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Relevant bibliographies by topics / Blood lipoproteins – Analysis

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Academic literature on the topic 'Blood lipoproteins – Analysis'

Author: Grafiati

Published: 4 June 2021

Last updated: 11 February 2022

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Journal articles on the topic "Blood lipoproteins – Analysis"

1

Trentalance, A., G. Bruscalupi, L. Conti Devirgiliis, S. Leoni, M. T. Mangiantini, L. Rossini, S. Spagnuolo, and S. K. Erickson. "Changes in lipoprotein binding and uptake by hepatocytes during rat liver regeneration." Bioscience Reports 9, no. 2 (April 1, 1989): 231–41. http://dx.doi.org/10.1007/bf01116000.

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The binding and uptake of cholesterol enriched lipoproteins by isolated hepatocytes was decreased at 16 hours after partial hepatectomy, with a tendency to return to control values as the regeneration proceeds. The number of lipoprotein binding sites of total cellular membranes remained similar to control at 16 and 24 hours. The plasma lipoprotein pattern, determined by electrophoretic analysis, showed a lower per cent of very low density lipoproteins (VLDL) and a higher per cent of low density lipoproteins (LDL) at 16 and 24 hours post-partial hepatectomy. At these times, plasma lecithin: cholesterol acyltransferase (LCAT) activity was decreased. It is intriguing to suggest that the regenerating liver could regulated the blood lipoprotein pattern and the uptake of lipoproteins by modulating the surface expression of the receptors.

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Rose, Jeffrey R., Maureen A. Mullarkey, William J. Christ, Lynn D. Hawkins, Melvyn Lynn, Yoshito Kishi, Kishor M. Wasan, Kathy Peteherych, and Daniel P. Rossignol. "Consequences of Interaction of a Lipophilic Endotoxin Antagonist with Plasma Lipoproteins." Antimicrobial Agents and Chemotherapy 44, no. 3 (March 1, 2000): 504–10. http://dx.doi.org/10.1128/aac.44.3.504-510.2000.

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ABSTRACT E5531, a novel synthetic lipid A analogue, antagonizes the toxic effects of lipopolysaccharide, making it a potential intravenously administered therapeutic agent for the treatment of sepsis. This report describes the distribution of E5531 in human blood and its activity when it is associated with different lipoprotein subclasses. After in vitro incubation of [14C]E5531 with blood, the great majority (>92%) of material was found in the plasma fraction. Analysis by size-exclusion and affinity chromatographies and density gradient centrifugation indicates that [14C]E5531 binds to lipoproteins, primarily high-density lipoproteins (HDLs), with distribution into low-density lipoproteins (LDLs) and very low density lipoproteins (VLDLs) being dependent on the plasma LDL or VLDL cholesterol concentration. Similar results were also seen in a limited study of [14C]E5531 administration to human volunteers. The potency of E5531 in freshly drawn human blood directly correlates to increasing LDL cholesterol levels. Finally, preincubation of E5531 with plasma or purified lipoproteins indicated that binding to HDL resulted in a time-dependent loss of drug activity. This loss in activity was not observed with drug binding to LDLs or to VLDLs or chylomicrons. Taken together, these results indicate that E5531 binds to plasma lipoproteins, making its long-term antagonistic potency dependent on the plasma lipoprotein composition.

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Ohkawa, Ryunosuke, Hann Low, Nigora Mukhamedova, Ying Fu, Shao-Jui Lai, Mai Sasaoka, Ayuko Hara, et al. "Cholesterol transport between red blood cells and lipoproteins contributes to cholesterol metabolism in blood." Journal of Lipid Research 61, no. 12 (September 9, 2020): 1577–88. http://dx.doi.org/10.1194/jlr.ra120000635.

