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Journal articles on the topic 'Blunt end'

Author: Grafiati

Published: 5 June 2025

Last updated: 2 August 2025

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1

Riddihough, G. "Blunt End Protection." Science 337, no. 6096 (2012): 778. http://dx.doi.org/10.1126/science.337.6096.778-d.

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2

Sambrook, Joseph, and David W. Russell. "Blunt-end Cloning of PCR Products." Cold Spring Harbor Protocols 2006, no. 1 (2006): pdb.prot3830. http://dx.doi.org/10.1101/pdb.prot3830.

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3

Dongming, Lan, Yan Huiling, Yang Bo, and Wang Yonghua. "Blunt-end vectors generated by polymerase chain reaction (PCR) for direct cloning of blunt-end DNA fragments." African Journal of Biotechnology 10, no. 54 (2011): 11149–51. http://dx.doi.org/10.5897/ajb10.1369.

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4

Kazda, A., B. Zellinger, M. Rossler, E. Derboven, B. Kusenda, and K. Riha. "Chromosome end protection by blunt-ended telomeres." Genes & Development 26, no. 15 (2012): 1703–13. http://dx.doi.org/10.1101/gad.194944.112.

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5

Green, Michael R., and Joseph Sambrook. "Cloning in Plasmid Vectors: Blunt-End Cloning." Cold Spring Harbor Protocols 2020, no. 11 (2020): pdb.prot101246. http://dx.doi.org/10.1101/pdb.prot101246.

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6

Nybo, Kristie. "DNA and General PCR Methods: Blunt-end Ligation." BioTechniques 48, no. 4 (2010): 273–75. http://dx.doi.org/10.2144/000113397.

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7

Zhang, Rong, Shu Cai Wang, Kai Zhang, and Xiong Feng Xie. "Compressive Properties Study and Finite Element Analysis on Duck Eggshell." Applied Mechanics and Materials 618 (August 2014): 598–602. http://dx.doi.org/10.4028/www.scientific.net/amm.618.598.

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The mechanical properties of the sharp end, equator and blunt end of White-duck-eggshell were tested through the compression experiment, and the static load mechanical properties of the eggshell were simulated with finite element analysis method. The results showed that the maximum pressure that the loaded long axis of the eggshell can endure is obviously greater than that of the short axis, and that the maximum pressure that the sharp end can endure is greater than that of the blunt end in the long axis. Furthermore, the same results have been obtained both by finite element analysis (FEA) an

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8

D'Souza, Cynthia R., Ken V. Deugau, and John H. Spencer. "A simplified procedure for cDNA and genomic library construction using nonpalindromic oligonucleotide adaptors." Biochemistry and Cell Biology 67, no. 4-5 (1989): 205–9. http://dx.doi.org/10.1139/o89-031.

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The properties and characteristics of oligonucleotide adaptors for use in a simplified procedure for the construction of cDNA and genomic DNA libraries are described. The adaptors are suitable for joining to blunt ended cDNA or sheared genomic DNA, and then to the cohesive ends of restriction sites in vectors. Each adaptor consists of two oligonucleotides with complementary but nonpalindromic sequences that include an internal restriction site, a 5′ phosphorylated blunt end, and an overlapping or staggered 5′ hydroxylated end corresponding to a restriction endonuclease site in a vector of choi

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9

Zhang, Xingli, Chong Teng, Kaidi Lyu, Shanhua Lyu, and Yinglun Fan. "‘Two in One’ Cloning Vector Applied for Blunt-End and T-A Cloning with One-Step Digestion–Ligation and Screening of Positive Recombinants by Unaided Eyes." Current Issues in Molecular Biology 47, no. 1 (2024): 17. https://doi.org/10.3390/cimb47010017.

