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1

Prior, Kimberley F., Benita Middleton, Alíz T. Y. Owolabi, et al. "Synchrony between daily rhythms of malaria parasites and hosts is driven by an essential amino acid." Wellcome Open Research 6 (October 20, 2021): 186. http://dx.doi.org/10.12688/wellcomeopenres.16894.2.

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Background: Rapid asexual replication of blood stage malaria parasites is responsible for the severity of disease symptoms and fuels the production of transmission forms. Here, we demonstrate that a Plasmodium chabaudi’s schedule for asexual replication can be orchestrated by isoleucine, a metabolite provided to the parasite in a periodic manner due to the host’s rhythmic intake of food. Methods: We infect female C57BL/6 and Per1/2-null mice which have a disrupted canonical (transcription translation feedback loop, TTFL) clock with 1×105 red blood cells containing P. chabaudi (DK genotype). We
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2

Prior, Kimberley F., Benita Middleton, Alíz T. Y. Owolabi, et al. "Synchrony between daily rhythms of malaria parasites and hosts is driven by an essential amino acid." Wellcome Open Research 6 (July 22, 2021): 186. http://dx.doi.org/10.12688/wellcomeopenres.16894.1.

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Background: Rapid asexual replication of blood stage malaria parasites is responsible for the severity of disease symptoms and fuels the production of transmission forms. Here, we demonstrate that the Plasmodium chabaudi’s schedule for asexual replication can be orchestrated by isoleucine, a metabolite provided to the parasite in periodic manner due to the host’s rhythmic intake of food. Methods: We infect female C57BL/6 and Per1/2-null TTFL clock-disrupted mice with 1×105 red blood cells containing P. chabaudi (DK genotype). We perturb the timing of rhythms in asexual replication and host fee
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3

Peletiri, Iseimokumo Christopher. "Validation of automated malaria parasite diagnostic machines based on first principle: A pre-requisite for acceptable results and treatment monitoring in resource limited settings." Annals of Medical Laboratory Science 2, no. 1 (2022): 35–41. http://dx.doi.org/10.51374/annalsmls.2022.2.1.0056.

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Background: Following the very recent introduction of automated malaria parasite diagnostic machines; the need to validate these high technology machines based on the first principle protocol in malaria parasite density determination for acceptable results and treatment monitoring cannot be over-emphasized. The aim of this review is to update Medical Laboratory Scientists, Medical Laboratory Technicians, and researchers alike on the first principle in the diagnosis of malaria using Giemsa stained thick and thin blood films and to build their capacity on how to validate any automated malaria pa
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4

Dimkpa, Felix Barikuura, Pheobe Akikoma Singer, Clement Ugochukwu Nyenke, Atoukaritou Osuosa, and Chidi Nduka Amadi-Ikpa. "Dynamics of <i>Plasmodium falciparum</i> Parasitaemia and Uncomplicated Malaria among School-Age Children living in Omoku, Southern Nigeria." Journal of Health, Applied Sciences and Management 6, no. 3 (2023): 59–67. http://dx.doi.org/10.4314/johasam.v6i3.8.

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This cross-sectional study determined the mean parasitaemia and prevalence of Plasmodium falciparum malaria parasites among school-aged children in Omoku, Ogba Egbema Ndoni Local Government Area, Rivers State, Southern Nigeria. A total of 200 blood samples were randomly collected through convenience sampling method from the study subjects. Malaria diagnosis was carried out using standard parasitological techniques. The demographic data of the subjects showed that more females, 126(63%), were examined compared with males, 74(37%). Overall prevalence of Plasmodium falciparum recorded was 49%. Ge
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Severins, Maite, Don Klinkenberg, and Hans Heesterbeek. "How selection forces dictate the variant surface antigens used by malaria parasites." Journal of The Royal Society Interface 9, no. 67 (2011): 246–60. http://dx.doi.org/10.1098/rsif.2011.0239.

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Red blood cells infected by the malaria parasite Plasmodium falciparum express variant surface antigens (VSAs) that evade host immunity and allow the parasites to persist in the human population. There exist many different VSAs and the differential expression of these VSAs is associated with the virulence (damage to the host) of the parasites. The aim of this study is to unravel the differences in the effect key selection forces have on parasites expressing different VSAs such that we can better understand how VSAs enable the parasites to adapt to changes in their environment (like control mea
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Jeyabal, L. "Hemogram interpretation and diagnosis of blood parasites of Canines." INTERNATIONAL JOURNAL OF AGRICULTURAL SCIENCES 18, no. 2 (2022): 864–69. http://dx.doi.org/10.15740/has/ijas/18.2/864-869.

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The organisms that live in the blood of their animal hosts are called as blood parasites and these parasites can range from single-celled protozoa to more complex bacteria and rickettsiae. The transmission varies, depending on the parasite, but often they are transmitted through the bites of ticks or flies. Various blood parasites such as Ehrlichia canis, Babesia canis, Hepatozoon canis and Trypanosoma evansi can cause serious thread in canines. The routine diagnosis in detection of various developmental stages of intracellular parasites with Romnowsky’s staining method would be a tough task o
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MARZAL, ALFONSO, ALEJANDRO IBÁÑEZ, MANUEL GONZÁLEZ-BLÁZQUEZ, PILAR LÓPEZ, and JOSÉ MARTÍN. "Prevalence and genetic diversity of blood parasite mixed infections in Spanish terrapins, Mauremys leprosa." Parasitology 144, no. 11 (2017): 1449–57. http://dx.doi.org/10.1017/s0031182017000889.

