Academic literature on the topic 'Bullfrog'

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Journal articles on the topic "Bullfrog"

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Flynn, Lauren, Tess Kreofsky, and Adam Sepulveda. "Introduced American Bullfrog Distribution and Diets in Grand Teton National Park." UW National Parks Service Research Station Annual Reports 38 (January 1, 2015): 17–28. http://dx.doi.org/10.13001/uwnpsrc.2015.4081.

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Introduced American Bullfrogs (Lithobates catesbeianus) have been present in Grand Teton National Park since approximately the 1950s, but little is known about their distribution and potential impacts. In this study, we surveyed the current bullfrog distribution and spatial overlap with sympatric native amphibians in the park, and characterized post-metamorphic bullfrog diets from July – September 2015. Despite surveys in multiple large rivers and floodplain habitats, we only documented bullfrogs in a geothermal pond and 5 km of stream channel immediately downstream of this pond. In these waters, bullfrogs overlapped with native amphibians at the downstream end of their distribution, and we did not document native amphibians in bullfrog stomach contents. Larger bullfrogs (SVL ≥ 96 mm) primarily consumed native rodents (especially meadow voles, Microtus pennsylvanicus), while smaller bullfrogs frequently consumed native invertebrates and less frequently consumed non-native invertebrates and fish. Taken together, these data indicate that the distribution and implications of the bullfrog invasion in Grand Teton National Park are currently localized to a small area, so these bullfrogs should therefore be vulnerable to eradication.
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Kamoroff, Colleen, Ninette Daniele, Robert L. Grasso, Rebecca Rising, Travis Espinoza, and Caren S. Goldberg. "Effective removal of the American bullfrog (Lithobates catesbeianus) on a landscape level: long term monitoring and removal efforts in Yosemite Valley, Yosemite National Park." Biological Invasions 22, no. 2 (October 22, 2019): 617–26. http://dx.doi.org/10.1007/s10530-019-02116-4.

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Abstract Invasive alien species are a major threat to freshwater ecosystems, and American bullfrogs are among the world’s 100 most prominent aquatic invasive species causing negative direct and indirect effect on native aquatic fauna worldwide. Bullfrogs were intentionally introduced into Yosemite Valley, Yosemite National Park in the 1950s where they became well established in the subsequent years. Starting in 2005, the National Park Service (NPS) began bullfrog removal, targeting various life stages using hand, net, and spear techniques. Starting in 2015, the NPS conducted environmental DNA (eDNA) surveys and deployed audio recordings devices to ensure adequate detection of bullfrogs. During the first year of cencerted effort in the Valley in 2005, the NPS removed 86% of all recorded bullfrog. The subsequent decade was spent searching for individuals with lower return on effort. In 2012, the NPS removed the last observed signs of bullfrog breeding, and the last observed bullfrog in 2019. Following removal of the breeding bullfrog population, the NPS began restoration projects for species of special concern. The NPS introduced the federally threatened California red-legged frogs (Rana draytonii) into Yosemite Valley beginning in 2016. This is the first published successful eradication of bullfrogs on a landscape level. National Parks and Monuments often provide refuges for imperiled wildlife and should be managed to remove invasive species. Our work highlights effective bullfrog removal is obtainable and can lead to local recovery of endangered species.
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Boelter, Ruben A., Igor L. Kaefer, Camila Both, and Sonia Cechin. "Invasive bullfrogs as predators in a Neotropical assemblage: What frog species do they eat?" Animal Biology 62, no. 4 (2012): 397–408. http://dx.doi.org/10.1163/157075612x634111.

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Anurans are important prey for the American bullfrog Lithobates catesbeianus, but field assessments of its diet in the context of a local prey assemblage are lacking. We aimed to identify the frog species consumed by an invasive bullfrog population in subtropical South America, and to assess their relative importance among other types of prey. Characterization of the frog assemblage in the study area also allowed us to calculate the degree of electivity of the recorded anuran prey, in order to gain insight regarding bullfrog feeding preferences and to test if the bullfrog prey composition differed from a random sample of the assemblage. A total of 32.6% of the bullfrogs had at least one anuran in the stomach contents, and post-metamorphic anurans represented 49.1% of the relative prey importance for adult bullfrogs. Anurans were preyed on by all size classes, and constituted the volumetrically most important prey category in the diet of individuals heavier than 100 g. Cycloramphidae, Hylidae and Leiuperidae were positively selected, and Hypsiboas pulchellus and Physalaemus cuvieri were the species most often taken. We found a low occurrence of cannibalism, despite the high density of bullfrogs at the study site. Our results showed that the degree of electivity differed among bullfrog prey types, suggesting that some frog species may be preyed on in a higher proportion than their relative abundance in the assemblage. Testing the clues provided by this assemblage-level approach may lead to a better assessment of the interactions between bullfrogs and the native frog fauna.
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Sugiura, Shinji, and Tomoki Date. "Bombardier beetles repel invasive bullfrogs." PeerJ 10 (September 15, 2022): e13805. http://dx.doi.org/10.7717/peerj.13805.

