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Journal articles on the topic 'Callus induction'

Author: Grafiati

Published: 4 June 2021

Last updated: 26 July 2025

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1

Yang, Yali, Peiting He, Jiayi Zhang, et al. "Induction of callus in Cassia mimosoides." E3S Web of Conferences 189 (2020): 02017. http://dx.doi.org/10.1051/e3sconf/202018902017.

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In this study, the young leaves of Cassia mimosoides were used as explants. On the basis of 1/2 MS +2.0 mg/L 6-BA +0.5 mg/L NAA medium, we observed the effect of adding different factors on the callus induction of C. mimosoides. The results shown that after 20 days of callus induction, it was found that the addition of 150 mg/L vitamin C (VC) did not affect the induction efficiency, but the growth of callus became slower; and when the concentration was higher than 150 mg/L, the formation of callus would be inhibited. The induction of callus would be restrained while adding different concentrat

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Jamil, Shakra, Rahil Shahzad, Ghulam Mohyuddin Talha, et al. "Optimization of Protocols for In Vitro Regeneration of Sugarcane (Saccharum officinarum)." International Journal of Agronomy 2017 (2017): 1–8. http://dx.doi.org/10.1155/2017/2089381.

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Sugarcane contributes 60–70% of annual sugar production in the world. Somaclonal variation has potential to enhance genetic variation present within a species. Present study was done to optimize an in vitro propagation protocol for sugarcane. The experiments included four varieties, 9 callus induction media, 27 regeneration media, and 9 root induction media under two-factor factorial CRD. Data were recorded on callus induction, embryogenic callus formation, shoot elongation (cm), root induction, and plant regeneration. Statistically significant differences existed between genotypes and treatme

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3

Wang, Y. "Callus induction and frond regeneration in Spirodela polyrhiza." Czech Journal of Genetics and Plant Breeding 52, No. 3 (2016): 114–19. http://dx.doi.org/10.17221/134/2015-cjgpb.

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4

Haris, Muhammad, Sumbal Rasheed, Shahroz Rahman, Ishmal Fatima, Ayesha Mazhar, and Farrukh Azeem. "Tissue Culture Optimization: Maize Callus Induction and Factors Affecting the Callus Efficiency." Integrative Plant Biotechnology 3, no. 2 (2025): 73–99. https://doi.org/10.55627/pbiotech.003.02.1288.

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Maize (Zea mays L.), also known as the queen of cereals, is one of the most important food crops in terms of food security, the world economy, and global agriculture. The high yield, diverse climate adaptability, and extensive applications of maize in food, feed, and industrial products make it a highly demanding crop. The traditional methods of maize propagation are slow and often result in less yield with undesired traits due to large-scale variations. However, the advancements in agricultural biotechnology, particularly in plant tissue culture, have made it possible to propagate maize cultu

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Coll, Y., A. Gonzalez, J. Alfonso, R. Armas, and M. Pujol. "Callus induction from indica rice coleoptiles." International Rice Research Notes 22, no. 2 (1997): 17. https://doi.org/10.5281/zenodo.7008385.

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This article 'Callus induction from indica rice coleoptiles' appeared in the International Rice Research Notes series, created by the International Rice Research Institute (IRRI) to expedite communication among scientists concerned with the development of improved technology for rice and rice-based systems. The series is a mechanism to help scientists keep each other informed of current rice research findings. The concise scientific notes are meant to encourage rice scientists to communicate with one another to obtain details on the research reported.

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Rajbhandari, Nirmala, and Anne-Marie Stomp. "Embryogenic Callus Induction in Fraser Fir." HortScience 32, no. 4 (1997): 737–38. http://dx.doi.org/10.21273/hortsci.32.4.737.

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Mass propagation of Fraser fir [Abies fraseri (Pursh) Poir], a valuable Christmas tree species in the United States, is problematic because methods currently used are inadequate. According to our results with somatic embryogenesis, the culturing methods used with other Abies species are applicable to Fraser fir. Stage 1 somatic embryogenic callus, characterized by suspensor cells and embryo heads, was obtained at low frequency using Schenk and Hildebrandt medium supplemented with 5 mm glutamine, 0.05% casein hydrolysate, 0.01% myoinositol, 2% sucrose, 5 μM benzyladenine, and 0.6% agar. The dev

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Yelnititis, Yelnititis. "INDUKSI KALUS EMBRIOGENIK DAN EMBRIO SOMATIK DARI EKSPLAN DAUN KULIM (Scorodacarpus borneensis Becc.)." Jurnal Pemuliaan Tanaman Hutan 14, no. 2 (2020): 73–81. http://dx.doi.org/10.20886/jpth.2020.14.2.73-81.

