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Journal articles on the topic 'Callus'

Author: Grafiati

Published: 4 June 2021

Last updated: 25 July 2025

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1

Arif, Mahmoud B., and Houchang Khatamian. "IN VITRO EMBRYOGENESIS DERIVED FROM LEAF CALLUS OF `TIFFANY' ROSE." HortScience 25, no. 9 (1990): 1085G—1086. http://dx.doi.org/10.21273/hortsci.25.9.1085.

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Friable callus from leaf disks of Rosa hybrida `Tiffany' was initiated within two weeks under dark conditions and 25°C on Murashige and Skoog (MS) medium supplemented with 4 mg.liter-1 2,4-D. Callus was then transferred into MS medium containing 3 mg.liter-1 2,4-D. Within four weeks, rhizogenesis occurred on the callus surface. The rhizogenic calllus was subculture on MS medium plus 3 mg.liter-1 2,4-D every 4-6 weeks. Within six months from initial culture, somatic embryos were developed on the aging callus in darkness. Transfer of the aging callus with somatic embryos into 1/2 MS medium conta

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2

Arif, Mahmoud B., and Houchang Khatamian. "IN VITRO EMBRYOGENESIS DERIVED FROM LEAF CALLUS OF `TIFFANY' ROSE." HortScience 25, no. 9 (1990): 1085g—1086. http://dx.doi.org/10.21273/hortsci.25.9.1085g.

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Friable callus from leaf disks of Rosa hybrida `Tiffany' was initiated within two weeks under dark conditions and 25°C on Murashige and Skoog (MS) medium supplemented with 4 mg.liter-1 2,4-D. Callus was then transferred into MS medium containing 3 mg.liter-1 2,4-D. Within four weeks, rhizogenesis occurred on the callus surface.The rhizogenic calllus was subculture on MS medium plus 3 mg.liter-1 2,4-D every 4-6 weeks. Within six months from initial culture, somatic embryos were developed on the aging callus in darkness.Transfer of the aging callus with somatic embryos into 1/2 MS medium contain

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3

Dainty, A. L., K. H. Goulding, P. K. Robinson, I. Simpkins, and M. D. Trevan. "Callus conclusion." Trends in Biotechnology 3, no. 9 (1985): 219. http://dx.doi.org/10.1016/0167-7799(85)90010-1.

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4

Gannon, Francis H., and Lester D. R. Thompson. "Traumatic Fracture Callus." Ear, Nose & Throat Journal 86, no. 4 (2007): 200. http://dx.doi.org/10.1177/014556130708600407.

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5

Oni, O. A. "The bony callus." Injury 28, no. 9-10 (1997): 629–31. http://dx.doi.org/10.1016/s0020-1383(97)00126-5.

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6

Toker, G., M. Memişoğlu, M. C. Toker, and E. Yeşilada. "Callus formation and cucurbitacin B accumulation in Ecballium elaterium callus cultures." Fitoterapia 74, no. 7-8 (2003): 618–23. http://dx.doi.org/10.1016/s0367-326x(03)00165-5.

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7

Dovzhenko, Alexander, and Hans-Ulrich Koop. "Sugarbeet ( Beta vulgaris L.): shoot regeneration from callus and callus protoplasts." Planta 217, no. 3 (2003): 374–81. http://dx.doi.org/10.1007/s00425-003-1006-7.

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8

Famelaer, I., E. Ennik, W. Eikelboom, J. M. Van Tuyl, and J. Creemers-Molenaar. "The initiation of callus and regeneration from callus culture ofTulipa gesneriana." Plant Cell, Tissue and Organ Culture 47, no. 1 (1996): 51–58. http://dx.doi.org/10.1007/bf02318965.

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9

VALLES, M., and Ph BOXUS. "REGENERATION FROM ROSA CALLUS." Acta Horticulturae, no. 212 (September 1987): 691–96. http://dx.doi.org/10.17660/actahortic.1987.212.118.

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10

IKUTA, Akira. "Callus Constituents and Chemotaxonomy." Plant tissue culture letters 4, no. 1 (1987): 1–7. http://dx.doi.org/10.5511/plantbiotechnology1984.4.1.

