Journal articles on the topic 'C-HOBIC'

AbstractIntroduction. Sarcoidosis is a rare, multi-organ disease of unknown etiology, characterized by the formation of granulomas mainly in the respiratory system.Aim. The aim of the study is to present a plan of care for patients with sarcoidosis, including International Classification for Nursing Practice – ICNP® terminology.Material and methods. The study was conducted in April 2019 at the Internal Disease Unit of the 7th Polish Navy Hospital in Gdansk, and included a 54-year-old patient hospitalized for sarcoidosis. The research employed the case study method and the technique of the interview, observation, analysis of medical records and measurements. The C-HOBIC data set was used to assess the patient’s condition.Results and conclusions. The reasons for the patient’s admission to the hospital were a continued raised temperature, erythema, as well as joint pain. The assessment and analysis of a patient’s status allowed us to formulate seven nursing diagnoses characteristic for sarcoidosis, which included: arthritis pain, functional dyspnoea, fever, impaired active range of motion, fatigue, risk for medication side effect and lack of knowledge of disease and 2 diagnoses resulting from the lifestyle of the patient: tobacco abuse and overweight. The process of nursing care planning included interventions fundamental for the above-mentioned diagnoses. They concerned the monitoring of symptoms and health indices, the patient’s knowledge, as well as the role of the nurse in the therapeutic process. The ICNP dictionary includes a sufficient range of terms, which allows the creation of a satisfactory nursing care plan for a patient with sarcoidosis.

Extracellular nucleotides exert an important role in controlling cell physiology by activating intracellular signalling cascades. Osteoblast HOBIT cells express P2Y1 and P2Y2 G-protein-coupled receptors, and respond to extracellular ATP by increasing cytosolic calcium concentrations. Early growth response protein 1 (Egr-1) is a C2H2-zinc-finger-containing transcriptional regulator responsible for the activation of several genes involved in the control of cell proliferation and apoptosis, and is thought to have a central role in osteoblast biology. We show that ATP treatment of HOBIT cells increases Egr-1 protein levels and binding activity via a mechanism involving a Ca2+-independent protein kinase C isoform. Moreover, hypotonic stress and increased medium turbulence, by inducing ATP release, result in a similar effect on Egr-1. Increased levels of Egr-1 protein expression and activity are achieved at very early times after stimulation (5 min), possibly accounting for a rapid way for changing the osteoblast gene-expression profile. A target gene for Egr-1 that is fundamental in osteoblast physiology, COL1A2, is up-regulated by ATP stimulation of HOBIT cells in a timescale that is compatible with that of Egr-1 activation.

