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Journal articles on the topic 'Butyl methyl ether In situ bioremediation'

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1

Hristova, Krassimira R., Christian M. Lutenegger, and Kate M. Scow. "Detection and Quantification of Methyl tert-Butyl Ether-Degrading Strain PM1 by Real-Time TaqMan PCR." Applied and Environmental Microbiology 67, no. 11 (November 1, 2001): 5154–60. http://dx.doi.org/10.1128/aem.67.11.5154-5160.2001.

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ABSTRACT The fuel oxygenate methyl tert-butyl ether (MTBE), a widely distributed groundwater contaminant, shows potential for treatment by in situ bioremediation. The bacterial strain PM1 rapidly mineralizes and grows on MTBE in laboratory cultures and can degrade the contaminant when inoculated into groundwater or soil microcosms. We applied the TaqMan quantitative PCR method to detect and quantify strain PM1 in laboratory and field samples. Specific primers and probes were designed for the 16S ribosomal DNA region, and specificity of the primers was confirmed with DNA from 15 related bacterial strains. A linear relationship was measured between the threshold fluorescence (C T ) value and the quantity of PM1 DNA or PM1 cell density. The detection limit for PM1 TaqMan assay was 2 PM1 cells/ml in pure culture or 180 PM1 cells/ml in a mixture of PM1 withEscherichia coli cells. We could measure PM1 densities in solution culture, groundwater, and sediment samples spiked with PM1 as well as in groundwater collected from an MTBE bioaugmentation field study. In a microcosm biodegradation study, increases in the population density of PM1 corresponded to the rate of removal of MTBE.
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2

d’Errico, Giada, Veronica Aloj, Valeria Ventorino, Assunta Bottiglieri, Ernesto Comite, Alberto Ritieni, Roberta Marra, et al. "Methyl t-butyl ether-degrading bacteria for bioremediation and biocontrol purposes." PLOS ONE 15, no. 2 (February 21, 2020): e0228936. http://dx.doi.org/10.1371/journal.pone.0228936.

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3

Hu, C., K. Acuna-Askar, and A. J. Englande. "Bioremediation of methyl tertiary-butyl ether (MTBE) by an innovative biofilter." Water Science and Technology 49, no. 1 (January 1, 2004): 87–94. http://dx.doi.org/10.2166/wst.2004.0026.

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Methyl tertiary-butyl ether (MTBE) is a synthetic chemical used in unleaded gasoline as an additive to reduce levels of ozone and carbon monoxide from auto exhaust. Due to its chemical and recalcitrant properties, MTBE has caused groundwater contamination worldwide. A laboratory-scale biofilter made of a natural fiber (kenaf) mat and inoculated with MTBE-degrading microorganisms, was evaluated for MTBE removal efficiency. Operational parameters of oxygen flow rate, hydraulic retention time (HRT), yeast extract and initial MTBE concentration were varied and MTBE removal efficiencies determined. Four kinetic models were evaluated to describe the MTBE removal in the reactor. Formaldehyde and tertiary butyl alcohol (the most two reported MTBE biodegradation byproducts) were not found in the effluent; instead, carbon dioxide was monitored as the end product based on the results of a metabolic mass balance evaluation. Toxicity of treated effluent was evaluated by employing the Microtox acute toxicity test and comparing that to the influent.
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4

Lalevic, Blazo, Jelena Jovic, Vera Raicevic, Igor Kljujev, Dragan Kikovic, and Saud Hamidovic. "Biodegradation of methyl tert-butyl ether by Kocuria sp." Chemical Industry 66, no. 5 (2012): 717–22. http://dx.doi.org/10.2298/hemind120110019l.