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Lipoproteins play a key role in transport of cholesterol to and from tissues. Recent studies have also demonstrated that red blood cells (RBCs), which carry large quantities of free cholesterol in their membrane, play an important role in reverse cholesterol transport. However, the exact role of RBCs in systemic cholesterol metabolism is poorly understood. RBCs were incubated with autologous plasma or isolated lipoproteins resulting in a significant net amount of cholesterol moved from RBCs to HDL, while cholesterol from LDL moved in the opposite direction. Furthermore, the bi-directional cholesterol transport between RBCs and plasma lipoproteins was saturable and temperature-, energy-, and time-dependent, consistent with an active process. We did not find LDLR, ABCG1, or scavenger receptor class B type 1 in RBCs but found a substantial amount of ABCA1 mRNA and protein. However, specific cholesterol efflux from RBCs to isolated apoA-I was negligible, and ABCA1 silencing with siRNA or inhibition with vanadate and Probucol did not inhibit the efflux to apoA-I, HDL, or plasma. Cholesterol efflux from and cholesterol uptake by RBCs from Abca1+/+ and Abca1−/− mice were similar, arguing against the role of ABCA1 in cholesterol flux between RBCs and lipoproteins. Bioinformatics analysis identified ABCA7, ABCG5, lipoprotein lipase, and mitochondrial translocator protein as possible candidates that may mediate the cholesterol flux. Together, these results suggest that RBCs actively participate in cholesterol transport in the blood, but the role of cholesterol transporters in RBCs remains uncertain.

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Levels, J. H. M., P. R. Abraham, A. van den Ende, and S. J. H. van Deventer. "Distribution and Kinetics of Lipoprotein-Bound Endotoxin." Infection and Immunity 69, no. 5 (May 1, 2001): 2821–28. http://dx.doi.org/10.1128/iai.69.5.2821-2828.2001.

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ABSTRACT Lipopolysaccharide (LPS), the major glycolipid component of gram-negative bacterial outer membranes, is a potent endotoxin responsible for pathophysiological symptoms characteristic of infection. The observation that the majority of LPS is found in association with plasma lipoproteins has prompted the suggestion that sequestering of LPS by lipid particles may form an integral part of a humoral detoxification mechanism. Previous studies on the biological properties of isolated lipoproteins used differential ultracentrifugation to separate the major subclasses. To preserve the integrity of the lipoproteins, we have analyzed the LPS distribution, specificity, binding capacity, and kinetics of binding to lipoproteins in human whole blood or plasma by using high-performance gel permeation chromatography and fluorescent LPS of three different chemotypes. The average distribution of O111:B4, J5, or Re595 LPS in whole blood from 10 human volunteers was 60% (±8%) high-density lipoprotein (HDL), 25% (±7%) low-density lipoprotein, and 12% (±5%) very low density lipoprotein. The saturation capacity of lipoproteins for all three LPS chemotypes was in excess of 200 μg/ml. Kinetic analysis however, revealed a strict chemotype dependence. The binding of Re595 or J5 LPS was essentially complete within 10 min, and subsequent redistribution among the lipoprotein subclasses occurred to attain similar distributions as O111:B4 LPS at 40 min. We conclude that under simulated physiological conditions, the binding of LPS to lipoproteins is highly specific, HDL has the highest binding capacity for LPS, the saturation capacity of lipoproteins for endotoxin far exceeds the LPS concentrations measured in clinical situations, and the kinetics of LPS association with lipoproteins display chemotype-dependent differences.

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5

Garmish, O. "The nature of metabolic disorders of blood lipoproteins as the basis for the pathogenesis of atherosclerosis in patients with inflammatory joint diseases." Bukovinian Medical Herald 24, no. 4 (96) (November 26, 2020): 12–18. http://dx.doi.org/10.24061/2413-0737.xxiv.4.96.2020.97.

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Objective of this study was to determine the characteristics of the metabolic disorders of lipids and lipoproteins (LP) in the blood in 112 patients with systemic rheumatic diseases.Material and methods. In all patients, the level of C-reactive protein (CRP), the content of malonic aldehyde (MA) in circulating monocytes, in blood plasma, and catalase activity were determined. The presence and severity of pro-atherogenic status were evaluated by the content of modified low-density lipoproteins (LDL) and very-low-density lipoproteins (VLDL) in the blood, which was determined by the bioassay method using peritoneal mouse macrophages. The immunogenicity of modified LР was determined by the content in the circulating immune complexes (CIC) of cholesterol (Сhol) and triglycerides (TG). The spectrum of lipids and LP in the blood was evaluated in detail with an additional determination of the plasma level of proteins apoB and apoA-1 were determined.Results. The obtained results show the existence in the examined patients of significant systemic inflammation in conjunction with the distinct proatherogenic metabolic state that was revealed by lipoprotein modification with the appearance in them of auto-antigenic properties. These changes appeared despite the absence of significant traditional atherogenic risk factors. The results of the paired correlative analysis showed the existence of strong dependence between indexes of systemic inflammation, proatherogenic and immunogenic lipoprotein modification. Conclusions. When determining proatherogenic disorders of lipid and blood lipoproteins metabolism in patients with systemic rheumatic diseases, it is necessary to focus not on traditional risk factors, which may remain within normal values, but on the content of apoA-1, apoB proteins, their ratio, and the determination of modified lipoproteins blood and the severity of the autoimmune reaction to them.

