Academic literature on the topic 'Burkholderia phytofirmans'
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Journal articles on the topic "Burkholderia phytofirmans"
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Mangmang, Jonathan, Rosalind Deaker, and Gordon Rogers. "Effects of Plant Growth Promoting Rhizobacteria on Seed Germination Characteristics of Tomato and Lettuce." Journal of Tropical Crop Science 1, no. 2 (January 12, 2015): 35–40. http://dx.doi.org/10.29244/jtcs.1.2.35-40.
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Plant growth promoting rhizobacteria (PGPR) represent a wide genera of rhizospheric bacteria which, when introduced in association with the host plant in proper amount, can enhance plant growth and productivity. A series of experiments were conducted to determine the germination responses of tomato and lettuce to PGPR inoculation. Seeds were inoculated with different strains of Azospirillum brasilense Sp7, Sp7-S and Sp245, Herbaspirillum seropedicea and Burkholderia phytofirmans PsJNT. The results reveal that Sp7-S inoculation yielded better germination rate and total germination of tomato. PGPR inoculation, except Sp7, produced longer (28%) and heavier (37%) roots with superior vigor. In lettuce, PGPR strains, except B. phytofirmans PsJNT, and Sp7 and B. phytofirmans PsJNT, enhanced germination vigor and length of roots (26%), respectively. The results provide further evidence concerning their importance as PGPR and indicate the potential of exploiting some of these PGPR to improve seedling emergence and establishment of vegetables.
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Zúñiga, Ana, María Josefina Poupin, Raúl Donoso, Thomas Ledger, Nicolás Guiliani, Rodrigo A. Gutiérrez, and Bernardo González. "Quorum Sensing and Indole-3-Acetic Acid Degradation Play a Role in Colonization and Plant Growth Promotion of Arabidopsis thaliana by Burkholderia phytofirmans PsJN." Molecular Plant-Microbe Interactions® 26, no. 5 (May 2013): 546–53. http://dx.doi.org/10.1094/mpmi-10-12-0241-r.
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Although not fully understood, molecular communication in the rhizosphere plays an important role regulating traits involved in plant–bacteria association. Burkholderia phytofirmans PsJN is a well-known plant-growth-promoting bacterium, which establishes rhizospheric and endophytic colonization in different plants. A competent colonization is essential for plant-growth-promoting effects produced by bacteria. Using appropriate mutant strains of B. phytofirmans, we obtained evidence for the importance of N-acyl homoserine lactone-mediated (quorum sensing) cell-to-cell communication in efficient colonization of Arabidopsis thaliana plants and the establishment of a beneficial interaction. We also observed that bacterial degradation of the auxin indole-3-acetic acid (IAA) plays a key role in plant-growth-promoting traits and is necessary for efficient rhizosphere colonization. Wildtype B. phytofirmans but not the iacC mutant in IAA mineralization is able to restore promotion effects in roots of A. thaliana in the presence of exogenously added IAA, indicating the importance of this trait for promoting primary root length. Using a transgenic A. thaliana line with suppressed auxin signaling (miR393) and analyzing the expression of auxin receptors in wild-type inoculated plants, we provide evidence that auxin signaling in plants is necessary for the growth promotion effects produced by B. phytofirmans. The interplay between ethylene and auxin signaling was also confirmed by the response of the plant to a 1-aminocyclopropane-1-carboxylate deaminase bacterial mutant strain.
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Kim, Ho-Bin, Min-Ju Park, Hee-Chan Yang, Dong-Shan An, Hai-Zhu Jin, and Deok-Chun Yang. "Burkholderia ginsengisoli sp. nov., a β-glucosidase-producing bacterium isolated from soil of a ginseng field." International Journal of Systematic and Evolutionary Microbiology 56, no. 11 (November 1, 2006): 2529–33. http://dx.doi.org/10.1099/ijs.0.64387-0.
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A bacterial strain (designated KMY03T) that possesses β-glucosidase activity was isolated from soil from a ginseng field in South Korea and was characterized in order to determine its taxonomic position. The bacterium was found to comprise Gram-negative, rod-shaped, motile cells with unipolar polytrichous flagella. On the basis of 16S rRNA gene sequence similarity, strain KMY03T was shown to belong to the family Burkholderiaceae of the Betaproteobacteria, being most closely related to Burkholderia caledonica LMG 19076T (97.8 %), Burkholderia terricola LMG 20594T (97.5 %), Burkholderia xenovorans LMG 21463T (97.4 %) and Burkholderia phytofirmans LMG 22146T (97.3 %). Chemotaxonomic data (major ubiquinone, Q-8; major fatty acids, C17 : 0 cyclo, C16 : 0, C19 : 0 cyclo ω8c and summed feature 2) supported the affiliation of the novel strain with the genus Burkholderia. The results of DNA–DNA hybridizations and physiological and biochemical tests allowed the strain to be differentiated genotypically and phenotypically from Burkholderia species with validly published names. On the basis of these data, strain KMY03T represents a novel species of the genus Burkholderia, for which the name Burkholderia ginsengisoli sp. nov. is proposed. The type strain is KMY03T (=KCTC 12389T=NBRC 100965T).