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To clone DNA sequences quickly and precisely into plasmids is essential for molecular biology studies. Some cloning vectors have been developed for the cloning of PCR products, including blunt-end and T-A cloning. However, different plasmids are required for the cloning of PCR products with blunt ends and 3′ A overhang ends. Here, a novel cloning vector, pYFRed, which is based on the pUC19 backbone, has emerged and can be applied in both blunt-end and T-A cloning. PCR products can be cloned into the pYFRed by a one-step digestion–ligation reaction in a tube. The endonuclease recognition sequen

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10

Koesters, Robert, and Magnus von Knebel Doeberitz. "Improved Blunt-End Cloning by Replacing EcoRV with Eco32I." BioTechniques 32, no. 6 (2002): 1244–46. http://dx.doi.org/10.2144/02326bm05.

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11

Green, Michael R., and Joseph Sambrook. "Cloning Polymerase Chain Reaction (PCR) Products: Blunt-End Cloning." Cold Spring Harbor Protocols 2021, no. 4 (2021): pdb.prot101287. http://dx.doi.org/10.1101/pdb.prot101287.

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12

Leroy, Piet. "Medical errors: the importance of the bullet’s blunt end." European Journal of Pediatrics 170, no. 2 (2010): 251–52. http://dx.doi.org/10.1007/s00431-010-1283-7.

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13

Yang, Shucai, Shuai Su, Xianli Liu, and Pei Han. "Study on Milling Temperature of Titanium Alloy with Micro-Textured Ball End Milling Cutter under Radius of Blunt Edge." Applied Sciences 10, no. 2 (2020): 587. http://dx.doi.org/10.3390/app10020587.

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A high temperature is produced in the process of precision milling of titanium alloy, and the cutting temperature can be effectively reduced by placing a micro-texture on the tool surface. In order to study the milling temperature of micro-textured ball-end milling cutter in milling titanium alloy under the combined action of a blunt radius with different edges and a micro-texture with different parameters, a new method based on micro-element theory and the generation and transmission of cutting heat has been established. At the same time, the influence of different radii of blunt edges on the

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14

Zhang, Kai, and Shu Cai Wang. "Determination of the Box-Counting Fractal Dimension of Pore Distribution in Eggshell Based on Scanning Electron Microscopy Image Analysis." Advanced Materials Research 341-342 (September 2011): 776–79. http://dx.doi.org/10.4028/www.scientific.net/amr.341-342.776.

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The fractal characteristics of pore structures were investigated using duck eggshells as an object of study. The images of the sharp end, the equator and the blunt end of the eggshells were acquired by using scanning electronic microscope. The image processing operations were conducted for the preprocessing and the image converted into binary image. Then we developed programs based on the principle of changing megascopic degree and applied them to plot thelgN(δ)~lg(δ).The relationship betweenlgN(δ)andlg(δ)maintained linearity over a range [2,64] pixels. Therefore, the fractal dimensions of por

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15

Wiaderkiewicz, Ryszard, and Adolfo Ruiz-Carrillo. "Mismatch and blunt to protruding-end joining by DNA ligases." Nucleic Acids Research 15, no. 19 (1987): 7831–48. http://dx.doi.org/10.1093/nar/15.19.7831.

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16

Catchpole, Ken, and Douglas Wiegmann. "Understanding safety and performance in the cardiac operating room: from ‘sharp end’ to ‘blunt end’." BMJ Quality & Safety 21, no. 10 (2012): 807–9. http://dx.doi.org/10.1136/bmjqs-2012-001135.

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17

Girin, Hazarika, Dutta Rupam, Prakash Saikia Deep, Malakar Dipika, Hazorika Mousumi, and Bora Mridusmita. "A comprehensive exploration of restriction enzymes and their applications in molecular biology: A review." World Journal of Advanced Research and Reviews 21, no. 1 (2024): 2399–404. https://doi.org/10.5281/zenodo.13370810.

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Restriction enzymes, derived from bacteria, serve as indispensable tools in molecular biology, enabling precise manipulation of DNA with specificity. These enzymes recognize specific DNA sequences, cleaving the DNA strand at those designated sites. Two types, blunt-end cutters, and sticky-end producers, offer distinct advantages and disadvantages. In molecular biology, restriction enzymes find diverse applications, prominently in gene cloning, facilitating the insertion of foreign DNA into host organisms. Additionally, they play a vital role in DNA sequencing, DNA fingerprinting, and other tec

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18

Purayil, Sreejayan Meethale, and Arun Suthan. "Duodenal injuries in blunt trauma abdomen: a retrospective analysis." International Surgery Journal 6, no. 9 (2019): 3238. http://dx.doi.org/10.18203/2349-2902.isj20194058.

