Academic literature on the topic 'Bronpol'

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Journal articles on the topic "Bronpol"

1

Butler, Gillian, and Sokratis Stergiadis. "Suitability of bronopol preservative treated milk for fatty acid determination." Journal of Dairy Research 78, no. 2 (2011): 220–25. http://dx.doi.org/10.1017/s0022029911000082.

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This work aimed to test if milk preserved with bronopol can be reliably used for fatty acid determination. Dairy production and milk quality are often monitored regularly to assess performance and contribute to selection indices. With evidence that fat composition can be influenced by selective breeding, there might be an interest in using samples collected in routine testing to evaluate individual cow fatty acid profiles, contributing to breeding indices. However, most recording services use a preservative such as bronopol and there is no published record if this influences subsequent fatty acid analysis. This study used milk from an oil seed supplementation trial, generating a wide range of milk fatty acid profiles, to test if the concentration of 31 individual fatty acids determined by GC were influenced by bronopol. Provided preserved samples are subsequently frozen, milk treated with bronopol can reliably be used to evaluate fatty acid composition in most cases; however bronopol might influence a few long-chain fatty acids present in relatively low concentrations. This is one small step towards simplifying milk compositional analysis but it could ultimately streamline the inclusion of milk fat quality into breeding indices, either with a view to ‘healthier’ milk or potentially reducing methane output and the environmental impact of dairy production.
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2

Weyland, J. W., A. Stern, and J. Rooselaar. "Determination of Bronopol, Bronidox, and Methyldibromo Glutaronitrile in Cosmetics by Liquid Chromatography with Electrochemical Detection." Journal of AOAC INTERNATIONAL 77, no. 5 (1994): 1132–36. http://dx.doi.org/10.1093/jaoac/77.5.1132.

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Abstract A method for the simultaneous determination of methyldibromo glutaronitrile, bronopol, and broni-dox in cosmetics, based on liquid chromatography with electrochemical detection, is described. The method is suitable for both aqueous products and emulsions. The detection limit is better than 0.002% for all analytes. Recoveries from an emulsion, spiked to concentrations of 0.03% of the analytes, were 100.4% for bronopol [relative standard deviation (RSD), 0.43%], 97.1% for bronidox (RSD, 0.83%), and 98.4% for methyldibromo glutaronitrile (RSD, 1.7%). Repeatabilities were calculated from 10 replicate analyses of commercial samples. The repeatability for bronopol from an emulsion containing 0.047% bronopol was 0.0027% (RSD, 1.99%); for bronidox from a lotion containing 0.027% bronidox, 0.0014% (RSD, 1.86%); and for methyldibromo glutaronitrile from an emulsion containing 0.031% methyldibromo glutaronitrile, 0.0019% (RSD, 2.16%). A ruggedness test showed that sample amount influenced the results for all 3 analytes. The results obtained for bronidox also depended on detection parameters and composition of extraction solvent. The method was applied to 138 cosmetic products and performed trouble free during these analyses. Bronopol was found in 14 samples, and bronidox was found in 4 samples, including a baby hair lotion, in which it is prohibited. Methyldibromo glutaronitrile was present in 27 samples, including creams, lotions, and sun protection cosmetics.
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3

Nan, Jing, Shaoran Zhang, Ping Zhan, and Ling Jiang. "Evaluation of Bronopol and Disulfiram as Potential Candidatus Liberibacter asiaticus Inosine 5′-Monophosphate Dehydrogenase Inhibitors by Using Molecular Docking and Enzyme Kinetic." Molecules 25, no. 10 (2020): 2313. http://dx.doi.org/10.3390/molecules25102313.

