrence and to develop new clinical decision support systems to predict the rates of RVTE.
30
Wang, Yue, and Xue Liang. "Correlation Analysis of Four Blood Coagulation Items with Blood Lipids and Blood Glucose in Stroke Patients with Type 2 Diabetes." OALib 07, no. 12 (2020): 1–5. http://dx.doi.org/10.4236/oalib.1107042.
Full text
31
Crouse, Stephen F., Barbara C. O’Brien, Peter W. Grandjean, Robert C. Lowe, J. James Rohack, John S. Green, and Homer Tolson. "Training intensity, blood lipids, and apolipoproteins in men with high cholesterol." Journal of Applied Physiology 82, no. 1 (January 1, 1997): 270–77. http://dx.doi.org/10.1152/jappl.1997.82.1.270.
Full textAbstract:
Crouse, Stephen F., Barbara C. O’Brien, Peter W. Grandjean, Robert C. Lowe, J. James Rohack, John S. Green, and Homer Tolson.Training intensity, blood lipids, and apolipoproteins in men with high cholesterol. J. Appl. Physiol.82(1): 270–277, 1997.—Twenty-six hypercholesterolemic men (mean cholesterol, 258 mg/dl; age, 47 yr; weight, 81.9 kg) completed 24 wk of cycle ergometer training (3 days/wk, 350 kcal/session) at either high ( n = 12) or moderate ( n = 14) intensity (80 and 50% maximal O2uptake, respectively, randomly assigned) to test the influence of training intensity on blood lipid and apolipoprotein (apo) concentrations. All physiological, lipid, and apo measurements were completed at 0, 8, 16, and 24 wk. Lipid data were analyzed via two × four repeated-measures analysis of variance (∝ = 0.0031). Training produced a significant decrease in body weight and increase in maximal O2uptake. No interactions between intensity and weeks of training were noted for any lipid or apo variable, and no between-group differences were significant before or throughout training. Therefore, intensity did not affect the training response. Regardless of intensity, apo AI and apo B fell 9 and 13%, respectively, by week 16 and remained lower through week 24( P < 0.0003). Total cholesterol fell transiently (−5.5%) by week 16( P < 0.0021) but returned to initial levels by week 24. Triglyceride, low-density-lipoprotein cholesterol, and high-density-lipoprotein (HDL) cholesterol did not change with training. In contrast, HDL2cholesterol rose 79% above initial levels by week 8 and 82% above initial levels by week 24( P < 0.0018); HDL3cholesterol fell 8 and 13% over the same training intervals ( P< 0.0026). These data show that changes in blood lipid and apo concentrations that accompany training in hypercholesterolemic men are not influenced by exercise intensity when caloric expenditure is held constant.
32
Kulminski, Alexander M., Yury Loika, Alireza Nazarian, and Irina Culminskaya. "Quantitative and Qualitative Role of Antagonistic Heterogeneity in Genetics of Blood Lipids." Journals of Gerontology: Series A 75, no. 10 (September 30, 2019): 1811–19. http://dx.doi.org/10.1093/gerona/glz225.
Full textAbstract:
Abstract Prevailing strategies in genome-wide association studies (GWAS) mostly rely on principles of medical genetics emphasizing one gene, one function, one phenotype concept. Here, we performed GWAS of blood lipids leveraging a new systemic concept emphasizing complexity of genetic predisposition to such phenotypes. We focused on total cholesterol, low- and high-density lipoprotein cholesterols, and triglycerides available for 29,902 individuals of European ancestry from seven independent studies, men and women combined. To implement the new concept, we leveraged the inherent heterogeneity in genetic predisposition to such complex phenotypes and emphasized a new counter intuitive phenomenon of antagonistic genetic heterogeneity, which is characterized by misalignment of the directions of genetic effects and the phenotype correlation. This analysis identified 37 loci associated with blood lipids but only one locus, FBXO33, was not reported in previous top GWAS. We, however, found strong effect of antagonistic heterogeneity that leaded to profound (quantitative and qualitative) changes in the associations with blood lipids in most, 25 of 37 or 68%, loci. These changes suggested new roles for some genes, which functions were considered as well established such as GCKR, SIK3 (APOA1 locus), LIPC, LIPG, among the others. The antagonistic heterogeneity highlighted a new class of genetic associations emphasizing beneficial and adverse trade-offs in predisposition to lipids. Our results argue that rigorous analyses dissecting heterogeneity in genetic predisposition to complex traits such as lipids beyond those implemented in current GWAS are required to facilitate translation of genetic discoveries into health care.
33
Radwan, Basseem, Adriana Adamczyk, Szymon Tott, Krzysztof Czamara, Katarzyna Kaminska, Ewelina Matuszyk, and Malgorzata Baranska. "Labeled vs. Label-Free Raman Imaging of Lipids in Endothelial Cells of Various Origins." Molecules 25, no. 23 (December 6, 2020): 5752. http://dx.doi.org/10.3390/molecules25235752.