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Methyl tert-butyl ether (MTBE) has been used to replace the toxic compounds from gasoline and to reduce emission of air pollutants. Due to its intensive use, MTBE has become one of the most important environment pollutants. The aim of this paper is isolation and identification of the bacteria from wastewater sample of ?HIP Petrohemija? Pancevo (Serbia), capable of MTBE biodegradation. The results of the investigation showed that only the bacterial isolate 27/1 was capable of growth on MTBE. The result of sequence analyzes of 16S rDNA showed that this bacterial isolate belongs to the Kocuria sp. After the incubation period of 86 days, the degradation rates of initial MTBE concentration of 25 and 125 ?g/ml were 55 and 36%, respectively. These results indicated that bacteria Kocuria sp. is successfully adapted on MTBE and can be potentially used in bioremediation of soils and waters contaminated with MTBE.
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5

Guisado, I. M., J. Purswani, L. Catón-Alcubierre, J. González-López, and C. Pozo. "Toxicity and biofilm-based selection for methyl tert-butyl ether bioremediation technology." Water Science and Technology 74, no. 12 (October 4, 2016): 2889–97. http://dx.doi.org/10.2166/wst.2016.461.

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Extractive membrane biofilm reactor (EMBFR) technology offers productive solutions for volatile and semi-volatile compound removal from water bodies. In this study, the bacterial strains Paenibacillus etheri SH7T (CECT 8558), Agrobacterium sp. MS2 (CECT 8557) and Rhodococcus ruber strains A5 (CECT 8556), EE6 (CECT 8612) and EE1 (CECT 8555), previously isolated from fuel-contaminated sites, were tested for adherence on tubular semipermeable membranes in laboratory-scale systems designed for methyl tert-butyl ether (MTBE) bioremediation. Biofilm formation on the membrane surface was evaluated through observation by field-emission scanning electron microscope (FESEM) as well as the acute toxicity (as EC50) of the bacterial growth media. Moreover, extracellular polymeric substance (EPS) production for each strain under different MTBE concentrations was measured. Strains A5 and MS2 were biofilm producers and their adherence increased when the MTBE flowed through the inner tubular semipermeable membrane. No biofilm was formed by Paenibacillus etheri SH7T, nevertheless, the latter and strain MS2 exhibited the lowest toxicity after growth on the EMBFR. The results obtained from FESEM and toxicity analysis demonstrate that bacterial strains R. ruber EE6, A5, P. etheri SH7T and Agrobacterium sp. MS2 could be excellent candidates to be used as selective inocula in EMBFR technology for MTBE bioremediation.
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6

Yousefi, Zabihollah, Zeinab Tahernezhad, Seyed Noroddin Mousavinasab, Reza Safari, and Ahmadreza Bekhradnia. "Bioremediation of methyl tertiary-butyl ether (MTBE) by three pure bacterial cultures." Environmental Health Engineering and Management 5, no. 2 (June 15, 2018): 123–28. http://dx.doi.org/10.15171/ehem.2018.17.

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7

Chen, Colin S., Chien-Jun Tien, and Kai-Van Zhan. "Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater." Journal of Soil and Groundwater Environment 19, no. 5 (October 31, 2014): 9–17. http://dx.doi.org/10.7857/jsge.2014.19.5.009.

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8

Volpe, Angela, Guido Del Moro, Simona Rossetti, Valter Tandoi, and Antonio Lopez. "Enhanced bioremediation of methyl tert-butyl ether (MTBE) by microbial consortia obtained from contaminated aquifer material." Chemosphere 75, no. 2 (April 2009): 149–55. http://dx.doi.org/10.1016/j.chemosphere.2008.12.053.

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9

Matusiak, Grazyna. "1,3-Dipolar Cycloaddition Reactions of the Ylide Derived from 6-Phenacyl-benzo[f][1,7]naphthyridinium Bromide." Australian Journal of Chemistry 52, no. 2 (1999): 149. http://dx.doi.org/10.1071/c98109.

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The 1,3-dipolar cycloaddition reactions of 4,6-diazaphenanthrene 6-phenacylide formed in situ from the quaternary 6-phenacylbenzo[f][1,7]naphthyridinium bromide in basic medium were examined; methacrylic acid, methyl methacrylate, butyl vinyl ether, methyl vinyl ketone, maleic anhydride and dimethyl acetylenedicarboxylate were used as the dipolarophiles.
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10

Haas, Joseph E., and Donald A. Trego. "A Field Application of Hydrogen-Releasing Compound (HRCTM) for the Enhanced Bioremediation of Methyl Tertiary Butyl Ether (MTBE)." Soil and Sediment Contamination: An International Journal 10, no. 5 (September 2001): 555–75. http://dx.doi.org/10.1080/20015891109437.

