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Journal articles on the topic 'Bottom-up enzymatic synthesis'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

Ye, Tai, Chunying Li, Chen Su, et al. "Enzymatic polymerization of poly(thymine) for the synthesis of copper nanoparticles with tunable size and their application in enzyme sensing." Chemical Communications 51, no. 41 (2015): 8644–47. http://dx.doi.org/10.1039/c5cc01517k.

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2

Bolm, Carsten, and José G. Hernández. "From Synthesis of Amino Acids and Peptides to Enzymatic Catalysis: A Bottom-Up Approach in Mechanochemistry." ChemSusChem 11, no. 9 (2018): 1410–20. http://dx.doi.org/10.1002/cssc.201800113.

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3

Ochiai, Hirofumi, Masashi Ohmae, Tomonori Mori, and Shiro Kobayashi. "Bottom-Up Synthesis of Hyaluronan and Its Derivatives via Enzymatic Polymerization: Direct Incorporation of an Amido Functional Group." Biomacromolecules 6, no. 2 (2005): 1068–84. http://dx.doi.org/10.1021/bm049280r.

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4

Zhong, Chao, Krisztina Zajki-Zechmeister, and Bernd Nidetzky. "Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material." Carbohydrate Polymers 260 (February 11, 2021): 117772. https://doi.org/10.1016/j.carbpol.2021.117772.

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<strong>Abstract</strong> Nanoparticle-polymer composites are important functional materials but structural control of their assembly is challenging. Owing to its crystalline internal structure and tunable nanoscale morphology, cellulose is promising polymer scaffold for templating such composite materials. Here, we show bottom-up synthesis of reducing end thiol-modified cellulose chains by iterative bi-enzymatic β-1,4-glycosylation of 1-thio-β-D-glucose (10 mM), to a degree of polymerization of &sim;8 and in a yield of &sim;41% on the donor substrate (α-D-glucose 1-phosphate,

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5

Zhong, Chao, and Bernd Nidetzky. "Precision synthesis of reducing-end thiol-modified cellulose enabled by enzyme selection." Polymer Journal 54 (December 3, 2021): 551–60. https://doi.org/10.1038/s41428-021-00599-4.

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<strong>Abstract</strong> Enzyme-catalyzed iterative <em>β</em>-1,4-glycosylation of <em>β</em>-glycosides is promising for bottom-up polymerization of reducing-end-modified cello-oligosaccharide chains. Self-assembly of the chains from solution yields crystalline nanocellulose materials with properties that are tunable by the glycoside group used. Cellulose chains with a reducing-end thiol group are of interest to install a controllable pattern of site-selective modifications into the nanocellulose material. Selection of the polymerizing enzyme (cellodextrin phosphorylase;

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6

Zhong, Chao, Krisztina Zajki-Zechmeister, and Bernd Nidetzky. "Reducing end thiol-modified nanocellulose: Bottom-up enzymatic synthesis and use for templated assembly of silver nanoparticles into biocidal composite material." Carbohydrate Polymers 260 (May 2021): 117772. http://dx.doi.org/10.1016/j.carbpol.2021.117772.

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7

Reshma, Reshma, and Bhanushree Gupta. "Carbon Dots in Biomedical Applications: A Review of Their Interaction with Serum Albumins, Antidepressant Agents, and Enzymatic Systems." Journal of Ravishankar University (PART-B) 38, no. 1 (2025): 172–88. https://doi.org/10.52228/jrub.2025-38-1-12.

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Carbon dots (CDs) are a type of carbon-based nanoparticles that can be categorized into subtypes based on their structure and morphology. These nanoparticles possess adjustable physical, chemical, and optical characteristics, which can be easily manipulated through simple one-pot synthesis methods. CDs are highly attractive due to their biocompatibility, non-toxic nature, resistance to photobleaching and chemical degradation, and cost-effectiveness, making them suitable for a wide array of applications. Their synthesis can be carried out using two main strategies: (i) top-down approaches and (

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8

Zhong, Chao, Christina Ukowitz, Konrad Domig, and Bernd Nidetzky. "Short-chain cello-oligosaccharides: Intensification and scale-up of their enzymatic production and selective growth promotion among probiotic bacteria." Journal of Agricultural and Food Chemistry 68, no. 32 (2020): 8557–67. https://doi.org/10.1021/acs.jafc.0c02660.

