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Journal articles on the topic 'Calcein indicator'

Author: Grafiati

Published: 5 June 2025

Last updated: 1 August 2025

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1

HUITINK, G. "Constant Calcein Blue: An indicator for spectrofluorometric calcium determination." Talanta 34, no. 4 (1987): 423–26. http://dx.doi.org/10.1016/0039-9140(87)80064-4.

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2

Shyichuk, Alexander, Dorota Ziółkowska, Jan Lamkiewicz, and Maria Kowalska. "Calcium Determination by Complexometric Titration with Calcein Indicator Using Webcam for Endpoint Detection." Water 17, no. 12 (2025): 1757. https://doi.org/10.3390/w17121757.

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Precise and convenient analytical methods are needed for the quantitative determination of calcium in water and food. Complexometric titration remains a reliable technique to determine calcium in milligram amounts. The titrations have been performed automatically by detecting color transitions with a webcam. Classical complexometric indicator calcein provided a sharp color transition. In diffuse reflection mode, the color appearance parameter (Hue) provides better precision and is more resistant to ambient light fluctuations compared to RGB primaries. In fluorescence mode with LED illumination

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DEMOTT, B. J. "Determination of Calcium in Cottage Cheese by Titration." Journal of Food Protection 51, no. 10 (1988): 802–3. http://dx.doi.org/10.4315/0362-028x-51.10.802.

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The calcium concentration of cottage cheese has been determined by the use of disodium ethylene diaminetetraacetate as a calcium binder and calcein as an indicator. This method of analysis compares favorably with the atomic absorption procedure.

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Nieminen, A. L., A. K. Saylor, S. A. Tesfai, B. Herman, and J. J. Lemasters. "Contribution of the mitochondrial permeability transition to lethal injury after exposure of hepatocytes to t-butylhydroperoxide." Biochemical Journal 307, no. 1 (1995): 99–106. http://dx.doi.org/10.1042/bj3070099.

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We have developed a novel method for monitoring the mitochondrial permeability transition in single intact hepatocytes during injury with t-butylhydroperoxide (t-BuOOH). Cultured hepatocytes were loaded with the fluorescence probes, calcein and tetramethylrhodamine methyl ester (TMRM). Depending on loading conditions, calcein labelled the cytosolic space exclusively and did not enter mitochondria or it stained both cytosol and mitochondria. TMRM labelled mitochondria as an indicator of mitochondrial polarization. Fluorescence of two probes was imaged simultaneously using laser-scanning confoca

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Dissard, Delphine, Gernot Nehrke, Gert Jan Reichart, Julius Nouet, and Jelle Bijma. "Effect of the fluorescent indicator calcein on Mg and Sr incorporation into foraminiferal calcite." Geochemistry, Geophysics, Geosystems 10, no. 11 (2009): n/a. http://dx.doi.org/10.1029/2009gc002417.

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Slayman, C. L., V. V. Moussatos, and W. W. Webb. "Endosomal accumulation of pH indicator dyes delivered as acetoxymethyl esters." Journal of Experimental Biology 196, no. 1 (1994): 419–38. http://dx.doi.org/10.1242/jeb.196.1.419.

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Intracellular distributions of the putative cytosolic pH indicator dyes BCECF [2',7'-bis-(2-carboxyethyl)-5(and 6)-carboxyfluorescein], C.SNARF [5(and 6)-carboxy-seminaphthorhodafluor-1], and C.SNARF-calcein have been examined in Neurospora crassa and in murine fibroblasts (NIH-3T3 cells) under conditions in which both kinds of cells produce visible microscopic vacuoles. All three dyes were administered in electroneutral forms, with the hydroxyl and carboxyl groups esterified (designated as -AM esters). As judged qualitatively from fluorescence levels, hydrolytic derivatives of the two heavily

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BUSTOS, PATRICIA L., ALINA E. PERRONE, NATALIA MILDUBERGER, MIRIAM POSTAN, and JACQUELINE BUA. "Oxidative stress damage in the protozoan parasite Trypanosoma cruzi is inhibited by Cyclosporin A." Parasitology 142, no. 8 (2015): 1024–32. http://dx.doi.org/10.1017/s0031182015000232.

