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Journal articles on the topic 'BSA protein'

Author: Grafiati

Published: 6 June 2025

Last updated: 25 July 2025

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1

Ottnad, E., D. P. Via, H. Sinn, E. Friedrich, R. Ziegler, and H. A. Dresel. "Binding characteristics of reduced hepatic receptors for acetylated low-density lipoprotein and maleylated bovine serum albumin." Biochemical Journal 265, no. 3 (1990): 689–98. http://dx.doi.org/10.1042/bj2650689.

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The binding characteristics of reduced hepatic membrane proteins for acetylated low-density lipoprotein (acetyl-LDL) and maleylated bovine serum albumin (Mal-BSA) have been examined. Two receptor activities were extracted from hepatic membranes in the presence of octyl beta-D-glucoside and beta-mercaptoethanol, and were separated by chromatography on Mal-BSA-Sepharose 4B. The receptors were revealed by ligand blotting. The active binding proteins had apparent molecular masses of 35 and 15 kDa in SDS/polyacrylamide gels. Equilibrium studies with protein-phosphatidylcholine complexes indicated t

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2

Bhatt, Anant Narayan, Yogesh Rai, Amit Verma, et al. "Non-Enzymatic Protein Acetylation by 7-Acetoxy-4-Methylcoumarin: Implications in Protein Biochemistry." Protein & Peptide Letters 27, no. 8 (2020): 736–43. http://dx.doi.org/10.2174/0929866527666200305143016.

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Background: The semi-synthetic acetoxycoumarins are known to acetylate proteins using novel enzymatic Calreticulin Transacetylase (CRTAase) system in cells. However, the nonenzymatic protein acetylation by polyphenolic acetates is not known. Objective: To investigate the ability of 7-acetoxy-4-methyl coumarin (7-AMC) to acetylate proteins non-enzymatically in the test tube. Methods: We incubated 7-AMC with BSA and analyzed the protein acetylation using Western blot technique. Further, BSA induced biophysical changes in the spectroscopic properties of 7-AMC was analyzed using Fluorescence spect

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3

Nomani, Alireza, Hamed Nosrati, Hamidreza Manjili, Leila Khesalpour, and Hossein Danafar. "Preparation and Characterization of Copolymeric Polymersomes for Protein Delivery." Drug Research 67, no. 08 (2017): 458–65. http://dx.doi.org/10.1055/s-0043-106051.

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AbstractBiodegradable copolymeric polymersomes have been used for controlled drug delivery of proteins. These polymersomes important areas to overcome formulation associated problems of the proteins. The aim of this study was to develop polymersomes using biodegradable copolymers for delivery of bovine serum albumin (BSA) as a model protein. Encapsulated BSA by mPEG-PCL polymersomes led to formation of BSA-loaded mPEG-PCL polymersomes. The polymersomes synthesized with the protein-polymer ratio of 1:4 at 15 000 rpm gave maximum loading, minimum polydispersion with maximally sustained protein r

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4

Palacio, L., C. C. Ho, P. Prádanos, A. Hernández, and A. L. Zydney. "Fouling with protein mixtures in microfiltration: BSA–lysozyme and BSA–pepsin." Journal of Membrane Science 222, no. 1-2 (2003): 41–51. http://dx.doi.org/10.1016/s0376-7388(03)00143-1.

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5

Guan, D., and G. M. Green. "Significance of peptic digestion in rat pancreatic secretory response to dietary protein." American Journal of Physiology-Gastrointestinal and Liver Physiology 271, no. 1 (1996): G42—G47. http://dx.doi.org/10.1152/ajpgi.1996.271.1.g42.

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The importance of peptic digestion of dietary protein in pancreatic enzyme secretion and cholecystokinin (CCK) release was investigated in conscious rats. Native casein and native bovine serum albumin (BSA) were infused intragastrically and intra-intestinally, and the effect of peptic predigestion on the pancreatic secretory and plasma CCK responses to BSA were determined (all infused at 450 mg/h). When dietary proteins were infused intraduodenally, native casein was a much stronger stimulant of CCK release (5.8 +/- 0.6 vs. 1.6 +/- 0.2 pM) and pancreatic protein secretion [5,592 +/- 736 vs. 75

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6

Lv, Ying Hai, Gui Jiang Li, Li Qiang Cui, Hua Xiao Yan, and Shi Xue Zhou. "The Study of Existential State of Protein in Protein/Montmorillonite Complexes." Advanced Materials Research 194-196 (February 2011): 1647–51. http://dx.doi.org/10.4028/www.scientific.net/amr.194-196.1647.

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The existential state of protein in complexes directly affects the performance and applications of the composite materials. The interlayer space changes of montmorillonite in the protein / montmorillonite (MMT) composite were identified by X-ray diffraction (XRD). And the interaction between protein and MMT were analyzed by Fourier transform infrared spectrometry (FT-IR) and UV/vis spectrophotometry. The loading amount of bovine serum albumin (BSA) onto MMT was calculated from the TG data. The types of adsorption isotherm of BSA onto montmorillonite were analyzed. From the above analysis, it c

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7

Deffebach, M. E., C. J. Bryan, and C. M. Hoy. "Protein movement across cultured guinea pig trachea: specificity and effect of transcytosis inhibitors." American Journal of Physiology-Lung Cellular and Molecular Physiology 271, no. 5 (1996): L744—L752. http://dx.doi.org/10.1152/ajplung.1996.271.5.l744.