Full textAbstract:
Endothelial cells (EC) constitute a single layer of the lining of blood vessels and play an important role in maintaining cardiovascular homeostasis. Endothelial dysfunction has been recognized as a primary or secondary cause of many diseases and it manifests itself, among others, by increased lipid content or a change in the lipid composition in the EC. Therefore, the analysis of cellular lipids is crucial to understand the mechanisms of disease development. Tumor necrosis factor alpha (TNF-α)-induced inflammation of EC alters the lipid content of cells, which can be detected by Raman spectroscopy. By default, lipid detection is carried out in a label-free manner, and these compounds are recognized based on their spectral profile characteristics. We consider (3S,3′S)-astaxanthin (AXT), a natural dye with a characteristic resonance spectrum, as a new Raman probe for the detection of lipids in the EC of various vascular beds, i.e., the aorta, brain and heart. AXT colocalizes with lipids in cells, enabling imaging of lipid-rich cellular components in a time-dependent manner using laser power 10 times lower than that commonly used to measure biological samples. The results show that AXT can be used to study lipids distribution in EC at various locations, suggesting its use as a universal probe for studying cellular lipids using Raman spectroscopy. The use of labeled Raman imaging of lipids in the EC of various organs could contribute to their easier identification and to a better understanding of the development and progression of various vascular diseases, and it could also potentially improve their diagnosis and treatment.
34
Arfuso, Francesca, Claudia Giannetto, Maria Francesca Panzera, Francesco Fazio, and Giuseppe Piccione. "Uncoupling Protein-1 (UCP1) in the Adult Horse: Correlations with Body Weight, Rectal Temperature and Lipid Profile." Animals 11, no. 6 (June 20, 2021): 1836. http://dx.doi.org/10.3390/ani11061836.
Full textAbstract:
This study aimed to evaluate the possible relationship among UCP1, body weight, rectal temperature and lipid profile in the horse. Thirty clinically healthy Italian Saddle geldings (6–10 years old) were enrolled after the informed owners’ consent. All horses were blood sampled and their body weight and rectal temperatures were recorded. On the sera obtained after blood centrifugation the concentration of UCP1, total lipids, phospholipids, non-esterified fatty acids (NEFAs), triglycerides, total cholesterol, high density lipoproteins (HDLs), low density lipoproteins (LDLs) and very low density lipoprotein fraction (VLDLs) was evaluated. Pearson’s correlation analysis was applied to assess the possible relationship between serum UCP1 concentration and the values of body weight, rectal temperature and lipid parameters. Serum UCP1 concentration showed no correlation with body weight, rectal temperature, HDLs and LDLs values, whereas it correlated negatively with serum total lipids, phospholipids, NEFAs, total cholesterol, triglycerides and VLDLs values (p < 0.0001). The findings suggest that in the adult horse the role of UCP1 is linked to the lipid metabolism rather than to thermoregulation.
35
Gao, Yanan, Lei Yu, Xiaohan Li, Chen Yang, Aiwen Wang, and Huiming Huang. "The Effect of Different Traditional Chinese Exercises on Blood Lipid in Middle-Aged and Elderly Individuals: A Systematic Review and Network Meta-Analysis." Life 11, no. 7 (July 19, 2021): 714. http://dx.doi.org/10.3390/life11070714.
Full textAbstract:
Although the impact of physical exercise on blood lipids is well documented, less information is available regarding the effect of traditional Chinese exercises (TCEs), and it is unclear what the best TCE treatment for dyslipidemia in middle-aged and elderly individuals is. The aim of this study was to systematically assess the effects of TCEs (Taijiquan, TJQ; Wuqinxi, WQX; Baduanjin, BDJ; Liuzijue, LZJ; Yijinjing, YJJ; Dawu, DW) on blood lipids in middle-aged and elderly individuals. Chinese and English databases were searched, including PubMed, China National Knowledge Infrastructure, Wanfang Database, Chongqing VIP, and Web of Science. A total of 42 randomized controlled trials (RCTs) including 2977 subjects were analyzed. Outcome indicators include total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triacylglyceride (TAG), and high-density lipoprotein cholesterol (HDL-C). Summary mean differences (MD) were calculated using pairwise and network meta-analysis with a random-effects model. The results of this study showed that compared to non-exercise intervention (NEI), all six kinds of TCE treatment had some kind of influence on blood lipid indicators, among which WQX and TJQ could improve all four blood lipid indicators, whereas BDJ was effective on three indicators but not on TC. The results of cumulative probability ranking showed that WQX (84.9%, 73.8%, 63.4%, 63.1% to TC, TAG, HDL-C, LDL-C, respectively) was at the top spot being the best intervention, followed by BDJ (55.6%, 83.7%, 68.4%, 56.1%) and TJQ (73.7%, 47.6%, 63.1%, 54.1%). The network meta-analysis of RCTs demonstrates that WQX may be the best TCE treatment for dyslipidemia in middle-aged and elderly individuals.