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SUMMARYCyclosporin A (CsA) specifically inhibits the mitochondrial permeability transition pore (mPTP). Opening of the mPTP, which is triggered by high levels of matrix [Ca2+] and/or oxidative stress, leads to mitochondrial dysfunction and thus to cell death by either apoptosis or necrosis. In the present study, we analysed the response of Trypanosoma cruzi epimastigote parasites to oxidative stress with 5 mm H2O2, by studying several features related to programmed cell death and the effects of pre-incubation with 1 μm of CsA. We evaluated TcPARP cleavage, DNA integrity, cytochrome c transloca

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Mandavilli, Bhaskar S., Daniel Beacham, Yi-Zhen Hu, Jongtae Yang, and Aimei Chen. "New generation of fluorescent probes for cell-based measurements of caspase Activation and mitochondrial superoxide." Journal of Immunology 210, no. 1_Supplement (2023): 153.17. http://dx.doi.org/10.4049/jimmunol.210.supp.153.17.

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Abstract Cell health and stress readouts are critical indicators of altered or impaired function in normal and diseased states of cells, and work has been underway to develop improved small molecule sensor dyes compatible with traditional imaging and High Content Analysis (HCA) interrogation of apoptotic and mitochondrial stress pathways. The CellEvent™ Caspase Green dye effectively reports caspase activation, but suffers complications in assay configuration when attempting to multiplex with the Green Fluorescent Protein (GFP), calcein, or other 488 laser line tools in fluorescence microscopy.

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Mandavilli, Bhaskar S., Daniel Beacham, Yi Zhen Hu, Jongtae Yang, and Aimei Chen. "Abstract 4781: New generation of fluorescent probes for cell-based measurements of caspase activation and mitochondrial superoxide." Cancer Research 83, no. 7_Supplement (2023): 4781. http://dx.doi.org/10.1158/1538-7445.am2023-4781.

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Abstract Cell health and stress readouts are critical indicators of altered or impaired function in normal and diseased states of cells, and work has been underway to develop improved small molecule sensor dyes compatible with traditional imaging and High Content Analysis (HCA) interrogation of apoptotic and mitochondrial stress pathways. The CellEvent™ Caspase Green dye effectively reports caspase activation, but suffers complications in assay configuration when attempting to multiplex with the Green Fluorescent Protein (GFP), calcein, or other 488 laser line tools in fluorescence microscopy.

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Grabicoski, Edilaine Mauricia Gelinski, David de Souza Jaccoud-Filho, David Lee, Luciane Henneberg, and Marcos Pileggi. "Real-Time Quantitative and Ion-Metal Indicator LAMP-Based Assays for Rapid Detection of Sclerotinia sclerotiorum." Plant Disease 104, no. 5 (2020): 1514–26. http://dx.doi.org/10.1094/pdis-07-19-1455-re.

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Sclerotinia sclerotiorum is one of the most devastating and cosmopolitan plant pathogens. Rapid detection of S. sclerotiorum can provide growers an advantage in knowing what control measures should be taken to minimize crop damage and financial losses caused by it. Loop-mediated isothermal amplification (LAMP) is a fast, sensitive, and specific nucleic acid amplification method that does not require a thermal cycler. This study aimed to develop a LAMP-based assay for the specific detection of S. sclerotiorum (Ss-LAMP). A real-time quantitative LAMP reaction (Ss-qLAMP) and a calcein ion indicat

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Clarkson, Allen B., David Turkel-Parrella, Jonathan H. Williams, Lung Chi Chen, Terry Gordon, and Salim Merali. "Action of Deferoxamine againstPneumocystis carinii." Antimicrobial Agents and Chemotherapy 45, no. 12 (2001): 3560–65. http://dx.doi.org/10.1128/aac.45.12.3560-3565.2001.

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ABSTRACT We found earlier that deferoxamine (DFO), a drug used for treatment of iron overload, is active against a rat model of Pneumocystis carinii pneumonia (PCP). We had assumed a mode of action by deprivation of nutritional iron; however, data here show that DFO penetrates P. carinii, causing irreversible damage, thus indicating a different mode of action. Penetration was demonstrated by showing DFO uptake by high-pressure liquid chromatography analysis. By using calcein-AM as an indicator, exposure to DFO was shown to cause a reduction inP. carinii cytoplasmic free iron. Exposure to ≥100

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Perez, A., C. Abele, P. Pierozan, A. Höglund, O. Karlsson, and M. Breitholtz. "P22-11 An Image-based toxicity screening method: Calcein AM as an indicator of esterase activity in Daphnia magna to detect sublethal effects in vivo." Toxicology Letters 399 (September 2024): S339. http://dx.doi.org/10.1016/j.toxlet.2024.07.808.

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Ferrera-González, Juan, Laura Francés-Soriano, Cristina Galiana-Roselló, et al. "Initial Biological Assessment of Upconversion Nanohybrids." Biomedicines 9, no. 10 (2021): 1419. http://dx.doi.org/10.3390/biomedicines9101419.