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Airway surface liquid (ASL) is a complex fluid with solutes including electrolytes, lipids, mucins, and proteins. The proximal airways are absorptive for most solutes, including proteins. We investigated the process of protein movement across confluent primary cultures of guinea pig trachea grown on filters using fluorescent-labeled bovine serum albumin (BSA), ovalbumin (OA), and 70-kDa dextran (Dex). We found marked asymmetry of BSA and OA transepithelial flux, with apical-to-basolateral flux (JA-->B) 10 times greater than the opposite direction (JB-->A) for both proteins. The apparent

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8

Yu, Jing, Yun Chen, Liqun Xiong, Xiaoyue Zhang, and Yue Zheng. "Conductance Changes in Bovine Serum Albumin Caused by Drug-Binding Triggered Structural Transitions." Materials 12, no. 7 (2019): 1022. http://dx.doi.org/10.3390/ma12071022.

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Proteins, due to their binding selectivity, are promising candidates for fabricating nanoscale bio-sensors. However, the influence of structural change on protein conductance caused by specific protein-ligand interactions and disease-induced degeneration still remains unknown. Here, we excavated the relationship between circular dichroism (CD) spectroscopy and conductive atomic force microscopy (CAFM) to reveal the effect of the protein secondary structures changes on conductance. The secondary structure of bovine serum albumin (BSA) was altered by the binding of drugs, like amoxicillin (Amox)

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9

Gekle, M., S. Mildenberger, R. Freudinger, and S. Silbernagl. "Long-term protein exposure reduces albumin binding and uptake in proximal tubule-derived opossum kidney cells." Journal of the American Society of Nephrology 9, no. 6 (1998): 960–68. http://dx.doi.org/10.1681/asn.v96960.

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To avoid renal loss of large amounts of proteins, filtered proteins are reabsorbed by endocytosis along the proximal tubule. However, although protein reabsorption is a task of proximal tubular cells, it is also a threat because it may cause cell injury. This study determines whether exposure to bovine serum albumin (BSA) leads to regulatory changes in endocytosis of FITC-BSA in proximal tubule-derived opossum kidney cells. Preincubation with BSA led to a decrease of FITC-BSA endocytosis with an IC50 value of 0.58 g/L. Specific binding of FITC-BSA to the apical membrane was also reduced (IC50

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10

Alhazmi, Hassan. "FT-IR Spectroscopy for the Identification of Binding Sites and Measurements of the Binding Interactions of Important Metal Ions with Bovine Serum Albumin." Scientia Pharmaceutica 87, no. 1 (2019): 5. http://dx.doi.org/10.3390/scipharm87010005.

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Proteins play crucial roles in the transportation and distribution of therapeutic substances, including metal ions in living systems. Some metal ions can strongly associate, while others show low affinity towards proteins. Consequently, in the present work, the binding behaviors of Ca2+, Ba2+, Ag+, Ru3+, Cu2+ and Co2+ with bovine serum albumin (BSA) were screened. BSA and the metal ions were allowed to interact at physiological pH and their binding interactions were screened by using FT-IR spectroscopy. Spectra were collected by using hydrated films over a range of 4000–400 cm−1. The interacti

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11

Luwei Tian, Luwei Tian, Ming Guo Ming Guo, and Xingtao Xu and Linfang Shi Xingtao Xu and Linfang Shi. "Imaging Serum Proteome Behavior in Process of Lead Transportation in Vivo: A Fluorescence Spectroscopic Analysis Insight." Journal of the chemical society of pakistan 44, no. 5 (2022): 453. http://dx.doi.org/10.52568/001122/jcsp/44.05.2022.

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As a common heavy metal, Pb (II) can react with biological macromolecules in the human body and have an impact on human health, but there are few studies on its synergistic interaction with a variety of proteins of different abundance. Pb (II) binding with a synthetic model protein system (bovine serum albumin (BSA) and bovine lactoferrin (BLF)) was characterized using fluorescence spectroscopy and was described using a quantitative model. Pb (II) quenched the fluorescence of BSA-BLF, indicating that Pb (II) interacted with the BSA-BLF protein system, and was affected by single protein, mixed

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12

Ramzy, Esraa, Mohamed M. Ibrahim, Ibrahim M. El-Mehasseb, et al. "Synthesis, Biophysical Interaction of DNA/BSA, Equilibrium and Stopped-Flow Kinetic Studies, and Biological Evaluation of bis(2-Picolyl)amine-Based Nickel(II) Complex." Biomimetics 7, no. 4 (2022): 172. http://dx.doi.org/10.3390/biomimetics7040172.

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Reaction of bis(2-picolyl)amine (BPA) with Ni(II) salt yielded [(BPA)NiCl2(H2O)] (NiBPA). The Ni(II) in NiBPA bound to a BPA ligand, two chloride, and one aqua ligands. Because most medications inhibit biological processes by binding to a specific protein, the stopped-flow technique was used to investigate DNA/protein binding in-vitro, and a mechanism was proposed. NiBPA binds to DNA/protein more strongly than BPA via a static quenching mechanism. Using the stopped-flow technique, a mechanism was proposed. BSA interacts with BPA via a fast reversible step followed by a slow irreversible step,

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13

Thomas, Mark E., Kevin P. G. Harris, John Walls, Peter N. Furness, and Nigel J. Brunskill. "Fatty acids exacerbate tubulointerstitial injury in protein-overload proteinuria." American Journal of Physiology-Renal Physiology 283, no. 4 (2002): F640—F647. http://dx.doi.org/10.1152/ajprenal.00001.2002.