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Background: Blunt trauma to abdomen very rarely produces isolated duodenal injuries and are to the tune of 3-5%. They are associated with high morbidity and mortality because of the diagnostic delay and missed diagnosis. The aim of this study is to discuss the mode of presentation, investigations and treatment options of isolated duodenal injuries in blunt trauma abdomen.Methods: Retrospective analysis of patients with blunt trauma abdomen who presented to surgical department at government medical college, Kozhikode, Kerala was done. Those who sustained isolated duodenal injuries from January

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19

Gallagher, John J. "Management of Blunt Pulmonary Injury." AACN Advanced Critical Care 25, no. 4 (2014): 375–86. http://dx.doi.org/10.4037/nci.0000000000000059.

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Thoracic injuries account for 25% of all civilian deaths. Blunt force injuries are a subset of thoracic injuries and include injuries of the tracheobronchial tree, pleural space, and lung parenchyma. Early identification of these injuries during initial assessment and resuscitation is essential to reduce associated morbidity and mortality rates. Management of airway injuries includes definitive airway control with identification and repair of tracheobronchial injuries. Management of pneumothorax and hemothorax includes pleural space drainage and control of ongoing hemorrhage, along with monito

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20

Pastwa, Elzbieta, Tomasz Poplawski, Agnieszka Czechowska, Mariusz Malinowski, and Janusz Blasiak. "Non-homologous DNA End Joining Repair in Normal and Leukemic Cells Depends on the Substrate Ends." Zeitschrift für Naturforschung C 60, no. 5-6 (2005): 493–500. http://dx.doi.org/10.1515/znc-2005-5-619.

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Double-strand breaks (DSBs) are the most serious DNA damage which, if unrepaired or misrepaired, may lead to cell death, genomic instability or cancer transformation. In human cells they can be repaired mainly by non-homologous DNA end joining (NHEJ). The efficacy of NHEJ pathway was examined in normal human lymphocytes and K562 myeloid leukemic cells expressing the BCR/ABL oncogenic tyrosine kinase activity and lacking p53 tumor suppressor protein. In our studies we employed a simple and rapid in vitro DSB end joining assay based on fluorescent detection of repair products. Normal and cancer

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21

Ucar, Adem, Aghakishi Yahyayev, Ayaz Agayev, et al. "Severe Spasm of the Renal Artery after Blunt Abdominal Trauma Simulating End-Organ Infarction." Case Reports in Medicine 2010 (2010): 1–3. http://dx.doi.org/10.1155/2010/207152.

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Traumatic occlusion of the renal artery is a serious injury. Management differs according to the grade of injury. In most circumstances, emergency surgical revascularization or endovascular intervention is required. We describe the case of a child with multiorgan injuries and spasm of the main renal artery after blunt trauma simulating arterial occlusion or end-organ infarction.

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22

Tinner, C., M. Odermatt, and P. Villiger. "Rare Case of Large Bowel Injury due to Direct Blunt Trauma to a Preexisting Femoral Hernia." Case Reports in Surgery 2017 (2017): 1–4. http://dx.doi.org/10.1155/2017/5308027.

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We report a case of an 85-year-old man with a known asymptomatic left femoral hernia who was admitted to the emergency ward a few hours after falling from a bicycle and suffering from blunt trauma of the handlebar to the left inguinal region. The clinical findings and a computed tomography (CT) scan detecting free air in the femoral hernia sac suggested bowel perforation. Emergency laparotomy 6 hours after the incident confirmed a tear of the sigmoid colon accompanied by free blood and faeces in the left inguinal region of the abdomen. A segmental sigmoid resection and a primary end-to-end col

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23

Nakahara, Shinji, Tetsuya Matsuoka, Masato Ueno, Yasuaki Mizushima, Masao Ichikawa, and Junichiro Yokota. "Extremity Injuries as Predictors of Emergency Care Resource Needs among Blunt Trauma Patients in Japan." American Surgeon 80, no. 2 (2014): 197–203. http://dx.doi.org/10.1177/000313481408000231.