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Nanoparticles for medical use should be non-cytotoxic and free of bacterial contamination. Upconversion nanoparticles (UCNPs) coated with cucurbit[7]uril (CB[7]) made by combining UCNPs free of oleic acid, here termed bare UCNPs (UCn), and CB[7], i.e., UC@CB[7] nanohybrids, could be used as photoactive inorganic-organic hybrid scaffolds for biological applications. UCNPs, in general, are not considered to be highly toxic materials, but the release of fluorides and lanthanides upon their dissolution may cause cytotoxicity. To identify potential adverse effects of the nanoparticles, dehydrogenas

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Doan, Lisa V., Olga Eydlin, Boris Piskoun, et al. "Despite Differences in Cytosolic Calcium Regulation, Lidocaine Toxicity Is Similar in Adult and Neonatal Rat Dorsal Root Ganglia In Vitro." Anesthesiology 120, no. 1 (2014): 50–61. http://dx.doi.org/10.1097/aln.0b013e3182a2a561.

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Abstract Background: Neuraxial local anesthetics may have neurological complications thought to be due to neurotoxicity. A primary site of action of local anesthetics is the dorsal root ganglia (DRG) neuron. Physiologic differences have been noted between young and adult DRG neurons; hence, the authors examined whether there were any differences in lidocaine-induced changes in calcium and lidocaine toxicity in neonatal and adult rat DRG neurons. Methods: DRG neurons were cultured from postnatal day 7 (P7) and adult rats. Lidocaine-induced changes in cytosolic calcium were examined with the cal

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15

Абакушина, Е. В., Ю. В. Гельм та А. С. Миценык. "Флуоресцентный микроскопический анализ жизнеспособности ооцитов млекопитающих после витрификации-=SUP=-*-=/SUP=-". Журнал технической физики 126, № 5 (2019): 611. http://dx.doi.org/10.21883/os.2019.05.47660.9-19.

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AbstractThe paper reports the results of a fluorescent microscopy analysis of the viability of oocytes from cattle and pigs after vitrification. Oocytes were frozen in a vitrification media containing varying concentrations of cryoprotectors in several steps with subsequent vitrification. After cryobank storage for 14 days, experimental samples were thawed and oocyte viability was analyzed by oocyte morphology assessment and fluorescent microscopy. Two different kits were used to stain oocytes, one specific for necrosis/apoptosis (Propidium iodide/Alexa Fluor 488 Annexin) and the other specifi

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Bonifacio, Massimiliano, Antonella Rigo, Angela Bonalumi та ін. "The Sesquiterpene Oil α-Bisabolol Induces Apoptosis of B-Chronic Lymphocytic Leukemia Primary Cells". Blood 118, № 21 (2011): 1319. http://dx.doi.org/10.1182/blood.v118.21.1319.1319.

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Abstract Abstract 1319 We have recently demonstrated that the sesquiterpene oil α-bisabolol is cytotoxic against primary acute leukemia cells ex vivo and in chronic myeloid leukemia cell lines. It enters cells via lipid rafts and activates the mitochondrial-dependent intrinsic pathway of apoptosis, exerting a preferential toxicity against malignant vs normal cells probably due to their higher content in lipid rafts. Here we investigated the in vitro activity of α-bisabolol in primary cells from patients with B-Chronic Lymphocytic Leukemia (B-CLL). Twenty-six patients with newly diagnosed B-CLL

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Blaz, T. "Polypyrrole–calcion film as a membrane and solid-contact in an indicator electrode for potentiometric titrations." Talanta 52, no. 2 (2000): 319–28. http://dx.doi.org/10.1016/s0039-9140(00)00363-5.

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Mourad, M., J. Malaise, R. C. Chautems, G. Hanique, and J. P. Squifflet. "Early posttransplant calcemia as a predictive indicator for parathyroidectomy in kidney allograft recipients with tertiary hyperparathyroidism." Transplantation Proceedings 32, no. 2 (2000): 437–40. http://dx.doi.org/10.1016/s0041-1345(00)00841-1.

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Yu, Tengteng, Lijie Xing, Liang Lin, et al. "An Immune Based, Anti-CD138 Targeting Antibody for the Treatment of Multiple Myeloma." Blood 132, Supplement 1 (2018): 5617. http://dx.doi.org/10.1182/blood-2018-99-119112.

