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Journal articles on the topic 'Caco2 and HaCaT'

Author: Grafiati

Published: 4 June 2021

Last updated: 1 February 2022

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1

Lin, Kuen-Tze, Tsu-Chung Chang, Feng-Yi Lai, Chun-Shu Lin, Hsing-Lung Chao та Shih-Yu Lee. "Rhodiola crenulata Attenuates γ-Ray Induced Cellular Injury via Modulation of Oxidative Stress in Human Skin Cells". American Journal of Chinese Medicine 46, № 01 (2018): 175–90. http://dx.doi.org/10.1142/s0192415x18500106.

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Skin injury is a major complication during radiation therapy and is associated with oxidative damage to skin cells. An effective and safe radioprotectant to prevent this skin damage is still unavailable. The Rhodiola crenulata root extract (RCE) has been reported to be a free radical scavenger and a potent anti-oxidant in both in vitro and in vivo models. In the current study, we investigated the effects of RCE on ionizing radiation-induced skin injury and its underlying mechanisms. HaCaT cells — a non-cancerous skin cell line together with HepG2, Caco2, A549, and OECM cancer cell lines — were

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2

Mitropoulou, Gregoria, Marianthi Sidira, Myria Skitsa, et al. "Assessment of the Antimicrobial, Antioxidant, and Antiproliferative Potential of Sideritis raeseri subps. raeseri Essential Oil." Foods 9, no. 7 (2020): 860. http://dx.doi.org/10.3390/foods9070860.

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The aim of the present study was to investigate the antimicrobial potential of Sideritis raeseri subps. raeseri essential oil (EO) against common food spoilage and pathogenic microorganisms and evaluate its antioxidant and antiproliferative activity. The EO was isolated by steam distillation and analyzed by GC/MS. The main constituents identified were geranyl-p-cymene (25.08%), geranyl-γ-terpinene (15.17%), and geranyl-linalool (14.04%). Initially, its activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Listeria monocytogenes, Salmonella Enteritidis, Salmonell

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3

Lishchynskyi, Ostap, Yurij Stetsyshyn, Joanna Raczkowska, et al. "Fabrication and Impact of Fouling-Reducing Temperature-Responsive POEGMA Coatings with Embedded CaCO3 Nanoparticles on Different Cell Lines." Materials 14, no. 6 (2021): 1417. http://dx.doi.org/10.3390/ma14061417.

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In the present work, we have successfully prepared and characterized novel nanocomposite material exhibiting temperature-dependent surface wettability changes, based on grafted brush coatings of non-fouling poly(di(ethylene glycol)methyl ether methacrylate) (POEGMA) with the embedded CaCO3 nanoparticles. Grafted polymer brushes attached to the glass surface were prepared in a three-step process using atom transfer radical polymerization (ATRP). Subsequently, uniform CaCO3 nanoparticles (NPs) embedded in POEGMA-grafted brush coatings were synthesized using biomineralized precipitation from solu

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4

Barndt, Robert B., Mon-Juan Lee, Nanxi Huang, et al. "Targeted HAI-2 deletion causes excessive proteolysis with prolonged active prostasin and depletion of HAI-1 monomer in intestinal but not epidermal epithelial cells." Human Molecular Genetics 30, no. 19 (2021): 1833–50. http://dx.doi.org/10.1093/hmg/ddab150.

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Abstract Mutations of SPINT2, the gene encoding the integral membrane, Kunitz-type serine inhibitor HAI-2, primarily affect the intestine, while sparing many other HAI-2-expressing tissues, causing sodium loss in patients with syndromic congenital sodium diarrhea. The membrane-bound serine protease prostasin was previously identified as a HAI-2 target protease in intestinal tissues but not in the skin. In both tissues, the highly related inhibitor HAI-1 is, however, the default inhibitor for prostasin and the type 2 transmembrane serine protease matriptase. This cell-type selective functional

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5

Gandamalla, Durgaiah, Harikiran Lingabathula, and Narsimha Reddy Yellu. "CYTOTOXICITY EVALUATION OF TITANIUM AND ZINC OXIDE NANOPARTICLES ON HUMAN CELL LINES." International Journal of Pharmacy and Pharmaceutical Sciences 9, no. 10 (2017): 240. http://dx.doi.org/10.22159/ijpps.2017v9i11.21924.

