Academic literature on the topic 'Calcein indicator'

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Journal articles on the topic "Calcein indicator"

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HUITINK, G. "Constant Calcein Blue: An indicator for spectrofluorometric calcium determination." Talanta 34, no. 4 (1987): 423–26. http://dx.doi.org/10.1016/0039-9140(87)80064-4.

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Shyichuk, Alexander, Dorota Ziółkowska, Jan Lamkiewicz, and Maria Kowalska. "Calcium Determination by Complexometric Titration with Calcein Indicator Using Webcam for Endpoint Detection." Water 17, no. 12 (2025): 1757. https://doi.org/10.3390/w17121757.

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Precise and convenient analytical methods are needed for the quantitative determination of calcium in water and food. Complexometric titration remains a reliable technique to determine calcium in milligram amounts. The titrations have been performed automatically by detecting color transitions with a webcam. Classical complexometric indicator calcein provided a sharp color transition. In diffuse reflection mode, the color appearance parameter (Hue) provides better precision and is more resistant to ambient light fluctuations compared to RGB primaries. In fluorescence mode with LED illumination
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DEMOTT, B. J. "Determination of Calcium in Cottage Cheese by Titration." Journal of Food Protection 51, no. 10 (1988): 802–3. http://dx.doi.org/10.4315/0362-028x-51.10.802.

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The calcium concentration of cottage cheese has been determined by the use of disodium ethylene diaminetetraacetate as a calcium binder and calcein as an indicator. This method of analysis compares favorably with the atomic absorption procedure.
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Nieminen, A. L., A. K. Saylor, S. A. Tesfai, B. Herman, and J. J. Lemasters. "Contribution of the mitochondrial permeability transition to lethal injury after exposure of hepatocytes to t-butylhydroperoxide." Biochemical Journal 307, no. 1 (1995): 99–106. http://dx.doi.org/10.1042/bj3070099.

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We have developed a novel method for monitoring the mitochondrial permeability transition in single intact hepatocytes during injury with t-butylhydroperoxide (t-BuOOH). Cultured hepatocytes were loaded with the fluorescence probes, calcein and tetramethylrhodamine methyl ester (TMRM). Depending on loading conditions, calcein labelled the cytosolic space exclusively and did not enter mitochondria or it stained both cytosol and mitochondria. TMRM labelled mitochondria as an indicator of mitochondrial polarization. Fluorescence of two probes was imaged simultaneously using laser-scanning confoca
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Dissard, Delphine, Gernot Nehrke, Gert Jan Reichart, Julius Nouet, and Jelle Bijma. "Effect of the fluorescent indicator calcein on Mg and Sr incorporation into foraminiferal calcite." Geochemistry, Geophysics, Geosystems 10, no. 11 (2009): n/a. http://dx.doi.org/10.1029/2009gc002417.

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Slayman, C. L., V. V. Moussatos, and W. W. Webb. "Endosomal accumulation of pH indicator dyes delivered as acetoxymethyl esters." Journal of Experimental Biology 196, no. 1 (1994): 419–38. http://dx.doi.org/10.1242/jeb.196.1.419.

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Intracellular distributions of the putative cytosolic pH indicator dyes BCECF [2',7'-bis-(2-carboxyethyl)-5(and 6)-carboxyfluorescein], C.SNARF [5(and 6)-carboxy-seminaphthorhodafluor-1], and C.SNARF-calcein have been examined in Neurospora crassa and in murine fibroblasts (NIH-3T3 cells) under conditions in which both kinds of cells produce visible microscopic vacuoles. All three dyes were administered in electroneutral forms, with the hydroxyl and carboxyl groups esterified (designated as -AM esters). As judged qualitatively from fluorescence levels, hydrolytic derivatives of the two heavily
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BUSTOS, PATRICIA L., ALINA E. PERRONE, NATALIA MILDUBERGER, MIRIAM POSTAN, and JACQUELINE BUA. "Oxidative stress damage in the protozoan parasite Trypanosoma cruzi is inhibited by Cyclosporin A." Parasitology 142, no. 8 (2015): 1024–32. http://dx.doi.org/10.1017/s0031182015000232.

