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Journal articles on the topic 'Cafe culture'
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Pangestu, Felina Angelia, and Winda Halim. "Coffee Shop Re-Design For Low-Vision Baristas and Visitors (Case Study at Café X Bandung)." RSF Conference Series: Engineering and Technology 2, no. 1 (April 22, 2022): 43–52. http://dx.doi.org/10.31098/cset.v2i1.534.
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Café X is a coffee shop managed by 3 low-vision baristas. Visitors to this cafe are also dominated by visitors with visual impairments. Due to the limited visibility of the barista and some cafe visitors, several problems were found that could cause discomfort for workers and cafe visitors. Therefore, it is necessary to adjust the cafe's operating system to support performance and provide security and comfort to the baristas and visitors who come. The data collected are data on raw materials, machinery, and equipment for cafe operational activities, data on physical facilities, layout and actual physical environment of cafes, data on the application of 5S around the cafe area, as well as data on potential accidents that may arise while around the cafe area. Then the data will processed using the method of comparison with anthropometric data and design concepts in improvement for physical facilities and cafe layouts. Standardization comparisons to improve physical environment. Radar maps to determine the application of 5S culture. Fish bones and 5W+1H to describe the causes of potential accidents in the cafe area. The suggestions given are the adjustment of the machines and equipment used, redesign of the physical facilities and layout of the cafe, the proposed design of tools in the form of tactile stickers and the application of guiding blocks as directions, improvement of the physical environment, application of 5S culture to maintain the work area and provide suggestions prevention of accidents in café area.
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Hayati, Rezkina, Iiz Izmuddin, and Anne Putri. "Pengaruh Religiusitas Dan Budaya Terhadap Prilaku Konsumen Muslim Dalam Membeli Makanan Kafe." EKONOMIKA SYARIAH : Journal of Economic Studies 3, no. 1 (October 24, 2019): 72. http://dx.doi.org/10.30983/es.v3i1.1265.
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<em>This research attempts to analyze the effect of religiosity and culture on purchasing decisions in buying cafe food in Bukittinggi partially or simultaneously. The design of this study is quantitative correlational. The population is Muslim teenagers in the city of Bukittinggi. The sample of this study was Muslim teenagers in the Bukit Tinggi town who bought cafe food. Furthermore, the determination of the sample to be used as the respondent was done by purposive sampling, by taking and selecting Muslim adolescents in Bukittinggi as respondents.Based on the results of the study it can be concluded that: partially Religiosity and Culture have a positive effect on purchasing decisions in buying cafe food, while Religiosity and Culture simultaneously influence purchasing decisions in buying cafe food where the contribution of the determination coefficient (R Square) is 38.8% and the remaining 61.2% is influenced by other variables which are not included in this similarity regression or variables not examined.</em>
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Hatami, Farshad, and Avideh Talaei. "The Explanation of The Indices and Components of The Cafe in The Traditional and Modern Times of Iran." Indonesian Journal for Social Responsibility 3, no. 2 (2021): 95–112. http://dx.doi.org/10.36782/ijsr.v3i02.76.
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Several factors affecting the cultural growth of the society are the correct function of social interaction, daily activities in the workplace, and increasing social action according to population growth rate need to be more evident. Review of past history showed the arrival of cafe culture in the Safavid era and Shah Abbas(I) and establishment of coffee houses in Qajar and Pahlavi era with social, economic and cultural function. In the first Pahlavi period, with the arrival of a new style of cafe from the west, the social approach and architectural style of the coffee house changed to a coffee shop. The young people of the society, as the audience, chose the coffee shop as a platform for social communication. In this article, we try to explain the course of physical and cultural developments of cafes and increase the benefit of young people from temporary urban social exchange centres as a third place (home, workplace and coffee shop) by interpreting the study examples of traditional and modern cafes in the period. We will pay attention to the Pahlavi, contemporary and modern West.
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Okta Wibowo, Tangguh, Wening Udasmoro, and Ratna Noviani. "Understanding New Consumption Sites of Internet Cafe in Yogyakarta, Indonesia." Jurnal Ilmu Sosial dan Ilmu Politik 23, no. 3 (May 21, 2020): 237. http://dx.doi.org/10.22146/jsp.51707.
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In the Indonesian context, we did not find any previous study on internet cafes that discussed the transformation from warnet to internet cafe. Based on our study in Yogyakarta, we found a new internet cafe style that we did not find in other cities. This study offers a new understanding of analyzing internet cafes that users not only consume a computer to access the internet, but they “consume” space as well. This study aims to explore that transformation and to reveal the expansion of consumption patterns. This study emphasizes the empirical investigation to gather the data. We conducted qualitative in-depth interviews and observations from January to June 2018. We also examined several previous studies about internet cafes to expose a gap that can be extended in this study. Based on findings, we found that the transformation from warnet was a way to survive amid mobile technology. This transformation gives an implication to the new means of consumption. However, the expansion of consumption patterns can be explored from the consumption of space and digital content. Overall, digital content is the new insight to understand that internet cafes are not only a place to copy contents, but this can be seen as a symbol of empowerment to local users. Therefore, internet cafes can be seen as media-industry bottom lines to promote a culture of resistance to digital content without going to the store or going to the cinema.
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Yang, Min. "The Construction of IC Service and University Library’s Café." Advanced Materials Research 452-453 (January 2012): 872–75. http://dx.doi.org/10.4028/www.scientific.net/amr.452-453.872.
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With the information era’s coming, readers’ requirements and behaviors have changed, so the university library need to innovate traditional ways of management and service. The establishment of cafe in the library caters to the reading requirements of readers; it is a one-stop service including the learning support, culture and leisure, intercourse and collaboration. The background of university library’s cafe is analyzed briefly; its functions and constructing elements are explained in detail. The university library cafe will be the information acquisition, knowledge learning and cooperation exchange place which has comfortable environment, improved equipments and centralized functions.
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Duncan, Rebecca, Jabina Coleman, Sharon Herring, Meg Kawan, Christy Santoro, Meghana Atre, Aleigha Mason, Shawana Moore, and Aparna Kumar. "Breastfeeding Awareness and Empowerment (BAE): A Black Women-Led Approach to Promoting a Multigenerational Culture of Health." Societies 12, no. 1 (February 18, 2022): 28. http://dx.doi.org/10.3390/soc12010028.
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(1) Background: Critical gaps in the U.S. healthcare system perpetuate adverse reproductive health outcomes for Black people. Grounded in reproductive justice and trauma-informed care, Breastfeeding Awareness and Empowerment (BAE) has developed a program titled BAE Cafe to directly address these gaps by providing community-based lactation and perinatal mental health support. A literature review identified key programmatic gaps, namely, access to knowledge relevant to troubleshooting breastfeeding, peer support, community support and healthcare system support, and system-level factors that impede families and communities from accessing lactation support. (2) Methods: This paper describes BAE Cafe through a group process observation and participant survey. (3) Results: The observation of groups highlighted the core elements of the BAE Cafe model: knowledge, support and mental health support in a peer driven format. Participant survey feedback was overwhelmingly positive and highlighted the critical importance of lactation support for Black women by Black women and BAE’s role in participants’ decisions to continue breastfeeding. (4) Conclusions: BAE Cafe is a replicable, scalable, peer-driven and low-barrier intervention that has the potential to improve outcomes for Black families. Additional research and investment are now needed to assess large-scale implementation to reduce disparities and address health inequity across different contexts and settings.
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Buss, Andrew, and Jason Lardy. "A New Community Space: The Culture of the Urban Bike Cafe." Spaces and Flows: An International Journal of Urban and ExtraUrban Studies 5, no. 3-4 (2015): 13–25. http://dx.doi.org/10.18848/2154-8676/cgp/v05i3-4/53692.
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Mo, Heejune, and Junghwa Kim. "A study on the development direction of cultural tourism contents using cultural navigation: Focusing on Maps of Coffee Shops and Cafes Appearing in Modern Literature." Barun Academy of History 11 (June 30, 2022): 37–57. http://dx.doi.org/10.55793/jkhc.2022.11.37.
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This study intends to discuss the direction of building a cultural navigation system that can enjoy cultural tourism contents by mixing navigation systems within the basic framework of maps. In particular, the goal is to trace the location of Gyeongseong's coffee shops and cafes appearing in modern literature, apply them to their current location, examine them in terms of cultural heritage, and further develop them into the field of cultural tourism content using them.
Coffee shops and cafes are one of the most important items in examining the culture of modern Gyeongseong, and have been studied in various ways in modern literature and cultural history. The roles of coffee shops and cafes have changed somewhat in modern times, but writers and artists of modern Gyeongseong were enthusiastic about coffee shops and cafes, the symbols of modern culture at the time. Numerous coffee shops and cafes have been opened, and the writers have described them in various media or novels.
In this study, the location of coffee shops and cafes that appeared in modern Gyeongseong is substituted into the current location, and through cultural navigation, the location of the past and present, information on coffee shops and cafes, and how the coffee shops or cafes are expressed in literature. By providing a system that researches and introduces information on whether or not information has been made, it is intended to examine the cultural meaning of the contemporary coffee shop and cafe culture, and to provide its cultural value by restoring it in the form of a digital map.
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Ahmad, Harun, Endang Sumarti, and Yunita Anas Sriwulandari. "Cafe as a Representation of the Lifestyle of the Urban Community." Technium Social Sciences Journal 33 (July 9, 2022): 569–76. http://dx.doi.org/10.47577/tssj.v33i1.6851.
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Café is one of the contemporary products that has become an arena of lifestyle battles. Supported by a culture of consumerism and commodity fetishism, the Café then transformed into a lifestyle representation of urban society. There are many phenomena that support this thesis. Those who come to the Café are not only to fulfill their basic needs (the need to drink coffee), but more than that, which is to show others their existence with all the success they have achieved. Drinking coffee in a café with the aim of imaging is the core of the culture of consumerism and commodity fetishism, which Anthony Giddens (1991:198) calls a human identity project.
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Dinda Hermawan, Theresa, and Arido Laksono. "Family Business Organizational Culture and Its Implications for Human Resources Success in the Legendary Milk Café Karangdoro Semarang." E3S Web of Conferences 359 (2022): 02030. http://dx.doi.org/10.1051/e3sconf/202235902030.