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Abstract CD138 (Syndecan-1), a member of integral membrane family of heparan sulfate proteoglycans (HSPGS), is highly expressed on differentiated plasma cells (PC) and is both a primary diagnostic biomarker of multiple myeloma (MM) as well as an indicator of poor clinical prognosis. This surface antigen is an attractive candidate for targeted immunotherapy for MM, given its constitutive expression during disease progression, including smoldering myeloma, a relatively early asymptomatic phase of disease that is potentially amenable to early treatment. We here investigated the targeted use of ch

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Marschke, Keith, Eric Vajda, and Lin Zhi. "Novel Small-Molecule Human G-CSF Receptor Agonist Stimulates Neutrophil Counts In Monkeys and Displays Anti-Proliferative Effects On Tumor Cells Mediated By a Reduction Of Intracellular Iron." Blood 122, no. 21 (2013): 3462. http://dx.doi.org/10.1182/blood.v122.21.3462.3462.

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Abstract Previously we reported on the discovery of a series of novel, non-peptidyl, small molecules that selectively activate the human granulocyte colony stimulating factor receptor (hG-CSFR) in a manner distinct from G-CSF and similar to the mechanism of small-molecule human thrombopoietin receptor (hTPOR) agonists [Marschke KB, et al. ASH 53rd Annual Meeting Abstracts, Blood 2011 118(21):1453, Abstract 3391]. The lead compound, LG7455, activates STAT5 and STAT3 signal transduction and induces G-CSF-dependent hematopoietic cell growth, but not TPO-dependent or erythropoietin-dependent cell

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Vishnevskii, K. A., E. V. Parshina, A. Yu Zemchenkov, R. P. Gerasemchuk, I. A. Reutsky, and A. N. Belskikh. "Analysis of patient's survival on renal replacement therapy in a large city: a multicenter prospective cohort observational study." Nephrology and Dialysis 27, no. 1 (2025): 59–79. https://doi.org/10.28996/2618-9801-2025-1-59-79.

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A direct comparison of the long-term effectiveness of combined interventions in controlled studies is often challenging, if not infeasible due to ethical concerns, due to ethical concerns, including the reluctance to withhold potentially beneficial treatments. This gap can be addressed through pragmatic research or the analysis of large prospectively collected datasets, such as registers. In addition providing valuable outcome assessments, these datasets enable the formation of matched groups for comparison in interventional studies, which, under certain conditions, may serve as a form of quas

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Haider, Nazia, Ghansham ., Zahid Mahmood, Bilal Burki, and Rizwan Karwar. "Early Bone Pain Relief a Reliable Indicator of Successful Para Thyroidectomy - A Comparative Prospective Study." Pakistan Journal of Medical and Health Sciences 17, no. 1 (2023): 55–57. http://dx.doi.org/10.53350/pjmhs202317155.

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Aim: To evaluate early bone pain relief as anindicatorof successful Para thyroidectomy in hyperparathyroidism. Methodology: A prospective, comparative study was conducted at the Department of Surgery, Jinnah Postgraduate Medical Center, between March 2022 and August 2022. All patients with symptoms of hyperparathyroidism, confirmed with increased intact parathyroid hormone and elevated levels of calcium onpresentation were included in the study. Patients were classified as primaryhyperparathyroidism when the MIBI scan detected an adenoma with clinical features ofhypercalcemia and increased PTH

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Foster, B. A., F. A. Diaz, P. T. Hardin, E. J. Gutierrez, and K. R. Bondioli. "202 CHARACTERIZATION OF BOVINE OOCYTE CYTOPLASMIC MATURATION WITH COMMON IN VITRO MATURATION PROTOCOLS." Reproduction, Fertility and Development 28, no. 2 (2016): 232. http://dx.doi.org/10.1071/rdv28n2ab202.

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Modulators of 3′-5′-cyclic adenosine monophosphate have been extensively researched to delay nuclear maturation in in vitro maturation (IVM) systems to improve synchronization of nuclear and cytoplasmic maturation. However while normal maturation for many organelles has been characterised, there is a lack of information on how modulators affect cytoplasmic maturation. The goal of this study was to identify the effect of different components of bovine oocyte maturation systems on 3 aspects of cytoplasmic maturation. Bovine oocytes were collected from mixed breed beef cattle using transvaginal u

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Chambi Condori, Pedro Pablo. "Diversificación de carteras de inversión con criptomonedas." Quipukamayoc 29, no. 60 (2021): 51–60. http://dx.doi.org/10.15381/quipu.v29i60.20471.

