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Journal articles on the topic 'Broth media'

Author: Grafiati

Published: 4 June 2025

Last updated: 1 August 2025

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1

SILK, TODD M., TATIANA M. T. ROTH, and C. W. DONNELLY. "Comparison of Growth Kinetics for Healthy and Heat-Injured Listeria monocytogenes in Eight Enrichment Broths." Journal of Food Protection 65, no. 8 (2002): 1333–37. http://dx.doi.org/10.4315/0362-028x-65.8.1333.

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Detection of Listeria in food products is often limited by performance of enrichment media used to support growth of Listeria to detectable levels. In this study, growth curves were generated using healthy and heat-injured Listeria monocytogenes strain F5069 in three nonselective and five selective enrichment broths. Nonselective enrichment media included the current Food and Drug Administration Bacteriological Analytical Manual Listeria enrichment broth base (BAM), Listeria repair broth (LRB), and Trypticase soy broth. Selective enrichment media included BAM with selective agents and LRB with

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2

Hammack, Thomas S., Mildred L. Johnson, Andrew P. Jacobson, and Wallace H. Andrews. "Effect of Sample Preparation and Preenrichment Media on the Recovery of Salmonella from Cantaloupes, Mangoes, and Tomatoes." Journal of AOAC INTERNATIONAL 89, no. 1 (2006): 180–84. http://dx.doi.org/10.1093/jaoac/89.1.180.

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Abstract Studies were conducted to determine the relative effectiveness of buffered peptone water (BPW), lactose (LAC) broth, and Universal Preenrichment (UP) broth for the recovery of Salmonella organisms from fruit rinses, whole fruit, and comminuted fruit. In the first phase, the relative effectiveness of the rinse and soak methods for the recovery of Salmonella from surface-contaminated mangoes and tomatoes was examined. Fruits were spot inoculated with single Salmonella serovars and held for 4 days at 26C before analysis was initiated. The contaminated fruit was rinsed in portions of BPW,

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3

Sousa, José Mário, Ana Barbosa, Daniela Araújo, et al. "Evaluation of Simultaneous Growth of Escherichia coli O157:H7, Salmonella spp., and Listeria monocytogenes in Ground Beef Samples in Different Growth Media." Foods 13, no. 13 (2024): 2095. http://dx.doi.org/10.3390/foods13132095.

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Several multiplex approaches for the simultaneous detection of pathogens in food have been developed in recent years, but the use of a single enrichment medium remains a problem. In this study, six enrichment broths (five non-selective media, tryptic soy broth (TSB), brain heart infusion broth (BHI), buffered peptone water (BPW), universal pre-enrichment broth (UPB), no. 17 broth, and a selective, Salmonella Escherichia Listeria broth (SEL)), were studied for the simultaneous detection of E. coli O157:H7, Salmonella spp., and L. monocytogenes, to validate the suitable enrichment broth to be us

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4

Iversen, Carol, and Stephen J. Forsythe. "Comparison of Media for the Isolation of Enterobacter sakazakii." Applied and Environmental Microbiology 73, no. 1 (2006): 48–52. http://dx.doi.org/10.1128/aem.01562-06.

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ABSTRACT Enterobacter sakazakii is associated with neonatal infections and is occasionally present at low levels (<1 CFU/g) in powdered infant formula milk (IFM). It has been previously reported that some E. sakazakii strains do not grow in standard media for Enterobacteriaceae and coliform bacteria; therefore, a reliable method is needed for recovery of the organism. Three E. sakazakii enrichment broths—Enterobacteriaceae enrichment broth (EE), E. sakazakii selective broth (ESSB), and modified lauryl sulfate broth (mLST)—were compared with a novel broth designed for maximum recovery of E.

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5

SMITH, JAMES L., and BRIAN J. DELL. "Capability of Selective Media to Detect Heat-Injured Shigella flexneri." Journal of Food Protection 53, no. 2 (1990): 141–44. http://dx.doi.org/10.4315/0362-028x-53.2.141.

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Heat-injured cells of Shigella flexneri 5348 were detected by using a two-media plating system: tryptic soy broth containing 2% agar plus 1% sodium pyruvate (TSBA + P) on which both injured and non-injured cells produce colonies and semisynthetic agar medium plus 0.15% bile salts #3 (SSBA) on which only non-injured cells grew. Subtracting the count on SSBA from that on TSBA + P gave an estimation of injury. Heating washed cells of S. flexneri in phosphate buffer gave about 3 log units of injury after 40 min at 52°C. A number of broths and agars specific for shigellae and gram-negative bacteria

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6

Stephenson, Patricia, Felicia B. Satchell, Geraldine Allen, and Wallace H. Andrews. "Recovery of Salmonella from Shell Eggs." Journal of AOAC INTERNATIONAL 74, no. 5 (1991): 821–26. http://dx.doi.org/10.1093/jaoac/74.5.821.

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Abstract A preenrlchment procedure and a direct selective enrichment procedure were compared for recovery of Salmonella artificially inoculated into liquid whole egg, egg yolk, and egg albumen. For liquid whole egg and egg yolk, the 2 procedures were comparable. With egg albumen, however, preenrlchment In lactose broth gave significantly higher recoveries than did direct selective enrichment in either selenite cystine or tetrathionate broths. The lactose preenrlchment procedure was used to determine the survival of S. enterltldla in egg yolk and egg albumen over a period of 7 days. As shown by

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7

Swaminathan, B., Peggy S. Hayes, Vincent A. Przybyszewski, and Brian D. Plikaytis. "Evaluation of Enrichment and Plating Media for Isolating Listeria monocytogenes." Journal of AOAC INTERNATIONAL 71, no. 3 (1988): 664–68. http://dx.doi.org/10.1093/jaoac/71.3.664.