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11

Benson, J. Christopher, and Alan S. Banks. "First Metatarsal Callus Distraction." Journal of the American Podiatric Medical Association 98, no. 1 (2008): 51–60. http://dx.doi.org/10.7547/0980051.

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We describe the results in seven patients who underwent surgery to lengthen a short first metatarsal via callus distraction. The increased length achieved ranged from 13 to 48 mm, with an average of 20.2 mm. The technique was successful in restoring length and improving symptoms, although several complications were encountered. (J Am Podiatr Med Assoc 98(1): 51–60, 2008)

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12

Gonzalez Benito, E., and P. G. Alderson. "REGENERATION FROM ALSTROEMERIA CALLUS." Acta Horticulturae, no. 280 (July 1990): 135–38. http://dx.doi.org/10.17660/actahortic.1990.280.21.

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13

Ellem, Bradon, and Russell Lansbury. "Tribute to Ron Callus." Journal of Industrial Relations 49, no. 2 (2007): 147–49. http://dx.doi.org/10.1177/0022185607074906.

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14

Palma, L. de, A. Tulli, G. Maccauro, S. P. Sabetta, and M. del Torto. "Fracture Callus in Osteopetrosis." Clinical Orthopaedics and Related Research &NA;, no. 308 (1994): 85???89. http://dx.doi.org/10.1097/00003086-199411000-00014.

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15

Jaramillo, J., and W. L. Summers. "Tomato Anther Callus Production: Solidifing Agent and Concentration Influence Induction of Callus." Journal of the American Society for Horticultural Science 115, no. 6 (1990): 1047–50. http://dx.doi.org/10.21273/jashs.115.6.1047.

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Anthers from. three tomato cultivars, `L-680A', `Ailsa Craig', and `Licato', were plated on DBM1 medium solidified with one of four solidifying agents, Bacto-agar, Gelrite, Noble agar, or Phytagar, to evaluate their ability to promote initiation and growth of tomato anther callus. The optimum concentration of each solidifying agent was compared with a liquid control. Optimum levels of the various solidifying agents were (in g·liter-1) Phytagar, 5; Gelrite, 3; Noble agar, 6 and Bacto-agar, 8. Both the number and diameter of calluses were affected by type of solidifying agent and anther genotype

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16

ABE, Toshinori, and Yuzo FUTSUHARA. "Diallel analysis of callus growth and plant regeneration in rice seed-callus." Japanese Journal of Genetics 66, no. 2 (1991): 129–40. http://dx.doi.org/10.1266/jjg.66.129.

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17

Prem, Deepak, Subhadra Singh, Padam Prakash Gupta, Jaivir Singh, and Sish Pal Singh Kadyan. "Callus induction and de novo regeneration from callus in Guar (Cyamopsis tetragonoloba)." Plant Cell, Tissue and Organ Culture 80, no. 2 (2005): 209–14. http://dx.doi.org/10.1007/s11240-004-0738-9.

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18

Haris, Muhammad, Sumbal Rasheed, Shahroz Rahman, Ishmal Fatima, Ayesha Mazhar, and Farrukh Azeem. "Tissue Culture Optimization: Maize Callus Induction and Factors Affecting the Callus Efficiency." Integrative Plant Biotechnology 3, no. 2 (2025): 73–99. https://doi.org/10.55627/pbiotech.003.02.1288.

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Maize (Zea mays L.), also known as the queen of cereals, is one of the most important food crops in terms of food security, the world economy, and global agriculture. The high yield, diverse climate adaptability, and extensive applications of maize in food, feed, and industrial products make it a highly demanding crop. The traditional methods of maize propagation are slow and often result in less yield with undesired traits due to large-scale variations. However, the advancements in agricultural biotechnology, particularly in plant tissue culture, have made it possible to propagate maize cultu

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19

Qin, Yan Jun, Wu Que Gong, Ting Liu, Jing Hui Yang, Yan Jun Liu, and Wei Zi Huang. "Difference of Morphological Structure and Characteristics of Physiology and Biochemistry between Two Types of Alfalfa Callus." Applied Mechanics and Materials 700 (December 2014): 306–9. http://dx.doi.org/10.4028/www.scientific.net/amm.700.306.