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SUMMARYBlood parasites such as haemogregarines and haemosporidians have been identified in almost all groups of vertebrates and may cause serious damages to their hosts. However, very little is known about biodiversity of these parasites and their effects on some groups of reptiles such as terrapins. Moreover, the information on virulence from blood parasites mixed infection is largely unknown in reptiles. With this aim, we investigated for the first time the prevalence and genetic diversity of blood parasites from one genus of haemoparasitic aplicomplexan (Hepatozoon) in two populations of Sp
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8

Slavic, Ksenija, Michael J. Delves, Miguel Prudêncio, et al. "Use of a Selective Inhibitor To Define the Chemotherapeutic Potential of the Plasmodial Hexose Transporter in Different Stages of the Parasite's Life Cycle." Antimicrobial Agents and Chemotherapy 55, no. 6 (2011): 2824–30. http://dx.doi.org/10.1128/aac.01739-10.

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ABSTRACTDuring blood infection, malarial parasites used-glucose as their main energy source. ThePlasmodium falciparumhexose transporter (PfHT), which mediates the uptake ofd-glucose into parasites, is essential for survival of asexual blood-stage parasites. Recently, genetic studies in the rodent malaria model,Plasmodium berghei, found that the orthologous hexose transporter (PbHT) is expressed throughout the parasite's development within the mosquito vector, in addition to being essential during intraerythrocytic development. Here, using ad-glucose-derived specific inhibitor of plasmodial hex
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MARTÍN, JOSÉ, MARIO GARRIDO, JESÚS ORTEGA, ROBERTO GARCÍA-ROA, ALEJANDRO IBÁÑEZ, and ALFONSO MARZAL. "Absence of haemoparasite infection in the fossorial amphisbaenian Trogonophis wiegmanni." Parasitology 143, no. 11 (2016): 1433–36. http://dx.doi.org/10.1017/s0031182016000950.

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SUMMARYBlood parasites such as haemogregarines and haemosporidians have been identified in almost all groups of vertebrates. However, very little is known about biodiversity of these parasites and their effects on some major groups of reptiles such as amphisbaenians, a distinctive group with many morphological and ecological adaptations to fossorial life. Conditions of the fossorial environment might also affect host–parasite relationships. We investigated the presence and the potential prevalence of three genera of haemoparasitic aplicomplexan blood parasites (Hepatozoon, Plasmodium and Haemo
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Silva, MAML, A. Ronconi, N. Cordeiro, et al. "Blood parasites, total plasma protein and packed cell volume of small wild mammals trapped in three mountain ranges of the Atlantic Forest in Southeastern Brazil." Brazilian Journal of Biology 67, no. 3 (2007): 531–35. http://dx.doi.org/10.1590/s1519-69842007000300019.

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A study of blood parasites in small wild non-flying mammals was undertaken in three areas of the Atlantic Forest in Southeastern Brazil: Serra de Itatiaia, RJ, Serra da Bocaina, SP and Serra da Fartura, SP, from June 1999 to May 2001. A total of 450 animals (15 species) were captured in traps and it was observed in 15.5% of the blood smears the presence of Haemobartonella sp. and Babesia sp. in red blood cells. There was no statistically significant difference between parasited and non-parasited specimens regarding total plasma protein, packed cell volume and body weight, which strongly sugges
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11

Uc-Cetina, Víctor, Carlos Brito-Loeza, and Hugo Ruiz-Piña. "Chagas Parasite Detection in Blood Images Using AdaBoost." Computational and Mathematical Methods in Medicine 2015 (2015): 1–13. http://dx.doi.org/10.1155/2015/139681.

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The Chagas disease is a potentially life-threatening illness caused by the protozoan parasite,Trypanosoma cruzi.Visual detection of such parasite through microscopic inspection is a tedious and time-consuming task. In this paper, we provide an AdaBoost learning solution to the task of Chagas parasite detection in blood images. We give details of the algorithm and our experimental setup. With this method, we get 100% and 93.25% of sensitivity and specificity, respectively. A ROC comparison with the method most commonly used for the detection of malaria parasites based on support vector machines
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Enslow, Chelsea, Rachel Vallender, Emily Rondel, and Nicola Koper. "Host dispersal and landscape conversion are associated with the composition of haemosporidian parasites of the golden-winged warbler." Parasitology 147, no. 1 (2019): 96–107. http://dx.doi.org/10.1017/s0031182019001240.

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AbstractUnderstanding factors that influence the spatial and temporal distributions of blood parasites is important to help predict how host species and their parasites may respond to global change. Factors that may influence parasite distributions are land cover and host dispersal patterns, which may result in exposure of a host to novel parasites, or escape from parasites of their origin. We screened golden-winged warblers from across the United States and Canada for blood parasites, and investigated whether land-use patterns or host dispersal affected the prevalence and composition of haemo
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13

Tewari, Rita, Solabomi A. Ogun, Ruwani S. Gunaratne, Andrea Crisanti, and Anthony A. Holder. "Disruption of Plasmodium berghei merozoite surface protein 7 gene modulates parasite growth in vivo." Blood 105, no. 1 (2005): 394–96. http://dx.doi.org/10.1182/blood-2004-06-2106.