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Sessitsch, A., T. Coenye, A. V. Sturz, P. Vandamme, E. Ait Barka, J. F. Salles, J. D. Van Elsas, et al. "Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties." International Journal of Systematic and Evolutionary Microbiology 55, no. 3 (May 1, 2005): 1187–92. http://dx.doi.org/10.1099/ijs.0.63149-0.
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A Gram-negative, non-sporulating, rod-shaped, motile bacterium, with a single polar flagellum, designated strain PsJNT, was isolated from surface-sterilized onion roots. This isolate proved to be a highly effective plant-beneficial bacterium, and was able to establish rhizosphere and endophytic populations associated with various plants. Seven related strains were recovered from Dutch soils. Based on 16S rRNA gene sequence data, strain PsJNT and the Dutch strains were identified as representing a member of the genus Burkholderia, as they were closely related to Burkholderia fungorum (98·7 %) and Burkholderia phenazinium (98·5 %). Analysis of whole-cell protein profiles and DNA–DNA hybridization experiments confirmed that all eight strains belonged to a single species. Strain PsJNT had a DNA G+C content of 61·0 mol%. Only low levels of DNA–DNA hybridization to closely related species were found. Qualitative and quantitative differences in fatty acid composition between strain PsJNT and closely related species were identified. The predominant fatty acids in strain PsJNT were 16 : 0, 18 : 1ω7c and summed feature 3 (comprising 16 : 1ω7c and/or iso-15 : 0 2-OH). Isolate PsJNT showed high 1-aminocyclopropane-1-carboxylate deaminase activity and is therefore able to lower the ethylene level in a developing or stressed plant. Production of the quorum-sensing signal compound 3-hydroxy-C8-homoserine lactone was detected. Based on the results of this polyphasic taxonomic study, strain PsJNT and the seven Dutch isolates are considered to represent a single, novel species, for which the name Burkholderia phytofirmans sp. nov. is proposed. The type strain is strain PsJNT (=LMG 22146T=CCUG 49060T).
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Aguilar Diaz, Trinidad, Vincenzo Bertolini, Guillermo Carrillo Castañeda, Griselda Karina Guillén Navarro, Luz Verónica García Fajardo, and Ricardo Alberto Castro Chan. "Rizobacterias promotoras de crecimiento en Guarianthe skinneri (Orchidaceae)." Revista de Biología Tropical 66, no. 3 (July 4, 2018): 953. http://dx.doi.org/10.15517/rbt.v66i3.30638.
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The Guarianthe skinneri orchid is included in NOM-059-ECOL-2010, Mexico standard as an endangered species. In order to study PGPR (promoting growth plant rhizobacteria) from this orchid, 10 roots were collected from different plants to isolate bacteria associated with the roots, which were analyzed by in vitro tests such as: production of AIA, nitrogen fixation, interaction with the mycorrhizal fungus Thanatephorus sp. strain RG26 and phosphate solubilization. We obtain 71 bacterial isolates, 10 strains of them were characterized by sequencing with the 16d rDNA marker identifying six bacteria: Sphingomonas sp. Sinorhizobium sp. Bacillus sp. Nocardia cerradoensis, Bacillus megaterium and Burkholderia phytofirmans. We observed that the bacterium Sinorhizobium sp. produced a greater amount of AIA (69.189 μg/ml) and Bacillus sp. performed greater acetylene reduction (10.251 nmol cultivo/96h). In the interactions of the bacteria and the fungus RG26, four categories were presented (extremely positive, positive, antagonism 50-50 and inhibition). In relation to the solubilization of phosphate, Burkholderia phytofirmans presented higher IS after 48 and 96 hr with an IS of 3.11 and 3.48, respectively. The results indicate that Bacillus sp. it could have the best characteristics to promote the development of the G. skinneri orchid by inoculating seeds and seedlings.