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SUMMARYCyclosporin A (CsA) specifically inhibits the mitochondrial permeability transition pore (mPTP). Opening of the mPTP, which is triggered by high levels of matrix [Ca2+] and/or oxidative stress, leads to mitochondrial dysfunction and thus to cell death by either apoptosis or necrosis. In the present study, we analysed the response of Trypanosoma cruzi epimastigote parasites to oxidative stress with 5 mm H2O2, by studying several features related to programmed cell death and the effects of pre-incubation with 1 μm of CsA. We evaluated TcPARP cleavage, DNA integrity, cytochrome c transloca
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Mandavilli, Bhaskar S., Daniel Beacham, Yi-Zhen Hu, Jongtae Yang, and Aimei Chen. "New generation of fluorescent probes for cell-based measurements of caspase Activation and mitochondrial superoxide." Journal of Immunology 210, no. 1_Supplement (2023): 153.17. http://dx.doi.org/10.4049/jimmunol.210.supp.153.17.

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Abstract Cell health and stress readouts are critical indicators of altered or impaired function in normal and diseased states of cells, and work has been underway to develop improved small molecule sensor dyes compatible with traditional imaging and High Content Analysis (HCA) interrogation of apoptotic and mitochondrial stress pathways. The CellEvent™ Caspase Green dye effectively reports caspase activation, but suffers complications in assay configuration when attempting to multiplex with the Green Fluorescent Protein (GFP), calcein, or other 488 laser line tools in fluorescence microscopy.
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Mandavilli, Bhaskar S., Daniel Beacham, Yi Zhen Hu, Jongtae Yang, and Aimei Chen. "Abstract 4781: New generation of fluorescent probes for cell-based measurements of caspase activation and mitochondrial superoxide." Cancer Research 83, no. 7_Supplement (2023): 4781. http://dx.doi.org/10.1158/1538-7445.am2023-4781.

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Abstract Cell health and stress readouts are critical indicators of altered or impaired function in normal and diseased states of cells, and work has been underway to develop improved small molecule sensor dyes compatible with traditional imaging and High Content Analysis (HCA) interrogation of apoptotic and mitochondrial stress pathways. The CellEvent™ Caspase Green dye effectively reports caspase activation, but suffers complications in assay configuration when attempting to multiplex with the Green Fluorescent Protein (GFP), calcein, or other 488 laser line tools in fluorescence microscopy.
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Grabicoski, Edilaine Mauricia Gelinski, David de Souza Jaccoud-Filho, David Lee, Luciane Henneberg, and Marcos Pileggi. "Real-Time Quantitative and Ion-Metal Indicator LAMP-Based Assays for Rapid Detection of Sclerotinia sclerotiorum." Plant Disease 104, no. 5 (2020): 1514–26. http://dx.doi.org/10.1094/pdis-07-19-1455-re.

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Sclerotinia sclerotiorum is one of the most devastating and cosmopolitan plant pathogens. Rapid detection of S. sclerotiorum can provide growers an advantage in knowing what control measures should be taken to minimize crop damage and financial losses caused by it. Loop-mediated isothermal amplification (LAMP) is a fast, sensitive, and specific nucleic acid amplification method that does not require a thermal cycler. This study aimed to develop a LAMP-based assay for the specific detection of S. sclerotiorum (Ss-LAMP). A real-time quantitative LAMP reaction (Ss-qLAMP) and a calcein ion indicat
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Book chapters on the topic "Calcein indicator"

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Hanneman, Debra L., and Charles J. Wideman. "Calcic pedocomplexes—Regional sequence boundary indicators in Tertiary deposits of the Great Plains and western United States." In Paleoenvironmental Record and Applications of Calcretes and Palustrine Carbonates. Geological Society of America, 2006. http://dx.doi.org/10.1130/2006.2416(01).

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Lexa, J., J. Štohl, and V. Konečný. "The Banská Štiavnica ore district." In Epithermal Mineralization of the Western Carpathians. Society of Economic Geologists, 1999. http://dx.doi.org/10.5382/gb.31.09.