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Abstract Merozoite invasion of red blood cells is crucial to the development of the parasite that causes malaria. Merozoite surface proteins (MSPs) mediate the first interaction between parasite and erythrocyte. In Plasmodium falciparum, they include a complex of products from at least 3 genes (msp1, msp6, and msp7), one of which, msp7, is part of a gene family containing 3 and 6 adjacent members in Plasmodium yoelii and Plasmodium falciparum, respectively. We have identified and disrupted msp7 in the Plasmodium berghei gene family. The protein is expressed in schizonts and colocalizes with MS
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14

Ramey, A. M., J. A. Reed, J. A. Schmutz, et al. "Prevalence, transmission, and genetic diversity of blood parasites infecting tundra-nesting geese in Alaska." Canadian Journal of Zoology 92, no. 8 (2014): 699–706. http://dx.doi.org/10.1139/cjz-2014-0041.

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A total of 842 blood samples collected from five species of tundra-nesting geese in Alaska was screened for haemosporidian parasites using molecular techniques. Parasites of the genera Leucocytozoon Danilewsky, 1890, Haemoproteus Kruse, 1890, and Plasmodium Marchiafava and Celli, 1885 were detected in 169 (20%), 3 (&lt;1%), and 0 (0%) samples, respectively. Occupancy modeling was used to estimate prevalence of Leucocytozoon parasites and assess variation relative to species, age, sex, geographic area, year, and decade. Species, age, and decade were identified as important in explaining differe
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15

Gabriel, G. O., A. A. Odumboni, and G. B. Dandara. "THE INCIDENCE OF PARASITOLOGICAL RESEARCH ON THE RUMINANT LIVESTOCK." FUOYE JOURNAL OF AGRICULTURE AND HUMAN ECOLOGY 5, no. 2 (2023): 38–44. http://dx.doi.org/10.62923/fuojahe.v5i2.187.

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This study's goal is to identify external, blood, and Gastrointestinal (G.I) parasites in the farm animals at the Nasarawa State University Research farm, in Lafia, Nigeria. Blood sample was collected through a cephalic vein using the needle and syringe, External parasites were hand-picked directly from the animal body with the aid of hand gloves. Faecal Sample were collected from 270 Cattle, 500 sheep and 700 Goats via their rectum, using clean, unused disposable hand gloves. These methods were utilized to detect Helminthic eggs and Coccidia oocysts: Haematocrit Centrifugation (HCT) were used
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16

Cowman, Alan F., and Brendan S. Crabb. "Invasion of Red Blood Cells by Malaria Parasites." Cell 124, no. 4 (2006): 755–66. https://doi.org/10.5281/zenodo.13534742.

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(Uploaded by Plazi for the Bat Literature Project) The malaria parasite is the most important member of the Apicomplexa, a large and highly successful phylum of intracellular parasites. Invasion of host cells allows apicomplexan parasites access to a rich source of nutrients in a niche that is largely protected from host defenses. All Apicomplexa adopt a common mode of host-cell entry, but individual species incorporate unique features and utilize a specific set of ligand-receptor interactions. These adhesins ultimately connect to a parasite actin-based motor, which provides the power for inva
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Cowman, Alan F., and Brendan S. Crabb. "Invasion of Red Blood Cells by Malaria Parasites." Cell 124, no. 4 (2006): 755–66. https://doi.org/10.5281/zenodo.13534742.

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(Uploaded by Plazi for the Bat Literature Project) The malaria parasite is the most important member of the Apicomplexa, a large and highly successful phylum of intracellular parasites. Invasion of host cells allows apicomplexan parasites access to a rich source of nutrients in a niche that is largely protected from host defenses. All Apicomplexa adopt a common mode of host-cell entry, but individual species incorporate unique features and utilize a specific set of ligand-receptor interactions. These adhesins ultimately connect to a parasite actin-based motor, which provides the power for inva
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18

Crilly, Nathan P., and Monica R. Mugnier. "Thinking outside the blood: Perspectives on tissue-resident Trypanosoma brucei." PLOS Pathogens 17, no. 9 (2021): e1009866. http://dx.doi.org/10.1371/journal.ppat.1009866.

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Trypanosoma brucei is a protozoan parasite that causes human and animal African trypanosomiases (HAT and AAT). In the mammalian host, the parasite lives entirely extracellularly, in both the blood and interstitial spaces in tissues. Although most T. brucei research has focused on the biology of blood- and central nervous system (CNS)-resident parasites, a number of recent studies have highlighted parasite reservoirs in the dermis and adipose tissue, leading to a renewed interest in tissue-resident parasite populations. In light of this renewed interest, work describing tissue-resident parasite
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Margos, Gabriele, Sandra Navarette, Geoff Butcher, et al. "Interaction between Host Complement and Mosquito-Midgut-Stage Plasmodium berghei." Infection and Immunity 69, no. 8 (2001): 5064–71. http://dx.doi.org/10.1128/iai.69.8.5064-5071.2001.