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Fernandez, Olivier, Andreas Theocharis, Sophie Bordiec, Regina Feil, Lucile Jacquens, Christophe Clément, Florence Fontaine, and Essaid Ait Barka. "Burkholderia phytofirmans PsJN Acclimates Grapevine to Cold by Modulating Carbohydrate Metabolism." Molecular Plant-Microbe Interactions® 25, no. 4 (April 2012): 496–504. http://dx.doi.org/10.1094/mpmi-09-11-0245.
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Low temperatures damage many temperate crops, including grapevine, which, when exposed to chilling, can be affected by symptoms ranging from reduced yield up to complete infertility. We have previously demonstrated that Burkholderia phytofirmans PsJN, a plant growth-promoting rhizobacteria (PGPR) that colonizes grapevine, is able to reduce chilling-induced damage. We hypothesized that the induced tolerance may be explained at least partly by the impact of bacteria on grapevine photosynthesis or carbohydrate metabolism during cold acclimation. To investigate this hypothesis, we monitored herein the fluctuations of photosynthesis parameters (net photosynthesis [Pn], intercellular CO2 concentration, stomatal conductances, ΦPSII, and total chlorophyll concentration), starch, soluble sugars (glucose, fructose, saccharose, mannose, raffinose, and maltose), and their precursors during 5 days of chilling exposure (4°C) on grapevine plantlets. Bacterization affects photosynthesis in a non–stomatal dependent pattern and reduced long-term impact of chilling on Pn. Furthermore, all studied carbohydrates known to be involved in cold stress tolerance accumulate in non-chilled bacterized plantlets, although some of them remained more concentrated in the latter after chilling exposure. Overall, our results suggest that modification of carbohydrate metabolism in bacterized grapevine plantlets may be one of the major effects by which this PGPR reduces chilling-induced damage.
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Rusch, Antje, Shaer Islam, Pratixa Savalia, and Jan P. Amend. "Burkholderia insulsa sp. nov., a facultatively chemolithotrophic bacterium isolated from an arsenic-rich shallow marine hydrothermal system." International Journal of Systematic and Evolutionary Microbiology 65, Pt_1 (January 1, 2015): 189–94. http://dx.doi.org/10.1099/ijs.0.064477-0.
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Enrichment cultures inoculated with hydrothermally influenced nearshore sediment from Papua New Guinea led to the isolation of an arsenic-tolerant, acidophilic, facultatively aerobic bacterial strain designated PNG-AprilT. Cells of this strain were Gram-stain-negative, rod-shaped, motile and did not form spores. Strain PNG-AprilT grew at temperatures between 4 °C and 40 °C (optimum 30–37 °C), at pH 3.5 to 8.3 (optimum pH 5–6) and in the presence of up to 2.7 % NaCl (optimum 0–1.0 %). Both arsenate and arsenite were tolerated up to concentrations of at least 0.5 mM. Metabolism in strain PNG-AprilT was strictly respiratory. Heterotrophic growth occurred with O2 or nitrate as electron acceptors, and aerobic lithoautotrophic growth was observed with thiosulfate or nitrite as electron donors. The novel isolate was capable of N2-fixation. The respiratory quinones were Q-8 and Q-7. Phylogenetically, strain PNG-AprilT belongs to the genus Burkholderia and shares the highest 16S rRNA gene sequence similarity with the type strains of Burkholderia fungorum (99.8 %), Burkholderia phytofirmans (98.8 %), Burkholderia caledonica (98.4 %) and Burkholderia sediminicola (98.4 %). Differences from these related species in several physiological characteristics (lipid composition, carbohydrate utilization, enzyme profiles) and DNA–DNA hybridization suggested the isolate represents a novel species of the genus Burkholderia , for which we propose the name Burkholderia insulsa sp. nov. The type strain is PNG-AprilT ( = DSM 28142T = LMG 28183T).
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Yang, Aizheng, Saqib Saleem Akhtar, Qiang Fu, Muhammad Naveed, Shahid Iqbal, Thomas Roitsch, and Sven-Erik Jacobsen. "Burkholderia Phytofirmans PsJN Stimulate Growth and Yield of Quinoa under Salinity Stress." Plants 9, no. 6 (May 26, 2020): 672. http://dx.doi.org/10.3390/plants9060672.