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Objective: In vitro cytotoxicity evaluation of titanium dioxide, 20 nm (TNP 20) and zinc oxide, 20 nm (ZNP 20) nanoparticles (NP) were tested on different types of human skin (HaCat), lung (A549), liver (Hep G2) and colon (Caco-2) cell cultures in relevance to human risk assessmentMethods: The different concentrations of test TNP 20 and ZNP 20 1-300 µg/ml were exposed to determine the cell viability reduction on four human cell lines after 48 h post exposure using 3-(4, 5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT). The mitochondrial membrane activities of the viable cells we

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6

Andreani, Tatiana, Verónica Nogueira, Ana Gavina, et al. "Ecotoxicity to Freshwater Organisms and Cytotoxicity of Nanomaterials: Are We Generating Sufficient Data for Their Risk Assessment?" Nanomaterials 11, no. 1 (2020): 66. http://dx.doi.org/10.3390/nano11010066.

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The aim of the present study was to investigate the eco-cytotoxicity of several forms of nanomaterials (NM), such as nano-CuO, nano-TiO2, nano-SiO2 and nano-ZnO, on different aquatic species (Raphidocelis subcapitata, Daphnia magna and Lemna minor) following standard protocols and on human cell lines (Caco-2, SV-80, HepG2 and HaCaT). Predicted no-effect concentrations (PNEC) or hazard concentrations for 5% of the species (HC5) were also estimated based on the compilation of data available in the literature. Most of the NM agglomerated strongly in the selected culture media. For the ecotoxicity

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7

Almutary, Abdulmajeed G., and Barbara J. S. Sanderson. "Toxicity of N-Nitrosodimethylamine and SiO2 nanoparticles to HaCaT and caco-2 cell lines found in waste water treatment." Journal of Toxicology and Health 4, no. 1 (2017): 4. http://dx.doi.org/10.7243/2056-3779-4-4.

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8

Dursun, Feyza Nur, and Erdinc Bal. "Hasat Sonrası Bazı Uygulamaların Autumn Giant Erik Çeşidinin Muhafaza Performansı Üzerine Etkisi." Turkish Journal of Agriculture - Food Science and Technology 8, no. 2 (2020): 504. http://dx.doi.org/10.24925/turjaf.v8i2.504-510.3070.

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In this study, the effect of postharvest salicylic acid (2 mM), oxalic acid (5 mM), putrescine (2 mM) and calcium (4% CaCl2) applications on storage performance of Autumn Giant plum fruit were investigated. After applications, plum fruits were placed in modified atmosphere packaging (MAP) and stored for 40 days at 85-90% relative humidity conditions at 0.5±0.5°C. In order to determine the fruit quality characteristics after treatments, weight loss, soluble solids content, titratable acidity, fruit firmness, ascorbic acid, total flavonoids, total phenolics and total antioxidant content analysis

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9

Luckanagul, Jittima Amie, Pahweenvaj Ratnatilaka Na Bhuket, Chawanphat Muangnoi, Pranee Rojsitthisak, Qian Wang, and Pornchai Rojsitthisak. "Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities." Polymers 13, no. 2 (2021): 194. http://dx.doi.org/10.3390/polym13020194.

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A hyaluronic acid-grafted poly(N-isopropylacrylamide) (HA-pNIPAM) was synthesized as a polymeric nanogel platform for encapsulation and delivery of hydrophobic bioactive compounds using curcumin as a model drug. As demonstrated by transmission electron microscopy and dynamic light scattering techniques, the HA-pNIPAM was simply assembled into spherical nano-sized particles with the thermoresponsive behavior. The success of curcumin aqueous solubilization was confirmed by fluorescent spectroscopy. The resulting nanogel formulation enhanced the aqueous solubility and uptake into NIH-3T3 cells of

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10

Luckanagul, Jittima Amie, Pahweenvaj Ratnatilaka Na Bhuket, Chawanphat Muangnoi, Pranee Rojsitthisak, Qian Wang, and Pornchai Rojsitthisak. "Self-Assembled Thermoresponsive Nanogel from Grafted Hyaluronic Acid as a Biocompatible Delivery Platform for Curcumin with Enhanced Drug Loading and Biological Activities." Polymers 13, no. 2 (2021): 194. http://dx.doi.org/10.3390/polym13020194.

