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Journal articles on the topic 'Calcium buffers'

Author: Grafiati

Published: 4 June 2021

Last updated: 8 February 2022

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1

Snow, P., and R. Nuccitelli. "Calcium buffer injections delay cleavage in Xenopus laevis blastomeres." Journal of Cell Biology 122, no. 2 (1993): 387–94. http://dx.doi.org/10.1083/jcb.122.2.387.

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Microinjection of calcium buffers into the two-cell Xenopus laevis embryo delays cell division in a dose-dependent manner. Four calcium buffers in the BAPTA series with different affinities for calcium were used to distinguish between a localized calcium gradient regulating cleavage and the global calcium concentration regulating this event. DibromoBAPTA (Kd = 1.5 microM) was found to delay cleavage at the lowest intracellular concentration (1.3 mM) of the four buffers tested. The effectiveness of the calcium buffers was dependent upon the buffer dissociation constant but not in a linear fashi

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2

Prins, D., and M. Michalak. "Organellar Calcium Buffers." Cold Spring Harbor Perspectives in Biology 3, no. 3 (2011): a004069. http://dx.doi.org/10.1101/cshperspect.a004069.

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3

McMahon, Shane M., Che-Wei Chang, and Meyer B. Jackson. "Multiple cytosolic calcium buffers in posterior pituitary nerve terminals." Journal of General Physiology 147, no. 3 (2016): 243–54. http://dx.doi.org/10.1085/jgp.201511525.

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Cytosolic Ca2+ buffers bind to a large fraction of Ca2+ as it enters a cell, shaping Ca2+ signals both spatially and temporally. In this way, cytosolic Ca2+ buffers regulate excitation-secretion coupling and short-term plasticity of release. The posterior pituitary is composed of peptidergic nerve terminals, which release oxytocin and vasopressin in response to Ca2+ entry. Secretion of these hormones exhibits a complex dependence on the frequency and pattern of electrical activity, and the role of cytosolic Ca2+ buffers in controlling pituitary Ca2+ signaling is poorly understood. Here, cytoso

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4

Speksnijder, J. E., A. L. Miller, M. H. Weisenseel, T. H. Chen, and L. F. Jaffe. "Calcium buffer injections block fucoid egg development by facilitating calcium diffusion." Proceedings of the National Academy of Sciences 86, no. 17 (1989): 6607–11. http://dx.doi.org/10.1073/pnas.86.17.6607.

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The polarity of fucoid eggs is fixed either when tip growth starts or a bit earlier. A steady flow of calcium ions into the incipient tip is thought to establish a high calcium zone that is needed for its localization and formation. To test this hypothesis, we have injected seven different 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA)-type calcium buffers into Pelvetia eggs many hours before tip growth normally starts. Critical final cell concentrations of each buffer prove to block outgrowth (as well as cell division) for up to 2 weeks. This critical inhibitory concentratio

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5

Carrera, Germán, Amparo Gil, Javier Segura, and Bernat Soria. "Software for simulating calcium-triggered exocytotic processes." American Journal of Physiology-Cell Physiology 292, no. 2 (2007): C749—C755. http://dx.doi.org/10.1152/ajpcell.00082.2006.

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We describe a software package for the simulation of exocytotic events from readily releasable pools of secretory vesicles in neuroendocrine cells and presynaptic terminals. The visual package Ca3D_Exolab simulates the entry of Ca2+ through the calcium channels, the kinetic reactions of calcium with buffers, the diffusion of calcium and mobile buffers, and the kinetic reactions of calcium with the secretory vesicles. The location of both channels and secretory vesicles can be set by using a graphical interface. Calcium and buffer concentrations at different depths from the cellular membrane an

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6

Lindsay, H. D., M. J. Whitaker, and C. C. Ford. "Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts." Journal of Cell Science 108, no. 11 (1995): 3557–68. http://dx.doi.org/10.1242/jcs.108.11.3557.

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Activation of p34cdc2 kinase is essential for entry into mitosis while subsequent deactivation and cyclin degradation are associated with exit. In Xenopus embryos, both of these phases are regulated by post-translation modifications and occur spontaneously on incubation of extracts prepared late in the first cell cycle. Even though high levels of calcium buffer were initially used to prepare these extracts, we found that free calcium levels in them remained in the observed physiological range (200-500 nM). Further addition of calcium buffers only slightly reduced free calcium levels, but inhib

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7

Neher, Erwin. "Calcium Buffers in Flash-Light." Biophysical Journal 79, no. 6 (2000): 2783–84. http://dx.doi.org/10.1016/s0006-3495(00)76517-9.