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Arfuso, Francesca, Francesco Fazio, Michele Panzera, Claudia Giannetto, Simona Di Pietro, Elisabetta Giudice, and Giuseppe Piccione. "Lipid and lipoprotein profile changes in newborn calves in response to the perinatal period." Acta Veterinaria 67, no. 1 (March 1, 2017): 25–32. http://dx.doi.org/10.1515/acve-2017-0003.

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AbstractThe aim of this study was to evaluate the dynamic changes of serum lipid and lipoprotein profiles in 6 newborn calves during the first five days of life. From each calve blood sampling was performed daily starting from day 1 (after colostrum intake) until day 5 of life. Blood samples collected from each animal were tested for serum total lipids, phospholipids, non-esterified fatty acids (NEFAs), triglycerides, very low density lipoproteins (VLDLs), total cholesterol (Total-Chol), high density lipoproteins (HDLs) and low density lipoproteins (LDLs). One-way repeated measures analysis of variance (ANOVA) was applied to determine the effect of days of life on the studied parameters in calves. A statistically significant effect of days of life was found on all serum lipid and lipoprotein indices measured in calves with the exception of NEFAs that showed unchanged values throughout the monitoring period. The changes observed in calves during the early postnatal period are most likely due to the transition in energy sources, from a maternal nutrient supply comprising mainly carbohydrates and amino acids to the colostrum and milk diet rich in fat.

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Reffuveille, Fany, Charlène Leneveu, Sylvie Chevalier, Yanick Auffray, and Alain Rincé. "Lipoproteins of Enterococcus faecalis: bioinformatic identification, expression analysis and relation to virulence." Microbiology 157, no. 11 (November 1, 2011): 3001–13. http://dx.doi.org/10.1099/mic.0.053314-0.

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Enterococcus faecalis is a ubiquitous bacterium that is capable of surviving in a broad range of natural environments, including the human host, as either a natural commensal or an opportunistic pathogen involved in severe hospital-acquired infections. How such opportunistic pathogens cause fatal infections is largely unknown but it is likely that they are equipped with sophisticated systems to perceive external signals and interact with eukaryotic cells. Accordingly, being partially exposed at the cell exterior, some surface-associated proteins are involved in several steps of the infection process. Among them are lipoproteins, representing about 25 % of the surface-associated proteins, which could play a major role in bacterial virulence processes. This review focuses on the identification of 90 lipoprotein-encoding genes in the genome of the E. faecalis V583 clinical strain and their putative roles, and provides a transcriptional comparison of microarray data performed in environmental conditions including blood and urine. Taken together, these data suggest a potential involvement of lipoproteins in E. faecalis virulence, making them serious candidates for vaccine production.

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Hata, M., T. Ito, and K. Ohwada. "Kinetic analysis of apolipoproteins in postprandial hypertriglyceridaemia rabbits." Laboratory Animals 43, no. 2 (April 2009): 174–81. http://dx.doi.org/10.1258/la.2008.007004.