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Abstract We compared selective enrichment broths used by the U.S. Food and Drug Administration (FDA) and the U.S. Department of Agriculture (USDA), Food Safety and Inspection Service, for their efficiency in the quantitative recovery of Listeria monocytogenes from a naturally contaminated Brie cheese that was obtained as part of an epidemic investigation. Quantitative recovery of Listeria in FDA broth (&gt;2.4 x 10s colony forming units/mL) was significantly better than recovery in USDA broth (9.3 x 103 colony forming units/mL). When USDA broth was supplemented with i&gt;glucose and Ph

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8

ZHENG, QIANWANG, CAROLINE BUSTANDI, YISHAN YANG, KEITH R. SCHNEIDER, and HYUN-GYUN YUK. "Comparison of Enrichment Broths for the Recovery of Healthy and Heat-Injured Salmonella Typhimurium on Raw Duck Wings." Journal of Food Protection 76, no. 11 (2013): 1963–68. http://dx.doi.org/10.4315/0362-028x.jfp-13-041.

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This study was performed to optimize Salmonella Typhimurium recovery from raw duck wings with five nonselective broths (buffered peptone water, tryptic soy broth, lactose broth, universal preenrichment broth, nutrient broth) and four selective broths (selenite broth, BAX System MP media [MP], Salmonella AD media [AD], ONE broth-Salmonella [OB]). Healthy or heat-injured (50 and 85% injury) cells were inoculated at a level of 102, 101, or 100 CFU/25 g on raw duck wings. Growth was modeled using DMfit with four growth parameters: lag-phase duration, maximum growth rate, doubling time, and maximum

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9

ABEYTA, CARLOS, STEPHEN D. WEAGANT, CHARLES A. KAYSNER, et al. "Aeromonas hydrophila in Shellfish Growing Waters: Incidence and Media Evaluation." Journal of Food Protection 52, no. 1 (1989): 7–12. http://dx.doi.org/10.4315/0362-028x-52.1.7.

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Levels of Aeromonas hydrophila determined for the shellfish growing area of Grays Harbor, Washington, ranged from 3 to 4600/100 g in oysters and from 3 to 2400/100 ml in water. Of isolates tested, 80% produced a hemolysin, a trait reported to correlate with enterotoxin production and pathogenicity. Two enrichment broths, Tryptic Soy Broth with ampicillin (TSBA) and Modified Rimler Shotts Broth (MRSB) were compared in combination with three solid agar media: Rimler Shotts (RS), Peptone Beef Extract Glycogen (PBG), and MacConkey's (MCA) agars. TSBA was far superior to MRSB in isolating this spec

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10

Hammack, Thomas S., Andrew P. Jacobson, and Wallace H. Andrews. "The Effect of Preenrichment and Selective Enrichment Media on Recovery of Salmonella Typhi from the Tropical Fruit Mamey." Journal of AOAC INTERNATIONAL 91, no. 1 (2008): 83–91. http://dx.doi.org/10.1093/jaoac/91.1.83.

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Abstract The Bacteriological Analytical Manual (BAM) Salmonella culture method did not detect Salmonella Typhi in mamey, the tropical fruit that was implicated in a 1999 typhoid outbreak. The relative effectiveness of BAM's nonselective preenrichment and selective media for the recovery of S. Typhi from mamey was examined to determine if the BAM's preenrichment/selective enrichment strategy was the cause of the method's failure with this food. The preenrichment media were lactose broth, buffered peptone water (BPW), and universal preenrichment (UP) broth. The selective enrichment media were se

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11

Wahyudi, Didik, and Endang Sutariningsih Soetarto. "Pembentukan Biofilm Pseudomonas aeruginosa pada Beberapa Media Cair." Jurnal Farmasi (Journal of Pharmacy) 10, no. 2 (2021): 35–40. http://dx.doi.org/10.37013/jf.v10i2.142.

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Pseudomonas aeruginosa merupakan bakteri Gram negatif berbentuk batang bersifat patogen oportunistik yang menjadi penyebab utama infeksi nosokomial dan mampu membentuk biofilm pada media pertumbuhan, biofilm sering mengakibatkan pengobatan penyakit infeksi menjadi lebih sulit. Media pertumbuhan bakteri ada beberapa jenis, komposisi dan merek. Tujuan penelitian ini adalah untuk mengetahui kemampuan P. aeruginosa dalam membentuk biofilm pada beberapa media biakan cair. P. aeruginosa diisolasi dari sampel klinis dari rumah sakit, media cair yang digunakan adalah nutrien broth, laktosa broth, brai

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12

HARRIS, L. J., and M. E. STILES. "Reliability of Escherichia coli Counts for Vacuum-Packaged Ground Beef." Journal of Food Protection 55, no. 4 (1992): 266–70. http://dx.doi.org/10.4315/0362-028x-55.4.266.