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8

McMahon, Shane M., and Meyer B. Jackson. "An Inconvenient Truth: Calcium Sensors Are Calcium Buffers." Trends in Neurosciences 41, no. 12 (2018): 880–84. http://dx.doi.org/10.1016/j.tins.2018.09.005.

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9

Crowell, J. A., and G. N. Bowers. "Apparent binding of ionized calcium by various buffers." Clinical Chemistry 31, no. 2 (1985): 267–70. http://dx.doi.org/10.1093/clinchem/31.2.267.

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Abstract Using different substance concentrations in an aqueous 1.25 mmol/L solution of CaCl2 plus NaCl to a final solution ionic strength of 160 mmol/L, we tested six buffers for their effect on measurements of ionized calcium (Ca2+). Measured Ca2+ decreased with increasing ionic strength and pH. Increasing concentrations of Tris caused a positive Ca2+ electrode bias; the other five buffers caused a negative Ca2+ electrode bias with increasing concentration. 4-(2-Hydroxyethyl)-1-piperazineethanesulfonic acid ("HEPES") at 10 mmol/L maintained pH 7.39 in the aqueous reference solutions containi

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10

Holt, Carl, D. Thomas Davies, and Andrew J. R. Law. "Effects of colloidal calcium phosphate content and free calcium ion concentration in the milk serum on the dissociation of bovine casein micelles." Journal of Dairy Research 53, no. 4 (1986): 557–72. http://dx.doi.org/10.1017/s0022029900033082.

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SummaryThe strength of binding of the individual caseins and the nature of the bonding within bovine casein micelles were examined through dissociation of the micelles by dialysis of skim milk either against phosphate-free buffers containing 3 or 6 mm-CaCl2, or against buffers that were nearly saturated with respect to micellar calcium phosphate, but which had a free Ca2+ concentration in the range 0·4–5·9 mm. Dissociation was followed by ultracentrifuging the dialysed milks and determining the partition of the total and the individual caseins between the pellet and serum. During dialysis agai

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11

Csernoch, L., V. Jacquemond, and M. F. Schneider. "Microinjection of strong calcium buffers suppresses the peak of calcium release during depolarization in frog skeletal muscle fibers." Journal of General Physiology 101, no. 2 (1993): 297–333. http://dx.doi.org/10.1085/jgp.101.2.297.

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The effects of high intracellular concentrations of various calcium buffers on the myoplasmic calcium transient and on the rate of release of calcium (Rrel) from the sarcoplasmic reticulum (SR) were studied in voltage-clamped frog skeletal muscle fibers. The changes in intracellular calcium concentration (delta[Ca2+]) for 200-ms pulses to 0-20 mV were recorded before and after the injection of the calcium buffer and the underlying Rrel was calculated. If the buffer concentration after the injection was high, the initial rate of rise of the calcium transient was slower after injection than befo

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12

Dave, Devanshi D., and Brajesh Kumar Jha. "Analytically depicting the calcium diffusion for Alzheimer’s affected cell." International Journal of Biomathematics 11, no. 07 (2018): 1850088. http://dx.doi.org/10.1142/s1793524518500882.

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Brain is the most complex structure of the human body. The processes going inside the brain and the mechanisms behind it have been unrevealed up to certain extent only. Out of the various physiological phenomena carried out by the brain, calcium signalling can be considered as one of the most important. Calcium being a second messenger plays an important role in transformation of various information. In view of above, an attempt has been made here to study calcium signalling in presence of buffers, i.e. one kind of proteins and endoplasmic reticulum (ER), which is also known as store house of

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13

Jha, Brajesh Kumar, and Devanshi D. Dave. "Approximation of Calcium Diffusion in Alzheimeric Cell." Journal of Multiscale Modelling 11, no. 02 (2019): 2050001. http://dx.doi.org/10.1142/s1756973720500018.