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Invasive non-native predators negatively affect native species; however, some native species can survive the predation pressures of invasive species by using pre-existing antipredator strategies or evolving defenses against invasive predators. The American bullfrog Lithobates catesbeianus (Anura: Ranidae) has been intentionally introduced to many countries and regions, and has impacted native animals through direct predation. Bombardier beetles (Coleoptera: Carabidae: Brachininae: Brachinini) discharge chemicals at a temperature of approximately 100 °C from the tip of the abdomen when they are attacked by predators. This “bombing” can successfully repel predators. However, adults of a native bombardier beetle Pheropsophus (Stenaptinus) occipitalis jessoensis have been reportedly found in the gut contents of the introduced bullfrog L. catesbeianus in Japan. These records suggest that the invasive bullfrog L. catesbeianus attacks the native bombardier beetle P. occipitalis jessoensis under field conditions in Japan; however, the effectiveness of the bombing defense against invasive bullfrogs is unclear. To test the effectiveness of the bombing defense against bullfrogs, we investigated the behavioral responses of L. catesbeianus juveniles to P. occipitalis jessoensis adults under laboratory conditions. Contrary to previous gut content results, almost all the bullfrogs (96.3%) rejected bombardier beetles before swallowing them; 88.9% rejected the beetles after being bombed, and 7.4% stopped attacking the beetles before being bombed. Only 3.7% successfully swallowed and digested the beetle. All of the beetles collected from non-bullfrog-invaded sites could deter bullfrogs, suggesting that the pre-existing defenses of bombardier beetles played an essential role in repelling bullfrogs. When treated beetles that were unable to discharge hot chemicals were provided, 77.8% of bullfrogs successfully swallowed and digested the treated beetles. These results indicate that bombing is important for the successful defense of P. occipitalis jessoensis against invasive bullfrogs. Although invasive bullfrogs have reportedly impacted native insect species, P. occipitalis jessoensis has an existing defense mechanism strong enough to repel the invasive predators.
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Courtois, Daniel, Raymond Leclair jr., Sylvain Lacasse, and Pierre Magnan. "Habitats préférentiels d'amphibiens ranidés dans des lacs oligotrophes du Bouclier laurentien, Québec." Canadian Journal of Zoology 73, no. 9 (September 1, 1995): 1744–53. http://dx.doi.org/10.1139/z95-206.

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From a study of riparian habitat structure and a quantitive distribution survey of bullfrog, Rana catesbeiana, mink frog, Rana septentrionalis, and green frog, Rana clamitans melanota, in 31 oligotrophic lakes, we looked for, among 18 physiographic parameters, those that could best explain the spatial organisation of the ranid community. The three species cohabitated in 18 lakes, the mink frog and the green frog in 10 lakes without bullfrog, and the bullfrog alone in 3 lakes. These frogs preferentially occupied (i) habitats with medium or high density of emergent vegetation, (ii) areas with extensive floating aquatic vegetation, (iii) muddy and silty areas, and (iv) especially for the green frog, shrubby habitats with ericaceae. Substrates had a poor explicative value. In lakes devoid of bullfrogs, the mink frogs and green frogs were more frequently abundant and showed a more even distribution in the different habitats than when they were sympatric with bullfrogs. A Spearman's rank correlation analysis confirmed the similarity of habitat preferences between the three species and the poor capacity of the habitat structure to predict the ranid community composition.
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Tasker, Bailey R., Karli N. Honebein, Allie M. Erickson, Julia E. Misslin, Paul Hurst, Sarah Cooney, Skylar Riley, Scott A. Griffith, and Betsy A. Bancroft. "Effects of elevated temperature, reduced hydroperiod, and invasive bullfrog larvae on pacific chorus frog larvae." PLOS ONE 17, no. 3 (March 15, 2022): e0265345. http://dx.doi.org/10.1371/journal.pone.0265345.

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Climate change and invasive species threaten many ecosystems, including surface freshwater systems. Increasing temperatures and reduced hydroperiod due to climate change may promote the persistence of invasive species and facilitate new invasions due to potentially higher tolerance to environmental stress in successful invaders. Amphibians demonstrate high levels of plasticity in life history characteristics, particularly those species which inhabit both ephemeral and permanent water bodies. We tested the influence of two projected effects of climate change (increased temperature and reduced hydroperiod) on Pacific chorus frog (Pseudacris regilla) tadpoles alone and in combination with the presence of tadpoles of a wide-spread invasive amphibian, the American bullfrog (Lithobates catesbeianus). Specifically, we explored the effects of projected climate change and invasion on survival, growth, mass at stage 42, and development rate of Pacific chorus frogs. Direct and indirect interactions between the invasive tadpole and the native tadpole were controlled via a cage treatment and were included to account for differences in presence of the bullfrog compared to competition for food resources and other direct effects. Overall, bullfrogs had larger negative effects on Pacific chorus frogs than climate conditions. Under future climate conditions, Pacific chorus frogs developed faster and emerged heavier. Pacific chorus frog tadpoles developing in the presence of American bullfrogs, regardless of cage treatment, emerged lighter. When future climate conditions and presence of invasive American bullfrog tadpoles were combined, tadpoles grew less. However, no interaction was detected between climate conditions and bullfrog presence for mass, suggesting that tadpoles allocated energy towards mass rather than length under the combined stress treatment. The maintenance of overall body condition (smaller but heavier metamorphs) when future climate conditions overlap with bullfrog presence suggests that Pacific chorus frogs may be partially compensating for the negative effects of bullfrogs via increased allocation of energy towards mass. Strong plasticity, as demonstrated by Pacific chorus frog larvae in our study, may allow species to match the demands of new environments, including under future climate change.
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Li, Yiming, Zhunwei Ke, Yihua Wang, and Tim M. Blackburn. "Frog community responses to recent American bullfrog invasions." Current Zoology 57, no. 1 (February 1, 2011): 83–92. http://dx.doi.org/10.1093/czoolo/57.1.83.