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Test strains of Escherichia coli were inoculated into fresh ground beef that had been irradiated or carefully excised and aseptically ground. Samples were vacuum-packaged and stored at 4°C. Plate counts on selective media incubated at 35 or 45°C were highly consistent during the 7- to 20-d storage periods. The standard most probable number (MPN) technique (lauryl tryptose broth at 35°C, followed by EC broth at 45°C) was also reliable. In contrast, direct inoculation into broths incubated at 45°C gave unreliable and highly variable results. The cause of the variability of the MPN counts at 45°C

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13

AL-HOLY, M. A., J. H. SHIN, T. M. OSAILI, and B. A. RASCO. "Evaluation of a New Enrichment Broth for Detection of Cronobacter spp. in Powdered Infant Formula." Journal of Food Protection 74, no. 3 (2011): 387–93. http://dx.doi.org/10.4315/0362-028x.jfp-10-239.

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The aim of this study was to investigate the potential of using Al-Holy–Rasco (AR) medium, a novel broth for detection and isolation of Cronobacter spp. in infant formula milk (IFM). The new medium's composition is generic brain heart infusion broth with the addition of 1% NaCl, 15% sucrose, and 0.80 g/liter sodium deoxycholate as selective ingredients. AR broth outperformed Enterobacteriaceae enrichment broth (EE), Enterobacter sakazakii enrichment broth (ESE), modified lauryl sulfate broth, and milk as enrichment media to stimulate the growth of a cocktail of 10 strains of Cronobacter. Addit

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14

Pawlikowska, Ewelina, Jaroslaw Domanski, Piotr Dziugan, et al. "Comparison of Three Deoxidation Agents for Ozonated Broths Used in Anaerobic Biotechnological Processes." Processes 7, no. 2 (2019): 65. http://dx.doi.org/10.3390/pr7020065.

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Anaerobic fermentation of organic compounds is used in many biotechnological processes and has been the subject of much research. A variety of process conditions and different growth media can be used to obtain microbial metabolites. The media must be free from contamination before fermentation. Sterilization is most often achieved by applying heat or other treatments, such as ozonation. Sterilization of liquid media using ozone can be very beneficial, but this method introduces high concentrations of residual oxygen, which inhibit anaerobic processes. Deoxidation is therefore necessary to rem

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15

BAILEY, J. S., D. L. FLETCHER, and N. A. COX. "Efficacy of Enrichment Media for Recovery of Heat-Injured Listeria monocytogenes." Journal of Food Protection 53, no. 6 (1990): 473–77. http://dx.doi.org/10.4315/0362-028x-53.6.473.

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The efficacy of University of Vermont (UVM) and Listeria enrichment broth (LEB) enrichment broths for the recovery of heat-injured Listeria monocytogenes was determined in pure culture and in the presence of competitive microflora from chicken and Brie cheese. Both UVM and LEB were found to be effective for recovery of viable L. monocytogenes. Only UVM consistently allowed recovery of heat-injured L. monocytogenes. The different concentrations of NaCl used in these media did not account for these differences. The pH of LEB and UVM broth containing mixed microflora from chicken and Brie cheese

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16

Kondratiuk, T., T. Akulenko, E. Torgalo, T. Beregova, and L. Ostapchenko. "Dependence of biomass accumulation by melanin producer Psedonadsoniella BRUNNEA (Meripilaceae, Agaricomycotina) of the cultural medium content." Bulletin of Taras Shevchenko National University of Kyiv. Series: Biology 77, no. 1 (2019): 83–86. http://dx.doi.org/10.17721/1728_2748.2019.77.83-86.

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Dependence of biomass accumulation by melanin producer Pseudonadsoniella brunnea (Merepilaceae, Agaricomycotina) of the cultural medium content was aim of this study. The following 14 different types of cultural media were used: standard liquid nutrient media (Malt extract broth – МЕВ), Sabouraud Broth, potato-dextrose Broth, as well as the original (modified) nutritional media, based on Malt extract Broth, barley malt extract and Nutrient Broth. Various components were used for the modified media, in particular 0.9 % NaCl, monosaccharides (D-glucose, fructose, D (+) – xylose), disaccharides (

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17

Ruchi, Agrawal, Shah Mitesh, Singh Puja, and Ranjan K.P. "Comparison of Efficacy of Biphasic BHI Media, Tryptic Soy Broth and Glucose Broth in Isolating Bacteria through Blood Culture of Suspected Septicemia Patients." International Journal of Pharmaceutical and Clinical Research 15, no. 9 (2023): 1414–19. https://doi.org/10.5281/zenodo.11389933.

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<strong>Introduction: </strong>Blood stream infection (BSI), bacteremia and thereby septicaemia remains one of the most important causes of morbidity and mortality throughout the world. The diagnosis of these infections can be confirmed by blood culture which is the gold standard for the diagnosis. No single blood culture medium or system is capable of detecting all the micro-organisms. Present study compares the efficacy of three culture media for isolation of bacteria through blood culture of suspected septicemia patients. <strong>Material & Methods: </strong>Study was con

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18

MOORE, JOHN E., and ROBERT H. MADDEN. "Impediometric Detection of Campylobacter coli." Journal of Food Protection 65, no. 10 (2002): 1660–62. http://dx.doi.org/10.4315/0362-028x-65.10.1660.

