Dissertations / Theses on the topic 'Branduolinė'
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Apolevič, Jolanta. "The Impact of the Principles of International Environmental Law on Nuclear Law." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140922_141136-97141.
Full textDisertacijoje nagrinėjama tiesioginė ir netiesioginė aplinkos teisės principų įtaka tarptautinei branduolinei teisei ir branduolinės energetikos objektų reguliavimui, siekiant informuoti sprendimus priimančias institucijas ir visuomenę apie pagrindinius iššūkius, kuriuos reikia įveikti siekiant, kad branduolinė energetika kiek įmanoma veiksmingiau prisidėtų įgyvendinant aplinkosauginius tikslus, o aplinka būtų tinkamai apsaugota nuo taikiais tikslais vystomos branduolinės veiklos keliamos tarpvalstybinės žalos rizikos (analizės metu vyrauja ekocentrinis, o ne antropocentrinis požiūris). Analizės objektu pasirinkti tie aplinkos teisės principai, kurie numato gaires tarptautinėms branduolinės teisės normoms plėtotis, nustato gamtos apsaugos režimą (saugiklius) bei tarptautinę atsakomybę, atsirandančią dėl branduolinės energetikos objektų sukelto žalingo poveikio aplinkai (t.y. bendradarbiavimo, darnaus vystymosi, prevencijos, atsargumo, teršėjas moka ir informavimo principai). Nurodžius tarptautinės aplinkos teisės ir branduolinės teisės sąlyčio taškus, toliau nagrinėjamos problemos dėl aplinkos teisės principų raidos ir perspektyvų branduolinės energetikos objektų reglamentavimo srityje, pateikiama susijusi nacionalinių ir tarptautinių ginčų sprendimo institucijų dėl branduolinės energetikos objektų praktika, atsakoma į klausimą, kuris iš minėtų aplinkos teisės principų veikimo modelių – tiesioginis ar netiesioginis – yra veiksmingesnis siekiant įgyvendinti užsibrėžtus... [toliau žr. visą tekstą]
Gaidamavičiūtė, Alina. "Branduolinio tipo ekstremaliųjų situacijų prevencijos ir parengties analizė." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2013~D_20140128_134340-48273.
Full textMaster's thesis analyzed and evaluated nuclear emergency prevention and preparedness measures. The first part is an overview of nuclear safety, prevention and preparedness phases conception of emergency management cycle. It is also examined the nature of technological risk perception and its operating features and the type of nuclear emergency classification according to the INES scale. The second part analyzed the nuclear emergency prevention and preparedness measures and it is given a brief overview of the situation in Lithuania, assessed according to these measures. The third section discussed the research carried out by triangulation method: qualitative and quantitative. Qualitative research revealed the Chernobyl accident prevention and preparedness measures and identified gaps in emergency management process. Quantitative research analyzed public opinion on nuclear safety in Lithuania. Also experts in radiological sphere have given their opinion about radiological safety measures.
Liachovič, Aleksandra. "Ar egzistuoja pareiga juridiniam asmeniui atlyginti žalą kitai valstybei, kai dėl force majeure aplinkybių radioaktyviomis branduolinėmis medžiagomis užteršiami kitos valstybės išoriniai teritoriniai vandenys bei vandenynai?" Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140619_113510-09836.
Full textRecently, the number of cases is rising because of the various hazardous materials contamination around the nature. Most often this occurs because of unexpected natural disasters. For example, due to a strong earthquake that triggered the tsunami, a huge disaster in Japan occurred (Fukushima nuclear power plant case). At that time, a large part of radioactive nuclear materials spread in the open ocean and this breakthrough that is widespread even now still cannot be stopped. It applies not only to the pollution of the ocean, but to all other different countries within outside territorial waters. The question is: Are the affected State has the right to compensation in this case? Then who should pay for damages? What is the legal regulation for such situations? International environmental law is mainly expressed in treaties and other international legal instruments, but it cannot be applied for the marine pollution with nuclear materials because there is no legal regulation in this field. Hereinafter, the problem of deficiency of legal regulation will be examined specifically in the area of oceans, sea borders, and territorial waters pollution by radioactive materials. And finally, there will be explored the issues to whether there is an obligation for a legal entity to compensate the damage to another state when according to the force majeure circumstances the radioactive nuclear materials spread and contaminate other state's outside territorial waters and ocean? The main goal... [to full text]
Mikalauskas, Vytenis. "Radiologinių tyrimo metodų palyginamoji vertė nustatant plaučių vėžio stadiją ir prognozuojant chemoterapijos efektyvumą." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2006. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20060120_124135-72418.