36
Hewald, Sandra, Katharina Josephs, and Michael Bölker. "Genetic Analysis of Biosurfactant Production in Ustilago maydis." Applied and Environmental Microbiology 71, no. 6 (June 2005): 3033–40. http://dx.doi.org/10.1128/aem.71.6.3033-3040.2005.
Full textAbstract:
ABSTRACT The dimorphic basidiomycete Ustilago maydis produces large amounts of surface-active compounds under conditions of nitrogen starvation. These biosurfactants consist of derivatives of two classes of amphipathic glycolipids. Ustilagic acids are cellobiose lipids in which the disaccharide is O-glycosidically linked to 15,16-dihydroxyhexadecanoic acid. Ustilipids are mannosylerythritol lipids derived from acylated β-d-mannopyranosyl-d-erythritol. Whereas the chemical structure of these biosurfactants has been determined, the genetic basis for their biosynthesis and regulation is largely unknown. Here we report the first identification of two genes, emt1 and cyp1, that are essential for the production of fungal extracellular glycolipids. emt1 is required for mannosylerythritol lipid production and codes for a protein with similarity to prokaryotic glycosyltransferases involved in the biosynthesis of macrolide antibiotics. We suggest that Emt1 catalyzes the synthesis of mannosyl-d-erythritol by transfer of GDP-mannose. Deletion of the gene cyp1 resulted in complete loss of ustilagic acid production. Cyp1 encodes a cytochrome P450 monooxygenase which is highly related to a family of plant fatty acid hydroxylases. Therefore we assume that Cyp1 is directly involved in the biosynthesis of the unusual 15,16-dihydroxyhexadecanoic acid. We could show that mannosylerythritol lipid production is responsible for hemolytic activity on blood agar, whereas ustilagic acid secretion is required for long-range pheromone recognition. The mutants described here allow for the first time a genetic analysis of glycolipid production in fungi.
37
Weise, Shelly D., Peter W. Grandjean, J. James Rohack, John W. Womack, and Stephen F. Crouse. "Acute changes in blood lipids and enzymes in postmenopausal women after exercise." Journal of Applied Physiology 99, no. 2 (August 2005): 609–15. http://dx.doi.org/10.1152/japplphysiol.01354.2004.
Full textAbstract:
The effectiveness of lifestyle intervention strategies to improve blood lipids in women may be dependent on preexisting cholesterol concentrations. We characterized the effects of cholesterol status on blood lipid, lipoprotein lipid, and lipid regulatory enzyme responses to a single session of aerobic exercise in physically active, postmenopausal women. In this study, blood samples were obtained from 12 women with high cholesterol (HC; ≥200 mg/dl) and 13 women with normal cholesterol (NC; <200 mg/dl), 24 h before (Pre), immediately after (IPE), and 24 and 48 h after an exercise session (treadmill walking at 70% peak oxygen consumption, 400 kcal). We found that repeated-measures analysis revealed the following: 1) preexercise cholesterol differences did not influence the lipid or lipoprotein lipid responses to exercise; 2) for both groups, triglyceride was significantly reduced (−8.5%) after exercise; 3) the concentration profile over time for high-density lipoprotein cholesterol was significant for both groups, first falling at IPE then rising back to Pre levels by 24 h after exercise; 4) the lecithin-cholesterol acyltransferase activity (LCATA) exercise response was group dependent, increasing modestly in the NC group at 24 and 48 h; 5) lipoprotein lipase activity (LPLA) increased at IPE (by 17%) in the HC group only and then fell at 24 and 48 h (by 21%) compared with Pre; and 6) cholesterol ester transfer protein activity was unchanged by exercise. From these findings, we conclude that in postmenopausal women, a single session of endurance exercise elicited a short-term, favorable decrease in triglycerides independent of initial blood cholesterol concentrations. However, LCATA and LPLA postexercise changes were influenced by preexercise cholesterol status.
38
Aslibekyan, Stella, Mark O. Goodarzi, Alexis C. Frazier-Wood, Xiaofei Yan, Marguerite R. Irvin, Eric Kim, Hemant K. Tiwari, et al. "Variants Identified in a GWAS Meta-Analysis for Blood Lipids Are Associated with the Lipid Response to Fenofibrate." PLoS ONE 7, no. 10 (October 31, 2012): e48663. http://dx.doi.org/10.1371/journal.pone.0048663.
Full text
39
Bubnova, Marina G., and Lev E. Parnes. "Modern principles of atherogenic dyslipidemia management in special groups of patients." CardioSomatics 11, no. 1 (September 26, 2020): 6–15. http://dx.doi.org/10.26442/22217185.2020.1.200089.