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In order to know the key factors of callus development on single cell clone, characteristics of physiology and biochemistry and morphological structure on two types of Alfalfa callus was studied. The results showed that the cell membrane permeability and malondialdehyde content was same on two types of callus; but, their soluble sugar content and peroxidase activity in cells was larger different, the soluble sugar content: soft callus > huge callus, and soft callus was 2.09 times of huge callus; peroxidase activity: huge callus > soft callus, and huge callus was 1.35 times of soft callus

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20

Hu, Yan Yan, Jing Hui Yang, Chao Zhang, Ting Liu, Bin Li, and Ying Wang. "Difference of Cell Morphology on Different Callus Types of Alfalfa." Applied Mechanics and Materials 707 (December 2014): 137–43. http://dx.doi.org/10.4028/www.scientific.net/amm.707.137.

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In order to induce embryo from callus and set up the integral somatic embryo induction system from monoclonal cell, the differences of cell morphology and structure from different types of alfalfa callus were observed and compared by quick section and microscopic examination. The results show that giant callus cells were larger, elongated and yellow-white; loose callus cells were smaller, spherical, soft and yellow-green; hard-type callus cells were round, hard and dark green. The cell volume of giant callus was 4.5 times more than loose callus cells and 9 times more than hard-type callus cell

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21

Yang, Yali, Peiting He, Jiayi Zhang, et al. "Induction of callus in Cassia mimosoides." E3S Web of Conferences 189 (2020): 02017. http://dx.doi.org/10.1051/e3sconf/202018902017.

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In this study, the young leaves of Cassia mimosoides were used as explants. On the basis of 1/2 MS +2.0 mg/L 6-BA +0.5 mg/L NAA medium, we observed the effect of adding different factors on the callus induction of C. mimosoides. The results shown that after 20 days of callus induction, it was found that the addition of 150 mg/L vitamin C (VC) did not affect the induction efficiency, but the growth of callus became slower; and when the concentration was higher than 150 mg/L, the formation of callus would be inhibited. The induction of callus would be restrained while adding different concentrat

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22

Adkins, SW. "Cereal Callus Cultures: Control of Headspace Gases Can Optimise the Conditions for Callus Proliferation." Australian Journal of Botany 40, no. 6 (1992): 737. http://dx.doi.org/10.1071/bt9920737.

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The protective conditions under which callus cultures are grown to prevent microbial contamination and to reduce tissue desiccation cause the accumulation of volatiles in the vessel headspace and reduce the availability of oxygen for respiration. To demonstrate the importance of the gaseous atmosphere to culture growth a study was undertaken on non-morphogenic rice and wheat callus incubated under a number of environmental conditions. Changes in the gaseous atmosphere above rice (Oryza sativa L.) callus during routine culture in a petri dish suppressed growth and promoted necrosis. Incubating

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23

Khairan, Khairan, Betty Mauliya Bustam, Yunita, Riska Meilinda, and Raudhatul Muna. "Pengaruh Komposisi 2,4-D dan BAP Terhadap Pembentukan Kalus Eksplan Pucuk Nilam (Pogostemon Cablin Benth.) secara In Vitro dengan Pemotongan Horizontal dan Vertikal." Biotropic : The Journal of Tropical Biology 5, no. 1 (2021): 9–20. http://dx.doi.org/10.29080/biotropic.2021.5.1.9-20.

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This study aims to determine the effect of 2,4-Diclorophenoxy Acetic Acid (2,4-D) and Benzyl Amino Purin (BAP) on the formation of callus of patchouli (Pogostemon cablin Benth.) shoot explants by horizontal and vertical cutting methods. The parameters that observed in this study were the percentage growth of callus, time appearance of callus, weight of callus and the morphology of callus. The results showed that horizontal cutting method was able to induce callus growth with the percentages growth of callus were 18,75%, with the time appearance of callus was at 16 days at P1; P10; P12; P13 dan

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24

Chée, Raymond P., Daniel I. Leskovar, and Daniel J. Cantliffe. "Optimizing Embryogenic Callus and Embryo Growth of a Synthetic Seed System for Sweetpotato by Varying Media Nutrient Concentrations." Journal of the American Society for Horticultural Science 117, no. 4 (1992): 663–67. http://dx.doi.org/10.21273/jashs.117.4.663.