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Kulim is one of woody plant that have multifunction as wood source and for spice and medicinal. Generative propagation of this plant have trouble because seed use limited. The use of leaf segment through somatic embryogenesis to solve the problem. The objective of this study is to obtain the best treatment to embryogenic callus induction. The modification of basal medium of Murashige and Skoog was used as growth medium. The experiment was conducted in three stages are callus induction, embryogenic callus and somatic embryo induction. The treatment of 2,4-D (3,0 – 12 mg/l) used for callus induc

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Li, Chunjia, Xujuan Li, Xiuqin Lin, et al. "Genotypic variation in the response to embryogenic callus induction and regeneration in Saccharum spontaneum." Plant Genetic Resources: Characterization and Utilization 19, no. 2 (2021): 153–58. http://dx.doi.org/10.1017/s1479262121000198.

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AbstractEmbryogenic callus induction and regeneration are useful in many aspects of plant biotechnology, especially in the functional characterization of economically important genes. However, in sugarcane, callus induction and regeneration vary across genotypes. Saccharum spontaneum is an important wild germplasm that confers disease resistance and stress tolerance to modern sugarcane cultivars, and its genome has been completely sequenced. The aim of this study was to investigate the effect of genetic variations on embryogenic callus induction and regeneration in S. spontaneum and to screen

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9

WIJAYA, BARAELA EZRA, RINDANG DWIYANI, and NI NYOMAN ARI MAYADEWI. "Induksi dan Multiplikasi Kalus Eucalyptus sp. pada Berbagai Media Callus Induction Medium (CIM) Secara In Vitro." Agrotrop : Journal on Agriculture Science 13, no. 1 (2023): 76. http://dx.doi.org/10.24843/ajoas.2023.v13.i01.p07.

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Callus Induction and Multiplication of Eucalyptus sp. on Various Media Callus Induction Medium (CIM) In Vitro. Callus induction in vitro can be done by planting specific part of plant such as leaf, root, stem, etc in aseptic condition by using medium that contains 2,4-D, a plat regulator for promoting callus formation. One of the objectives of callus production in vitro is to form secondary metabolites, because eucalyptus is known as a producer of essential oils. This research is an initial study for in vitro production of secondary metabolites for further research. The aim of this study was t

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N'GUESSAN, Affoué Sylvie Rachelle, Koutouan AYOLIE, Sopie Edwige Salomé YAPO, et al. "The Induction of High-Frequency Callus with an Elite Cotton (Gossypium Hirsutum L.) from Côte d'Ivoire." JOURNAL OF ADVANCES IN BIOTECHNOLOGY 8 (November 7, 2019): 1104–17. http://dx.doi.org/10.24297/jbt.v8i0.8503.

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In cotton, factors such as carbon sources, hormonal combination, and type of explant influence the responses to callus induction. In this study callus induction was optimized to get high-frequency callus in cotton. Different carbon sources were tested, and among them, glucose at 3% was found to be the best carbohydrate in callus induction. Culture medium supplemented with 2,4-D (0.1 mg.L-1) and KIN (0.5 mg.L-1) gave a high callogenesis response, unlike other hormonal combinations. With explants, hypocotyl (72.93%) appeared easier to induce callus than cotyledon (48.75%) following root (37.5%).

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Royani, J. I., H. Khairiyah, F. R. Mira, Karyanti, and N. Watanabe. "The effect of callus induction media for somatic embryo formation in Hevea brasiliensis Muell. Arg." IOP Conference Series: Earth and Environmental Science 1160, no. 1 (2023): 012007. http://dx.doi.org/10.1088/1755-1315/1160/1/012007.

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Abstract The method for micro-propagation of rubber (Hevea brasiliensis Muell. Arg) by somatic embryo has been conducted. The aim of the research will describe the effect of media used for callus induction on the somatic embryo formation of rubber plants. Embryonic callus had been induction in 3 media, i.e.; MH1, MH2, and MH3. After callus induction, all callus were induced in media for embryo somatic expression. Maturation of cotyledons was performed using liquid media with filter paper as a bridge to absorb the media. The results showed that the percentage of callus was induced differently f

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12

Kim, Jaewook, Jung-Woo Lee, and Ick-Hyun Jo. "Multiple Transcriptomic Networks Regulate the Callus Development Process in Panax ginseng." Agronomy 15, no. 5 (2025): 1244. https://doi.org/10.3390/agronomy15051244.

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Callus induction is one of the most important techniques in plant-based industries. Important features in the use of callus induction are the maintenance of pluripotency and the proliferation of cells. Although the importance of callus induction is also understood in ginseng, there are no studies on the genetic modules associated with callus induction and growth regulation. Panax ginseng embryo tissue was wounded and cultured in callus-inducing media, and its time-course physiology was observed. Time-course callus samples were collected for total RNA extraction and RNA-Seq analysis using the I

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Özgen, Murat, Melahat A. Birsin, and Berk Benlioglu. "Biotechnological characterization of a diverse set of wheat progenitors (Aegilops sp. and Triticum sp.) using callus culture parameters." Plant Genetic Resources 15, no. 1 (2015): 45–50. http://dx.doi.org/10.1017/s1479262115000350.