Full textAbstract:
Aim.To provide modern view on atherogenic disorders in the blood lipid spectrum, the principles of lipid-lowering therapy prescription in certain groups of patients, the safety issues of statin therapy in patients with chronic liver diseases.
Materials and methods.The data of 55 scientific sources published in the Russian and foreign press in 19882020 were analyzed.
Results.It is known that atherogenic dyslipidemia is a key factor in pathogenesis of atherosclerotic cardiovascular diseases. Various disorders in blood lipids and lipoproteins are identified. In this regard, there are certain recommendations for the analysis of lipids and lipoproteins in certain situations. The issues of interpreting extreme deviations from normal values of the blood lipid spectrum are discussed. Patient groups with different levels of the cardiovascular risk, which determines prescription of lipid-lowering therapy, are presented. Statins are the first line of lipid-lowering therapy for both the correction of dyslipidemia and the prevention of cardiovascular complications. The article discusses the tactics of prescribing statins in special groups of patients, their safety issues. It considers promising ways for increasing statins tolerability in patients with chronic liver diseases, primarily with non-alcoholic fatty liver disease, by adding of combined hepatoprotector.
Conclusions.Generally, modern tactics of statins prescription are based on dyslipidemia characteristics and the patients cardiovascular risk level. Moreover, statins are clinically effective in special patient groups and have a good tolerability profile.
40
Kruisbrink, Marlot, Wendy Robertson, Chen Ji, Michelle A. Miller, Johanna M. Geleijnse, and Francesco P. Cappuccio. "Association of sleep duration and quality with blood lipids: a systematic review and meta-analysis of prospective studies." BMJ Open 7, no. 12 (December 2017): e018585. http://dx.doi.org/10.1136/bmjopen-2017-018585.
Full textAbstract:
ObjectivesTo assess the longitudinal evidence of the relationships between sleep disturbances (of quantity and quality) and dyslipidaemia in the general population and to quantify such relationships.SettingSystematic review and meta-analysis following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines.MethodsWe performed a systematic search of PubMed and Embase (up to 9 September 2017), complemented with manual searches, of prospective population studies describing the association between sleep duration and quality and the incidence of dyslipidaemias. Relative risks (95% CIs) were extracted and pooled using a random effects model. Subgroup analyses by lipid type were performed. Heterogeneity and publication bias were also assessed. Quality was assessed with Downs and Black score.ParticipantsStudies were included if they were prospective, had measured sleep quantity and/or quality at baseline and either incident cases of dyslipidaemia or changes in blood lipid fractions assessed prospectively.Primary outcome measuresIncidence of dyslipidaemia and changes in lipid fractions. Dyslipidaemia was defined as a high total cholesterol, triglycerides, low-density lipoprotein cholesterol or low high-density lipoprotein cholesterol compared with the reference group.ResultsThirteen studies were identified (eight using sleep duration, four sleep quality and one both). There was heterogeneity in the sleep quality aspects and types of lipids assessed. Classification of sleep duration (per hour/groups) also varied widely. In the pooled analysis of sleep duration (6 studies, 16 cohort samples; 30 033 participants; follow-up 2.6–10 years), short sleep was associated with a risk of 1.01 (95% CI 0.93 to 1.10) of developing dyslipidaemia, with moderate heterogeneity (I2=56%, P=0.003) and publication bias (P=0.035). Long sleep was associated with a risk of 0.98 (95% CI 0.87 to 1.10) for dyslipidaemia, with heterogeneity (I2=63%, P<0.001) and no significant publication bias (P=0.248).ConclusionThe present analysis was unable to find supportive evidence of a significant relationship between sleep duration and the development of dyslipidaemia. However, heterogeneity and small number of studies limit the interpretation.PROSPERO registration numberCRD42016045242.
41
Farias, Paige, and Kathleen Melanson. "Cross Sectional Analysis of the Effect of Yogurt and Fiber Consumption on BMI, Blood Pressure, and Lipids in College Students." Current Developments in Nutrition 4, Supplement_2 (May 29, 2020): 750. http://dx.doi.org/10.1093/cdn/nzaa052_019.