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Abstract Native species may decline quickly when confronted with an exotic species to which they are not adapted. The extent of decline may depend on the abundance of an invader and the length of time since it first arrived in the community (residence time), and the interaction between these two variables. We tested these effects using data on the effects of American bullfrog Lithobates catesbeianus invasion on native frog communities in 65 permanent lentic waters on islands in the Zhoushan Archipelago, China. We examined variation in native frog abundance and species richness in relation to features of the American bullfrog invasion, habitat disturbance, characteristics of the water body and fish communities and the presence of red swamp crayfish. Bullfrog invaded sites had lower native frog density and species richness, higher submerged vegetation cover and greater frequency of repairs to the water body than did non-invaded sites. The minimum adequate general linear mixed models showed that both native frog density and species richness were negatively related to post-metamorphosis bullfrog density, and that native frog species richness was also positively related to the vegetation cover. There was no effect on either native frog density or species richness of residence time or its interaction with bullfrog density, or of the abundance of bullfrog tadpoles. The results suggested that post-metamorphosis bullfrogs had impacts on native frog communities in the islands, and that the extents of these impacts are proportional to post-metamorphosis bullfrog density.
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MEDEIROS, CAMILA I., CAMILA BOTH, IGOR L. KAEFER, and SONIA Z. CECHIN. "Reproductive phenology of the American Bullfrog in subtropical Brazil: photoperiod as a main determinant of seasonal activity." Anais da Academia Brasileira de Ciências 88, no. 3 suppl (July 11, 2016): 1909–21. http://dx.doi.org/10.1590/0001-3765201620150694.

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ABSTRACT The North American bullfrog Lithobates catesbeianus continues to invade ecosystems worldwide, potentially causing population declines and even extinctions. Within its native distribution, bullfrogs show prolonged reproductive seasons and high fertility. However, data on breeding biology of bullfrogs ex-situ in invaded localities mainly comes from anecdotal reports. Understanding how invasive species are adjusting their life histories to new colonized environments is important for conservation purposes. Here we describe temporal and spatial abundance, calling activity, spawning and tadpole distribution of bullfrogs in southern Brazil. Eighteen samplings occurred during one year. The abundance of individuals was positively related to longer photoperiods and higher temperatures. Reproductive activity was also positively associated with longer photoperiods. Calling sites, spawning and tadpoles were associated with microhabitats presenting hydrophytes, which may provide shelter and thermal stability to bullfrogs. The reproductive seasonal activity of bullfrogs can be highly variable across its growing geographical range, but in subtropical Brazil it is associated with photoperiod, a highly predictable abiotic determinant. In our study area, bullfrogs presented a breeding season twice as long as that observed in some native localities. We suggest that management strategies directed to bullfrog populations must consider the habitat structures and seasonal regimes determined by each invaded environment.
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Li, Fulong, Baipeng Chen, Ming Xu, Yang Feng, Yongqiang Deng, Xiaoli Huang, Yi Geng, Ping Ouyang, and Defang Chen. "Immune Activation and Inflammatory Response Mediated by the NOD/Toll-like Receptor Signaling Pathway—The Potential Mechanism of Bullfrog (Lithobates catesbeiana) Meningitis Caused by Elizabethkingia miricola." International Journal of Molecular Sciences 24, no. 19 (September 26, 2023): 14554. http://dx.doi.org/10.3390/ijms241914554.

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Elizabethkingia miricola is an emerging opportunistic pathogen that is highly pathogenic in both immunocompromised humans and animals. Once the disease occurs, treatment can be very difficult. Therefore, a deep understanding of the pathological mechanism of Elizabethkingia miricola is the key to the prevention and control of the disease. In this study, we isolated the pathogenic bacteria from bullfrogs with dark skin color, weak limbs, wryneck, and cataracts. Via subsequent morphological observations and a 16S rRNA gene sequence analysis, the pathogen was identified as Elizabethkingia miricola. The histopathological and transmission electron microscopy analysis revealed that the brain was the main target organ. Therefore, brain samples from diseased and healthy bullfrogs were used for the RNA-Seq analysis. The comparative transcriptome analysis revealed that the diseased bullfrog brain was characterized by the immune activation and inflammatory response, which were mediated by the “NOD-like receptor signaling pathway” and the “Toll-like receptor signaling pathway”. We also performed qRT-PCR to examine the expression profile of inflammation-related genes, which further verified the reliability of our transcriptome data. Based on the above results, it was concluded that the NOD/Toll-like receptor-related networks that dominate the immune activation and inflammatory response were activated in the brain of Elizabethkingia miricola-infected bullfrogs. This study contributes to the search for therapeutic targets for bullfrog meningitis and provides basic information for establishing effective measures to prevent and control bullfrog meningitis.
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Takahashi, N., S. Kikuyama, K. Gen, O. Maruyama, and Y. Kato. "Cloning of a bullfrog growth hormone cDNA: expression of growth hormone mRNA in larval and adult bullfrog pituitaries." Journal of Molecular Endocrinology 9, no. 3 (December 1992): 283–89. http://dx.doi.org/10.1677/jme.0.0090283.

