Academic literature on the topic 'Blood sugar ; Hypothalamus ; Hydrogen peroxide'

The potential acute genoprotective effect of orange juice supplementation was investigated. Six healthy subjects (aged 33 to 60 years; 3 women and 3 men) were asked to drink 400 mL of commercial orange juice, which contained 100 mg vitamin C and 40.8 g sugar. Venous blood (2 mL) was taken before and 2 h after ingestion (test trial). A week later, the subjects were asked to repeat the trial by drinking 400 mL water with 100 mg vitamin C and 40.8 g glucose (control trial). Lymphocytes isolated from blood samples underwent comet assay on the day of collection. Pre- and postingestion DNA damage scores were measured in both the test and control trials. Results showed that there was a significant decrease in DNA damage induced by hydrogen peroxide after 2 h of supplementation with orange juice, and no change in baseline DNA damage. There was no significant decrease in the DNA damage in lymphocytes in the control trial.

Nanowires of carbon coated ferric oxide (Fe2O3/C) have been studied to be applied at an electrochemical glucose biosensor as a new type of carbon paste electrode (CPE) modifier. The Fe2O3/C was synthesized by adapting co-precipitation hydrothermal route. Firstly, amperometric sensor based on a modified CPE doped with Fe2O3 has been developed and compared with CPE doped with Fe2O3/C for hydrogen peroxide (H2O2) determination. The performance evaluations for the modified materials and sensors were studied in detail through cyclic voltammetry (CV) method. After, the electrochemical sensor performance was also evaluated for glucose oxidase (GOx) response biosensor due to considerable interest in the blood sugar control. The results demonstrate that the GOx retains its biocatalytic activity toward the oxidation of glucose and that the bioelectrode modified by the Fe2O3/C matrix has potential for use in biosensors and other bioelectronics devices.

Honey is one of the traditional medicine that can be used in the community for wound treatment since centuries ago. Honey has an antibacterial effect, due to its high osmolarity , hydrogen peroxide content, low pH and low activity. The pH of honey is acidic (ranges about 3.2-4.5), inhibits the metabolism of Gram negative and positive bacteria, in consequence would be bacterial metabolism inhibition. This condition would lead to bacterial lysis. Honey can be used as wound treatment, controls blood sugar in diabetes patient`s, antioxidants, prevents inflammation outer layer surrounding the mouth of the laser beam treatment of cancer patients, treats gastrointestinal inflammation, as well as the material beauty products, hair and skin. This study was conducted to determine the comparative effectiveness of honey solution on the growth of Staphylococcus aureus and Pseudomonas aeruginosae with various concentrations of 105 ppm, 106 ppm , 5x106 ppm , 107 ppm , and 108 ppm by well diffusion method. According to the MIC result on S .aureus, its is included in weak category, with an average diameter 6.5 mm , 10.5 mm, 12 mm, and 14.7 mm on 106 ppm, 5x106 ppm, 107 ppm, and 108 ppm, respectively. Based on the MIC result on Pseudomonas aeruginosae, it also included in weak category, with an average diameter 6 mm , 8.5 um , 9.5 mm, 10.5 mm on 106 ppm, 5x106 ppm on 106 ppm, 5x106 ppm, 107 ppm, and 108 ppm, respectively. This study concluded that antibacterial from honey solution is more effective against Staphylococcus aureus than Pseudomonas aeruginosae, although there are no significantly difference.

This is summarized in Table 8.1. a) Microscopy— A cell count is performed on sterile fluids and CSF samples using the Neubauer chamber or a similar device. The number of white blood cells (WBC) and red blood cells seen under the microscope are reported as well as the differential WBC count (i.e. the number or percentage of lymphocytes and neutrophils in the sample). A Gram stain is then done and the presence of any organism reported. b) Culture samples are plated onto the appropriate media and streaked out for single colonies as shown. Blood agar is normally used; however, other media are used depending on the site of the specimen, e.g. chocolate agar is used if a fastidious organism is a potential pathogen such as Haemophilus sp.; anaerobic agar for anaerobes; selective agar such as MacConkey can be used on non-sterile specimens to differentiate between the colony types. Plates are incubated for eighteen to forty-eight hours at the correct conditions; most plates being CO2, others at O2 and anaerobically. c) Identification plates are examined for growth. Potentially significant isolates are identified either by MALDI-TOF MS, by API, or other biochemical tests. d) Sensitivities are performed on significant organisms by manual and automated methods. This is summarized in Table 8.2. Selective agar is necessary when isolating pathogens from faeces, although further confirmatory tests are needed. ● Black or colourless colonies on xylose lysine deoxycholate (XLD) or other chromogenic agar plates are tested with oxidase reagent. ● Oxidase negative isolates are identified by MALDI-TOF, API and or biochemically using triple-sugar iron (TSI) tubes. ● Serology is then performed on suspicious isolates and sent to a reference laboratory for confirmation. ● Campylobacter is confirmed by testing grey flat colonies on campylobacter agar with oxidase reagent. Oxidase positive samples are Gram stained and if ‘seagull’-shaped gram-negative bacteria are observed under the microscope, campylobacter is confirmed. The catalase test is a simple biochemical test to differentiate between Staphylococcus species and Streptococcus species, with the use of hydrogen peroxide (H2O2). It tests for the presence of the enzyme catalase which is found in Staphylococcus species.