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Objetivo: Organizar en forma experimental carteras de inversión con los criptoactivos bajo la teoría moderna de estructuración de portafolios de inversión de Markowitz. Método: La investigación fue del tipo cuantitativa y experimental, abarcando el proceso de estructuración de portafolios de inversión con criptomonedas bajo la teoría de Markowitz, mediante el modelo de programación no lineal cuantitativa. Resultados: Se pudo observar que el periodo de cotización más pronunciado se presentó en el periodo comprendido entre 2017 a 2018. Una rentabilidad mayor le corresponde a Ethereum, seguido de

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Lei, Pan, Zhiyang Li, Qiuwei Hua та ін. "Ursolic Acid Alleviates Neuroinflammation after Intracerebral Hemorrhage by Mediating Microglial Pyroptosis via the NF-κB/NLRP3/GSDMD Pathway". International Journal of Molecular Sciences 24, № 19 (2023): 14771. http://dx.doi.org/10.3390/ijms241914771.

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The neuroinflammatory response after intracerebral hemorrhage (ICH) causes a large amount of neuronal loss, and inhibiting the inflammatory response can improve the prognosis. In previous laboratory studies and clinical trials, ursolic acid (UA) inhibited the inflammatory response, but whether it can be administered to inhibit the neuroinflammatory response after cerebral hemorrhage is unknown. The aim of this study was to investigate the effects of ursolic acid after cerebral hemorrhage. Online databases were used to obtain potential therapeutic targets of ursolic acid for the treatment of ce

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Riaño, Carlos, Alejandro Echavarría, and Antonio Noreña. "Hidroxiapatita sintética de porosidad inducida-comparación con el hueso calcinado-." Revista Facultad de Ingeniería Universidad de Antioquia, no. 19 (October 30, 1999): 56–64. http://dx.doi.org/10.17533/udea.redin.325833.

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La hidroxiapatita (HAP), el compuesto mineral de los hue os, puede obtenerse sintéticamente por reacción entre cantidades estequiornétricas de soluciones acuosas de fosfato de amonio y nitrato de calcio en condiciones básicas. El precipitado se somete a un doble lavado con agua destilada y se le incorpora fibras de celulosa (papel de filtro analítico molido) para obtener la porosidad deseada, en cantidades de 8, 16, 24 y 32%. Luego de filtrado. se seca y se calcina a 1050 ºC por 2.5h.Tanto el producto obtenido corno mue. tras de hueso de canino cortical calcinado a 1050 ºC fueron caracterizad

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Wang, Yao, Xiao Li, Chuanguo Yan, Liuwei Xie, and Yang Yang. "Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells." Metabolites 13, no. 12 (2023): 1173. http://dx.doi.org/10.3390/metabo13121173.

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Renal failure is a common chronic disease in dogs that substantially affects both their quality of life and longevity. The objective of this study was to assess the protective mechanisms of baicalin in cisplatin-induced Madin–Darby canine kidney (MDCK) epithelial cells’ apoptosis model and explore the impacts of baicalin at varying doses on various indexes, such as cisplatin-induced MDCK cell apoptosis, oxidation and antioxidation, and inflammatory factors. (Methods) MDCK cells in the logarithmic growth phase were randomly divided into a control group, a model group (20 μmol/L cisplatin), and

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Mamedova, E. O., O. O. Golounina, and Z. E. Belaya. "Albumin adjustment of total serum calcium – is it worth doing?" Problems of Endocrinology 70, no. 6 (2024): 45–61. https://doi.org/10.14341/probl13503.

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Calcium is the most abundant mineral in the human body. About 99% of calcium is deposited in the bones in the form of hydroxyapatite and only 1% is located in the intracellular and extracellular fluid. Ionized calcium, which makes up about 50% of the total amount of circulating calcium, is biologically active; the remaining percentage is bound to plasma proteins (40%, of which albumin accounts for 90%, and globulins for 10%), or is in complex with anions (10%) such as citrate, lactate, bicarbonate, phosphate. Hypo- and hypercalcemia are common conditions treated by physicians of various specia

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Godovanets, O. I., O. O. Vitkovskyi, M. P. Mytchenok, and T. S. Kitsak. "CLINICAL EFFECTIVENESS OF COMPREHENSIVE TREATMENT FOR ACUTE ODONTOGENIC PERIOSTITIS IN CHILDREN WITH CONCOMITANT DIFFUSE NON-TOXIC GOITR." Актуальні проблеми сучасної медицини: Вісник Української медичної стоматологічної академії 25, no. 2 (2025): 116–21. https://doi.org/10.31718/2077-1096.25.2.116.