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The rapid automated bacterial impedance technique (RABIT) was examined as a method for the detection of two wild-type isolates of Campylobacter coli in broth media. Both isolates failed to produce a change in impedance that was sufficient for detection in any combination of six nonselective basal broth media, including Mueller-Hinton broth, nutrient broth no. 2, brain heart infusion broth supplemented with yeast extract (0.5% [wt/vol]), brucella broth, Campy broth supplemented with yeast extract (0.5% [wt/vol]), and Whitley impedance broth, at 37 and 42°C. Although the strains did proliferate

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19

LIEWEN, MICHAEL B., and ELMER H. MARTH. "Growth of Sorbate-Resistant and -Sensitive Strains of Penicillium roqueforti in the Presence of Sorbate." Journal of Food Protection 48, no. 6 (1985): 525–29. http://dx.doi.org/10.4315/0362-028x-48.6.525.

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Growth of two strains of Penicillium roqueforti in different media foritified with sorbate was observed. The sorbic acid-sensitive strain grew in YES and mycological broths with sorbic acid concentrations up to 500 ppm, and in YM broth with sorbic acid concentrations up to 1000 ppm. The sorbic acid-resistant strain grew in YES and mycological broths with sorbic acid concentrations up to 6000 ppm, and in YM broth with sorbic acid concentrations up to 9000 ppm. Supplementing mycological broth with various ingredients affected growth of the resistant strain. Addition of 1% casein or 0.3% yeast, p

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PAPADOPOULOU, C., and E. XYLOURI. "An Assessment of Rappaport-Vassiliadis Medium Based on Growth Kinetics." Journal of Food Protection 52, no. 4 (1989): 252–53. http://dx.doi.org/10.4315/0362-028x-52.4.252.

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The growth of eleven Salmonella serotypes cultured in modified Rappaport-Vassiliadis medium was compared with the growth in selenite broth and in nutrient broth. The growth of other enterobacteria in the three media was also studied. The mean generation times of all the serotypes were calculated by fitting the experimental data to the exponential growth law. The results showed that Rappaport-Vassiliadis medium sustained the growth of nine Salmonella serotypes and proved more successful in limiting the growth of other enterobacteria than did the selenite or nutrient broths.

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Fashola, M. O., O. S. Anagun, K. O. Adebiyi, et al. "Effect of growth media on heavy metal tolerant bacteria isolated from Owode-Onirin iron scrap market in Nigeria." Ife Journal of Science 26, no. 3 (2025): 757–70. https://doi.org/10.4314/ijs.v26i3.16.

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Heavy metals often form complexes with the organic components of most growth media resulting in decrease metal availability to bacteria cells. Hence, this study assessed different growth media used in studying heavy metal tolerance in bacteria vis elucidating the impact of heavy metals on the biochemical traits of heavy-metal tolerant bacteria. Standard cultural techniques were used to recover heavy metals (cadmium, lead and nickel) tolerant bacteria from Owode-Onirin metal-laden soil. The isolates were presumptively identified using their biochemical characteristics and further authenticated

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Moura, Paula Francislaine, Celso Garcia Auer, Katlin Suellen Rech, et al. "Biotechnology of biomass production in vitro of fungi isolated from Pinus." Research, Society and Development 9, no. 10 (2020): e7809109080. http://dx.doi.org/10.33448/rsd-v9i10.9080.

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Fungi are organisms capable of synthesizing metabolites of industrial interest and the standardization of biomass production for the extraction of these compounds has biotechnological applications. The objective of this work was to optimize the in vitro cultivation process for fungi isolated from Pinus sp., standardizing the best conditions for the production of biomass, contributing to its large scale production. Therefore, the conditions of in vitro cultivation of the fungi Botrytis cinerea, Rhizoctonia sp. and Suillus sp., were evaluated based on the maximum production of dry biomass (PBS),

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HAMMACK, THOMAS S., IRIS E. VALENTIN-BON, ANDREW P. JACOBSON, and WALLACE H. ANDREWS. "Relative Effectiveness of the Bacteriological Analytical Manual Method for the Recovery of Salmonella from Whole Cantaloupes and Cantaloupe Rinses with Selected Preenrichment Media and Rapid Methods†." Journal of Food Protection 67, no. 5 (2004): 870–77. http://dx.doi.org/10.4315/0362-028x-67.5.870.

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Soak and rinse methods were compared for the recovery of Salmonella from whole cantaloupes. Cantaloupes were surface inoculated with Salmonella cell suspensions and stored for 4 days at 2 to 6° C. Cantaloupes were placed in sterile plastic bags with a nonselective preenrichment broth at a 1:1.5 cantaloupe weight-to-brothvolume ratio. The cantaloupe broths were shaken for 5 min at 100 rpm after which 25-ml aliquots (rinse) were removed from the bags. The 25-ml rinses were preenriched in 225-ml portions of the same uninoculated broth type at 35° C for 24 h (rinse method). The remaining cantaloup

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Hsu, Wei-Yea, Cheng-i. Wei, and Mark L. Tamplin. "Enhanced Broth Media for Selective Growth ofVibrio vulnificus." Applied and Environmental Microbiology 64, no. 7 (1998): 2701–4. http://dx.doi.org/10.1128/aem.64.7.2701-2704.1998.