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ABSTRACT A GH cDNA was specifically amplified from cDNAs constructed from total RNA of bullfrog (Rana catesbeiana) adenohypophyses employing the DNA polymerase chain reaction. Sequencing analysis revealed that the cDNA clone thus obtained was 654 bp in length, and included an open reading frame encoding the entire sequence of mature GH, with its signal peptide. Slight discrepancies were noted between the deduced amino acid sequence and that determined by direct protein sequencing of purified bullfrog GH or that deduced from the nucleotide sequence reported previously. The length of the bullfrog GH mRNA was estimated to be about 1·2 kb by Northern blot analysis. Homologies of nucleotide and amino acid sequences between GH and prolactin of bullfrog origin were 48% and 26% respectively. Using the cDNA as a probe, the content of GH mRNA in the pituitary of larval and adult bullfrogs was measured. GH mRNA levels were relatively low at the preclimax stage, and rose markedly during climax. In juvenile frogs, GH mRNA levels in the pituitary were extremely high and declined towards adulthood. This finding suggests that the increase in plasma and pituitary GH levels reported previously accompanies the increase in GH synthesis.
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Dissertations / Theses on the topic "Bullfrog"

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Petersen, Ann Marie. "Metabolic plasticity in the American bullfrog (Lithobates catesbeiana)." Connect to online resource, 2008. http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:dissertation&res_dat=xri:pqdiss&rft_dat=xri:pqdiss:3303857.

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Bee, Mark Allen. "Vocally mediated neighbor recognition in North American bullfrogs, Rana catesbeiana : identification, perception, and learning /." free to MU campus, to others for purchase, 2001. http://wwwlib.umi.com/cr/mo/fullcit?p3012947.

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Yetman, Caroline Angela. "Conservation biology of the giant bullfrog, Pyxicephalus adspersus (Tschudi, 1838)." Thesis, University of Pretoria, 2012. http://hdl.handle.net/2263/31245.

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The giant bullfrog, Pyxicephalus adspersus, is a large, explosive-breeding anuran from southern Africa, which spends most of the year buried in a state of torpor. In South Africa this species is considered to be Near-Threatened by habitat loss and other factors, especially in the densely human populated Gauteng Province. The aim of this thesis was to obtain essential outstanding information about the ecology of P. adspersus to contribute towards improved conservation management of this species. A model was used to predict the geographic range of P. adspersus in southern Africa, and recent land cover data were used to determine the amount of suitable habitat remaining for this species in Gauteng. As a step towards identifying P. adspersus conservation management units, genetic structure and gene flow for populations from 23 localities in Gauteng and seven additional localities in the north-eastern interior of South Africa was quantified using 708 base pairs of the mitochondrial gene cytochrome b. To investigate the unpredictable activity and unknown spatial habitat requirements of P. adspersus, a population’s spawning and non-breeding activity was monitored, and the movements of 70 adult frogs were radio- or spool-tracked during five summers at a site in Diepsloot, Gauteng. Using skeletohronology, the age distribution of breeding P. adspersus at this and two other peri-urban sites near Johannesburg, Gauteng, was examined. Bioclimatic conditions were predicted to be suitable for P. adspersus in the temperate to semi-arid interior, but not the low-lying eastern subtropical and arid western sides of southern Africa. Limited genetic data suggested that P. adspersus was common in the north-eastern interior of South Africa, and that populations in the Free State Province represent an evolutionary significant unit of this species. In central Gauteng, where P. adspersus may have declined by > 90%, populations < 20 km apart exhibited significant genetic differentiation, possibly as a result of genetic drift. At Diepsloot, both annual numbers of spawning events and numbers of spawning males were positively correlated with rainfall, although other meteorological variables also affected the activity of P. adspersus. Radio- or spool-tracked frogs showed high fidelity to their breeding site and burrows, which were situated up to 1 km away from the water. Male P. adspersus probably live 20 years in the wild, but at some peri-urban breeding sites adult life expectancy and body size may be declining. The geographic range of P. adspersus was predicted to be slightly smaller than that reported by other authors, and deserves phylogeographic validation. The main conservation priority for P. adspersus in South Africa should be the protection of terrestrial habitat for adult foraging and aestivation around, and for juvenile dispersal and gene flow between, breeding sites. In Gauteng, the conservation of a P. adspersus metapopulation is critical, and could most likely be achieved in the northern region of this province. Populations in the Free State Province deserve improved protection given their reported genetic uniqueness. At local spatial scales specific threats (e.g. pollution) should be ameliorated, and long-term monitoring should be implemented to detect real population trends.
Thesis (PhD)--University of Pretoria, 2012.
Zoology and Entomology
Unrestricted
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Adams, Michael J. "Experimental analysis of tadpole mortality factors : effects of bullfrogs and exotic fish in the Puget Lowlands, Washington /." Thesis, Connect to this title online; UW restricted, 1997. http://hdl.handle.net/1773/5507.

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Connell, Michael W. (Michael Wayne). "Bullfrog--an extensible, modular toolkit for the construction of NuMesh applications." Thesis, Massachusetts Institute of Technology, 1996. http://hdl.handle.net/1721.1/41787.

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Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1996.
Includes bibliographical references (leaves 82-83).
by Michael W. Connell.
M.S.
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Lowes, Vicki L. "Molecular and biophysical analysis of the delayed rectifier K+ current in bullfrog atrium." Thesis, National Library of Canada = Bibliothèque nationale du Canada, 1998. http://www.collectionscanada.ca/obj/s4/f2/dsk2/ftp02/NQ34684.pdf.

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Emery, David. "ASSESSING THE GENOTOXICITY OF TRICLOSAN IN TADPOLES OF THE AMERICAN BULLFROG, LITHOBATES CATESBEIANUS." VCU Scholars Compass, 2012. http://scholarscompass.vcu.edu/etd/345.