Dalam peningkatan produksi hortikultura, khususnya sayuran di Indonesia selama ini masih menggunakan sistem pertanian konvensional dengan masukan input luar. Semakin tinggi biaya produksi terhadap pertumbuhan tanaman budidaya, maka akan mengakibatkan semakin tinggi pula masukan input luar seperti pestisida dan pupuk yang diberikan terhadap tanaman.Banyak orang yang menanam kangkung hanya untuk sekedar dikonsumsi sendiri dan melepas hobi bercocok tanam. Namun dalam bercocok tanam sering kali yang menjadikan masalah adalah lahan yang dimiliki tidak dapat ditanami tanaman sesukanya. Jika ingin menanam tanaman, maka dibutuhkan lahan tanah yang cukup luas. Daerah perkotaan saat ini sudah jarang memiliki lahan yang luas yang dapat digunakan sebagai media bercocok tanam. Akan tetapi saat ini banyak berbagai cara yang dapat dilakukan untuk sekedar meluapkan hobi bertanam, salah satunya adalah dengan budidaya kangkung dalam polybag dengan tambahan sekam padi bekas ayam petelur. Polybag adalah menanam tanaman ke dalam kantong plastik hitam. Cara ini dapat digunakan untuk membudidayakan tanaman kangkung, selain tidak membutuhkan lahan yang luas cara ini juga sangat mudah dan hemat untuk dilakukan. Tujuannya adalah untuk meningkatkan hasil tanaman kangkung darat ( Ipomoea reptans) dilakukan untuk mengetahui pengaruh sekam padi yang berasal dari kotoran ayam terhadap pertumbuhan dan produksi kangkung darat (Ipomoea reptans), menggunakan bahan dalam berbudidayanya seperti benih kangkung ( Ipomoea reptans), dan sekam padi yang berasal dari kotoran ayam. Budidaya tanaman kangkung merupakan budidaya tanaman yang menggunakan tahapan-tahapan budidaya seperti langkah awal adalah sediakan media tanam dengan menggunakna polybag,masukan tanah dan campuran sekam padi bekas kotoran ayam ke dalam polybag, usahakan untuk memberikan pupuk organik sehingga tanaman yang akan dihasilkan akan terbebas dari bahan kimia yang berbahaya, pilihlah bibit kangkung yang unggul dengan kualitas yang baik dan tidak busuk atau cacat. Penyemaian bibit kangkung dilakukan ke dalam polybag. Setelah bibit kangkung mulai tumbuh hingga terdapat 3 sampai 5 helai daun lalu pindahkan ke dalam polybag yang sudah disiapkan. Dalam satu polybag dapat menampung sekitar 3 sampai 5 batang kangkung untuk mendapatkan nutrisi yang cukup dan juga dapat tumbuh dengan baik. Lakukan penyiraman dalam waktu 2 kali dalam sehari agar tanah tidak kering dan juga tidak terlalu lembab. Hasil analisis usaha kangkung darat memiliki nilai keuntungan yaitu berdasarkan perhitungan R/C ratio yaitu diperoleh 2,59 dengan menggunakan sekam padi bekas kotoran ayam petelur dan yang tidak menggunakan sekam padi ( control) diperoleh R/C ratio yaitu 1,71. Jadi usaha kangkung darat, setiap Rp. 1,- biaya yang dikeluarkan akan mendapatkan penerimaan sebesar Rp. 2,59 (perlakuan dengan menggunakan sekam padi bekas kotoran aya) dan Rp. 1,71 ( pada perlakuan control).

Perpanjangan Pembatasan Sosial Berskala Besar (PSBB) selama pandemi COVID-19 menyebabkan pembatasan kegiatan, bisnis, sekolah, serta mobilitas sosial. Hal ini menjadi faktor tidak langsung penyebab masalah dan komplikasi COVID-19 pada remaja di usianya yang masih rentan antara lain dikarenakan anxietas, stres dan imunitas tubuh. Pengabdian masyarakat ini dilakukan melalui webinar secara terbuka pada 41 remaja di Desa Cilangkap Kabupaten Lebak dan peserta lain yang tersebar di Provinsi Banten untuk memberdayakan remaja melalui pemberian informasi manajemen stres dan informasi vitamin C untuk mengurangi risiko stres dan meningkatkan imunitas tubuh. Metode pelaksanaan dilakukan dengan metode kaji tindak (action research), partisipatif (pemberian informasi, metode konsultasi, advokasi, pendampingan secara daring/Zoom Meeting), serta monitoring dan evaluasi pelaksanaan kegiatan. Hasil kegiatan diantaranya terlaksananya pengabdian masyarakat dengan pemberian informasi manajemen stres dengan sasaran remaja yang berpotensi mengalami stress, terciptanya kreasi materi video tentang manajemen stress dan vitamin C, serta HAKI. Hasil evaluasi didapatkan dengan dilakukan analisa selama proses dan kuesioner setelah webinar. Peserta menyatakan menjadi tahu informasi tentang manajemen stres, materi video sangat menarik, seru, relevan, mantap, informasi sangat jelas, sangat membantu, sangat bermanfaat, 100% peserta menyatakan baik, sangat baik, sangat memuaskan, sangat senang, dan pada narasumber (sekaligus pengabdi), dapat menambah informasi, pengetahuan, pengalaman dan relasi. Kesimpulannya, pengabmas ini sangat menarik dan bermanfaat karena topik relevan dengan yang dialami dan dirasakan peserta selama pandemi. Saran peserta, diharapkan adanya seminar lanjutan dan dilaksanakan secara luring, sehingga dapat langsung memfasilitasi pada peningkatan pemberdayaan remaja yang kreatif dan produktif atas hobi/bakat/dan minat remaja, serta munculnya inovasi baru yang menarik dan berprofit.