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This study focuses on family organizational culture and its implications for the succession of human resources in the legendary milk cafe Karangdoro Semarang. This research is motivated by quite a number of failures experienced by businesses to family companies in Indonesia. Many of these failures can be a reflection of poor resource management in family companies. According to Schein, individual performance in organizations cannot be understood without taking into account organizational culture, because organizations determine the degree of organizational effectiveness. From this statement, understanding the organizational culture of family companies is very important, because there are many examples of how difficult it is for a family business or company in Indonesia to maintain its survival from generation to generation. Human resources are an important aspect of business, especially in a family business. The data in this study were obtained through in-depth interviews with several owners and visitors of the milk café Karangdoro Semarang. From this research, it can be seen that the milk cafe Karangdoro Semarang has a culture in which all employees and managers are members of their own family that has been running for up to 4 generations.
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Baruno, Agustiawan, and Juli Ani Muliya Abady. "THE INFLUENCE OF MARKETING MIX FACTORS AND COMMUNITY CULTURE ON THE BEHAVIOR OF CONSUMER BUYING INTEREST IN THE VARIETY OF ICED COFFEE MILK AT JANJI JIWA." Ekspektra : Jurnal Bisnis dan Manajemen 6, no. 1 (July 1, 2022): 46–57. http://dx.doi.org/10.25139/ekt.v6i1.4663.
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This study aims to analyze the influence of several marketing mix factors such as product, price, location, promotion, and community culture on purchasing decisions for the Iced Coffee Milk product variant at the Janji Jiwa Cafe. The population of this research is the visitors of the Janji Jiwa Cafe Volume 65 Surabaya. Samples were taken using random sampling technique. Samples were taken as many as 100 loyal buyers of Iced Coffee Milk at the Janji Jiwa Cafe Volume 65 Surabaya who were met during the distribution of the questionnaire. Data obtained from the distribution of questionnaires. The data analysis technique used multiple linear regression and hypothesis testing using F-test and t-test. Based on the F-test, it is known that simultaneously product, price, location, promotion, and culture have a significant effect on purchasing decisions because the significance value is less than 0.05, which is 0.000. Based on the t-test, it is known that the product has a significant effect on purchasing decisions because the significance value is smaller than 0.05, which is 0.002. Price has a significant effect on purchasing decisions because the significance value is smaller than 0.05, which is 0.007. Location has a significant effect on purchasing decisions because the significance value is smaller than 0.05, which is 0.005. Promotion has a significant effect on purchasing decisions because the significance value is smaller than 0.05, which is 0.004. Meanwhile, culture has a significant effect on purchasing decisions because the significance value is smaller than 0.05, which is 0.001.
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Supriyoso, Patria, and Dede Supriadi. "Positioning Strategy for Excelso Coffee Buyers At Mayfair Bandung." Economic: Journal Economic and Business 1, no. 1 (October 3, 2022): 34–40. http://dx.doi.org/10.56495/ejeb.v1i1.245.
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The activity of drinking coffee is only intended to relieve drowsiness, but has also become part of a modern lifestyle, changes in culture, environment and technology make coffee not only considered as an ordinary drink, but has become an icon of self-identity and a symbol of socialization. . In addition, the majority of Indonesian people are coffee fans. Excelso comes with a coffee cafe where executives and professionals hang out with segmentation, targeting and positioning, bringing hangout places and places designed to be as comfortable as possible so that they feel at home in Excleso cafe, with drinks and food that are all coffee. In addition, Excelso's innovation is the presence of coffee from all over Indonesia with various flavors and coffee grounds with packaging that can be brewed at home as well as services and a comfortable place for Excelso buyers so that they can promote to their colleagues and friends to come to the Excelso cafe.
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Rohmawati, Iis. "FORMULASI STRATEGI PEMASARAN MENGGUNAKAN ANALISA QSPM (STUDI PADA SUOKLAT CAFE DI SURABAYA)." Aplikasi Administrasi: Media Analisa Masalah Administrasi 20, no. 2 (March 22, 2018): 114. http://dx.doi.org/10.30649/aamama.v20i2.85.
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The aim of this research is: (1) Identifying and analyzing internal and external environment of the company that influence the marketing strategy of Suoklat Cafe. (2) Formulating the alternative marketing strategy of Suoklat Cafe that is obtained from the analysis result. (3) Formulating and setting the strategy formula that is most suitable to improve Suoklat Cafe. The data collection is taken from April to August 2016 in Suoklat Cafe located in Surabaya.Internal Analysis (IFE) results the primary factor focusing on chocolate products with the score 0,172. Meanwhile, the main weakness from this business is the less appropriate management implementation process scoring 0, 117. When the two internal factors are combined, the average total score is 2, 635.External Analysis (EFE) results main opportunity that can be used by Suoklat Cafe in order to face the competition; hanging out culture in Cafe in Surabaya by 0,216. The main threat that can be obtained from the calculation shows through the strong factor of bargaining high supplier, scoring 0, 212. Based on the two external factors that is obtained, the average score is 2, 916. IE Analysis in Suoklat Cafe positions it in quadrant V, known as maintaining and preserving strategy. SWOT analysis results strategies that can be applied: (1) Maintaining the price, increasing the variation and the product quality in order to enchance the loyality and consumers' satisfactions, (2) Expanding the market share, (3) Maintaining and improving the service quality towards the consumers (4) improving the internal managemeny of the company (5) streghten the capital through cooperations with the media, the investors, and the governmeny agencies (6) analyzing the competitors to find out tje advantages and the disadvantages, and also streghten the consumer-supplier relationship to help increase the loyality (7) Improving the effeciency and effectiveness of the employee's work, expanding the location of the parking area or cooperating with local authorities, also increasing the technology. The QSPM processing results the production of main prior strategy that sets market share expansion to face the competition with TAS 6, 422. Keyword :Strategy formulation, Marketing, SWOT, QSPM.
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Hidayati, Rika, and Ikhwan Ikhwan. "Perilaku Gaya Hidup Hedonisme di Kalangan Mahasiswa Kurang Mampu Fakultas Ilmu Sosial UNP." Culture & Society: Journal Of Anthropological Research 1, no. 1 (September 6, 2019): 38–45. http://dx.doi.org/10.24036/culture/vol1-iss1/6.
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Riset ini dilatarbelakangi oleh ketertarikan peneliti dalam melihat maraknya perilaku gaya hidup hedonisme di kalangan mahasiswa kurang mampu di Fakultas Ilmu Sosial UNP. Riset ini memiliki tujuan untuk memahami bagaimana perilaku hedonisme di kalangan mahasiswa kurang mampu FIS UNP. Riset ini di kaji menggunakan teori masyarakat konsumsi dari Jean Baudrillard. Baudrillard berasumsi bahwa masyarakat mengkonsumsi suatu barang atau jasa bukan lagi dilihat pada pemenuhan kebutuhan (nneds), tetapi lebih kepada pemenuhan hasrat (desire). Metode yang dipakai dalam riset ini adalah pendekatan kualitatif tipe deskriptif, teknik pemilihan informan snowball sampling dan jumlah informan sebanyak 10 orang. Pengumpulan data dibuat dengan teknik observasi, wawancara, dan pengumpulan dokumen dan dikaji menggunakan teknik analsis data dari Miles dan Huberman. Hasil riset ini menyatakan Perilaku Gaya Hidup Hedonisme Di Kalangan Mahasisswa Kurang Mampu FIS UNP ditunjukkan melalui kegiatan-kegiatan sebagai berikut: (1) shopping; (2) Nongkrong di cafe; (3) nonton bioskop; (4) karaoke; (5) jalan-jalan/refreshing.
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Budarina, Kateryna, Larysa Sharan, Natalia Bondar, and Vyacheslav Gubenya. "EXPEDIENCY OF INTRODUCING A CRAFT CAVE IN A HOSPITALITY INSTITUTION." GEOGRAPHY AND TOURISM, no. 60 (2020): 10–15. http://dx.doi.org/10.17721/2308-135x.2020.60.10-15.
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Goal. The purpose of the article is to substantiate the feasibility of introducing a craft cafe on the basis of a business hotel, in particular, introducing the local stage of raw raw materials preparation in the technology of preparing coffee beans. Indeed, according to the study, the provision of services for organizing the preparation of a coffee drink in hotels is not satisfactory. And the quality of raw materials leaves much to be desired. Methodology. The methodological basis of this work is the work of domestic and foreign scientists in the field of hospitality, scientific periodicals, Internet resources. The methodology includes generalizing and statistical data, which reflects the feasibility of introducing a preparatory operation for processing raw materials in the technology of making coffee precisely on the territory of a hospitality establishment. Results. As a result of the research, the international and domestic experience of introducing a craft cafe in a business hotel was analyzed. It was found that there is no such practice of implementation in domestic and foreign hospitality establishments. An algorithm was developed for the introduction of a craft cafe, ways of using secondary raw materials in additional services were proposed, and the positive effect of coffee on the human body was confirmed. The results of the implementation will help improve the culture of coffee consumption and expand its range, while providing the hospitality enterprise with the opportunity to develop and make significant profits. Scientific novelty. The necessity of introducing a craft cafe with its own roast on the territory of a hospitality enterprise has been proved. The world experience in the introduction of craft cafes in Ukraine has been systematized. The assortment of additional services that contribute to increasing the competitiveness of the institution, as well as which will enable business people during business trips to enjoy high-quality coffee drinks and maintain the beauty of their body, are presented. Practical significance. The materials presented in the work can be used to improve and expand the range of additional services provided in domestic hospitality enterprises: to expand the range of coffee beans of our own production with different degrees of roasting for the manufacture of various coffee drinks, to reduce costs when purchasing batches of finished beans at auctions, while increasing their competitiveness not only in Ukraine, but also far beyond its borders.
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Wahyuningtyas, Dianka, Farah Levyta, and Maria Pia Adiati. "TRAINING FOR MAKING KALANG BEVERAGE USING REED ROOTS FOR EMPOWERMENT OF PKK MOTHERS IN CAFE JLAMPRANG KELURAHAN KRAPYAK, PEKALONGAN." ICCD 2, no. 1 (November 25, 2019): 26–32. http://dx.doi.org/10.33068/iccd.vol2.iss1.166.