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It is known that genetic diversity is the most important factor in classical and modern plant breeding. The considerable increase in the number of transgenic crops reveals the value of new plant genetic resources. In this study, a set of 12 wheat progenitors were screened for tissue culture parameters such as callus induction, callus weight, regeneration capacity of callus and callus efficiency using mature embryos. Embryos were excised from imbibed seeds of the progenitors. The excised embryos were placed scutellum upwards in dishes containing 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for

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14

Mahood, Huda E. "Effect of Plant Growth Regulators and Explant Source on the Induction of Callus of Dianthus caryophyllus L." Basrah Journal of Agricultural Sciences 34, no. 2 (2021): 100–106. http://dx.doi.org/10.37077/25200860.2021.34.2.08.

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In order to investigate the possibility for in in vitro fast regeneration of Dianthus caryophyllus, different concentrations of Kinetin (Kin), 2,4-Dichlorophenoxy Acetic Acid (2,4-D) had been tested for induction of callus by leaves and stems explant using Murashige and Skoog (MS) medium. After two months in culture, response callus in the induction rate, fresh and dry weight, texture and color were evaluated. MS medium that contained 4.5 mg.L-1 2, 4-D was suitable for callus induction with leaf explants. The combination of 0.4 mg.L-1 Kin and 2.0 mg.L-1 2, 4-D also demonstrated a wonderful ind

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15

Fuke, Pramod, Sangeeta Ahuja, and Narayan B Pandhure. "In Vitro Callus Induction studies in Salvadora persica L." International Journal of Science and Research (IJSR) 10, no. 3 (2021): 976–78. https://doi.org/10.21275/sr21312194946.

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16

Monteiro, Marcília Gabriella Tavares, Joanne Moraes de Melo Souza, Hugo Alves Pinheiro, et al. "In Vitro Induction of Callus and Flowers in Immature Oil Palm Inflorescences." Journal of Agricultural Studies 8, no. 4 (2020): 712. http://dx.doi.org/10.5296/jas.v8i4.16114.

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This work aimed to investigate the effects of different combinations of auxin (0, 225 and 450 µM Picloram) and Murashige and Skoog salts (50% and 100% MS salt concentrations, respectively named as ½ MS and MS) in mediate the induction of callus and flowers from immature inflorescences (developmental stages I-9, I-12 and I-15) of the oil palm BRS – Manicoré hybrid. In I-9 inflorescence explants, the rate of callus induction was 17% higher in ½ MS than in MS; while in both I-12 and I-15 inflorescence explants, callus induction was over than 95% regardless of the MS salt concentration. The higher

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17

Guo, Guimei, Shisen Liu, Shuwei Zhang, et al. "Generic Workflow of a Highly Effective and Easy Anther Culture Method for Both Japonica and Indica Rice." Plants 13, no. 17 (2024): 2531. http://dx.doi.org/10.3390/plants13172531.

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As one of the most important staple crops in the world, rice plays a pivotal role in world food security. The creation of doubled haploids based on anther culture is an important technology for rice breeding. However, at present, rice anther culture technology still faces many problems, such as genotype dependency, especially genotypes of indica rice. In this study, fifteen rice genotypes, including twelve japonica rice genotypes and three indica rice genotypes, were randomly selected and used to study anther culture by using a modified M8 medium. The results showed that the total callus induc

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18

Nie, Shuai, Yue Wang, Yong Yan, et al. "Effects of a New Plant Growth Regulator on Callus Induction from Immature Embryo Explants of Korean Pine (Pinus koraiensis)." Forests 14, no. 12 (2023): 2413. http://dx.doi.org/10.3390/f14122413.

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Somatic embryogenesis is currently the most promising technique for the large-scale production and breeding of conifer species. Nonetheless, the low explant induction rate in this process hampers the development of improved materials. In this study, three immature Korean pine (Pinus koraiensis) embryos capable of induction were used as experimental materials. Various concentrations of brassinolide (BL), melatonin (Mel), salicylic acid (SA), glutathione (GSH), and ascorbic acid (AsA) were added to induce embryogenic callus tissue. The results show that BL had the most significant positive effec

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杨, 通银. "Induction Research on Vaccinium ashei Callus." Botanical Research 11, no. 01 (2022): 22–28. http://dx.doi.org/10.12677/br.2022.111004.

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20

Checheneva, T. N., L. V. Luchko, and M. O. Dykun. "Callus induction in mature corn embryos." Biopolymers and Cell 15, no. 3 (1999): 237–40. http://dx.doi.org/10.7124/bc.00051f.