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ABSTRACT After ingestion by mosquitoes, gametocytes of malaria parasites become activated and form extracellular gametes that are no longer protected by the red blood cell membrane against immune effectors of host blood. We have studied the action of complement onPlasmodium developmental stages in the mosquito blood meal using the rodent malaria parasite Plasmodium berghei and rat complement as a model. We have shown that in the mosquito midgut, rat complement components necessary to initiate the alternative pathway (factor B, factor D, and C3) as well as C5 are present for several hours follo
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Mirzaei, Farzaneh, Abolghasem Siyadatpanah, Roghayeh Norouzi, Soheila Pournasir, Veeranoot Nissapatorn, and Maria de Lourdes Pereira. "Blood Parasites in Domestic Birds in Central Iran." Veterinary Sciences 7, no. 3 (2020): 126. http://dx.doi.org/10.3390/vetsci7030126.

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Parasites may affect the dynamics of bird populations. Plasmodium, Leucocytozoon and Haemoproteus are well-known avian haematozoa that can trigger decreased productivity and high mortality in domesticated birds. In this study, we evaluated the prevalence of avian blood parasites (Plasmodium, Leucocytozoon and Haemoproteus) against 335 birds of 8 species in the Yazd province in central Iran. To detect blood parasites, Giemsa-stained blood smears were prepared. Of the birds, 11.64% (39/335) were infected with at least one parasite genus, particularly Haemoproteus (32.6%; 23/335). The total preva
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Lemy, E. E., A. G. Edafemakor, and A. O. Egwunyenga. "Socio-Demographic Characteristics and Blood Parasites Among Blood Donors in Two Communities in Delta State, Nigeria." Sokoto Journal of Medical Laboratory Science 7, no. 2 (2022): 85–94. http://dx.doi.org/10.4314/sokjmls.v7i2.10.

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A six months' study was conducted to determine the occurrence of blood parasites in blood donors in Eku and Abraka, Delta State. Random sampling was adopted for blood samples collection alongside socio-demographic characteristics. Laboratory analysis using thick and thin films were carried out on samples. The result showed that 59(29.5%) were positive for blood parasites; 46(23.0%) for Plasmodium, 5(5.0%) for Trypanosoma, and 8(4.0%) for microfilaria. Parasite prevalence was highest in the age-group 45-49 years. Female showed relatively higher prevalence (30.6%) compared to male (29.3%). There
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P, Mrs Gokila. "AI DRIVEN BLOOD CELL ANALYSIS FOR MALARIA AND BLOOD CANCER SCREENING." INTERANTIONAL JOURNAL OF SCIENTIFIC RESEARCH IN ENGINEERING AND MANAGEMENT 08, no. 05 (2024): 1–5. http://dx.doi.org/10.55041/ijsrem33052.

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Malaria and blood Cancer is one of the deadliest diseases cross the globe. This is caused by the bite of female Anopheles mosquito that transmits the Plasmodium parasites. Some current malaria detection techniques include manual microscopic examination and RDT. These approaches are vulnerable to human mistakes. Early detection of malaria can help in reducing the death rates across the globe.Deep Learning can emerge as a highly beneficial solution in the diagnosis of disease. This model gives a faster and cheaper method for detecting plasmodium parasites. It Was Designed to Identify Malaria Par
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Ritonga, Mudhita Z., Andhika Putra, Agun Prastia, Firdaus Nasution, and Risdawati Br Ginting. "Detection Of Blood Parasites In Cattle In Kutalimbaru Subdistrict, Deli Serdang Regency, North Sumatera." E3S Web of Conferences 151 (2020): 01040. http://dx.doi.org/10.1051/e3sconf/202015101040.

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The aim of this research was to identify and to find out the prevalence of blood parasites in cattle in Kutalimbaru Subdistrict, Deli Serdang Regency. This research was conducted in Kutalimbaru Subdistrict, Deli Serdang Regency from December 2018 to February 2019. Normal/healthy cattle of different breeds were randomly selected from five locations, namely Sei Mencirim village, Sawit Rejo village, Silebo-lebo village, Sampe Cita village and Pasar X village. A total of 150 blood samples were collected for blood smears. Diagnostic techniques were tested using Giemsa's staining technique. The bloo
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Rucksaken, Rucksak, Cherdsak Maneeruttanarungroj, Thanaporn Maswanna, Metita Sussadee, and Pithai Kanbutra. "Comparison of conventional polymerase chain reaction and routine blood smear for the detection of Babesia canis, Hepatozoon canis, Ehrlichia canis, and Anaplasma platys in Buriram Province, Thailand." Veterinary World 12, no. 5 (2019): 700–705. http://dx.doi.org/10.14202/vetworld.2019.700-705.

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Background and Aim: Dog blood parasites are important tick-borne diseases causing morbidity and mortality in dogs worldwide. Four dog blood parasites species are commonly found in Thailand: Babesia canis, Hepatozoon canis, Ehrlichia canis, and Anaplasma platys. They are transmitted easily by tick species. However, there is little prevalence data available in Thailand. Diseases presentation of blood parasites infection is similar, but the treatment of each species is different. Current diagnosis mainly relies on microscopic examination of a stained blood smear, which has low sensitivity. Theref
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T. Daoud, Yahya, Khalid M. Dakhil, and Hanaa Daaj Khalaf Al-Mozan. "Study the infection with intestinal parasites (Entamoeba histolytica) and its effect on the blood nature in children from Thi-Qar province." AL-QADISIYAH MEDICAL JOURNAL 10, no. 18 (2017): 152–58. http://dx.doi.org/10.28922/qmj.2014.10.18.152-158.