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Embryogenic callus growth of sweetpotato [Ipomoea batatas (L.) Lam.] was selectively enhanced by subculture on basal callus proliferation medium modified to contain 15 mm NH4NO3. Embryogenic callus production was doubled on basal callus proliferation medium modified to contain 60 mm K+, while nonembryogenic callus production was reduced 40%. Additions of up to 40 mm NaCl to basal callus proliferation medium did not affect callus proliferation. The development of embryos from calli subculture to embryo production basal medium was unaffected by the KCl or NaCl treatments of the callus proliferat

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25

Fransz, P. F., and J. H. N. Schel. "Cytodifferentiation during the development of friable embryogenic callus of maize (Zea mays)." Canadian Journal of Botany 69, no. 1 (1991): 26–33. http://dx.doi.org/10.1139/b91-005.

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Immature embryos of Zea mays L. were cultured on N6 medium to obtain embryogenie callus. Cultured tissue fragments with various developmental stages of friable callus were sampled and prepared for light microscopy and transmission electron microscopy. Pieces of compact callus were also prepared for light microscopy to compare the structural organization of both callus types. Friable callus develops from a thin layer of abaxial scutellum cells, including the epidermis. During further development the callus cells dissociate, owing to the breakdown of the middle lamellae, while older vacuolated c

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26

MATSUO, Masaki, Toshitaka UCHINO, and Hidemitsu MASUDA. "Effect of Electric Current Flow to Callus on the Multiplication of Asparagus Callus." Shokubutsu Kojo Gakkaishi 4, no. 1 (1992): 10–14. http://dx.doi.org/10.2525/jshita.4.10.

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27

WIJAYA, BARAELA EZRA, RINDANG DWIYANI, and NI NYOMAN ARI MAYADEWI. "Induksi dan Multiplikasi Kalus Eucalyptus sp. pada Berbagai Media Callus Induction Medium (CIM) Secara In Vitro." Agrotrop : Journal on Agriculture Science 13, no. 1 (2023): 76. http://dx.doi.org/10.24843/ajoas.2023.v13.i01.p07.

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Callus Induction and Multiplication of Eucalyptus sp. on Various Media Callus Induction Medium (CIM) In Vitro. Callus induction in vitro can be done by planting specific part of plant such as leaf, root, stem, etc in aseptic condition by using medium that contains 2,4-D, a plat regulator for promoting callus formation. One of the objectives of callus production in vitro is to form secondary metabolites, because eucalyptus is known as a producer of essential oils. This research is an initial study for in vitro production of secondary metabolites for further research. The aim of this study was t

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28

Hidayat, Rahmad, Abdullah Abdullah, and Aminah Aminah. "PEMANTAPAN MASA INKUBASI KALUS KEDELAI SECARA IN VITRO DALAM MEDIA MS (Murashige dan Skoog) B5 (Gamborg)." AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Peranian 2, no. 2 (2022): 60–70. http://dx.doi.org/10.33096/agrotekmas.v2i2.192.

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Combination of Soybean Callus Incubation Period in vitro in MS (Murashige and Skoog) and B5 (Gamborg) Media (Supervised by Abdullah and Aminah). This study aimed to find a suitable medium for stabilizing the incubation period of soybean callus in vitro for the purposes of further selection. This research was conducted at the Laboratory of Bio-Science and Plant Reproduction Biotechnology, Agronomy Department, Agriculture Faculty, Hasanuddin University, Makassar, South Sulawesi. This research was done from June 2020 to October 2020. This research was conducted using a Completely Randomized Desig

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29

Tan, Yanping, Wen Hu, Xin Xu, et al. "Polyamine Plays a Role in Subculture Growth of in Vitro Callus of Indica Rice." Acta Biologica Cracoviensia s. Botanica 59, no. 1 (2017): 105–12. http://dx.doi.org/10.1515/abcsb-2017-0001.