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Amphibians are particularly sensitive to environmental degradation and, therefore, serve as effective environmental quality indicators. Research has suggested that amphibian declines are exacerbated by manmade environmental toxicants, especially those found in high concentrations in urban areas. The NIH has pinpointed genotoxicity as a major route of cancer causation, and has since developed stringent testing procedures for potentially hazardous chemicals. One such method, recognized for its simplicity and economy, is the micronucleus assay. A study was conducted assessing the genotoxicity of the widely used antimicrobial agent Triclosan to American Bullfrog tadpoles. Lithobates catesbeianus tadpoles were reared in glass aquaria containing ultra-high purity water and were dosed with nominal concentrations of 2.3 µg/L, 23 µg/L, and 230 µg/L Triclosan, reflecting 1x, 10x, and 100x concentrations of the compound as found in US surface waters. Eight replicates of each of the three levels of Triclosan contamination were prepared, as well as eight replicates per control group. Each replicate contained three tadpoles in a glass aquarium, from which one tadpole per tank was sampled after 1, 8, or 15 days following initial exposure to test compounds. Erythrocytes were prepared on slides and scored for micronucleus presence under 1000x magnification. Triclosan induced significant micronucleus formation after only 24 hours in all treatments relative to the negative control and exhibited a maximum of 15 micronuclei per 2,000 erythrocytes scored. Modeling of MN induction dynamics by treatment suggested that the best predictor of micronucleus induction was the acute TCS exposure level, as described by a linear mixed effects model including a binomial term of time exposed. Micronucleus induction was TCS concentration dose-dependent. This study supports that Triclosan induces significant genetic damage at environmentally relevant concentrations. It is clear that the effects of genotoxic agents must be certified so proper regulatory protocols can be developed and enforced, in order to conserve wildlife and promote human health.
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Colósio, Rafael Rodrigues [UNESP]. "Atividade de enzimas relacionadas ao processo de ossificação em girinos de Lithobates catesbeianus." Universidade Estadual Paulista (UNESP), 2015. http://hdl.handle.net/11449/126536.

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A calcificação biológica é um processo muito bem regulado, no qual os diferentes tipos de tecidos, células, organelas e biomoléculas, participam na coordenação e regulação de eventos metabólicos envolvidos na deposição de fosfato de cálcio, sob a forma de cristais de hidroxiapatita. As alterações morfológicas ocorridas nos anuros, durante a metamorfose, são extremamente acentuadas e perceptíveis, sendo uma delas a remodelação do esqueleto. Os eventos relacionados à ossificação em girinos raramente são descritos na literatura. A fosfatase ácida tartarato resistente tem sido amplamente utilizada como um marcador específico de osteoclastos, células que participam do processo de reabsorção e de remodelação do tecido ósseo, enquanto a fosfatase alcalina tem sido usada como marcador de osteoblastos, células responsáveis pela formação do tecido ósseo. Estudos efetuados por vários pesquisadores, com o objetivo de determinar, principalmente, as enzimas presentes nas vesículas extracelulares dos condrócitos, têm revelado a presença de outras enzimas, além da fosfatase alcalina que são importantes para o processo de calcificação biológica. Assim, no presente trabalho foi avaliada a variação na atividade das fosfatases no processo de ossificação no período de desenvolvimento dos membros de Lithobates catesbeianus, a fim de contribuir na compreensão deste processo não somente em anuros, mas também nos demais vertebrados. Os animais foram dessensibilizados em água com gelo, decapitados, os membros foram removidos, em seguida os ossos foram descarnados e homogeneizados, centrifugados, sendo o sobrenadante aliquotado, congelado em nitrogênio líquido e armazenado a -70ºC para posteriores atividades enzimáticas e dosagem de proteína no extrato. As enzimas, fosfatase ácida e fosfatase alcalina, apresentaram estabilidade em todos os pH de armazenamento estudados...
Biological calcification is a tight regulated process in which different types of tissues, cells, organelles, and biomolecules participate in the coordination and regulation of metabolic events involved in accumulating calcium phosphate, in the form of hidroxiapatite crystals. The morphological changes that occur during anuran metamorphosis are extremely accentuated and perceptible, such as the remodeling of the skeleton. The ossification events are rarely described for tadpoles in the literature. The tartarate resistant acid phosphatase has been widely used as a specific marker of osteoclasts, cells that participate in the process of resorption and remodeling of bone tissue, while the alkaline phosphatase has been used as a marker for osteoblasts, cells responsible for bone tissue formation. Studies conducted by many researchers with the aim of determining, mainly, the enzymes in chondrocyte extracellular vesicles have revealed the presence of other enzymes, in addition to alkaline phosphatase, which are important to the process of biological calcification. Thus, in the present study, the changes in the activity of phosphatases in the ossification process during the development of the limbs of Lithobates catesbeianus was evaluated, with the aim to contribute to the understanding of this process not only in anurans, but also in other vertebrates. The animals were desensitized in water with ice, decapitated and limb bones were removed and homogenized, centrifuged, and the supernatant aliquoted, frozen in liquid nitrogen and stored at -70ºC for subsequent enzymatic activities and protein quantification. The enzymes, acid phosphatase and alkaline phosphatase, remained stable in all of the studied storage pH, the apparent pH optimum of hydrolysis of p-nitrophenyl phosphate (PNPP) was of 5.0 and 10.5, respectively, and the enzymes were stable at 45ºC and the t1/2 was 60 minutes at 55ºC for alkaline...
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Colósio, Rafael Rodrigues. "Atividade de enzimas relacionadas ao processo de ossificação em girinos de Lithobates catesbeianus /." Araraquara, 2015. http://hdl.handle.net/11449/126536.