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Alzheimer’s disease (AD) is the most common form of dementia prevailing worldwide. It has been found that the higher level of cytosolic calcium leads to the pathological symptoms of AD and the two cytoplasmic calcium binding buffers have their roles in sustaining this dementia. However, the area of their functioning and working is found to be different. Calmodulin is found in CA1-hippocampus neurons, whereas calbindin-D28k is found in basal forebrain cholinergic neurons. Based on this physiology, in this research paper an attempt has been made to delineate the physiological phenomenon of calci

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14

Smith, Elizabeth F. "Regulation of Flagellar Dynein by Calcium and a Role for an Axonemal Calmodulin and Calmodulin-dependent Kinase." Molecular Biology of the Cell 13, no. 9 (2002): 3303–13. http://dx.doi.org/10.1091/mbc.e02-04-0185.

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Ciliary and flagellar motility is regulated by changes in intraflagellar calcium. However, the molecular mechanism by which calcium controls motility is unknown. We tested the hypothesis that calcium regulates motility by controlling dynein-driven microtubule sliding and that the central pair and radial spokes are involved in this regulation. We isolated axonemes from Chlamydomonasmutants and measured microtubule sliding velocity in buffers containing 1 mM ATP and various concentrations of calcium. In buffers with pCa > 8, microtubule sliding velocity in axonemes lacking the central apparat

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15

Tsai, Je-Chiang. "Do calcium buffers always slow down the propagation of calcium waves?" Journal of Mathematical Biology 67, no. 6-7 (2012): 1587–632. http://dx.doi.org/10.1007/s00285-012-0605-y.

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16

McGuigan, John A. S., Daniel Lüthi, and Arlette Buri. "Calcium buffer solutions and how to make them: A do it yourself guide." Canadian Journal of Physiology and Pharmacology 69, no. 11 (1991): 1733–49. http://dx.doi.org/10.1139/y91-257.

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In measurements of the intracellular free calcium concentration ([Ca2+]) using either microelectrodes or fluorescent probes, calibration is normally carried out in EGTA calcium buffer solutions. In the first part of the article the general properties of calcium buffer solutions are discussed, the equations used to calculate the apparent calcium binding constant (Kapp) are derived, and the difficulties in the calculation are discussed. The effects of the purity of EGTA as well as the influence of calcium contamination on the buffer solutions are explained. Because of the difficulties in calcula

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17

Neher, E., and G. J. Augustine. "Calcium gradients and buffers in bovine chromaffin cells." Journal of Physiology 450, no. 1 (1992): 273–301. http://dx.doi.org/10.1113/jphysiol.1992.sp019127.

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18

Tiffert, T., and V. L. Lew. "Cytoplasmic calcium buffers in intact human red cells." Journal of Physiology 500, no. 1 (1997): 139–54. http://dx.doi.org/10.1113/jphysiol.1997.sp022005.

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19

Kaźmierczak, Bogdan, and Zbigniew Peradzyński. "Calcium waves with fast buffers and mechanical effects." Journal of Mathematical Biology 62, no. 1 (2010): 1–38. http://dx.doi.org/10.1007/s00285-009-0323-2.

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20

Swann, K., and M. Whitaker. "The part played by inositol trisphosphate and calcium in the propagation of the fertilization wave in sea urchin eggs." Journal of Cell Biology 103, no. 6 (1986): 2333–42. http://dx.doi.org/10.1083/jcb.103.6.2333.

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Sea urchin egg activation at fertilization is progressive, beginning at the point of sperm entry and moving across the egg with a velocity of 5 microns/s. This activation wave (Kacser, H., 1955, J. Exp. Biol., 32:451-467) has been suggested to be the result of a progressive release of calcium from a store within the egg cytoplasm (Jaffe, L. F., 1983, Dev. Biol., 99:265-276). The progressive release of calcium may be due to the production of inositol trisphosphate (InsP3), a second messenger. We show here that a wave of calcium release crosses the Lytechinus pictus egg; the peak of the wave tra

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21

KAZMIERCZAK, BOGDAN, and VITALY VOLPERT. "TRAVELLING CALCIUM WAVES IN SYSTEMS WITH NON-DIFFUSING BUFFERS." Mathematical Models and Methods in Applied Sciences 18, no. 06 (2008): 883–912. http://dx.doi.org/10.1142/s0218202508002899.

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The existence and structural stability of travelling waves of systems of the free cytosolic calcium concentration in the presence of immobile buffers are studied. The proof is carried out by passing to zero with the diffusion coefficients of buffers. Thus, its method is different from Ref. 13 where the existence is proved straightforwardly.