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ABSTRACT Rapid detection of Vibrio vulnificus can be enhanced by optimizing the components of enrichment broth. PNC (5% peptone, 1% NaCl, and 0.08% cellobiose [pH 8.0]) enhanced the growth of V. vulnificus compared to alkaline peptone broth. PNCC (PNC with 1.0 to 4.1 U of colistin methanesulfonate per ml) increased the growth of low levels of V. vulnificus while suppressing non-target bacteria.

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Vardhan, Shubhranshu, Aruna Parihar, KPS Kushwaha, and Sandeep Arora. "Selection of the Best Performing Media for the Growth and Development of Cordyceps militaris Mycelia." Journal of Advances in Biology & Biotechnology 27, no. 12 (2024): 681–94. https://doi.org/10.9734/jabb/2024/v27i121816.

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Since ages, the genus Cordyceps has been used as a medicinal fungus for augmenting endurance, longevity and vitality. This fungus commands a high commercial value due to scarcity in its availability and high global market demand. In the present investigations different defined media viz, agar, optimised nutrient broth, chitosan + malt extract agar, chitosan + agar + optimised nutrient broth, Kenknights and Munnaiers, malt extract agar, malt extract agar + optimised nutrient broth, oat meal agar, rice extract agar, rice extract agar + optimised nutrient broth and rose bengal agar have been used

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BIASIZZO, MAJDA, STANKA VADNJAL, URSKA HENIGMAN, MANJA KRIZMAN, ANDREJ KIRBIS, and URSKA JAMNIKAR-CIGLENECKI. "Development and Validation of a New Protocol for Detecting and Recovering Clostridium difficile from Meat Samples." Journal of Food Protection 81, no. 4 (2018): 561–68. http://dx.doi.org/10.4315/0362-028x.jfp-17-354.

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ABSTRACT There is no recommended protocol for detecting and isolating Clostridium difficile present in food samples. Here, we have evaluated the recovery of C. difficile in meat samples after incubating them in various enrichment broths. The media were as follows: cycloserine-cefoxitin fructose broth supplemented with taurocholic acid, d-cycloserine, cefoxitin, and lysozyme; cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme; and cycloserine-cefoxitin fructose broth supplemented with taurocholic acid, C. difficile moxalactam norfloxacin selective supplement, and lysozyme. Samp

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Kumar, Rakesh, Poothuvallil K. Surendran, and Nirmala Thampuran. "Evaluation of Culture Media for Selective Enrichment and Isolation of Salmonella in Seafood." Journal of AOAC INTERNATIONAL 93, no. 5 (2010): 1468–71. http://dx.doi.org/10.1093/jaoac/93.5.1468.

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Abstract Seafood, including fish, shrimp, clam, crab, mussel, oyster, lobster, squid, octopus, and cuttlefish samples, was used to compare the recovery of Salmonella serovars by different selective enrichment and isolation media. The samples were selectively enriched in Rappaport-Vassiliadis (RV) broth and tetrathionate broth (TT), followed by selective isolation on Hektoen enteric (HE) agar, xylose lysine desoxycholate (XLD) agar, bismuth sulfite (BS) agar, and Brilliant Green (BG) agar media. Of 443 seafood samples analyzed, 108 were found to be contaminated with Salmonella. The role of sele

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Hulankova, Radka. "The Influence of Liquid Medium Choice in Determination of Minimum Inhibitory Concentration of Essential Oils against Pathogenic Bacteria." Antibiotics 11, no. 2 (2022): 150. http://dx.doi.org/10.3390/antibiotics11020150.

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So far there is no internationally accepted, standardized method for MIC determination of natural substances such as essential oils (EOs). The aim of this study was to elucidate how much the MIC values obtained from various studies using different culture media are comparable. The median MICs for cinnamon essential oil (EO) obtained by broth dilution were 517, 465 and 517 µg/mL for Mueller–Hinton Broth (MHB), Tryptone Soya Broth (TSB) and Brain Heart Infusion (BHI), respectively. The MIC values for oregano EO were significantly (p < 0.001) lower in MHB than in highly nutritious media; the m

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Nurjanna, Nurjanna. "POLA PERTUMBUHAN BAKTERI PROBIOTIK PADA MEDIA NUTRIENT BROTH." Buletin Teknik Litkayasa Akuakultur 5, no. 1 (2017): 53. http://dx.doi.org/10.15578/blta.5.1.2006.53-55.

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Scythes, K. D., M. Louie, and A. E. Simor. "Evaluation of nutritive capacities of 10 broth media." Journal of clinical microbiology 34, no. 7 (1996): 1804–7. http://dx.doi.org/10.1128/jcm.34.7.1804-1807.1996.

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PRITCHARD, TODD J., and CATHERINE W. DONNELLY. "Combined Secondary Enrichment of Primary Enrichment Broths Increases Listeria Detection." Journal of Food Protection 62, no. 5 (1999): 532–35. http://dx.doi.org/10.4315/0362-028x-62.5.532.

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The efficacy of combining dual primary enrichment cultures into a single secondary broth was evaluated for detecting Listeria in naturally contaminated meats and environmental samples obtained from dairy processing plants. A total of 336 samples were tested using University of Vermont modified Listeria enrichment broth (UVM) and Listeria repair broth containing selective agents (LRBS) as primary enrichment media. Eighty samples (23.8%) yielded Listeria by at least one method. Neither primary enrichment broth was significantly better (P &gt; 0.05) than the other in identifying Listeria-posi

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TERAMURA, HAJIME, MIHOKO IWASAKI, and HIROKAZU OGIHARA. "Development of a Novel Chromogenic Medium for Improved Campylobacter Detection from Poultry Samples." Journal of Food Protection 78, no. 9 (2015): 1750–55. http://dx.doi.org/10.4315/0362-028x.jfp-15-115.