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The postprandial hypertriglyceridaemia (PHT) rabbit, developed as a new animal model of metabolic syndrome, is characterized by PHT, central obesity and glucose intolerance. For detailed investigation of lipid metabolism characteristics in PHT rabbit, the plasma levels of apolipoproteins A-I, B, C-II, C-III and E were measured. Movements of apolipoproteins B100 and B48 were investigated using sodium dodecyl sulphate–polyacrylamide gel electrophoresis to determine whether postprandially increased triglyceride is exogenous or endogenous. The level of apolipoproteins A-I, B, C-II and E were increased in PHT rabbit after feeding. Apolipoproteins B100 and B48 were detected in the plasma fraction of d < 1.006 g/mL of the PHT rabbit. The postprandial increase in apolipoprotein B in the PHT rabbit reflects a numerical increase in lipoprotein particles in the blood; the increase in apolipoproteins C-II and E suggests some disturbance in lipoprotein catabolism. Apolipoprotein B48 was detected postprandially in PHT rabbits. These results suggest that delayed catabolism of exogenous lipids caused the retention of chylomicron remnants in the blood. Results also suggest that activities of the lipolytic enzyme lipoprotein lipase and hepatic triglyceride lipase were deficient and that the hepatic uptake of exogenous lipoproteins was delayed in the PHT rabbit. Especially, for examining remnant hyperlipoproteinaemia in humans, PHT rabbit is an excellent animal model for hypertriglyceridaemia research.

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Et al., Alkhafajy. "A Molecular and Biochemical Study for Cholesteryl Ester Transfer Protein (CETP) Taq1B in Iraqi Patients with Hyperlipidemia." Baghdad Science Journal 16, no. 3(Suppl.) (September 22, 2019): 0747. http://dx.doi.org/10.21123/bsj.2019.16.3(suppl.).0747.

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Cholesteryl ester transfer protein gene contains some single nucleotide polymorphisms, which have been associated with serum high-density lipoprotein concentration and other lipoproteins. This study is done for determining of cholesteryl ester transfer protein polymorphism and evaluate its effect on serum lipid profile concentrations in some hyperlipidemic patients compared with healthy subjects in Salah Al-din governorate-Iraq. Blood samples were taken from (90) patients suffering from hyperlipidemia, and (70) samples that were apparently healthy controls. Serum lipid concentrations were measured by enzymatic assays. The polymorphism was genotyped using polymerase chain reaction restriction fragment length polymorphism analysis. The results showed that there was a significant decrease (P<0.05) in the frequency B2 allele, and B1B2, B2B2 genotype, and a significant increase (P<0.05) in the frequency B1 allele, and B1B1 genotype between patients and controls groups. There was a non-significant decrease in the levels of high density lipoproteins, total cholesterol, low density lipoproteins, and very low density lipoproteins levels, and non-significant increase in levels of triglycerides in individuals with the B1B1 genotype than in the B1B2 and B2B2 genotype. However, high density lipoproteins showed a significant decrease (P<0.001) between individuals with the B1B1 genotype and B2B2 genotype. Also, there was a non-significant difference in the levels of high density lipoproteins, total cholesterol, low density lipoproteins, and very low density lipoproteins levels, in individuals with the B1B2 genotype when compared with that of the B2B2 genotype.

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10

Dattilo, A. M., and P. M. Kris-Etherton. "Effects of weight reduction on blood lipids and lipoproteins: a meta-analysis." American Journal of Clinical Nutrition 56, no. 2 (August 1, 1992): 320–28. http://dx.doi.org/10.1093/ajcn/56.2.320.

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Dissertations / Theses on the topic "Blood lipoproteins – Analysis"

1

Sadeghian, Karen Wiese. "Influence of diet and exercise intensity on serum lipids and lipoproteins in young female runners." 1985. http://hdl.handle.net/2097/27572.

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Books on the topic "Blood lipoproteins – Analysis"

1

Kurt, Widhalm, and Naito Herbert K, eds. Detection and treatment of lipid and lipoprotein disorders of childhood: Proceedings of the Third International Atherosclerosis Conference, held in Vienna, Austria, April 4-9, 1983. New York: Liss, 1985.

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Nader, Rifai, Warnick G. Russell, and Dominiczak Marek H, eds. Handbook of lipoprotein testing. Washington, DC: AACC Press, 1997.

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Nader, Rifai, and Warnick G. Russell, eds. Laboratory measurement of lipids, lipoproteins, and apolipoproteins. Washington, D.C: AACC Press, 1994.

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(Editor), Nader Rifai, G. Russell Warnick (Editor), and Marek H. Dominiczak (Editor), eds. Handbook of Lipoprotein Testing. 2nd ed. AACC Press, 2001.