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11

Guisado, I. M., J. Purswani, J. Gonzalez-Lopez, and C. Pozo. "Physiological and genetic screening methods for the isolation of methyl tert-butyl ether-degrading bacteria for bioremediation purposes." International Biodeterioration & Biodegradation 97 (January 2015): 67–74. http://dx.doi.org/10.1016/j.ibiod.2014.11.008.

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12

Nikpay, A., H. Kazemian, and M. Sadeghi. "Remediation of Methyl Tert-Butyl Ether Contaminated Water by Using In situ Catalytic and Biological Combined Techniques." American Journal of Environmental Sciences 4, no. 6 (June 1, 2008): 710–15. http://dx.doi.org/10.3844/ajessp.2008.710.715.

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13

Kane, S. R., H. R. Beller, T. C. Legler, C. J. Koester, H. C. Pinkart, R. U. Halden, and A. M. Happel. "Aerobic Biodegradation of Methyltert-Butyl Ether by Aquifer Bacteria from Leaking Underground Storage Tank Sites." Applied and Environmental Microbiology 67, no. 12 (December 1, 2001): 5824–29. http://dx.doi.org/10.1128/aem.67.12.5824-5829.2001.

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ABSTRACT The potential for aerobic methyl tert-butyl ether (MTBE) degradation was investigated with microcosms containing aquifer sediment and groundwater from four MTBE-contaminated sites characterized by oxygen-limited in situ conditions. MTBE depletion was observed for sediments from two sites (e.g., 4.5 mg/liter degraded in 15 days after a 4-day lag period), whereas no consumption of MTBE was observed for sediments from the other sites after 75 days. For sediments in which MTBE was consumed, 43 to 54% of added [U-14C]MTBE was mineralized to14CO2. Molecular phylogenetic analyses of these sediments indicated the enrichment of species closely related to a known MTBE-degrading bacterium, strain PM1. At only one site, the presence of water-soluble gasoline components significantly inhibited MTBE degradation and led to a more pronounced accumulation of the metabolite tert-butyl alcohol. Overall, these results suggest that the effects of oxygen and water-soluble gasoline components on in situ MTBE degradation will vary from site to site and that phylogenetic analysis may be a promising predictor of MTBE biodegradation potential.
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14

Hunger, M., T. Horvath, and J. Weitkamp. "Methyl tertiary-butyl ether synthesis on zeolite HBeta investigated by in situ MAS NMR spectroscopy under continuous-flow conditions." Microporous and Mesoporous Materials 22, no. 1-3 (June 1998): 357–67. http://dx.doi.org/10.1016/s1387-1811(98)00078-x.

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15

Hristova, Krassimira, Binyam Gebreyesus, Douglas Mackay, and Kate M. Scow. "Naturally Occurring Bacteria Similar to the Methyl tert-Butyl Ether (MTBE)-Degrading Strain PM1 Are Present in MTBE-Contaminated Groundwater." Applied and Environmental Microbiology 69, no. 5 (May 2003): 2616–23. http://dx.doi.org/10.1128/aem.69.5.2616-2623.2003.

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ABSTRACT Methyl tert-butyl ether (MTBE) is a widespread groundwater contaminant that does not respond well to conventional treatment technologies. Growing evidence indicates that microbial communities indigenous to groundwater can degrade MTBE under aerobic and anaerobic conditions. Although pure cultures of microorganisms able to degrade or cometabolize MTBE have been reported, to date the specific organisms responsible for MTBE degradation in various field studies have not be identified. We report that DNA sequences almost identical (99% homology) to those of strain PM1, originally isolated from a biofilter in southern California, are naturally occurring in an MTBE-polluted aquifer in Vandenberg Air Force Base (VAFB), Lompoc, California. Cell densities of native PM1 (measured by TaqMan quantitative PCR) in VAFB groundwater samples ranged from below the detection limit (in anaerobic sites) to 103 to 104 cells/ml (in oxygen-amended sites). In groundwater from anaerobic or aerobic sites incubated in microcosms spiked with 10 μg of MTBE/liter, densities of native PM1 increased to approximately 105 cells/ml. Native PM1 densities also increased during incubation of VAFB sediments during MTBE degradation. In controlled field plots amended with oxygen, artificially increasing the MTBE concentration was followed by an increase in the in situ native PM1 cell density. This is the first reported relationship between in situ MTBE biodegradation and densities of MTBE-degrading bacteria by quantitative molecular methods.
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16