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The present study has included collection of 1001 fecal samples of children aged seven years and less than that age . 333 blood samples have been collected for the children that proved their infection by intestinal parasites from samples of study and 100 blood sample from non - infected who visited different hospitals in Thi – Qar Provence during the period from the first of October 2010 to the end of July 2011 to study the rate of intestinal parasites infection and their impact on certain criteria bloody . The fecal samples have been examined by two methods are: direct smear using Normal Sa
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Harl, Josef, Anaïs Fauchois, Marie-Pierre Puech, et al. "Novel phylogenetic clade of avian Haemoproteus parasites (Haemosporida, Haemoproteidae) from Accipitridae raptors, with description of a new Haemoproteus species." Parasite 31 (2024): 5. http://dx.doi.org/10.1051/parasite/2023066.

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Avian haemosporidian parasites (order Haemosporida, phylum Apicomplexa) are blood and tissue parasites transmitted by blood-sucking dipteran insects. Three genera (Plasmodium, Haemoproteus and Leucocytozoon) have been most often found in birds, with over 270 species described and named in avian hosts based mainly on the morphological characters of blood stages. A broad diversity of Haemoproteus parasites remains to be identified and characterized morphologically and molecularly, especially those infecting birds of prey, an underrepresented bird group in haemosporidian parasite studies. The aim
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Quillfeldt, Petra, Tanja Romeike, Juan F. Masello, Gerald Reiner, Hermann Willems, and Yuliana Bedolla-Guzmán. "Molecular survey of coccidian infections of the side-blotched lizard Uta stansburiana on San Benito Oeste Island, Mexico." Parasite 25 (2018): 43. http://dx.doi.org/10.1051/parasite/2018043.

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Blood parasites are found in many vertebrates, but the research on blood parasites of lizards is still at its onset. We analyzed blood samples from side-blotched lizards Uta stansburiana from San Benito Oeste Island, Mexico, to test for the presence of hemoparasites. We found a high prevalence (23 out of 27 samples) of a blood parasite of the genus Lankesterella (Coccidia, Eimeriorina, Lankesterellidae) according to phylogenetic analyses of the parasite 18S rRNA gene. Similar parasites (97–99% similarity) have recently been described for Uta stansburiana from California. The parasite 18S rRNA
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Legowo, Djoko, Syifa Husnul Khotimah, and Lucia Tri Suwanti. "Blood Parasite Infection Prevalence in Kampong Chicken Breeder’s Group in Garut." KnE Life Sciences 3, no. 6 (2017): 308. http://dx.doi.org/10.18502/kls.v3i6.1139.

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The Aim of this research was to determine the blood parasite infection prevalence in Garut District. The research was conducted in November-December 2016. 160 samples of blood were obtained from eight subdistrict at Kampong Chicken Breeder’s Group (KEPAK) in Garut District smeared with 3% Giemsa solution and examined under a microscope with 1000x magnification. The merozoite and gametocyte that was discovered recorded. The data that obtained was analyzed descriptively. A parasites were found in 11 blood samples (6.88%). The genus of blood parasites is Leucocytozoon sp., while Plasmodium sp., H
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Ozwara, Hastings, Jan A. M. Langermans, Clemens H. M. Kocken, et al. "Transfected Plasmodium knowlesi Produces Bioactive Host Gamma Interferon: a New Perspective for Modulating Immune Responses to Malaria Parasites." Infection and Immunity 71, no. 8 (2003): 4375–81. http://dx.doi.org/10.1128/iai.71.8.4375-4381.2003.

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ABSTRACT Transgenic pathogenic microorganisms expressing host cytokines such as gamma interferon (IFN-γ) have been shown to manipulate host-pathogen interaction, leading to immunomodulation and enhanced protection. Expression of host cytokines in malaria parasites offers the opportunity to investigate the potential of an immunomodulatory approach by generating immunopotentiated parasites. Using the primate malaria parasite Plasmodium knowlesi, we explored the conditions for expressing host cytokines in malaria parasites. P. knowlesi parasites transfected with DNA constructs for expressing rhes
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Tetteh, Ato Kwamena, Sadick Arthur, Prince Bram, Charles Baffe, and Godsway Aglagoh. "Prevalence of Asymptomatic Malaria Parasitemia among Blood Donors in Cape Coast, Ghana: A Cross-Sectional Study." Journal of Tropical Medicine 2023 (January 4, 2023): 1–6. http://dx.doi.org/10.1155/2023/8685482.

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Background. Malaria is an important transfusion-associated infection in many parts of the world, particularly in sub-Saharan Africa, where it is endemic. We studied the prevalence of malaria parasites among blood donors in the Cape Coast Metropolitan Area. Methods. A malaria parasite examination was added to the blood donor screening protocol for 240 voluntary and replacement blood donors (224 males and 16 females) between December 2020 and July 2021. Results. Overall, 2.5% (6/240) had Plasmodium falciparum trophozoites detected in their blood sample. The remaining had no parasites detected. F
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Miller, Jessica L., Anke Harupa, Stefan H. I. Kappe, and Sebastian A. Mikolajczak. "Plasmodium yoelii Macrophage Migration Inhibitory Factor Is Necessary for Efficient Liver-Stage Development." Infection and Immunity 80, no. 4 (2012): 1399–407. http://dx.doi.org/10.1128/iai.05861-11.