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5

Detection and treatment of lipid and lipoprotein disorders of childhood: Proceedings of the Third International Atherosclerosis Conference, held in Vienna, Austria, April 4-9, 1983. Liss, 1985.

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6

Berry, Michael N., Anthony M. Edwards, and Gregory J. Barritt. Isolated Hepatocytes Preparation, Properties and Applications (Laboratory Techniques in Biochemistry and Molecular Biology). Elsevier Publishing Company, 1991.

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Rusciano, D., and M. M. Burger. Cancer Metastasis: Experimental Approaches (Laboratory Techniques in Biochemistry and Molecular Biology). Elsevier Science, 2000.

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Diplock, Anthony T., and Catherine Rice-Evans. Techniques in Free Radical Research (Laboratory Techniques in Biochemistry and Molecular Biology). Elsevier Science & Technology, 1991.

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Synthetic Peptides as Antigens (Laboratory Techniques in Biochemistry and Molecular Biology). Elsevier Science, 1999.

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M, Zborowski, and Chalmers J. J, eds. Magnetic cell separation. Amsterdam: Elsevier, 2008.

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Book chapters on the topic "Blood lipoproteins – Analysis"

1

Angelberger, P. "Lipoprotein Labeling and Analysis Techniques." In Radiolabeled Blood Elements, 221–30. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2462-5_32.

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Plekhova, N. G., V. A. Nevzorova, T. A. Brodskay, K. I. Shakhgeldyan, B. I. Geltser, L. G. Priseko, I. N. Chernenko, and K. L. Grunberg. "Association of Cardiovascular Events and Blood Pressure and Serum Lipoprotein Indicators Based on Functional Data Analysis as a Personalized Approach to the Diagnosis." In Software Engineering Perspectives in Intelligent Systems, 278–93. Cham: Springer International Publishing, 2020. http://dx.doi.org/10.1007/978-3-030-63319-6_24.

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Sadykova, Dinara, Liliia Galimova, Evgeniia Slastnikova, Zulfiia Khabibrakhmanova, and Natalya Guseva. "Arterial Stiffness Assessment in Children with Familial Hypercholesterolemia." In Management of Dyslipidemia. IntechOpen, 2021. http://dx.doi.org/10.5772/intechopen.96018.

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Familial hypercholesterolemia (FH) is the genetic disease which characterized by an increase of level total cholesterol and low density lipoproteins since childhood. The aim of the study was to assess arterial stiffness in children with heterozygous FH by measuring the pulse wave velocity (PWV) in the aorta. The study involved 118 children, 60 healthy children in the control group and 58 children with heterozygous FH in the main group. Both groups were divided into 3 age subgroups: 5–7 years old, 8–12 years old and 13–17 years old. The diagnosis of FH was made using British criteria by Simon Broome. The lipid profile was determined for all children, blood pressure was monitored daily with an estimate of the minimum, mean and maximum PWV (PWVmin, mean PWV, PWVmax) in aorta using oscillometric method. Correlation analysis in patients with FH revealed direct correlation between PWVmin, mean PWV and PWVmax with total cholesterol (r = 0.46, r = 0.46 and r = 0.464, respectively, p < 0.001). The study demonstrates an increase in the PWV in the aorta in children with FH compared with healthy peers from 8–12 years of age and a progression of arterial stiffness most significant in the group of 13–17 years.

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Alhassan, Sofiya, and Peter Grandjean. "Essential Laboratory Methods for Blood Lipid and Lipoprotein Analysis." In Lipid Metabolism and Health, 117–45. CRC Press, 2005. http://dx.doi.org/10.1201/9781420038422.ch7.

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"39. Identification of cytokine and lipoprotein markers for analyses in SHARE Wave 6 dried blood spots." In Health and socio-economic status over the life course, 375–84. De Gruyter Oldenbourg, 2019. http://dx.doi.org/10.1515/9783110617245-039.

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Conference papers on the topic "Blood lipoproteins – Analysis"

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Damirchi, Behzad, Amir Rouhollahi, Salman Sohrabi, and Seyyed Mahdi Nemati Mehr. "Modeling and Stability Analysis of Truncated High Density Lipoprotein (HDL) System Using Martini Coarse Grain Technique." In ASME 2013 International Mechanical Engineering Congress and Exposition. American Society of Mechanical Engineers, 2013. http://dx.doi.org/10.1115/imece2013-64808.