Rodeghero, Elisa, Luisa Pasti, Elena Sarti, Giuseppe Cruciani, Roberto Bagatin, and Annalisa Martucci. "Temperature-Induced Desorption of Methyl tert-Butyl Ether Confined on ZSM-5: An In Situ Synchrotron XRD Powder Diffraction Study." Minerals 7, no. 3 (February 28, 2017): 34. http://dx.doi.org/10.3390/min7030034.

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17

Somsamak, Piyapawn, Hans H. Richnow, and Max M. Häggblom. "Carbon Isotope Fractionation during Anaerobic Degradation of Methyl tert-Butyl Ether under Sulfate-Reducing and Methanogenic Conditions." Applied and Environmental Microbiology 72, no. 2 (February 2006): 1157–63. http://dx.doi.org/10.1128/aem.72.2.1157-1163.2006.

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ABSTRACT Methyl tert-butyl ether (MTBE), an octane enhancer and a fuel oxygenate in reformulated gasoline, has received increasing public attention after it was detected as a major contaminant of water resources. Although several techniques have been developed to remediate MTBE-contaminated sites, the fate of MTBE is mainly dependent upon natural degradation processes. Compound-specific stable isotope analysis has been proposed as a tool to distinguish the loss of MTBE due to biodegradation from other physical processes. Although MTBE is highly recalcitrant, anaerobic degradation has been demonstrated under different anoxic conditions and may be an important process. To accurately assess in situ MTBE degradation through carbon isotope analysis, carbon isotope fractionation during MTBE degradation by different cultures under different electron-accepting conditions needs to be investigated. In this study, carbon isotope fractionation during MTBE degradation under sulfate-reducing and methanogenic conditions was studied in anaerobic cultures enriched from two different sediments. Significant enrichment of 13C in residual MTBE during anaerobic biotransformation was observed under both sulfate-reducing and methanogenic conditions. The isotopic enrichment factors (ε) estimated for each enrichment were almost identical (−13.4 to −14.6; r 2 = 0.89 to 0.99). A ε value of −14.4 ± 0.7 was obtained from regression analysis (r 2 = 0.97, n = 55, 95% confidence interval), when all data from our MTBE-transforming anaerobic cultures were combined. The similar magnitude of carbon isotope fractionation in all enrichments regardless of culture or electron-accepting condition suggests that the terminal electron-accepting process may not significantly affect carbon isotope fractionation during anaerobic MTBE degradation.
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18

Saponaro, Sabrina, Marco Negri, Elena Sezenna, Luca Bonomo, and Claudia Sorlini. "Groundwater remediation by an in situ biobarrier: A bench scale feasibility test for methyl tert-butyl ether and other gasoline compounds." Journal of Hazardous Materials 167, no. 1-3 (August 15, 2009): 545–52. http://dx.doi.org/10.1016/j.jhazmat.2009.01.026.

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19

Shi, Weijia, and Gang Zou. "Palladium-Catalyzed Room Temperature Acylative Cross-Coupling of Activated Amides with Trialkylboranes." Molecules 23, no. 10 (September 20, 2018): 2412. http://dx.doi.org/10.3390/molecules23102412.

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A highly efficient acylative cross-coupling of trialkylboranes with activated amides has been effected at room temperature to give the corresponding alkyl ketones in good to excellent yields by using 1,3-bis(2,6-diisopropyl)phenylimidazolylidene and 3-chloropyridine co-supported palladium chloride, the PEPPSI catalyst, in the presence of K2CO3 in methyl tert-butyl ether. The scope and limitations of the protocol were investigated, showing good tolerance of acyl, cyano, and ester functional groups in the amide counterpart while halo group competed via the classical Suzuki coupling. The trialkylboranes generated in situ by hydroboration of olefins with BH3 or 9-BBN performed similarly to those separately prepared, making this protocol more practical.
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20

Pongkua, Waleeporn, Rujira Dolphen, and Paitip Thiravetyan. "Bioremediation of gaseous methyl tert-butyl ether by combination of sulfuric acid modified bagasse activated carbon-bone biochar beads and Acinetobacter indicus screened from petroleum contaminated soil." Chemosphere 239 (January 2020): 124724. http://dx.doi.org/10.1016/j.chemosphere.2019.124724.