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21

GONZALEZ-BENITO, M. ELENA, and P. G. ALDERSON. "Callus Induction and Plant Regeneration inAlstroemeria." Journal of Experimental Botany 43, no. 2 (1992): 205–11. http://dx.doi.org/10.1093/jxb/43.2.205.

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22

Malini, N., C. R. Anandakumar, R. Gnanam, and S. Hari Ramakrishnan. "Effect of harmones on callus induction in Maize (Zea mays L.)." Journal of Applied and Natural Science 10, no. 1 (2018): 202–9. http://dx.doi.org/10.31018/jans.v10i1.1605.

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Callus induction from explants is a critical process in regeneration, micropropagation and transformation of maize (Zea mays L.) plants. Formation of callus from plant tissues on culture is affected by several factors. This study revealed to establish the effect of genotype, source of explants and auxin concentration on callus induction from five genotypes UMI 757 (G1), UMI 615 (G2), UMI 112 (G3), UMI 285 (G4) and CO 1 (G5) and one hybrid CO H (M) 5 (G6). Callus induction of the six maize varieties was investigated using immature embryos (E1), leaf bits (E2), root tips (E3), hypocotyls (E4) an

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Khan, Md Niuz Morshed, Md Monirul Islam, and Dr Md Shahidul Islam. "Studies on In Vitro Response to Callus Induction and Regeneration of Five High Yielding Indica rice Varieties." Journal of Horticulture and Plant Research 7 (August 2019): 97–104. http://dx.doi.org/10.18052/www.scipress.com/jhpr.7.97.

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Due to growing population, there is an increasing demand of rice production but the productivity of rice is lessened day by day. To overcome this problem various biotechnological tools can be used for developing various rice varieties. However, the lack of a simple and efficient protocol for callus induction, embryogenic callus formation and quick plant regeneration in this cereal crop. In this study embryogenic calli from mature seeds of five indica rice varieties viz. Binadhan-5, Binadhan-6, BRRI dhan-48, BRRI dhan-58 and IR-64 were observed that is done in four different types of media comp

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Mamun, AA, MM Islam, NEK Alam, and S. Noushin. "In vitro Callus Induction under NaCl Salt Stress and Subsequent Plant Regeneration in Oats (Avena sativa L.)." Plant Tissue Culture and Biotechnology 30, no. 2 (2020): 253–66. http://dx.doi.org/10.3329/ptcb.v30i2.50695.

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An effort was made to identify the in vitro responsiveness for callus induction in NaCl (salt stress) and subsequent plant regeneration of oats (Avena sativa L.). Callus induction was tested in different concentrations (0, 5, 10, 15 and 20 mg/l) of NaCl salt added in MS. The experiment was laid out in CRD considering 2,4-D growth regulator and NaCl salt as factors. The best response in callus induction was observed on MS containing 3 mg/l 2,4- D and 5 mg/l NaCl where the frequency of callus induction was 68.57%, callus weight was 0.36 g and callus diameter was 7.63 mm. During regeneration, the

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Al-Hamidi, A. O. A., S. H. A. Al-Hadedy, and A. Z. A. K. Bashi. "Effect of 2,4-D and NAA in Callus Induction and Differentiation from Different Explants of Moringa Oleifera Lam." IOP Conference Series: Earth and Environmental Science 1252, no. 1 (2023): 012091. http://dx.doi.org/10.1088/1755-1315/1252/1/012091.

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Abstract This study was carry out in the Laboratory of Plant Cells and Tissue Culture / Department of Forestry Sciences in the College of Agriculture and Forestry / University of Mosul during the year 2021-2022 to study the effect of adding different concentrations of 2,4-Dichloro-Phenoxyacetic acid (2,4-D) or Naphthalene Acetic Acid (NAA) to the MS medium in the induction of callus from different parts of the Moringa oleifera Lam. and then study the effect of different concentrations of 6-Benzyladenine (BA) and Kinetin (Kin) on the differentiation of callus into buds and embryogenesis. Concer

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Mo, Vu Thi, Tran Van Huynh, Le Trong Nghia, Hoang Thanh Tung, Nguyen Ngoc Lam, and Duong Tan Nhut. "CALLUS INDUCTION FROM BRANCHES OF KAPPAPHYCUS STRIATUS UNDER DIFFERENT CULTURE CONDITIONS." Vietnam Journal of Biotechnology 16, no. 2 (2018): 301–9. http://dx.doi.org/10.15625/1811-4989/16/2/13441.