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One of the major challenges in agriculture is to ensure sufficient and healthy food availability for the increasing world population in near future. This requires maintaining sustainable cultivation of crop plants under varying environmental stresses. Among these stresses, salinity is the second most abundant threat worldwide after drought. One of the promising strategies to mitigate salinity stress is to cultivate halotolerant crops such as quinoa. Under high salinity, performance can be improved by plant growth promoting bacteria (PGPB). Among PGPB, endophytic bacteria are considered better in stimulating plant growth compared to rhizosphere bacteria because of their ability to colonize both in plant rhizosphere and plant interior. Therefore, in the current study, a pot experiment was conducted in a controlled greenhouse to investigate the effects of endophytic bacteria i.e., Burkholderia phytofirmans PsJN on improving growth, physiology and yield of quinoa under salinity stress. At six leaves stage, plants were irrigated with saline water having either 0 (control) or 400 mM NaCl. The results indicated that plants inoculated with PsJN mitigated the negative effects of salinity on quinoa resulting in increased shoot biomass, grain weight and grain yield by 12%, 18% and 41% respectively, over un-inoculated control. Moreover, inoculation with PsJN improved osmotic adjustment and ion homeostasis ability. In addition, leaves were also characterized for five key reactive oxygen species (ROS) scavenging enzyme in response to PsJN treatment. This showed higher activity of catalase (CAT) and dehydroascobate reductase (DHAR) in PsJN-treated plants. These findings suggest that inoculation of quinoa seeds with Burkholderia phytofirmans PsJN could be used for stimulating growth and yield of quinoa in highly salt-affected soils.
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Naveed, Muhammad, M. Baqir Hussain, Zahir A. Zahir, Birgit Mitter, and Angela Sessitsch. "Drought stress amelioration in wheat through inoculation with Burkholderia phytofirmans strain PsJN." Plant Growth Regulation 73, no. 2 (November 30, 2013): 121–31. http://dx.doi.org/10.1007/s10725-013-9874-8.
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Wang, Qi, Shan Gao, Xiang Ma, Xinxin Mao, Linyan He, and Xiafang Sheng. "Distinct mineral weathering effectiveness and metabolic activity between mineral-weathering bacteria Burkholderia metallica F22 and Burkholderia phytofirmans G34." Chemical Geology 489 (June 2018): 38–45. http://dx.doi.org/10.1016/j.chemgeo.2018.05.016.
Full textDissertations / Theses on the topic "Burkholderia phytofirmans"
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Wang, Bingxue. "Burkholderia phytofirmans strain PsJN effects on drought resistance, physiological responses and growth of switchgrass." Diss., Virginia Tech, 2015. http://hdl.handle.net/10919/51358.
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To decrease dependency of fossil fuels and avoid direct competition with food crops, massive research efforts are investigating next-generation cellulose biofuel crops such as switchgrass (Panicum virgatum). A low-input, sustainable switchgrass production could be achieved by reducing traditional management practices though applying plant growth promoting rhizobacteria (PGPR), of which our understanding is still rather limited.
To elucidate physiological mechanisms behind PGPR's beneficial effects, we inoculated switchgrass seedlings with Burkholderia phytofirmans strain PsJN. Two experiments were conducted to determine the initial and long-term responses of switchgrass to PsJN inoculation by tracking growth and leaf physiology. In a third experiments, we tested the effects of PsJN on growth and leaf-level physiology of switchgrass under a moderate pre-drought conditioning and a successive severe drought stress.
PsJN inoculation increased biomass and promoted elongation of shoots within 17 days following inoculation. The enhanced root growth in PsJN inoculated plants lagged behind the shoot response, resulting in greater allocation to aboveground growth (p=0.0041). Lower specific root length (p=0.0158) and higher specific leaf weight (p=0.0029) were also observed in PsJN inoculated seedlings, indicating advanced development. Photosynthetic rates (Ps) were higher in PsJN inoculated seedlings after 17 days (54%, p=0.0016), which were related to higher stomatal conductance, greater water use efficiency, and lower non-stomatal limitation of Ps. These rapid changes in leaf physiology are at least partially responsible for switchgrass growth enhancement from PsJN treatment. The early growth enhancement in PsJN inoculated switchgrass linearly decreased with plant age. PsJN inoculation increased Ps of upper canopy leaves by 13.6% but reduced Ps of lower canopy leaves by 8.2%. Accelerated leaf senescence and early flowering were observed in PsJN-inoculated switchgrass, which might contribute to slightly lower aboveground biomass at final harvesting. Drought preconditioning increased Ps of PsJN-inoculated switchgrass during a later severe drought; whereas, control switchgrass only benefited from drought preconditioning when leaf water potential dropped below -1 MPa.
This study verified early growth enhancement and accelerated development of switchgrass due to PsJN inoculation. Rapid improvement in leaf physiology is related to enhanced productivity. PsJN inoculation also improve drought tolerance of switchgrass.Ph. D.