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ABSTRACTMammalian macrophage migration inhibitory factor (MIF) is a multifaceted cytokine involved in both extracellular and intracellular functions. Malaria parasites express a MIF homologue that might modulate host immune responses against blood-stage parasites, but the potential importance of MIF against other life cycle stages remains unstudied. In this study, we characterized the MIF homologue ofPlasmodium yoeliithroughout the life cycle, with emphasis on preerythrocytic stages.P. yoeliiMIF (Py-MIF) was expressed in blood-stage parasites and detected at low levels in mosquito salivary gla
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Blythe, Jane E., Xue Yan Yam, Claudia Kuss, et al. "Plasmodium falciparum STEVOR Proteins Are Highly Expressed in Patient Isolates and Located in the Surface Membranes of Infected Red Blood Cells and the Apical Tips of Merozoites." Infection and Immunity 76, no. 7 (2008): 3329–36. http://dx.doi.org/10.1128/iai.01460-07.

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ABSTRACT The human parasite Plasmodium falciparum has the potential to express a vast repertoire of variant proteins on the surface of the infected red blood cell (iRBC). Variation in the expression pattern of these proteins is linked to antigenic variation and thereby evasion of host antibody-mediated immunity. The genes in the stevor multigene family code for small variant antigens that are expressed in blood-stage parasites where they can be detected in membranous structures called Maurer's clefts (MC). Some studies have indicated that STEVOR protein may also be trafficked to the iRBC membr
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Cruz, Kathlyn, Joasanne Mae Maguigad, Mary Ann Santos, Jomar Allam, Joel Casibang, and Jomel Manuel. "SURVEILLANCE OF BLOOD PARASITES OF NATURALLY-GROWN SMALL RUMINANTS IN SELECTED PROVINCE OF REGION 02." International Journal of Research and Technology in Agriculture and Fisheries 1, no. 1 (2021): 13–21. http://dx.doi.org/10.55687/ijrtaf.v1i1.3.

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This study aimed to determine the epidemiology and surveillance of blood parasites of naturally-grown small ruminants in selected province of Region 02. Descriptive method through a survey questionnaire and a blood parasite examination was employed. Survey showed that, there were 23 naturally-grown small ruminant farms located at Districts II and III in Cagayan province. Anaplasma spp. and Babesia spp. were the species of blood parasite identified. Test of difference showed that presence of blood parasites was significant with sex of the naturally-grown small ruminants and between Districts. F
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Schetters. "Mechanisms Involved in the Persistence of Babesia canis Infection in Dogs." Pathogens 8, no. 3 (2019): 94. http://dx.doi.org/10.3390/pathogens8030094.

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Dogs that are infected with Babesia canis parasites usually show severe clinical signs, yet often very few parasites are detectable in the blood circulation. The results showed that large numbers of B. canis-infected red blood cells accumulate in the microvasculature of infected subjects. The initial process leading to the attachment of infected erythrocytes to the endothelial cells of small capillaries (sequestration) appears to involve the interaction of parasite molecules at the erythrocyte surface with ligands on the endothelial cells. Since parasites continue to develop in the sequestered
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Bennett, Gordon F., Vernon D. Stotts, and Myrtle C. Bateman. "Blood parasites of black ducks and other anatids from Labrador and insular Newfoundland." Canadian Journal of Zoology 69, no. 5 (1991): 1405–7. http://dx.doi.org/10.1139/z91-198.

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A total of 510 ducks of seven species from Labrador was examined for blood parasites; 76% of the birds were parasitized by one or more haematozoa. Leucocytozoon simondi occurred in 91% whereas haemoproteids occurred in only 11% of the infected ducks. Green-winged teal and black ducks were the most heavily parasitized of the anatid species, with a prevalence of infection higher than that of either pintails or mallards. Only 49% of the same species of ducks from western Newfoundland were infected with blood parasites; haemoproteids, rather than leucocytozoids, were the most commonly encountered
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36

Magaña Vázquez, Regina, Friederike Woog, Anke Dinkel, Ute Mackenstedt, and Sandrine Musa. "Blood Parasites of Vangas and Other Corvoidea on Madagascar." Diversity 14, no. 10 (2022): 888. http://dx.doi.org/10.3390/d14100888.

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Madagascar hosts a great diversity of bird species. This study focuses on the description of the diversity and prevalence of blood parasites (Haemosporida, trypanosomes and filarioid nematodes) in 131 blood samples of 14 species of Corvoidea, namely vangas (Vangidae), Coracina cinerea (Campephagidae), Dicrurus forficatus (Dicruridae) and Terpsiphone mutata (Monarchidae) found in primary rainforests on Madagascar. Blood parasites were detected using both molecular and microscopic methods. Multiplex PCR was used to detect mixed haemosporidian infections and nested PCR was used to describe a 479
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Ngwu, Amauche Martina. "Microscopic Diagnosis of Malaria Parasitaemia in Pregnant Women." European Journal of Theoretical and Applied Sciences 1, no. 2 (2023): 269–74. http://dx.doi.org/10.59324/ejtas.2023.1(2).23.

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Malaria is the major cause of morbidity and mortality in the developing countries in which Nigeria is one of them. Malaria during pregnancy is a major cause of maternal death globally. Microscopic analysis of Giemsa stained blood film is still the most suitable method of malaria diagnosis in most developing countries. The aim of this study was to determine malaria parasites number per 100 thick film fields in a Giemsa stained blood film. This is a cross sectional study involving 90 pregnant women within the ages of 19-45 years. Thick and thin smears were prepared on clean glass slide using ven
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Amauche, Martina Ngwu. "Microscopic Diagnosis of Malaria Parasitaemia in Pregnant Women." European Journal of Theoretical and Applied Sciences 1, no. 2 (2023): 269–74. https://doi.org/10.59324/ejtas.2023.1(2).23.