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The role of the albumin-carried fatty acids in the induction of tubulointerstitial injury was studied in protein-overload proteinuria. Rats were injected with fatty acid-carrying BSA [FA(+)BSA], fatty acid-depleted BSA [FA(−)BSA], or saline. Macrophage infiltration was measured by immunohistochemical staining, apoptotic cells were detected by in situ end labeling, and proliferating cells were identified by in situ hybridization for histone mRNA. Macrophage infiltration was significantly greater in the FA(+)BSA group than in the FA(−)BSA and saline groups. The infiltrate was largely restricted

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14

Wang, Guorong, Ning Liu, and Mingruo Guo. "Use of Whey Protein as a Natural Polymer for Tissue Adhesive: Preliminary Formulation and Evaluation In Vitro." Polymers 10, no. 8 (2018): 843. http://dx.doi.org/10.3390/polym10080843.

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The use of sutures is still the most widely practiced solution for wound closure and tissue reconstruction; however, scarring is a common defect resulting from sutures on topical use. In some cases, the conventional sutures are unable to seal the sites where fluid and air leakage could occur. Tissue adhesives though have lower tensile strength than sutures, may make scarless surgery possible, or prevent fluid and air leakage. A product called BioGlue® (CryoLife Inc, Kennesaw, GA, USA), based on bovine serum albumin (BSA, a protein) and glutaraldehyde (GTA, crosslinker), has been approved for c

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15

Chen, Shu-Ting, Sumith Ranil Wickramasinghe, and Xianghong Qian. "Electrospun Hydrophobic Interaction Chromatography (HIC) Membranes for Protein Purification." Membranes 12, no. 7 (2022): 714. http://dx.doi.org/10.3390/membranes12070714.

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Responsive membranes for hydrophobic interaction chromatography have been fabricated by functionalizing poly(N-vinylcaprolactam) (PVCL) ligands on the substrate of electrospun regenerated cellulose nanofibers. Both static and dynamic binding capacities and product recovery were investigated using bovine serum albumin (BSA) and Immunoglobulin G (IgG) as model proteins. The effects of ligand chain length and chain density on static binding capacity were also studied. A static binding capacity of ~25 mg/mL of membrane volume (MV) can be achieved in optimal ligand grafting conditions. For dynamic

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16

Vlassara, H., M. Brownlee, and A. Cerami. "Novel macrophage receptor for glucose-modified proteins is distinct from previously described scavenger receptors." Journal of Experimental Medicine 164, no. 4 (1986): 1301–9. http://dx.doi.org/10.1084/jem.164.4.1301.

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A high-affinity macrophage receptor has been identified that recognizes proteins modified by a common in vivo process, long-term nonenzymatic reaction of glucose with proteins (AGE proteins). This receptor for glucose-modified proteins is now shown to be distinct from previously described scavenger receptors, using competition and crosscompetition experiments between AGE-modified protein and a variety of in vitro-modified scavenger receptor ligands, including unmodified BSA, unmodified low-density lipoproteins (LDL), acetyl-LDL, maleyl-BSA, and formaldehyde-treated BSA. Furthermore, the specif

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17

Oldfield, David J., Harjinder Singh, and Mike W. Taylor. "Kinetics of heat-induced whey protein denaturation and aggregation in skim milks with adjusted whey protein concentration." Journal of Dairy Research 72, no. 3 (2005): 369–78. http://dx.doi.org/10.1017/s002202990500107x.

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Microfiltration and ultrafiltration were used to manufacture skim milks with an increased or reduced concentration of whey proteins, while keeping the casein and milk salts concentrations constant. The skim milks were heated on a pilot-scale UHT plant at 80, 90 and 120 °C. The heat-induced denaturation and aggregation of β-lactoglobulin (β-lg), α-lactalbumin (α-la) and bovine serum albumin (BSA) were quantified by polyacrylamide gel electrophoresis. Apparent rate constants and reaction orders were calculated for β-lg, α-la and BSA denaturation. Rates of β-lg, α-la and BSA denaturation increase

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18

HAVEA, PALATASA, HARJINDER SINGH та LAWRENCE K. CREAMER. "Characterization of heat-induced aggregates of β-lactoglobulin, α-lactalbumin and bovine serum albumin in a whey protein concentrate environment". Journal of Dairy Research 68, № 3 (2001): 483–97. http://dx.doi.org/10.1017/s0022029901004964.

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Bovine β-lactoglobulin (β-lg), α-lactalbumin (α-la) and bovine serum albumin (BSA), dispersed in ultrafiltration permeate, that had been prepared from whey protein concentrate solution (100 g/kg, pH 6·8), were heated at 75 °C. The consequent protein aggregation was studied by one-dimensional (1D) and two-dimensional (2D) polyacrylamide gel electrophoresis (PAGE). When 100 g β-lg/kg permeate solution was heated at 75 °C, cooled and examined, large aggregates were observed. These aggregates were partially dissociated in SDS solution to give monomers, disulphide-bonded dimers, trimers and larger

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19

Wagener, Victoria, Anne-Sophie Faltz, Manuela S. Killian, Patrik Schmuki, and Sannakaisa Virtanen. "Protein interactions with corroding metal surfaces: comparison of Mg and Fe." Faraday Discussions 180 (2015): 347–60. http://dx.doi.org/10.1039/c4fd00253a.