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Theocharis, Andreas I. "Physiological responses of Burkholderia phytofirmans strain PsJN colonized plantlets of grapevine (Vitis vinifera L. ) to low non-freezing temperatures." Reims, 2010. http://theses.univ-reims.fr/sciences/2010REIMS003.pdf.
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Burkholderia phytofirmans strain PsJN has been well characterized as a Plant Growth Promoting Rhizobacteria (PGPR) that triggers induced resistance in grapevine against fungal pathogens. To better understand the interaction between grapevine and B. Phytofirmans strain PsJN, changes in the expression pattern of different defence related genes were investigated in Chardonnay grapevine leaves after root inoculation with PsJN strain. B. Phytofirmans induced a systemic spread of a signal from roots to leaves after root inoculation with bacteria, a phenomenon referred to as Induced Systemic Resistance (ISR). The expression pattern of well-characterized grapevine defence genes was also monitored in grapevine plantlets bacterized four weeks earlier, and subjected to low non-freezing temperature. Results report that PsJN induces earlier and/or higher transcript accumulation of defence genes in bacterized plantlets upon low non-freezing temperatures according to the phenomenon of priming. Investigation of several biochemical parameters reveals that bacterized grapevine plantlets are in a primed physiological state able to increase their sugar, starch and proline levels upon low non-freezing temperatures while the analysis of membrane lipid peroxidation markers indicates a faster degradation of aldehydes, malonaldehyde and hydrogen peroxide beyond one week, addressing the better adaptation of bacterized plantlets than non-bacterized plantlets to low non-freezing temperature. In conclusion, PsJN strain is an ISR-inducing PGPR able to stimulate grapevine defence mechanism by priming physiological responses critical to acclimation under low non-freezing temperaturesBurkholderia phytofirmans souche PsJN a été caractérisée comme une rhizobactérie promotrice de la croissance des plantes (PGPR) capable d’induire la résistance de la vigne contre certains phypathogènes. Pour mieux comprendre l’interaction entre la vigne et la souche PsJN, le profil de l’expression de différents gènes de défenses a été analysé au niveau des feuilles de vitroplants après l’inoculation des racines par la bactérie. Les résultats montrent que la souche PsJN induit la propagation d’un signal systémique, des racines vers les feuilles, caractéristique d’une résistance systémique induite (ISR). L’expression de gènes de défenses a également été suivie au niveau des vitroplants bactérisés 4 semaines avant leur traitement par les basses températures. Les résultats montrent une accumulation précoce et/ou intense des transcrits de ces gènes au niveau des vitroplants bactérisés et soumis aux basses températures selon le phénomène de potentialisation. Cet état de potentialisation permet également aux vitroplants bactérisés d’augmenter la teneur en sucres solubles, amidon et proline après le stress thermique. L’étude des marqueurs de peroxydation membranaire a montré une dégradation plus rapide des aldéhydes, du malondialdéhyde et du peroxyde d’hydrogène, une semaine après le début du stress froid, indiquant ainsi une meilleure adaptation des vitroplants bactérisés aux basses températures. En conclusion, B. Phytofirmans souche PsJN est une PGPR inductrice de l’ISR qui est capable de stimuler les mécanismes de défense de la vigne via un état de potentialisation qui lui permettrait une acclimatation en condition de basses températures
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Su, Fan. "Modifications physiologiques induites par Burkholderia phytofirmans chez Arabidopsis thaliana. Applications à la protection contre les stress biotique et abiotique." Thesis, Reims, 2015. http://www.theses.fr/2015REIMS032.