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This study aimed to exhaustively examine associations between prehospital variables and emergency care resource needs among blunt trauma patients. The study included blunt trauma patients aged 15 years or older who were admitted to a tertiary care medical center in Osaka, Japan, from January 2005 to December 2009. The primary end point was a composite measure of overall emergency care resource needs. Predictive variables were easily detectable upper and lower extremity injuries. A multivariate logistic regression model was used to identify associations between the predictive variables and the

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24

Scott, William W., Steven Sharp, Stephen A. Figueroa, et al. "Clinical and radiological outcomes following traumatic Grade 3 and 4 vertebral artery injuries: a 10-year retrospective analysis from a Level I trauma center. The Parkland Carotid and Vertebral Artery Injury Survey." Journal of Neurosurgery 122, no. 5 (2015): 1202–7. http://dx.doi.org/10.3171/2014.9.jns1461.

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OBJECT Grade 3 and 4 blunt vertebral artery (VA) injuries may carry a different natural course from that of lower-grade blunt VA injuries. Proper screening, management, and follow-up of these injuries remain controversial. Grade 3 and 4 blunt VA injuries were analyzed to define their natural history and establish a rational management plan based on lesion progression and cerebral infarction. METHODS A retrospective review of a prospectively maintained database of all blunt traumatic carotid and vertebral artery injuries from August 2003 to April 2013 was performed, and Grade 3 and 4 blunt VA i

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25

Oh, Man Hwan, Je Chul Lee, Jungmin Kim, Chul Hee Choi, and Kyudong Han. "Simple Method for Markerless Gene Deletion in Multidrug-Resistant Acinetobacter baumannii." Applied and Environmental Microbiology 81, no. 10 (2015): 3357–68. http://dx.doi.org/10.1128/aem.03975-14.

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ABSTRACTThe traditional markerless gene deletion technique based on overlap extension PCR has been used for generating gene deletions in multidrug-resistantAcinetobacter baumannii. However, the method is time-consuming because it requires restriction digestion of the PCR products in DNA cloning and the construction of new vectors containing a suitable antibiotic resistance cassette for the selection ofA. baumanniimerodiploids. Moreover, the availability of restriction sites and the selection of recombinant bacteria harboring the desired chimeric plasmid are limited, making the construction of

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26

Curry, John D., Lydia Li та Mark S. Schlissel. "Quantification of Jκ signal end breaks in developing B cells by blunt-end linker ligation and qPCR". Journal of Immunological Methods 296, № 1-2 (2005): 19–30. http://dx.doi.org/10.1016/j.jim.2004.10.003.

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27

Safin, R. R., O. G. Anisimov, and A. A. Nazipov. "New method of epidural anesthesia in closed blunt chest injury." Kazan medical journal 82, no. 1 (2021): 18–21. http://dx.doi.org/10.17816/kazmj70887.

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The thoracic epidural anesthesia is the most suitable method for the treatment of the closed blunt chest injury but there is probability of the spinal cord injuiry. The original method combining the efficiency of classic thoracic epidural anesthesia with safety and simplicity of lumbar epidural anesthesia is suggested. This method is based on the postulates of the molecular hydrokinetic theory. Two catheters ends are disposed in lumbar epidural space in distance about two inches between them. Through one catheters end the anesthetic solution and through others end the 0,9% saline are injected

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28

Koehl, Alexander, Eckhard Friauf, and Hans Gerd Nothwang. "Efficient Cloning of SAGE Tags by Blunt-End Ligation of Polished Concatemers." BioTechniques 34, no. 4 (2003): 692–94. http://dx.doi.org/10.2144/03344bm01.

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29

Grupa, Ulrich, Korbinian Liebl, and Martin Zacharias. "Orientation Dependence of DNA Blunt-End Stacking Studied by Free-Energy Simulations." Journal of Physical Chemistry B 125, no. 51 (2021): 13850–57. http://dx.doi.org/10.1021/acs.jpcb.1c07829.