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Objective. The aim of this study was to evaluate the effectiveness of treating acute odontogenic periostitis in children with diffuse toxic goiter using a therapeutic and preventive regimen designed to correct micronutrient and vitamin deficiencies, enhance antioxidant system function, and boost local immunity, with the goal of improving overall treatment outcomes. Material and methods. To evaluate the effectiveness of the proposed treatment method for acute odontogenic periostitis in children with concomitant diffuse non-toxic goiter, a total of 38 patients were enrolled and divided into two

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Acosta, Luciana Paola, Martin Eduardo Richardet, Matias Molina, and Eduardo Richardet. "Analysis of lymphocyte-neutrophil relationship in patients with advanced kidney cancer and the response to one-line treatment with target therapies." Journal of Clinical Oncology 35, no. 15_suppl (2017): e23034-e23034. http://dx.doi.org/10.1200/jco.2017.35.15_suppl.e23034.

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e23034 Background: In kidney cancer, some important prognostic factors of survival are known and inflammation plays a key role. It has been proven that cancer progress is not only determined by the characteristics of the tumor but also by the response of the host. It is known that a high RNL has been identified as an independent prognostic factor associated to poor survival in several/different types of cancer, including breast cancer, colon cancer, gastric cancer, mesothelioma and pancreas. The primary aim was to evaluate if RNL can be used as a prognostic indicator of SLP in patients with ad

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Golovanov, S. A., M. Yu Prosyannikov, A. V. Sivkov, N. V. Anokhin, D. A. Voytko, and V. V. Drozhzheva. "Metabolic risk factors and urinary stones formation. VII: Uricosuria lithogenic features in men and women." Experimental and Сlinical Urology 16, no. 3 (2023): 154–64. http://dx.doi.org/10.29188/2222-8543-2023-16-3-154-164.

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Introduction. Hyperuricosuria usually considered as the main metabolic factor in the formation of uric acid stones, but this issue remains unclear. Gender differences in the prevalence of urolithiasis (UL) indicate the need for a more detailed study of the lithogenic properties of uricosuria and its role in the development of various metabolic types of UL. In this study, was investigated the effect of varying degrees of uricosuria in men and women with UL on metabolic parameters and the frequency of detection of urinary stones of different chemical composition. Materials and methods. A total o

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Hough, Rebecca F., Galina A. Gusarova, Mohammad N. Islam, and Jahar Bhattacharya. "Alveolar Acid Injury Causes Endothelial Mitochondrial Depolarization." FASEB Journal 30, S1 (2016). http://dx.doi.org/10.1096/fasebj.30.1_supplement.982.5.

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Lung inflammation and edema, the characteristic features of Acute Lung Injury (ALI), result from lung microvascular barrier deterioration. Since ALI associates with loss of mitochondrial function, we evaluated endothelial mitochondrial responses to alveolar acid instillation. We established isolated, blood‐perfused mouse lungs at constant pulmonary artery, left atrial and alveolar pressures (n=4). To view alveoli and capillaries by confocal microscopy, we micropunctured alveoli to load the cytosolic fluorescent dyes, calcein green (CG) and calcein red (CR), respectively. We loaded capillaries

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Haeri, Bahareh, and Paul M. M. Weers. "Binding Interaction of Lipid‐bound ApoA–I with Lipopolysaccharides and Phosphatidylglycerol." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.630.14.

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Apolipoprotein A‐I (apoA‐I) is a major component of high‐density lipoprotein (HDL), an anti‐antherogenic complex responsible for reverse cholesterol transport from peripheral tissues to liver, and mediates several essential metabolic functions related to heart disease. Human apoA‐I is 28 kDa protein found in lipid‐free and lipid‐bound forms. Previous studies have shown that lipid‐free apoA‐I binds and neutralizes lipopolysaccharides (LPS), which are major constituents of the outer bacterial membrane of gram‐negative bacteria, therefore reducing endotoxic effects. In addition, the protein desta

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Wall-Palmer, Debbie, Lisette Mekkes, Paula Ramos-Silva, et al. "The impacts of past, present and future ocean chemistry on predatory planktonic snails." August 4, 2021. https://doi.org/10.1098/rsos.202265.

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The atlantid heteropods represent the only predatory, aragonite shelled zooplankton. Atlantid shell production is likely to be sensitive to ocean acidification (OA), and yet we know little about their mechanisms of calcification, or their response to changing ocean chemistry. Here, we present the first study into calcification and gene expression effects of short-term OA exposure on juvenile atlantids across three pH scenarios: mid- 1960s, ambient and 2050 conditions. Calcification and gene expression indicate a distinct response to each treatment. Shell extension and shell volume were reduced

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Aoyagi, Minoru. "Fluorescence microscope observation of the structure of a calcium alginate hydrogel." Journal of Polymer Engineering, March 7, 2022. http://dx.doi.org/10.1515/polyeng-2021-0284.