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Orientador: João Martins Pizauro Junior
Banca: Marta Verardino de Stéfani
Banca: Luis Henrique Souza Guimarães
Resumo: A calcificação biológica é um processo muito bem regulado, no qual os diferentes tipos de tecidos, células, organelas e biomoléculas, participam na coordenação e regulação de eventos metabólicos envolvidos na deposição de fosfato de cálcio, sob a forma de cristais de hidroxiapatita. As alterações morfológicas ocorridas nos anuros, durante a metamorfose, são extremamente acentuadas e perceptíveis, sendo uma delas a remodelação do esqueleto. Os eventos relacionados à ossificação em girinos raramente são descritos na literatura. A fosfatase ácida tartarato resistente tem sido amplamente utilizada como um marcador específico de osteoclastos, células que participam do processo de reabsorção e de remodelação do tecido ósseo, enquanto a fosfatase alcalina tem sido usada como marcador de osteoblastos, células responsáveis pela formação do tecido ósseo. Estudos efetuados por vários pesquisadores, com o objetivo de determinar, principalmente, as enzimas presentes nas vesículas extracelulares dos condrócitos, têm revelado a presença de outras enzimas, além da fosfatase alcalina que são importantes para o processo de calcificação biológica. Assim, no presente trabalho foi avaliada a variação na atividade das fosfatases no processo de ossificação no período de desenvolvimento dos membros de Lithobates catesbeianus, a fim de contribuir na compreensão deste processo não somente em anuros, mas também nos demais vertebrados. Os animais foram dessensibilizados em água com gelo, decapitados, os membros foram removidos, em seguida os ossos foram descarnados e homogeneizados, centrifugados, sendo o sobrenadante aliquotado, congelado em nitrogênio líquido e armazenado a -70ºC para posteriores atividades enzimáticas e dosagem de proteína no extrato. As enzimas, fosfatase ácida e fosfatase alcalina, apresentaram estabilidade em todos os pH de armazenamento estudados...
Abstract: Biological calcification is a tight regulated process in which different types of tissues, cells, organelles, and biomolecules participate in the coordination and regulation of metabolic events involved in accumulating calcium phosphate, in the form of hidroxiapatite crystals. The morphological changes that occur during anuran metamorphosis are extremely accentuated and perceptible, such as the remodeling of the skeleton. The ossification events are rarely described for tadpoles in the literature. The tartarate resistant acid phosphatase has been widely used as a specific marker of osteoclasts, cells that participate in the process of resorption and remodeling of bone tissue, while the alkaline phosphatase has been used as a marker for osteoblasts, cells responsible for bone tissue formation. Studies conducted by many researchers with the aim of determining, mainly, the enzymes in chondrocyte extracellular vesicles have revealed the presence of other enzymes, in addition to alkaline phosphatase, which are important to the process of biological calcification. Thus, in the present study, the changes in the activity of phosphatases in the ossification process during the development of the limbs of Lithobates catesbeianus was evaluated, with the aim to contribute to the understanding of this process not only in anurans, but also in other vertebrates. The animals were desensitized in water with ice, decapitated and limb bones were removed and homogenized, centrifuged, and the supernatant aliquoted, frozen in liquid nitrogen and stored at -70ºC for subsequent enzymatic activities and protein quantification. The enzymes, acid phosphatase and alkaline phosphatase, remained stable in all of the studied storage pH, the apparent pH optimum of hydrolysis of p-nitrophenyl phosphate (PNPP) was of 5.0 and 10.5, respectively, and the enzymes were stable at 45ºC and the t1/2 was 60 minutes at 55ºC for alkaline...
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Rodriquez-Contreras, Adrian. "PERMEATION AND GATING PROPERTIES OF PRESYNAPTIC CALCIUM CHANNELS IN HAIR CELLS OF RANA CATESBEIANA." University of Cincinnati / OhioLINK, 2001. http://rave.ohiolink.edu/etdc/view?acc_num=ucin991400738.

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Books on the topic "Bullfrog"

1

Gray, Susan Heinrichs. Bullfrog. Ann Arbor, Mich: Cherry Lake Pub., 2009.

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Rustad, Martha E. H. Bullfrogs. Minneapolis, Minn: Jump!, 2014.

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illustrator, McAllister Chris 1958, ed. Bullfrog pops! New York, N.Y: Scholastic Inc., 2000.

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Hausman, Sidney. One bullfrog. Santa Fe, N.M: Azro Press, 2002.

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Walton, Rick. Bullfrog pops! Salt Lake City: Gibbs Smith, 1999.

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Hu, Qian. Niu wa: Bullfrog. Beijing Shi: Jiu zhou chu ban she, 2017.

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West, Colin. "Hello, great big bullfrog!". New York: J.B. Lippincott, 1987.

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West, Colin. "Hello, great big bullfrog". London: Walker, 1987.

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Dennard, Deborah. Bullfrog at Magnolia Circle. Norwalk, Conn: Soundprints, 2002.

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Oldfield, Dawn Bluemel. Water frog polliwogs. New York, N.Y: Bearport Pub., 2013.