Full textTekorius, Andrius. "Valstybių prevencinė savigyna: teisiniai ir politiniai aspektai." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20101125_190828-24480.
Full textThe paper attempts to prove that the United Nations Security Council (UNSC) due to the absence of mutual agreement between its permanent members and frequent usage of veto right, sanctions the usage of preventive self-defence notably rarely. The problem then occurs: because of the ineffectiveness of the UNSC in sanctioning the usage of preventive self-defence, the states implement the preventive self-defence by themselves, in this way violating one of the main international law principles – refraining from threatening or using force principle. This situation encouraged some big states (permanent members of the UNSC) to foresee the possibility to use unilateral preventive self-defence in their military doctrines in this way ignoring international law. The current situation requires dealing with this issue either legalizing the usage of preventive self-defence or searching for the ways how to force the UNSC to implement its primary task – to maintain international peace and security. The goal of the paper – to reveal the issues of the states’ usage of preventive self-defence emphasizing the Middle East region. The following objectives have been formulated for achieving the goal: 1. To discuss the concept of refraining from threatening or using force principle, the consolidation of this principle in contemporary law as well as analysis of exceptions. 2. To analyze the types of states’ self-defence and find Lithuanian equivalents to English terms of states’ self-defence types:... [to full text]
Burokas, Mantas. "Branduolinės energetikos objektų statybos teisinio reguliavimo ypatumai." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20090122_144141-79907.
Full textNuclear Facility – Legal Regulation of Construction – Nuclear Safety Principles – Special Regime of Legal Regulation Legal regulation of the construction of nuclear facilities is unique of its nature, varying from the construction of the other buildings in enhanced attention for the implementation of nuclear safety measures. The legal regulation of the construction of such an facility becomes more actual in the context of the construction of new nuclear facilities which, in nowadays, becomes more active despite the fact that Lithuania belongs to the group of countries enrolled in nuclear energy, having the goal to increase the capacity of nuclear energy, the present legal regulation of the construction of nuclear facilities is not arranged enough for the consistent and continuous implementation of the construction of new nuclear facilities. The peculiarities and differences of the legal regulation of the new nuclear facilities, in comparison of the legal regulation of other constructions, are obvious in the overall process of construction legal regulation. When Lithuania became a member of the international community, international legal acts became a part of national legal regulation. The construction process of nuclear facilities is closely related to other spheres of legal regulation, especially with territory planning, land law, regulation of the ownership right and others, however the most important feature of legal regulation is the implementation of nuclear safety... [to full text]
Rudzevičius, Ramūnas. "Eglynų branduolio puvinio tyrimai." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2005. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2005~D_20050607_150939-81019.
Full textSavčukas, Tomas. "RELIATYVISTINIŲ PATAISŲ ĮTAKA BRANDUOLIŲ STABILUMUI." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100903_093239-88470.
Full textEarth's daily attempt to find more items, but the searches are very costly, so an increasing number of researchers designed models to calculate the massive nuclear stability. The work consists of: • Introduction • Reviewing the literature • Test work • Findings After work, it was concluded that the relativistic corrections of great importance to the stability of massive nuclei.
Čėplaitė, Šarūnė. "Daugiafunkcinė architektūrinė struktūra - Marijampolės branduolio pamatas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140703_150002-83871.
Full textA town is a territory, divided into functional spaces occupied by living, industry, and service, old and new parts of town. Specific, individual space surrounding each person must be taken into account when planning a city as well as the space itself. The city is home for the local citizenry, and the center of city is the space which can be compared to the sitting-room of the home where various celebrations are held and guests invited. The core of a town is the main topic of the master thesis. The core was built up during a long period of time, has its own development history (therefore buildings associated with different periods of architecture can be found here) and is located in the oldest part of the town. The town square which is the beginning of the development of the city is considered to be the core of the town. Marijampolė is a town of complex past nevertheless it is one of the youngest and currently developing compared to other similar towns in Lithuania. It has its own development history, a core with its advantages and disadvantages, and the core is formed during the years. We are going to get acquainted with the trends of formation of town cores and find out the reasons making influence to the development of towns. The first part of the master thesis is dedicated for the analysis of the term 'core of the town'. Also, the influence a core is making to the town is depicted as well as history of centers of towns, design criteria beginning from the antiquity and... [to full text]
Čėplaitė, Šarūnė. "Daugiafunkcė architektūrinė struktūra - Marijampolės miesto branduoli pamatas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2014~D_20140703_145949-18517.