Full textAbstract:
Abstract Objectives Results from recent studies suggest that maintaining a healthy gut microbiome is important for predicting health outcomes using biomarkers such as BMI, blood pressure, glucose, and lipids. College-aged students are an important population to consider as they are at a crucial stage in developing eating habits, including consumption of probiotic-rich, fermented foods and prebiotic fermentable nutrients. We hypothesize that yogurt consumption and fiber consumption will beneficially impact these outcomes and we explored a possible interaction. Methods In a cross-sectional design, 497 college students (76% female; 19.5 ± 3.62 yr; BMI 23.94 ± 4.72 kg/m2) enrolled in a general nutrition course completed the Dietary History Questionnaire II. Height and weight were measured, along with blood pressure with an electronic sphygmomanometer. Fasting blood glucose and lipids were measured with Cholestech. Median splits were used for yogurt (.05 cups/day) and fibers (18.71 grams/day) intakes. Analysis of Variance (ANOVA) was used to examine relationships of yogurt consumption and fiber consumption separately with BMI, blood pressure, and blood lipids. ANCOVA was used to control for added sugars intakes. To test for interactions between yogurt and fibers, 2 × 2 ANOVA and ANCOVA were used. Data are expressed as means ± standard deviations. Results Of the 497 students, 48% reported lower yogurt consumption while 50% reported lower fiber consumption. Univariate-measures analysis indicated a significant effect of higher yogurt consumption on BMI (P = .037), blood glucose (P = .048), and diastolic blood pressure (P = .035) while higher fiber consumption showed a significant effect on total cholesterol (P = .011), HDL (P = .045), and triglycerides (P = .006). LDL was not significantly impacted (P = .069). No significance differences were reported within interactions (P > .05). Conclusions Higher yogurt consumption was associated with lower BMI, blood glucose, and diastolic blood pressure, while higher fiber consumption was associated with beneficial effects on lipids. Lack of interaction between yogurt and fibers may be related to a low yogurt intake in this population. These findings may promote further research focusing on synbiosis to examine the impact of fiber when consumed conjunctively with probiotic foods. Funding Sources There was no external funding for this study.
42
Shih, Ming-Kuei, You-Lin Tain, Yu-Wei Chen, Wei-Hsuan Hsu, Yao-Tsung Yeh, Sam K. C. Chang, Jin-Xian Liao, and Chih-Yao Hou. "Resveratrol Butyrate Esters Inhibit Obesity Caused by Perinatal Exposure to Bisphenol A in Female Offspring Rats." Molecules 26, no. 13 (June 30, 2021): 4010. http://dx.doi.org/10.3390/molecules26134010.
Full textAbstract:
Resveratrol butyrate esters (RBE) are derivatives of resveratrol (RSV) and butyric acid and exhibit biological activity similar to that of RSV but with higher bioavailability. The aim of this study was designed as an animal experiment to explore the effects of RBE on the serum biochemistry, and fat deposits in the offspring rats exposed to bisphenol A (BPA), along with the growth and decline of gut microbiota. We constructed an animal model of perinatal Bisphenol A (BPA) exposure to observe the effects of RBE supplementation on obesity, blood lipids, and intestinal microbiota in female offspring rats. Perinatal exposure to BPA led to weight gain, lipid accumulation, high levels of blood lipids, and deterioration of intestinal microbiota in female offspring rats. RBE supplementation reduced the weight gain and lipid accumulation caused by BPA, optimised the levels of blood lipids, significantly reduced the Firmicutes/Bacteroidetes (F/B) ratio, and increased and decreased the abundance of S24-7 and Lactobacillus, respectively. The analysis of faecal short-chain fatty acid (SCFA) levels revealed that BPA exposure increased the faecal concentration of acetate, which could be reduced via RBE supplementation. However, the faecal concentrations of propionate and butyrate were not only significantly lower than that of acetate, but also did not significantly change in response to BPA exposure or RBE supplementation. Hence, RBE can suppress BPA-induced obesity in female offspring rats, and it demonstrates excellent modulatory activity on intestinal microbiota, with potential applications in perinatological research.
43
Martinelli, Nicola, Domenico Girelli, Barbara Lunghi, Mirko Pinotti, Giovanna Marchetti, Giovanni Malerba, Pier Franco Pignatti, Roberto Corrocher, Oliviero Olivieri, and Francesco Bernardi. "Polymorphisms at LDLR locus may be associated with coronary artery disease through modulation of coagulation factor VIII activity and independently from lipid profile." Blood 116, no. 25 (December 16, 2010): 5688–97. http://dx.doi.org/10.1182/blood-2010-03-277079.
Full textAbstract:
Abstract High levels of coagulation factor VIII (FVIII) have been associated with cardiovascular disease. Low-density lipoprotein receptor (LDLR) has been recently demonstrated to contribute to FVIII clearance from plasma. The aim of this study was to evaluate 3 single nucleotide polymorphisms in SMARCA4-LDLR gene locus (rs1122608, rs2228671, and rs688) and FVIII coagulant activity (FVIII:c) in subjects with (n = 692) or without (n = 291) angiographically confirmed coronary artery disease (CAD). High FVIII:c levels were an independent risk factor for CAD. The rs688 and rs2228671 genotypes were predictors of FVIII:c with T alleles associated with higher FVIII:c levels. The rs2228671T allele was associated also with reduced total and LDL-cholesterol levels. With respect to the risk of CAD, no association was found for rs2228671. Consistently with higher FVIII:c levels, the rs688T allele was associated with CAD, whereas, consistently with a favorable lipid profile, the rs1122608T allele was associated with a decreased CAD prevalence. After adjustment for classic cardiovascular risk factors, including plasma lipids, rs688 remained associated with CAD (OR for T carriers: 1.67 with 95% confidence interval, 1.10-2.54). Haplotype analysis confirmed such results. Our data suggest that polymorphisms at LDLR locus modulate FVIII:c levels and may be associated with CAD risk independently from plasma lipids.