Previous work from our group (Ravasio A, Hobi N, Bertocchi C, Jesacher A, Dietl P, Haller T. Am J Physiol Cell Physiol 300: C1456–C1465, 2011.) showed that contact of alveolar epithelial type II cells with an air-liquid interface (IAL) leads to a paradoxical situation. It is a potential threat that can cause cell injury, but also a Ca2+-dependent stimulus for surfactant secretion. Both events can be explained by the impact of interfacial tensile forces on cellular structures. Here, the strength of this mechanical stimulus became also apparent in microarray studies by a rapid and significant change on the transcriptional level. Cells challenged with an IAL in two different ways showed activation/inactivation of cellular pathways involved in stress response and defense, and a detailed Pubmatrix search identified genes associated with several lung diseases and injuries. Altogether, they suggest a close relationship of interfacial stress sensation with current models in alveolar micromechanics. Further similarities between IAL and cell stretch were found with respect to the underlying signaling events. The source of Ca2+ was extracellular, and the transmembrane Ca2+ entry pathway suggests the involvement of a mechanosensitive channel. We conclude that alveolar type II cells, due to their location and morphology, are specific sensors of the IAL, but largely protected from interfacial stress by surfactant release.

The list of animal viruses has been frequently added of new members raising permanent concerns to virologists and veterinarians. The pathogenic potential and association with disease have been clearly demonstrated for some, but not for all of these emerging viruses. This review describes recent discoveries of animal viruses and their potential relevance for veterinary practice. Dogs were considered refractory to influenza viruses until 2004, when an influenza A virus subtype H3N8 was transmitted from horses and produced severe respiratory disease in racing greyhounds in Florida/USA. The novel virus, named canine influenza virus (CIV), is considered now a separate virus lineage and has spread among urban canine population in the USA. A new pestivirus (Flaviviridae), tentatively called HoBi-like pestivirus, was identified in 2004 in commercial fetal bovine serum from Brazil. Hobi-like viruses are genetically and antigenically related to bovine viral diarrhea virus (BVDV) and induce similar clinical manifestations. These novel viruses seem to be widespread in Brazilian herds and have also been detected in Southeast Asia and Europe. In 2011, a novel mosquito-borne orthobunyavirus, named Schmallenberg virus (SBV), was associated with fever, drop in milk production, abortion and newborn malformation in cattle and sheep in Germany. Subsequently, the virus disseminated over several European countries and currently represents a real treat for animal health. The origin of SBV is still a matter of debate but it may be a reassortant from previous known bunyaviruses Shamonda and Satuperi. Hepatitis E virus (HEV, family Hepeviridae) is a long known agent of human acute hepatitis and in 1997 was first identified in pigs. Current data indicates that swine HEV is spread worldwide, mainly associated with subclinical infection. Two of the four HEV genotypes are zoonotic and may be transmitted between swine and human by contaminated water and undercooked pork meat. The current distribution and impact of HEV infection in swine production are largely unknown. Avian gyrovirus type 2 (AGV2) is a newly described Gyrovirus, family Circoviridae, which was unexpectedly found in sera of poultry suspected to be infected with chicken anemia virus (CAV). AGV2 is closely related to CAV but displays sufficient genomic differences to be classified as a distinct species. AGV2 seems to be distributed in Brazil and also in other countries but its pathogenic role for chickens is still under investigation. Finally, the long time and intensive search for animal relatives of human hepatitis C virus (HCV) has led to the identification of novel hepaciviruses in dogs (canine hepacivirus [CHV]), horses (non-primate hepaciviruses [NPHV] or Theiler's disease associated virus [TDAV]) and rodents. For these, a clear and definitive association with disease is still lacking and only time and investigation will tell whether they are real disease agents or simple spectators.