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The influence of bovine serum albumin (BSA) on the electrochemical behaviour of pure Mg and Fe was studied in simulated body fluid (SBF), in view of the possible application of these materials as biodegradable metals. Results indicate a different trend for the BSA-effect on corrosion for the two metals: for Mg, a strong corrosion-inhibiting effect is observed in the presence of BSA in solution, especially for short-term exposure, whereas for Fe only a slight acceleration of corrosion is caused by the addition of BSA to the solution. For both metals, the protein-effect on the electrochemical be

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20

Gottlieb, R. A., W. J. Lennarz, R. D. Knowles, et al. "Synthetic peptide corresponding to a conserved domain of the retroviral protein p15E blocks IL-1-mediated signal transduction." Journal of Immunology 142, no. 12 (1989): 4321–28. http://dx.doi.org/10.4049/jimmunol.142.12.4321.

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Abstract We studied the mode of action of the synthetic peptide CKS-17, which is a heptadecapeptide homologous to a highly conserved region of the immunosuppressive retroviral envelope protein p15E, as well as to envelope proteins of the human T cell leukemia virus I and II. Previous studies have established that CKS-17 conjugated to BSA (CKS-17-BSA) inhibited IL-1-mediated tumor toxicity in melanoma cells and proliferation in murine Th clones. We examined the effects of CKS-17-BSA on IL-1 action. CKS-17-BSA did not bind to IL-1, nor did it affect the number of IL-1 receptors, their binding af

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21

Hoque, E., M. K. Biswas, H. M. Syfuddin, S. M. Sharafuddin, S. K. Das, and Y. Haque. "Nonlinear optical characteristic curve of protein (BSA)." Journal of Optics 49, no. 3 (2020): 392–96. http://dx.doi.org/10.1007/s12596-020-00631-5.

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22

Nagai, Satoshi, Kanji Tsuru, Satoshi Hayakawa, and Akiyoshi Osaka. "Conformational Change of Protein due to Contact with Bioceramic Materials." Key Engineering Materials 284-286 (April 2005): 517–20. http://dx.doi.org/10.4028/www.scientific.net/kem.284-286.517.

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The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al2O3, SiO2, TiO2, ZrO2, hydroxyapatite and b-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO2, ZrO2 and Al2O3, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO2 and ZrO2. We discussed t

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23

Sun, Lin, Ananya Srinivas, and Ron C. Runnebaum. "Understanding the Impact of Key Wine Components on the Use of a Non-Swelling Ion-Exchange Resin for Wine Protein Fining Treatment." Molecules 26, no. 13 (2021): 3905. http://dx.doi.org/10.3390/molecules26133905.

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The impact of key classes of compounds found in wine on protein removal by the ion-exchange resin, Macro-Prep® High S, was examined by adsorption isotherm experiments. A model wine system, which contained a prototypical protein Bovine Serum Albumin (BSA), was used. We systematically changed concentrations of individual chemical components to generate and compare adsorption isotherm plots and to quantify adsorption affinity or capacity parameters of Macro-Prep® High S ion-exchange resin. The pH (hydronium ion concentration), ethanol concentration, and prototypical phenolics and polysaccharide c

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24

Weizhong, Ke, and Wu Jianzhong. "Influence of the Nitrobenzene Extraction Technique on the Protein Molecule Conformation." Applied Spectroscopy 48, no. 2 (1994): 209–13. http://dx.doi.org/10.1366/0003702944028506.

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The Raman spectra of lysozyme, ribonuclease, and BSA (bovine serum albumin) in aqueous solution extracted by nitrobenzene and the ultraviolet visible absorption spectra of BSA in aqueous solution have been studied. It has been proven that the structure of all the characteristic Raman and UV bands of BAS protein in aqueous solution is due to α;-helical and random-coil conformations. The tyrosine residue is “exposed”, and the conformation of the carbon atoms in the disulfide bridge c-c-s-s-c-c is trans-gauche-gauche. The influence of adding nitrobenzene to lysozyme, ribonuclease, and BSA aqueous

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25

Tessler, Lee A., Casey D. Donahoe, Daniel J. Garcia, Young-Shin Jun, Donald L. Elbert, and Robi D. Mitra. "Nanogel surface coatings for improved single-molecule imaging substrates." Journal of The Royal Society Interface 8, no. 63 (2011): 1400–1408. http://dx.doi.org/10.1098/rsif.2010.0669.

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Surfaces that resist protein adsorption are important for many bioanalytical applications. Bovine serum albumin (BSA) coatings and multi-arm poly(ethylene glycol) (PEG) coatings display low levels of non-specific protein adsorption and have enabled highly quantitative single-molecule (SM) protein studies. Recently, a method was developed for coating a glass with PEG–BSA nanogels, a promising hybrid of these two low-background coatings. We characterized the nanogel coating to determine its suitability for SM protein experiments. SM adsorption counting revealed that nanogel-coated surfaces exhib

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26

Kowapradit, J., Theerasak Rojanarata, Tanasait Ngawhirunpat, A. Apirakaramwong, Warayuth Sajomsang, and Praneet Opanasopit. "Application of Methylated N-(4-N,N-Dimethylaminocinnamyl) Chitosan for Oral Protein Drug Delivery." Advanced Materials Research 506 (April 2012): 465–68. http://dx.doi.org/10.4028/www.scientific.net/amr.506.465.