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Citrus huanglongbing (HLB) is a destructive disease that causes significant damage to many citrus producing areas worldwide. To date, no strategy against this disease has been established. Inosine 5′-monophosphate dehydrogenase (IMPDH) plays crucial roles in the de novo synthesis of guanine nucleotides. This enzyme is used as a potential target to treat bacterial infection. In this study, the crystal structure of a deletion mutant of CLas IMPDHΔ98-201 in the apo form was determined. Eight known bioactive compounds were used as ligands for molecular docking. The results showed that bronopol and disulfiram bound to CLas IMPDHΔ98-201 with high affinity. These compounds were tested for their inhibition against CLas IMPDHΔ98-201 activity. Bronopol and disulfiram showed high inhibition at nanomolar concentrations, and bronopol was found to be the most potent molecule (Ki = 234 nM). The Ki value of disulfiram was 616 nM. These results suggest that bronopol and disulfiram can be considered potential candidate agents for the development of CLas inhibitors.
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4

Lasmar, M. M., M. O. Leite, L. M. Fonseca, et al. "Detection of cheese whey in raw milk preserved with bronopol® through high performance liquid chromatography." Arquivo Brasileiro de Medicina Veterinária e Zootecnia 63, no. 6 (2011): 1553–58. http://dx.doi.org/10.1590/s0102-09352011000600037.

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High performance liquid chromatography was used in order to detect cheese whey in samples of raw milk preserved with Bronopol®. Six samples were collected and divided in 45 aliquots of 40mL. From these, 15 were used as control and stored frozen, 15 were added with Bronopol® and stored at 7ºC, and the other 15 were added with Bronopol® and stored at 30ºC. In all groups, five levels of cheese whey addition (0, 2, 5, 10, and 20%) were tested. The samples were submitted to high performance liquid chromatography on the 2nd, 4th, and 8th days of storage. A completely random design was used, following the factorial scheme (5x3x3) and the results were compared through the non-parametric Kruskal-Wallis test. There was no difference among the treatments (P>0.05), which allows the conclusion that raw milk preserved with Bronopol® may be used for the determination of cheese whey addition in milk through high performance liquid chromatography.
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5

Frosch, P. J., I. R. White, R. J. G. Rycroft, et al. "Contact allergy to Bronopol." Contact Dermatitis 22, no. 1 (1990): 24–26. http://dx.doi.org/10.1111/j.1600-0536.1990.tb01500.x.

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6

Cassoli, Laerte Dagher, Paulo Fernando Machado, and Arlei Coldebella. "Métodos de conservação de amostras de leite para determinação da contagem bacteriana total por citometria de fluxo." Revista Brasileira de Zootecnia 39, no. 2 (2010): 434–39. http://dx.doi.org/10.1590/s1516-35982010000200029.

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Avaliou-se o efeito da temperatura de armazenamento e da idade da amostra sobre a contagem bacteriana total de amostras de leite conservadas com azidiol para verificar a possibilidade de utilização de uma única amostra nas análises previstas na Instrução Normativa 51 (IN-51). Utilizaram-se 320 alíquotas de leite coletado de um tanque de expansão para avaliação de três temperaturas de armazenamento - a 0 ºC (congelada), 7 ºC (refrigerada) e 24 ºC (à temperatura ambiente) -, três tipos de conservação (bronopol, azidiol e sem conservante) e quatro idades da amostra (1, 3, 5 e 7 dias). Para contagem bacteriana total, foram consideradas controle amostras refrigeradas, com azidiol e amostras de um dia e, para composição e contagem de células somáticas (CCS), os controles foram amostras refrigeradas, com bronopol e de um dia. Nas amostras conservadas com bronopol, a contagem bacteriana total foi menor que na controle, independentemente da idade e da temperatura. O mesmo foi observado nas amostras com azidiol e congeladas. Amostras com azidiol apresentaram aumento da contagem bacteriana total, independentemente da idade, enquanto, naquelas que não receberam azidiol, a contagem bacteriana total foi superior à das amostras refrigeradas, conservadas com azidiol e de um dia. As amostras conservadas com azidiol e refrigeradas tiveram aumento linear na contagem bacteriana total de 0,0058 log por dia, o que pode ser considerado sem importância prática. Amostras conservadas com azidiol e refrigeradas apresentaram CCS menor, em todas as idades, em relação a amostras refrigeradas e conservadas com bronopol. Os teores de gordura e lactose reduziram após o quinto dia. Foi necessária a coleta de duas amostras: uma destinada à determinação de CCS e da composição contendo bronopol e outra para contagem bacteriana total contendo azidiol. Amostras para contagem bacteriana total podem ser analisadas em até sete dias, se mantidas a 7 ºC e adicionadas de azidiol.
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7

Ferreira, KdeS, L. Tachibana, DRdaS Colombo, SCOda Paixão, CM Ferreira, and C. Badaró-Pedroso. "Toxic effects in Aphanomyces brasiliensis and zebrafish embryos caused by oomyceticides." Diseases of Aquatic Organisms 144 (April 8, 2021): 75–87. http://dx.doi.org/10.3354/dao03575.