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30

Kolganova, Natalia A., Vadim A. Vasiliskov, Viktoriya E. Kuznetsova, et al. "Factors Affecting the Tailing of Blunt End DNA with Fluorescent Pyrimidine dNTPs." Molecular Biotechnology 60, no. 12 (2018): 879–86. http://dx.doi.org/10.1007/s12033-018-0124-1.

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31

Yang, Yosheph, Ikhyun Kim, and Gisu Park. "Evaluation of blunt body velocity gradient at the shock tube end-wall." Acta Astronautica 170 (May 2020): 570–76. http://dx.doi.org/10.1016/j.actaastro.2020.02.032.

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32

Davis, Janet L. "The Blunt End: Surgical Challenges of Gene Therapy for Inherited Retinal Diseases." American Journal of Ophthalmology 196 (December 2018): xxv—xxix. http://dx.doi.org/10.1016/j.ajo.2018.08.038.

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33

Kanayama, Naoki, Satomi Kishi, Tohru Takarada, and Mizuo Maeda. "Photo-switching of blunt-end stacking between DNA strands immobilized on gold nanoparticles." Chemical Communications 56, no. 93 (2020): 14589–92. http://dx.doi.org/10.1039/d0cc05085g.

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34

Capote, Allan, Andrew Michael, Jorge Almodovar, Patricia Chan, Ruby Skinner, and Maureen Martin. "Emergency Department Thoracotomy: Too Little, Too Much, or Too Late." American Surgeon 79, no. 10 (2013): 982–86. http://dx.doi.org/10.1177/000313481307901005.

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Emergency department thoracotomy (EDT) is a dramatic lifesaving procedure demanding timely surgical intervention, technical expertise, and coordinated resuscitation efforts. Inappropriate use is costly and futile. All patients admitted to a Level II trauma center who underwent EDT from January 2003 to July 2012 were studied. The primary end point was appropriateness of EDT. Secondary end points were staff exposure, survival, and return to normal function. Eighty-seven patients including 59 patients with penetrating wounds had a mean loss of vital signs (LOV) 11.6 610.6 minutes and Injury Sever

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35

Lin, Huawei, Zeyuan Zhang, Longlong Zou, and Chunlei Yang. "Deep Learning-Based Detection and Classification of Blunt Abdominal Trauma." International Journal of Computer Science and Information Technology 6, no. 1 (2025): 37–45. https://doi.org/10.62051/ijcsit.v6n1.05.

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Traumatic injuries are the leading cause of death in the first four decades of human life and represent a major global public health issue. It is estimated that over 5 million people die from traumatic injuries each year worldwide. Among these, blunt abdominal trauma (BAT) accounts for approximately 75% of all blunt injuries, making it the most common type. However, in the early stages, there may be few or no obvious signs indicating serious intra-abdominal injuries, which makes assessment particularly challenging and requires a high index of clinical suspicion. In such cases, accurate and tim

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36

Phillips, J. W., and W. F. Morgan. "Illegitimate recombination induced by DNA double-strand breaks in a mammalian chromosome." Molecular and Cellular Biology 14, no. 9 (1994): 5794–803. http://dx.doi.org/10.1128/mcb.14.9.5794-5803.1994.

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We examined DNA double-strand-break-induced mutations in the endogenous adenine phosphoribosyl-transferase (APRT) gene in cultured Chinese hamster ovary cells after exposure to restriction endonucleases. PvuII, EcoRV, and StuI, all of which produce blunt-end DNA double-strand breaks, were electroporated into CHO-AT3-2 cells hemizygous at the APRT locus. Colonies of viable cells containing mutations at APRT were expanded, and the mutations that occurred during break repair were analyzed at the DNA sequence level. Restriction enzyme-induced mutations consisted of small deletions of 1 to 36 bp, i

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37

Phillips, J. W., and W. F. Morgan. "Illegitimate recombination induced by DNA double-strand breaks in a mammalian chromosome." Molecular and Cellular Biology 14, no. 9 (1994): 5794–803. http://dx.doi.org/10.1128/mcb.14.9.5794.