Full textExplanatory notes.
Venckutė, Milda. "Žinių ekonomikos branduolių kūrimo Lietuvoje problemos ir perspektyvos." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2007. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2007~D_20070108_144728-42271.
Full textKnowledge economy and knowledge economy clusters (high-tech clusters) is one of leading development priority in all countries round the world today. Knowledge Economy clusters implementation in Lithuania allows not only use all possibilities of new Information and Communication and other technologies, but also motivate theirs development and make Lithuania more competitive in the global market. Knowledge Economy cluster is supporting research and development in Lithuania and concurrently promote the Economy growth of the EU. In the work is viewed and analyzed Knowledge Economy clusters establishment problems and perspectives in Lithuania, estimated the theoretical Knowledge Economy cluster dimensions and Knowledge Economy clusters implementation in EU, Finland and Germany Federation. In the first tile of work “Cluster and Knowledge Economy conceptions”, is described theoretical conception and origin of Cluster and Knowledge Economy and systematize practice of implementation of Knowledge Economy clusters and possible effect of Knowledge Economy clusters to economy, which take action in countries round the world. Second tile “Knowledge Economy clusters development and their models in Europe” has two chapters: Knowledge Economy clusters policy in EU” and Knowledge Economy clusters models in several countries”. In this part of the work is measured EU Knowledge Economy clusters policy and examples of Finland and Germany Knowledge Economy clusters models. In the third tile... [to full text]
Fedurtsya, Anton. "Neutronų ir protonų pasiskirstymo atomo branduolio sluoksniuose skaičiavimas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100614_164230-58314.
Full textThe main goal of this thesis is full analysis the algorithm of the distribution of neutrons and protons in the layers of atom nucleus together with a program created by A.Deveikis and the search of places which provide important acceleration for the calculations and configurations of the algorithm. The paper consists of 5 structural parts. In the first part of the paper, optimization methods are described and the results of the research proving the advantages of optimized fragments of the program provided. The second part of the paper consists of a short description of the logic of the algorithm. The third and the fourth part of the paper describe the process of optimization. The last part of the paper proves appropriate operation of the program and foresees the opportunities of the algorithm. As a result, the optimized algorithm of the distribution of neutrons and protons in the layers of atom nuclear was created. The realisation of the algorithm can be used in the calculations of the models of atom nuclear layers and the package of Many Fermion Dynamic code.
Levčenkaitė, Ina. "Kraštinių darinių moreninių gruntų geotechninių savybių ypatumai (branduolinės energetikos objekto statybos aikštelės pavyzdžiu)." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2006~D_20090908_192104-58059.
Full textThis work deals with consolidation and strength features relationship in marginal glacial soils. Soil samples were taken from boreholes core. Boreholes were drilled in Nuclear Power Plant at used nuclear fuel storage facility. The results of test show that the classical laws of soil mechanics of strength dependence in moraine soils are correct in statistical level. Tests prove that strength of moraine loam is multifactor parameter. The strength of that kind of soil depends on quantity of clay particles, consistence and porosity decrease. Porosity decreases when vertical load rises. Therefore classical Coulomb-Navier-Mohr's equation is complicated.
Korenkovaitė, Margarita. "Sergančiųjų sisteminėmis jungiamojo audinio ligomis antikūnų prieš branduolio antigenus paplitimas." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2010~D_20140625_193612-74219.