11039 Background: To address the role of the local metastatic microenvironment in the antiestrogen (AE) resistance exhibited by disseminated breast cancer (BrCa), we assembled heterotypic in vitro three-dimensional (3D) tissue cultures comprised of estrogen receptor-positive (ER+) BrCa cells and non-malignant accessory cells from organs frequently targeted by metastatic disease, to model the composition and architecture of metastatic lesions. Methods: MCF7 cells expressing luciferase, cultured alone or with accessory cells, were exposed to dose-ranges of hydroxytamoxifen (4-OHT), fulvestrant or raloxifene. MCF7 cells were also injected s.c. in nude mice either alone or with HS-5 bone marrow stromal cells (BMSCs). Tumor spheroid viability or xenograft growth were measured by bioluminescence. Results: MCF7 response to AEs in 3D conditions (collagen type I and/or Matrigel) was marked by acinar differentiation of tumor spheroids that resembles normal breast epithelium. Co-culture of MCF7 and immortalized accessory cells from the bone (BMSCs; HOBIT and hFOB osteoblasts), brain (SVGp12 astrocytes), liver (THLE3 hepatocytes), and lung (MRC9 fibroblasts) in 3D (but not 2D) conditions, conferred resistance to 4-OHT, raloxifene, and fulvestrant at clinically relevant doses. Heterotypic xenografts (MCF7 + BMSCs) had reduced response to tamoxifen compared with monotypic xenografts (MCF7 alone). BMSCs induced in MCF7 cells downregulation of TGFβ2; upregulation of a transcriptional signature enriched for genes associated with high-grade tumors, including genes involved in interferon response, signaling through EGFR superfamily members, NFkB and other antiapoptotic pathways; and elevated c-Myc protein levels. Exogenous TGFβ2 or neutralizing antibodies against INFγ partially re-sensitized MCF7 cells to 4-OHT in co-cultures. Conclusions: Our results indicate that accessory cells representing the microenvironment of metastatic sites confer AE resistance to BrCa cells in 3D tissue cultures and xenograft models. The mechanisms of stroma-induced AE resistance may reveal new therapeutic targets for refractory BrCa patients with metastatic disease.

Salah satu hobi yang banyak diminati masyarakat dan juga bisa menghasilkan uang adalah memelihara ikan. Ikan yang umum dipelihara ada dua jenis yaitu ikan hias predator dan juga ikan hias. Dengan harga jual yang berbeda dengan ikan konsumsi, dimana ikan hias memiliki kualitas yang berbeda-beda walaupun memiliki jenis dan ukuran yang sama. Bagi penghobi ikan Palmas senegalus albino semakin unik ikan palmas senegalus albino yang dimiliki maka semakin besar rasa bangga yang dimiliki. Bagi kebanyakan penghobi ikan palmas senegalus albino, memelihara ikan tidak melulu tentang uang tetapi tentang kepuasan saat melihat ikan palmas senegalus albino tumbuh dan berkembang dengan baik dengan memperhatikan kondisi aquarium dan juga konsumsi ikan palmas senegalus albino secara teratur sesuai dengan kebutuhan ikan palmas senegalus albino. Tujuan dari penelitian ini untuk memudahkan pemberian pakan serta mematikan lampu aquarium sehingga meminimalisir kemungkinan ikan setres dan tanaman di aquarium mati. Penambahan antar muka di smartphone memudahkan para penghobi ikan untuk memberikan pakan pada ikan dan memonitoring lampu aquarium apakah menyala atau mati pada aplikasi Blynk pada smartphone penghobi ikan. Metode yang digunakan adalah dengan metode Waterfall dan metode perancangan dimana artinya adalah menggabungkan, penulis menganalisa kebutuhan dan melalui melalui metode Waterfall tersebut penulis merancang tahapan-tahapan yang akan dilakukan. Dengan menggunakan Arduino IDE sebagai lingkungan pengembangan program serta menggunakkan bahasa pemrograman C. Hasil penelitian yang dicapai adalah ikan Palmas senegalus albino mendapatkan porsi makan yang sesuai dengan kebutuhan serta tanaman yang berada di aquarium mendapatkan cahaya yang cukup sehingga tidak mati dan menjadi racun dan juga lumut tidak berkembang dengan cepat. Kesimpulannya adalah alat ini memberikan porsi pakan ikan palmas senegalus albino yang sesuai kebutuhan serta cahaya lampu 8 jam agar tanaman pada aquarium hidup dan berkembang dengan baik dan lumut tidak cepat menyebar.