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Abstract The Banská Štiavnica ore district is in the central zone of the largest stratovolcano in the Central Slovakia Neogene Volcanic Field, which is situated at the inner side of the Carpathian arc over the Hercynian basement with the Late Paleozoic and Mesozoic sedimentary cover. Volcanic rocks of the High-K orogenic suite are of the Badenian through Pannonian age (16.5– 8.5 Ma). Their petrogenesis is closely related to subduction of flysch belt oceanic basement underneath the advancing Carpathian arc and to back-arc extension processes. The stratovolcano includes a large caldera 20 km in diameter and a late-stage resurgent horst in its centre, exposing a basement and extensive subvolcanic intrusive complex. The following stages have been recognized in the evolution of the stratovolcano: (1) formation of a large pyroxene/hornblende-pyroxene andesite stratovolcano; (2) denudation, emplacement of a diorite intrusion; (3) emplacement of a large granodiorite bell-jar pluton within the basement; (4) emplacement of granodiorite/quartz-diorite porphyry stocks and dyke clusters around the pluton; (5) caldera subsidence and its filling by biotite-hornblende andesite volcanics, emplacement of quartz-diorite porphyry sills and dykes at the subvolcanic level; (6) renewed activity of andesites from dispersed centres on slopes of the volcano; (7) uplift of a resurgent horst accompanied by rhyolite volcanics and granite porphyry dykes. The following types of ore deposits (mineralizations) have been identified in the Banská Štiavnica ore district: 1. Quartz-pyrophyllite-pyrite high-sulphidation system at Šobov, related to the diorite intrusion. 2. Magnetite skarn deposits and occurrences at contacts of the granodiorite pluton with Mesozoic carbonate rocks. Magnetite ores occur as lenses in the calcic skarns. 3. Stockwork/disseminated base metal deposit along an irregular network of fractures in apical parts of the granodiorite pluton and in remnants of basement rocks. Mineral paragenesis is simple, with leading sphalerite and galena and minor chalcopyrite and pyrite. In overlying andesites the mineralization is accompanied by metasomatic quartzites and argillites with pyrophyllite, kaolinite, illite and pyrite. 4. Porphyry /skarn copper deposits and occurrences related to granodiorite/quartz-diorite porphyry dyke clusters and stocks around the granodiorite intrusion. The mineralized zone is represented by accumulations of chalcopyrite in exo- and endo-skarns, usually of the magnesian type affected by serpentinization. Besides chalcopyrite, pyrhotite, minor bornite, chalcosite, tennantite and magnetite, rare molybdenite and gold are present. The alteration pattern around productive intrusions includes an external zone of propylitization, a zone of argillitic alteration (kaolinite — illite — pyrite) and an internal zone of phyllic alteration (quartz — sericite — pyrite). Biotitization is rare and limited to porphyry intrusions. 5. Intrusion related “mesothermal” gold deposit in an andesitic environment just above the granodiorite intrusion. Gold of high fineness with base metal mineralization is contained in brecciated and/or banded quartz veins of subhorizontal orientation, parallel to the surface of granodiorite pluton. At least the first phase of mineralization is older than quartz-diorite porphyry sills, which separate granodiorite and blocks of mineralized andesite. 6. Hot spring type advanced argillic systems in the caldera filling. Silicites and opalites accompanied by kaolinite, alunite and pyrite grade downward into smectite dominated argillites. 7. Vein type epithermal precious/base metal deposits and occurrences as a result of the long lasting interaction among structural evolution of the resurgent horst and evolving hydrothermal system, extensive intrusive complex and deep seated siliceous magma chamber serving as heat and magmatic fluid source. Three types of epithermal veins occur in a zonal arrangement: (a) base metal veins ± Au with transition to Cu ± Bi mineralization at depth in the east/central part of the horst, (b) Ag — Au veins with minor base metal mineralization and (c) Au — Ag veins located at marginal faults of the horst. Isotopic composition of oxygen and hydrogen in hydrothermal fluids indicate mixing of magmatic and meteoric component (with generally increasing proportion of meteoric component towards younger mineralization periods?). Veins are accompanied by zones of silicification, adularization and sericitization, indicating a low sulphidation environment. 8. Replacement base metal mineralization of a limited extent in the Mesozoic carbonate rocks next to sulphide rich epithermal base metal veins.
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Conference papers on the topic "Calcein indicator"

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MOROZ, Hryhorii. "AGROPEDOGENIC TRANSFORMATION OF PHYSICAL PROPERTIES OF SOILS OF MEDIUM-DRY STEPPE PEDO-ECOTONE IN THE NORTHWEST OF THE BLACK SEA REGION." In RURAL DEVELOPMENT. Aleksandras Stulginskis University, 2018. http://dx.doi.org/10.15544/rd.2017.184.