Full textSUMMARY The prevalence of antibodies agaisnt nuclear antigens in patients in systemic connective tissue diseases The aim of this study was to assess the diagnostic value and to determine the frequency of ENA/ANA in systemic connective tissue diseases (SCTD) and to compare the association of these antibodies with following inflammatory markers ESR, CRP and rheumatoid factor (RF). The analysis involved 50 patiens with seven different diagnosis of connective tissue diseases: mixed connective tissue disease (MITD), systemic lupus eritromatosus (SLE), Sjogren syndrome, systemic sclerodermia, nondifferential connective tissue disease, dermatomiositis/ polimiositis and arthropaties. Sera from each patient was tested for ANA by indirect immunofluoresce (NIF), we assigned, that speckled pattern is appropriate for 60% and homogenous pattern is appropriate for 36% SCTD patients. The result was confirmed by immunoblot method, we estimated, that the prevalence of ENA is as follows anti-Ro-52 (56%), anti-SS-A (44%), anti-SS-B (26%), ds-DNR and nukleosomal antibodies (24%), nRNP/Sm, Scl-70 and hystonic antibodies (14%). Anti-Jo-1 (10%), anti-CENP-B (8%) anti-PCNA (4%). Antibodies to ribosomal and P- Prot antigens (2%). Antibodies to Ku antigens were not detected. Assessing the value of ENA antibodies in differentiation of SCTD, we defined that antibodies against Ro-52 antigens are the only markers detectable in all disease groups, so it could be appraised as a markers of autoimunic systemic... [to full text]
Vaščenkaitė, Galina. "Netikrumo veiksnys tarptautiniuose santykiuose kaip struktūrinės galios didinimo priemonė." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2013. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20131211_185704-78101.
Full textInternational relations can be considered as a set of interactions between the actors of international system. As a result of these interactions some actors gain a reputation of reliable and stable while the others perform as irrational and unpredictable. The main goal of these masters’ theses was to identify when and how should the seemingly “irrational” behavior of the international actor be considered as a rational strategy aiming at some certain objectives. The subject of this research is the interactions between two states in which the structural and comparative power of the one state is lower than the one of the other. Under these circumstances the state which possesses lower power seeks to raise it in relation to the stronger state. The author of this research hypothesizes that the aim of the behavior which seems irrational to the other players of the international system may be the creation of the conditions of uncertainty. The later can be used as a means of rising structural power of the actor who necessitated it. The Author approaches the logic of the theory of rational choice as well as examines the prospects of using the particular models of game theory as a tool of analyzing the abovementioned interactions of two states the structural and comparative power of which is notably unequal. After analyzing the impact of imperfect information as well as the mistrust in the rationality of the partners of the game, the Author comes to conclusion that the abovementioned... [to full text]
Labanauskaitė, Lina. "Elektros srovės, geometrinės optikos, atomo branduolio fizikos mokomieji bandymai ir jų loginė analizė." Bachelor's thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20110103_162236-40276.
Full textBachelor's work „Educational Experiments of Electric Current, Geometrical Optics, Atomic Nuclear Physics and Their Logical Analysis” consists of an introduction, 3 chapters, conclusions and 28 references. Working amount 66 pages. In first and second part gives an overview of pedagogy and psychology analysis of literature source. In third part is described education experiments of physics and their logical reasoning schemes of XII class from: • Electric Current (9 experiments). • Geometrical Optics (8 experiments). • Atomic Nuclear Physics (5 experiments). Reasoning schemes help students to: understand the nature of the demo task, determine cause – effect relationships and dependencies, compare conditions and findings, summarize the results, do conclusions.
Šakenytė, Aurelija. "Hipotetinės ir realios rizikos bei pažeidumai, kylantys iš branduolinio, cheminio ir biologinio terorizmo grėsmių." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2010. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2010~D_20100617_084719-36497.
Full textUnambiguously, the majority of researchers and experts recognize and agree that a terrorist nuclear, biological and chemical weapons use poses a threat to international security. Following a nuclear or large- scale biological or chemical attack would not only trigger enormous humanitarian tragedy, but is likely to push the world on weapons of mass destruction catastrophe. It is therefore necessary to explore what lies beneath these sometimes apocalyptic warnings, and what, indeed, the likelihood that terrorists could use weapons of mass destruction. The object of master thesis- hypothetical and actual risks and vulnerabilities arising from the threats of nuclear, chemical and biological terrorism. The aim of this work is to analyze and assess hypothetical and actual risks and vulnerabilities arising from the threats of nuclear, chemical and biological terrorism. The main tasks of the work: to survey the terrorism of weapons of mass destruction in theoretical perspective of rational choice, a deeper analyzing decision analysis method; to investigate and evaluate the causes which deter terrorists from nuclear, chemical and biological attacks in the implementation; to analyze the reasons that may lead to the nuclear, biological and chemical terrorist attacks in the implementation; to examine and use in practical the influence diagrams of decision making analysis to nuclear, biological or chemical terrorism. The analysis is based on the analytical descriptive... [to full text]
Kajackaitė, Monika. "Vilniaus miesto Neries slėnio urbanistinės plėtros ir apsaugos principai: Žvėryno šiaurinės dalies atvejis." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110627_140355-28090.