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In the present study, methylated N-(4-N,N-dimethylaminocinnamyl) chitosan (TM65CM50CS) was synthesized and investigated for oral protein drug delivery by combining with liposomes entrapped bovine serum albumin (FITC-BSA), a model protein. FITC-BSA liposomes composed of egg yolk phosphatidylcholine and sodium oleate in molar ratio of 10:2 were prepared by thin film hydration method. The TM65CM50CS coated liposomal FITC-BSA was evaluated for transport of protein and its cytotoxicity in Caco-2 cells. Moreover, the in vitro stability of BSA in TM65CM50CS coated liposomes was also examined by the d

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27

Pathak, Jyotsana, Kamla Rawat, V. K. Aswal, and H. B. Bohidar. "Interactions in globular proteins with polyampholyte: coacervation route for protein separation." RSC Advances 5, no. 18 (2015): 13579–89. http://dx.doi.org/10.1039/c4ra13133a.

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28

Liu, Yong, Yan Sun, Yan Li Li, Shao Chun Xu, and Yao Xing Xu. "Characteration of Protein Loaded Chitosan Nanoparticles at Different pH Values." Advanced Materials Research 284-286 (July 2011): 950–53. http://dx.doi.org/10.4028/www.scientific.net/amr.284-286.950.

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In this paper, the properties of chitosan (CS) nanoparticles (CS NPs) and Bovine Serum Albumin (BSA) loaded chitosan nanoparticles (BSA-loaded CS NPs) prepared with sodium tripolyphosphate (TPP) at different pH values were discussed. In addition, BSA encapsulation efficiency, zeta potentials and size of CS NPs and BSA-loaded CS NPs were characterized. The results indicated that sizes of CS NPs presented “V” change along with increase of pH values, while the size of BSA-loaded CS NPs showed “U” shape change. BSA encapsulation efficiency reached the biggest value when the pH value was 5.5. Zeta

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29

Lee, D., K. Gleich, S. A. Fraser, M. Katerelos, P. F. Mount, and D. A. Power. "Limited capacity of proximal tubular proteolysis in mice with proteinuria." American Journal of Physiology-Renal Physiology 304, no. 7 (2013): F1009—F1019. http://dx.doi.org/10.1152/ajprenal.00601.2012.

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Albuminuria is associated with the additional loss in the urine of small molecular weight proteins normally degraded by the proximal convoluted tubule (PCT), and competition for binding to the megalin/cubilin reuptake system has been considered the likely cause. We have previously reported that deficiency of the intrinsic lysosomal protein Limp-2 causes tubular proteinuria due to reduced fusion of endosomes with lysosomes in the PCT leading to inadequate proteolysis. To determine whether this mechanism also contributes to the tubular proteinuria induced by albumin overload in normal mice, wild

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Michaud, N., and D. S. Goldfarb. "Multiple pathways in nuclear transport: the import of U2 snRNP occurs by a novel kinetic pathway." Journal of Cell Biology 112, no. 2 (1991): 215–23. http://dx.doi.org/10.1083/jcb.112.2.215.

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Protein import to the nucleus is a signal-mediated process that exhibits saturation kinetics. We investigated whether signal bearing proteins compete with U2 and U6 snRNPs during import. When injected into Xenopus oocytes, saturating concentrations of P(Lys)-BSA, a protein bearing multiple nuclear localization signals from SV40 large T-antigen, reduce the rate of [125I]P(Lys)-BSA and of [125I]nucleoplasmin import, consistent with their competing for and sharing the same limiting component of the import apparatus. In contrast, saturating concentrations of P(Lys)-BSA do not reduce the rate of He

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31

de Souza, Carmen Regina, Maurício Ikeda Yoguim, Nathalia Mariana Pavan, Nelson Henrique Morgon, Valdecir Farias Ximenes, and Aguinaldo Robinson de Souza. "Evidence of the Differences Between Human and Bovine Serum Albumin Through the Interaction with Coumarin-343: Experimental (ICD) and Theoretical Studies (DFT and Molecular Docking)." Biophysica 5, no. 3 (2025): 27. https://doi.org/10.3390/biophysica5030027.

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Coumarins are known for interacting with proteins and exhibiting diverse biological activities. This study investigates the interaction between coumarin-343 (C343) and human (HSA) and bovine (BSA) serum albumins. Fluorescence spectroscopy and theoretical simulations, including density functional theory (DFT) and molecular docking, were used to analyze the ligand–protein complex formation. The fluorescence quenching data revealed that C343 binds to both proteins, with binding constants of 2.1 × 105 mol·L−1 (HSA) and 6.5 × 105 mol·L−1 (BSA), following a 1:1 stoichiometry. Binding site markers id

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32

K. Rochani, Ankit, Sivakumar Balasubramanian, Aswathy Ravindran Girija, Toru Maekawa, Gagan Kaushal, and D. Sakthi Kumar. "Heat Shock Protein 90 (Hsp90)-Inhibitor-Luminespib-Loaded-Protein-Based Nanoformulation for Cancer Therapy." Polymers 12, no. 8 (2020): 1798. http://dx.doi.org/10.3390/polym12081798.