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AbstractIn vitroembryogenic callus is a critical factor for genetic transformation of rice, especially for indica varieties. In this study, we investigated the relationship between polyamines, including putrescine (Put), spermidine (Spd) and spermine (Spm), and callus browning, and we studied the effect of exogenous Put on callus regeneration and on the content of endogenous polyamines. In addition, the expression levels of arginine decarboxylase gene (Adc1) and S-adenosylmethionine decarboxylase gene (Samdc) in embryogenic callus were studied by quantitative Real-time PCR analysis. The result

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30

Agha, Hasan M., Khairul Adzfa Radzun, Norrizah Jafaar Sidik, and Ali H. Jawad. "Callus Induction of Fenugreek Trigonella Foenum-Graecum via Auxin Combined with Cytokinins Hormones, and Assessment of Toxicity via Brine Shrimp Assay." Journal of Asian Scientific Research 12, no. 1 (2022): 12–27. http://dx.doi.org/10.55493/5003.v12i1.4449.

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Trigonella foenum-graecum (TFG) is a significant leguminous plant with diverse pharmacological effects. However, the resistant character of this plant accounts for significant difficulties in vitro multiplication, justifying the necessity to try new techniques for in vitro propagation of this plant. Hence, this study reports the effects of BAP, NAA, and 2,4-D on in vitro callus formation of seeds. The toxicity properties of seeds, plant, and callus aqueous extracts of TFG were measured by brine shrimp assay (BSA). Callus index, frequency of callus, callus weight, and morphology of callus were

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31

Habibah, Noor Aini, Ananda Lutfiah, Alin Liana, Woro Anindito Sri Tunjung, Meti Indrowati, and Furzani Pa’ee. "Callogenesis of Dayak Onion (Eleutherine palmifolia) Bulb in response of Picloram, 2,4-D, and Kinetin." Biosaintifika: Journal of Biology & Biology Education 15, no. 2 (2023): 270–80. http://dx.doi.org/10.15294/biosaintifika.v15i2.46501.

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Dayak onion contains bioactive compounds that are traditionally used for medicine. The production of bioactive compounds can be increased through callus culture. This study aims to analyze the effect of the combination of plant growth regulator on the growth of Dayak bulb callus. The design of this study used a completely randomized factorial design with 2 factors. The first factor is the concentration of auxin. While the second factor is the concentration of kinetin. The parameters observed include the percentage of callus, the time of callus formation, fresh weight, dry weight, and callus mo

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32

Mackay, Wayne A., Timothy J. Ng, and Freddi A. Hammerschlag. "Cucumis melo L. Callus Response to Toxins Produced by Myrothecium roridum Tode ex. Fries." Journal of the American Society for Horticultural Science 119, no. 2 (1994): 356–60. http://dx.doi.org/10.21273/jashs.119.2.356.

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Studies examining exposure methods and callus type were conducted to develop an in vitro selection system using roridin E as a selection agent. Vacuum infiltration of callus with the toxin solution was the only successful selection method at the concentrations tested. Primary callus (callus originating directly from the explant) was not sensitive to roridin A or E at the concentrations used. Secondary callus (callus produced from primary callus) exhibited a differential response to roridins A and E similar to that of detached-leaf assays. Electrolyte leakage studies of callus were not conclusi

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33

Muralitharan, MS, SF Chandler, and RFMV Steveninck. "Physiological Adaptation to High Ion Concentrations or Water Deficit by Callus Cultures of Highbush Blueberry, Vaccinium corymbosum." Functional Plant Biology 20, no. 2 (1993): 159. http://dx.doi.org/10.1071/pp9930159.

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Callus cultures of highbush blueberry were selected for 10 passages on medium supplemented with 50 rnol m-3 NaCl, 50 rnol m-3 KCl, 25 mol m-3 Na2SO4, 25 mol m-3 K2SO4 or 100 mol m-3 mannitol. On all salts, growth of selected callus was greater (200-250%) than that of non-selected callus, and selected callus grew optimally on the type of salt on which it was selected. Conventional (whole plant analysis) and electron probe X-ray microanalysis showed that selected callus accumulated more ions (approximately 1.5-3.0-fold) than non-selected callus on all salts, and there was a positive correlation

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34

Kim, Jaewook, Jung-Woo Lee, and Ick-Hyun Jo. "Multiple Transcriptomic Networks Regulate the Callus Development Process in Panax ginseng." Agronomy 15, no. 5 (2025): 1244. https://doi.org/10.3390/agronomy15051244.