Full textThe idea of an article emerged of an observation of architectural – urban complex developement in the right coast of river Neris. This complex is partly materialized 70’s idea of “architectural hills”. Despite of sceptical or even opponential heritage guard’s attitude about this complex, evident that it is successful realization of considerable dimension urban conception. On the other hand, the result contravene primary idea, because it takes considerably bigger area of cities topography, i.e. situated on both sides of Konstitucijos avenue, thuthermore, practical realized changed system of altitude accents, and so on. Distinctly identified visual zones of urban cores in the city raise a question if the mentioned cores organize common compositional system, or if it’s development is based only on local principles of compositional idea. The main aim of this article – suggest methodical development principles of urban structure, considered on territories spatial compositional systems particularity. An article is the result of certain research, in which were evaluated transformations of territories urban structure, compositional importance to visual identity of a city, and structurised perspective strategy of territories urban development. The article consists of: start, the preparatory phase, concept development phase, the findings, design, detailing, and conclusions.
Paulauskas, Vidas. "Finansinių rodiklių analizė, naudojant neuroninius tinklus." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2012~D_20140704_173827-35377.
Full textThe main aim of this work is to analyze a strategy based on the neural networks, Markovian strategies and kernel method, also to create more complex neural network and apply it all for the data of foreign exchange market. Euro and U.S dollar currency pair price was analyzed during the 1 November 2011 - 15 May 2012 period. The closing price of the hourly period was analyzed. The analysis revealed that the best forecasting accuracy shows neural network strategy, followed by the kernel method and end up with Markovian strategy. The results of created more complex neural network are not very good. The analysis of the joint methods revealed that the best accuracy obtained by combining all three methods. But it generates smaller capital than others. It happens, because bets are made only in the third of rounds, and in all other cases there is refusal to make decision. The methods are worth of attention, and which one to choice depends on the person, because bigger capital leads to higher risk.
Koreivaitė, Ieva. "Irano strategijos vertinimas motyvų ir galimybių kontekste." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2014. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2008~D_20140623_181236-94375.
Full textSince Islamic Revolution in 1979 relations between Iran and international community are tensed. Though current situation, which could be described as impasse, has emerged just in the 2002, when the scope of Iran’s nuclear capabilities was first made public by the Iranian opposition group. Still the 2005 could be considered as a date, when Iran‘s and international communities relations gained current structure. In the 2005 new president M.Ahmedi-Nejad has been elected and nuclear works were resumed. Whereupon Iran is demonstrating determinacy to continue its nuclear enrichment and officials who are involved in decision making process are using aggressive rhetoric in account to U.S. an Israel. Though mentioned rhetoric is directed towards U.S. and Israel, other interrelated countries have arrogated these signals to themselves. And they were understood completely different: from assessment that their meaning is coincident with Iran’s peaceful purpose declaration to judgement that they are direct menace towards Western world. Consequently the assessment of Iran’s behaviour and intentions became complicated and that happened for several reasons: first, Iran’s rhetoric is controversial – averment that nuclear programme is peaceful logically don’t merge with declared willingness to destroy opponents. Second, rhetoric and behaviour don’t match - peaceful programme doesn’t go with a concealment of nuclear activities. Third, political assessment still rest upon realism postulates that... [to full text]
Kačinskas, Justas. "Šiaurės Korėjos krizės - jų sprendimo būdai ir įtaka pasaulio saugumui." Master's thesis, Lithuanian Academic Libraries Network (LABT), 2011. http://vddb.laba.lt/obj/LT-eLABa-0001:E.02~2011~D_20110614_111150-03874.