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Drugs targeting heat shock protein 90 (Hsp90) have been extensively explored for their anticancer potential in advanced clinical trials. Nanoformulations have been an important drug delivery platform for the anticancer molecules like Hsp90 inhibitors. It has been reported that bovine serum albumin (BSA) nanoparticles (NPs) serve as carriers for anticancer drugs, which have been extensively explored for their therapeutic efficacy against cancers. Luminespib (also known as NVP-AUY922) is a new generation Hsp90 inhibitor that was introduced recently. It is one of the most studied Hsp90 inhibitors

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Zeeshan, Farrukh, Misbah Tabbassum, Lene Jorgensen, and Natalie J. Medlicott. "Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy as an Analytical Method to Investigate the Secondary Structure of a Model Protein Embedded in Solid Lipid Matrices." Applied Spectroscopy 72, no. 2 (2017): 268–79. http://dx.doi.org/10.1177/0003702817739908.

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Protein drugs may encounter conformational perturbations during the formulation processing of lipid-based solid dosage forms. In aqueous protein solutions, attenuated total reflection Fourier transform infrared (ATR FT-IR) spectroscopy can investigate these conformational changes following the subtraction of spectral interference of solvent with protein amide I bands. However, in solid dosage forms, the possible spectral contribution of lipid carriers to protein amide I band may be an obstacle to determine conformational alterations. The objective of this study was to develop an ATR FT-IR spec

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34

Kato, Katsuya, Sungho Lee, and Fukue Nagata. "Preparation of Protein–Peptide–Calcium Phosphate Composites for Controlled Protein Release." Molecules 25, no. 10 (2020): 2312. http://dx.doi.org/10.3390/molecules25102312.

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Protein–peptide–calcium phosphate composites were developed for achieving sustainable and controlled protein release. Bovine serum albumin (BSA) as a model acidic protein was efficiently encapsulated with basic polypeptides such as polylysine and polyarginine during the precipitation of calcium phosphate (CaP). The prepared composites were fully characterized in terms of their morphologies, crystallinities, and the porosity of their structures, and from these analyses, it was observed that there are no significant differences between the composites. Scanning transmission electron microscopy an

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Davidsson, Lena, Annette Almgren, Brittmarie SandströM, Marc El-A. Juillerat, and Richard F. Hurrell. "Zinc absorption in adult humans: the effect of protein sources added to liquid test meals." British Journal of Nutrition 75, no. 4 (1996): 607–13. http://dx.doi.org/10.1079/bjn19960163.

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The influence of different protein sources on Zn absorption was evaluated in healthy adults by radioisotopic labelling of single meals, followed by whole-body retention measurements 14 d after intake. Semi-synthetic liquid diets were used for the evaluation of different animal-protein sources and dephytinized soyabean-protein isolate ( ··01 g phytic acid/kg). Zn absorption was measured in the same subjects from identical test meals containing no added protein. No statistically significant differences were found in the Zn absorption from test meals containing bovine whey, casein or egg albumen

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Naddaf, A. A., H. J. Bart, and I. Tsibranska. "Diffusion Kinetics of BSA Protein in Stimuli Responsive Hydrogels." Defect and Diffusion Forum 297-301 (April 2010): 664–69. http://dx.doi.org/10.4028/www.scientific.net/ddf.297-301.664.

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A two-dimensional diffusion mathematical model with moving boundary conditions was developed to evaluate the diffusion kinetics of bovine serum albumin (BSA) through the network of poly(N-isopropylacrylamide) hydrogel (poly(NIPAAm)). These thermosensitive hydrogels were experimentally tested for their response to BSA by exposing the hydrogel disc-shaped geometry to different temperatures and varied protein concentration. The BSA release, which is coupled with hydrogel shrinking when reaching the low critical solution temperature (LCST) of poly(NIPAAm), could be satisfactory described by the mo

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EVA, TVRDÁ, HASHIM FARIDULLAH, LUKÁČOVÁ JANA, MASSÁNYI PETER, GOC ZOFIA, and LUKÁČ NORBERT. "THE EFFECT OF BOVINE SERUM ALBUMIN IN CULTURE MEDIA ON BOVINE SPERMATOZOA MOTILITY PARAMETERS IN VITRO." Contemporary Agriculture (2013) 62, no. 3-4 (2013): 139–48. https://doi.org/10.5281/zenodo.7256940.

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The aim of this study was to analyze the influence of bovine serum albumin (BSA) used in different culture media on motility and viability of bovine spermatozoa during a 24-hour in vitro cultivation. We compared native samples (N) and samples cultivated in a commercial egg yolk medium (R) with samples cultivated in several experimental media containing 10% or 20% BSA. The analysis was carried out during three time periods (time 0, time 1 hour and time 24 hours) using Sperm VisionTM CASA system. Our study shows an obvious time-dependent decrease of the spermatozoa viability parameters in all ex

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Johnston, P. A., M. M. Jansen, S. D. Somers, D. O. Adams, and T. A. Hamilton. "Maleyl-BSA and fucoidan induce expression of a set of early proteins in murine mononuclear phagocytes." Journal of Immunology 138, no. 5 (1987): 1551–58. http://dx.doi.org/10.4049/jimmunol.138.5.1551.