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Book chapters on the topic "Bullfrog"

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de Moraes, D. N., D. IKozusny-Andreani, C. R. Tim, L. Assis, A. P. Da Costa, and M. M. Amaral. "Study on the Disinfection Stability of Bullfrog Skin." In XXVII Brazilian Congress on Biomedical Engineering, 173–77. Cham: Springer International Publishing, 2022. http://dx.doi.org/10.1007/978-3-030-70601-2_28.

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Adams, Paul R., David A. Brown, Andrew Constanti, Robert B. Clark, and Leslie Satin. "Calcium-Activated Potassium Channels in Bullfrog Sympathetic Ganglion Cells." In Calcium in Biological Systems, 181–91. Boston, MA: Springer US, 1985. http://dx.doi.org/10.1007/978-1-4613-2377-8_21.

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Fujiyama, Rie, Takenori Miyamoto, and Toshihide Sato. "Distribution of Ion Channels on the Bullfrog Taste Cell Membrane." In Olfaction and Taste XI, 109. Tokyo: Springer Japan, 1994. http://dx.doi.org/10.1007/978-4-431-68355-1_44.

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Hyodo, S. "Expression of vasotocin gene during metamorphosis in the bullfrog hypothalamus." In The Peptidergic Neuron, 251–57. Basel: Birkhäuser Basel, 1996. http://dx.doi.org/10.1007/978-3-0348-9010-6_27.

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Tatsuta, Takeo, and Masahiro Hosono. "Effects of Bullfrog Sialic Acid–Binding Lectin in Cancer Cells." In Glycosignals in Cancer, 125–47. Singapore: Springer Nature Singapore, 2023. http://dx.doi.org/10.1007/978-981-19-7732-9_7.

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Peng, Yan-yi. "Dynamics Of Presynaptic Ca2+ And Peptide Release In Bullfrog Sympathetic Ganglia." In Computation in Neurons and Neural Systems, 39–44. Boston, MA: Springer US, 1994. http://dx.doi.org/10.1007/978-1-4615-2714-5_7.

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Minota, S. "Modulation of Cholinergic Synaptic Transmission by Arachidonic Acid in Bullfrog sympathetic Neurons." In Slow Synaptic Responses and Modulation, 425–28. Tokyo: Springer Japan, 2000. http://dx.doi.org/10.1007/978-4-431-66973-9_58.

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Li, Yao, Hai-Qing Gong, Pei-Ji Liang, and Pu-Ming Zhang. "The Spatiotemporal Structure of Receptive Field of Ganglion Cells in Bullfrog Retina." In Advances in Cognitive Neurodynamics (II), 207–11. Dordrecht: Springer Netherlands, 2010. http://dx.doi.org/10.1007/978-90-481-9695-1_32.

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Fujita, K., H. Tokuno, and K. Kuba. "Temporarily Distinct Induction of Two Phases of Long-Term Potentiation in Bullfrog Sympathetic Ganglia." In Slow Synaptic Responses and Modulation, 320–22. Tokyo: Springer Japan, 2000. http://dx.doi.org/10.1007/978-4-431-66973-9_41.

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Nakamura, Mikihiko, Kaoru Tsujii, and Junzo Sunamoto. "Direct Extraction of Taste Receptor Proteins by Liposome from Intact Epithelium of Bullfrog Tongue." In Advanced Biomaterials in Biomedical Engineering and Drug Delivery Systems, 271–72. Tokyo: Springer Japan, 1996. http://dx.doi.org/10.1007/978-4-431-65883-2_72.

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Conference papers on the topic "Bullfrog"

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Bhattacherjee, Souvik, Gang Liao, Michael Hicks, and Daniel J. Abadi. "BullFrog: Online Schema Evolution via Lazy Evaluation." In SIGMOD/PODS '21: International Conference on Management of Data. New York, NY, USA: ACM, 2021. http://dx.doi.org/10.1145/3448016.3452842.

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Meenderink, Sebastiaan W. F., and Dolores Bozovic. "Motion of the otolithic membrane in the bullfrog sacculus." In MECHANICS OF HEARING: PROTEIN TO PERCEPTION: Proceedings of the 12th International Workshop on the Mechanics of Hearing. AIP Publishing LLC, 2015. http://dx.doi.org/10.1063/1.4939330.

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Luo, Huiyu, Ying Qin, Lei Jiang, Junfang Zhang, Ling Zhu, Guangyi Liu, and Lin Lin. "An Experimental Platform for Neural Communication Based on Bullfrog Sciatic Nerve." In GLOBECOM 2023 - 2023 IEEE Global Communications Conference. IEEE, 2023. http://dx.doi.org/10.1109/globecom54140.2023.10437718.

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Alexander, Erika E., and Andrea M. Simmons. "Anatomical changes in the inner ear of the bullfrog across metamorphic development." In 163rd Meeting Acoustical Society of America/ACOUSTCS 2012 HONG KONG. Acoustical Society of America, 2012. http://dx.doi.org/10.1121/2.0000011.

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PURGUE, A. P., and P. M. NARINS. "MECHANICAL BASIS FOR THE FREQUENCY SELECTIVITY IN THE EAR OF THE BULLFROG (RANA CATESBEIANA)." In Proceedings of the International Symposium. WORLD SCIENTIFIC, 2000. http://dx.doi.org/10.1142/9789812793980_0072.

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Roongthumskul, Yuttana, Lea Fredrickson-Hemsing, Albert Kao, Dolores Bozovic, Christopher A. Shera, and Elizabeth S. Olson. "Numerical Study of the Complex Temporal Pattern of Spontaneous Oscillation in Bullfrog Saccular Hair Cells." In WHAT FIRE IS IN MINE EARS: PROGRESS IN AUDITORY BIOMECHANICS: Proceedings of the 11th International Mechanics of Hearing Workshop. AIP, 2011. http://dx.doi.org/10.1063/1.3658071.