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La PGPR Burkholderia phytofirmans PsJN (Bp) stimule la croissance de diverses plantes et les protège également contre certains stress environnementaux. L’objectif des travaux a été d’approfondir les connaissances sur l’interaction Bp-plante, en se focalisant sur l’aspect physiologique et métabolique des feuilles d’Arabidopsis thaliana. Nous avons également déterminé les mécanismes impliqués dans la réponse des feuilles suite à l’inoculation de cette bactérie lors d’un stress abiotique (froid) ou biotique (Pseudomonas syringae pv. tomato DC3000, Pst).Nos résultats montrent que l’induction de la promotion de croissance d’A. thaliana par Bp pourrait être liée à l’accumulation des teneurs en métabolites primaires (acides aminés, glucides solubles et vitamines) et la variation du niveau des hormones dans les feuilles. La physiologie et le métabolisme des feuilles sont modifiés localement et de façon distale par la colonisation épi- et endophytique de Bp. De plus, les modifications des taux de métabolites sont plus marquées après une interaction plante-bactéries relativement longue. Par ailleurs, l’inoculation de Bp peut réduire les dommages sur l’activité photosynthétique dus au froid par une limitation non-stomatique de la photosynthèse et l’accumulation de pigments photosynthétiques. Enfin, la présence de Bp entraîne localement un retard dans le développement initial de Pst. Cependant, l’inoculation par Bp ne protège pas l’appareil photosynthétique lors d’une attaque par Pst. Ces travaux soulignent donc que le temps de présence et la localisation d’une PGPR dans une plante influencent la physiologie, le métabolisme et la tolérance aux stress de cette même planteEndophytic PGPR Burkholderia phytofirmans PsJN (Bp) promotes growth of various plants and triggers protection against several environmental stresses. To get more insights into the interaction between plant and Bp, we focused on leaf physiological and metabolic aspects of Arabidopsis thaliana. We also determined the mechanisms involved in the defense of leaves after inoculation of the bacteria followed by an abiotic (cold) or a biotic (Pseudomonas syringae pv. tomato DC3000, Pst) stress. Our results show that the induction of growth promotion of A. thaliana by Bp could be related to the accumulation of primary metabolite levels (amino acids, soluble carbohydrates and vitamins) and to the variation of hormone levels in the leaves. Leaf physiology and metabolism are changed locally and distally by Bp epi- and endophytic colonization. In addition, changes in metabolite levels are more pronounced after a relatively long interaction between plant and bacteria.Moreover, Bp inoculation can also reduce cold injury on the photosynthetic activity by a non-stomatal limitation of photosynthesis and accumulation of photosynthetic pigments. Finally, the local presence of Bp causes a delay in the development of Pst, but only in the early stages of the infection. However, the inoculation with Bp does not protect the photosynthetic apparatus during Pst attack.Thus, our results emphasize that the time of presence of a PGPR and his location in the plant could influence the plant physiology and stress tolerance
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Compant, Stéphane Clément Christophe Ait Barka Essaïd. "Interaction entre la vigne, Vitis vinifera L., et une bactérie endophytique, Burkholderia phytofirmans souche PsJN : colonisation, induction de défenses et résistance systémique contre Botrytis cinerea." Reims : S.C.D. de l'Université, 2007. http://scdurca.univ-reims.fr/exl-doc/GED00000953.pdf.
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Compant, Stéphane. "Interaction entre la vigne, Vitis vinifera L. , et une bactérie endophytique, Burkholderia phytofirmans souche PsJN : colonisation, induction de défenses et résistance systémique contre Botrytis cinerea." Reims, 2007. http://theses.univ-reims.fr/exl-doc/GED00000953.pdf.
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Lors de ces travaux, l’interaction entre la vigne, Vitis vinifera L. , et une PGPR endophytique, Burkholderia phytofirmans souche PsJN, a été étudiée. Ceci a permis de caractériser des phénomènes de colonisation bactérienne, des réponses de défense de la plante, ainsi qu’une résistance systémique induite (ISR) permettant une protection contre l’agent pathogène Botrytis cinerea Pers. L’association entre la vigne et la souche PsJN a tout d’abord été étudiée en conditions gnotobiotiques en utilisant des plantules in vitro, la souche bactérienne de type sauvage ou des dérivés génétiques (PsJN::gfp2x et PsJN::gusA11). Ceci a permis de déterminer une colonisation épi- et endophytique des racines de vigne par la souche PsJN ainsi qu’une migration de la bactérie de l’intérieur des racines jusqu’aux feuilles, par le biais du flux d’évapo-transpiration de la plante via les vaisseaux du xylème. La colonisation de la vigne par la souche PsJN a ensuite été étudiée en conditions non stériles en utilisant des boutures fructifères dans le but de décrire une possible colonisation des inflorescences. Les résultats obtenus ont permis de montrer une colonisation épi- et endophytique du système racinaire par la bactérie et ensuite, une migration des racines jusque la rafle et les jeunes baies, en présence d’autres micro-organismes. La mise en place de composés de défense ainsi qu’une ISR induite par la souche PsJN a été ensuite déterminée sur des boutures fructifères. Ceci a été caractérisé avant sa progression systémique et avec des conditions plus stériles. Des événements précoces tels que l’accumulation de peroxyde d’hydrogène et d’oxyde nitrique, ainsi que la synthèse de composés phénoliques ont été caractérisés au niveau racinaire. De plus, il est apparu, d’après des résultats préliminaires, que la souche PsJN induit, au niveau local ainsi qu’au niveau systémique, des gènes codants des protéines PR dont la signalisation dépend de la voie de l’acide salicylique et / ou de l’acide jasmonique. Ceci a permis de suggérer que la souche bactérienne induit des mécanismes communs des phénomènes de résistance systémique acquise (SAR) et d’ISR. Ces phénomènes permettent, par la