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We examined DNA double-strand-break-induced mutations in the endogenous adenine phosphoribosyl-transferase (APRT) gene in cultured Chinese hamster ovary cells after exposure to restriction endonucleases. PvuII, EcoRV, and StuI, all of which produce blunt-end DNA double-strand breaks, were electroporated into CHO-AT3-2 cells hemizygous at the APRT locus. Colonies of viable cells containing mutations at APRT were expanded, and the mutations that occurred during break repair were analyzed at the DNA sequence level. Restriction enzyme-induced mutations consisted of small deletions of 1 to 36 bp, i

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38

Tong, Xin, Shoumeng Wang, Xiyue Wang, and Qiang Qu. "Analysis of Surface Characteristics of Titanium Alloy Milling with Ball-End Milling Cutters Based on Mesoscopic Geometric Features." Coatings 14, no. 6 (2024): 670. http://dx.doi.org/10.3390/coatings14060670.

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In order to further reduce the height of burrs on the surface of the workpiece when milling titanium alloy with ball-end milling cutters and optimize the quality of the workpiece, this article takes the mesoscopic geometric feature of ball-end milling cutters as the research object and establishes the theoretical relationship between the mesoscopic geometric feature parameters and the height of titanium alloy burrs during milling. A milling test platform was built to explore the influence of micro-texture, blunt edge single factor, and their interaction with cutting parameters on the force-the

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39

Gustafsson, Karl Erik. "End-game Strategies in the Swedish National Tabloid Industry." Nordicom Review 29, no. 2 (2008): 325–34. http://dx.doi.org/10.1515/nor-2017-0194.

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Abstract Based on decades of comparative research on the national tabloid industry in the Nordic countries, a forecast is made, based on the product life cycle (PLC) theory, that the Swedish national tabloid industry will decline. As PLC theory is a blunt forecasting instrument, every precaution is taken; still a forecast is made that the No.2 tabloid will lose the present endgame to the market leader and disappear in 5-10 years time. End-game strategies as well as exit barriers are discussed in detail. The conclusions are valid for the national tabloid industries in the other Nordic countries

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40

Rashid, M. R. Abdul, M. R. Adli Azam, H. Hairulfaizi, et al. "Right Main Bronchus Transection Post Blunt Trauma: A Case Report." Scholars Journal of Medical Case Reports 9, no. 6 (2021): 645–47. http://dx.doi.org/10.36347/sjmcr.2021.v09i06.008.

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Tracheobronchial Injury is a relatively uncommon condition which is associated with high mortality. Majority occurring from blunt chest trauma sustained from deceleration injury in road traffic accidents. We present a case of a 17-year-old young male who presented in the acute setting of post motorcycle accident with respiratory distress, subcutaneous emphysema, right pneumothorax and fracture of posterior right 8th-9th ribs. CT shows right main bronchus anterior wall tear, with extensive subcutaneous emphysema, pneumomediastinum, bilateral pneumothorax and pneumoperitoneum. Thoracotomy confir

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41

Winegar, Richard A., and R. Julian Preston. "The induction of chromosome aberrations by restriction endonucleases that produce blunt-end or cohesive-end double-strand breaks." Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 197, no. 1 (1988): 141–49. http://dx.doi.org/10.1016/0027-5107(88)90150-9.

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42

Chen, Kaifang, Sheng Yao, Fan Yang, et al. "Minimally Invasive Screw Fixation of Unstable Pelvic Fractures Using the “Blunt End” Kirschner Wire Technique Assisted by 3D Printed External Template." BioMed Research International 2019 (October 24, 2019): 1–9. http://dx.doi.org/10.1155/2019/1524908.

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Objective. This study aimed to determine the accuracy and safety of the “blunt end” Kirschner wire (KW) technique for the minimally invasive treatment of unstable pelvic fractures with the assistance of a 3D printed external template. Methods. Clinical data of 28 patients with unstable pelvic fractures between January 2016 and January 2018 were retrospectively analyzed. There were 6 cases of B1, 10 of B2, 8 of C1, and 4 of C2 fractures, all of which received surgical treatment. The “blunt end” KW technique with a 3D template was adopted for the minimally invasive placement of the iliosacral (I

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43

Du, Weikang, and Spyros A. Kinnas. "A flow separation model for hydrofoil, propeller and duct sections with blunt trailing edges." Journal of Fluid Mechanics 861 (December 19, 2018): 180–99. http://dx.doi.org/10.1017/jfm.2018.859.