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Ma, Xujun, Yiyang Wang, Xiaohu You, Jenshan Lin, and Lianming Li. "Respiratory Pattern Recognition of an Adult Bullfrog Using a 100-GHz CW Doppler Radar Transceiver." In 2019 IEEE MTT-S International Microwave Biomedical Conference (IMBioC). IEEE, 2019. http://dx.doi.org/10.1109/imbioc.2019.8777870.

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Fredrickson, L., A. Cheng, C. E. Strimbu, D. Bozovic, and K. Arisaka. "The use of a CMOS camera to resolve nanometer displacements of hair cell stereocillia in the bullfrog sacculus." In Biomedical Optics (BiOS) 2008, edited by Daniel L. Farkas, Dan V. Nicolau, and Robert C. Leif. SPIE, 2008. http://dx.doi.org/10.1117/12.764186.

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Reports on the topic "Bullfrog"

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Nicholas Garcia, Nicholas Garcia. How do hormones make a bullfrog feel full? Experiment, December 2013. http://dx.doi.org/10.18258/1790.

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Maldonado, F. Geologic map of the northwest quarter of the Bullfrog 15-minute quadrangle, Nye County, Nevada. Office of Scientific and Technical Information (OSTI), December 1990. http://dx.doi.org/10.2172/138117.

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Baker, Tanya G. Mobility of heavy metals in soils and tailings at the Hanover and Bullfrog tailing site, Silver City, New Mexico. New Mexico Bureau of Geology and Mineral Resources, 1993. http://dx.doi.org/10.58799/ofr-393.

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Knudsen, Tyler R., Adam I. Hiscock, William R. Lund, and Steve D. Bowman. Geologic Hazards of the Bullfrog and Wahweap High-Use Areas of Glen Canyon National Recreation Area, San Juan, Kane, and Garfield Counties, Utah, and Coconino County, Arizona. Utah Geological Survey, May 2020. http://dx.doi.org/10.34191/ss-166.

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Tidwell, Kyle. Quantifying the Impacts of a Novel Predator: The Distinctive Case of the Oregon Spotted Frog (Rana pretiosa) and the Invasive American Bullfrog (Rana (Aquarana) catesbeiana). Portland State University Library, January 2000. http://dx.doi.org/10.15760/etd.5388.

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Casper, Gary, Stfani Madau, and Thomas Parr. Acoustic amphibian monitoring, 2019 data summary: Mississippi National River and Recreation Area. National Park Service, December 2022. http://dx.doi.org/10.36967/2295507.

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Abstract:
Amphibians are a Vital Sign indicator for monitoring long-term ecosystem health in seven national park units that comprise the Great Lakes Network. We present here the results for 2019 amphibian monitoring at Mississippi National River and Recreation Area (MISS). Appendices contain tabular summaries for six years of cumulative results. The National Park Service Great Lakes Inventory and Monitoring Network established 10 permanent acoustic amphibian monitoring sites at MISS in 2015. Acoustic samples are collected by placing automated recorders with omnidirectional stereo microphones at each of the 10 sampling sites. Temperature loggers co-located with the recorders also collect air temperature during the sampling period. Eight of the nine species of frog and toad known to occur at MISS were found in 2019. The most well distributed species were Eastern American Toad, Gray Treefrog, Green Frog, and Northern Leopard Frog. Rarer are Blanchard’s Cricket Frog, Cope’s Gray Treefrog, Wood Frog, and Boreal Chorus Frog. American Bullfrog has not yet been detected on GLKN monitored sites but has been recently confirmed nearby (Pigs Eye Lake). Two of the ten sites—MISS02, MISS04—were not sampled in 2019 due to flooding, and occupancy of early calling species at MISS06 was determined inconclusive due to a late sampling start. MISS07 was also deployed late and results may contain some false absences due to late sampling. We expanded analyses and reporting in 2018 to address calling phenology and to provide a second metric for tracking changes in abundance (as opposed to occupancy) across years. Occupancy analyses track whether or not a site was occupied by a species. Abundance is tracked by assessing how the maximum call intensity changes on sites across years, and by how many automated detections are reported from sites across years. Using two independent survey methods, manual and automated, with large sample sizes continues to return reliable results, providing a confident record of site occupancy for most species. There were some data collection issues in 2019, with two ARS units not deployed and two others with late start dates. This did reduce our ability to assess some sites and species. Summaries of 2019 data are shown in Appendices A, B and C, and cumulative data collection result summaries are provided in Appendix E. Since temperature logs show that the threshold of ≥40°F was already exceeded by 1 April in 2019, we recommend a 15 March start date for future data collection.
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Bauder, Javan M., and Chris L. Prewitt. Control of Introduced American Bullfrogs and Support of Chiricahua Leopard Frog Conservation in Southeast Arizona. U.S. Fish and Wildlife Service, July 2024. http://dx.doi.org/10.3996/css82950145.

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Geologic map of the northwest quarter of the Bullfrog 15-minute Quadrangle, Nye County, Nevada. US Geological Survey, 1990. http://dx.doi.org/10.3133/i1985.

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Geologic map of the northeast quarter of the Bullfrog 15-minute Quadrangle, Nye County, Nevada. US Geological Survey, 1990. http://dx.doi.org/10.3133/i2049.

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