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Pekalongan is one of the tourism destinations in Indonesia. Some cultures of Pekalongan can be developed and promoted especially in the culinary field. Pekalongan's special food and beverage offerings are one of the most potential products in attracting both local and foreign tourists. Not only because of its unique name but also because of the process of making and philosophy in a product that makes Pekalongan culinary specialties. But on its journey, Pekalongan cuisine has not been able to make the reason tourists come to visit Pekalongan. Akar alang-alang which is one of the special traditional drink ingredients of Pekalongan which is expected to be better known and available at Café Jlamprang, Krapyak Lor Village so that it can introduce Pekalongan's culinary culture more easily to the community and can embrace the young generation to get to know the Pekalongan culture. Because what is currently available are some drinks that use root-alang alang ingredients that can only be found in certain regions or in the form of instant drinks that can be found in souvenirs. In this study shows that Kalang has a unique taste and presentation. Then the production system can be done simply. Kalang has a high enough potential to be sold at Cafe Jlamprang, Krapyak Lor village so that if it is able to be developed and managed properly, efforts to preserve traditional drinks should be done not only by producers, but the government must also involve all elements of society.
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Moon, Geun-Yi, and Ho-Chang Lyu. "A Study on the Spatial Presentation Strategies for Cafe as 'The Third Space' - Focused on the cafes located in regions where 'Street culture' was formed -." Korean Institute of Interior Design Journal 22, no. 5 (October 31, 2013): 68–77. http://dx.doi.org/10.14774/jkiid.2013.22.5.068.
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Kubiński, Piotr. "Play It Again, Stanley: Mise en Abyme and Playing with Convention and Narrative in The Stanley Parable." Tekstualia 1, no. 3 (February 1, 2017): 45–59. http://dx.doi.org/10.5604/01.3001.0013.5929.
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Humanistic reflection on video games seems like a necessity nowadays, due to the enormous popularity of digital entertainment and the infl uence it exerts over modern culture. This need for a deeper refl ection also stems from the fact that, increasingly, video games give their players various pleasures – created by spectacular visual effects, exciting plot or an opportunity to solve logical puzzles. Electronic games are progressively becoming a tool of artistic pursuits and intellectual experiments that would be impossible in analogue media. An example of such phenomenon, and a game especially worthy of refl ection, is The Stanley Parable [Galactic Cafe, 2013]. The author of the article focuses on it.
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Çam, Ninel. "Stehblues–Do You Want to Dance with Me?" Nordic Journal of Dance 8, no. 1 (June 1, 2017): 16–25. http://dx.doi.org/10.2478/njd-2017-0003.
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Abstract The participatory dance performance STEHBLUES was created by the duo of Ninel Çam (an artist) and Chris Schaal (a filmmaker), who travel to different cities, mark a 2m x 2m area in a public space, and display an illustrated sign that lets passersby know that they can dance with the standing-and-waiting artist if they want. Chris Schaal documents the performances through still images and videos. Moving images are live-streamed to the World Cafe in Stuttgart. In STEHBLUES, the artist stands in the midst of the marked field in a public space, waiting until a stranger is willing to slow-dance with her. As soon as someone volunteers, a relationship is created in this sphere of dance and becomes an ephemeral part of the city, of the culture, of the world. STEHBLUES travels to different cities that have invited this project to be part of their culture or to cities with which the artists feel a connection.
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Imani, Yashifa Anindita, and Ratna Endah Santoso. "Perancangan Pelengkap Interior Dengan Inspirasi Interior Maroko (Studi Kasus Ruang VIP Marakez Cafe & Resto)." Ornamen 19, no. 2 (December 1, 2022): 160–69. http://dx.doi.org/10.33153/ornamen.v19i2.3827.
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Marakez Café & Resto is a restaurant that serves Middle Eastern specialties in Surakarta. Carrying the Café & Resto, Marakez has several spots and rooms, one of which is a VIP room provided for guests with facilities that can be considered exclusive compared to other rooms. The shape of this VIP room is actually very supportive, with a short sofa that allows customers to enjoy their food in a lesehan style, just like Middle Eastern culture, but unfortunately the interior elements in this room do not reflect the Moroccan feel as the name suggests. Based on the description that has been explained, it is necessary to design interior textile elements to build a Moroccan atmosphere because it has been supported by the shape of the room. This design uses the design method presented by Colin Clipson. The results of this design produce complementary interior styles that are based on ideas from the Moroccan interior, namely arch, zellij and mashrabiya. This design also combines two techniques in surface design, namely digital printing and embroidery, the product is an interior complement in the form of sofa upholstery, curtains and pillowcases.
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Hendarti, Religiana, Vivien Soebiyan, and Yanita Milla. "ANALYSIS OF THE APPLICATION OF BETAWI ARCHITECTURE IN CAFE DESIGN IN JAKARTAFROM THE ASPECT OF AFFORDABILITY IN CONSTRUCTION." ICCD 2, no. 1 (November 28, 2019): 562–67. http://dx.doi.org/10.33068/iccd.vol2.iss1.270.
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This paper presents a study of the things that determine the implementation of Betawi architecture in a 'café' design for a community in Jakarta. The café for this community is a project to facilitate the activities of PKK women in Palmerah District, Jakarta. The majority of these communities are local residents who are also mostly Betawi ethnic, therefore, this design requires an affordable design, in the sense of a design that can represent local culture with the application of Betawi architecture that can meet limited funding. As is known, Betawi architecture is architecture that is rich in detail ornamentation and made of wood. The result of this design is a 'café' design that uses the most important Betawi ornament with a frame structure and iron construction.
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Kleinman, Sharon S. "Cafe Culture in France and the United States: A Comparative Ethnographic Study of the Use of Mobile Information and Communication Technologies." Atlantic Journal of Communication 14, no. 4 (December 2006): 191–210. http://dx.doi.org/10.1207/s15456889ajc1404_1.
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ШАРИФУЛИН, Сергей, Sergej SHARIFULIN, Алина КУРАГИНА, and Alina KURAGINA. "THE SUCCESS FACTORS OF CONFECTIONERY BUSINESS." Services in Russia and abroad 11, no. 5 (August 7, 2017): 102–25. http://dx.doi.org/10.22412/1995-042x-11-5-9.
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The article is devoted to theoretical issues of success of confectionery business. It examines the factors affecting the process of economic activity of the confectionery enterprise. Sweets are a source of taste pleasure and positive emotions and for most consumers become a vital product. In the modern world a tea culture with sweets is widely developed, therefore confectionery business will always be quite promising. The business "with a sweet taste" has a lot of manifestations, but at the present time the tendency of development of such enterprises as cafe-confectioneries is obvious. Cafe-confectionery is a public catering enterprise that specializes in selling with consumption in its own accommodation a wide range of different types of confectionery products. The authors note, that in order to meet the client's expectations, the confectionery company should be able to navigate the market, know the wishes of consumers, effectively organize their production and serve the guests so that they return again. Modern confectionery business is riddled with service subtleties, special importance is the identification of the needs of its client. The need for sweets for modern people is becoming so natural that many people do not see their daily meals without confectionery. Each person satisfies the need for sweet, based on their preferences, as well as financial opportunities.
There are many success factors; complex interaction of these factors among themselves will ensure a high demand for products, and hence the success of the café-confectionery. The article highlights groups of factors: organization of production, customer service, marketing and management. The authors analyze each group in detail, and gives an appropriate description of each success factor of the confectionery business.
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Nur Ain Basri, Siti, Faridahanim Ahmad, Nur Izie Adiana Abidin, Ishak Baba, Hasnida Harun, Nor Hazren Abdul Hamid, Mariah Awang, et al. "Digital Campus." International Journal of Engineering & Technology 9, no. 2 (April 18, 2020): 382. http://dx.doi.org/10.14419/ijet.v9i2.30272.
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Mobile is everywhere, changing the way we work, play, socialize, and learn. Students nowadays are immersed in a digital culture driven by mobile consumer experiences across a range of devices, from wearable to phones, tablets and virtual-reality platforms. Digital Campus is a website application that is in University Tun Hussien Onn Pagoh Campus. The purpose of this website is to search all locations located in Pagoh Campus such as laboratory, admin office, dean office, cafe and classroom. Digital campus website is embedded with Open Street Map. It is open databased licensing and it is a collaborative mapping. This website is developed using Joomla 3.8.13 with PHP version, 5.6.25. There are 204 locations were plotted using this link https://digitalcampus.uthm.edu.my/index.php. Digital campus based on Open Street Map can helps students, admin staff, lectures and visitors to find location in very easy way by using digital platform. Thus, this application facilities can optimization of administration work and promote university management to higher performances.
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Balu, Febi Adriani, Ronald P. C. Fanggidae, and Paulina Yuritha Amtiran. "ANALISIS PENGEMBANGAN OBYEK WISATA PANTAI OESAPA DI KOTA KUPANG." Journal of Management : Small and Medium Enterprises (SMEs) 10, no. 3 (February 2, 2020): 327–45. http://dx.doi.org/10.35508/jom.v10i3.2000.
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This study aims to analyze the supporting and inhibiting factors for the development of Oesapa Beach attractions in Kupang City. This type of research is qualitative research using qualitative descriptive research methods. Data sources consist of primary data obtained through observation and interviews; and secondary data sources come from records, interview results and documents from relevant agencies in the study. The results of the study show that the factors that support the development of Oesapa Beach attractions are: (1) Tourist attraction, is anything that has a unique, easy, and value in the form of natural diversity, culture, and man-made results that are targeted or tourist visits; (2) Visitors, are someone who visits a tourist area with the aim of having fun and spending less time, energy and money in less than 24 hours. While the inhibiting factors for the development of Oesapa Beach attractions are: (1) Accessibility, is the degree of ease achieved by a person towards an object, service or environment; (2) Promotion is an effort to offer products or services with the aim of attracting consumers to buy or consume them; (3) Human Resources (HR), namely the workforce involved in tourism is still inadequate and professional; and (4) Funds, is one of the benchmarks for the progress of the development of sustainable tourism objects. Oesapa Beach has a tourist attraction in the form of beautiful and natural scenery, lopo-lopo lined on the beach, and lined with cafes with colorful tents and unique and different cafe designs. In addition to the tourist attraction, visitors who are increasingly busy visiting this tourist attraction become a supporting factor for tourism objects can be developed.