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Abstract We examined the effect of maleyl-BSA on specific protein expression in murine peritoneal macrophages by radiolabeling treated macrophages with [35S]methionine followed by SDS-polyacrylamide gel electrophoresis. Such treatment induces the expression of a set of at least seven proteins (38, 42, 57, 65, 75, 80, and 85 kD). A similar set of proteins is also induced by treatment of macrophages with the algal polysaccharide fucoidan. The proteins resemble those induced in response to treatment of this same cell population with bacterial lipopolysaccharide (LPS), as judged by co-migration in

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Renaudin, Sarda, Campillo-Gimenez, et al. "Adsorption of Proteins on m-CPPD and Urate Crystals Inhibits Crystal-induced Cell Responses: Study on Albumin-crystal Interaction." Journal of Functional Biomaterials 10, no. 2 (2019): 18. http://dx.doi.org/10.3390/jfb10020018.

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The biological effects and cellular activations triggered by monosodium urate (MSU) and calcium pyrophosphate dihydrate (monoclinic: m-CPPD) crystals might be modulated by protein coating on the crystal surface. This study is aimed at: (i) Identifying proteins adsorbed on m-CPPD crystals, and the underlying mechanisms of protein adsorption, and (ii) to understand how protein coating did modulate the inflammatory properties of m-CPPD crystals. The effects of protein coating were assessed in vitro using primary macrophages and THP1 monocytes. Physico-chemical studies on the adsorption of bovine

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Ponkshe, Pranav, Yingzhe Wang, and Chalet Tan. "Systemic Protein Delivery via Inhalable Liposomes: Formulation and Pharmacokinetics." Pharmaceutics 15, no. 7 (2023): 1951. http://dx.doi.org/10.3390/pharmaceutics15071951.

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The enormous and thin alveolar epithelium is an attractive site for systemic protein delivery. Considering the excellent biocompatibility of phospholipids with endogenous pulmonary surfactant, we engineered dimyristoylphosphatidylcholine (DMPC)-based liposomes for pulmonary administration, using Cy5.5-labeled bovine serum albumin (BSA-Cy5.5) as a model protein payload. The level of cholesterol (Chol) and surface modification with PEG in inhalable liposomes were optimized iteratively based on the encapsulation efficiency, the release kinetics in the simulated lung fluid, and the uptake in murin

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Lee, Wing-Hin, Ching-Yee Loo, Kim Linh Van, Alexander V. Zavgorodniy, and Ramin Rohanizadeh. "Modulating protein adsorption onto hydroxyapatite particles using different amino acid treatments." Journal of The Royal Society Interface 9, no. 70 (2011): 918–27. http://dx.doi.org/10.1098/rsif.2011.0586.

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Hydroxyapatite (HA) is a material of choice for bone grafts owing to its chemical and structural similarities to the mineral phase of hard tissues. The combination of osteogenic proteins with HA materials that carry and deliver the proteins to the bone-defective areas will accelerate bone regeneration. The study investigated the treatment of HA particles with different amino acids such as serine (Ser), asparagine (Asn), aspartic acid (Asp) and arginine (Arg) to enhance the adsorption ability of HA carrier for delivering therapeutic proteins to the body. The crystallinity of HA reduced when ami

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Tang, Qiu Shi, Hong Tao Lei, Long Bin Huang, Yuan Ming Sun, and Jin Yi Yang. "Effect of Carrier Surface Modification on the Resultant Antibody to Folic Acid." Advanced Materials Research 581-582 (October 2012): 77–80. http://dx.doi.org/10.4028/www.scientific.net/amr.581-582.77.

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For the generation of antibodies against small hapten molecules, the hapten is cross-linked with some carrier protein to make it immunogenic. However, it was rarely systematically studied about the effect of modified carrier protein on the obtained antibody nature. In this study, folic acid as the model hapten was coupled to natural and surface-modified bovine serum albumin (BSA) using modifying agent amidocaproic acid (ACA) and hexamethylene diamine (HDA). The three immunogens of FA-BSA, FA-ACA-BSA, FA-HDA-BSA were confirmed by SDS-PAGE and ultraviolet spectrum (UV). The Balb/C mice were immu

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George, F., D. Kerschen, A. Van Nuffel, J. F. Rees, and I. Donnay. "Plant protein hydrolysates (plant peptones) as substitutes for animal proteins in embryo culture medium." Reproduction, Fertility and Development 21, no. 4 (2009): 587. http://dx.doi.org/10.1071/rd08147.

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The aim of the present study was to improve the sanitary quality of in vitro-produced bovine embryos by using plant protein hydrolysates (plant peptones) as substitutes for animal proteins. Peptones were compared with bovine serum albumin (BSA) as the protein source in synthetic oviduct fluid medium and the quality of the resulting embryos was determined. Two batches of peptones (wheat and cotton) were selected on the basis of their anti-oxidant properties. When added to the culture medium, both peptones (at 0.56 mg mL–1 for cotton peptone and at 0.18 mg mL–1 for wheat peptone) led to similar

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Sasongko, Wahidin Teguh, Lies Mira Yusiati, Zaenal Bachruddin, and Mugiono (Mugiono). "Optimalisasi Pengikatan Tanin Daun Nangka dengan Protein Bovine Serum Albumin (Optimalisation Binding of Jackfruit Leaves Tannin with Bovine Serum Albumin Protein)." Buletin Peternakan 34, no. 3 (2012): 154. http://dx.doi.org/10.21059/buletinpeternak.v34i3.84.