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<strong>Abstract</strong> Short-chain cello-oligosaccharides (COS; degree of polymerization, DP≤6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show intensification and scale up (20 mL; gram scale) of COS production to 93 g/L product and in 82 mol % yield from sucrose (0.5 M). The COS were comprised of DP 3 (33 wt %), DP 4 (34 wt %), DP 5 (24 wt %) and DP 6 (9 wt %) and involved minimum loss (≤10 mol %) to insoluble fractions. After isolation (≥95% purity; ≥90% yie

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9

Bußkamp, Holger, Sascha Keller, Marta Robotta, Malte Drescher, and Andreas Marx. "A new building block for DNA network formation by self-assembly and polymerase chain reaction." Beilstein Journal of Organic Chemistry 10 (May 7, 2014): 1037–46. http://dx.doi.org/10.3762/bjoc.10.104.

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The predictability of DNA self-assembly is exploited in many nanotechnological approaches. Inspired by naturally existing self-assembled DNA architectures, branched DNA has been developed that allows self-assembly to predesigned architectures with dimensions on the nanometer scale. DNA is an attractive material for generation of nanostructures due to a plethora of enzymes which modify DNA with high accuracy, providing a toolbox for many different manipulations to construct nanometer scaled objects. We present a straightforward synthesis of a rigid DNA branching building block successfully used

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10

Zhong, Chao, and Bernd Nidetzky. "Precision synthesis of reducing-end thiol-modified cellulose enabled by enzyme selection." Polymer Journal 54, no. 4 (2021): 551–60. http://dx.doi.org/10.1038/s41428-021-00599-4.

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AbstractEnzyme-catalyzed iterative β-1,4-glycosylation of β-glycosides is promising for bottom-up polymerization of reducing-end-modified cello-oligosaccharide chains. Self-assembly of the chains from solution yields crystalline nanocellulose materials with properties that are tunable by the glycoside group used. Cellulose chains with a reducing-end thiol group are of interest to install a controllable pattern of site-selective modifications into the nanocellulose material. Selection of the polymerizing enzyme (cellodextrin phosphorylase; CdP) was pursued here to enhance the synthetic precisio

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11

Kim, Chang Hyun, DongHwan Oh, Hwakyoung Seo, et al. "Oxidizing Gas Reverses Thermal Ripening of Ni Particles into Single Atom for Solar-Driven Biocatalytic Hydrocarbon Oxygenation." ECS Meeting Abstracts MA2024-02, no. 59 (2024): 3957. https://doi.org/10.1149/ma2024-02593957mtgabs.

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Single atoms (SAs), or individual heteroatoms have garnered much interest due to their potential on the revolution of the chemical and energy industrial fields with exceptional metal utilization and unforeseen catalytic advantages from non-ensemble sites. Typically, SAs are synthesized through bottom-up methodologies that involve the ligation between support materials and metal salts. Conversely, there are only a few reports on top-down methods, and even these are primarily focused on precious group elements supported by metal oxide. Coarsening of nanoparticles happens at high temperatures due

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12

Suneth P. Rajapaksha and M.A.A. Aqeel. "Enzyme Related Synthesis of Lipids and Fabrication of Planar Lipid Membranes." Vidyodaya Journal of Science 1, s1 (2023). http://dx.doi.org/10.31357/vjs.v1is1.6713.

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Biological membranes are structurally and functionally complex supramolecular assemblies. The intricate complexity itself poses a significant barrier in investigating the constituents, components, and their structure-activity relationships. Scientists employ twosynthetic methods to reduce complexity: the top-down approach and the bottom-up approach. The top-down synthetic approach involves genome reduction of the living cell, leading to a collateral reduction in membrane complexity. In contrast, the bottom-upsynthetic approach like chemical and enzymatic synthesis engages assembly of non-livin

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13

Zhong, Chao, and Bernd Nidetzky. "Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials." Biotechnology Advances 51 (September 20, 2020). https://doi.org/10.1016/j.biotechadv.2020.107633.