suite, une protection de la vigne au niveau floral vis à vis de l’infection causée par B. Cinerea PersThe interaction between grapevine, Vitis vinifera L. , and an endophytic PGPR, Burkholderia phytofirmans strain PsJN, has been studied in this work. This has allowed to characterize phenomenons of bacterial colonization, some plant defence responses as well as induced systemic resistance (ISR) confering protection against the phytopathogen Botrytis cinerea Pers. Association between grapevine and strain PsJN has been studied, firstly, under gnotobotic conditions by using in vitro plantlets, PsJN wild-type strain or some genetically derivatives (PsJN::gfp2x and PsJN::gusA11). This has allowed to determine epi- and endophytic colonization of grapevine roots by strain PsJN as well as a migration of the bacterium from the endorhiza to the leaves, mediated by the plant transpiration stream via xylem vessels. Grapevine colonization by strain PsJN has been then monitored under non-sterile conditions by using fruiting cuttings, with a special emphasis on putative inflorescence colonization. Results have demonstrated an epi- and an endophytic colonization of the root system by strain PsJN and then, its spreading from the endorhiza to grape inflorescence stalk and to young berries, even in presence of other microorganisms. Plant defence compounds as well as a ISR induced by strain PsJN has been then determined on fruiting cuttings. This has been characterized before its systemic spreading inside plants and with some more sterile conditions. Some early events such as hydrogen peroxide and nitric oxide accumulations as well as phenolic compound synthesis have been characterized at the root level. In addition, preliminary results have demonstrated that strain PsJN induces locally and systemically some PR-encoding genes, dependent of salicylate and/or jasmonate signaling pathways. This has allowed to suggest that this bacterium induces common mechanisms of Systemic Acquired Resistance (SAR) and of ISR phenomenons. These phenomenons allow then a protection of grapevine flowers against infection caused by B. Cinerea Pers
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Bordiec, Sophie. "Interaction entre la vigne (Vitis vinifera L. ) et une bactérie PGPR, Burkholderia phytofirmans souche PsJN : mécanismes de défense impliqués lors de la perception de la bactérie par la plante, et lors de l’établissement de la protection contre le froid et la pourriture grise." Reims, 2010. http://theses.univ-reims.fr/sciences/2010REIMS030.pdf.
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Burkholderia phytofirmans souche PsJN (bactérie de type PGPR) est capable de coloniser le système racinaire de la vigne et de diffuser dans la plante. Au cours de mes travaux de recherche, nous avons tout d’abord montré que la bactérie est perçue par les cellules de vigne, cette perception induisant des réponses de défense. Par ailleurs, l’interaction entre la souche PsJN et la vigne confère à la plante une protection vis-à-vis de Botrytis cinerea et d’une exposition à 4°C. Afin de mieux caractériser cette protection, nous avons analysé la mise en place de composés de défense en réponse à ces deux stress dans des vitroplants entièrement colonisés par la bactérie. Les plants bactérisés montrent une tolérance envers le froid. Cette protection est associée à la potentialisation de l’expression de gènes impliqués dans les réponses de défense, une accumulation de proline, ainsi qu’une activation plus rapide des systèmes de détoxification de l’H2O2 et des aldéhydes. Par ailleurs, il a été démontré que la souche PsJN protège la vigne contre B. Cinerea. L’étude de l’expression de plusieurs gènes de défense n’a pu révéler la potentialisation de leurs expressions. Par contre, cette protection a été associée à une accumulation plus importante de phytoalexines. Ces travaux démontrent donc que la relation bénéfique qui s’établit entre la vigne et la souche PsJN, se manifeste par une protection contre le froid ou B. Cinerea via le phénomène de potentialisation. Cependant, la présence de la bactérie localement semble être un facteur déterminant pour l’établissement de la résistance dans la mesure où aucune protection contre ces stress n’a été obtenue en condition systémiqueThe PGPR Burkholderia phytofirmans strain PsJN is able to colonize grapevine roots and to diffuse to leaves, inflorescences and berries. The present work aimed to better characterize this interaction. Firstly, our results showed that interaction between grapevine cell suspensions and this bacterium induced plant defense responses. This demonstrated that strain PsJN was locally recognized by the plant cells, which triggered plant immunity modifications. Interaction between grapevine and strain PsJN also confers to the plant a protection against Botrytis cinerea and cold stress (4°C). In order to better characterize this protection, induced defense responses were analyzed in fully bacterized plantlets submitted to these stress. The bacterium induced a protection against cold stress. This protection is correlated with priming of some stress-related gene expressions, an accumulation of proline, and a faster activation of H2O2 and aldehyde scavenging systems. Moreover, it was demonstrated that B. Phytofirmans strain PsJN protects grapevine against B. Cinerea. Analysis of defense-related gene expressions showed no priming of their expressions. Nevertheless, the observed protection was correlated with a higher phytoalexin accumulation. Thus, this work demonstrated that the beneficial relationship between grapevine and strain PsJN induced a protection against cold exposure (4°C) or B. Cinerea attacks, which was correlated with priming phenomenon. Nevertheless, preliminary studies showed that the presence of strain PsJN locally seemed to be an important factor for resistance establishment, since no protection against these stresses was observed in a systemic context
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Goordial, Jacqueline. "Characterization of a Novel Chlorobenzoate Degrading bacterium: Burkholderia phytofirmans OLGA172, Isolated from a Pristine Environment." Thesis, 2010. http://hdl.handle.net/1807/25600.