44
Choy, Patrick C., Yaw L. Siow, David Mymin, and Karmin O. "Lipids and atherosclerosis." Biochemistry and Cell Biology 82, no. 1 (February 1, 2004): 212–24. http://dx.doi.org/10.1139/o03-085.
Full textAbstract:
Atherosclerosis is the leading cause of death in North America and within the next two decades will be the leading cause worldwide. Atherosclerosis is characterized by vascular obstruction from the deposits of plaque, resulting in reduced blood flow. Plaque rupture and the consequent thrombosis may lead to sudden blockage of the arteries and cause heart attack. High serum lipid levels, especially the elevated level of low-density lipoprotein (LDL), have been shown to be strongly related to the development of atherosclerosis. It is generally accepted that atherosclerotic lesions are initiated via an enhancement of LDL uptake by monocytes and macrophages. In the liver, uptake of plasma LDL is mediated via specific LDL receptors, but a scavenger receptor system is employed by macrophages. Plasma LDL must be modified prior to uptake by macrophages. Analysis of the lipid content in the oxidatively modified LDL from hyper lipidemic patients revealed that the level of lysophosphatidylcholine was greatly elevated, and the high level of the lysolipid was shown to impair the endothelium-dependent relaxation of the blood vessels. In a separate study, we showed that a high level of homocysteine caused the increase in cholesterol production and apolipoprotein B-100 secretion in hepatic cells. Statins have been used effectively to control the production of cholesterol in the liver, and recently, ezetimibe has been shown to supplement the efficacy of statins by inhibiting cholesterol absorption. The factor of elevated levels of triglyceride-rich lipoproteins in association with depressed high-density lipoproteins, usually in the context of insulin resistance, is an important contributor to atherosclerosis and can be effectively treated with fibric acid derivatives. In hyperhomocysteinemia, folic acid supplements may have a role in the control of cholesterol by reducing the plasma homocysteine level.Key words: atherosclerosis, low density lipoprotein (LDL), homocysteine, statin, folate.
45
Grellier, P., D. Rigomier, V. Clavey, J. C. Fruchart, and J. Schrevel. "Lipid traffic between high density lipoproteins and Plasmodium falciparum-infected red blood cells." Journal of Cell Biology 112, no. 2 (January 15, 1991): 267–77. http://dx.doi.org/10.1083/jcb.112.2.267.
Full textAbstract:
Several intraerythrocytic growth cycles of Plasmodium falciparum could be achieved in vitro using a serum free medium supplemented only with a human high density lipoprotein (HDL) fraction (d = 1.063-1.210). The parasitemia obtained was similar to that in standard culture medium containing human serum. The parasite development was incomplete with the low density lipoprotein (LDL) fraction and did not occur with the VLDL fraction. The lipid traffic from HDL to the infected erythrocytes was demonstrated by pulse labeling experiments using HDL loaded with either fluorescent NBD-phosphatidylcholine (NBD-PC) or radioactive [3H]palmitoyl-PC. At 37 degrees C, the lipid probes rapidly accumulated in the infected cells. After incubation in HDL medium containing labeled PC, a subsequent incubation in medium with either an excess of native HDL or 20% human serum induced the disappearance of the label from the erythrocyte plasma membrane but not from the intraerythrocytic parasite. Internalization of lipids did not occur at 4 degrees C. The mechanism involved a unidirectional flux of lipids but no endocytosis. The absence of labeling of P. falciparum, with HDL previously [125I]iodinated on their apolipoproteins or with antibodies against the apolipoproteins AI and AII by immunofluorescence and immunoblotting, confirmed that no endocytosis of the HDL was involved. A possible pathway of lipid transport could be a membrane flux since fluorescence videomicroscopy showed numerous organelles labeled with NBD-PC moving between the erythrocyte and the parasitophorous membranes. TLC analysis showed that a partial conversion of the PC to phosphatidylethanolamine was observed in P. falciparum-infected red cells after pulse with [3H]palmitoyl-PC-HDL. The intensity of the lipid traffic was stage dependent with a maximum at the trophozoite and young schizont stages (38th h of the erythrocyte life cycle). We conclude that the HDL fraction appears to be a major lipid source for Plasmodium growth.
46
Zhang, Tengfei, Si Chen, and Atushi Saito. "A META-ANALYSIS OF THE EFFECTS OF GREEN TEA COMBINED WITH PHYSICAL ACTIVITY ON BLOOD LIPIDS IN HUMANS." Revista Brasileira de Medicina do Esporte 26, no. 5 (October 2020): 454–60. http://dx.doi.org/10.1590/1517-869220202605212295.