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Kappaphycus striatus is growing in some central coastal provinces as a source of carrageenan extract. It is mainly propagated in the form of vegetative reproduction and sporulation. However, this method still has some limitations. At present, there is no report on the micropropagation of this species through callus induction. In this study, effect of plant growth regulators (PGRs) (Naphthalene acetic acid [NAA] and 6-bezyl amino purine [BAP]), intensity of light and concentration of agar on callus induction derived from branches of Kappaphycus striatus maintaned in laboratory for 1 month were

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Adju, Fitria Y. Hs, Jusna Ahmad, Devi Bunga Pagalla, Novri Youla Kandowangko, Indriati Husain, and Lindawaty Isima. "In Vitro Callus Induction from (Antidesma bunius L.) Leaves." BIOVALENTIA: Biological Research Journal 11, no. 1 (2025): 40–46. https://doi.org/10.24233/biov.11.1.2025.451.

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Antidesma bunius L. is a tree species that has ecological and economic benefits, and has many bioactivities. Propagation of this tree can be done in several ways, one of which is callus induction. Callus is a collection of undifferentiated cells that can develop into new plants with the help of ZPT. The purpose of this study was to determine the effect and optimum combination of ZPT 2,4-D and BAP on the induction of leaf callus of A. bunius L. The research design was a non-factorial Completely Randomised Design (CRD) with 4 treatments namely P0: MS without the addition of zpt; P1: MS + 1 ppm B

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Lu, Ruiju, Zhiwei Chen, Runhong Gao, et al. "Genotypes-Independent Optimization of Nitrogen Supply for Isolated Microspore Cultures in Barley." BioMed Research International 2016 (2016): 1–8. http://dx.doi.org/10.1155/2016/1801646.

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To establish a high-efficiency system of isolated microspore culture for different barley genotypes, we investigated the effects of nitrogen sources and concentrations on callus induction and plant regeneration in different barley genotypes. The results showed that the organic nitrogen sources greatly increased the callus induction, and the great reduction of total nitrogen sources would significantly decrease the callus induction. And the further optimization experiments revealed that the increasing of organic nitrogen sources was much important in callus induction while it seemed different i

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Gao, Lingling, Jingjing Liu, Liao Liao, et al. "Callus Induction and Adventitious Root Regeneration of Cotyledon Explants in Peach Trees." Horticulturae 9, no. 8 (2023): 850. http://dx.doi.org/10.3390/horticulturae9080850.

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Callus induction is a key step in establishing plant regeneration and genetic transformation. In this study, we present a comprehensive large-scale investigation of the callus induction rate (CIR) in peach trees, which revealed significant variability within the peach germplasm. Notably, the late-maturing cultivars exhibited significantly higher levels of CIR. Moreover, cultivars characterized as having high CIR exhibited potential for the development of adventitious roots (ARs) during callus induction, and a positive correlation was observed between CIR and the ability to regenerate ARs. Howe

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Lee, Jin-Hwa, Khawaja Muhammad Imran Bashir, Gabriel Tirtawijaya, Bertoka Fajar Surya Perwira Negara, and Jae-Suk Choi. "Establishment of Effective Callus Induction in the Economically Important Brown Seaweed Ecklonia cava." Applied Sciences 14, no. 8 (2024): 3480. http://dx.doi.org/10.3390/app14083480.

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The edible brown seaweed, Ecklonia cava, is highly valued for its bioactive compounds, and is widely used in food supplements and functional foods. The increasing demand for this seaweed in the food industry emphasizes the necessity for sustainable cultivation practices. This study focused on inducing callus in the meristem and stipe of E. cava using different culture media: Provasoli’s enriched seawater medium (PESI), enriched artificial seawater medium (ESAW), artificial enriched seawater medium (ASP2), or Von Stosch’s enriched seawater medium (VS). Various abiotic stress factors (photoperio

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T. B., Kamble, Sawardekar S. V., Palshetkar M. G., et al. "Optimizing the Protocol for In vitro Regeneration through Callus in Rice Varieties, viz; Ratnagiri-8, Karjat Shatabdi and Karjat-3." Journal of Advances in Biology & Biotechnology 27, no. 11 (2024): 1533–43. https://doi.org/10.9734/jabb/2024/v27i111740.

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The investigation entitled “In vitro regeneration studies in rice (Oryza sativa L.)” was carried out in tissue culture laboratory at Plant Biotechnology Centre, College of Agriculture, Dapoli-Ratnagiri (M.H.) in Completely Randomized Design with 3 replications. The aim of the research was to set up the in vitro regeneration protocol in the three rice varieties, viz, Ratnagiri-8, Karjat Shatabdi, Karjat-3 with an objective to investigate suitable callus induction medium and develop an efficient regeneration technique for genetic transformation. Mature seed embryo was used as explant for callus

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Kumar, Sunil, Virendra Kumar, Manoj Kumar Sharma, et al. "Effects of different plant growth regulators on in vitro callus induction in physic nut (Jatropha curcus L.)." Journal of Applied and Natural Science 7, no. 1 (2015): 30–37. http://dx.doi.org/10.31018/jans.v7i1.559.