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The anthropogenic transformation of soil physical properties of the transition stripe from the dry to the middle steppe in the Northwest of the Black Sea region is considered and analyzed on the basis of comparison of indicators characterizing the properties of the cultivable and subcultivable horizons. The signs of negative influence of agricultural use on the most important indices of physical properties of soils are investigated. Significant degradation of the physical properties of the arable horizons (in comparison with tillable and subcultivable horizons), as well as deterioration of the
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Brampton, Christopher J., Hyunsun A. Kim, and James L. Cunningham. "Applications of 3D Level Set Topology Optimization." In ASME 2012 International Design Engineering Technical Conferences and Computers and Information in Engineering Conference. American Society of Mechanical Engineers, 2012. http://dx.doi.org/10.1115/detc2012-70870.

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Level set topology optimization defines the solution using the level set function values stored at the nodes of a regular finite element grid. These values represent a signed distance function which indicates the distance from each node to the structural boundaries. During optimization, nodal sensitivities are used to update the level set function values, moving the boundaries to create a more optimal structure. This paper presents two applications of the 3D level set topology optimization procedure aiming to minimize structural compliance subject to a volume constraint. The first application
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Wang, Leyan, Jian Zhang, Xuejian Yao, and Hao Ping. "Remaining Useful Life Prediction Method for Highway Electromechanical Equipment Based on Bayesian Algorithm-Optimized CNN-LSTM Model." In 2024 International Conference on Smart Transportation Interdisciplinary Studies. SAE International, 2025. https://doi.org/10.4271/2025-01-7149.

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<div class="section abstract"><div class="htmlview paragraph">Efficient maintenance of highway electromechanical equipment is crucial for ensuring reliability within intelligent highway infrastructure and optimizing the allocation of limited maintenance resources. Traditional Remaining Useful Life (RUL) prediction models frequently face limitations due to the complex and dynamic operating conditions of such systems, which often hinder their predictive accuracy and adaptability. To overcome these persistent challenges, this study introduces an advanced RUL prediction model that inte
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Nagata, H., S. Nomura, K. Oda, T. Kokawa, and K. Yasunaga. "ANALYSIS OF THE FUNCTIONAL ROLE OF PLATELET MEMBRANE GLYCOPROTEINS WITH MONOCLONAL ANTIBODIES." In XIth International Congress on Thrombosis and Haemostasis. Schattauer GmbH, 1987. http://dx.doi.org/10.1055/s-0038-1643509.

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Eight monoclonal antibodies were obtained which recognized platelet surface antigens of these, 5 (NNKYl-32, NNKY2-5, NNKY2-6, NNKY2-11, NNKY2-18 ) recognized GP IIb-IIIa complex, 2 (NNKY5-4, NNKY5-5 ) recognized GP lb and 1 (NNKYl-19) recognized CD 9 antigen. They were used to research the platelet membrane antigens.Monoclonal antibodies that recognize CD 9 antigen, which exists on the surface of platelets, acute lymphoblastic leukemia cells, eosinophils and other tissue, are known to act as an aggregating agent to platelets and NNKYl-19 was fond to induce platelet aggregation accompanied by A
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Reports on the topic "Calcein indicator"

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McLemore, Virginia T., Nels Iverson, Snir Woodard, et al. Geology and Mineral Deposits of the Cornudas Mountains, Otero County, New Mexico. New Mexico Bureau of Geology and Mineral Resources, 2022. http://dx.doi.org/10.58799/ofr-619.

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Re-examination of the rare earth elements (REE) deposits in the Cornudas Mountains is warranted in light of today's economic importance of critical minerals, including REE that are essential in most of our electronic devices. New mapping, petrography, 40Ar/39Ar geochronology, and geochemical analyses have provided a better understanding of the emplacement of these intrusions and associated mineral deposits. The Cornudas Mountains form the northern Trans-Pecos alkaline magmatic province in the southern part of the North American Cordilleran alkaline-igneous belt. The igneous rocks in the Cornud
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Litaor, Iggy, James Ippolito, Iris Zohar, and Michael Massey. Phosphorus capture recycling and utilization for sustainable agriculture using Al/organic composite water treatment residuals. United States Department of Agriculture, 2015. http://dx.doi.org/10.32747/2015.7600037.bard.

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Objectives: 1) develop a thorough understanding of the sorption mechanisms of Pi and Po onto the Al/O- WTR; 2) determine the breakthrough range of the composite Al/O-WTR during P capturing from agro- wastewaters; and 3) critically evaluate the performance of the composite Al/O-WTR as a fertilizer using selected plants grown in lysimeters and test-field studies. Instead of lysimeters we used pots (Israel) and one- liter cone-tainers (USA). We conducted one field study but in spite of major pretreatments the soils still exhibited high enough P from previous experiments so no differences between
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