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Malaria is the major cause of morbidity and mortality in the developing countries in which Nigeria is one of them. Malaria during pregnancy is a major cause of maternal death globally. Microscopic analysis of Giemsa stained blood film is still the most suitable method of malaria diagnosis in most developing countries. The aim of this study was to determine malaria parasites number per 100 thick film fields in a Giemsa stained blood film. This is a cross sectional study involving 90 pregnant women within the ages of 19-45 years. Thick and thin smears were prepared on clean glass slide using ven
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Jarra, William, and Georges Snounou. "Only Viable Parasites Are Detected by PCR following Clearance of Rodent Malarial Infections by Drug Treatment or Immune Responses." Infection and Immunity 66, no. 8 (1998): 3783–87. http://dx.doi.org/10.1128/iai.66.8.3783-3787.1998.

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ABSTRACT Detection and analysis of pathogens by PCR plays an important role in infectious disease research. The value of these studies would be diminished if nuclear material from dead parasites were found to remain in circulation for extended periods and thus result in positive amplification. This possibility was tested in experimental rodent malaria infections. Blood samples were obtained from infected mice during and following drug or immune clearance of Plasmodium chabaudi chabaudi parasitemias. Detection of parasite DNA by a sensitive Plasmodium-specific PCR amplification assay was associ
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Chagas, Carolina Romeiro Fernandes, Mélanie Duc, Germán Alfredo Gutiérrez-Liberato, and Gediminas Valkiūnas. "Host Cells of Leucocytozoon (Haemosporida, Leucocytozoidae) Gametocytes, with Remarks on the Phylogenetic Importance of This Character." Pathogens 12, no. 5 (2023): 712. http://dx.doi.org/10.3390/pathogens12050712.

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Leucocytozoon parasites remain poorly investigated in comparison to other haemosporidians. The host cell inhabited by their blood stages (gametocytes) remains insufficiently known. This study aimed to determine the blood cells inhabited by Leucocytozoon gametocytes in different species of Passeriformes and to test if this feature has a phylogenetic importance. We microscopically analyzed blood films stained with Giemsa from six different bird species and individuals and used PCR-based methods for parasite lineage identification. The DNA sequences obtained were applied for phylogenetic analysis
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Sebaio, Fabiane, Érika Martins Braga, Felipe Branquinho, Alan Fecchio, and Miguel Ângelo Marini. "Blood parasites in passerine birds from the Brazilian Atlantic Forest." Revista Brasileira de Parasitologia Veterinária 21, no. 1 (2012): 7–15. http://dx.doi.org/10.1590/s1984-29612012000100003.

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Parasites may lead bird species to extinction, affect host temporal and spatial population dynamics, alter community structure and alter individuals’ social status. We evaluated blood parasite prevalence and intensity according to bird families and species, among 925 birds that were caught in 2000 and 2001, in the Atlantic Forest in the State of Minas Gerais, Brazil. We applied Giemsa staining to thin blood smears, to detect blood parasites. The birds (n = 15.8%) in 11 families, were infected by at least one parasite genus, especially Muscicapidae (28.3%) and Conopophagidae (25%). Among the 14
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Srisai, Prakit, Manvika Pholpark, Pichet Tongpan, et al. "Prevalence of Blood Parasites Infestation among Buffaloes with Abortion in Nakhon Phanom Province, Thailand." Outbreak, Surveillance, Investigation & Response (OSIR) Journal 10, no. 1 (2017): 7–15. http://dx.doi.org/10.59096/osir.v10i1.263189.

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From 2007 to 2011, the buffalo population in Nakhon Phanom Province decreased 34% with approximately 150-300 aborted buffaloes each year. However, the etiology was not systematically examined. Our objectives were to determine prevalence of blood parasites in buffaloes with abortion, and identify causes and possible factors related to abortion. We conducted a case control study in Nakhon Phanom from April 2011 to March 2012. Cases were buffaloes that reported abortion and controls were female buffaloes that previously calved without history of abortion in the same sub-district with the case. Fa
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SOARES, LETÍCIA, VINCENZO A. ELLIS, and ROBERT E. RICKLEFS. "Co-infections of haemosporidian and trypanosome parasites in a North American songbird." Parasitology 143, no. 14 (2016): 1930–38. http://dx.doi.org/10.1017/s0031182016001384.

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SUMMARYHosts frequently harbour multiple parasite infections, yet patterns of parasite co-occurrence are poorly documented in nature. In this study, we asked whether two common avian blood parasites, one haemosporidian and one trypanosome, affect each other's occurrence in individuals of a single host species. We used molecular genotyping to survey protozoan parasites in the peripheral blood of yellow-breasted chats (Aves: Passeriformes [Parulidae]:Icteria virens) from the Ozarks of Southern Missouri. We also determined whether single and co-infections differently influence white blood cell an
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44

Silva-Iturriza, Adriana. "History of the Study of Avian Haemosporidian Parasites in A Neotropical Country: Venezuela." International Journal of Zoology and Animal Biology 6, no. 2 (2023): 1–6. http://dx.doi.org/10.23880/izab-16000452.