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The panel method does not apply to hydrofoils, propellers and ducts with blunt trailing edges due to the flow separation downstream. In this paper, a model is proposed to represent the flow separation with an extension, and a low-order panel method coupled with a boundary layer solver is used. The criteria of zero lift and zero moment are adopted to determine the end of the extension zone, and flow separation criteria are used to determine the starting points on either side of the section. The model is applied to hydrofoil, bare duct and ducted propeller sections with blunt trailing edges. The

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44

Tong, Xin, Xianli Liu, and Song Yu. "Support vector regression and genetic-algorithm-based multiobjective optimization of mesoscopic geometric characteristic parameters of ball-end milling tool." Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture 234, no. 10 (2020): 1333–45. http://dx.doi.org/10.1177/0954405420911528.

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The poor machinability of titanium alloys results in the serious wear of the rake face of a ball-end milling tool. Previous studies indicated that the mesoscopic geometric characteristics of the tool can effectively improve the wear resistance. Therefore, in this thesis, a milling force model and a milling temperature model of a ball-end milling tool were established to verify the effect of the blunt and negative chamfer tool edges. Setting up a test platform for milling titanium alloy, the influence of mesoscopic geometric characteristic parameters on cutting performance of the ball-end milli

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45

Nelson, A. D. L., and D. E. Shippen. "Blunt-ended telomeres: an alternative ending to the replication and end protection stories." Genes & Development 26, no. 15 (2012): 1648–52. http://dx.doi.org/10.1101/gad.199059.112.

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46

Sánchez, Antonio, Mónica Bullejos, Miguel Burgos, Rafael Jiménez, and Rafael Diaz de la Guardia. "An alternative to blunt-end ligation for cloning DNA fragments with incompatible ends." Trends in Genetics 12, no. 2 (1996): 44. http://dx.doi.org/10.1016/s0168-9525(96)90051-7.

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47

Zheleznaya, Lyudmila, Sergey Shiryaev, Elena Zheleznyakova, Nadezhda Matvienko, and Nicholas Matvienko. "R.Ssp D5I is a neoschizomer of Hph I producing blunt end DNA fragments." FEBS Letters 448, no. 1 (1999): 38–40. http://dx.doi.org/10.1016/s0014-5793(99)00330-0.

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Gao, Song, Jiannan Zhang, Tianjin Miao, et al. "A Simple and Convenient Sticky/Blunt-End Ligation Method for Fusion Gene Construction." Biochemical Genetics 53, no. 1-3 (2015): 42–48. http://dx.doi.org/10.1007/s10528-015-9669-x.

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Clark, James M., Catherine M. Joyce, and G. Peter Beardsley. "Novel blunt-end addition reactions catalyzed by DNA polymerase I of Escherichia coli." Journal of Molecular Biology 198, no. 1 (1987): 123–27. http://dx.doi.org/10.1016/0022-2836(87)90462-1.

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Refaely, Yael, Leonid Koyfman, Michael Friger, et al. "Clinical Outcome of Urgent Thoracotomy in Patients with Penetrating and Blunt Chest Trauma: A Retrospective Survey." Thoracic and Cardiovascular Surgeon 66, no. 08 (2017): 686–92. http://dx.doi.org/10.1055/s-0037-1608899.

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Abstract:

Introduction In contrast to an emergency department of thoracotomy (EDT), an urgent thoracotomy (UT) is defined as a surgical thoracic intervention performed in the operating room within the first 48 hours of the patient's intensive care unit (ICU) stay. The factors affecting survival after UT are not fully understood. In this study, we retrospectively analyzed the clinical data and outcome of patients with blunt and penetrating chest injuries who underwent UT. Methods All adult patients who had blunt or penetrating chest trauma and who underwent UT, were included in the study. All data were c

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