Keywords: Analysis, Obstacle Factors, Supporting Factors, Tourism
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Nizovkina, S. "THE TABLE AND ITS ROLE IN THE DEVELOPMENT OF PUBLIC SPACES." National Association of Scientists 4, no. 75 (February 1, 2022): 43–46. http://dx.doi.org/10.31618/nas.2413-5291.2022.4.75.566.
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The article is devoted to the concept of public space and its functionality during the study of coffee shops in Novosibirsk. The necessity for public space per person is an urgent topic, since every person somehow finds himself inside this space and assumes a special role in society. Semiotic, theoretical and empirical research methods were used in the study of the topic. Important aspects in the formation of modern urban public spaces are their functionality and complexity, which determines the components of this environment. Public spaces fulfill the task of broadcasting urban culture from generation to generation and have the ability to adapt to the rapidly changing needs of residents. An analysis of the gender, age and psychological characteristics of coffee shop visitors makes it possible to understand the reasons for visiting coffee shops, as well as the relevance of this public space in general. The article analyzes the role of tables in the social practices of society and the age composition of visitors to the cafe of the city. As a result of the study, it was found that furniture inside public spaces affects not only the aesthetic component, but also the change in social relationships between people.
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Amtha, Rahmi, and Ardianto Kurniadi. "An overview of oral mucosa condition of shisha smoker." Journal of Dentomaxillofacial Science 1, no. 2 (August 31, 2016): 214. http://dx.doi.org/10.22208/jdmfs.1.2.2016.214-219.
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Shisha is a water pipe that tobacco extract and fruit scented burnt using coal. It produces the smoke through the vessel and inhaled using a hose with good taste. The culture of shisha smoking is popular in Midle East country that curently has been also entering Indonesia. The side effect of shisha smoking habit is still very rare reported. Aim of this study is to describe the oral mucosa condition of shisha user. A preliminary observasional study was conducted at several sisha cafe at South Jakarta. Under informed consent, subject with habit of tobacco and shisha smoker were included. Sociodemographic data (age, gender, duration, frequency of smoking), salivary flow rate and oral mucosa changes were documented. Eighteen subjects were recruited into this study. Most of shisha smoker was also tobacco smoker. Shisha was more practiced by male at age (15-24 years old). The oral mucosa changes such as keratosis, melanosis, leukoedema, coated tongue, gingivitis and xerostomia were found on subject with habit of tobacco smoking habit only or both shisha and tobacco smoking. In conclusion apparently the shisha smoking habit may casue oral mucosa changes almost the same with tobacco smoking habit
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Amtha, Rahmi, and Ardianto Kurniadi. "An overview of oral mucosa condition of shisha smoker." Journal of Dentomaxillofacial Science 1, no. 2 (August 31, 2016): 80. http://dx.doi.org/10.15562/jdmfs.v1i2.1.
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Shisha is a water pipe that tobacco extract and fruit scented burnt using coal. It produces the smoke through the vessel and inhaled using a hose with good taste. The culture of shisha smoking is popular in Midle East country that curently has been also entering Indonesia. The side effect of shisha smoking habit is still very rare reported. Aim of this study is to describe the oral mucosa condition of shisha user. A preliminary observasional study was conducted at several sisha cafe at South Jakarta. Under informed consent, subject with habit of tobacco and shisha smoker were included. Sociodemographic data (age, gender, duration, frequency of smoking), salivary flow rate and oral mucosa changes were documented. Eighteen subjects were recruited into this study. Most of shisha smoker was also tobacco smoker. Shisha was more practiced by male at age (15-24 years old). The oral mucosa changes such as keratosis, melanosis, leukoedema, coated tongue, gingivitis and xerostomia were found on subject with habit of tobacco smoking habit only or both shisha and tobacco smoking. In conclusion apparently the shisha smoking habit may casue oral mucosa changes almost the same with tobacco smoking habit
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Rifauddin, Machsun, and Arfin Nurma Halida. "Konsep Desain Interior Perpustakaan untuk Menarik Minat Kunjung Pemustaka." Pustakaloka 10, no. 2 (December 31, 2018): 162. http://dx.doi.org/10.21154/pustakaloka.v10i2.1424.
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ABSTRAKTren masyarakat modern saat ini lebih senang membaca, belajar, dan berkumpul, sambil nongkrong ditempat-tempat hiburan dibanding harus pergi ke perpustakaan. Disisi lain, saat ini diberbagai pusat perbelanjaan seperti mall dan tempat wisata telah banyak didirikan public space dengan konsep mini library. Salah satu alasan masyarakat lebih memilih tempat tersebut dibanding perpustakaan adalah desain interior yang menarik dan aspek kenyamananya. Perpustakaan diharapkan mampu menyesuaikan diri dengan tren dan budaya masyarakat saat ini untuk menarik kembali minat pemustakanya. Penelitian ini bertujuan untuk menjelaskan konsep desain interior perpustakaan baik secara teoritis maupun praktis berdasarkan kajian literatur. Hasil penelitian menunjukkan bahwa desain interior merupakan bagian penting yang harus diperhatikan pengelola perpustakaan. Desain interior selain untuk menarik minat kunjung pemustaka juga dapat meningkatkan minat baca, membentuk citra positif perpustakaan, dan menumbuhkan kepuasan bagi pemustaka. Selain memperhatikan aspek estetika, desain interior sebuah perpustakaan tetap harus memperhatikan aspek fungsionalnya. Inovasi perpustakaan berkonsep Post-Modern Space dengan tambahan cafe dan lounge dapat diterapkan dalam perpustakaan modern dengan tetap memperhitungkan tata ruang, tata warna, pencahayaan, sirkulasi udara, dan tata suara.ABSTRACTModern society trends today prefer to read, study, and gather, while hanging out in places of entertainment rather than having to go to the library. On the other hand, currently in various shopping centers such as malls and tourist attractions have been established many public space with the concept of a mini library. One reason people prefer that place compared to the library is the attractive interior design and comfort aspects. Libraries are expected to adapt to the current trends and culture of society to reclaim the interests of its librarians. This study aims to explain the concept of library interior design both theoretically and practically based on literature review. The results showed that interior design is an important part that must be considered by library managers. Interior design in addition to attract visitors visit can also increase interest in reading, forming a positive image of the library, and foster satisfaction for pemustaka. In addition to considering the aesthetic aspect, the interior design of a library must still pay attention to its functional aspects. Post-Modern Space concept innovation with additional cafe and lounge can be applied in modern libraries while taking into account the layout, color, lighting, air circulation and sound system.
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Резник, Галина, Galina Reznik, Т. Соколкова, and T. Sokolkova. "Event-Marketing As a Key Tool of the Complex Progress in the Service Sector." Scientific Research and Development. Economics of the Firm 8, no. 2 (August 7, 2019): 10–18. http://dx.doi.org/10.12737/article_5d0c94b2659926.01566707.
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Introduction: the article deals with the essence of event-marketing as a popular tool for promotion in the market, the place of event-marketing in the complex of marketing communications is determined. A study on the development of the Park of culture and recreation “Olympic” based on the use of event-marketing tools is carried out. The results of the study of existing tools to promote the Park are given, the motives and preferences of potential guests of the Park on the basis of a survey are identified, recommendations for its development are given. Materials and methods: the article uses the publications of well-known scientists, methods of abstract-logical, systematic, economic and statistical, comparative analysis, graphic, methods of sociological and marketing research (in the survey of consumers of the Park of culture and recreation “Olympic”). The results of the study: the analysis of existing ways to promote the services of the Park of culture and recreation “Olympic” showed that the Park is not enough and not often held various event-events. In addition, the events are not diverse, which indicates the lack of use of event-marketing tools in the Park. In this regard, a variety of events was offered, within the framework of the implementation of such areas as sport events, exhibitions and art creativity, leisure activities for young people, romantic events, catering, opening and restoration of the existing Playground, modernization of the roller rink and art cafe, opening of the Park of extreme species, improvement of the Park and the opening of the school with clubs for the implementation of creative directions. Discussion and conclusion: Improvement program Park development using the tools of event marketing will contribute to the implementation of the Federal program “Formation of a comfortable urban environment for 2018–2022.g.”, to increase the customer traffic, to improve the visitor traffic, average check, increase the quality of life of the population. For organizations in the sphere of communication services, Event-marketing is the most effective direction of direct communication and is one of the most popular and progressively developing tools.
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Drewniak, Dagmara. "“Storytelling is an ancient art”: Stories, Maps, Migrants and Flâneurs in Arnold Zable’s Selected Texts." Australia, no. 28/3 (January 15, 2019): 109–23. http://dx.doi.org/10.7311/0860-5734.28.3.10.
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Nadine Fresco in her research on exiled Holocaust survivors uses the term diaspora des cendres (1981) to depict the status of Jewish migrants whose lives are forever marked by their tragic experience as well as a conviction that “the[ir] place of origins has gone up in ashes” (Hirsch 243). As a result, Jewish migrants and their children have frequently resorted to storytelling treated as a means of transferring their memories, postmemories and their condition of exile from the destroyed Eastern Europe into the New World. Since “[l]iterature of Australians of Polish-Jewish descent holds a special place in Australian culture” (Kwapisz Williams 125), the aim of this paper is to look at selected texts by one of the greatest Jewish-Australian storytellers of our time: Arnold Zable and analyse them according to the paradigm of an exiled flâneur whose life concentrates on wandering the world, sitting in a Melbourne café, invoking afterimages of the lost homeland as well as positioning one’s status on a map of contemporary Jewish migrants. The analyses of Zable’s Jewels and Ashes (1991) and Cafe Scheherazade (2001) would locate Zable as a memoirist as well as his fi ctional characters within the Australian community of migrants who are immersed in discussing their un/belonging and up/rootedness. The analysis also comprises discussions on mapping the past within the context of the new territory and the value of storytelling.