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Jatropha (Jatropha curcas L.) is an oil bearing crop growing in tropical and subtropical parts of the world. The present study was undertaken to investigate the effects of different plant growth regulators on in vitro callus induction in physic nut (J. curcus). In the present study, it was observed that all the explants viz., leaf lamina, petioles, nodal segments and cotyledonary nodes showed good callus induction responses on various culture media thus tried. Leaf lamina and petioles showed 100.0% callus induction responses on different MS media supplemented with auxins and cytokinins alone o

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Setiaji, Arkan, RR Rifka Annisa, Rumiyati Rumiyati, and Endang Semiarti. "Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum)." Biosaintifika: Journal of Biology & Biology Education 12, no. 1 (2020): 35–41. http://dx.doi.org/10.15294/biosaintifika.v12i1.21704.

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Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medi

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Priyadarshani K., Anjana, Prashanth B., Prathik K., Sriya Reddy P., and Srinivas Naik K. "IN VITRO CALLUS INDUCTION AND REGENERATION OF ORYZA SATIVA L. VAR. MTU1075 (PUSHYAMI)." International Journal of Advanced Research 12, no. 05 (2024): 676–81. http://dx.doi.org/10.21474/ijar01/18770.

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In vitro inducement of callus and plant regeneration capacity were investigated using mature embryos of the Indian rice (Oryza sativa L.) variety MTU1075. The investigation was conducted using callus induction media (Murashige and Skoog, 1962) with varying concentrations of 2, 4-D, namely 1.0, 1.5, 2.0, 2.5, 3.0, and 3.5mg/l. It shows the maximum degree of callus induction at 2 mg/l 2,4-D. The maximum callus induction rate was 86%. Plant regeneration efficacy of in vitro generated plantlets was studied then successfully transferred into soil conditions.

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yun, R. Q. A’, D. Dinarti, A. Husni, and M. Kosmiatin. "Callus regeneration and polyploidy induction of Allium cepa L var. Bima Brebes using oryzalin." IOP Conference Series: Earth and Environmental Science 948, no. 1 (2021): 012043. http://dx.doi.org/10.1088/1755-1315/948/1/012043.

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Abstract Polyploidy induction could increase shallot bulb-size to raise consumer preference and local shallot productivity. The research aimed to obtain an effective method of polyploidy induction on callus of onion (Allium cepa) var. Bima Brebes. The experiment was consisted of two experimental steps, which were callus induction of onion and polyploid induction of the callus. A 1×1 cm callus was treated by two drops of oryzalin with concentrations 0, 25, 50, 75, 100, and 120 μM. The ploidy level was identified based on morphological trait, stomatal analysis and DNA content using a flow cytome

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Anand, Deep Chhavi, Rishikesh Meena, and Vidya Patni. "IN VITRO CALLUS INDUCTION AND COMPARATIVE GC-MS ANALYSIS OF METHANOLIC EXTRACTS OF CALLUS AND LEAF SAMPLES OF AMPELOCISSUS LATIFOLIA (ROXB.) PLANCH." International Journal of Pharmacy and Pharmaceutical Sciences 10, no. 9 (2018): 68. http://dx.doi.org/10.22159/ijpps.2018v10i9.27879.

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Objective: The aim of the present study was to develop a callus induction protocol and comparative study of therapeutic phytochemicals present in in vivo leaf and in vitro callus extracts through Gas Chromatography-Mass Spectrometry analysis.Methods: Murashige and Skoog media was used as culture media for callus induction. In vitro callus induction protocol was developed by studying the effects of various plant growth regulators like auxin, 2, 4-D (2,4-dichlorophenoxyacetic acid), NAA (naphthalic acetic acid), alone and in combination with cytokinin BAP (benzyl aminopurine), on leaf and stem e

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Kyriienko, A. V., M. F. Parii, Yu V. Symonenko, M. V. Kuchuk, and N. L. Shcherbak. "Callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L." Faktori eksperimental'noi evolucii organizmiv 25 (August 30, 2019): 237–42. http://dx.doi.org/10.7124/feeo.v25.1169.

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Aim. To develop an effective protocol for callus induction from shoot apical meristem in Triticum spelta L. and T. aestivum L. Methods. Plant material: spelt “Europe” and common wheat “Bunchuk”. For this research we used shoot apical meristems from 3-days plants. For callus induction we proposed 4 media with different concentration of 2,4-D, picloram, NAA and AgNO3. Explants were growing in dark during 21 day at + 25 C. Results. Calli were transparent and mild, less than 8 mm. For callus induction positive effect were shown on media with 2,4-D and picloram. At the same time, NAA was not such e

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Purnamaningsih, Ragapadmi. "Induksi Kalus dan Optimasi Regenerasi Empat Varietas Padi melalui Kultur In Vitro." Jurnal AgroBiogen 2, no. 2 (2016): 74. http://dx.doi.org/10.21082/jbio.v2n2.2006.p74-80.