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Abstract Calcium alginate hydrogels are used in a wide range of applications in the food, medical, pharmaceutical, and cosmetic industries. I have studied a calcium alginate hydrogel as an ultrasound phantom material. This hydrogel is formed using sodium alginate, calcium sulfate dihydrate, trisodium phosphate 12-hydrate, glycerol, and water, and mimics the ultrasound properties of human soft tissue. In this study, the structure of the calcium alginate hydrogel was observed with a fluorescence microscope after staining with the calcium indicator calcein. Two types of hydrogel structures, tape-

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Liu, Xuemeng, Xiaotian Liu, Shani Chen, et al. "Calcium leakage involved in nematotoxic effects of the Conidiobolus obscurus CytCo protein on the pine wood nematode, Bursaphelenchus xylophilus." Pest Management Science, August 7, 2024. http://dx.doi.org/10.1002/ps.8365.

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AbstractBACKGROUNDThe pine wood nematode Bursaphelenchus xylophilus, a severe invasive species, is responsible for causing widespread pine wilt disease. The CytCo protein, a pore‐forming toxin derived from Conidiobolus obscurus, exhibits nematotoxicity towards B. xylophilus.RESULTSOur present study reveals the expression variation of a range of gene products in B. xylophilus that respond to the effects of CytCo using the isobaric tags for relative and absolute quantification proteomics technology. Functional enrichment analysis indicates that many differentially expressed proteins are linked t

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Gao, Jin, Xi Xie, Xiang-Feng Liu, et al. "Efficacy of calcein as a chemical marker of Potamocorbula laevis." Frontiers in Marine Science 11 (July 26, 2024). http://dx.doi.org/10.3389/fmars.2024.1379571.

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IntroductionCalcein was used to develop a shell marking method for Potamocorbula laevis.MethodsThe suitable conditions for marking were investigated, including marking concentration, immersion time, and water temperature. The impacts and feasibility of the marking method were assessed based on the survival rate of P. laevis, the success rate of fluorescence marking, marking quality, and alterations in activities of antioxidant enzymes in the digestive gland of the experimental bivalves. Two concentrations of calcein (20 and 50 mg/L) were used and the immersion time included 1 and 2 h, respecti

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Gordan, Richard, Nadezhda Fefelova, Zhenhang Zhao, and Lai-Hua Xie. "Abstract 19344: Involvement of Mitochondrial Permeability Transition Pore (mPTP) in Cardiac Arrhythmias: Evidence From Cyclophilin D Knockout Mice." Circulation 132, suppl_3 (2015). http://dx.doi.org/10.1161/circ.132.suppl_3.19344.

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Our previous studies have demonstrated that mitochondrial membrane potential ( ΔΨ m ) dissipation leads to mitochondrial Ca efflux via the mitochondrial permeability transition pore (mPTP), and consequently promotes spontaneous Ca waves (CaW) in isolated cardiomyocytes. In the present study, we have used a genetic mouse model which lacks cyclophilin D (CypD KO), a necessary regulator for mPTP opening, to assess the cardioprotective effect of mPTP inhibition on cellular CaWs, Ca alternans, and whole-heart inducible arrhythmias. Ventricular myocytes were isolated from wild type (WT) and CypD KO

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Dmitriev, Ruslan I., Irina A. Okkelman, Tara Foley, and Dmitri B. Papkovsky. "Live cell microscopy of intestinal organoid oxygenation." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.590.1.

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Oxygenation of stem cell niche is important metabolic activity indicator for the intestine in normal and diseased states. In vivo, it can be affected by such factors as diet, drugs, metabolites produced by microbiota, and inflammation. We hypothesized that O2 must be also very important for adequate development of organoids and organs‐on‐chip, growing as 3D cultures. We took advantage of cell‐penetrating O2‐sensitive probes and phosphorescence lifetime imaging microscopy (PLIM), which, in contrast to the end‐point hypoxia stains and electrode‐based measurements, provides high‐resolution real‐t

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Shaji, Chinchusha Anasooya, Bobby Robinson, Antonia Yeager, Madhava Beeram, Claire L. Isbell, and Binu Tharakan. "A tri‐phasic role for hydrogen peroxide in blood‐brain barrier endothelial cells." FASEB Journal 31, S1 (2017). http://dx.doi.org/10.1096/fasebj.31.1_supplement.834.3.