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<strong>Abstract</strong> Cellulose-based materials are produced industrially in countless varieties via top-down processing of natural lignocellulose substrates. By contrast, cellulosic materials are only rarely prepared via bottom up synthesis and oligomerization-induced self-assembly of cellulose chains. Building up a cellulose chain via precision polymerization is promising, however, for it offers tunability and control of the final chemical structure. Synthetic cellulose derivatives with programmable material properties might thus be obtained. Cellodextrin phosphorylase (CdP; EC 2.4.1.49)

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14

Klimacek, Mario, Chao Zhong та Bernd Nidetzky. "Kinetic modeling of phosphorylase-catalyzed iterative β-1,4-glycosylation for degree of polymerization-controlled synthesis of soluble cello-oligosaccharides." Biotechnology for Biofuels 14 (10 червня 2021). https://doi.org/10.1186/s13068-021-01982-2.

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<strong>Abstract</strong> <strong>Background: </strong>Cellodextrin phosphorylase (CdP; EC 2.4.1.49) catalyzes the iterative β-1,4-glycosylation of cellobiose using α-D-glucose 1-phosphate as the donor substrate. Cello-oligosaccharides (COS) with a degree of polymerization (DP) of up to 6 are soluble while those of larger DP self-assemble into solid cellulose material. The soluble COS have attracted considerable attention for their use as dietary fibers that offer a selective prebiotic function. An efficient synthesis of soluble COS requires good control over the DP of the products

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15

Schwaiger, Katharina N., Alena Voit, Birgit Wiltschi, and Bernd Nidetzky. "Engineering cascade biocatalysis in whole cells for bottom-up synthesis of cello-oligosaccharides: flux control over three enzymatic steps enables soluble production." Microbial Cell Factories 21, no. 1 (2022). http://dx.doi.org/10.1186/s12934-022-01781-w.

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Abstract Background Soluble cello-oligosaccharides (COS, β‐1,4‐D‐gluco‐oligosaccharides with degree of polymerization DP 2–6) have been receiving increased attention in different industrial sectors, from food and feed to cosmetics. Development of large-scale COS applications requires cost-effective technologies for their production. Cascade biocatalysis by the three enzymes sucrose-, cellobiose- and cellodextrin phosphorylase is promising because it enables bottom-up synthesis of COS from expedient substrates such as sucrose and glucose. A whole-cell-derived catalyst that incorporates the requ

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16

Löffler, Philipp M. G., Anders Højgaard Hansen, Oliver Ries, et al. "Lipidated Polyaza Crown Ethers as Membrane Anchors for DNA-Controlled Content Mixing between Liposomes." Scientific Reports 9, no. 1 (2019). http://dx.doi.org/10.1038/s41598-019-49862-y.

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Abstract The ability to manipulate and fuse nano-compartmentalized volumes addresses a demand for spatiotemporal control in the field of synthetic biology, for example in the bottom-up construction of (bio)chemical nanoreactors and for the interrogation of enzymatic reactions in confined space. Herein, we mix entrapped sub-attoliter volumes of liposomes (~135 nm diameter) via lipid bilayer fusion, facilitated by the hybridization of membrane-anchored lipidated oligonucleotides. We report on an improved synthesis of the membrane-anchor phosphoramidites that allows for a flexible choice of lipop

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17

Schwaiger, Katharina N., and Bernd Nidetzky. "Continuous process technology for bottom-up synthesis of soluble cello-oligosaccharides by immobilized cells co-expressing three saccharide phosphorylases." Microbial Cell Factories 21, no. 265 (2022). https://doi.org/10.1186/s12934-022-01984-1.

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<strong>Abstract</strong> <strong>Background.</strong> Continuous processing with enzyme reuse is a well-known engineering strategy to enhance the efficiency of biocatalytic transformations for chemical synthesis. In one-pot multistep reactions, continuous processing offers the additional benefit of ensuring constant product quality via control of the product composition. Bottom-up production of cello-oligosaccharides (COS) involves multistep iterative b1,4-glycosylation of glucose from sucrose catalysed by sucrose (ScP), cellobiose (CbP) and cellodextrin phosphorylase (CdP). Degree of polymer

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18

Zhang, Xiaoyu, Xinyu Wu, Jian Zhang, Huiyan Xu, and Xin Yu. "Recent progress in graphitic carbon nitride-based materials for antibacterial applications: synthesis, mechanistic insights, and utilization." Microstructures 4, no. 2 (2024). http://dx.doi.org/10.20517/microstructures.2023.77.