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Callus induction is one of the most important techniques in plant-based industries. Important features in the use of callus induction are the maintenance of pluripotency and the proliferation of cells. Although the importance of callus induction is also understood in ginseng, there are no studies on the genetic modules associated with callus induction and growth regulation. Panax ginseng embryo tissue was wounded and cultured in callus-inducing media, and its time-course physiology was observed. Time-course callus samples were collected for total RNA extraction and RNA-Seq analysis using the I

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35

Schneider, K. E., D. Speranzini, and A. R. Biggs. "Ontogeny of shoot regenerants on excised immature peach embryos." Canadian Journal of Plant Science 72, no. 2 (1992): 497–506. http://dx.doi.org/10.4141/cjps92-063.

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Excised immature peach embryos when cultured on an auxin-containing medium produced both a friable callus and a nodular callus. Upon further subculturing on an auxin-reduced medium only the nodular callus differentiated into highly regenerative nodular callus. This callus comprised dense meristematic cells which arose near or at the surface of the nodular tissue. Cells containing starch granules often surrounded these meristemoid areas. Shoots could be induced to form on shoot-inducing medium from the meristemoid areas. Vascular connections developed between the parental material and the shoot

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36

Mureșan, Liliana, Doina Clapa, Teodor Rusu, Thomas T. Y. Wang, and Jae B. Park. "Soybean Callus—A Potential Source of Tocopherols." Plants 10, no. 12 (2021): 2571. http://dx.doi.org/10.3390/plants10122571.

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In vitro cultures have been used as an effective means to achieve a high level of secondary metabolites in various plants, including soy. In this study, the contents of α-, γ-, and δ- tocopherol were quantified in soybean callus, and their amounts were compared to those of soybeans cultivated using the conventional tillage system with three weed controls (respectively without herbicide and with two variants of herbicide). Soybean callus was produced using Murashige and Skoog 1962 (MS) medium supplemented with 0.1 mg/L 6-Benzylaminopurine (BAP) and 0. 1 mg/L Thidiazuron (TDZ). The highest amoun

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37

Adju, Fitria Y. Hs, Jusna Ahmad, Devi Bunga Pagalla, Novri Youla Kandowangko, Indriati Husain, and Lindawaty Isima. "In Vitro Callus Induction from (Antidesma bunius L.) Leaves." BIOVALENTIA: Biological Research Journal 11, no. 1 (2025): 40–46. https://doi.org/10.24233/biov.11.1.2025.451.

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Antidesma bunius L. is a tree species that has ecological and economic benefits, and has many bioactivities. Propagation of this tree can be done in several ways, one of which is callus induction. Callus is a collection of undifferentiated cells that can develop into new plants with the help of ZPT. The purpose of this study was to determine the effect and optimum combination of ZPT 2,4-D and BAP on the induction of leaf callus of A. bunius L. The research design was a non-factorial Completely Randomised Design (CRD) with 4 treatments namely P0: MS without the addition of zpt; P1: MS + 1 ppm B

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38

Xiang, Bei Bei, Ye Rong Zhu, Wen Juan Wang, Yan Ling Bai, and Yong Wang. "Improvement of Vindoline Production in a Catharanthus roseus callus Line." Advanced Materials Research 345 (September 2011): 375–80. http://dx.doi.org/10.4028/www.scientific.net/amr.345.375.

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The influence of polyploidity on the accumulation of vindoline in Catharanthus roseus callus cultures was investigated. The callus line (T1) was induced from a tetraploid leaf. Total alkaloids in the callus were extracted and analyzed by LC-MS for qualitification and HPLC for quantification. Results showed that T1 callus cultures could accumulate vindoline to a high level. The highest accumulation of vindoline found in the callus was 0.11 mg g−1 DW. Our results demonstrate that polyploidity could influence the chlorophyll content or chloroplast development and improve vindoline biosynthesis in

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39

Royani, J. I., H. Khairiyah, F. R. Mira, Karyanti, and N. Watanabe. "The effect of callus induction media for somatic embryo formation in Hevea brasiliensis Muell. Arg." IOP Conference Series: Earth and Environmental Science 1160, no. 1 (2023): 012007. http://dx.doi.org/10.1088/1755-1315/1160/1/012007.