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In microvascular endothelial cells, hydrogen peroxide (H2O2) plays an important role as a signaling second messenger as well as an inducer of oxidative stress in various pathologies such as ischemic and traumatic injuries. However, it is unclear how H2O2 influences various cellular functions differentially, particularly in microvascular endothelial cells of the blood‐brain barrier (BBB). We hypothesized that the change in cellular concentrations of H2O2 is a major contributor in regulation of angiogenesis, endothelial cell permeability and cell death/apoptosis. Further, we postulate that these

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Mendoza, Diego, Roberto Flores, Nadia Lara, and Constanza MAchin. "Analysis of the Recovery Process of the Calcine Contained in Damaged Windshields under the Principles of Circular Economy." Ingenieria y Universidad 28 (June 2, 2024). https://doi.org/10.11144/javeriana.iued28.arpc.

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The Circular Economy (CE) plays a fundamental role in the vigor of sustainability. Nowadays, institutions and governments have been looking for alternatives to achieve their objectives of reducing the generation and disposal of pollutants. In this work, it is analyzed the process of recovering calcine by a delamination process of damaged windshields in a recycling company. Three tools focused on the EC are used in this study: The Material Circularity Indicator (MCI), the Circular Economy Toolkit (CET) and the Life Cycle Assessment (LCA). Currently, the company has a delamination process for re

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Schallmoser, Andreas, Rebekka Einenkel, Cara Färber, Norah Emrich, Julia John, and Nicole Sänger. "The effect of high-throughput vitrification of human ovarian cortex tissue on follicular viability: a promising alternative to conventional slow freezing?" Archives of Gynecology and Obstetrics, September 29, 2022. http://dx.doi.org/10.1007/s00404-022-06797-6.

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Abstract Background The standard procedure most frequently used for ovarian tissue cryopreservation (OTC) is slow freezing, while vitrification has been proposed as promising alternative and has built an impressive catalog of success in fertility laboratories regarding cryopreservation of oocytes and embryos. Methods We developed and evaluated a high-throughput protocol for vitrification of human ovarian tissue suitable for clinical processing. Follicular viability was assessed via calcein staining prior and after cryopreservation analyzing ovarian tissue of a cohort of 30 patients. Results We

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Vitkovskyi, O. O. "Clinical evaluation of the treatment complex of acute odontogenic periostitis in children on the base of diffused non-toxic goiter." Medicine Today and Tomorrow 91, no. 1 (2022). http://dx.doi.org/10.35339/msz.2022.91.1.vit.

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Inflammatory diseases in the maxillo-facial region in children occupy a leading place in the work of a polyclinic doctor. At the same time, their diagnosis is imperfect and they often occur on a premorbid background. The clinical features of acute odontogenic periostitis against the background of thyroid pathology in children were studied to improve the effectiveness of treatment. We examined 38 children, formed two groups of observations. Children of both groups were given generally accepted treatment. Children of the main group were additionally prescribed means "Imupret" and "Calcemin advan

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Leon, Brandon, Sarvesh Chelvanambi, Rabab Rabab ElMergawy, Moraima Noda, Bernhard Maier, and Matthias A. Clauss. "Assessment of T cell chemotaxis to S1P." Proceedings of IMPRS 4, no. 1 (2021). http://dx.doi.org/10.18060/25859.

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Introduction: Migration of leukocytes in response to chemical gradients, chemotaxis, is dependent on many factors, including cell type, surface markers, the chemoattractant, etc. Sphinogsine-1-phosphate (S1P) is a chemoattractant playing a large role in migrating activated T cells out of lymph nodes by binding to their S1P receptor, S1P1. The importance of the egress in T cells from lymph nodes is highlighted by pharmacological disruption of this migration can lead to immune dampening and thus control of multiple sclerosis, an autoimmune disease. In the case of human immunodeficiency virus (HI

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O.P., Chzhu, and Araviashvili D.E. "SCREENING INVESTIGATIONS OF VITAMIN D CONTENT DIFFERENT POPULATION GROUPS OF BIG SOCHI." "Medical & pharmaceutical journal "Pulse", July 30, 2021, 101–7. http://dx.doi.org/10.26787/nydha-2686-6838-2021-23-7-101-107.

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Data on vitamin D metabolism suggest that it is a secosteroid prohormone. Current studies have shown that the mechanism of functioning of vitamin D is associated with a specific receptor of the hormonally active form of this vitamin (VDR). Receptors for vitamin D are identified in more than 40 target tissues of the body (bones, muscles, intestine, kidneys, heart, prostate gland, mammary glands, pancreas), in particular in most cells of the immune system and epithelial cells lining the mucous membranes which determines many of its biological effects. Vitamin D levels affect 172 major physiologi

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