Academic literature on the topic 'Brainbow strategy'

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Journal articles on the topic "Brainbow strategy"

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Cook, Zoe T., Nicole L. Brockway, Zachary J. C. Tobias, et al. "Combining near-infrared fluorescence with Brainbow to visualize expression of specific genes within a multicolor context." Molecular Biology of the Cell 30, no. 4 (2019): 491–505. http://dx.doi.org/10.1091/mbc.e18-06-0340.

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Fluorescent proteins are a powerful experimental tool, allowing the visualization of gene expression and cellular behaviors in a variety of systems. Multicolor combinations of fluorescent proteins, such as Brainbow, have expanded the range of possible research questions and are useful for distinguishing and tracking cells. The addition of a separately driven color, however, would allow researchers to report expression of a manipulated gene within the multicolor context to investigate mechanistic effects. A far-red or near-infrared protein could be particularly suitable in this context, as thes
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Shen, Fred Y., Margaret M. Harrington, Logan A. Walker, Hon Pong Jimmy Cheng, Edward S. Boyden, and Dawen Cai. "Light microscopy based approach for mapping connectivity with molecular specificity." Nature Communications 11, no. 1 (2020). http://dx.doi.org/10.1038/s41467-020-18422-8.

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Abstract Mapping neuroanatomy is a foundational goal towards understanding brain function. Electron microscopy (EM) has been the gold standard for connectivity analysis because nanoscale resolution is necessary to unambiguously resolve synapses. However, molecular information that specifies cell types is often lost in EM reconstructions. To address this, we devise a light microscopy approach for connectivity analysis of defined cell types called spectral connectomics. We combine multicolor labeling (Brainbow) of neurons with multi-round immunostaining Expansion Microscopy (miriEx) to simultane
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Sakaguchi, Richi, Marcus N. Leiwe, and Takeshi Imai. "Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags." eLife 7 (November 20, 2018). http://dx.doi.org/10.7554/elife.40350.

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The stochastic multicolor labeling method ‘Brainbow’ is a powerful strategy to label multiple neurons differentially with fluorescent proteins; however, the fluorescence levels provided by the original attempts to use this strategy were inadequate. In the present study, we developed a stochastic multicolor labeling method with enhanced expression levels that uses a tetracycline-operator system (Tetbow). We optimized Tetbow for either plasmid or virus vector-mediated multicolor labeling. When combined with tissue clearing, Tetbow was powerful enough to visualize the three-dimensional architectu
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Browne, Tyler J., Kelly M. Smith, Mark A. Gradwell, et al. "Lateral lamina V projection neuron axon collaterals connect sensory processing across the dorsal horn of the mouse spinal cord." Scientific Reports 14, no. 1 (2024). http://dx.doi.org/10.1038/s41598-024-73620-4.

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AbstractSpinal projection neurons (PNs) are defined by long axons that travel from their origin in the spinal cord to the brain where they relay sensory information from the body. The existence and function of a substantial axon collateral network, also arising from PNs and remaining within the spinal cord, is less well appreciated. Here we use a retrograde viral transduction strategy to characterise a novel subpopulation of deep dorsal horn spinoparabrachial neurons. Brainbow assisted analysis confirmed that virally labelled PN cell bodies formed a discrete cell column in the lateral part of
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Dissertations / Theses on the topic "Brainbow strategy"

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Bretonnière, Delphine. "Contrôle temporel de la neurogenèse par la dynamique du cycle cellulaire : de la modélisation à l'analyse expérimentale." Electronic Thesis or Diss., Toulouse 3, 2023. http://www.theses.fr/2023TOU30377.

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Dans les organismes pluricellulaires, le contrôle de l'équilibre prolifération/différenciation des cellules souches/progéniteurs est essentiel au développement et à l'homéostasie des tissus. Une dérégulation de cet équilibre provoque des défauts développementaux, cancers ou défauts de réparation des lésions. Le destin des cellules souches lors de la mitose est contrôlé en partie par la durée du cycle cellulaire. Nos travaux ont montré que dans les progéniteurs neuraux, l'activité de la phosphatase CDC25B, un régulateur du cycle cellulaire, modifie la cinétique du cycle cellulaire et stimule la
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