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21

Hosseini, Mehdi, and Nasser Dalali. "Use of Ionic Liquids for Trace Analysis of Methyl Tert-Butyl Ether in Water Samples using in situ Solvent Formation Microextraction Technique and Determination by GC/FID." Separation Science and Technology 49, no. 12 (August 12, 2014): 1889–94. http://dx.doi.org/10.1080/01496395.2014.894524.

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22

Talvenmäki, Harri, Niina Lallukka, Suvi Survo, and Martin Romantschuk. "Fenton’s reaction-based chemical oxidation in suboptimal conditions can lead to mobilization of oil hydrocarbons but also contribute to the total removal of volatile compounds." Environmental Science and Pollution Research 26, no. 33 (October 26, 2019): 34670–84. http://dx.doi.org/10.1007/s11356-019-06547-3.

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Abstract Fenton’s reaction-based chemical oxidation is in principle a method that can be utilized for all organic fuel residues thus making it a potential all-purpose, multi-contaminant, in situ application for cases in which storage and distribution of different types of fuels have resulted in contamination of soil or groundwater. Since peroxide breakdown reactions are also expected to lead to a physical transport of the target compound, this secondary physical removal, or rebound concentrations related to it, is prone to be affected by the chemical properties of the target compound. Also, since soil conditions are seldom optimal for Fenton’s reaction, the balance between chemical oxidation and transport may vary. In this study, it was found that, with a high enough hydrogen peroxide concentration (5 M), methyl tert-butyl ether–spiked groundwater could be treated even under suboptimal conditions for chemical mineralization. In these cases, volatilization was not only contributing to the total removal but also leading to rebound effects similar to those associated with air sparging techniques. Likewise for diesel, temporal transport from soil to the aqueous phase was found to lead to false positives that outweighed the actual remediation effect through chemical mineralization.
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23

Alpmann, Alexander, and Gertrud Morlock. "Rapid and Cost-Effective Determination of Acrylamide in Coffee by Planar Chromatography and Fluorescence Detection After Derivatization with Dansulfinic Acid." Journal of AOAC INTERNATIONAL 92, no. 3 (May 1, 2009): 725–29. http://dx.doi.org/10.1093/jaoac/92.3.725.

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Abstract A new method has been developed for the determination of acrylamide in ground coffee by planar chromatography using prechromatographic in situ derivatization with dansulfinic acid. After pressurized fluid extraction of acrylamide from the coffee samples, the extracts were passed through activated carbon and concentrated. These extracts were applied onto a silica gel 60 HPTLC plate and oversprayed with dansulfinic acid. By heating the plate, acrylamide was derivatized into the fluorescent product dansylpropanamide. The chromatographic separation with ethyl acetatetert.-butyl methyl ether (8 + 2, v/v) mobile phase was followed by densitometric quantification at 254/>400 nm using a 4 point calibration via the standard addition method over the whole system for which acrylamide was added at different concentrations at the beginning of the extraction process. The method was validated for commercial coffee. The linearity over the whole procedure showed determination coefficients between 0.9995 and 0.9825 (n = 6). Limit of quantitation at a signal-to-noise ratio of 10 was determined to be 48 g/kg. The within-run precision (relative standard deviation, n = 6) of the chromatographic method was 3. Commercial coffee samples analyzed showed acrylamide contents between 52 and 191 g/kg, which was in correlation with amounts reported in previous publications.
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24

Horvath, Thomas, Michael Seiler, and Michael Hunger. "A comparative study of methyl-tert-butyl ether synthesis on zeolites HY, HBeta, HBeta/F and HZSM-5 by in situ MAS NMR spectroscopy under flow conditions and on-line gas chromatography." Applied Catalysis A: General 193, no. 1-2 (February 2000): 227–36. http://dx.doi.org/10.1016/s0926-860x(99)00432-9.

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