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The presence of expanded-spectrum β-lactamase (ESBL)–producing Escherichia coli is a common problem in the isolation of Campylobacter from poultry samples using conventional cefoperazone-based selective media. A novel chromogenic medium (CM-HT), based on modified charcoal cefoperazone deoxycholate agar (mCCDA), has been developed as a solution for improved Campylobacter detection from poultry samples. Although the basic components of CM-HT are the same as mCCDA, CM-HT uses both granular charcoal and sodium cefoxitin to enhance viewability and inhibit ESBL-producing bacteria. All tested Campylo

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Wijesinghe, Gayan, Ayomi Dilhari, Buddhika Gayani, Nilwala Kottegoda, Lakshman Samaranayake, and Manjula Weerasekera. "Influence of Laboratory Culture Media on in vitro Growth, Adhesion, and Biofilm Formation of Pseudomonas aeruginosa and Staphylococcus aureus." Medical Principles and Practice 28, no. 1 (2018): 28–35. http://dx.doi.org/10.1159/000494757.

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Objective: Pseudomonas aeruginosa and Staphylococcus aureus dual-species biofilm infections are notoriously difficult to manage. This study aimed at investigating the influence of four different culture media on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa and S. aureus. Materials and Methods: We monitored four different culture media including Nutrient Broth, Brain Heart Infusion (BHI) broth, Luria-Bertani broth, and RPMI 1640 medium on the planktonic growth, adhesion, and biofilm formation of P. aeruginosa (ATCC 27853) and S. aureus (ATCC 25923) using MTT assay and

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HAMMACK, THOMAS S., R. MIGUEL AMAGUAÑA, and WALLACE H. ANDREWS. "An Improved Method for the Recovery of Salmonella Serovars from Orange Juice Using Universal Preenrichment Broth." Journal of Food Protection 64, no. 5 (2001): 659–63. http://dx.doi.org/10.4315/0362-028x-64.5.659.

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The relative effectiveness of three methods for the recovery of Salmonella serovars from orange juice was determined. One method, a modified Bacteriological Analytical Manual (BAM) procedure consisted of preenrichment in lactose broth at 35°C for 24 h, selective enrichment, and selective plating. Another method, a National Centers for Disease Control and Prevention (CDC 1) procedure, consisted of direct enrichment in tetrathionate broth at 35°C for 24 and 48 h, followed by selective plating. The third method (also from CDC and designated CDC 2) consisted of preenrichment in Universal Preenrich

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Awaludin Prihanto, Asep, Kartika D. Aninta, and Soffi Trisnaningrum. "Protease Production from Bacillus sp. Isolated from Gastrointestinal Tract of Catfish (Clarias sp.) with Different Medium." Journal of Aquaculture and Fish Health 10, no. 2 (2021): 186. http://dx.doi.org/10.20473/jafh.v10i2.17606.

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The need for protease in the industrial field has been increasing. Candidates for producing these enzymes can be isolated from the digestive tract of catfish (Clarias sp.). The purpose of this study was to obtain bacterial isolates that produce proteolytic from the gastrointestinal tract of catfish and determine the effect of different production media on the activity of proteolytic. The first step of this study was isolation, screening, and identification of bacteria. The second step was to test the effect of the media Luria Bertani, trypticase soy broth, and skim milk broth on proteolytic ac

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Hidayati, Nurul, Dhika Juliana Sukmana, Pancawati Ariami, Urip Urip, and Fihiruddin Fihiruddin. "Pengaruh Penambahan Glukosa terhadap Pertumbuhan Bakteri Escherichia coli pada Media Pemupuk Nutrient Broth (NB)." JSN : Jurnal Sains Natural 2, no. 2 (2024): 48–52. http://dx.doi.org/10.35746/jsn.v2i2.538.

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Infectious diseases are caused by microorganisms, one of them is Escherichia coli. The process of isolation and identification of bacteria requires enrichment media to increase the number of bacteria. Media Brain Infusion Broth (BHIB) media, the growth of Escherichia coli bacteria is better than Nutrient Broth (NB). Demahui's glucose as a normal source of organic carbon for the growth of Escherichia coli bacteria, while in NB media there is no glucose. This study is quoted to obey the effect of the addition of glucose on the NB fertilizer media on the growth of Escherichia coli bacteria. This

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Lopes, Leonardo Quintana Soares, Pedro Henrique Fortes Guerim, Roberto Christ Vianna Santos, Flavia Kolling Marquezan, and Patrícia Kolling Marquezan. "The influence of different culture media on Pseudomonas aeruginosa, Escherichia coli, and Staphylococcus aureus biofilm formation." Bioscience Journal 39 (August 18, 2023): e39096. http://dx.doi.org/10.14393/bj-v39n0a2023-68631.