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22

Kotwani, Mansha, and Neeru Adlakha. "Modeling of endoplasmic reticulum and plasma membrane Ca2+ uptake and release fluxes with excess buffer approximation (EBA) in fibroblast cell." International Journal of Computational Materials Science and Engineering 06, no. 01 (2017): 1750004. http://dx.doi.org/10.1142/s204768411750004x.

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Many cells use oscillations in calcium concentration to transmit messages. These oscillations largely depend on the influx of calcium into cytosol from endoplasmic reticulum (ER) and plasma membrane (PM) through various channels and pumps. To analyze the influence of transfer of calcium between different cellular compartments, we develop a mathematical model of calcium dynamics in fibroblast cell. The model involves three important physiological parameters [Formula: see text], and excess buffer approximation (EBA). Finite difference method has been employed to obtain the solution. A computer p

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23

Nowycky, M. C., and M. J. Pinter. "Time courses of calcium and calcium-bound buffers following calcium influx in a model cell." Biophysical Journal 64, no. 1 (1993): 77–91. http://dx.doi.org/10.1016/s0006-3495(93)81342-0.

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24

PANDAY, SUNIL, and KAMAL RAJ PARDASANI. "FINITE ELEMENT MODEL TO STUDY THE MECHANICS OF CALCIUM REGULATION IN OOCYTE." Journal of Mechanics in Medicine and Biology 14, no. 02 (2014): 1450022. http://dx.doi.org/10.1142/s0219519414500225.

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At various stages of fertilization specific spatial and temporal patterns of Ca 2+ are required for oocyte maturation. It is crucial to understand the mechanics of Ca 2+ regulation in cytosol of oocytes, in order to have better understanding of fertilization process. In this paper, a finite element model of cytosolic calcium regulation in oocyte has been developed for a two-dimensional unsteady state case. The model incorporates the important biophysical processes like diffusion, reaction, leak from endoplasmic recticulum (ER), efflux from cytosol to ER via sarco-ER calcium adenosine triphosph

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25

Köhr, G., and I. Mody. "Endogenous intracellular calcium buffering and the activation/inactivation of HVA calcium currents in rat dentate gyrus granule cells." Journal of General Physiology 98, no. 5 (1991): 941–67. http://dx.doi.org/10.1085/jgp.98.5.941.

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Granule cells acutely dissociated from the dentate gyrus of adult rat brains displayed a single class of high-threshold, voltage-activated (HVA) Ca2+ channels. The kinetics of whole-cell Ca2+ currents recorded with pipette solutions containing an intracellular ATP regenerating system but devoid of exogenous Ca2+ buffers, were fit best by Hodgkin-Huxley kinetics (m2h), and were indistinguishable from those recorded with the nystatin perforated patch method. In the absence of exogenous Ca2+ buffers, inactivation of HVA Ca2+ channels was a predominantly Ca(2+)-dependent process. The contribution

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26

Solovey, Guillermo, and Silvina Ponce Dawson. "Observable effects of Ca 2+ buffers on local Ca 2+ signals." Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 368, no. 1933 (2010): 5597–603. http://dx.doi.org/10.1098/rsta.2010.0273.

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Calcium signals participate in a large variety of physiological processes. In many instances, they involve calcium entry through inositol 1,4,5-trisphosphate (IP 3 ) receptors (IP 3 Rs), which are usually organized in clusters. Recent high-resolution optical experiments by Smith & Parker have provided new information on Ca 2+ release from clustered IP 3 Rs. In the present paper, we use the model recently introduced by Solovey & Ponce Dawson to determine how the distribution of the number of IP 3 Rs that become open during a localized release event may change by the presence of Ca 2+ bu

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Lai, Yi Ming, Stephen Coombes, and Rüdiger Thul. "Calcium buffers and L-type calcium channels as modulators of cardiac subcellular alternans." Communications in Nonlinear Science and Numerical Simulation 85 (June 2020): 105181. http://dx.doi.org/10.1016/j.cnsns.2020.105181.

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28

RÅnby, Mats, Tony Gojceta, Kerstin Gustafsson, Kenny M. Hansson, and Tomas L. Lindahl. "Isocitrate as Calcium Ion Activity Buffer in Coagulation Assays." Clinical Chemistry 45, no. 8 (1999): 1176–80. http://dx.doi.org/10.1093/clinchem/45.8.1176.