Статтю присвячено вивченню впливу чинника статі мовного посередника на застосовувані ним пріоритетні стратегії під час створення цільового тексту. Емпіричним матеріалом дослідження слугували тексти англомовної художньої прози та їхні українські переклади у виконанні чоловіків та жінок. Застосований у праці психолінгвістичний підхід до аналізу двомовного корпусу дав змогу встановити деякі ‘S-універсалії’ (Chesterman, 2011) вибору різностатевими посередниками тих чи тих мовних структур, що дає підстави погодитися з іншими дослідниками в питанні існування значущих відмінностей у гендерних пріоритетах структурування перекладного дискурсу. Серед найпомітніших “жіночих” універсалій в перекладі українською – метакогнітивна гнучкість, що кваліфікуємо як синтетичний когнітивний стиль. Крім того, простежено застосування перекладачками стратегії статусної нейтралізації в діалозі між персонажами під час відтворення статусно-маркованих і дифузних ситуацій; вищі показники лексичного розмаїття, експліцитації й номіналізації, ніж у цільових версіях перекладачів чоловічої статі. Останні виявляють більшу обережність у перекладі, вдаючись до ‘семантичного методу’ (Newmark, 1988) перекладу, виявляючи відтак аналітичний когнітивний стиль поведінки, а також демонструючи стратегію негативної ввічливості під час відтворення діалогів персонажів у статусно-маркованих і статусно-дифузних комунікативних ситуаціях. Література References Бендас Т. В. Гендерная психология. – СПб.: Питер, 2007.Bendas, T. (2007). Gendernaya Psikhologiya [Gender Psychology]. St. Petersburg: Piter. Карасик В. И. Социальный статус человека в лингвистическом аспекте // “Я”, “Субъект”,“Индивид” в парадигмах современного языкознания. – М. : ИЯ РАН, 1992. – С. 47–74.Karasik, V. I. (1992). 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Buah pepaya (Carica papaya L.) tergolong buah yang populer dan digemari oleh hampir seluruh masyarakat di dunia. Pepaya banyak dibudidayakan oleh masyarakat baik sebagai hobi ataupun usaha komersil. Hal ini karena pepaya memiliki banyak kelebihan. Buah pepaya menjadi salah satu komoditi yang sangat mudah rusak, hal tersebut terjadi akibat beberapa faktor, seperti: aktivitas dan pertumbuhan mikroba pada buah pepaya, aktivitas enzim-enzim di dalam buah pepaya, perlakuan suhu, serta beberapa faktor lainnya yang dapat merusak buah pepaya. Dehidrasi osmosis merupakan teknik pengurangan kadar air yang dapat digunakan untuk mengatasi permasalahan tersebut yaitu dengan cara perendaman bahan pada larutan berkonsentrasi tinggi. Penelitian ini dilaksanakan pada bulan September 2018 hingga bulan Oktober 2018 di Laboratorium Pengolahan Pangan dan Analisis Kimia Universitas Hasanuddin. Tujuan dari penelitian ini yaitu untuk mengetahui pengaruh suhu dan konsentrasi larutan gula terhadap kadar air akhir, penurunan bobot (WR), jumlah padatan yang masuk (SG) dan jumlah air yang keluar dari bahan (WL) pada pepaya selama proses dehidrasi osmosis dan mengetahui suhu dan konsentrasi yang optimal dalam proses dehidrasi pepaya. Metode yang digunakan pada penelitian ini adalah dengan menggunakan rancangan acak lengkap yang disusun secara faktorial dengan 2 faktor dan masing-masing faktor terdiri atas 3 level yaitu kadar gula 40 Brix, 50 Brix, 60 Brix dan suhu perendaman 40 C, 50 C, 60 C dengan 3 kali ulangan. Berdasarkan hasil penelitian diperoleh bahwa perlakuan suhu 60 dan konsentrasi larutan 60 Brix menghasilkan kadar air yang paling rendah, dan sama halnya seperti peningkatan nilai Solid Gain (SG) paling tinggi juga terjadi pada suhu 60 C dan konsentrasi 60 Brix. Perlakuan suhu perendaman 50 C dan konsentrasi larutan gula 50 Brix menghasilkan Weight Reduction (WR) yang paling tinggi, sedangkan nilai Water Loss (WL) yang paling tinggi juga berada pada kombinasi perlakuan suhu perendaman 50 C dan konsentrasi larutan gula 50 Brix.