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Abstract The method for micro-propagation of rubber (Hevea brasiliensis Muell. Arg) by somatic embryo has been conducted. The aim of the research will describe the effect of media used for callus induction on the somatic embryo formation of rubber plants. Embryonic callus had been induction in 3 media, i.e.; MH1, MH2, and MH3. After callus induction, all callus were induced in media for embryo somatic expression. Maturation of cotyledons was performed using liquid media with filter paper as a bridge to absorb the media. The results showed that the percentage of callus was induced differently f

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40

Anjani, Dianita Dewi, and Ratnawati. "The Effect of BAP and NAA Combination on Callus Induction of Aglaonema Siam Aurora Leaf Explants in Vitro." Indonesian Journal of Bioscience (IJOBI) 1, no. 2 (2023): 85–92. http://dx.doi.org/10.21831/ijobi.v1i2.213.

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This research aims to determine the effect of the Naphthalene Acetic Acid and Benzyl Amino Purine combination on callus growth of Aglaonema Siam Aurora leaf explants and to determine the combination level at the most optimal concentration on the callus growth of Aglaonema Siam Aurora leaf explants. Observation variables included callus emergence time, explants percentage of formed callus, callus length, and explant life percentage. The explants that where used were the first young leaves explants from shoots that were opened, with 2x2 cut size. Results show that the addition of a NAA and BAP c

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Yang, Lin, Jinge Sun, Congyu Yan, et al. "Regeneration of duckweed (Lemna turonifera) involves genetic molecular regulation and cyclohexane release." PLOS ONE 17, no. 1 (2022): e0254265. http://dx.doi.org/10.1371/journal.pone.0254265.

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Plant regeneration is important for vegetative propagation, detoxification and the obtain of transgenic plant. We found that duckweed regeneration could be enhanced by regenerating callus. However, very little is known about the molecular mechanism and the release of volatile organic compounds (VOCs). To gain a global view of genes differently expression profiles in callus and regenerating callus, genetic transcript regulation has been studied. Auxin related genes have been significantly down-regulated in regenerating callus. Cytokinin signal pathway genes have been up-regulated in regeneratin

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Huda, Moch Faizul, and Serafinah Indriyani. "Effect of Benzyl Adenine Concentration on Callus Induction of Geranium Plants (Pelargonium graveolens L'Her) from Petiole and Leaf Explants." Journal of Experimental Life Sciences 10, no. 1 (2020): 20–23. http://dx.doi.org/10.21776/ub.jels.2019.010.01.04.

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Geranium plant (Pelargonium graveolens L'Her) is one of the geranium oil producing plants that has many benefits. Callus culture is a technique that can be used to plant multiplication and increase production of secondary metabolites. This study aims to determine the effect of the concentration of Benzyl Adenine on the formation of geranium callus from petiole and leaf explants. Callus induction was carried out by culturing petiole and leaf explants on MS medium + 0.1 mg.L-1 NAA + Benzyl Adenine (0; 0.5; 1; 1.5 and 2 mg.L-1). Callus morphological parameters, percentage of callus formation, and

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Rajbhandari, Nirmala, and Anne-Marie Stomp. "Embryogenic Callus Induction in Fraser Fir." HortScience 32, no. 4 (1997): 737–38. http://dx.doi.org/10.21273/hortsci.32.4.737.

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Mass propagation of Fraser fir [Abies fraseri (Pursh) Poir], a valuable Christmas tree species in the United States, is problematic because methods currently used are inadequate. According to our results with somatic embryogenesis, the culturing methods used with other Abies species are applicable to Fraser fir. Stage 1 somatic embryogenic callus, characterized by suspensor cells and embryo heads, was obtained at low frequency using Schenk and Hildebrandt medium supplemented with 5 mm glutamine, 0.05% casein hydrolysate, 0.01% myoinositol, 2% sucrose, 5 μM benzyladenine, and 0.6% agar. The dev

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Revutskaya, A. Z., A. V. Holubenko, N. V. Nuzhyna, H. O. Rudik, and N. Yu Taran. "Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)." Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv 17, no. 1 (2019): 33–37. http://dx.doi.org/10.7124/visnyk.utgis.17.1.1198.