Full textAbstract:
ABSTRACT Introduction: Most studies of green tea extract (GTE) combined with physical activity have reported a preventative effect for cardiovascular disease; however, the findings regarding the effects on serum lipids are controversial. Objective: This meta-analysis aimed to examine the evidence of the effects of GTE combined with physical activity on the serum lipid content in humans. Methods: In June 2017, we conducted electronic searches of PubMed, Web of Science, and Cochrane Library to identify pertinent studies: those with an experiment period exceeding two weeks, human randomized controlled trials (RCTs), and those that only assessed GTE with physical activity were included. A random effects model meta-analysis was used in this review. Results: A total of 271 citations were retrieved in our search of the electronic literature, and 7 RCTs, which included 608 individuals, were identified. Overall, there was no significant decrease in low-density lipoprotein cholesterol (LDL-C) (SMD:-0.169; 95% confidence interval [CI]:-0.414 to 0.076; I2=22.7%; p=0.177) or total cholesterol (TC) levels between the GTE and placebo combined with the physical activity group. Similar results were also observed for high density-lipoprotein cholesterol (HDL-C) and triglycerides (TG). In the subgroup and sensitivity analyses of the five studies, the TC levels of the subjects who received a lower dose of epigallocatechin gallate (EGCG) together with performing physical activity were significantly decreased. Conclusion: Current evidence suggests that green tea combined with physical activity does not improve the lipid and lipoprotein levels in humans. Level of evidence I; Systematic review.
47
de Groot, Rosa, Katja van den Hurk, Linda J. Schoonmade, Wim L. A. M. de Kort, Johannes Brug, and Jeroen Lakerveld. "Urban-rural differences in the association between blood lipids and characteristics of the built environment: a systematic review and meta-analysis." BMJ Global Health 4, no. 1 (January 2019): e001017. http://dx.doi.org/10.1136/bmjgh-2018-001017.
Full textAbstract:
IntroductionThe built environment defines opportunities for healthy eating and physical activity and may thus be related to blood lipids. The aim of this study is to systematically analyse the scientific evidence on associations between built-environment characteristics and blood lipid levels in adults.MethodsPubMed, EMBASE and Web of Science were searched for peer-reviewed papers on population-based studies up to 9 October 2017. We included studies that reported on built-environment characteristics and blood lipid levels in adult populations (≥18 years). Two reviewers independently screened titles/abstracts and full-texts of papers and appraised the risk of bias of included studies using an adapted version of the Quality Assessment Tool for Quantitative Studies. We performed meta-analyses when five or more studies had sufficient homogeneity in determinant and outcome.ResultsAfter screening 6902 titles/abstracts and 141 potentially relevant full-text articles, we included 50 studies. Forty-seven studies explored associations between urban versus rural areas with blood lipid levels. Meta-analyses on urban versus rural areas included 133 966 subjects from 36 studies in total. Total cholesterol levels were significantly and consistently higher in urban areas as compared with rural areas (mean difference 0.37 mmol/L, 95% CI 0.27 to 0.48). Urban/rural differences in high density lipoprotein cholesterol were inconsistent across studies and the pooled estimate showed no difference (0.00 mmol/L 95% CI −0.03 to 0.04). Low density lipoprotein (LDL) cholesterol and triglyceride levels were higher in urban than in rural areas (mean difference 0.28, 95% CI 0.17 to 0.39 and 0.09, 95% CI 0.03 to 0.14, respectively).ConclusionsTotal and LDL cholesterol levels and triglycerides were consistently higher in residents of urban areas than those of rural areas. These results indicate that residents of urban areas generally have less favourable lipid profiles as compared with residents of rural areas.Prospero registration numberCRD42016043226.
48
Notararigo, S., S. Bravo, M. Martin-Pastor, I. Baston-Rey, A. Quiroga-Castiñeira, J. E. Dominguez-Munoz, E. Domínguez Medina, and M. Barreiro-de Acosta. "P078 Proteomics and Lipidomics Analysis Revealed Alterations of Complement Activation and Lipid Metabolism in peripheral blood mononuclear cells of Inflammatory Bowel Disease Patients." Journal of Crohn's and Colitis 15, Supplement_1 (May 1, 2021): S180—S181. http://dx.doi.org/10.1093/ecco-jcc/jjab076.207.