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Sabrina, Nur Annisa Putri, Elpawati E, and Achmad Tjachja Nugraha. "ANALISIS PENGARUH CITRA MEREK, KUALITAS PRODUK, HARGA DAN DIFERENSIASI PRODUK TERHADAP KEPUTUSAN PEMBELIAN PIZZA HUT DI JAKARTA BARAT." AGRIBUSINESS JOURNAL 12, no. 2 (July 15, 2019): 148–56. http://dx.doi.org/10.15408/aj.v12i2.11865.
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The development of science and technology causing the rapid economic sectors and culture also affected. The development in culture sectors also can’t be avoided, one example of the development in this sectors is a lot of fast food are sold in Indonesia. According to the new survey of Master Card (titled Consumer Purchasing Priorities) contained in Suara Pembaharuan 2016, 805 Indonesian’s people prefer to eat on the fast food outlet, in the second rank are food court with 615, and in the third rank are middle class restaurant or cafe with 225. Meanwhile, there is 1% Indonesian’s people who choose to come to restaurant for fine dining. Pizza Hut is the first fast food in Indonesia which serve pizza as a main dish. Consumer’s behaviour is the most to be considered remember all of that company did constituted by the market demands. Purpose of this research are to (1) know the characteristic of Pizza Hut consumer in West Jakarta; (2) know the influence of brand image to decision purchase of Pizza Hut in West Jakarta; (3) know the influence of product quality to decision purchase of Pizza Hut in West Jakarta; (4) know the influence of prices to decision purchase of Pizza Hut in West Jakarta; and (5) know the influence of product diferentiation to purhase decision of Pizza Hut in West Jakarta. Based on result in this research, characteristic of Pizza Hut consumer are consumer with the age range of the 20-30 years, women, with employment status is student or college student, know about pizza hut product from banner or commercial advertisement and buy pizza hut just one time in a month. Result of regression multiple linear analysis are brand image, product quality and product diferentiation are not significant influence to purhase decision of pizza hut in trust rate of 95%. Although of that, prices is significant influence to purchase decision of Pizza Hut product in West Jakarta.
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Saputra, Eko. "NGAJI ASIK SAMBIL NGOPI: STRATEGI BRANDING KOMUNITAS “TERAS DAKWAH” DI YOGYAKARTA, INDONESIA (LEARNING ISLAM WHILE ENJOYING COFFEE: A BRANDING STRATEGY OF “TERAS DAKWAH" COMMUNITY IN YOGYAKARTA, INDONESIA)." Jurnal Sosiologi Reflektif 16, no. 2 (April 28, 2022): 211. http://dx.doi.org/10.14421/jsr.v16i2.2313.
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The hybridity of Muslim youth with popular culture has had an influence on the da'wah packaging strategy carried out by many da'wah communities in Indonesia, including the Teras Da'wah Community in Yogyakarta, Indonesia. The packaging of da'wah with a more popular, fun, and modern cover has made Teras Da'wah as one of da'wah communities with the most wanted by many younger Muslims in Indonesia. This article aims to describe how the branding strategy is run by the Teras Da'wah Community using a qualitative research approach, namely through ethnographic and netnographic studies for six (6) months in the Teras Da'wah Community. The results of the study reveal that the appreciation of popular culture among young people today has a significant role in 'attracting' these millennials to recite the Koran in a relaxed but serious manner at Teras Da'wah. The form of this appreciation is packaged from the selection of an attractive logo, the interior of the room that resembles a cafe, the study content packaged in slang and the clustering of the study program according to the level of understanding of the members. The consequence of this strategy is the increasingly hybrid identity of Muslim youth in Indonesia due to the intersection between Islamic values and popular culture that has long been attached to them.Hibriditas anak-anak muda muslim dengan budaya populer telah membawa pengaruh pada strategi pengemasan dakwah yang dilakukan oleh banyak komunitas dakwah di Indonesia. Salah satu diantaranya yang telah menerapkan strategi tersebut adalah Komunitas Teras Dakwah di Yogyakarta, Indonesia. Pengemasan dakwah dengan cover yang lebih ngepop, fun, dan modern telah menjadikan Teras Dakwah sebagai komunitas dakwah yang diminati oleh banyak anak-anak muda muslim di Indonesia. Artikel ini bertujuan untuk mendeskripsikan bagaimana strategi branding yang dilakukan oleh Komunitas Teras Dakwah dengan menggunakan pendekatan penelitian kualitatif, yakni melalui studi etnografi dan netnografi selama enam (6) bulan di Komunitas Teras Dakwah. Hasil penelitian mengungkapkan bahwa apresiasi Teras Dakwah terhadap budaya populer di kalangan anak muda saat ini memiliki peran signifikan dalam ‘menarik’ milenial ini untuk mengaji dengan santai tapi serius di Teras Dakwah. Wujud apresiasi ini dikemas dari pemilihan logo yang menarik, interior ruangan yang menyerupai kafe, konten kajian yang dikemas dengan bahasa gaul dan klusterisasi program kajian sesuai dengan tingkat pemahaman anggota. Konsekuensi dari strategi ini adalah semakin hibridnya identitas anak-anak muslim di Indonesia akibat persinggungan antara nilai-nilai Islam dengan budaya populer yang sudah lama melekat pada diri mereka.
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Hromyk, Oksana. "Current Tendencies Analysis of Coffee Industry in Ukraine." Restaurant and hotel consulting. Innovations 5, no. 2 (December 27, 2022): 250–66. http://dx.doi.org/10.31866/2616-7468.5.2.2022.270105.
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Topicality.The popularity of coffee industry is high. This business is actively developing in Ukraine. A coffee shop is an establishment where the high quality of the product, as well as the development of coffee consumption culture are always in the first place. An important component is the commercial activity on which the coffee industry is built. This is an indicator of the right culture: more often Ukrainians prefer coffee in some cafe or restaurant, or even takeaway, instead of home or office coffee. However, COVID 19 pandemic affected the decrease in visits by Ukrainians to coffee shops, cafes, restaurants and other catering establishments. The main share of coffee consumption is an imported product. Ukrainian production is formed by manufacturers who import coffee beans as a raw material, and in Ukraine they roast, receive, package, produce an article without caffeine, and sell it under their own brand. The fiercest competition is typical in low segments of coffee drinks and grains.
The aim of the study and its methods. The purpose of this article is to analyse the current state of Ukrainian coffee industry. When conducting the research, general scientific methods have been applied: scientific and theoretical elaborations of blighty and foreign scientists, informational materials of statistical and reference publications, analysis, synthesis, generalisation, comparison.
Results. The article analyses the current state of coffee industry in Ukraine. The greatest producers of coffee and the most popular brands of grain and ground coffee in Ukraine have been characterised. The structure of coffee consumption in Ukraine by types and in quantitative indicators has been analysed. It has been noted that Ukrainian coffee industry is represented by a large number of small operators who open their mobile coffee shops in places with high traffic. This is due to small initial investments, a short payback period, and the possibility to change the location. Since the tendencies of the world coffee market have a significant impact on coffee industry in Ukraine, the demand is also growing steadily. The amount of coffee import in Ukraine exceeded the volume of exports. In 2020, the scope of export reached 15,0 million dollars, USA, while in 2021 it increased to 15,9 million dollars, USA. The amount of import increased from 251,3 million dollars, USA in 2020 to 266,7 million dollars, USA in 2021. The import of coffee beans is aimed at meeting domestic consumers’ needs. Ukraine is completely dependent on import, due to the non-compliance with natural and climatic conditions, because growing coffee on the territory of Ukraine is impossible.
Conclusions and discussion. So, in the course of this study, it has been established that coffee industry in Ukraine is developing more actively and even creating competition. A significant place in the restaurant industry is occupied by coffee shops, which increase competition and increase the quality of provided services. Modern coffee shops are restaurant establishments where coffee is made at a new level, with an individual approach to each guest. The current types of restaurant industry establishments are multi-format, so the creation of a national coffee shop is one of the advertising means of our country. Cafes with 3–4 seats are gaining popularity in Kyiv. On the other hand, recently, popular “to go” coffee shops are losing their market key positions. The market of coffee industry is going to grow, and become saturated after the end of the full-scale war. The infrastructure is going to be restored, and the restaurant industry has all the chances to be developed in the eastern region of Ukraine.
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ЛИ, Цзин, and Любовь Александровна ЗОЛОТАРЁВА. "Отражение стереотипных представлений о русских людях в практике преподавания русского языка как иностранного (на примере китайских учебников)." Известия Восточного института 46, no. 2 (2020): 6–14. http://dx.doi.org/10.24866/2542-1611/2020-2/6-14.
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В статье анализируются китайские учебники русского языка с точки зрения отражения в них стереотипных представлений о поведении русского человека в различных бытовых ситуациях. Материал ограничен темами первого года обучения, в течение которого у китайских студентов языковых факультетов закладываются основы знаний о русской культуре, это темы «Семья», «О себе», «Учёба», «Рабочий день», «В гостях», «Отдых», «У врача», «В кафе», «Магазины, покупки». Авторы привлекают внимание к спорным моментам в учебном материале, требующем специального комментария преподавателя русского языка. В статье анализируются как положительные, так и отрицательные стереотипы, которые служат основой для создания первичного образа представителя русской культуры. При анализе материала был использован сопоставительный метод. русский язык как иностранный, русский язык в Китае, стереотипные представления, китайские учебники русского языка, «Русский язык в университетах (Восток)», стереотипы о русских именах, внешность русских, поведение и привычки русских людей, устаревшая информация The article analyzes Chinese textbooks of the Russian language from the point of view of their reflection of stereotypical ideas about the behavior of a Russian person in various everyday situations. The material is limited to the topics of the first year of study, during which Chinese students of language faculties learn the basics of knowledge about Russian culture. The list of topics includes: “Family”, “About Me”, “Study”, “Workday”, “Away”, “Rest”, “At the doctor”, “In a cafe”, and “Supermarkets, purchases”. The authors draw attention to controversial points in the educational material, requiring special commentary from the Russian language teacher. The article analyzes both positive and negative stereotypes, which are the basis for creating the primary image of a representative of Russian culture. The image of a Russian person represented in first-year textbooks is not ideal, both advantages and vices are noted. The authors also point out that some of the material in the Chinese textbooks no longer corresponds to today’s Russian reality and needs to be corrected. A comparative method is used in the analysis of the material. Russian as a foreign language, Russian language in China, stereotypes, Chinese textbooks of the Russian language, “Russian language at universities (East)”, stereotypes about Russian names, Russian appearance, behavior and habits of Russian people, outdated information
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Zaranggi, Ahmed. "The Influence of Remembrance on Sobriety Modern Society at Café Rumi." Proceeding International Conference on Science and Engineering 2 (March 1, 2019): 283–90. http://dx.doi.org/10.14421/icse.v2.104.