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A hyaluronic acid-grafted poly(N-isopropylacrylamide) (HA-pNIPAM) was synthesized as a polymeric nanogel platform for encapsulation and delivery of hydrophobic bioactive compounds using curcumin as a model drug. As demonstrated by transmission electron microscopy and dynamic light scattering techniques, the HA-pNIPAM was simply assembled into spherical nano-sized particles with the thermoresponsive behavior. The success of curcumin aqueous solubilization was confirmed by fluorescent spectroscopy. The resulting nanogel formulation enhanced the aqueous solubility and uptake into NIH-3T3 cells of

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11

Hoang, Thi Xoan, Cao Nguyen Duong, and Jae Young Kim. "Identification and Characterization of a Splicing Variant in the 5′ UTR of the Human TLR5 Gene." BioMed Research International 2017 (2017): 1–7. http://dx.doi.org/10.1155/2017/8727434.

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Toll-like receptors (TLRs) are essential components of the innate immune system. TLR5 is the receptor for flagellin, the principal protein component of bacterial flagella. The TLR5 gene has 6 exons. In an RT-PCR analysis, we found long TLR5 transcripts, in addition to those of the expected size (short TLR5 transcripts). A sequence analysis revealed that the long TLR5 transcripts contain a new exon of 94 nucleotides located between previously reported exons IV and V in the 5′ untranslated region (5′ UTR). A real-time PCR analysis of the two alternatively spliced variants in various cell lines s

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12

Ramos, Patrícia A. B., Catarina Moreirinha, Sara Silva, et al. "The Health-Promoting Potential of Salix spp. Bark Polar Extracts: Key Insights on Phenolic Composition and In Vitro Bioactivity and Biocompatibility." Antioxidants 8, no. 12 (2019): 609. http://dx.doi.org/10.3390/antiox8120609.

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Salix spp. have been exploited for energy generation, along with folk medicine use of bark extracts for antipyretic and analgesic benefits. Bark phenolic components, rather than salicin, have demonstrated interesting bioactivities, which may ensure the sustainable bioprospection of Salix bark. Therefore, this study highlights the detailed phenolic characterization, as well as the in vitro antioxidant, anti-hypertensive, Staphylococcus aureus growth inhibitory effects, and biocompatibility of Salix atrocinerea Brot., Salix fragilis L., and Salix viminalis L. bark polar extracts. Fifteen phenoli

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13

Lameirão, Fátima, Diana Pinto, Elsa F. Vieira, et al. "Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro." Antioxidants 9, no. 3 (2020): 267. http://dx.doi.org/10.3390/antiox9030267.

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Chestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4–46 min) and temperature (34–76 °C) in the antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total

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14

Santos-Rebelo, Ana, Catarina Garcia, Carla Eleutério, et al. "Development of Parvifloron D-loaded Smart Nanoparticles to Target Pancreatic Cancer." Pharmaceutics 10, no. 4 (2018): 216. http://dx.doi.org/10.3390/pharmaceutics10040216.

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Pancreatic cancer is the eighth leading cause of cancer death worldwide. For this reason, the development of more effective therapies is a major concern for the scientific community. Accordingly, plants belonging to Plectranthus genus and their isolated compounds, such as Parvifloron D, were found to have cytotoxic and antiproliferative activities. However, Parvifloron D is a very low water-soluble compound. Thus, nanotechnology can be a promising delivery system to enhance drug solubility and targeted delivery. The extraction of Parvifloron D from P. ecklonii was optimized through an acetone

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15

Yuyun, Yonelian, Pahweenvaj Ratnatilaka Na Bhuket, Wiwat Supasena, et al. "A Novel Curcumin-Mycophenolic Acid Conjugate Inhibited Hyperproliferation of Tumor Necrosis Factor-Alpha-Induced Human Keratinocyte Cells." Pharmaceutics 13, no. 7 (2021): 956. http://dx.doi.org/10.3390/pharmaceutics13070956.

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Curcumin (CUR) has been used as adjuvant therapy for therapeutic application in the treatment of psoriasis through several mechanisms of action. Due to the poor oral bioavailability of CUR, several approaches have been developed to overcome the limitations of CUR, including the prodrug strategy. In this study, CUR was esterified with mycophenolic acid (MPA) as a novel conjugate prodrug. The MPA-CUR conjugate was structurally elucidated using FT-IR, 1H-NMR, 13C-NMR, and MS techniques. Bioavailable fractions (BFs) across Caco-2 cells of CUR, MPA, and MPA-CUR were collected for further biological

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16

Sljivic Husejnovic, Maida, Martina Bergant, Sasa Jankovic, et al. "Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)." Environmental Geochemistry and Health 40, no. 4 (2018): 1557–72. http://dx.doi.org/10.1007/s10653-018-0071-6.