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<p class="p1">A study was conducted at the Tissue Culture Laboratory of ICABIOGRAD, Bogor, to obtain an optimum medium formulation for calli regenerations of for rice varities (Ciherang, Cisadane, IR64, and T-309). The research activities were done in five steps, i.e., callus induction, callus regeneration, shoot multiplication, root formation, and plant acclimatization. The type of explants used in the study was embriozygotic explants. Five media formulations were used for the callus induction, while four media formulations were used for the callus regeneration. The results showed that

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Xavier, Lucas Rodrigues, Caio Cezar Guedes Corrêa, Roberta Pena da Paschoa, et al. "Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction in Carica papaya L." Plants 12, no. 22 (2023): 3891. http://dx.doi.org/10.3390/plants12223891.

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Sex segregation increases the cost of Carica papaya production through seed-based propagation. Therefore, in vitro techniques are an attractive option for clonal propagation, especially of hermaphroditic plants. Here, we performed a temporal analysis of the proteome of C. papaya calli aiming to identify the key players involved in embryogenic callus formation. Mature zygotic embryos used as explants were treated with 20 μM 2,4-dichlorophenoxyacetic acid to induce embryogenic callus. Total proteins were extracted from explants at 0 (zygotic embryo) and after 7, 14, and 21 days of induction. A t

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CAI, Xiao-Dong, Gui-Yuan WANG, and Wen-Juan CAO. "In vitro Induction and Proliferation of Callus from Immature Cotyledons and Embryos of Juglans regia cv. 'Xiangling'." Notulae Botanicae Horti Agrobotanici Cluj-Napoca 41, no. 2 (2013): 378. http://dx.doi.org/10.15835/nbha4129134.

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Callus holds great potential for biotechnology applications in plants. In this study, several experiments were performed to establish a protocol for in vitro induction of good quality callus by using immature cotyledons and embryos of Juglans regia cv. â€˜Xianglingâ€™ as the explants. DKW basal media supplemented with different concentrations of plant growth regulators were applied to determine the induction rate, texture and color of the recovered callus. Results showed that the optimum culture medium for the callus induction from these explants was a DKW basal medium consisting of 1 mg/L BA,

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Santos, Maurício Reginaldo Alves dos. "Callus induction in Bertholletia excelsa immature seeds." Journal of Biotechnology and Biodiversity 4, no. 4 (2013): 283–89. http://dx.doi.org/10.20873/jbb.uft.cemaf.v4n4.santos.

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Bertholletia excelsa H.B.K.,known as Brazil nuttree, belongs to the Lecythidaceae botanic family and is endemic to the AmazonianRegion. Its nuts have high economic and nutritional value. The objective of this work was to establish efficient protocols for callus induction from immature seeds of B.excelsa, as a preliminary step to its further micropropagation. After disinfestation, fragments of immature seeds were cultivated in WPM (Wood Plant Medium) supplemented with 2,4-D (0, 1, 2, 4 and 8 mg.L-1) and TDZ (0, 1.6 and 3.2 mg.L-1) in factorial combination. Twenty-one days later the callus induc

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Tamimi, Samih M., and Halima Othman. "Callus Induction and Regeneration from Germinating Mature Embryos of Wheat (Triticum aestivum L.)." Sains Malaysiana 50, no. 4 (2021): 889–96. http://dx.doi.org/10.17576/jsm-2021-5004-01.

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A high-performance protocol for callus induction was devised using germinating mature embryos of two local wheat (Triticum aestivum L.) landraces as explant. The results showed that callus development from germinating embryos was rapid starting one day after culture with an induction rate 20 to 25% higher than those of soaked embryos. In addition, the mean rate of growth of callus developed from germinating embryos was 60 to 70% higher than those cultured from soaked embryos. This study also demonstrated a higher frequency of green spots formation(48 to 56%)on callus derived from germinating e

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Jaramillo, J., and W. L. Summers. "Tomato Anther Callus Production: Solidifing Agent and Concentration Influence Induction of Callus." Journal of the American Society for Horticultural Science 115, no. 6 (1990): 1047–50. http://dx.doi.org/10.21273/jashs.115.6.1047.

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Anthers from. three tomato cultivars, `L-680A', `Ailsa Craig', and `Licato', were plated on DBM1 medium solidified with one of four solidifying agents, Bacto-agar, Gelrite, Noble agar, or Phytagar, to evaluate their ability to promote initiation and growth of tomato anther callus. The optimum concentration of each solidifying agent was compared with a liquid control. Optimum levels of the various solidifying agents were (in g·liter-1) Phytagar, 5; Gelrite, 3; Noble agar, 6 and Bacto-agar, 8. Both the number and diameter of calluses were affected by type of solidifying agent and anther genotype

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Prem, Deepak, Subhadra Singh, Padam Prakash Gupta, Jaivir Singh, and Sish Pal Singh Kadyan. "Callus induction and de novo regeneration from callus in Guar (Cyamopsis tetragonoloba)." Plant Cell, Tissue and Organ Culture 80, no. 2 (2005): 209–14. http://dx.doi.org/10.1007/s11240-004-0738-9.