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Venezuela has a remarkable presence of endemic birds from the Neotropical region. Therefore, the study of avian haemosporidian parasites (Plasmodium, Haemoproteus, Leucocytozoon) in Venezuela should be a priority task for conservation projects, as well as for understanding adaptive processes of the parasite-host-vector triad. The objective of this mini-review is to rescue the most relevant contributions of the study of these parasites in Venezuela and the potentialities derived from them. The greatest contribution reported so far comes from a series of parasitological diagnoses in birds carrie
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Journal, Baghdad Science. "Clinico – Hemato and Biochemical Study of Anemia in Cachectic Cattle in Baghdad." Baghdad Science Journal 8, no. 4 (2011): 877–86. http://dx.doi.org/10.21123/bsj.8.4.877-886.

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The study was carried out in order to evaluate clinically and laboratory cachectic animals suffering from anemia. Animal examined were 50 cow and calf. The study include clinical, hemato and biochemical test for accurate diagnosis of cachexia in cows and calves . Blood smears were conducted for detection of blood parasites , fecal examination for gastrointestinal parasites and Different parameters were applied for classification of cachexia , depending on bony projection specially ribs and pelvic and generalized muscular atrophy. However , The study revealed an incidence of cachexia and anemia
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46

Carbayo, J., J. Martín, and E. Civantos. "Habitat type influences parasite load in Algerian Psammodromus (Psammodromus algirus) lizards." Canadian Journal of Zoology 97, no. 2 (2019): 172–80. http://dx.doi.org/10.1139/cjz-2018-0145.

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Understanding how habitat type and deterioration may affect parasitism is important in assessing the effects of human-induced environmental change on host–parasite dynamics. In this study, we examined inter-population differences in parasite load in the Mediterranean lizard Psammodromus algirus (Linnaeus, 1758). We analyzed prevalence and intensity of infection by blood parasites and ectoparasites in two populations separated by a 400 m elevational gradient and with different habitat types that also differed in the degree of human alteration. We also compared data obtained from the same popula
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Ellis, Vincenzo A., Michael D. Collins, Matthew C. I. Medeiros, et al. "Local host specialization, host-switching, and dispersal shape the regional distributions of avian haemosporidian parasites." Proceedings of the National Academy of Sciences 112, no. 36 (2015): 11294–99. http://dx.doi.org/10.1073/pnas.1515309112.

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The drivers of regional parasite distributions are poorly understood, especially in comparison with those of free-living species. For vector-transmitted parasites, in particular, distributions might be influenced by host-switching and by parasite dispersal with primary hosts and vectors. We surveyed haemosporidian blood parasites (Plasmodium and Haemoproteus) of small land birds in eastern North America to characterize a regional parasite community. Distributions of parasite populations generally reflected distributions of their hosts across the region. However, when the interdependence betwee
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Vincendeau, P., and M. Daëron. "Trypanosoma musculi co-express several receptors binding rodent IgM, IgE, and IgG subclasses." Journal of Immunology 142, no. 5 (1989): 1702–9. http://dx.doi.org/10.4049/jimmunol.142.5.1702.

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Abstract The present work demonstrates the expression of receptors for the Fc portion of rodent Ig by the murine parasite Trypanosoma musculi. By using a rosette assay adapted to the parasite morphology and by flow cytometry analysis, three distinct receptors were identified. A receptor binding rabbit or rat polyclonal IgG and mouse monoclonal IgG1, IgG2a, and IgG2b was found on parasites purified from the blood and the peritoneal cavity of infected mice and on parasites maintained in culture conditions. This IgG receptor was degraded by pepsin. A separate receptor, binding only mouse monoclon
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Webb, S. E., R. E. Fowler, C. O'Shaughnessy, et al. "Contractile protein system in the asexual stages of the malaria parasite Plasmodium falciparum." Parasitology 112, no. 5 (1996): 451–57. http://dx.doi.org/10.1017/s0031182000076915.

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SUMMARYF-actin was detected in asexual-stage Plasmodium falciparum parasites by fluorescence microscopy of blood films stained with fluorescent phalloidin derivatives. F-actin was present at all stages of development and appeared diffusely distributed in trophic parasites, but merozoites stained strongly at the poles and peripheries. No filament bundles could be discerned. A similar distribution was obtained by immunofluorescence with 2 polyclonal anti-actin antibodies, one of which was directed against a peptide sequence present only in parasite actin (as inferred from the DNA sequence of the
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Rajasekariah, G.-Halli, Samuel K. Martin, Anthony M. Smithyman, and Bernard J. Hudson. "Relevance of Plasmodium falciparum Biomarkers in the Treatment and Control of Malaria." European Journal of Medical and Health Sciences 5, no. 1 (2023): 31–40. http://dx.doi.org/10.24018/ejmed.2023.5.1.1450.

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We report here a dual-ELISA method to measure Malaria biomarkers concurrently in the same aliquot of blood sample. A correlation between the parasite numbers and ELISA values determined and the figures were used to establish a standard graph. Thick blood smears prepared from spiked blood samples were also Giemsa stained and parasite density determined by microscopy (It was thereby possible to undertake an objective comparison between lactate dehydrogenase and histidine- rich- proteins levels assessed by ELISA and parasite density determined by microscopy from the same spiked aliquot). The pres
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