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In fish farming, high losses occur during egg incubation and larviculture due to diseases caused by oomycetes. This study aimed to identify the oomycete species that occurs in zebrafish Danio rerio eggs and to evaluate the oomyceticidal effect of copper sulfate, bronopol and methylene blue on the mycelial growth of this organism, as well as to determine the lethal and sublethal toxicity of these compounds in embryos of D. rerio. The isolates were cultivated in yeast-starch medium to determine the concentration necessary to inhibit mycelial growth by 50% (IC50) and 100% (minimum oomyceticidal concentration) after a 96 h exposure to these compounds. In addition, tests with D. rerio eggs were conducted to determine the lethal concentrations for 50% of the organisms (96h-LC50), and the concentrations that inhibited 17% of the eggs hatching (96h-IC17) after 96 h. The organism responsible for the mortality of D. rerio eggs was classified by classical and molecular methods as Aphanomyces brasiliensis, representing the first report of this pathogen in zebrafish eggs. IC50 values could be determined for both bronopol and copper sulfate, whereas methylene blue had low effectiveness against the oomycete. Copper showed high toxicity to D. rerio eggs at low concentrations, while methylene blue and bronopol toxicity was low and similar to each other. The use of bronopol at a concentration of 4.8 mg l-1 for the treatment of zebrafish eggs allows controlling the pathology without causing deleterious effects to the treated organisms.
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8

Camarasa, José G. "Contact dermatitis due to Bronopol." Contact Dermatitis 14, no. 3 (1986): 191. http://dx.doi.org/10.1111/j.1600-0536.1986.tb01214.x.

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9

Arcuri, Edna Froeder, Priscilla Diniz Lima da Silva, José Renaldi Feitosa Brito, Marcio Roberto Silva, and Guilherme Nunes Souza. "Emprego do Somacount 300, calibrado com leite de vaca, na contagem de células somáticas no leite de cabra." Ciência Rural 34, no. 5 (2004): 1497–500. http://dx.doi.org/10.1590/s0103-84782004000500026.

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Neste estudo, foi comparada a contagem de células somáticas (CCS) de amostras pareadas de leite de 86 cabras pelo método eletrônico (Somacount 300) calibrado com padrão de vaca, com a técnica padrão de contagem microscópica direta utilizando corante pyronina Y - verde de metila. As cabras eram das raças Saanen e Toggenburg de um criatório localizado na Zona da Mata de Minas Gerais. Em complementação, foi avaliado o efeito do conservante Bronopol® no leite de cabras sobre a CCS. Para as 86 amostras de leite sem conservante, a média das leituras de CCS determinadas com o microscópio foi menor e diferiu (p <FONT FACE=Symbol>£</FONT> 0,05) da média de CCS lidas pelo Somacount 300 padronizado com leite de vaca. Porém, a média de leituras de CCS das 86 amostras com Bronopol® lidas no microscópio não apresentou diferença significativa (p>0,05) em relação à obtida com o Somacount 300; e a curva de estimação de CCS no microscópio em função do Somacount foi significativa em nível de 95% com alta correlação, demonstrando a possibilidade de se utilizar o Somacount 300 padronizado com leite de vaca para leituras de CCS de leite de cabras conservado com Bronopol® dentro dos limites de CCS analisados neste estudo.
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10

Scott, Kitty. "Introduction to AA Bronson / Présentation de AA Bronson." National Gallery of Canada Review 12 (September 1, 2021): iv—8. http://dx.doi.org/10.3138/ngcr.12-001.

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