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Webb, D. T., F. Webster, B. S. Flinn, D. R. Roberts, and D. D. Ellis. "Factors influencing the induction of embryogenic and caulogenic callus from embryos of Piceaglauca and P. engelmanii." Canadian Journal of Forest Research 19, no. 10 (1989): 1303–8. http://dx.doi.org/10.1139/x89-200.

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Zygotic embryos of Piceaglauca (Moench) Voss from five half-sib seed families and P. engelmanii Parry from one half-sib family, collected on July 13 and 27 and August 24, were cultured in the presence of 2,4-dichlorophenoxyacetic acid, N6-benzyladenine, and sucrose ranging from 0.5 to 4% for the induction of embryogenic callus and the production of stable embryogenic callus lines. Embryogenic callus was induced from all three collections with all seedlots. The July 13 collection was two to four times more embryogenic than the later collections. Embryogenic callus was induced at all sucrose lev

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Pallavi G. Tandlepatil and Sangeeta R. Ahuja. "In vitro callus induction from a highly medicinal plant: Pterocarpus marsupium Roxb." World Journal of Advanced Research and Reviews 25, no. 1 (2025): 414–17. https://doi.org/10.30574/wjarr.2025.25.1.0007.

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The current study has focused on an efficient in vitro callus induction protocol for the Pterocarpus marsupium Roxb. plant. Explants from healthy plants, including leaves and stem nodes, were employed to induce callus formation. Murashige and Skoog medium supplemented with 2,4D resulted in the highest callus proliferation. Callus induction varies based on the type of explant and growth hormone concentration employed. Massive callus was achieved at 1.0 mg/L of 2, 4 D and BAP each utilizing a leaf explant.

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Kruglova, N. N. "CALLUS FORMATION AND CALLUSOGENESIS IN VITRO IN CEREALS: THE ROLE OF HORMONAL BALANCE (REVIEW)." Izvestia Ufimskogo Nauchnogo Tsentra RAN, no. 1 (March 28, 2022): 52–59. http://dx.doi.org/10.31040/2222-8349-2022-0-1-52-59.

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The most difficult fundamental problem of plant development biology remains morphogenesis as the totality of cell differentiation processes occurring in a developing organism with the formation of specialized tissues and organs. Prospective experimental systems in this field of research are callus cultures in vitro. The callus is an integrated system formed both exogenously (as a result of the proliferation of surface cells of various tissues of the plant organism) and endogenously (in the depth of these tissues); initially consists of homogeneous cells, gradually transformed into a system of

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Guo, Ge, and Byoung Ryong Jeong. "Explant, Medium, and Plant Growth Regulator (PGR) Affect Induction and Proliferation of Callus in Abies koreana." Forests 12, no. 10 (2021): 1388. http://dx.doi.org/10.3390/f12101388.

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Korean fir (Abies koreana E.H. Wilson) is a unique Pinaceae tree species endemic in Korea. In recent years, it is believed that climate change has caused many of them to die. Therefore, it has become extremely important to protect and preserve this tree species. In this study, the possibility of callus induction using different explants, media, and plant growth regulators (PGRs) was studied. After the dormancy period in May 2020, needles and stem segments that grew from the leaf buds as the explants were collected from one-year-old shoots. The explants were disinfected and subsequently transfe

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Kim, Myung-Uk, and Shin-Young Lee. "Induction and Cultivation of Callus from Aloe vera L." Food Engineering Progress 16, no. 3 (2012): 226–32. http://dx.doi.org/10.13050/foodengprog.2012.16.3.226.

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A callus formation from Aloe vera L. was inducted, and the tissue cultivation of induced callus was investigated. As a result of using a combined growth factors kinetin and NAA (or 2,4-D) varying from 0.5 to 30 μM and 3% sucrose in MS solid medium, the callus grew better than using either kinetin or auxins alone. Supplementation of 2,4-D in the combined growth factor formulation showed an enhanced callus growth. The maximum callus growth occurred when kinetin 5 μM and 2,4-D 30 μM were added in the MS medium. The optimal culture conditions for the callus growth were 25°C, pH 5.5 and 8-10 weeks

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Sivanesan, Iyyakkannu, Kim E. Kyoung, Kim M. Kyoung, Ko E. Young, and Se W. Park. "Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion." Horticultura Brasileira 33, no. 4 (2015): 441–47. http://dx.doi.org/10.1590/s0102-053620150000400006.

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ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoe

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