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The spiritual crisis is so much engulfed by the urban community with its materialistic life, this leads to a vacuum of heart and leads to serious stress and turmoil. This turmoil mobilized everyone to overcome it, including Café Rumi worshipers. A spiritual café that offers spiritual needs that are being sought by the urban community. Through the application of routine zikir rituals, it can be a solution to this problem This study aims to find out whether the typical ritual remembrance activities at Café Rumi can have a significant impact and influence on the sobriety of the pilgrims who regularly attend zikir activities that are there. This study uses qualitative methods with descriptive analysis approach through direct observation, in-depth interviews and documentation process. After that, it is done with the data analysis process, compared, described later until the final conclusions are obtained. The target of being a researcher is the Cafe Rumi congregation, most of whom are city residents. This study yields conclusions in the form of the meaning of remembrance in Café Rumi is not merely a ritual but rather a zikir in an act of sharing with others. And from the reading side there is a modification of the addition and subtraction of the number of zikir. Zikir activities are carried out in three forms, namely spiritual meditation with zikir together led by kiyai, culture through sholawatan and Sufi dances accompanied by hadrah music, and social activities in the form of sharing food to people in need at a crossroads. The ritual of the typical Sufi ritual of remembrance is also able to increase the sobriety for worshipers at Café Rumi.
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Luens, Karin M., Marilyn A. Travis, Ben P. Chen, Beth L. Hill, Roland Scollay, and Lesley J. Murray. "Thrombopoietin, kit Ligand, and flk2/flt3 Ligand Together Induce Increased Numbers of Primitive Hematopoietic Progenitors From Human CD34+Thy-1+Lin− Cells With Preserved Ability to Engraft SCID-hu Bone." Blood 91, no. 4 (February 15, 1998): 1206–15. http://dx.doi.org/10.1182/blood.v91.4.1206.
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Abstract CD34+Thy-1+Lin− cells are enriched for primitive hematopoietic progenitor cells (PHP), as defined by the cobblestone area-forming cell (CAFC) assay, and for bone marrow (BM) repopulating hematopoietic stem cells (HSC), as defined by the in vivo SCID-hu bone assay. We evaluated the effects of different cytokine combinations on BM-derived PKH26-labeled CD34+Thy-1+Lin− cells in 6-day stroma-free cultures. Nearly all (>95%) of the CD34+Thy-1+Lin− cells divided by day 6 when cultured in thrombopoietin (TPO), c-kit ligand (KL), and flk2/flt3 ligand (FL). The resulting CD34hiPKHlo (postdivision) cell population retained a high CAFC frequency, a mean 3.2-fold increase of CAFC numbers, as well as a capacity for in vivo marrow repopulation similar to freshly isolated CD34+Thy-1+Lin− cells. Initial cell division of the majority of cells occurred between day 2 and day 4, with minimal loss of CD34 and Thy-1 expression. In contrast, cultures containing interleukin-3 (IL-3), IL-6, and leukemia inhibitory factor contained a mean of 75% of undivided cells at day 6. These CD34hi PKHhi cells retained a high frequency of CAFC, whereas the small population of CD34hiPKHlo postdivision cells contained a decreased frequency of CAFC. These data suggest that use of a combination of TPO, KL, and FL for short-term culture of CD34+Thy-1+Lin− cells increases the number of postdivision PHP, measured as CAFC, while preserving the capacity for in vivo engraftment.
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Araki, Hiroto, Nadim Mahmud, Mohammed Milhem, Hetal S. Patel, Rafael Nunez, Edward Bruno, and Ronald Hoffman. "CD34+CD90+ Cord Blood Cells Which Have Undergone Multiple Cell Divisions Retain Marrow Repopulating Potential." Blood 104, no. 11 (November 16, 2004): 3228. http://dx.doi.org/10.1182/blood.v104.11.3228.3228.
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Abstract We have previously shown that the sequential addition of a hypomethylating agent, 5-aza-2′-deoxyctidine (5azaD) and a histone deacetylase inhibitor, trichostatin A (TSA) is capable of changing the fate of adult bone marrow CD34+ cells (Milhem M et al Blood 2004). We have now studied whether these drugs could alter the behavior of dividing CD34+CD90+ cord blood (CB) cells. The 5azaD/TSA treated cultures yielded 10 times greater numbers of CD34+CD90+ cells as compared to the cultures containing cytokines alone after 9 days of culture. The 5azaD/TSA treated cultures contained 2 fold greater numbers of colony forming cells (CFC) and 14 fold greater numbers of long-term (5wk) cobblestone area forming cells (CAFC) in comparison to culture containing cytokines alone. Although the CFC/CAFC plating efficiency of cells in cultures exposed to cytokines alone declined during the time of incubation, the cloning efficiency of cells exposed to 5azaD/TSA was equivalent to that of primary CD34+ cells. In order to determine the effects of cell division on the behavior of CD34+CD90+ cells in the 5azaD/TSA treated cultures, we utilized the cytoplasmic dye, CFSE. All of the CD34+CD90+ cells within the 5azaD/TSA pre-treated cultures divided at least once after 9 days of culture. 60% of the 5azaD/TSA treated CD34+CD90+ cells divided 5 times or more while 40% divided 1–4 times. The CD34+CD90+ cells lacking 5azaD/TSA pre-treatment underwent more cell divisions (90%, 5 or more divisions). The CD34+CD90+ cells pre-treated with 5azaD/TSA which had undergone 1-2 cell divisions had 11 fold greater numbers of CFU-Mix and 9 fold greater number of CAFC as compared to CD34+CD90+ cell population cultured in presence of cytokines alone. Furthermore CD34+CD90+ cells having 5 and more divisions had 4 fold more CFU-mix and 6.5 fold more CAFC in comparison to cells lacking 5azaD/TSA exposure. The CD34+CD90+ cells experiencing 1–4 divisions had 60 fold greater number of CFU-mix and 54 fold more CAFC in contrast to culture treated with cytokines alone. The in vivo SCID repopulating potential of CD34+CD90+ cells generated in presence or absence of 5azaD/TSA was then evaluated. When 5x104 CD34+CD90+ cells having undergone 1-2 cell divisions were re-isolated from 5azaD/TSA pre-treated cultures, all mice contained human hematopoietic cells. In addition, 1 of 3 mice transplanted with CD34+CD90+ cells (5x104) having undergone 3 and more cell divisions isolated from cultures pre-treated with 5azaD/TSA also displayed human hematopoietic engraftment. Furthermore 1 of 3 mice transplanted with equal numbers of the 5azaD/TSA pre-treated CD34+CD90+ cells having undergone 5 and more cell divisions also had evidence of human multilineage hematopoietic engraftment. By contrast, an equivalent number of CD34+CD90+ cells which had undergone more than 3 or more than 5 cell divisions from the cultures containing cytokines alone were incapable of engrafting NOD/SCID mice. These data suggest that the sequential addition of 5azaD and TSA ex vivo is not only capable of expanding the numbers of CD34+CD90+ cells and assayable progenitor cells but also capable of preserving their SCID repopulating potential. We conclude that 5azaD/TSA treatment of CD34+CD90+ cells results in their retention of the cellular program required to maintain their marrow repopulating potential despite their undergoing multiple cell divisions.
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Khatko, Z. N., G. V. Karamushko, T. B. Kolotiy, and L. V. Lyubimova. "Development of a university healthy diet system: high student cuisine." New Technologies 16, no. 5 (December 31, 2020): 45–54. http://dx.doi.org/10.47370/2072-0920-2020-16-5-45-54.
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The purpose of the research is to develop a university system of healthy nutrition, which determines the preservation and strengthening of students’ health by optimizing their nutrition in educational institutions, using an innovative menu and «high cuisine» techniques to organize a high-class student cafe. The used terminology, graphic materials and tools comply with GOST R ISO 21500-2014; UNIDO business planning methodology; methodology for developing EBRD business strategy. The peculiarities of eating behavior of students of Maykop State Technological University and the Adygh State University of the Republic of Adygea have been investigated. Peculiarities of students’ perception of the quality of services of public catering enterprises, an assessment of the structure of expenses for the acquisition of various dishes at catering enterprises, an assessment of the impact of the social status of students on the structure of consumption of products of public catering enterprises, etc. have been considered. Particular attention is paid to identifying nutritional problems existing among students in the region. On the basis of the research the directions for improving healthy nutrition of students have been identified. A university system of healthy nutrition for students has been developed, based on the philosophy of youth taste and preferences: food behavior and reasonable quality: balance in basic substances, with specified beneficial properties, a recommended diet consisting only of healthy food products. The developed system of healthy nutrition will ensure the formation and development of food culture: aesthetic taste for food and its consumption in order to reorient young people from fast and unhealthy nutrition to a healthy diet, using «high cuisine» techniques (careful preparation and careful presentation of dishes / products designed to form a sustainable high motivation for healthy eating, which persists throughout the rest of life, the quality of which can be predicted with predominant indicators. Increasing the efficiency of a public catering enterprise for students will ensure the use of the procurement automation system; takeaway cooking and delivery service; QR-code is a progressive way to get accurate on-line statistics; electronic menu.
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De Lima, Maria Alzete, Francisca Tereza de Galiza, Fernanda Rafaela Martins Xavier, Jemima Rafaela Rodrigues de Medeiros, Fabiana Emanuela Câmara de Moura, and Larissa Lucena de Araújo. "Learning culture in nephrology / Cultura de aprendizagem em nefrologia / La cultura del aprendizaje en nefrología." Revista de Enfermagem da UFPI 5, no. 1 (March 1, 2016): 73. http://dx.doi.org/10.26694/reufpi.v5i1.4942.