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Recent breakthroughs in graphitic carbon nitride (g-C3N4)-based materials have catalyzed the development of highly effective antibacterial strategies. This comprehensive review delves into the synthesis, mechanistic insights, and applications of g-C3N4 in the realm of antibacterial research. The introduction first highlights the importance of antibacterial materials, emphasizing the urgent need for innovative solutions in the face of bacterial infections and the escalating challenges posed by antibiotic resistance. Continuing, the structural attributes and distinctive characteristics of g-C3N4

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19

Pandya, Pranav, Thomas J. Webster, and Sougata Ghosh. "Nanobioprospecting of photoautotrophs for the fabrication of quantum dots: mechanism and applications." Frontiers in Chemistry 12 (October 15, 2024). http://dx.doi.org/10.3389/fchem.2024.1458804.

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Quantum dots (QDs), also known as nanoparticle-based fluorescent probes, are luminescent semiconductor particles with a size range of 2–20 nm. The unique optical and electronic capabilities of QDs have led to expanded applications in several fields such as optoelectronics, transistors, sensors, photodetection, catalysis, and medicine. The distinct quantum effects of nanocrystals can be controlled by changing their sizes and shapes using a variety of top-down and bottom-up tactics. QDs were traditionally fabricated using complex, expensive, toxic, and aggressive chemical techniques, which limit

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20

Creed, Magnus, Marta Szulik, Ryan Bia, Chris Tracy, Aman Makaju, and Sarah Franklin. "Abstract P513: Adenylosuccinate Synthase Is A Novel Methylation Target Of Smyd1." Circulation Research 129, Suppl_1 (2021). http://dx.doi.org/10.1161/res.129.suppl_1.p513.

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Among the metabolic shifts in chronic heart failure is a dysregulation of purine metabolism, which has been shown to negatively impact patient outcomes, especially in individuals affected by hypertension, diabetes, and congestive heart failure, via increased serum uric acid levels and cellular oxidative stress. The underlying mechanisms which drive these changes in purine metabolism in the cardiomyocyte and ultimately reactive oxygen species and uric acid accumulation in heart failure patients remain largely unknown. We recently discovered that the methyltransferase Smyd1 interacts with the me

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21

Sarkar, Jagannath. "Genomics of Brevibacterium strains reveal adaptations necessary to acclimatize in different marine environments." June 7, 2024. https://doi.org/10.5281/zenodo.13624149.

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<strong>ABSTRACT</strong> Microbes are ubiquitous throughout the seawater and underlying sediment, which covers the vast region of the earth surface. Strains of different genera under the phylum <em>Actinobacteria</em> are ubiquitous all-over different environments of the earth surfaces. A well-studied aerobic genus, <em>Brevibacterium</em>, under the phylum also found to be omnipresent in both marine and terrestrial environments, ranging from coastal to open ocean. Which raise question, whether marine strains are phylogenetically distinct from others or not, and what are the special genomic a

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22

Ameku, W.A., V.N. Ataide, E.T. Costab, et al. "A pencil-lead immunosensor for the rapid electrochemical measurement of anti-Diphtheria Toxin antibodies." October 30, 2021. https://doi.org/10.5281/zenodo.5628477.

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<strong>Abstract: </strong>Diphtheria is a vaccine-preventable disease, yet immunization can wane over time to non-protective levels. We have developed a low-cost, miniaturized electroanalytical biosensor to quantify anti-diphtheria toxin (DTx) immunoglobulin G (IgG) antibody to minimize the risk for localized outbreaks. Two epitopes specific to DTx and recognized by antibodies generated post-vaccination were selected to create a bi-epitope peptide, biEP, by synthesizing the epitopes in tandem. The biEP peptide was conjugated to the surface of a pencil-lead electrode (PLE) integrated into a po

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