Full textThe object of this major thesis is the Democratic People’s Republic of Korea’s (North Korea) nuclear program and the threat it possesses to international security. The latter are being analyzed within realism theory and Hans J. Morgenthau’s systemic and non-systemic view to the state, R. Cooper’s new and old world order concepts, North Korea’s reactions to international community’s power demonstration in international system. Also, the analysis of international community’s efforts to deal with the emerging situation through multilateral negotiations is being carried out (concentrating the attention to four and six-party talks). In this thesis the statement is being made, that North Korea is viewing international system as rival to her, because it still forms politics according to the old world order concept, that is according to the rules that emerged during Cold War, and this is why the multilateral talks most of the times are meaningless. Lastly, the insights to the future events in Korea peninsula are being proposed. The thesis is composed of three parts. In the first part the analysis of realism theory according to Hans J. Morgenthau is being carried out. In the second part the influence of nuclear North Korea’s program to international security is pointed out. The third part tells why multilateral negotiations were not effective, what is the North Korea’s reaction to the changes in international politics and the future perspectives are being told. This analysis includes... [to full text]
Bielskienė, Kristina. "Analysis of the barley (Hordeum vulgare) tightly bound DNA-protein complexes." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20091202_111955-77123.
Full textŽinoma, kad pastovi nehistoninių polipetidų frakcija yra išgryninama kartu su eukariotine DNR ir sudaro labai tvirtus (galbūt kovalentinius) kompleksus tarp branduolio baltymų ir DNR. Nustatyta, kad Erlicho ascito tvirtuose DNR-baltymų kompleksuose yra baltymas C1D, baltymai, pasižymintys fosfataziniu ir kinaziniu aktyvumais, kai kurie proteazių slopikliai ir kiti, dar neištirti baltymai. Nepaisant intensyvių tyrinėjimų, eukariotinių ląstelių tvirti DNR-baltymų kompleksai vis dar lieka menkai aprašyti ir yra objektas tolimesniems tyrimams. Augalų TBP-DNR kompleksai kol kas buvo tyrinėti labai mažai. Šiame darbe charakterizuojami miežių Hordeum vulgare tvirti DNR-baltymų kompleksai. Mes tyrėme TBP-DNR kompleksus iš miežių skirtingų ūglių organų ir skirtingų vystymosi stadijų ląstelių: lapų, šaknų, koleoptilės. Norint ištirti tokių nukleoproteidų funkcijas, svarbu charakterizuoti individualius komplekso komponentus: polipeptidus ir DNR. Taigi, išskyrėme tvirtai su DNR sąveikaujančius baltymus iš miežių skirtingos diferenciacijos bei skirtingo amžiaus ląstelių: pirminių lapelių, šaknų, koleoptilės ir juos charakterizavome. Taip pat išskyrėme ir charakterizavome DNR fragmentus iš miežių pirminių lapelių bei vandeninės brandos ir pieninės brandos grūdų TBP-DNR kompleksų. Parodėme, kad miežių TBP baltymai yra 15-160 kDa, dauguma baltymų yra rūgštiniai. Kai kurie iš miežių TBP baltymų (10, 25, 38, 40 ir 55 kDa) pasižymi fosfataziniu, galbūt, Ser/Thr aktyvumu. Nustatėme, kad tam... [toliau žr. visą tekstą]
Bielskienė, Kristina. "Miežių (Hordeum vulgare) tvirtų DNR-baltymų kompleksų tyrimas." Doctoral thesis, Lithuanian Academic Libraries Network (LABT), 2009. http://vddb.library.lt/obj/LT-eLABa-0001:E.02~2009~D_20091202_112036-51456.
Full textDespite a great deal of research, the functional significance of tightly bound DNA-protein complexes is not yet clear, therefore these complexes are perfect object for pioneering research. Very little is known about plant TBP-DNA complexes. In this work we investigated barley TBP-DNA complexes from different organs (first leaves, roots and coleoptiles) at different developmental stages. We characterized individual components of tightly bound DNA-proteins complexes: polypeptides (TBP) and DNA. We isolated and characterized TBP proteins from barley first leaves, roots and coleoptiles of different age and differentiation stage. Also we isolated and characterized the DNA fragments from barley first leaves and water ripe and milky ripe grain TBP-DNA complexes. We demonstrated that in different developmental stages of coleoptiles, first leaves and roots TBP-DNA complexes were identified as a group of 15-160 kDa proteins, most of TBPs are acidic. Some of barley TBPs (10, 25, 38, 40 and 55 kDa) exhibit phosphatase, maybe Ser/Thr activity. We have identified also that some of TBPs tyrosines were phosphorylated, this modification depends on organ and developmental stage. Identified barley TBPs were involved in fundamental genetic processes, as well as in chromatin rearrangement and regulation processes. Nuclear matrix proteins, enzymes, transcription factors, serpins, immunophilins, and transposon polypeptides were identified among TBPs. We demonstrated that expression of TBPs depends... [to full text]