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17

Takayama, Yoshiharu, Reiji Aoki, Ryo Uchida, Atsushi Tajima, and Ayako Aoki-Yoshida. "Role of CXC chemokine receptor type 4 as a lactoferrin receptor." Biochemistry and Cell Biology 95, no. 1 (2017): 57–63. http://dx.doi.org/10.1139/bcb-2016-0039.

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Lactoferrin exerts its biological activities by interacting with receptors on target cells, including LDL receptor-related protein-1 (LRP-1/CD91), intelectin-1 (omentin-1), and Toll-like receptor 4 (TLR4). However, the effects mediated by these receptors are not sufficient to fully explain the many functions of lactoferrin. C-X-C-motif cytokine receptor 4 (CXCR4) is a ubiquitously expressed G-protein coupled receptor for stromal cell-derived factor-1 (SDF-1/CXCL12). Lactoferrin was found to be as capable as SDF-1 in blocking infection by an HIV variant that uses CXCR4 as a co-receptor (X4-trop

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18

Radzimierska-Kaźmierczak, Marta, Krzysztof Śmigielski, Magdalena Sikora, et al. "Olive Oil with Ozone-Modified Properties and Its Application." Molecules 26, no. 11 (2021): 3074. http://dx.doi.org/10.3390/molecules26113074.

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Olive oil application in the cosmetic industry may be extended by its ozonation, bringing about new oil properties and increased stability. Olive oil treated with 0.04 mole O3 or 0.10 mole O3 per 100 g oil was subjected to chemical parameters evaluation and composition scrutinizing by gas chromatography–mass spectrometry (GC-MS) and headspace solid-phase microextraction (HS-SPME) GC-MS analysis. The biological activity of refined and ozonated oil included their antimicrobial properties by the agar diffusion method and cytotoxicity by the MTT assay towards two normal (LLC-PK1, HaCaT) and two ca

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19

Geiger, Julian, Rebecca Doelker, Sofia Salö, Thomas Roitsch, and Louise T. Dalgaard. "Physiological phenotyping of mammalian cell lines by enzymatic activity fingerprinting of key carbohydrate metabolic enzymes: a pilot and feasibility study." BMC Research Notes 12, no. 1 (2019). http://dx.doi.org/10.1186/s13104-019-4697-y.

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Abstract Objective Enzymatic fingerprinting of key enzymes of glucose metabolism is a valuable analysis tool in cell physiological phenotyping of plant samples. Yet, a similar approach for mammalian cell line samples is missing. In this study, we applied semi-high throughput enzyme activity assays that were originally designed for plant samples and tested their feasibility in extracts of six frequently used mammalian cell lines (Caco2, HaCaT, C2C12, HEK293, HepG2 and INS-1E). Results Enzyme activities for aldolase, hexokinase, glucose-6-phosphate dehydrogenase, phosphoglucoisomerase, phosphogl

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20

Ieiri, Hiromi, Natsuki Kameda, Junko Naito, et al. "Paramylon extracted from Euglena gracilis EOD-1 augmented the expression of SIRT1." Cytotechnology, September 15, 2021. http://dx.doi.org/10.1007/s10616-021-00494-z.

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AbstractEuglena gracilis, a type of microalgae, contains several nutrients and accumulates paramylon, a β-1,3-glucan. In recent studies, paramylon has shown to exhibit various activities including immunomoduratory and hepatoprotective effects. In the present study, using an in vitro cell culture system, we aimed to determine whether paramylon derived from the E. gracilis EOD-1 strain, which produces large amounts of paramylon, can augment SIRT1 expression in epidermal cells via activating gut–skin interactions. Results showed that paramylon augmented the expression of SIRT1 in Caco-2 cells, a

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Hake, Alexander, Nico Symma, Stefan Esch, Andreas Hensel, and Martina Düfer. "Alkaloids from Lime Flower (Tiliae flos) Exert Spasmodic Activity on Murine Airway Smooth Muscle Involving Acetylcholinesterase." Planta Medica, July 16, 2021. http://dx.doi.org/10.1055/a-1534-3766.

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AbstractLime flower (Tiliae flos) is traditionally used either for treatment of the common cold or to relieve symptoms of mental stress. Recently, the presence of a new class of piperidine and dihydro-pyrrole alkaloids from lime flower has been described. The present study aimed to investigate the pharmacological activity of hydroacetonic lime flower extracts, alkaloid-enriched lime flower fractions, and isolated alkaloids on the murine airway smooth muscle and the cholinergic system. While a hydroacetonic lime flower extract did not show any pharmacological activity, enriched Tilia alkaloid f

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