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Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical proce

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Galibina, N. A., M. A. Ershova, R. V. Ignatenko, K. M. Nikerova, I. N. Sofronova, and M. N. Borodina. "Cytogenetic and Biochemical Characteristics of Callus <i>Pinus sylvestris</i> L." Физиология растений 70, no. 1 (2023): 100–112. http://dx.doi.org/10.31857/s0015330322100244.

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A comprehensive assessment was carried out of the changes occurring during the darkening of the callus obtained from vegetative buds of 40-year-old Scots pine treesPinus sylvestrisL. Based on biometric assessment of callus (intensity of callus formation, proportion of light callus, callus mass) from 32 analyzed trees (16 genotypes represented by two clones), two genotypes with high callus-forming ability were singled out. Analysis of mitosis showed that, although the proportion of aberrant cells in the callus does not exceed the rate of spontaneous mutation forP. sylvestris, the range of viola

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Setiaji, Arkan, RR Rifka Annisa, Rumiyati Rumiyati, and Endang Semiarti. "Induction and Growth Kinetics Callus of Tomato (Solanum lycopersicum)." Biosaintifika: Journal of Biology & Biology Education 12, no. 1 (2020): 35–41. http://dx.doi.org/10.15294/biosaintifika.v12i1.21704.

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Plant callus extracts are potential to be developed as ingredient in skincare products. Tomato callus is supposed to contain protein-derivatives and or other components such as secondary metabolites that play a role in skin regeneration. Therefore the production of calli is important to be studied for callus sustainable supply. This research aims to obtain optimum medium for callus induction and to analyze tomato callus development anatomically. In vitro culture response was assessed in tomato plant (Solanum lycopersicum L. ‘Permata’) for optimum callus induction. Seeds were grown on ¼ MS medi

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Özgen, Murat, Melahat A. Birsin, and Berk Benlioglu. "Biotechnological characterization of a diverse set of wheat progenitors (Aegilops sp. and Triticum sp.) using callus culture parameters." Plant Genetic Resources 15, no. 1 (2015): 45–50. http://dx.doi.org/10.1017/s1479262115000350.

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It is known that genetic diversity is the most important factor in classical and modern plant breeding. The considerable increase in the number of transgenic crops reveals the value of new plant genetic resources. In this study, a set of 12 wheat progenitors were screened for tissue culture parameters such as callus induction, callus weight, regeneration capacity of callus and callus efficiency using mature embryos. Embryos were excised from imbibed seeds of the progenitors. The excised embryos were placed scutellum upwards in dishes containing 2 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D) for

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HARDIYANTO, ARIF, SOLICHATUN SOLICHATUN, and WIDYA MUDYANTINI. "The effect of varied NAA concentration agains growth and flavonoid content of Gynura procumbens (Lour) Merr. callus." Biofarmasi Journal of Natural Product Biochemistry 2, no. 2 (2004): 69–74. http://dx.doi.org/10.13057/biofar/f020205.

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The aim of this research is to study the influence of naphthalene acetic acid (NAA) concentration variation in the callus growth and flavonoid content of daun dewa [Gynura procumbens (Lour) Merr.]. The research framework relied on the benefit of daun dewa as anti cancer drug. This matter enabled by its compound cytotoxic from compound flavonoid contained. Culture in vitro technique serves the purpose of medium to produce the secondary metabolism. Addition of NAA as treatment for the induction of callus will promote cell enlargement and the growth of callus so that callus which later will be an

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Economou, A. S., Paul E. Read, and Maria J. Spanoudaki. "AZALEA REGENERATION FROM CALLUS CULTURE." Acta Horticulturae, no. 226 (June 1988): 209–16. http://dx.doi.org/10.17660/actahortic.1988.226.24.

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NAKAMURA, Tiemi, Nobuhiro HIGUCHI, and Eizo MAEDA. "STRUCTURE OF RICE CALLUS TISSUES." Plant tissue culture letters 2, Supplement (1985): 100. http://dx.doi.org/10.5511/plantbiotechnology1984.2.supplement_100.

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