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Abstract Background: Ca2+ activity close to the physiological concentration of 1.3 mmol/L is essential in blood coagulation. Is this also true for the performance of global diagnostic coagulation assays? We searched for compounds that would buffer Ca2+ activity at ∼1.3 mmol/L without disturbing coagulation reactions and investigated whether such Ca2+ buffering improves diagnostic efficacy in global diagnostic coagulation tests. Methods: Buffering was investigated by mixing CaCl2 and 11 candidate compounds and determining Ca2+ activity. The best candidates were added to mixtures of plasma and t

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Winslow, J. L., S. N. Duffy, and M. P. Charlton. "Homosynaptic facilitation of transmitter release in crayfish is not affected by mobile calcium chelators: implications for the residual ionized calcium hypothesis from electrophysiological and computational analyses." Journal of Neurophysiology 72, no. 4 (1994): 1769–93. http://dx.doi.org/10.1152/jn.1994.72.4.1769.

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1. Evoked neurotransmitter release at the crayfish neuromuscular junction was measured in the presence of the cell-permeant calcium chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-acetotoxymethyl (BAPTA-AM). Excitatory post-synaptic potentials were greatly diminished after application of the intracellular chelator, an effect resulting from attenuation of the rise in the concentration of cytoplasmic Ca2+ ([Ca]i) that is necessary for neurotransmission. However, short-term homosynaptic facilitation of release, the magnitude and time course of which is thought to depend on the a

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30

OHASHI, H., N. UMEDA, N. HIRAZAWA, Y. OZAKI, C. MIURA, and T. MIURA. "Antiparasitic effect of calcium and magnesium ion-free buffer treatments against a common monogeneanNeobenedenia girellae." Parasitology 134, no. 2 (2006): 229–36. http://dx.doi.org/10.1017/s0031182006001430.

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This study investigated a new effective method for controlling the capsalid monogeneanNeobenedenia girellae. We examinedin vitroandin vivothe effect on the percentage survival ofN. girellaein buffers containing different metallic ions. Decreased survival was observed in buffer solutions lacking two ions. In particular, the percentage survival ofN. girellaewas significantly decreased after 10 min exposure to buffer containing neither Ca2+nor Mg2+. Transmission electron microscopic observations showed that treatment with this buffer disrupted intercellular junctions. This significant effect on p

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31

Koepke, John A. "More on Calcium Ion Activity Buffers for Coagulation Testing." Clinical Chemistry 45, no. 9 (1999): 1575–76. http://dx.doi.org/10.1093/clinchem/45.9.1575.

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32

Pethig, R., M. Kuhn, R. Payne, E. Adler, T. H. Chen, and L. F. Jaffe. "On the dissociation constants of BAPTA-type calcium buffers." Cell Calcium 10, no. 7 (1989): 491–98. http://dx.doi.org/10.1016/0143-4160(89)90026-2.

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McCarthy, S. T., J. P. Younger, and W. G. Owen. "Dynamic, spatially nonuniform calcium regulation in frog rods exposed to light." Journal of Neurophysiology 76, no. 3 (1996): 1991–2004. http://dx.doi.org/10.1152/jn.1996.76.3.1991.

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1. In intact rods of the bullfrog, Rana Catesbeiana, that were loaded with Fura-2 by incubation, we made high-resolution measurements of Na:Ca,K exchange currents and measured cytosolic free calcium concentrations during exposure to steps of illumination. The calcium dynamics we observed are indicative of unmanipulated rods because Fura-2 had little effect on calcium buffering within the outer segment. 2. In the dark, the total concentration of calcium within the outer segment, determined by integrating the exchange current, was near 50 microM. The free calcium concentration in darkness was 20

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Jha, Amrita, and Neeru Adlakha. "Finite element model to study the effect of exogenous buffer on calcium dynamics in dendritic spines." International Journal of Modeling, Simulation, and Scientific Computing 05, no. 02 (2014): 1350027. http://dx.doi.org/10.1142/s179396231350027x.

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Dendritic spine plays an important role in calcium regulation in a neuron cell. It serves as a storage site for synaptic strength and receives input from a single synapse of axon. In order to understand the calcium dynamics in a neuron cell, it is crucial to understand the calcium dynamics in dendritic spines. In this paper, an attempt has been made to study the calcium dynamics due to the exogenous buffers, in dendritic spines with the help of a sectional model. The compartments of dendritic spines are discretized using triangular elements. Appropriate boundary conditions have been framed. Fi

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35

Chen, Yinbo, and Victor Matveev. "Pade Approximation of Single-Channel Calcium Nanodomains in the Presence of Cooperative Calcium Buffers." Biophysical Journal 116, no. 3 (2019): 239a—240a. http://dx.doi.org/10.1016/j.bpj.2018.11.1314.