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Microorganisms such as Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus are frequently isolated in samples of urinary, blood, intestinal, and respiratory infections, among others. These bacteria are also associated with microbial biofilm formation. E. coli, P. aeruginosa, and S. aureus biofilm infections are particularly hard to manage and often associated with nosocomial problems. This study investigated the influence of different culture media on E. coli, P. aeruginosa, and S. aureus biofilm formation. Bacterial performance was evaluated in brain heart infusion broth, Muel

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Mascarin, Gabriel Moura, Sérgio Batista Alves, and Rogério Biaggioni Lopes. "Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa." Brazilian Archives of Biology and Technology 53, no. 4 (2010): 753–61. http://dx.doi.org/10.1590/s1516-89132010000400002.

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This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was ev

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Steens, Anneke, Natacha Milhano, Ingeborg S. Aaberge, and Didrik F. Vestrheim. "In vitroandin vivocomparison of transport media for detecting nasopharyngeal carriage ofStreptococcus pneumoniae." PeerJ 4 (September 22, 2016): e2449. http://dx.doi.org/10.7717/peerj.2449.

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BackgroundAs a standard method for pneumococcal carriage studies, the World Health Organization recommends nasopharyngeal swabs be transported and stored at cool temperatures in a medium containing skim-milk, tryptone, glucose and glycerol (STGG). An enrichment broth used for transport at room temperature in three carriage studies performed in Norway may have a higher sensitivity than STGG. We therefore compared the mediain vitroandin vivo.MethodsFor thein vitrocomponent, three strains (serotype 4, 19F and 3) were suspended in STGG and enrichment broth. Recovery was compared using latex agglut

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Perdana Rozziansha, Tjut Ahmad, Novitawati Simarmata, Eka Wijayanti, and Ahmad Rafiqi Tantawi. "Development and virulence of Metarhizium anisopliae in several liquid media against Oryctes rhinoceros larvae (Coleoptera: Scarabaeidae)." IOP Conference Series: Earth and Environmental Science 1494, no. 1 (2025): 012028. https://doi.org/10.1088/1755-1315/1494/1/012028.

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Abstract Oryctes rhinoceros is one of the main pests in oil palm plantations, especially during replanting and the immature palm stage. Adults of O. rhinoceros were controlled using pheromone traps and insecticide application. On the other hand, the larvae were controlled with the entomopathogenic fungus Metarhizium anisopliae. This research aimed to determine the effects of several liquid media on the growth, spore production, and virulence of M. anisopliae. The liquid media used were potato dextrose broth (PDB), sabouroud dextrose yeast extract broth (SDYB), yeast peptone glucose broth (YPGB

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Sibero, Mada Triandala, Aninditia Sabdaningsih, Ocky Karna Radjasa, Agus Sabdono, Agus Trianto та Subagiyo Subagiyo. "Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli". Jurnal Kelautan Tropis 22, № 1 (2019): 9. http://dx.doi.org/10.14710/jkt.v0i0.3528.

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The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted

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Sibero, Mada Triandala, Aninditia Sabdaningsih, Ocky Karna Radjasa, Agus Sabdono, Agus Trianto та Subagiyo Subagiyo. "Karakterisasi Senyawa Bioaktif Kapang Laut Trichoderma asperellum MT02 dengan Aktivitas Anti-Extended Spectrum β-Lactamase (ESBL) E. coli". Jurnal Kelautan Tropis 22, № 1 (2019): 9. http://dx.doi.org/10.14710/jkt.v22i1.3528.

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The Trichoderma asperellum MT02 has been reported to has antibacterial activity against the Extended Spectrum β-Lactamase (ESBL) E. coli based on the screening results through an agar plug method. This study aimed to evaluate the antibacterial activity of T. asperellum MT02 and characterize the composition of the bioactive compounds group possessed in its crude extract. The isolate was cultured in the Malt Extract Broth (MEB) media (static, 27 oC, 15 days). The intracellular metabolites from mycelium were extracted using methanol while extracellular metabolites from broth media were extracted

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Goksu, Nebil, Sedef Bengisun, Haluk Ataoglu, Yusuf K. Kemaloglu, and Suat Ozbilen. "Preliminary Report on L-Forms: Possible Role in the Infectious Origin of Secretory Otitis Media." Annals of Otology, Rhinology & Laryngology 103, no. 6 (1994): 434–38. http://dx.doi.org/10.1177/000348949410300603.

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Infection and inflammation of the middle ear cleft are important factors in the pathogenesis of secretory otitis media. Although high percentages of negative cultures are confronted in many studies, strong evidence pointing to the infectious nature of this disease could not be overlooked. Many authors agree about the failure of conventional culture methods in identifying the responsible pathogen or pathogens. Besides, some agents, such as some kinds of antibiotics, lysozyme, and perhaps some undetected materials, are capable of changing bacterial behavior and consequently the clinical course.

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TRUSCOTT, R. B., and W. B. MCNAB. "Comparison of Media and Procedures for the Isolation of Listeria monocytogenes from Ground Beef." Journal of Food Protection 51, no. 8 (1988): 626–28. http://dx.doi.org/10.4315/0362-028x-51.8.626.