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<p>Tannins are high molecular weight polyphenol compounds with ability to bind proteins. Based on the structure,<br />albumin are simple globular molecule protein. Optimalisation binding of jackfruit leave tannins to bovine serum (BSA)<br />albumin was done in two stages. The first stage was to determine levels of tannins and condensed tannins in jackfruit leaves grown in mediterranean soil types. Second research was optimalisation binding of jackfruit leaf tannins with<br />bovine serum albumin. In the second stage there was the determination of protein-percipitable ph

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Lee, Hyun Soon, Kyung Chul Moon, Chi Young Song, Bong Cho Kim, Suxia Wang, and Hye Kyoung Hong. "Glycated albumin activates PAI-1 transcription through Smad DNA binding sites in mesangial cells." American Journal of Physiology-Renal Physiology 287, no. 4 (2004): F665—F672. http://dx.doi.org/10.1152/ajprenal.00034.2004.

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Amadori-modified glycated albumin stimulates extracellular matrix and transforming growth factor-β (TGF-β) expression in cultured mesangial cells. Smad proteins transduce the TGF-β-mediated signal, and Smad-binding CAGA sequences are present in the plasminogen activator inhibitor-1 (PAI-1) promoter. This study examined whether glycated albumin induces PAI-1 transcription in human mesangial cells (HMC) through Smad-binding sites in the PAI-1 promoter. Quiescent HMC were exposed to 200 μg/ml bovine serum albumin (BSA) or glycated BSA (Gly-BSA) for 12–72 h. At 24 h, Gly-BSA stimulated TGF-β1 and

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Zhang, Liangliang, Qinhao Guan, He Zhang, and Lihua Tang. "Effect of Metal Ions on the Interaction of Condensed Tannins with Protein." Foods 12, no. 4 (2023): 829. http://dx.doi.org/10.3390/foods12040829.

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A quantitative analysis of the precipitate effects of metal ions (Al3+, Fe2+, Cu2+, Zn2+) by bovine serum albumin (BSA) on two condensed tannins (CT) from sorghum and plum was presented in this study. The results showed that adding metal ions enhanced the precipitation of proteins by CT, depending on the type and concentration of the metal ions used in the reaction system. The presence of metal ions and precipitation results on the CT–protein complex showed that Al3+ and Fe2+ had a higher binding ability with CT and a weaker influence on the precipitation of the CT–protein complex than Cu2+ an

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Gottlieb, R. A., E. S. Kleinerman, C. A. O'Brian, S. Tsujimoto, G. J. Cianciolo, and W. J. Lennarz. "Inhibition of protein kinase C by a peptide conjugate homologous to a domain of the retroviral protein p15E." Journal of Immunology 145, no. 8 (1990): 2566–70. http://dx.doi.org/10.4049/jimmunol.145.8.2566.

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Abstract Retroviral infection is associated with immunosuppression, which has been shown to be due, in part, to the action of the envelope protein p15E. We studied a synthetic peptide (CKS-17) homologous to a highly conserved domain of the retroviral envelope protein p15E, which, when conjugated to BSA (CKS-17-BSA), can inhibit IL-1- and phorbol ester-mediated responses in cultured murine thymoma cells, and Ca2(+)- and phosphatidylserine-dependent protein kinase C (PKC) activity of cell homogenates. We characterized the mechanism of inhibition of PKC by the peptide. Using PKC purified from rat

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Alhazmi, Hassan A., Waquar Ahsan, Angum M. M. Ibrahim, et al. "Investigation of bovine serum albumin aggregation upon exposure to silver(i) and copper(ii) metal ions using Zetasizer." Open Chemistry 19, no. 1 (2021): 987–97. http://dx.doi.org/10.1515/chem-2021-0089.

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Abstract Depending upon the metal coordination capacity and the binding sites of proteins, interaction between metal and proteins leads to a number of changes in the protein molecule which may include the change in conformation, unfolding, overall charge, and aggregation in some cases. In this study, Cu(ii) and Ag(i) metal ions were selected to investigate aggregation of bovine serum albumin (BSA) molecule upon interaction by measuring the size and charge of the aggregates using nano-Zetasizer instrument. Two concentrations of metal ions were made to interact with a specific concentration of B

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McKay, M. K., and V. H. Huxley. "ANP increases capillary permeability to protein independent of perfusate protein composition." American Journal of Physiology-Heart and Circulatory Physiology 268, no. 3 (1995): H1139—H1148. http://dx.doi.org/10.1152/ajpheart.1995.268.3.h1139.

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In further studying hormonal regulation of microvascular exchange, we tested two hypotheses: 1) atrial natriuretic peptide (ANP) would increase capillary permeability to protein and 2) the actions of ANP on capillary permeability would be independent of the perfusate protein composition. These hypotheses were tested by assessing in situ capillary transport coefficients: hydraulic conductivity (Lp), apparent and diffusive permeabilities (Ps and Psd, respectively) to the protein alpha-lactalbumine, and convective coupling [Lp(1-sigma)] in mesenteric capillaries in cerebrally pithed frogs (Rana p

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Kovalska, Vladyslava, Marina Kuperman, Mykhaylo Losytskyy, et al. "Induced CD of iron(ii) clathrochelates: sensing of the structural and conformational alterations of serum albumins." Metallomics 11, no. 2 (2019): 338–48. http://dx.doi.org/10.1039/c8mt00278a.

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