Full textAbstract:
Burkholderia phytofirmans OLGA172 is a chlorobenzoate (CBA) degrading bacterium, known to frequently lose the ability to degrade CBA in the lab. OLGA172 carries the complete set of genes for chlorocatechol degradation (an intermediate in CBA metabolism), tfdCDEF, as well as several integrases associated with DNA mobility in proximity to these genes. In this study, putative CBA degradative genes were identified in OLGA172, and an imbalance in regulation between the tfdCDEF and CBA degradative genes identified as a cause for incomplete CBA metabolism in this strain. Additionally, expression of the integrase genes was observed to occur constitutively. The role of this expression was not determined but hypothesized to be related to the phenotypic instability seen in OLGA172. Characterization of the strain was carried out with the aim to determine the ecological niche OLGA172 occupies naturally. Results of characterization are discussed in the context of evolution of the chlorocatechol degradative genes.
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Jin, Soulbee. "Evidence of Mobility in the 3-chlorobenzoate Degradative Genes in a Pristine Soil Isolate, Burkholderia phytofirmans OLGA172." Thesis, 2010. http://hdl.handle.net/1807/32205.
Full textAbstract:
The genome of B. phytofirmans OLGA172 has been sequenced by Next Generation sequencing methods. Over 42 kbp of its genome surrounding its 3CBA degradative genes, tfdCIDIEIFI, was assembled and annotated. The most important method used was the synteny method, which implies homology between the genes, and descent from a common ancestor (Guttman, 2008). The conserved gene order between B. phytofirmans PsJN, B. xenovorans LB400, and OLGA172 was used as a confirmation of annotation through BLASTn, enabled closing of the gaps in the sequencing data, and allowed prediction of genes further downstream. Though the whole genome is not yet assembled, a very significant region carrying a concentrated area of mobile genetic elements (MGE) has been found to surround the degradative genes in OLGA172. This thesis details the sequence evidence that, upon examination of closely related strains, OLGA172 and its related strain from pristine soils may be the ancestral chlorobenzoate degraders.
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Sun, Yili. "The role of ACC deaminase in plant growth promotion by the endophytic bacterium Burkholderia phytofirmans PsJN." Thesis, 2008. http://hdl.handle.net/10012/3832.
Full textAbstract:
The endophytic bacterium Burkholderia phytofirmans PsJN has been previously shown to promote plant growth. This bacterium produces siderophores, indoleacetic acid (IAA) and the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, all of which have previously been implicated in the promotion of plant growth by bacteria. Following isolation of the ACC deaminase gene (acdS), AcdS deficient mutants of PsJN were generated. One mutant contains a tetracycline resistance gene inserted into acdS, and the other mutant contains a deletion in the acdS gene. Both of the mutants showed no detectable ACC deaminase activity, produced a decreased level of siderophores and an increased amount of IAA compared to the wild-type, and lost the ability to promote canola root elongation. In addition, the GFP-labeled acdS deletion mutant colonized plant interior surfaces somewhat less efficiently than the GFP-labeled wild-type strain.
Book chapters on the topic "Burkholderia phytofirmans"
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Mitter, Birgit, Alexandra Petric, Patrick SG Chain, Friederike Trognitz, Jerzy Nowak, Stéphane Compant, and Angela Sessitsch. "Genome Analysis, Ecology, and Plant Growth Promotion of the Endophyte Burkholderia phytofirmans Strain PsJN." In Molecular Microbial Ecology of the Rhizosphere, 865–74. Hoboken, NJ, USA: John Wiley & Sons, Inc., 2013. http://dx.doi.org/10.1002/9781118297674.ch81.
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