HoBi-like pestivirus (HoBiPeV) has been reported in several biological samples from cattle worldwide, but there are no descriptions of this virus associated with neurological symptoms. This report described the first occurrence of neurological disease associated with HoBiPeV in a newborn dairy calf. A mixed-breed Holstein calf had severe neurological symptoms at birth and died at 21 days old. The tissue fragments (central nervous system (CNS), myocardium, liver, kidney, lung, intestine, and spleen) were submitted to reverse transcription (RT)–PCR assay for the partial 5'-untranslated region (5'UTR) and N-terminal autoprotease (Npro) gene of the pestivirus genome, and the CNS tissue fragments were submitted to histopathological and immunohistochemical evaluation. The RT–PCR assay indicated that the kidney, CNS, and intestinal tissue fragments were positive for the pestivirus 5'UTR, and the CNS and intestinal tissue fragments were positive for the pestivirus Npro gene. Amplicons with high DNA quantification in the 5'UTR (CNS—cerebral cortex) and Npro (CNS—cerebral cortex and intestine) RT–PCR assays were sequenced. The nucleotide (nt) sequence and phylogenetic analysis of the 5'UTR strain exhibited 93.6 to 99.4%, 85%, 89.4 to 89.9%, 85.1%, and 90.5 to 91.5% nt identity with HoBiPeV strains from clades a, b, c, d, and e, respectively. The Npro amplicons showed 99.7% nt identity to each other and 90.4 to 96.5%, 85.1 to 85.3%, 79.2 to 79.7%, and 85.8 to 86.5% nt identity with HoBiPeV strains from clades a, c, d, and e, respectively. A histopathology revealed neuronal necrosis at the cerebrum, cerebellum, and brain stem. An immunohistochemical assay designed to identify antigens of bovine viral diarrhea virus revealed positive intracytoplasmic immunoreactivity within neurons at the cerebral cortex, cerebrum, cerebellum, and spinal cord. Thus, this report provides information about the first identification of HoBiPeV in tissues of the CNS in a newborn dairy calf with neurological symptoms.

Pestiviruses are a common causative agent of gastrointestinal infections in calves. Viruses infect a wide range of domestic and wild animals, cause pathology of the gastrointestinal tract, respiratory, reproductive organs, and the immune system. According to the modern classification, the genotype of pestiviruses is designated by the letters of the Latin alphabet: A, B, C, etc. Common pestivirus infections include viral diarrhea-mucosal disease (VD-MD). The causative agent is pestivirus of genotypes A and B. The virus of genotype A is represented 20 subtypes (1a-1t) and three subtypes (2a-2c). The type A virus in newborn calves causes diarrhea with profuse watery discharge, dehydration. Infection of the type B virus is characterized by a malignant course of the disease in the form of fever, hemorrhagic inflammation of the gastrointestinal tract, and high mortality. In 2004, pestivirus infection of small ruminants was registered for the first time in several countries, caused by an atypical pestivirus, designated as Hobi virus, type D. respiratory organs in calves. Common pathogens include bovine coronavirus (cattle) from the Coronaviridae family, order Nidovirales, subgroup 2a. There are three forms of cattle coronavirus infection: diarrhea of newborn calves; winter diarrhea with the hemorrhagic syndrome in cows and transport fever. A disease similar in symptomatology is caused by rotaviruses. According to the antigenic properties of the main proteins, rotaviruses are divided into groups, subgroups, and serotypes. The main pathogens for calves are group A rotaviruses. Rotavirus diarrhea occurs in newborn calves in the first three weeks. Modern immunological, molecular genetic methods have made it possible to identify new enteropathogenic cattle viruses. Among them, norovirus, the causative agent of a highly contagious anthropozoonotic disease with a fecal-oral transmission mechanism, belongs to the Caliciviridae family, the Norovirus genus, genogroups III and V. Some viruses that cause gastrointestinal diseases are isolated both from sick calves and from animals without visible signs of the disease. Other pathogens are Nebovirus, a non-enveloped virus, a member of the Caliciviridae family, Cattle Torovirus, an enveloped virus of the Coronaviridae family, Astroviruses, a non-enveloped virus of the Astroviridae family. The family consists of the genera: Mamastrovirus mammals and Avastrovirus birds. Cattle picornaviruses, represented by kobuvirus (also called aichivirus B), belongs to the genus Enterovirus in the Picornaviridae family, found in the feces of calves with diarrhea. At the same time, other components of the microbiome of the gastrointestinal tract of calves: bacteria, fungi, rickettsiae - complicate viral infections or, on the contrary, have antiviral properties.