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Objetivo: identificar conhecimento da equipe de enfermagem sobre cuidado com paciente em hemodiálise. Metodologia: trata-se de estudo qualitativo do tipo pesquisa ação, realizado com toda a equipe, 12 profissionais de enfermagem em clínica de hemodiálise, perguntas norteadoras, observação e registro foram usados na coleta, em junho de 2014, aprovação comitê de ética em pesquisa CAAE: 0422.0.045.000-11. Resultados: identificou-se a necessidade de ampliação do conhecimento sobre os cuidados com paciente em hemodiálise. Existe imprecisão na formulação de conceitos, características do tratamento e necessidade de ampliar conhecimento sobre a doença renal. Além da reduzida quantidade de profissionais que facilitem a aprendizagem e realizem orientações sobre o autocuidado no processo de adaptação com o tratamento hemodialítico. Conclusões: portanto, defende-se que os profissionais devam exercer a consciência crítica para o processo contínuo de formação para promover o cuidado apoiado.
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Drize, Nina, Daria Svinareva, Irina Nifontova, and Joseph Chertkov. "Parathyroid Hormone (1–34) Treatment Induces Hematopoietic Precursor Cell Expansion in Murine Long-Term Bone Marrow Culture." Blood 104, no. 11 (November 16, 2004): 4164. http://dx.doi.org/10.1182/blood.v104.11.4164.4164.
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Abstract Parathyroid hormone (PTH) induces activation and increases the number of osteoblasts. An increase the stem cells number (Lin-Sca1+cKit+) was observed in mice after 4 weeks of PTH treatment, that suggest osteoblasts participation in regulation of hematopoiesis (Calvi, et al., 2003, Nature, 425, 841). Long-term bone marrow culture (LTBMC) was used for the study of PTH influence on hematopoietic progenitors of different stages of maturation. Rat PTH (1–34) (final concentration, 10−7M) was supported during whole culture period. Cell number, colony-forming units in culture (CFU-GM, CFU-C-21 days) and cobblestone area forming cells (CAFC) were measured during 10 weeks. The number of mature cells in culture did not changed during PTH treatment. The number of studied progenitors did not changed significantly during 3 and 6 weeks of PTH treatment. The number of CFU-GM, CFU-C-21 days and CAFC 14 days, CAFC 21 days, CAFC 28 days increased 7 –10 fold in suspension fraction of LTBMC after 10 weeks of PTH treatment. Table 1. Number of different precursor cells in suspension fraction of LTBMC after PTH treatment. To evaluate the possibility of stem cells expansion by using of PTH treated adherent cell layers (ACL) of LTBMC, the PTH-treated for 4 and 8 weeks cultures, were irradiated with 40 Gy and seeded with 2 × 106 bone marrow cells depleted of adherent cells. Following 24 hours the number of survived CAFC 8 – 28 was analyzed. On PTH-treated ACL the number of CAFC 21 increased 2,7 ± 0,4 fold as compared with fresh bone marrow or culture with non-treated ACLs. The number of CAFC 28, which characterizes the number of marrow repopulating cells, increased 2 fold only after cultivation on ACL treated with PTH for 8 weeks. Table 2. CAFC number in bone marrow cultivated 24 hours on PTH treated ACL of LTBMC The data suggest possibility to increase stem cell expansion ex vivo on pharmacologically manipulated microenvironment. Time of cultivation, weeks Specificity of progenitor Number of precursors, per 100000 cells Control Control PTH treatment 3 CFU-GM 50 ± 5.2 77 ± 5.6 6 CFU-GM 33 ± 4 31 ± 5.9 10 CFU-GM 20 ± 4.8 147.5 ± 3.5 3 CFU-C-21 55 ± 5.9 76.6 ± 11.2 10 CFU-C-21 11.5 ± 1.7 102.0 ± 2.0 3 CAFC-7 21.6 20.3 6 CAFC-7 25.9 26.9 10 CAFC-7 0.9 3.0 3 CAFC-21 1.01 0.69 6 CAFC-21 0.43 0.48 10 CAFC-21 0.28 3.11 3 CAFC-28 0.32 0.55 6 CAFC-28 0.12 0.14 10 CAFC-28 0.1 0.82 Age of CAFC (days) Number of CAFC per 2 x 10^6 bone marrow cells not treated PTH (4 weeks) PTH (8 weeks) CAFC-7 424.1 804.5 647.5 CAFC-14 262.5 529.8 295.2 CAFC-21 93.5 272.7 226.2 CAFC-28 38.3 30.1 79.2
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Cofer, Judith Ortiz. "Corazon's Cafe." Callaloo 17, no. 3 (1994): 715. http://dx.doi.org/10.2307/2931847.
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Papale, Maria, Ida Romano, Ilaria Finore, Angelina Lo Giudice, Alessandro Piccolo, Silvana Cangemi, Vincenzo Di Meo, Barbara Nicolaus, and Annarita Poli. "Prokaryotic Diversity of the Composting Thermophilic Phase: The Case of Ground Coffee Compost." Microorganisms 9, no. 2 (January 21, 2021): 218. http://dx.doi.org/10.3390/microorganisms9020218.
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Waste biomass coming from a local coffee company, which supplied burnt ground coffee after an incorrect roasting process, was employed as a starting material in the composting plant of the Experimental Station of the University of Naples Federico II at Castel Volturno (CE). The direct molecular characterization of compost using 13C-NMR spectra, which was acquired through cross-polarization magic-angle spinning, showed a hydrophobicity index of 2.7% and an alkyl/hydroxyalkyl index of 0.7%. Compost samples that were collected during the early “active thermophilic phase” (when the composting temperature was 63 °C) were analyzed for the prokaryotic community composition and activities. Two complementary approaches, i.e., genomic and predictive metabolic analysis of the 16S rRNA V3–V4 amplicon and culture-dependent analysis, were combined to identify the main microbial factors that characterized the composting process. The whole microbial community was dominated by Firmicutes. The predictive analysis of the metabolic functionality of the community highlighted the potential degradation of peptidoglycan and the ability of metal chelation, with both functions being extremely useful for the revitalization and fertilization of agricultural soils. Finally, three biotechnologically relevant Firmicutes members, i.e., Geobacillus thermodenitrificans subsp. calidus, Aeribacillus pallidus, and Ureibacillus terrenus (strains CAF1, CAF2, and CAF5, respectively) were isolated from the “active thermophilic phase” of the coffee composting. All strains were thermophiles growing at the optimal temperature of 60 °C. Our findings contribute to the current knowledge on thermophilic composting microbiology and valorize burnt ground coffee as waste material with biotechnological potentialities.
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Breems, Dimitri A., Ellen A. W. Blokland, Karen E. Siebel, Angelique E. M. Mayen, Lilian J. A. Engels, and Rob E. Ploemacher. "Stroma-Contact Prevents Loss of Hematopoietic Stem Cell Quality During Ex Vivo Expansion of CD34+ Mobilized Peripheral Blood Stem Cells." Blood 91, no. 1 (January 1, 1998): 111–17. http://dx.doi.org/10.1182/blood.v91.1.111.
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Abstract Stroma-supported long-term cultures (LTC) allow estimation of stem cell quality by simultaneous enumeration of hematopoietic stem cell (HSC) frequencies in a graft using the cobblestone area forming cell (CAFC) assay, and the ability of the graft to generate progenitors in flask LTC (LTC-CFC). We have recently observed that the number and quality of mobilized peripheral blood stem cells (PBSC) was low in patients having received multiple rounds of chemotherapy. Moreover, grafts with low numbers of HSC and poor HSC quality had a high probability to cause graft failure upon their autologous infusion. Because ex vivo culture of stem cells has been suggested to present an attractive tool to improve hematological recovery or reduce graft size, we have studied the possibility that such propagation may affect stem cell quality. In order to do so, we have assessed the recovery of different stem cell subsets in CD34+ PBSC after a 7-day serum-free liquid culture using CAFC and LTC-CFC assays. A numerical expansion of stem cell subsets was observed in the presence of interleukin-3 (IL-3), stem cell factor, and IL-6, while stroma-contact, stromal soluble factors, or combined addition of FLT3-ligand and thrombopoietin improved this parameter. In contrast, ex vivo culture severely reduced the ability of the graft to produce progenitors in LTC while stromal soluble factors partly abrogated this quality loss. The best conservation of graft quality was observed when the PBSC were cultured in stroma-contact. These data suggest that ex vivo propagation of PBSC may allow numerical expansion of various stem cell subsets, however, at the expense of their quality. In addition, cytokine-driven PBSC cultures require stroma for optimal maintenance of graft quality.
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Breems, Dimitri A., Ellen A. W. Blokland, Karen E. Siebel, Angelique E. M. Mayen, Lilian J. A. Engels, and Rob E. Ploemacher. "Stroma-Contact Prevents Loss of Hematopoietic Stem Cell Quality During Ex Vivo Expansion of CD34+ Mobilized Peripheral Blood Stem Cells." Blood 91, no. 1 (January 1, 1998): 111–17. http://dx.doi.org/10.1182/blood.v91.1.111.111_111_117.
Full textAbstract:
Stroma-supported long-term cultures (LTC) allow estimation of stem cell quality by simultaneous enumeration of hematopoietic stem cell (HSC) frequencies in a graft using the cobblestone area forming cell (CAFC) assay, and the ability of the graft to generate progenitors in flask LTC (LTC-CFC). We have recently observed that the number and quality of mobilized peripheral blood stem cells (PBSC) was low in patients having received multiple rounds of chemotherapy. Moreover, grafts with low numbers of HSC and poor HSC quality had a high probability to cause graft failure upon their autologous infusion. Because ex vivo culture of stem cells has been suggested to present an attractive tool to improve hematological recovery or reduce graft size, we have studied the possibility that such propagation may affect stem cell quality. In order to do so, we have assessed the recovery of different stem cell subsets in CD34+ PBSC after a 7-day serum-free liquid culture using CAFC and LTC-CFC assays. A numerical expansion of stem cell subsets was observed in the presence of interleukin-3 (IL-3), stem cell factor, and IL-6, while stroma-contact, stromal soluble factors, or combined addition of FLT3-ligand and thrombopoietin improved this parameter. In contrast, ex vivo culture severely reduced the ability of the graft to produce progenitors in LTC while stromal soluble factors partly abrogated this quality loss. The best conservation of graft quality was observed when the PBSC were cultured in stroma-contact. These data suggest that ex vivo propagation of PBSC may allow numerical expansion of various stem cell subsets, however, at the expense of their quality. In addition, cytokine-driven PBSC cultures require stroma for optimal maintenance of graft quality.