Full textAbstract:
Abstract Background The lack of interaction between immune system cells and microbiota is considered a key driver of the pathogenesis of inflammatory bowel disease (IBD). The association of age-associated B cells (ABC) from antigen-experienced B cells and autoimmunity is now well established. The increased demands in lipid metabolism during immune response and cell activation upon autoimmune signals leads to lipid tag modifications of proteins. In IBD patients, we observed an impairment of B cell development, identifying a transitional B cell subset expressing CD38Hi, CD24Hi and CD19+. We hypothesize that metabolic alterations of proteins and lipids during immune cell activation might be a relevant mechanism of autoimmune disease. Methods An IBD cohort of patients in clinical remission under anti-TNF treatment was included in order to test metabolic and proteomic changes in peripheral blood mononuclear cells (PBMCs). Proteomic analysis was performed by Mass Spectrometry using a label free quantitative method (SWATH-MS analysis) and lead to identify a number of proteins underregulated (< 0.6) and overexpressed (> 1.5) fold change respect to control. Lipidomics of serum samples analyzed by proton Nuclear Magnetic Resonance spectroscopy (NMR)2 and multivariant statistics. Results Serum samples were obtained in Crohn disease (CD) n = 18, ulcerative colitis (UC) n = 9, before biological infusion and healthy controls (CTRL) n = 10. Proteins related with lipid metabolism like APOC2 for CD and APOB, APOA1 for UC, were differentially modulated. Significant differences in non-polar metabolites and lipids that phenotypically define CD and UC groups respect to CTRL were observed (Fig. 1) Fig. 1 Orthogonal-Partial Least Square-Discriminant Analysis (OPLS-DA) of the global NMR data of the serum samples of groups CD and CTRL. Indeed, proteins associated with complement activation like Complement Factor 1 and Complement C1q subcomponent subunit B were differentially expressed in CD group. These results were confirmed by lipidomic analysis, in which palmitic (Fig. 2) as well as other candidate lipids were more abundant in IBD groups than CTRL. Fig 2. Differences of palmitic acid concentration in CD and UC patients vs CTRL (Bonferroni test showed statistical difference between CTRL vs. CD and CTRL vs. UC with p = 0.02 and p = 0.004 respectively). Conclusion Our study detected high levels of proteins regulating lipid metabolism and palmitic in IBD patients. This observation can be interpreted as part of deleterious alterations of proteins through palmitoylation during B-cell activation and might be a relevant mechanism of disease. Further exploration of palmitate catabolism might shed light to the mechanisms that result in immunometabolic disorders.
49
Panth, Nisha, Kylie A. Abbott, Cintia B. Dias, Katie Wynne, and Manohar L. Garg. "Differential effects of medium- and long-chain saturated fatty acids on blood lipid profile: a systematic review and meta-analysis." American Journal of Clinical Nutrition 108, no. 4 (September 18, 2018): 675–87. http://dx.doi.org/10.1093/ajcn/nqy167.
Full textAbstract:
Abstract Background Medium-chain saturated fatty acids (MCFAs) may affect circulating lipids and lipoproteins differently than long-chain saturated fatty acids (LCSFAs), but the results from human intervention trials have been equivocal. Objective The aim of this study was to determine whether MCFAs and LCSFAs have differential impacts on blood lipids and lipoproteins. Design Five databases were searched (EMBASE, MEDLINE, CINAHL, Cochrane, and Scopus) until April 2018, and published clinical trials investigating the differential effects of dietary MCFAs and LCSFAs on blood lipids were included. Searches were limited to the English language and to studies with adults aged >18 y. Where possible, studies were pooled for meta-analysis using RevMan 5.2. The principle summary measure was the mean difference between groups calculated using the random-effects model. Results Eleven eligible crossover and 1 parallel trial were identified with a total of 299 participants [weighted mean ± SD age: 38 ± 3 y; weighted mean ± SD body mass index (kg/m2): 24 ± 2]. All studies were pooled for the meta-analysis. Diets enriched with MCFAs led to significantly higher high-density lipoprotein (HDL) cholesterol concentrations than diets enriched with LCSFAs (0.11 mmol/L; 95% CI: 0.07, 0.15 mmol/L) with no effect on triglyceride, low-density lipoprotein (LDL) cholesterol, and total cholesterol concentrations. Consumption of diets rich in MCFAs significantly increased apolipoprotein A-I (apoA-I) concentrations compared with diets rich in LCSFAs (0.08 g/L; 95% CI: 0.02, 0.14 g/L). There was no evidence of statistical heterogeneity for HDL cholesterol, apoA-I, and triglyceride concentrations; however, significant heterogeneity was observed for the total cholesterol (I2 = 49%) and LDL cholesterol analysis (I2 = 58%). Conclusion The findings of this research demonstrate a differential effect of MCFAs and LCSFAs on HDL cholesterol concentrations. Further investigations are warranted to elucidate the mechanism by which the lipid profile is altered. This trial was registered at www.crd.york.ac.uk/PROSPERO as CRD42017078277.
50
Markevych, Iana, Marie Standl, Dorothea Sugiri, Carla Harris, Werner Maier, Dietrich Berdel, and Joachim Heinrich. "Residential greenness and blood lipids in children: A longitudinal analysis in GINIplus and LISAplus." Environmental Research 151 (November 2016): 168–73. http://dx.doi.org/10.1016/j.envres.2016.07.037.
Full text