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Eight protocols for the isolation of Listeria monocytogenes from meat were used to compare the sensitivity of the Listeria enrichment broth of Donnelly and Baigent (DB) to that of a Listeria test broth (LTB) containing horse serum and Tween 80. In addition to L. monocytogenes, other Listeria spp. were isolated from the majority of lean ground beef samples purchased from 50 retail outlets. Nineteen samples were positive using DB broth while 16 were positive using LTB. This difference was not significant. Neither broth alone recovered all of the 29 positive samples. However, sensitivity was sign

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Jin, Tianru, and R. G. E. Murray. "Further studies of swarmer cell differentiation of Proteus mirabilis PM23: a requirement for iron and zinc." Canadian Journal of Microbiology 34, no. 5 (1988): 588–93. http://dx.doi.org/10.1139/m88-098.

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Proteus mirabilis PM23, unlike other motile strains of the species, differentiates in rich fluid media to form nonseptate filaments resembling the swarmer cells formed on solid media. The swarming activity of PM23 is greater than that of the other strains on solid media and it grows faster than another strain, IM47, in differentiation-supporting broth. This faster growth is not exhibited in broth that does not support differentiation. The differentiation of PM23 in brain–heart infusion broth occurs over a wide range of pH and temperature. Inhibitors of swarming on agar plates (p-nitrophenylgly

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Hirapara, Ishita, and L. V. Ghetiya. "Effect of different solid media and liquid media on growth, sporulation and biomass of Hirsutella thompsonii Fisher." JOURNAL OF ECO-FRIENDLY AGRICULTURE 18, no. 2 (2023): 321–26. http://dx.doi.org/10.48165/jefa.2023.18.02.18.

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The experiment on effect of different solid and liquid media on growth, sporulation and biomass of H. thompsonii was conducted at Bio-control research laboratory, Department of Entomology, Navsari Agricultural University during the year of 2020-21. The result revealed that among the five solid media sabouraud’s dextrose agar+yeast medium supported higher radial growth, sporulation and biomass (wet and dry) of H. thompsonii followed by PDA medium. Five liquid media tested for sporulation and biomass of H. thompsonii, significantly highest sporulation, as well as wet and dry mycelial biomass, wa

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June, Geraldine A., Patricia S. Sherrod, Thomas S. Hammack, R. Miguel Amaguana, and Wallace H. Andrews. "Relative Effectiveness of Selenite Cystine Broth, Tetrathionate Broth, and Rappaport-Vassiliadis Medium for the Recovery of Salmonella from Raw Flesh and Other Highly Contaminated Foods: Precollaborative Study." Journal of AOAC INTERNATIONAL 78, no. 2 (1995): 375–80. http://dx.doi.org/10.1093/jaoac/78.2.375.

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Abstract The effectiveness of selenite cystine (SC) broth, tetrathionate (TT) broth, and Rappaport-Vassiliadis (RV) medium for recovery of Salmonella spp. from 8 highly contaminated foods was determined. RV medium prepared from individual ingredients and incubated at 42° and 43°C was compared with 2 commercial (Difco and Oxoid) media incubated at 42°C. Naturally and artificially contaminated foods were tested under 2 protocols. For Protocol 1, each food was preenriched in the appropriate medium. After incubation, serial 10 fold dilutions of the preenriched foods were inoculated into selective

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Jiwintarum, Yunan, Agrijanti Agrijanti, and Baiq Lilis Septiana. "COLIFORM MOST PROBABLE NUMBER (MPN) WITH VARIETIES OF MEDIA VOLUME LACTOSE BROTH SINGLE STRENGTH (LBSS) AND LACTOSE BROTH DOUBLE STRENGTH (LBDS)." Jurnal Kesehatan Prima 11, no. 1 (2018): 11. http://dx.doi.org/10.32807/jkp.v11i1.17.

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Most Probable Number (MPN) is a test that detects the fermentative nature of Coliform in the sample. The MPN test consists of three stages: presumptive test, confirm test, and completeness test. Each of these tests used LBSS, LBDS and BGLB media with volume between 5 ml - 10 ml. There is the variation of this media volume, whether it will affect the positivity value and MPN value of Coliform, it is a problem that will be answered in this study. The research objective was to find out the influence of media volume variation to the result of Coliform bacterium counting test method of MPN variety

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Sandven, Per, E. Arne HØIby, and Lars Vorland. "EVALUATION OF TWO VACUUM BOTTLE BLOOD CULTURE MEDIA - SUPPLEMENTED PEPTONE BROTH AND BRAIN HEART INFUSION BROTH." Acta Pathologica Microbiologica Scandinavica Series B: Microbiology 95B, no. 1-6 (2009): 257–59. http://dx.doi.org/10.1111/j.1699-0463.1987.tb03122.x.

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Azevedo, Ana, Rita Teixeira-Santos, Fábio M. Carvalho, Luciana C. Gomes, Gabriel A. Monteiro, and Filipe J. Mergulhão. "Influence of Surface Material and Nutrient Conditions on Green Fluorescent Protein Production in Escherichia coli Biofilms." Applied Sciences 14, no. 23 (2024): 11029. http://dx.doi.org/10.3390/app142311029.

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Escherichia coli biofilms have been investigated as a platform for producing recombinant proteins. This study aimed to assess the effect of different surface materials and culture media on E. coli biofilm formation and enhanced Green Fluorescent Protein (eGFP) production. Three culture media with different carbon and nitrogen sources (Lysogeny broth, Terrific broth, and M9ZB broth) were tested in combination with three materials with distinct surface properties (stainless steel, polyvinyl chloride, and silicone rubber). Biofilm formation, specific eGFP production, and plasmid copy number were

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