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Funahashi, H. "244 EFFECT OF β-MERCAPTOETHANOL IN A TRANSIENT-IVF SYSTEM ON SPERM PENETRATION INTO PORCINE IVM OOCYTES." Reproduction, Fertility and Development 17, no. 2 (2005): 272. http://dx.doi.org/10.1071/rdv17n2ab244.
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This study was carried out to determine the effect of β-mercaptoethanol (bME; at 50 μM) during a brief co-culture and the additional culture of inseminated oocytes on sperm penetration of porcine IVM oocytes. Cumulus-oocyte complexes (COCs) collected from 3–∼6-mm follicles were cultured in modified NCSU-37 supplemented with bME, eCG, hCG, and dibutyryl cAMP for 20 h and then in the same medium without eCG, hCG, and dibutyryl cAMP for additional 24 h. After IVM, the oocytes were denuded with 0.1% hyarulonidase, randomly divided into three groups (as described below) and fertilized in vitro. Treatment 1 (T1, control): Oocytes were co-cultured with fresh spermatozoa for 10 min in modified M199 medium (mM199) supplemented with 5 mM caffeine (+caff) in the absence of bME (bME-), then washed gently with caffeine-free mM199, and cultured for further 9 h in mM199; T2: co-culture for 10 min in mM199+caff bME− and additional culture in mM199 in the presence of bME (bME+); and T3: co-culture for 10 min in mM199+caff bME+ and additonal culture in mM199 bME+. At 9 h after insemination, oocytes were fixed and stained for the evaluation of sperm penetration. Results from four replicates were analyzed by ANOVA and Duncan's multiple range test. Sperm penetration rate was lower in T3 (79.8% in 92 oocytes) compared with T1 (95.3% in 84 oocytes) and T2 (93.3% in 93 oocytes). Percentage of eggs with male and female pronuclei in penetrated oocytes was higher in T1 (89.6%) than in T2 (56.4%) and T3 (57.2%). Monospermy rate was lower in T1 (48.9%) than in T2 (59.5%) and T3 (64.3%). These results demonstrated that, although the presence of β-mercaptoethanol during co-culture and the additional culture reduced the fertilization rate, the presence during the additional culture period affected the process of fertilization and increased the incidence of monospermy at 9 h after insemination. In conclusion, the progression of sperm penetration may be controlled by utilization of β-mercaptoethanol in porcine IVF system. This work was supported by JSPS-KAKEN (C16580230).
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Gilfillan, Siv, Simone Kraner, Katharina S. Goetze, Matthias Schiemann, Stefanie Marz, Christian Peschel, and Robert A. J. Oostendorp. "The Combination of Stem Cell Factor and Oncostatin M Maintains Cord Blood-Derived NOD/SCID-Repopulating Cells." Blood 106, no. 11 (November 16, 2005): 4267. http://dx.doi.org/10.1182/blood.v106.11.4267.4267.
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Abstract We here investigated whether receptor tyrosine kinase signaling (ckit, flt3) was sufficient to maintain hematopoietic stem cells (HSC) in culture, or whether, like in embryonic stem cells, signaling through gp130 is also required. Sorted CD34+ AC133+ (CD33/ CD38/ CD71)- cells from human umbilical cord blood (CB) were cultured in the presence of combinations of ckit-ligand stem cell factor (SCF), flt3-ligand (FL) and different stimulators of gp130 (IL-6, IL-11, LIF, OSM, CT1 and CNTF). Of these cytokines, SCF stimulated cell division by itself. Gp130-stimulating cytokines did not give any additional cell division activity. When SCF was included in the cytokine cocktail, progenitor levels (CFC, CAFCw2, CAFCw6) was very similar for all combinations of cytokines tested after this initital 6-day culture. To study more immature progenitors, cells were harvested after 6 days of serum-free culture and cultured for another 6 weeks on FBMD-1 stromal cells (LTC-CAFC). These experiments showed that in initial 6 day cultures with FL or gp130-stimulators alone, very little hematopoietic activity remained. SCF maintained CFC, and LTC-CAFCw2 and LTC-CAFCw6 at about half the original level. However, combinations of SCF with OSM consistently increased the number of LTC-CAFCw2 and w6 about two-fold. This finding suggests that very immature hematopoietic progenitors were at least maintained in serum-free cultures. To study the most immatore cells, we performed in transplantations into sublethally irradiated NOD/SCID mice. When CB cells were cultured with SCF alone, NOD/SCID-repopulating cell (CRU) frequency (1 in 900) dropped to about 20% in 6 days (1 in 5500). Similarly, cultures without growth factors or with FL or OSM showed a decrease to less than 10% of the original CRU number. In contrast, the combination of SCF and OSM maintained CRU at about half the original level (1 in 1900). This study shows that signals through ckit and gp130 synergize to maintain CRU level. However, to achieve expansion of human NOD/SCID-repopulating cell numbers, additional signaling pathways need to be stimulated.
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Teoh, N., M. J. A. Parr, and S. R. Finfer. "Bacteraemia following Percutaneous Dilational Tracheostomy." Anaesthesia and Intensive Care 25, no. 4 (August 1997): 354–57. http://dx.doi.org/10.1177/0310057x9702500405.
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This study reports the incidence of bacteraemia following 106 consecutive bedside percutaneous tracheostomies. Post-tracheostomy blood culture results were compared with other blood cultures from the same population. The incidence of positive post-tracheostomy blood cultures was 10.4% (11/106), compared with 6.6% (7/106) for other blood cultures (odds ratio 1.64, 95% confidence interval 0.61-4.40, P=0.46). Staphylococcus epidermidis was the most common organism cultured, 7/106 (6.6%) of post-tracheostomy cultures, compared with 3/106 (2.8%) for other cultures (odds ratio 2.43, 95% confidence interval 0.61-9.65, P=0.33). The other four post-tracheostomy cultures grew an organism cultured from that patient's tracheal secretions. Seventy-four patients were receiving antibiotics at the time of tracheostomy, of these 7 (9.5%) had positive blood cultures, a similar incidence (4 of 32, 12.5%) to those not receiving antibiotics (odds ratio 0.73, 95% confidence interval 0.20-2.70, P=0.90). We conclude bacteraemia is a common complication of percutaneous tracheostomy; the causative organisms come from the patients’ trachea or skin.
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Weisel, Katja C., Kaida Wu, Lothar Kanz, and Malcolm A. S. Moore. "Long-Term Coculture of Adult Human Healthy Donor CD34+ Cells Shows Elevation of Telomeres in a Subgroup of Donors Independent of Age, Stem Cell Function and Telomerase Activity." Blood 104, no. 11 (November 16, 2004): 1695. http://dx.doi.org/10.1182/blood.v104.11.1695.1695.
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Abstract Long-term cytokine-supplemented or stromal cocultures of human CD34+ cells, particularly from cord blood (CB), show expansion of hematopoietic progenitors and stem cells. Ultimately, however cultures decline and terminally differentiate. Despite upregulation of telomerase activity in proliferating primitive hematopoietic cells, telomere shortening has generally been reported in long-term cultures of CB, bone marrow or G-CSF mobilized peripheral blood CD34+ cells. In earlier reports, we described a long-term culture of hematopoietic stem cells on a murine OP9 bone marrow stroma cell line transfected with an adenovector expressing thrombopoietin, which allowed an extensive proliferation and self-renewal of CB CD34+ cells for 4–5 months with sustained elevation of telomerase activity and without concomitant significant telomere shortening (Blood, 2004). Here, we evaluated adult healthy donor peripheral blood (PB) CD34+ cells in the same OP9/Tpo coculture system. To determine progenitor and stem cell production, standard CFC and 2ndry cobblestone area-forming cell assays (CAFC assayed at 5 weeks on MS5 stroma) were undertaken weekly with suspension cells. In addition telomere length was measured by telomere restriction fragment (TRF) assay, and telomerase activity by TRAP assay on input CD34+ cells, and weekly on culture suspension cells. Maximum total cell, CFC and CAFC production was seen in the first 4 weeks with up to 80-fold expansion in cell count, up to 4-fold expansion in CFC and up to 13-fold expansion in CAFC. Thereafter a continuous decrease in production of cells, CFC 2ndry CAFC was observed and cultures terminated at week 8. Mean telomere length of input PB CD34+ cells was 9,5 ± 0,5 kbp. After 4 weeks in culture, telomere length remained stable (9,6 ± 0,5 kbp). In 3/6 cultures terminated, cultures showed only a slightly decrease of telomere length compared to the input population (0,55 ± 0,1 kbp loss). However, in 2/6 cultures we could demonstrate an elevation of telomeres (+ 0,3 kbp) independent of a rapid loss of telomerase activity in all cultures during the culture period. Furthermore, the elevation of telomeres did not correlate with an enhanced stem/progenitor cell activity. These data confirm earlier results of granulocyte telomere change in myeloma patients following chemotherapy and tandem transplantation, where 154/193 patients showed an expected loss of telomeres during the treatment period, whereas 39/193 patients had an unexpected elevation of telomeres. We could now show in healthy donors that this phenomenon is independent of bone marrow stress due to chemotherapeutic treatment. In conclusion, we could show that the stromal coculture system with OP9/Tpo is highly effective in stem/progenitor cell expansion not only in CB but also in PB CD34+ cells. It is the first culture system, which allows expansion of hematpoietic cells without significant telomere erosion. We furthermore describe for the first time an age-independent healthy donor population which shows telomerase-independent, significant telomere elevation. Further studies have to demonstrate, if this phenomenon is potentially linked to a higher susceptibility for cancer disease.
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Glave, Thomas. "At the Cafe." Callaloo 23, no. 4 (2000): 1259–72. http://dx.doi.org/10.1353/cal.2000.0198.
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