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Jongejan, R. C., J. C. De Jongste, R. C. Raatgeep, I. L. Bonta, and K. F. Kerrebijn. "Effects of changes in osmolarity on isolated human airways." Journal of Applied Physiology 68, no. 4 (1990): 1568–75. http://dx.doi.org/10.1152/jappl.1990.68.4.1568.

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The effects of hypo- and hyperosmolarity on the function of isolated human airways were studied. Changes in osmolarity induced an increasing bronchoconstriction that was proportional to the magnitude of the change in osmolarity. Hypertonicity-induced airway narrowing resulted when buffer was made hypertonic with sodium chloride or mannitol but not with urea. The airways showed no tachyphylaxis to repetitive exposure to hypo- and hypertonic buffer of 200 and 600 mosM, respectively. The bronchoconstriction was not secondary to stimulation of H1 or leukotriene C4/D4 receptors or the release of pr

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Pathak, Kunal B., and Neeru Adlakha. "Finite Element Model to Study One Dimensional Calcium Dyanmics in Cardiac Myocytes." Journal of Multiscale Modelling 06, no. 02 (2015): 1550003. http://dx.doi.org/10.1142/s1756973715500031.

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The multi physical process involving calcium ions regulate expansion and contraction of cardiac myocytes. This mechanism of expansion and contraction of cardiac myocytes is responsible for contraction and expansion of heart for pumping of blood into arteries and receiving blood into heart from vein. Thus calcium dynamics in cardiac myocytes is responsible for the activities of the myocytes cells and functioning of the heart. The specific spatiotemporal calcium ion dynamics is required to trigger, sustain and terminate activity of the cell. In this paper an attempt has been done to propose a mo

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Zhou, Z., and E. Neher. "Mobile and immobile calcium buffers in bovine adrenal chromaffin cells." Journal of Physiology 469, no. 1 (1993): 245–73. http://dx.doi.org/10.1113/jphysiol.1993.sp019813.

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Naik, Parvaiz Ahmad, and Kamal Raj Pardasani. "Three-Dimensional Finite Element Model to Study Effect of RyR Calcium Channel, ER Leak and SERCA Pump on Calcium Distribution in Oocyte Cell." International Journal of Computational Methods 16, no. 01 (2018): 1850091. http://dx.doi.org/10.1142/s0219876218500913.

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Calcium ions control many cellular processes by relaying signals in the form of their spatio-temporal distribution. Dynamics and patterns of calcium concentration such as repetitive waves, coherent oscillations or spatially localized elevations activate diverse physiological functions. Calcium is the most universal second messenger in cells and plays an important role in initiation, sustenance and termination of various activities in cells required for maintaining the structure and function of the cell. Calcium signal at fertilization is necessary for egg activation and exhibits specialized sp

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40

Roberts, S. K., I. Gillot, and C. Brownlee. "Cytoplasmic calcium and Fucus egg activation." Development 120, no. 1 (1994): 155–63. http://dx.doi.org/10.1242/dev.120.1.155.

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Eggs of the marine brown alga, Fucus serratus, exhibit small transient elevations of cytosolic Ca2+ of variable magnitude, corresponding to the onset of the fertilization potential. Microinjection of Ca2+ buffers (BAPTA (1-2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid)) at concentrations sufficient to block any global fertilization-associated Ca2+cyt elevation did not inhibit egg activation (monitored as exocytosis of cell wall) or subsequent development. However, egg activation could be inhibited with higher buffer concentrations. Br2BAPTA (Kd = 1.6 micromolar) was a more effective i

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41

Rintoul, Gordon L., and Kenneth G. Baimbridge. "Effects of calcium buffers and calbindin-D28k upon histamine-induced calcium oscillations and calcium waves in HeLa cells." Cell Calcium 34, no. 2 (2003): 131–44. http://dx.doi.org/10.1016/s0143-4160(03)00041-1.

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42

Miller, A. L., R. A. Fluck, J. A. McLaughlin, and L. F. Jaffe. "Calcium buffer injections inhibit cytokinesis in Xenopus eggs." Journal of Cell Science 106, no. 2 (1993): 523–34. http://dx.doi.org/10.1242/jcs.106.2.523.