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Lipoproteins are biochemical compounds containing both proteins and lipids. These particles carry chemicals like cholesterol and triglycerides that are not soluble in aqueous solutions. This paper presents modeling of lipoprotein system using coarse grain molecular dynamics technique and stability analysis of this system in a water solution like blood. A high density lipoprotein (HDL) that consists of two annular monomers is modeled. Also there are lipid bilayers located in center of the rings, so the whole HDL and lipid bilayers are called lipoprotein system. First, all atom model is provided and then coarse-grain model is obtained using MARTINI technique. Modeling of the system in all atom and coarse-grain is performed by VMD and simulation is executed by NAMD. System is simulated for 400ns with time step of 20fs in NPT ensemble. System temperature assumed similar to normal human body temperature. Finally the structure shape and stability of system were considered and results were analyzed.

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Zinman, B., C. Mathieu, S. Kaspers, HJ Woerle, and D. Fitchett. "Empagliflozin reduces mortality in analyses adjusted for control of blood pressure, low density lipoprotein cholesterol and HbA1c over time." In Diabetes Kongress 2018 – 53. Jahrestagung der DDG. Georg Thieme Verlag KG, 2018. http://dx.doi.org/10.1055/s-0038-1641901.

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Roan, Esra, Alex Bada, and Randy Buddington. "Mechanical Characterization of Preterm Neonate Pig Liver as a Function of High-Density Lipoprotein (HDL)." In ASME 2010 International Mechanical Engineering Congress and Exposition. ASMEDC, 2010. http://dx.doi.org/10.1115/imece2010-39363.

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Elastography, a non-invasive imaging modality, utilizes mechanical properties of tissue as markers for disease diagnosis or staging. In the case of liver, there have been a number of studies focusing on the relationship between elastic mechanical properties and underlying disease, i.e. fibrosis and cirrhosis. In summary, these studies indicate the feasibility of elastographic tools in detecting liver diseases such as fibrosis and steatosis. There have not been any studies looking at the mechanical properties of the preterm neonate liver to date, which is important, because preterm neonates are at a greater risk for developing liver complications due to their aggressive dietary needs that are met with total parenteral nutrition (TPN). They use of elastography may be less from the use of elastographic tools since the concerns over noise levels in measurements resulting from abdominal wall thickness may be less influential. Therefore, it is necessary to establish basic preterm neonate liver mechanical properties. In this study, we measured the nonlinear (hyperelastic) mechanical properties of livers from preterm pigs that were fed common neaonatal diets, i.e. colostrum, total parenteral nutrition (TPN). 16 neonate pigs survived the feeding regime. Mechanical evaluation of 15 of these neonatal pigs was achieved with the use of uniaxial compression experiments at 0.01 s−1 strain rate. The livers averaging a weight of 34.7±7.0 (SD), were stored in phosphate buffered saline solution at 4°C until experimentation, which occurred within 30 minutes of the animal sacrifice. A minimum of three specimens from each liver was required for the computation of averaged mechanical properties. In addition to mechanical testing samples, blood serum was also obtained from these animals and common chemical parameters for liver health were measured (bilirubin, ALT, AST, HDL, LDL, etc.) Exponential form of the hyperelastic strain energy function, W = b1exp[b2(L2 + 2/L-3)], where bi are the material parameters and L is the stretch ratio, was utilized to describe the hyperelastic mechanical behavior of the preterm neonate pig livers. With the use of E = 6b1b2, a small-strain regime estimate of the elastic modulus of the neonate liver tissue was also computed. The mean b1 and b2 parameters are determined to be 97.00±44.15(SD) Pa and 1.90±0.28(SD) (n = 71). The mean elastic modulus exhibited an linear dependence on the HDL values obtained from chemical analysis of the blood serum. Moreover, although relatively weak, the ratio of the HDL over LDL also correlated with the elastic modulus. To our knowledge, this is the only study to date that has focused on the mechanical properties of preterm neonatal pigs and its correlation with liver lipid profile in neonates. Future work will focus on correlating this information with histology and then devising multi-scale material characterization approaches that link underlying neonatal liver structure to its overall mechanical properties.

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