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A slow cortical wave of high calcium accompanies the elongation of cleavage furrows in medaka fish eggs as well as in Xenopus eggs. We explored the role of such waves by injecting calcium buffers into Xenopus eggs at various times before and during first and second cleavage. Injection earlier than about 15 minutes before first cleavage normally starts delays it for hours. Injection between about 15 minutes and a few minutes before cleavage normally starts allows a (short) furrow to form on time but usually yields an eccentric one. This forms away from the injection side, often as far off-cente

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Díaz, M. E., A. W. Trafford, and D. A. Eisner. "The Effects of Exogenous Calcium Buffers on the Systolic Calcium Transient in Rat Ventricular Myocytes." Biophysical Journal 80, no. 4 (2001): 1915–25. http://dx.doi.org/10.1016/s0006-3495(01)76161-9.

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44

Matveev, Victor, Richard Bertram, and Arthur Sherman. "Computational Study Of The Effect Of Calcium Buffers On The Calcium Current Cooperativity Of Exocytosis." Biophysical Journal 96, no. 3 (2009): 659a—660a. http://dx.doi.org/10.1016/j.bpj.2008.12.3484.

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J�rgens, M., L. H. Hepler, B. A. Rivers, and P. K. Hepler. "BAPTA-calcium buffers modulate cell plate formation in stamen hairs ofTradescantia: evidence for calcium gradients." Protoplasma 183, no. 1-4 (1994): 86–99. http://dx.doi.org/10.1007/bf01276816.

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Naik, Parvaiz Ahmad, and Kamal Raj Pardasani. "Two Dimensional Finite Element Model to Study Calcium Distribution in Oocytes." Journal of Multiscale Modelling 06, no. 01 (2015): 1450002. http://dx.doi.org/10.1142/s1756973714500024.

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Cytosolic free calcium concentration is a key regulatory factor and perhaps the most widely used means of controlling cellular function. Calcium can enter cells through different pathways which are activated by specific stimuli including membrane depolarization, chemical signals and calcium depletion of intracellular stores. One of the important components of oocyte maturation is differentiation of the Ca 2+ signaling machinery which is essential for egg activation after fertilization. Eggs acquire the ability to produce the fertilization-specific calcium signal during oocyte maturation. The c

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Newman, George C., Frank E. Hospod, Clifford S. Patlak, et al. "Calcium Compartments in Brain." Journal of Cerebral Blood Flow & Metabolism 22, no. 4 (2002): 479–89. http://dx.doi.org/10.1097/00004647-200204000-00012.

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Excellent progress has been made toward understanding the physiology and pharmacology of specific calcium-related cellular processes of the brain, but few studies have provided an integrated view of brain calcium kinetics. To further the knowledge of the size and binding properties of brain calcium compartments, the authors have conducted a series of experiments in hippocampal brain slices exposed to high and low extracellular calcium. Slices were incubated in buffers containing 0.001 to 4.5 mmol/L calcium for up to 75 minutes. Slice calcium content was analyzed by three methods: exchange equi

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Hacker, Kyle, and Kathryn F. Medler. "Mitochondrial Calcium Buffering Contributes to the Maintenance of Basal Calcium Levels in Mouse Taste Cells." Journal of Neurophysiology 100, no. 4 (2008): 2177–91. http://dx.doi.org/10.1152/jn.90534.2008.

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Taste stimuli are detected by taste receptor cells present in the oral cavity using diverse signaling pathways. Some taste stimuli are detected by G protein–coupled receptors (GPCRs) that cause calcium release from intracellular stores, whereas other stimuli depolarize taste cells to cause calcium influx through voltage-gated calcium channels (VGCCs). Although taste cells use two distinct mechanisms to transmit taste signals, increases in cytosolic calcium are critical for normal responses in both pathways. This creates a need to tightly control intracellular calcium levels in all transducing

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Hall, J. D., S. Betarbet, and F. Jaramillo. "Endogenous buffers limit the spread of free calcium in hair cells." Biophysical Journal 73, no. 3 (1997): 1243–52. http://dx.doi.org/10.1016/s0006-3495(97)78157-8.

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Lamb, T. D., H. R. Matthews, and V. Torre. "Incorporation of calcium buffers into salamander retinal rods: a rejection of the calcium hypothesis of phototransduction." Journal of Physiology 372, no. 1 (1986): 315–49. http://dx.doi.org/10.1113/jphysiol.1986.sp016011.

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